Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059926.t1 | XP_013457865.1 | 96.1 | 233 | 9 | 0 | 1 | 233 | 67 | 299 | 1.30E-103 | 386 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059926.t1 | Q9STS6 | 26.8 | 239 | 129 | 5 | 1 | 196 | 93 | 328 | 7.8e-09 | 62.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059926.t1 | A0A072URD2 | 96.1 | 233 | 9 | 0 | 1 | 233 | 67 | 299 | 9.2e-104 | 386.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene059926.t1 | TF | LOB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059926.t1 | MTR_4g107450 | 98.283 | 233 | 4 | 0 | 1 | 233 | 67 | 299 | 1.36e-172 | 476 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059926.t1 | AT3G47870 | 30.417 | 240 | 119 | 8 | 1 | 196 | 93 | 328 | 1.10e-16 | 78.2 |
Find 42 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATCATTCAAGCTAATTAT+AGG | 0.238366 | 8.1:-19551055 | MS.gene059926:CDS |
| GTGACTTCACGGTTAGAATT+AGG | 0.257746 | 8.1:-19550885 | MS.gene059926:CDS |
| ATCAACAACTTGAAATTTGC+AGG | 0.281961 | 8.1:-19550944 | MS.gene059926:CDS |
| GCAGGCTACAATATCAAATA+TGG | 0.297786 | 8.1:-19550995 | MS.gene059926:CDS |
| GCAATCATACATTTATTCTA+AGG | 0.300515 | 8.1:-19550505 | MS.gene059926:CDS |
| CTGCTATTATTATTTGTGTA+AGG | 0.327541 | 8.1:+19550654 | None:intergenic |
| TATCATTCAAGCTAATTATA+GGG | 0.334002 | 8.1:-19551054 | MS.gene059926:CDS |
| GGGCACTGAAGCCACAAATT+AGG | 0.358880 | 8.1:+19550675 | None:intergenic |
| TTGTTATAAGTCTGTGGTTG+TGG | 0.395121 | 8.1:+19550816 | None:intergenic |
| TATCTTCAACAACTCCTTGT+TGG | 0.395214 | 8.1:+19550571 | None:intergenic |
| AAAGAGCGCGAGCGCATTGA+AGG | 0.402476 | 8.1:+19550848 | None:intergenic |
| CATCATGAGGTAACTGTTGT+TGG | 0.408383 | 8.1:+19550910 | None:intergenic |
| ATGCTACAAATCCTAATTTG+TGG | 0.446980 | 8.1:-19550686 | MS.gene059926:CDS |
| CTCATGATGATGTGACTTCA+CGG | 0.450966 | 8.1:-19550896 | MS.gene059926:CDS |
| GATGTTGTGAGGAAATTTGC+AGG | 0.467829 | 8.1:-19551013 | MS.gene059926:CDS |
| TCGGAAGTAACCAACTTATG+AGG | 0.481637 | 8.1:+19550615 | None:intergenic |
| AGATGATAGTGGCTGTGATT+CGG | 0.490324 | 8.1:+19550596 | None:intergenic |
| ACAATATCAAATATGGATGA+TGG | 0.492612 | 8.1:-19550988 | MS.gene059926:CDS |
| TGCTATTATTATTTGTGTAA+GGG | 0.504837 | 8.1:+19550655 | None:intergenic |
| ATAATCCACACTATTGCTGT+TGG | 0.518450 | 8.1:+19550743 | None:intergenic |
| AGCAATGCAACCTCATAAGT+TGG | 0.519249 | 8.1:-19550625 | MS.gene059926:CDS |
| TAATAACTCCTCCCTCTACA+TGG | 0.525658 | 8.1:-19550721 | MS.gene059926:CDS |
| TTGAATGATAATAGAATCCA+TGG | 0.528060 | 8.1:+19551067 | None:intergenic |
| CTCCCTCTACATGGATTCAA+AGG | 0.529912 | 8.1:-19550712 | MS.gene059926:CDS |
| ATCATACATTTATTCTAAGG+AGG | 0.554320 | 8.1:-19550502 | MS.gene059926:CDS |
| CTGATCAGTTGACACTGGTA+AGG | 0.557706 | 8.1:+19550093 | None:intergenic |
| TTATTGTTGTTATAAGTCTG+TGG | 0.569885 | 8.1:+19550810 | None:intergenic |
| TCCTCACAACATCCATGCAC+AGG | 0.573785 | 8.1:+19551023 | None:intergenic |
| CACACTATTGCTGTTGGAGT+AGG | 0.575688 | 8.1:+19550749 | None:intergenic |
| CCTTGTTGGACAGATGATAG+TGG | 0.578651 | 8.1:+19550585 | None:intergenic |
| AGGGATAAGTATCCTGTGCA+TGG | 0.583412 | 8.1:-19551035 | MS.gene059926:CDS |
| ATCCTTTGAATCCATGTAGA+GGG | 0.590065 | 8.1:+19550710 | None:intergenic |
| TTCACGGTTAGAATTAGGAA+TGG | 0.610609 | 8.1:-19550880 | MS.gene059926:CDS |
| CTACTCCAACAGCAATAGTG+TGG | 0.620940 | 8.1:-19550748 | MS.gene059926:CDS |
| CATCCTTTGAATCCATGTAG+AGG | 0.632560 | 8.1:+19550709 | None:intergenic |
| CCACTATCATCTGTCCAACA+AGG | 0.649871 | 8.1:-19550585 | MS.gene059926:CDS |
| CTAAGGAGGCTTATGAATCG+AGG | 0.657710 | 8.1:-19550488 | MS.gene059926:intron |
| CGTGAAGTCACATCATCATG+AGG | 0.660061 | 8.1:+19550897 | None:intergenic |
| TAGTACTGATCAGTTGACAC+TGG | 0.662425 | 8.1:+19550088 | None:intergenic |
| CTTTGAATCCATGTAGAGGG+AGG | 0.666923 | 8.1:+19550713 | None:intergenic |
| TCCTGTGCATGGATGTTGTG+AGG | 0.692042 | 8.1:-19551024 | MS.gene059926:CDS |
| TGTTGCTGAGATACAGACAA+AGG | 0.703585 | 8.1:+19550774 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTGAAATTAGATATAG+TGG | - | chr8.1:19550875-19550894 | MS.gene059926:CDS | 20.0% |
| !! | ATGTTGAAATTAGATATAGT+GGG | - | chr8.1:19550876-19550895 | MS.gene059926:CDS | 20.0% |
| !! | TATCATTCAAGCTAATTATA+GGG | - | chr8.1:19550108-19550127 | MS.gene059926:CDS | 20.0% |
| !! | TATTGTTTGATCTACTTTAA+CGG | + | chr8.1:19550738-19550757 | None:intergenic | 20.0% |
| !! | TGCTATTATTATTTGTGTAA+GGG | + | chr8.1:19550510-19550529 | None:intergenic | 20.0% |
| !! | TTATCATTCAAGCTAATTAT+AGG | - | chr8.1:19550107-19550126 | MS.gene059926:CDS | 20.0% |
| ! | ACAATATCAAATATGGATGA+TGG | - | chr8.1:19550174-19550193 | MS.gene059926:CDS | 25.0% |
| ! | CTGCTATTATTATTTGTGTA+AGG | + | chr8.1:19550511-19550530 | None:intergenic | 25.0% |
| ! | GCAATCATACATTTATTCTA+AGG | - | chr8.1:19550657-19550676 | MS.gene059926:CDS | 25.0% |
| !! | ATCATACATTTATTCTAAGG+AGG | - | chr8.1:19550660-19550679 | MS.gene059926:CDS | 25.0% |
| !! | TTATTGTTGTTATAAGTCTG+TGG | + | chr8.1:19550355-19550374 | None:intergenic | 25.0% |
| !!! | TTTTTGATACAGTTGATGAT+AGG | - | chr8.1:19550635-19550654 | MS.gene059926:CDS | 25.0% |
| ATCAACAACTTGAAATTTGC+AGG | - | chr8.1:19550218-19550237 | MS.gene059926:intron | 30.0% | |
| ATGCTACAAATCCTAATTTG+TGG | - | chr8.1:19550476-19550495 | MS.gene059926:intron | 30.0% | |
| ATAATCCACACTATTGCTGT+TGG | + | chr8.1:19550422-19550441 | None:intergenic | 35.0% | |
| ATACATTTCTGTGTGCAGTT+CGG | - | chr8.1:19550967-19550986 | MS.gene059926:CDS | 35.0% | |
| ATCCTTTGAATCCATGTAGA+GGG | + | chr8.1:19550455-19550474 | None:intergenic | 35.0% | |
| CTATATGATCATTGATCCAC+AGG | - | chr8.1:19550768-19550787 | MS.gene059926:CDS | 35.0% | |
| GCAGGCTACAATATCAAATA+TGG | - | chr8.1:19550167-19550186 | MS.gene059926:CDS | 35.0% | |
| TATCTTCAACAACTCCTTGT+TGG | + | chr8.1:19550594-19550613 | None:intergenic | 35.0% | |
| TTCACGGTTAGAATTAGGAA+TGG | - | chr8.1:19550282-19550301 | MS.gene059926:intron | 35.0% | |
| TTGTTATAAGTCTGTGGTTG+TGG | + | chr8.1:19550349-19550368 | None:intergenic | 35.0% | |
| AGCAATGCAACCTCATAAGT+TGG | - | chr8.1:19550537-19550556 | MS.gene059926:CDS | 40.0% | |
| ATCCCATTAGTACGATCACT+CGG | + | chr8.1:19550846-19550865 | None:intergenic | 40.0% | |
| CATCATGAGGTAACTGTTGT+TGG | + | chr8.1:19550255-19550274 | None:intergenic | 40.0% | |
| CATCCTTTGAATCCATGTAG+AGG | + | chr8.1:19550456-19550475 | None:intergenic | 40.0% | |
| GAACTGCACACAGAAATGTA+TGG | + | chr8.1:19550968-19550987 | None:intergenic | 40.0% | |
| GATGTTGTGAGGAAATTTGC+AGG | - | chr8.1:19550149-19550168 | MS.gene059926:CDS | 40.0% | |
| GTGACTTCACGGTTAGAATT+AGG | - | chr8.1:19550277-19550296 | MS.gene059926:intron | 40.0% | |
| TAATAACTCCTCCCTCTACA+TGG | - | chr8.1:19550441-19550460 | MS.gene059926:intron | 40.0% | |
| TCGGAAGTAACCAACTTATG+AGG | + | chr8.1:19550550-19550569 | None:intergenic | 40.0% | |
| TGTTGCTGAGATACAGACAA+AGG | + | chr8.1:19550391-19550410 | None:intergenic | 40.0% | |
| ! | CTCATGATGATGTGACTTCA+CGG | - | chr8.1:19550266-19550285 | MS.gene059926:intron | 40.0% |
| !! | AGATGATAGTGGCTGTGATT+CGG | + | chr8.1:19550569-19550588 | None:intergenic | 40.0% |
| ACAAGATTACCTCTGGTCGA+GGG | + | chr8.1:19550796-19550815 | None:intergenic | 45.0% | |
| AGGGATAAGTATCCTGTGCA+TGG | - | chr8.1:19550127-19550146 | MS.gene059926:CDS | 45.0% | |
| CCACTATCATCTGTCCAACA+AGG | - | chr8.1:19550577-19550596 | MS.gene059926:CDS | 45.0% | |
| CCTTGTTGGACAGATGATAG+TGG | + | chr8.1:19550580-19550599 | None:intergenic | 45.0% | |
| CGTGAAGTCACATCATCATG+AGG | + | chr8.1:19550268-19550287 | None:intergenic | 45.0% | |
| CTAAGGAGGCTTATGAATCG+AGG | - | chr8.1:19550674-19550693 | MS.gene059926:CDS | 45.0% | |
| CTACTCCAACAGCAATAGTG+TGG | - | chr8.1:19550414-19550433 | MS.gene059926:intron | 45.0% | |
| CTCCCTCTACATGGATTCAA+AGG | - | chr8.1:19550450-19550469 | MS.gene059926:intron | 45.0% | |
| CTTTGAATCCATGTAGAGGG+AGG | + | chr8.1:19550452-19550471 | None:intergenic | 45.0% | |
| TCCCATTAGTACGATCACTC+GGG | + | chr8.1:19550845-19550864 | None:intergenic | 45.0% | |
| TCGACCAGAGGTAATCTTGT+CGG | - | chr8.1:19550796-19550815 | MS.gene059926:CDS | 45.0% | |
| ! | CACACTATTGCTGTTGGAGT+AGG | + | chr8.1:19550416-19550435 | None:intergenic | 45.0% |
| ACCAGAGGTAATCTTGTCGG+GGG | - | chr8.1:19550799-19550818 | MS.gene059926:CDS | 50.0% | |
| ATTAGTACGATCACTCGGGG+AGG | + | chr8.1:19550841-19550860 | None:intergenic | 50.0% | |
| CCCATTAGTACGATCACTCG+GGG | + | chr8.1:19550844-19550863 | None:intergenic | 50.0% | |
| CCCCGAGTGATCGTACTAAT+GGG | - | chr8.1:19550841-19550860 | MS.gene059926:CDS | 50.0% | |
| CGACCAGAGGTAATCTTGTC+GGG | - | chr8.1:19550797-19550816 | MS.gene059926:CDS | 50.0% | |
| GACAAGATTACCTCTGGTCG+AGG | + | chr8.1:19550797-19550816 | None:intergenic | 50.0% | |
| GACCAGAGGTAATCTTGTCG+GGG | - | chr8.1:19550798-19550817 | MS.gene059926:CDS | 50.0% | |
| TCACTCGGGGAGGTTAGAAT+TGG | + | chr8.1:19550831-19550850 | None:intergenic | 50.0% | |
| TCCCCGAGTGATCGTACTAA+TGG | - | chr8.1:19550840-19550859 | MS.gene059926:CDS | 50.0% | |
| TCCTCACAACATCCATGCAC+AGG | + | chr8.1:19550142-19550161 | None:intergenic | 50.0% | |
| TCCTGTGCATGGATGTTGTG+AGG | - | chr8.1:19550138-19550157 | MS.gene059926:CDS | 50.0% | |
| !! | GGGCACTGAAGCCACAAATT+AGG | + | chr8.1:19550490-19550509 | None:intergenic | 50.0% |
| ACCCCCGACAAGATTACCTC+TGG | + | chr8.1:19550803-19550822 | None:intergenic | 55.0% | |
| ! | AAAGAGCGCGAGCGCATTGA+AGG | + | chr8.1:19550317-19550336 | None:intergenic | 55.0% |
| CCACAGGTTCCCTCGACCAG+AGG | - | chr8.1:19550784-19550803 | MS.gene059926:CDS | 65.0% | |
| CCTCTGGTCGAGGGAACCTG+TGG | + | chr8.1:19550787-19550806 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 19550097 | 19551087 | 19550097 | ID=MS.gene059926 |
| chr8.1 | mRNA | 19550097 | 19551087 | 19550097 | ID=MS.gene059926.t1;Parent=MS.gene059926 |
| chr8.1 | exon | 19550489 | 19551087 | 19550489 | ID=MS.gene059926.t1.exon1;Parent=MS.gene059926.t1 |
| chr8.1 | CDS | 19550489 | 19551087 | 19550489 | ID=cds.MS.gene059926.t1;Parent=MS.gene059926.t1 |
| chr8.1 | exon | 19550097 | 19550199 | 19550097 | ID=MS.gene059926.t1.exon2;Parent=MS.gene059926.t1 |
| chr8.1 | CDS | 19550097 | 19550199 | 19550097 | ID=cds.MS.gene059926.t1;Parent=MS.gene059926.t1 |
| Gene Sequence |
| Protein sequence |
