Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059981.t1 | XP_024634877.1 | 91.4 | 175 | 15 | 0 | 1 | 175 | 38 | 212 | 7.70E-88 | 333.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059981.t1 | Q9SWG3 | 29.7 | 155 | 92 | 4 | 22 | 171 | 46 | 188 | 1.7e-06 | 54.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059981.t1 | A0A072VE81 | 68.1 | 166 | 52 | 1 | 14 | 179 | 11 | 175 | 3.1e-54 | 221.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene059981.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059981.t1 | MTR_1g013060 | 68.072 | 166 | 52 | 1 | 14 | 179 | 11 | 175 | 2.55e-75 | 226 |
| MS.gene059981.t1 | MTR_6g060410 | 80.741 | 135 | 15 | 2 | 1 | 135 | 61 | 184 | 8.94e-75 | 223 |
| MS.gene059981.t1 | MTR_4g080290 | 92.381 | 105 | 8 | 0 | 1 | 105 | 1 | 105 | 2.12e-69 | 206 |
| MS.gene059981.t1 | MTR_4g067290 | 37.302 | 126 | 67 | 4 | 11 | 134 | 41 | 156 | 1.17e-18 | 80.5 |
| MS.gene059981.t1 | MTR_4g067290 | 37.302 | 126 | 67 | 4 | 11 | 134 | 41 | 156 | 1.34e-18 | 80.5 |
| MS.gene059981.t1 | MTR_4g067290 | 37.302 | 126 | 67 | 4 | 11 | 134 | 61 | 176 | 2.11e-18 | 80.1 |
| MS.gene059981.t1 | MTR_4g067290 | 37.302 | 126 | 67 | 4 | 11 | 134 | 59 | 174 | 2.15e-18 | 80.1 |
| MS.gene059981.t1 | MTR_4g067290 | 37.302 | 126 | 67 | 4 | 11 | 134 | 61 | 176 | 2.22e-18 | 80.1 |
| MS.gene059981.t1 | MTR_4g067290 | 37.302 | 126 | 67 | 4 | 11 | 134 | 101 | 216 | 2.75e-18 | 80.5 |
| MS.gene059981.t1 | MTR_2g098920 | 34.559 | 136 | 78 | 3 | 1 | 134 | 54 | 180 | 1.61e-15 | 72.8 |
| MS.gene059981.t1 | MTR_2g098880 | 33.333 | 135 | 74 | 3 | 23 | 151 | 57 | 181 | 2.44e-13 | 66.6 |
| MS.gene059981.t1 | MTR_2g098880 | 33.333 | 135 | 74 | 3 | 23 | 151 | 69 | 193 | 3.17e-13 | 66.2 |
| MS.gene059981.t1 | MTR_6g051425 | 29.221 | 154 | 100 | 3 | 26 | 174 | 13 | 162 | 6.80e-13 | 65.9 |
| MS.gene059981.t1 | MTR_0853s0010 | 29.630 | 135 | 88 | 2 | 25 | 158 | 23 | 151 | 2.91e-12 | 62.0 |
| MS.gene059981.t1 | MTR_0558s0020 | 31.646 | 158 | 91 | 6 | 32 | 174 | 41 | 196 | 2.16e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059981.t1 | AT3G59470 | 37.838 | 111 | 58 | 3 | 26 | 134 | 69 | 170 | 1.20e-14 | 70.1 |
| MS.gene059981.t1 | AT3G59470 | 37.838 | 111 | 58 | 3 | 26 | 134 | 69 | 170 | 1.20e-14 | 70.1 |
| MS.gene059981.t1 | AT3G59470 | 37.838 | 111 | 58 | 3 | 26 | 134 | 81 | 182 | 1.61e-14 | 70.1 |
| MS.gene059981.t1 | AT4G38180 | 32.258 | 155 | 94 | 3 | 24 | 174 | 70 | 217 | 5.51e-14 | 69.7 |
| MS.gene059981.t1 | AT3G07500 | 32.432 | 111 | 67 | 2 | 25 | 134 | 31 | 134 | 1.39e-13 | 66.6 |
Find 21 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTATCATCCGTCTTATT+TGG | 0.253463 | 6.3:+41063209 | None:intergenic |
| TTTAATTCATCATCGCAATT+TGG | 0.259676 | 6.3:+41063395 | None:intergenic |
| AGTTCACAAAAGCTTATTAC+AGG | 0.312085 | 6.3:-41062997 | MS.gene059981:CDS |
| TCAAATACTTGTCCAATCTT+AGG | 0.341635 | 6.3:+41063371 | None:intergenic |
| CTTAGAAACGGTCTCTTCAT+AGG | 0.411850 | 6.3:+41063041 | None:intergenic |
| CTATGAAGAGACCGTTTCTA+AGG | 0.416138 | 6.3:-41063040 | MS.gene059981:CDS |
| GATGAATTAAAGCCTAAGAT+TGG | 0.441758 | 6.3:-41063383 | MS.gene059981:CDS |
| GCATGTAGTAGAGCAAATAT+TGG | 0.447678 | 6.3:-41062975 | MS.gene059981:CDS |
| GCCAAATAAGACGGATGATA+AGG | 0.447708 | 6.3:-41063210 | MS.gene059981:CDS |
| TCTTGCATGCCTGTAATAAG+TGG | 0.485501 | 6.3:+41062937 | None:intergenic |
| CTGTCAAGGTTAGAAGAAGA+AGG | 0.491761 | 6.3:-41063172 | MS.gene059981:CDS |
| TTCCTTGCAGACCTTAGAAA+CGG | 0.510667 | 6.3:+41063029 | None:intergenic |
| AGAAGGGCTTTAACAAGAGA+AGG | 0.550276 | 6.3:-41063155 | MS.gene059981:CDS |
| GGGTTATTTGCCAAATAAGA+CGG | 0.573634 | 6.3:-41063219 | MS.gene059981:CDS |
| GTGAAGCAAGTGGATGTGTA+TGG | 0.586364 | 6.3:+41063063 | None:intergenic |
| GCAAAGTGAATCTACTGTCA+AGG | 0.595204 | 6.3:-41063186 | MS.gene059981:CDS |
| GCATGCCTGTAATAAGTGGA+AGG | 0.595295 | 6.3:+41062941 | None:intergenic |
| TGTCAAGGTTAGAAGAAGAA+GGG | 0.596214 | 6.3:-41063171 | MS.gene059981:CDS |
| GACCGTTTCTAAGGTCTGCA+AGG | 0.602665 | 6.3:-41063031 | MS.gene059981:CDS |
| GTATTTGATACATTAGCAGA+AGG | 0.616435 | 6.3:-41063356 | MS.gene059981:CDS |
| CTCTTCATAGGTGAAGCAAG+TGG | 0.639442 | 6.3:+41063053 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATATATTAATATATAAAG+TGG | + | chr6.3:41063119-41063138 | None:intergenic | 10.0% |
| !! | TCTTCAATAAAATAATATAA+AGG | + | chr6.3:41063516-41063535 | None:intergenic | 10.0% |
| !! | AAAAAAAACAACTATCTCTT+AGG | + | chr6.3:41062995-41063014 | None:intergenic | 20.0% |
| !! | AAACAATTATAATATACCTG+AGG | + | chr6.3:41062956-41062975 | None:intergenic | 20.0% |
| !! | AATAGAACCAACAAAAAAAT+AGG | + | chr6.3:41063020-41063039 | None:intergenic | 20.0% |
| !! | AGGATTAAACAAAGAATTAA+AGG | + | chr6.3:41063475-41063494 | None:intergenic | 20.0% |
| !! | ATAGAACCAACAAAAAAATA+GGG | + | chr6.3:41063019-41063038 | None:intergenic | 20.0% |
| !! | GGAAAACAGAATAAATAAAA+AGG | + | chr6.3:41063495-41063514 | None:intergenic | 20.0% |
| !!! | ATATATACATGATTAGTTCA+AGG | + | chr6.3:41063244-41063263 | None:intergenic | 20.0% |
| !!! | TATATACATGATTAGTTCAA+GGG | + | chr6.3:41063243-41063262 | None:intergenic | 20.0% |
| !!! | TTATGTTTTGTTCTTTTTTG+AGG | - | chr6.3:41063093-41063112 | MS.gene059981:CDS | 20.0% |
| !!! | TTTTTTCCCTATTTTTTTGT+TGG | - | chr6.3:41063010-41063029 | MS.gene059981:CDS | 20.0% |
| ! | ACAAGAAAAAACATGAATGT+AGG | - | chr6.3:41063179-41063198 | MS.gene059981:CDS | 25.0% |
| ! | TTATTATCATTATGAGCACA+TGG | - | chr6.3:41063336-41063355 | MS.gene059981:CDS | 25.0% |
| ! | TTTAATTCATCATCGCAATT+TGG | + | chr6.3:41063575-41063594 | None:intergenic | 25.0% |
| !! | TCATCGACTATAGATAAAAA+CGG | - | chr6.3:41063689-41063708 | MS.gene059981:intron | 25.0% |
| !!! | ATGCAAATGTTGTTTTCAAA+TGG | - | chr6.3:41063840-41063859 | MS.gene059981:intron | 25.0% |
| !!! | TAATGTTGACTTCTAATTTG+TGG | - | chr6.3:41063392-41063411 | MS.gene059981:CDS | 25.0% |
| !!! | TGCAAATGTTGTTTTCAAAT+GGG | - | chr6.3:41063841-41063860 | MS.gene059981:intron | 25.0% |
| AAAAACCTTCCACTTATTAC+AGG | - | chr6.3:41064021-41064040 | MS.gene059981:intron | 30.0% | |
| AGTTCACAAAAGCTTATTAC+AGG | - | chr6.3:41063970-41063989 | MS.gene059981:intron | 30.0% | |
| GATGAATTAAAGCCTAAGAT+TGG | - | chr6.3:41063584-41063603 | MS.gene059981:intron | 30.0% | |
| GTATTTGATACATTAGCAGA+AGG | - | chr6.3:41063611-41063630 | MS.gene059981:intron | 30.0% | |
| TCAAATACTTGTCCAATCTT+AGG | + | chr6.3:41063599-41063618 | None:intergenic | 30.0% | |
| TCTCTAATATGCAGAAAGAT+TGG | + | chr6.3:41063212-41063231 | None:intergenic | 30.0% | |
| TCTTGTTCATTAATGTCTAC+AGG | + | chr6.3:41063165-41063184 | None:intergenic | 30.0% | |
| !! | ATGAGATTGACTTTCACTTT+TGG | + | chr6.3:41063284-41063303 | None:intergenic | 30.0% |
| !!! | AAATGTTGTTTTCAAATGGG+TGG | - | chr6.3:41063844-41063863 | MS.gene059981:intron | 30.0% |
| !!! | AACTAGTTTTCAAATTCCTC+AGG | - | chr6.3:41062937-41062956 | MS.gene059981:CDS | 30.0% |
| !!! | AATGTTGTTTTCAAATGGGT+GGG | - | chr6.3:41063845-41063864 | MS.gene059981:intron | 30.0% |
| !!! | TTTTTAAGATTGTTGTCCAG+TGG | - | chr6.3:41063439-41063458 | MS.gene059981:intron | 30.0% |
| GCATGTAGTAGAGCAAATAT+TGG | - | chr6.3:41063992-41064011 | MS.gene059981:intron | 35.0% | |
| GGGTTATTTGCCAAATAAGA+CGG | - | chr6.3:41063748-41063767 | MS.gene059981:intron | 35.0% | |
| GTTCATTAATGTCTACAGGT+TGG | + | chr6.3:41063161-41063180 | None:intergenic | 35.0% | |
| TGTCAAGGTTAGAAGAAGAA+GGG | - | chr6.3:41063796-41063815 | MS.gene059981:intron | 35.0% | |
| ! | GAAGCACGAGATAAGTTTTA+TGG | + | chr6.3:41063368-41063387 | None:intergenic | 35.0% |
| ! | TCCTTATCATCCGTCTTATT+TGG | + | chr6.3:41063761-41063780 | None:intergenic | 35.0% |
| !! | ACATTTTGTTTGCTCGAAAG+AGG | - | chr6.3:41063727-41063746 | MS.gene059981:intron | 35.0% |
| !! | CACGAGATAAGTTTTATGGT+TGG | + | chr6.3:41063364-41063383 | None:intergenic | 35.0% |
| !! | CATTTTGTTTGCTCGAAAGA+GGG | - | chr6.3:41063728-41063747 | MS.gene059981:intron | 35.0% |
| !! | TTGTTGGTTCTATTGTTGTC+CGG | - | chr6.3:41063026-41063045 | MS.gene059981:CDS | 35.0% |
| !!! | ATGTTGTTTTCAAATGGGTG+GGG | - | chr6.3:41063846-41063865 | MS.gene059981:intron | 35.0% |
| CAACAAGCAAATAGAACCAC+CGG | + | chr6.3:41063048-41063067 | None:intergenic | 40.0% | |
| CTATGAAGAGACCGTTTCTA+AGG | - | chr6.3:41063927-41063946 | MS.gene059981:intron | 40.0% | |
| CTGTCAAGGTTAGAAGAAGA+AGG | - | chr6.3:41063795-41063814 | MS.gene059981:intron | 40.0% | |
| CTTAGAAACGGTCTCTTCAT+AGG | + | chr6.3:41063929-41063948 | None:intergenic | 40.0% | |
| GAAAAATACGCAGTCTCTGT+CGG | - | chr6.3:41063647-41063666 | MS.gene059981:intron | 40.0% | |
| GCAAAGTGAATCTACTGTCA+AGG | - | chr6.3:41063781-41063800 | MS.gene059981:intron | 40.0% | |
| GCCAAATAAGACGGATGATA+AGG | - | chr6.3:41063757-41063776 | MS.gene059981:intron | 40.0% | |
| TAAAGGATCATCAGAACCAC+TGG | + | chr6.3:41063458-41063477 | None:intergenic | 40.0% | |
| TCTTGCATGCCTGTAATAAG+TGG | + | chr6.3:41064033-41064052 | None:intergenic | 40.0% | |
| TTCCTTGCAGACCTTAGAAA+CGG | + | chr6.3:41063941-41063960 | None:intergenic | 40.0% | |
| ! | AGAAGGGCTTTAACAAGAGA+AGG | - | chr6.3:41063812-41063831 | MS.gene059981:intron | 40.0% |
| !!! | TGTTGTTTTCAAATGGGTGG+GGG | - | chr6.3:41063847-41063866 | MS.gene059981:intron | 40.0% |
| !!! | TTTCACTTTTGGCTTCTCAC+TGG | + | chr6.3:41063273-41063292 | None:intergenic | 40.0% |
| ATAAAAACGGCGTGAAGCGT+TGG | - | chr6.3:41063702-41063721 | MS.gene059981:intron | 45.0% | |
| CTCTTCATAGGTGAAGCAAG+TGG | + | chr6.3:41063917-41063936 | None:intergenic | 45.0% | |
| GCATGCCTGTAATAAGTGGA+AGG | + | chr6.3:41064029-41064048 | None:intergenic | 45.0% | |
| GTGAAGCAAGTGGATGTGTA+TGG | + | chr6.3:41063907-41063926 | None:intergenic | 45.0% | |
| ! | CGGTGGTTCTATTTGCTTGT+TGG | - | chr6.3:41063046-41063065 | MS.gene059981:CDS | 45.0% |
| !! | TTGGTTCTATTGTTGTCCGG+TGG | - | chr6.3:41063029-41063048 | MS.gene059981:CDS | 45.0% |
| !! | TTTATATATTAATATATGTT+AGG | - | chr6.3:41063120-41063139 | MS.gene059981:CDS | 5.0% |
| !!! | ATATTTATTTTGTTAATTTT+AGG | - | chr6.3:41063535-41063554 | MS.gene059981:intron | 5.0% |
| ! | GTTTTCAAATGGGTGGGGGA+AGG | - | chr6.3:41063851-41063870 | MS.gene059981:intron | 50.0% |
| !! | GACCGTTTCTAAGGTCTGCA+AGG | - | chr6.3:41063936-41063955 | MS.gene059981:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 41062920 | 41064069 | 41062920 | ID=MS.gene059981 |
| chr6.3 | mRNA | 41062920 | 41064069 | 41062920 | ID=MS.gene059981.t1;Parent=MS.gene059981 |
| chr6.3 | exon | 41064031 | 41064069 | 41064031 | ID=MS.gene059981.t1.exon1;Parent=MS.gene059981.t1 |
| chr6.3 | CDS | 41064031 | 41064069 | 41064031 | ID=cds.MS.gene059981.t1;Parent=MS.gene059981.t1 |
| chr6.3 | exon | 41062920 | 41063432 | 41062920 | ID=MS.gene059981.t1.exon2;Parent=MS.gene059981.t1 |
| chr6.3 | CDS | 41062920 | 41063432 | 41062920 | ID=cds.MS.gene059981.t1;Parent=MS.gene059981.t1 |
| Gene Sequence |
| Protein sequence |
