AlfalfaGEDB | MS.gene060263

M. sativa cultivar XinJiangDaYe / MS.gene060263

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060263.t1	AES98839.2	88.5	78	9	0	1	78	1	78	3.30E-32	147.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060263.t1	Q9SRH9	42.9	63	34	1	12	74	58	118	1.9e-07	56.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060263.t1	G7JZR3	88.5	78	9	0	1	78	1	78	2.4e-32	147.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene060263.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene060263.t1	MTR_5g073430	88.462	78	9	0	1	78	1	78	1.83e-47	146
MS.gene060263.t1	MTR_7g083950	61.538	78	30	0	1	78	2	79	1.49e-26	100
MS.gene060263.t1	MTR_1g105960	49.206	63	30	2	12	74	2	62	5.17e-12	56.6
MS.gene060263.t1	MTR_3g006150	49.153	59	30	0	16	74	94	152	1.28e-11	57.0
MS.gene060263.t1	MTR_8g062700	47.619	63	31	2	12	74	43	103	2.25e-11	57.8
MS.gene060263.t1	MTR_8g062700	47.619	63	31	2	12	74	43	103	2.25e-11	57.8
MS.gene060263.t1	MTR_3g006185	47.458	59	31	0	16	74	102	160	9.16e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 21 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGCATCAGGGTTTATTATT+CGG	0.287310	5.4:-60285232	MS.gene060263:CDS
TGCACTGGAGTATCTTCTTA+AGG	0.379025	5.4:-60285383	MS.gene060263:CDS
TGGGATGCTTCAAAGGCATC+AGG	0.403391	5.4:-60285245	MS.gene060263:CDS
GATACTCCAGTGCATGTTCA+TGG	0.422585	5.4:+60285392	None:intergenic
GCATCCCACTAACTCTTGAT+TGG	0.437559	5.4:+60285260	None:intergenic
GCACTGGAGTATCTTCTTAA+GGG	0.471379	5.4:-60285382	MS.gene060263:CDS
AAAACTCACAAGAGATTACA+AGG	0.479131	5.4:-60285308	MS.gene060263:CDS
ACCTTCCTCATCATGCCAGC+TGG	0.479240	5.4:-60285202	None:intergenic
AAGGCTGGAAGAATTGATAT+AGG	0.488405	5.4:-60285289	MS.gene060263:CDS
CTCACAAGAGATTACAAGGC+TGG	0.498781	5.4:-60285304	MS.gene060263:CDS
GAGTTAGTGGGATGCTTCAA+AGG	0.526784	5.4:-60285252	MS.gene060263:CDS
GGCATCCAATCAAGAGTTAG+TGG	0.541734	5.4:-60285265	MS.gene060263:CDS
TTATTGCCATGAACATGCAC+TGG	0.547671	5.4:-60285398	MS.gene060263:CDS
TGTTCATGGCAATAATTTCG+AGG	0.557832	5.4:+60285406	None:intergenic
GGGATGCTTCAAAGGCATCA+GGG	0.590682	5.4:-60285244	MS.gene060263:CDS
TCAAACCAGCTGGCATGATG+AGG	0.594872	5.4:+60285197	None:intergenic
GTTCATGGCAATAATTTCGA+GGG	0.608298	5.4:+60285407	None:intergenic
ACCAGCTGGCATGATGAGGA+AGG	0.612834	5.4:+60285201	None:intergenic
AGGGAGTGAAACTTGCATCT+CGG	0.621205	5.4:-60285363	MS.gene060263:CDS
GCTGGAAGAATTGATATAGG+AGG	0.622426	5.4:-60285286	MS.gene060263:CDS
GCATCCAATCAAGAGTTAGT+GGG	0.632249	5.4:-60285264	MS.gene060263:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAACTCACAAGAGATTACA+AGG	-	chr5.4:60285310-60285329	MS.gene060263:CDS	30.0%
	AAGGCTGGAAGAATTGATAT+AGG	-	chr5.4:60285329-60285348	MS.gene060263:CDS	35.0%
	AGGCATCAGGGTTTATTATT+CGG	-	chr5.4:60285386-60285405	MS.gene060263:CDS	35.0%
	GTTCATGGCAATAATTTCGA+GGG	+	chr5.4:60285214-60285233	None:intergenic	35.0%
	TGTTCATGGCAATAATTTCG+AGG	+	chr5.4:60285215-60285234	None:intergenic	35.0%
	GCATCCAATCAAGAGTTAGT+GGG	-	chr5.4:60285354-60285373	MS.gene060263:CDS	40.0%
	GCTGGAAGAATTGATATAGG+AGG	-	chr5.4:60285332-60285351	MS.gene060263:CDS	40.0%
	TTATTGCCATGAACATGCAC+TGG	-	chr5.4:60285220-60285239	MS.gene060263:CDS	40.0%
!	GCACTGGAGTATCTTCTTAA+GGG	-	chr5.4:60285236-60285255	MS.gene060263:CDS	40.0%
!	TGCACTGGAGTATCTTCTTA+AGG	-	chr5.4:60285235-60285254	MS.gene060263:CDS	40.0%
	AGGGAGTGAAACTTGCATCT+CGG	-	chr5.4:60285255-60285274	MS.gene060263:CDS	45.0%
	CTCACAAGAGATTACAAGGC+TGG	-	chr5.4:60285314-60285333	MS.gene060263:CDS	45.0%
	GAGTTAGTGGGATGCTTCAA+AGG	-	chr5.4:60285366-60285385	MS.gene060263:CDS	45.0%
	GATACTCCAGTGCATGTTCA+TGG	+	chr5.4:60285229-60285248	None:intergenic	45.0%
	GCATCCCACTAACTCTTGAT+TGG	+	chr5.4:60285361-60285380	None:intergenic	45.0%
	GGCATCCAATCAAGAGTTAG+TGG	-	chr5.4:60285353-60285372	MS.gene060263:CDS	45.0%
!!	GGGATGCTTCAAAGGCATCA+GGG	-	chr5.4:60285374-60285393	MS.gene060263:CDS	50.0%
!!	TGGGATGCTTCAAAGGCATC+AGG	-	chr5.4:60285373-60285392	MS.gene060263:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	60285203	60285437	60285203	ID=MS.gene060263
chr5.4	mRNA	60285203	60285437	60285203	ID=MS.gene060263.t1;Parent=MS.gene060263
chr5.4	exon	60285203	60285437	60285203	ID=MS.gene060263.t1.exon1;Parent=MS.gene060263.t1
chr5.4	CDS	60285203	60285437	60285203	ID=cds.MS.gene060263.t1;Parent=MS.gene060263.t1

Gene Sequence	>MS.gene060263 ATGAACATCCCTCGAAATTATTGCCATGAACATGCACTGGAGTATCTTCTTAAGGGAGTGAAACTTGCATCTCGGTACGATAACAGCATAAAGTACGATGAGTTTTTAAAACTCACAAGAGATTACAAGGCTGGAAGAATTGATATAGGAGGCATCCAATCAAGAGTTAGTGGGATGCTTCAAAGGCATCAGGGTTTATTATTCGGATTCAACACCTTCCTCATCATGCCAGCTG
Protein sequence	>MS.gene060263.t1 MNIPRNYCHEHALEYLLKGVKLASRYDNSIKYDEFLKLTRDYKAGRIDIGGIQSRVSGMLQRHQGLLFGFNTFLIMPA