AlfalfaGEDB | MS.gene060262

M. sativa cultivar XinJiangDaYe / MS.gene060262

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060262.t1	AES98839.2	86.9	84	11	0	1	84	1	84	2.30E-34	154.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060262.t1	Q9SRH9	42.9	63	34	1	12	74	58	118	2.0e-07	56.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060262.t1	G7JZR3	86.9	84	11	0	1	84	1	84	1.6e-34	154.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene060262.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060262.t1	MTR_5g073430	86.905	84	11	0	1	84	1	84	4.05e-50	153
MS.gene060262.t1	MTR_7g083950	62.025	79	30	0	1	79	2	80	1.60e-27	103
MS.gene060262.t1	MTR_1g105960	48.529	68	31	3	12	79	2	65	3.62e-12	57.4
MS.gene060262.t1	MTR_3g006150	49.153	59	30	0	16	74	94	152	1.72e-11	56.6
MS.gene060262.t1	MTR_8g062700	47.059	68	32	3	12	79	43	106	2.35e-11	58.2
MS.gene060262.t1	MTR_8g062700	47.059	68	32	3	12	79	43	106	2.35e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 23 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGCATCAGGGTTTATTATT+CGG	0.287310	5.4:-60248232	MS.gene060262:CDS
TGCACTGGAGTATCTTCTTA+AGG	0.379025	5.4:-60248383	MS.gene060262:CDS
TGGGATGCTTCAAAGGCATC+AGG	0.403391	5.4:-60248245	MS.gene060262:CDS
GATACTCCAGTGCATGTTCA+TGG	0.422585	5.4:+60248392	None:intergenic
GCATCCCACTAACTCTTGAT+TGG	0.437559	5.4:+60248260	None:intergenic
GCACTGGAGTATCTTCTTAA+GGG	0.471379	5.4:-60248382	MS.gene060262:CDS
AAAACTCACAAGAGATTACA+AGG	0.479131	5.4:-60248308	MS.gene060262:CDS
ACCTTCCTCATCATGCCAGC+TGG	0.479240	5.4:-60248202	MS.gene060262:CDS
AAGGCTGGAAGAATTGATAT+AGG	0.488405	5.4:-60248289	MS.gene060262:CDS
CTCACAAGAGATTACAAGGC+TGG	0.498781	5.4:-60248304	MS.gene060262:CDS
GCTGGTTTGAACTCTTTCCA+TGG	0.521060	5.4:-60248184	None:intergenic
GAGTTAGTGGGATGCTTCAA+AGG	0.526784	5.4:-60248252	MS.gene060262:CDS
GGCATCCAATCAAGAGTTAG+TGG	0.541734	5.4:-60248265	MS.gene060262:CDS
TTATTGCCATGAACATGCAC+TGG	0.547671	5.4:-60248398	MS.gene060262:CDS
TGGAAAGAGTTCAAACCAGC+TGG	0.553560	5.4:+60248187	None:intergenic
TGTTCATGGCAATAATTTCG+AGG	0.557832	5.4:+60248406	None:intergenic
GGGATGCTTCAAAGGCATCA+GGG	0.590682	5.4:-60248244	MS.gene060262:CDS
TCAAACCAGCTGGCATGATG+AGG	0.594872	5.4:+60248197	None:intergenic
GTTCATGGCAATAATTTCGA+GGG	0.608298	5.4:+60248407	None:intergenic
ACCAGCTGGCATGATGAGGA+AGG	0.612834	5.4:+60248201	None:intergenic
AGGGAGTGAAACTTGCATCT+CGG	0.621205	5.4:-60248363	MS.gene060262:CDS
GCTGGAAGAATTGATATAGG+AGG	0.622426	5.4:-60248286	MS.gene060262:CDS
GCATCCAATCAAGAGTTAGT+GGG	0.632249	5.4:-60248264	MS.gene060262:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAACTCACAAGAGATTACA+AGG	-	chr5.4:60248292-60248311	MS.gene060262:CDS	30.0%
	AAGGCTGGAAGAATTGATAT+AGG	-	chr5.4:60248311-60248330	MS.gene060262:CDS	35.0%
	AGGCATCAGGGTTTATTATT+CGG	-	chr5.4:60248368-60248387	MS.gene060262:CDS	35.0%
	GTTCATGGCAATAATTTCGA+GGG	+	chr5.4:60248196-60248215	None:intergenic	35.0%
	TGTTCATGGCAATAATTTCG+AGG	+	chr5.4:60248197-60248216	None:intergenic	35.0%
	GCATCCAATCAAGAGTTAGT+GGG	-	chr5.4:60248336-60248355	MS.gene060262:CDS	40.0%
	GCTGGAAGAATTGATATAGG+AGG	-	chr5.4:60248314-60248333	MS.gene060262:CDS	40.0%
	TTATTGCCATGAACATGCAC+TGG	-	chr5.4:60248202-60248221	MS.gene060262:CDS	40.0%
!	GCACTGGAGTATCTTCTTAA+GGG	-	chr5.4:60248218-60248237	MS.gene060262:CDS	40.0%
!	TGCACTGGAGTATCTTCTTA+AGG	-	chr5.4:60248217-60248236	MS.gene060262:CDS	40.0%
	AGGGAGTGAAACTTGCATCT+CGG	-	chr5.4:60248237-60248256	MS.gene060262:CDS	45.0%
	CTCACAAGAGATTACAAGGC+TGG	-	chr5.4:60248296-60248315	MS.gene060262:CDS	45.0%
	GAGTTAGTGGGATGCTTCAA+AGG	-	chr5.4:60248348-60248367	MS.gene060262:CDS	45.0%
	GATACTCCAGTGCATGTTCA+TGG	+	chr5.4:60248211-60248230	None:intergenic	45.0%
	GCATCCCACTAACTCTTGAT+TGG	+	chr5.4:60248343-60248362	None:intergenic	45.0%
	GGCATCCAATCAAGAGTTAG+TGG	-	chr5.4:60248335-60248354	MS.gene060262:CDS	45.0%
	TGGAAAGAGTTCAAACCAGC+TGG	+	chr5.4:60248416-60248435	None:intergenic	45.0%
	TCAAACCAGCTGGCATGATG+AGG	+	chr5.4:60248406-60248425	None:intergenic	50.0%
!!	GGGATGCTTCAAAGGCATCA+GGG	-	chr5.4:60248356-60248375	MS.gene060262:CDS	50.0%
!!	TGGGATGCTTCAAAGGCATC+AGG	-	chr5.4:60248355-60248374	MS.gene060262:CDS	50.0%
	ACCAGCTGGCATGATGAGGA+AGG	+	chr5.4:60248402-60248421	None:intergenic	55.0%
	ACCTTCCTCATCATGCCAGC+TGG	-	chr5.4:60248398-60248417	MS.gene060262:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	60248185	60248437	60248185	ID=MS.gene060262
chr5.4	mRNA	60248185	60248437	60248185	ID=MS.gene060262.t1;Parent=MS.gene060262
chr5.4	exon	60248185	60248437	60248185	ID=MS.gene060262.t1.exon1;Parent=MS.gene060262.t1
chr5.4	CDS	60248185	60248437	60248185	ID=cds.MS.gene060262.t1;Parent=MS.gene060262.t1

Gene Sequence	>MS.gene060262 ATGAACATCCCTCGAAATTATTGCCATGAACATGCACTGGAGTATCTTCTTAAGGGAGTGAAACTTGCATCTCGGTACGATAACAGCATAAAGTACGATGAGTTTTTAAAACTCACAAGAGATTACAAGGCTGGAAGAATTGATATAGGAGGCATCCAATCAAGAGTTAGTGGGATGCTTCAAAGGCATCAGGGTTTATTATTCGGATTCAACACCTTCCTCATCATGCCAGCTGGTTTGAACTCTTTCCATG
Protein sequence	>MS.gene060262.t1 MNIPRNYCHEHALEYLLKGVKLASRYDNSIKYDEFLKLTRDYKAGRIDIGGIQSRVSGMLQRHQGLLFGFNTFLIMPAGLNSFH