Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059951.t1 | KEH15972.1 | 48.5 | 99 | 9 | 1 | 6 | 62 | 57 | 155 | 5.60E-10 | 73.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059951.t1 | Q6S4P4 | 71.2 | 52 | 15 | 0 | 33 | 84 | 134 | 185 | 4.1e-09 | 62.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059951.t1 | A0A072TF27 | 48.5 | 99 | 9 | 1 | 6 | 62 | 57 | 155 | 4.0e-10 | 73.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene059951.t1 | TF | bZIP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059951.t1 | MTR_0405s0060 | 47.525 | 101 | 11 | 1 | 6 | 64 | 57 | 157 | 7.80e-20 | 80.5 |
| MS.gene059951.t1 | MTR_4g072090 | 71.667 | 60 | 16 | 1 | 23 | 81 | 351 | 410 | 8.44e-18 | 76.6 |
| MS.gene059951.t1 | MTR_4g072090 | 71.667 | 60 | 16 | 1 | 23 | 81 | 351 | 410 | 1.12e-17 | 76.6 |
| MS.gene059951.t1 | MTR_2g099050 | 71.154 | 52 | 15 | 0 | 33 | 84 | 223 | 274 | 4.66e-16 | 71.6 |
| MS.gene059951.t1 | MTR_3g467120 | 59.722 | 72 | 23 | 1 | 19 | 84 | 159 | 230 | 3.01e-15 | 69.3 |
| MS.gene059951.t1 | MTR_5g075390 | 59.722 | 72 | 23 | 1 | 19 | 84 | 141 | 212 | 4.96e-15 | 68.6 |
| MS.gene059951.t1 | MTR_6g034945 | 69.231 | 52 | 16 | 0 | 33 | 84 | 153 | 204 | 4.70e-14 | 65.9 |
| MS.gene059951.t1 | MTR_3g094020 | 63.462 | 52 | 19 | 0 | 33 | 84 | 110 | 161 | 1.36e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059951.t1 | AT4G38900 | 68.333 | 60 | 18 | 1 | 23 | 81 | 385 | 444 | 2.97e-16 | 72.4 |
| MS.gene059951.t1 | AT4G38900 | 68.333 | 60 | 18 | 1 | 23 | 81 | 385 | 444 | 2.97e-16 | 72.4 |
| MS.gene059951.t1 | AT1G06850 | 68.519 | 54 | 17 | 0 | 32 | 85 | 149 | 202 | 3.53e-16 | 70.9 |
| MS.gene059951.t1 | AT4G38900 | 84.091 | 44 | 7 | 0 | 38 | 81 | 407 | 450 | 3.72e-16 | 72.4 |
| MS.gene059951.t1 | AT1G06070 | 71.154 | 52 | 15 | 0 | 33 | 84 | 212 | 263 | 3.31e-15 | 69.3 |
| MS.gene059951.t1 | AT1G43700 | 67.308 | 52 | 17 | 0 | 33 | 84 | 196 | 247 | 2.25e-14 | 67.0 |
| MS.gene059951.t1 | AT2G31370 | 71.154 | 52 | 15 | 0 | 33 | 84 | 203 | 254 | 3.44e-14 | 66.6 |
| MS.gene059951.t1 | AT1G06850 | 69.231 | 52 | 16 | 0 | 33 | 84 | 150 | 201 | 5.82e-14 | 65.9 |
| MS.gene059951.t1 | AT2G21230 | 60.938 | 64 | 15 | 1 | 28 | 81 | 357 | 420 | 6.95e-14 | 65.9 |
| MS.gene059951.t1 | AT2G31370 | 71.154 | 52 | 15 | 0 | 33 | 84 | 203 | 254 | 7.35e-14 | 65.5 |
| MS.gene059951.t1 | AT2G31370 | 71.154 | 52 | 15 | 0 | 33 | 84 | 203 | 254 | 7.35e-14 | 65.5 |
| MS.gene059951.t1 | AT2G31370 | 71.154 | 52 | 15 | 0 | 33 | 84 | 203 | 254 | 7.35e-14 | 65.5 |
| MS.gene059951.t1 | AT2G31370 | 71.154 | 52 | 15 | 0 | 33 | 84 | 203 | 254 | 7.35e-14 | 65.5 |
| MS.gene059951.t1 | AT2G31370 | 71.154 | 52 | 15 | 0 | 33 | 84 | 203 | 254 | 7.35e-14 | 65.5 |
| MS.gene059951.t1 | AT2G31370 | 71.154 | 52 | 15 | 0 | 33 | 84 | 203 | 254 | 7.35e-14 | 65.5 |
| MS.gene059951.t1 | AT2G40620 | 67.308 | 52 | 17 | 0 | 33 | 84 | 150 | 201 | 9.04e-14 | 65.5 |
| MS.gene059951.t1 | AT2G21230 | 60.938 | 64 | 15 | 1 | 28 | 81 | 357 | 420 | 2.58e-13 | 64.3 |
| MS.gene059951.t1 | AT2G21230 | 60.938 | 64 | 15 | 1 | 28 | 81 | 357 | 420 | 2.63e-13 | 63.9 |
| MS.gene059951.t1 | AT2G21230 | 60.938 | 64 | 15 | 1 | 28 | 81 | 357 | 420 | 2.63e-13 | 63.9 |
Find 16 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCACAACTTACTTTGTTGC+AGG | 0.290633 | 8.1:-19896054 | MS.gene059951:CDS |
| TTGAACTAGGTTAGGAAAGT+TGG | 0.356724 | 8.1:-19896761 | MS.gene059951:intron |
| AAGGAATGGAATGGATATAA+AGG | 0.396597 | 8.1:-19896734 | MS.gene059951:intron |
| GCGCAAGATGCAATACATTT+CGG | 0.446357 | 8.1:-19896126 | MS.gene059951:CDS |
| TTCTCAGCCTTAGCAATGAA+TGG | 0.462645 | 8.1:+19896585 | None:intergenic |
| AACTAGGTTAGGAAAGTTGG+TGG | 0.467839 | 8.1:-19896758 | MS.gene059951:intron |
| ACAACTTACTTTGTTGCAGG+TGG | 0.538284 | 8.1:-19896051 | MS.gene059951:CDS |
| GGAAAGTTGGTGGAAAGGAA+TGG | 0.542149 | 8.1:-19896748 | MS.gene059951:CDS |
| TTCAAATAATCCTCAAAACA+TGG | 0.555818 | 8.1:+19896004 | None:intergenic |
| CATTTCGGAGCTAGAACAGA+AGG | 0.562989 | 8.1:-19896111 | MS.gene059951:CDS |
| GTTGGTGGAAAGGAATGGAA+TGG | 0.566497 | 8.1:-19896743 | MS.gene059951:CDS |
| GGTTAGGAAAGTTGGTGGAA+AGG | 0.567891 | 8.1:-19896753 | MS.gene059951:CDS |
| TAAGGCTCCATTCATTGCTA+AGG | 0.574542 | 8.1:-19896592 | MS.gene059951:CDS |
| CAACTTACTTTGTTGCAGGT+GGG | 0.580671 | 8.1:-19896050 | MS.gene059951:CDS |
| TAAGGCTGAGAAGTTGAAAG+AGG | 0.582193 | 8.1:-19896574 | MS.gene059951:CDS |
| AAGTTGTGCAGAAAGTGTAG+TGG | 0.665732 | 8.1:+19896067 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTCGTTGAATTTATTTTT+AGG | - | chr8.1:19896065-19896084 | MS.gene059951:CDS | 15.0% |
| !! | TTTAGTTAGTAATGTCAATA+TGG | - | chr8.1:19896664-19896683 | MS.gene059951:intron | 20.0% |
| !!! | ATTTTTTTTTGGGTTATTTC+AGG | - | chr8.1:19896707-19896726 | MS.gene059951:intron | 20.0% |
| !!! | TGACTTGGATATTTTTTTTT+GGG | - | chr8.1:19896697-19896716 | MS.gene059951:intron | 20.0% |
| !!! | TTGACTTGGATATTTTTTTT+TGG | - | chr8.1:19896696-19896715 | MS.gene059951:intron | 20.0% |
| !!! | TTGTGATTTATGTGTATTTT+AGG | - | chr8.1:19896278-19896297 | MS.gene059951:intron | 20.0% |
| !!! | TTTATGTGTATTTTAGGATA+AGG | - | chr8.1:19896284-19896303 | MS.gene059951:intron | 20.0% |
| ! | ATGGATATAAAGGTTGAATT+AGG | - | chr8.1:19896170-19896189 | MS.gene059951:intron | 25.0% |
| ! | TATAAAGGTTGAATTAGGTT+AGG | - | chr8.1:19896175-19896194 | MS.gene059951:intron | 25.0% |
| ! | TTCGGAATTGAAGAAAATTA+TGG | - | chr8.1:19896564-19896583 | MS.gene059951:intron | 25.0% |
| ! | TTTACTTCTCTTATGTTACT+TGG | - | chr8.1:19896417-19896436 | MS.gene059951:intron | 25.0% |
| !! | AATTTTCTTCAATGAAACAG+AGG | + | chr8.1:19896218-19896237 | None:intergenic | 25.0% |
| !! | GAGTTTTCTAACGTAATTTA+CGG | - | chr8.1:19896105-19896124 | MS.gene059951:CDS | 25.0% |
| AAGGAATGGAATGGATATAA+AGG | - | chr8.1:19896160-19896179 | MS.gene059951:CDS | 30.0% | |
| ATTTACGGTGAATTTGAACT+AGG | - | chr8.1:19896120-19896139 | MS.gene059951:CDS | 30.0% | |
| GTTAGTAATGTCAATATGGA+TGG | - | chr8.1:19896668-19896687 | MS.gene059951:intron | 30.0% | |
| ! | TATGGATGGTTAATTTGACT+TGG | - | chr8.1:19896682-19896701 | MS.gene059951:intron | 30.0% |
| !! | AAATCGATTTACCGTTTTTG+TGG | - | chr8.1:19896020-19896039 | MS.gene059951:CDS | 30.0% |
| !! | ATTTCAGGATTTTGAGAAAC+AGG | - | chr8.1:19896722-19896741 | MS.gene059951:intron | 30.0% |
| ACAATGCGTGCTTACAATAT+GGG | - | chr8.1:19896354-19896373 | MS.gene059951:intron | 35.0% | |
| CACAAAACTCACCACAAAAA+CGG | + | chr8.1:19896034-19896053 | None:intergenic | 35.0% | |
| GAAAGTTGCTGAGATTGTTA+TGG | - | chr8.1:19896594-19896613 | MS.gene059951:CDS | 35.0% | |
| TTAGACTGAGAAGAATGCTT+CGG | - | chr8.1:19896482-19896501 | MS.gene059951:intron | 35.0% | |
| TTGAACTAGGTTAGGAAAGT+TGG | - | chr8.1:19896133-19896152 | MS.gene059951:CDS | 35.0% | |
| !! | GATTTTGAGAAACAGGCAAT+CGG | - | chr8.1:19896729-19896748 | MS.gene059951:intron | 35.0% |
| AACTAGGTTAGGAAAGTTGG+TGG | - | chr8.1:19896136-19896155 | MS.gene059951:CDS | 40.0% | |
| ACAACTTACTTTGTTGCAGG+TGG | - | chr8.1:19896843-19896862 | MS.gene059951:intron | 40.0% | |
| CAACTTACTTTGTTGCAGGT+GGG | - | chr8.1:19896844-19896863 | MS.gene059951:intron | 40.0% | |
| CGGTGAATTTGAACTAGGTT+AGG | - | chr8.1:19896125-19896144 | MS.gene059951:CDS | 40.0% | |
| GACAATGCGTGCTTACAATA+TGG | - | chr8.1:19896353-19896372 | MS.gene059951:intron | 40.0% | |
| GCGCAAGATGCAATACATTT+CGG | - | chr8.1:19896768-19896787 | MS.gene059951:intron | 40.0% | |
| TAAGGCTCCATTCATTGCTA+AGG | - | chr8.1:19896302-19896321 | MS.gene059951:intron | 40.0% | |
| TAAGGCTGAGAAGTTGAAAG+AGG | - | chr8.1:19896320-19896339 | MS.gene059951:intron | 40.0% | |
| TGCACAACTTACTTTGTTGC+AGG | - | chr8.1:19896840-19896859 | MS.gene059951:intron | 40.0% | |
| TTCTCAGCCTTAGCAATGAA+TGG | + | chr8.1:19896312-19896331 | None:intergenic | 40.0% | |
| ! | AAGTTGTGCAGAAAGTGTAG+TGG | + | chr8.1:19896830-19896849 | None:intergenic | 40.0% |
| AAGAATGCTTCGGAGGAAGA+AGG | - | chr8.1:19896492-19896511 | MS.gene059951:intron | 45.0% | |
| CATTTCGGAGCTAGAACAGA+AGG | - | chr8.1:19896783-19896802 | MS.gene059951:intron | 45.0% | |
| TTGTTATGGCTGATCCGAAG+CGG | - | chr8.1:19896608-19896627 | MS.gene059951:intron | 45.0% | |
| ! | GTTGGTGGAAAGGAATGGAA+TGG | - | chr8.1:19896151-19896170 | MS.gene059951:CDS | 45.0% |
| !! | GGAAAGTTGGTGGAAAGGAA+TGG | - | chr8.1:19896146-19896165 | MS.gene059951:CDS | 45.0% |
| !! | GGTTAGGAAAGTTGGTGGAA+AGG | - | chr8.1:19896141-19896160 | MS.gene059951:CDS | 45.0% |
| GACTGAGAAGAATGCTTCGG+AGG | - | chr8.1:19896485-19896504 | MS.gene059951:intron | 50.0% | |
| TGTTATGGCTGATCCGAAGC+GGG | - | chr8.1:19896609-19896628 | MS.gene059951:intron | 50.0% | |
| CAACACCTCTTTGCCCGCTT+CGG | + | chr8.1:19896625-19896644 | None:intergenic | 55.0% | |
| CTGATCCGAAGCGGGCAAAG+AGG | - | chr8.1:19896617-19896636 | MS.gene059951:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 19896016 | 19896900 | 19896016 | ID=MS.gene059951 |
| chr8.1 | mRNA | 19896016 | 19896900 | 19896016 | ID=MS.gene059951.t1;Parent=MS.gene059951 |
| chr8.1 | exon | 19896875 | 19896900 | 19896875 | ID=MS.gene059951.t1.exon1;Parent=MS.gene059951.t1 |
| chr8.1 | CDS | 19896875 | 19896900 | 19896875 | ID=cds.MS.gene059951.t1;Parent=MS.gene059951.t1 |
| chr8.1 | exon | 19896735 | 19896774 | 19896735 | ID=MS.gene059951.t1.exon2;Parent=MS.gene059951.t1 |
| chr8.1 | CDS | 19896735 | 19896774 | 19896735 | ID=cds.MS.gene059951.t1;Parent=MS.gene059951.t1 |
| chr8.1 | exon | 19896573 | 19896616 | 19896573 | ID=MS.gene059951.t1.exon3;Parent=MS.gene059951.t1 |
| chr8.1 | CDS | 19896573 | 19896616 | 19896573 | ID=cds.MS.gene059951.t1;Parent=MS.gene059951.t1 |
| chr8.1 | exon | 19896016 | 19896187 | 19896016 | ID=MS.gene059951.t1.exon4;Parent=MS.gene059951.t1 |
| chr8.1 | CDS | 19896016 | 19896187 | 19896016 | ID=cds.MS.gene059951.t1;Parent=MS.gene059951.t1 |
| Gene Sequence |
| Protein sequence |
