AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060651

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060651.t1 MTR_2g078200 89.667 300 23 4 1 299 1 293 7.87e-153 429
MS.gene060651.t1 MTR_8g463380 41.422 408 118 13 3 300 37 433 6.01e-75 236
MS.gene060651.t1 MTR_2g090960 38.529 340 135 11 2 291 6 321 1.72e-58 190
MS.gene060651.t1 MTR_4g079580 36.254 331 143 9 3 281 5 319 4.07e-49 166
MS.gene060651.t1 MTR_7g015010 62.162 74 28 0 14 87 51 124 8.29e-25 102
MS.gene060651.t1 MTR_6g015350 63.235 68 25 0 14 81 43 110 4.25e-21 92.0
MS.gene060651.t1 MTR_4g109660 58.824 68 28 0 14 81 50 117 1.63e-20 89.7
MS.gene060651.t1 MTR_3g026050 47.423 97 47 2 14 109 51 144 2.72e-20 90.5
MS.gene060651.t1 MTR_1g103380 57.377 61 26 0 19 79 200 260 1.12e-16 80.5
MS.gene060651.t1 MTR_6g017055 58.333 60 25 0 20 79 126 185 3.96e-16 78.6
MS.gene060651.t1 MTR_7g018500 58.621 58 24 0 22 79 122 179 8.21e-16 77.4
MS.gene060651.t1 MTR_4g111935 53.731 67 30 1 14 79 125 191 5.73e-15 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060651.t1 AT1G53230 42.400 375 122 16 4 300 33 391 4.30e-67 214
MS.gene060651.t1 AT1G53230 42.400 375 122 16 4 300 33 391 4.30e-67 214
MS.gene060651.t1 AT2G31070 85.526 76 11 0 3 78 12 87 1.44e-39 142
MS.gene060651.t1 AT4G18390 50.000 100 42 2 2 101 29 120 1.18e-23 99.8
MS.gene060651.t1 AT4G18390 50.000 100 42 2 2 101 29 120 1.18e-23 99.8
MS.gene060651.t1 AT3G02150 63.768 69 25 0 14 82 68 136 3.55e-23 96.7
MS.gene060651.t1 AT3G02150 57.317 82 33 1 14 93 68 149 3.94e-23 97.8
MS.gene060651.t1 AT1G30210 58.333 84 31 1 14 97 44 123 1.75e-22 95.9
MS.gene060651.t1 AT1G30210 58.333 84 31 1 14 97 44 123 1.75e-22 95.9
MS.gene060651.t1 AT5G60970 58.824 68 28 0 14 81 57 124 8.51e-21 91.7
MS.gene060651.t1 AT5G08070 53.571 84 32 2 2 83 20 98 3.64e-20 87.8
MS.gene060651.t1 AT5G08070 53.571 84 32 2 2 83 20 98 3.64e-20 87.8
MS.gene060651.t1 AT1G68800 57.143 63 27 0 16 78 108 170 8.24e-20 88.6
MS.gene060651.t1 AT1G68800 57.143 63 27 0 16 78 108 170 8.47e-20 88.6
MS.gene060651.t1 AT3G15030 45.205 146 61 4 168 300 281 420 1.49e-18 85.5
MS.gene060651.t1 AT3G15030 45.205 146 61 4 168 300 281 420 1.49e-18 85.5
MS.gene060651.t1 AT3G15030 45.205 146 61 4 168 300 281 420 1.49e-18 85.5
MS.gene060651.t1 AT3G15030 45.205 146 61 4 168 300 281 420 1.49e-18 85.5
MS.gene060651.t1 AT1G67260 54.545 66 30 0 15 80 82 147 1.68e-17 82.0
MS.gene060651.t1 AT1G67260 54.545 66 30 0 15 80 82 147 1.68e-17 82.0
MS.gene060651.t1 AT1G67260 54.545 66 30 0 15 80 82 147 1.70e-17 82.0
MS.gene060651.t1 AT3G18550 55.738 61 27 0 19 79 147 207 1.52e-15 77.0
MS.gene060651.t1 AT3G18550 55.738 61 27 0 19 79 147 207 1.60e-15 77.0
MS.gene060651.t1 AT3G18550 55.738 61 27 0 19 79 147 207 1.64e-15 77.0
MS.gene060651.t1 AT3G18550 55.738 61 27 0 19 79 147 207 1.64e-15 77.0

Find 80 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCCAATCAAGTGCTTTAC+TGG 0.263366 2.2:+16494401 None:intergenic
TTACTAAACGACACCGTTTC+AGG 0.281541 2.2:+16494171 None:intergenic
TCGGGTGCCGGATTTGCTTC+TGG 0.295233 2.2:-16493808 MS.gene060651:CDS
TTCCGTGTGCCGGCACGAAT+TGG 0.322438 2.2:-16493772 MS.gene060651:CDS
CGACGTCAAGCGGGTGGTTC+GGG 0.323280 2.2:-16493826 MS.gene060651:CDS
AGTGGTGGCGGTGGCGGTGG+TGG 0.371377 2.2:-16494015 MS.gene060651:CDS
TTGACGGAGGTCACATTATA+AGG 0.376567 2.2:-16494554 MS.gene060651:CDS
TCGACGTCAAGCGGGTGGTT+CGG 0.377963 2.2:-16493827 MS.gene060651:CDS
ACTGGTAGTGGTGGCGGTGG+CGG 0.399543 2.2:-16494021 MS.gene060651:CDS
GGAATGGTGACGTGGAATTC+CGG 0.402921 2.2:-16494066 MS.gene060651:CDS
CACCATTCCATCAAAATTCC+CGG 0.405073 2.2:+16494080 None:intergenic
GGCGGTGGCGGTGGTGGTGA+TGG 0.409503 2.2:-16494009 MS.gene060651:CDS
GGTAGTGGTGGCGGTGGCGG+TGG 0.410519 2.2:-16494018 MS.gene060651:CDS
CAAGCGGGTGGTTCGGGTGC+CGG 0.418610 2.2:-16493820 MS.gene060651:CDS
AAAGCCACCAGAAGCAAATC+CGG 0.422822 2.2:+16493801 None:intergenic
CGTAAAATTCAATAGCGGTA+TGG 0.430606 2.2:+16494454 None:intergenic
ACGAACTGAAGGATTGTAAC+TGG 0.446835 2.2:+16493891 None:intergenic
TCGTGGTTCTTCGCCGTCGC+CGG 0.455216 2.2:-16493977 MS.gene060651:CDS
AAGATCGACTGGGCTTTGAT+AGG 0.456624 2.2:-16494425 MS.gene060651:CDS
GTAAAGTTTGCACGGCGAAA+GGG 0.459864 2.2:-16494506 MS.gene060651:CDS
GTAAAATTCAATAGCGGTAT+GGG 0.466376 2.2:+16494455 None:intergenic
AGTAAAGTTTGCACGGCGAA+AGG 0.477484 2.2:-16494507 MS.gene060651:CDS
AATGAACGGTGGAGAAATAG+TGG 0.479738 2.2:-16494580 MS.gene060651:CDS
TCAAAATGAAATACCTGAAA+CGG 0.483741 2.2:-16494184 MS.gene060651:CDS
TTGAAAAGAGTGGGTTTCCA+TGG 0.485913 2.2:+16494333 None:intergenic
ACAATCCTTCAGTTCGTGCT+TGG 0.487224 2.2:-16493885 MS.gene060651:CDS
AGACAGACGCGTCCGTCTCT+CGG 0.489881 2.2:-16494478 MS.gene060651:CDS
TTACGATGTTCAAGATCGAC+TGG 0.498681 2.2:-16494436 MS.gene060651:CDS
GGCCCAGTAAAGCACTTGAT+TGG 0.499781 2.2:-16494404 MS.gene060651:CDS
TGCATGGCGGTTTATCTGAC+AGG 0.502645 2.2:-16493732 MS.gene060651:CDS
GGTGCTGCTACTATTGATAC+TGG 0.504487 2.2:-16494039 MS.gene060651:CDS
CGTGTGCCGGCACGAATTGG+AGG 0.505599 2.2:-16493769 MS.gene060651:CDS
TGAGCGAGTTGGTCGATGGC+AGG 0.509170 2.2:+16494363 None:intergenic
GCTACTATTGATACTGGTAG+TGG 0.527342 2.2:-16494033 MS.gene060651:CDS
CTGTTGCTGTTTGAAAAGAG+TGG 0.528585 2.2:+16494323 None:intergenic
AACTCGCTCATCTTCCTCCA+TGG 0.528899 2.2:-16494350 MS.gene060651:CDS
CTCCAATTCGTGCCGGCACA+CGG 0.530036 2.2:+16493770 None:intergenic
TGGTTCTTCGCCGTCGCCGG+TGG 0.544579 2.2:-16493974 MS.gene060651:CDS
AGGATTGTAACTGGACTGAA+GGG 0.547857 2.2:+16493900 None:intergenic
AGACGGACGCGTCTGTCTCT+CGG 0.552176 2.2:+16494483 None:intergenic
TAGCGGTATGGGCCGAGAGA+CGG 0.558275 2.2:+16494466 None:intergenic
GGCTGATTATCGACGTCAAG+CGG 0.558536 2.2:-16493836 MS.gene060651:CDS
TGTTGCTGTTTGAAAAGAGT+GGG 0.562416 2.2:+16494324 None:intergenic
GTGACGTGGAATTCCGGTGG+TGG 0.563932 2.2:-16494060 MS.gene060651:CDS
GGTGCCGGATTTGCTTCTGG+TGG 0.566408 2.2:-16493805 MS.gene060651:CDS
AGCCAATCAAGTGCTTTACT+GGG 0.566827 2.2:+16494402 None:intergenic
AATTCAATGGCGAGAATCAG+AGG 0.571283 2.2:+16493696 None:intergenic
GGAGAAATAGTGGAAGTTGA+CGG 0.571965 2.2:-16494570 MS.gene060651:CDS
AAGGATTGTAACTGGACTGA+AGG 0.573483 2.2:+16493899 None:intergenic
TTCTGACCATACATCATCGC+CGG 0.576031 2.2:+16493946 None:intergenic
TGCGCCGGCGACATTTGTTG+CGG 0.586332 2.2:-16493857 MS.gene060651:CDS
GCTGATTATCGACGTCAAGC+GGG 0.587761 2.2:-16493835 MS.gene060651:CDS
TCGTGCTTGGATAGATGCGC+CGG 0.589204 2.2:-16493872 MS.gene060651:CDS
TCTATCCAAGCACGAACTGA+AGG 0.592530 2.2:+16493880 None:intergenic
GAATCAGTATCTTTCACAGA+GGG 0.594638 2.2:-16493926 MS.gene060651:CDS
AATAGTAGCAGCACCACCAC+CGG 0.596186 2.2:+16494047 None:intergenic
TCATCACCTCCAATTCGTGC+CGG 0.596792 2.2:+16493763 None:intergenic
GATTATGGAGGAATAATGAA+CGG 0.602608 2.2:-16494594 None:intergenic
TCAGCCGCAACAAATGTCGC+CGG 0.604069 2.2:+16493853 None:intergenic
TATGGAGGAATAATGAACGG+TGG 0.607439 2.2:-16494591 None:intergenic
GATACTGGTAGTGGTGGCGG+TGG 0.609847 2.2:-16494024 MS.gene060651:CDS
AAGATGAGCGAGTTGGTCGA+TGG 0.611325 2.2:+16494359 None:intergenic
ATTGATACTGGTAGTGGTGG+CGG 0.619170 2.2:-16494027 MS.gene060651:CDS
ACGAATTGGAGGTGATGATG+AGG 0.624959 2.2:-16493758 MS.gene060651:CDS
TACGATGTTCAAGATCGACT+GGG 0.627373 2.2:-16494435 MS.gene060651:CDS
ATGGAGGAAGATGAGCGAGT+TGG 0.629741 2.2:+16494352 None:intergenic
GATTATCGACGTCAAGCGGG+TGG 0.637375 2.2:-16493832 MS.gene060651:CDS
AAAAGAGTGGGTTTCCATGG+AGG 0.642106 2.2:+16494336 None:intergenic
TCGACATAGTAAAGTTTGCA+CGG 0.642697 2.2:-16494514 MS.gene060651:CDS
GGTCACATTATAAGGTCAAG+AGG 0.643297 2.2:-16494546 MS.gene060651:CDS
ATGGTGACGTGGAATTCCGG+TGG 0.669456 2.2:-16494063 MS.gene060651:CDS
AACATCGTAAAATTCAATAG+CGG 0.671747 2.2:+16494449 None:intergenic
GAATCAGAGGAAGCAGACGA+CGG 0.673313 2.2:+16493709 None:intergenic
ACTATTGATACTGGTAGTGG+TGG 0.683426 2.2:-16494030 MS.gene060651:CDS
GGTGATGATGAGGAGATGCA+TGG 0.698910 2.2:-16493748 MS.gene060651:CDS
GGATTGTAACTGGACTGAAG+GGG 0.704803 2.2:+16493901 None:intergenic
GAAATAGTGGAAGTTGACGG+AGG 0.730713 2.2:-16494567 MS.gene060651:CDS
AATCAGTATCTTTCACAGAG+GGG 0.746034 2.2:-16493925 MS.gene060651:CDS
AGAATCAGTATCTTTCACAG+AGG 0.756640 2.2:-16493927 MS.gene060651:CDS
GATGATGAGGAGATGCATGG+CGG 0.819316 2.2:-16493745 MS.gene060651:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AACATCGTAAAATTCAATAG+CGG + chr2.2:16493832-16493851 None:intergenic 25.0%
! TCAAAATGAAATACCTGAAA+CGG - chr2.2:16494094-16494113 MS.gene060651:CDS 25.0%
!!! TCAGGTATTTCATTTTGAAA+CGG + chr2.2:16494092-16494111 None:intergenic 25.0%
GTAAAATTCAATAGCGGTAT+GGG + chr2.2:16493826-16493845 None:intergenic 30.0%
AATCAGTATCTTTCACAGAG+GGG - chr2.2:16494353-16494372 MS.gene060651:CDS 35.0%
AGAATCAGTATCTTTCACAG+AGG - chr2.2:16494351-16494370 MS.gene060651:CDS 35.0%
CGTAAAATTCAATAGCGGTA+TGG + chr2.2:16493827-16493846 None:intergenic 35.0%
GAATCAGTATCTTTCACAGA+GGG - chr2.2:16494352-16494371 MS.gene060651:CDS 35.0%
TCGACATAGTAAAGTTTGCA+CGG - chr2.2:16493764-16493783 MS.gene060651:CDS 35.0%
!! TGTTGCTGTTTGAAAAGAGT+GGG + chr2.2:16493957-16493976 None:intergenic 35.0%
AAGGATTGTAACTGGACTGA+AGG + chr2.2:16494382-16494401 None:intergenic 40.0%
ACGAACTGAAGGATTGTAAC+TGG + chr2.2:16494390-16494409 None:intergenic 40.0%
AGCCAATCAAGTGCTTTACT+GGG + chr2.2:16493879-16493898 None:intergenic 40.0%
AGGATTGTAACTGGACTGAA+GGG + chr2.2:16494381-16494400 None:intergenic 40.0%
CACCATTCCATCAAAATTCC+CGG + chr2.2:16494201-16494220 None:intergenic 40.0%
GCTACTATTGATACTGGTAG+TGG - chr2.2:16494245-16494264 MS.gene060651:CDS 40.0%
GGTCACATTATAAGGTCAAG+AGG - chr2.2:16493732-16493751 MS.gene060651:CDS 40.0%
TACGATGTTCAAGATCGACT+GGG - chr2.2:16493843-16493862 MS.gene060651:CDS 40.0%
TTACGATGTTCAAGATCGAC+TGG - chr2.2:16493842-16493861 MS.gene060651:CDS 40.0%
TTACTAAACGACACCGTTTC+AGG + chr2.2:16494110-16494129 None:intergenic 40.0%
TTGACGGAGGTCACATTATA+AGG - chr2.2:16493724-16493743 MS.gene060651:CDS 40.0%
! GGAGAAATAGTGGAAGTTGA+CGG - chr2.2:16493708-16493727 MS.gene060651:CDS 40.0%
! TTGAAAAGAGTGGGTTTCCA+TGG + chr2.2:16493948-16493967 None:intergenic 40.0%
!! ACTATTGATACTGGTAGTGG+TGG - chr2.2:16494248-16494267 MS.gene060651:CDS 40.0%
!! ATTTTGATGGAATGGTGACG+TGG - chr2.2:16494204-16494223 MS.gene060651:CDS 40.0%
!! CTGTTGCTGTTTGAAAAGAG+TGG + chr2.2:16493958-16493977 None:intergenic 40.0%
AAAGCCACCAGAAGCAAATC+CGG + chr2.2:16494480-16494499 None:intergenic 45.0%
ACAATCCTTCAGTTCGTGCT+TGG - chr2.2:16494393-16494412 MS.gene060651:CDS 45.0%
AGTAAAGTTTGCACGGCGAA+AGG - chr2.2:16493771-16493790 MS.gene060651:CDS 45.0%
GAGCCAATCAAGTGCTTTAC+TGG + chr2.2:16493880-16493899 None:intergenic 45.0%
GGATTGTAACTGGACTGAAG+GGG + chr2.2:16494380-16494399 None:intergenic 45.0%
GTAAAGTTTGCACGGCGAAA+GGG - chr2.2:16493772-16493791 MS.gene060651:CDS 45.0%
TCTATCCAAGCACGAACTGA+AGG + chr2.2:16494401-16494420 None:intergenic 45.0%
TTCTGACCATACATCATCGC+CGG + chr2.2:16494335-16494354 None:intergenic 45.0%
! AAAAGAGTGGGTTTCCATGG+AGG + chr2.2:16493945-16493964 None:intergenic 45.0%
! AAGATCGACTGGGCTTTGAT+AGG - chr2.2:16493853-16493872 MS.gene060651:CDS 45.0%
! ACGAATTGGAGGTGATGATG+AGG - chr2.2:16494520-16494539 MS.gene060651:CDS 45.0%
! CTTTTCGCCGGGAATTTTGA+TGG - chr2.2:16494191-16494210 MS.gene060651:CDS 45.0%
! GAAATAGTGGAAGTTGACGG+AGG - chr2.2:16493711-16493730 MS.gene060651:CDS 45.0%
! GTTTTTCCGGCGATGATGTA+TGG - chr2.2:16494326-16494345 MS.gene060651:CDS 45.0%
! TTTGCTTCTGGTGGCTTTTC+TGG - chr2.2:16494482-16494501 MS.gene060651:CDS 45.0%
!! ATTGATACTGGTAGTGGTGG+CGG - chr2.2:16494251-16494270 MS.gene060651:CDS 45.0%
!! GGTGCTGCTACTATTGATAC+TGG - chr2.2:16494239-16494258 MS.gene060651:CDS 45.0%
!!! GGTGATGGTTTTGTGTTTCG+TGG - chr2.2:16494284-16494303 MS.gene060651:CDS 45.0%
AACTCGCTCATCTTCCTCCA+TGG - chr2.2:16493928-16493947 MS.gene060651:CDS 50.0%
AATAGTAGCAGCACCACCAC+CGG + chr2.2:16494234-16494253 None:intergenic 50.0%
ATCATCGCCGGAAAAACCAC+CGG + chr2.2:16494323-16494342 None:intergenic 50.0%
ATGGAGGAAGATGAGCGAGT+TGG + chr2.2:16493929-16493948 None:intergenic 50.0%
GAATCAGAGGAAGCAGACGA+CGG + chr2.2:16494572-16494591 None:intergenic 50.0%
GATGATGAGGAGATGCATGG+CGG - chr2.2:16494533-16494552 MS.gene060651:CDS 50.0%
GCTGATTATCGACGTCAAGC+GGG - chr2.2:16494443-16494462 MS.gene060651:CDS 50.0%
GGCTGATTATCGACGTCAAG+CGG - chr2.2:16494442-16494461 MS.gene060651:CDS 50.0%
TCATCACCTCCAATTCGTGC+CGG + chr2.2:16494518-16494537 None:intergenic 50.0%
TGCATGGCGGTTTATCTGAC+AGG - chr2.2:16494546-16494565 MS.gene060651:CDS 50.0%
! GGAATGGTGACGTGGAATTC+CGG - chr2.2:16494212-16494231 MS.gene060651:CDS 50.0%
! GGCCCAGTAAAGCACTTGAT+TGG - chr2.2:16493874-16493893 MS.gene060651:CDS 50.0%
! GGTGATGATGAGGAGATGCA+TGG - chr2.2:16494530-16494549 MS.gene060651:CDS 50.0%
!! AAGATGAGCGAGTTGGTCGA+TGG + chr2.2:16493922-16493941 None:intergenic 50.0%
!! CGCCGGGAATTTTGATGGAA+TGG - chr2.2:16494196-16494215 MS.gene060651:CDS 50.0%
!!! CTTTTCTGGTTTCCGTGTGC+CGG - chr2.2:16494496-16494515 MS.gene060651:CDS 50.0%
GATTATCGACGTCAAGCGGG+TGG - chr2.2:16494446-16494465 MS.gene060651:CDS 55.0%
TCAGCCGCAACAAATGTCGC+CGG + chr2.2:16494428-16494447 None:intergenic 55.0%
TCGTGCTTGGATAGATGCGC+CGG - chr2.2:16494406-16494425 MS.gene060651:CDS 55.0%
! AACAGCACGTGCTTTTCGCC+GGG - chr2.2:16494180-16494199 MS.gene060651:CDS 55.0%
! ATGGTGACGTGGAATTCCGG+TGG - chr2.2:16494215-16494234 MS.gene060651:CDS 55.0%
! CAACAGCACGTGCTTTTCGC+CGG - chr2.2:16494179-16494198 MS.gene060651:CDS 55.0%
AGACAGACGCGTCCGTCTCT+CGG - chr2.2:16493800-16493819 MS.gene060651:CDS 60.0%
AGACGGACGCGTCTGTCTCT+CGG + chr2.2:16493798-16493817 None:intergenic 60.0%
GTGACGTGGAATTCCGGTGG+TGG - chr2.2:16494218-16494237 MS.gene060651:CDS 60.0%
TAGCGGTATGGGCCGAGAGA+CGG + chr2.2:16493815-16493834 None:intergenic 60.0%
TCGACGTCAAGCGGGTGGTT+CGG - chr2.2:16494451-16494470 MS.gene060651:CDS 60.0%
! CTCCAATTCGTGCCGGCACA+CGG + chr2.2:16494511-16494530 None:intergenic 60.0%
! GGTGCCGGATTTGCTTCTGG+TGG - chr2.2:16494473-16494492 MS.gene060651:CDS 60.0%
! TGCGCCGGCGACATTTGTTG+CGG - chr2.2:16494421-16494440 MS.gene060651:CDS 60.0%
! TTCCGTGTGCCGGCACGAAT+TGG - chr2.2:16494506-16494525 MS.gene060651:CDS 60.0%
!! GATACTGGTAGTGGTGGCGG+TGG - chr2.2:16494254-16494273 MS.gene060651:CDS 60.0%
!! TCGGGTGCCGGATTTGCTTC+TGG - chr2.2:16494470-16494489 MS.gene060651:CDS 60.0%
!! TGAGCGAGTTGGTCGATGGC+AGG + chr2.2:16493918-16493937 None:intergenic 60.0%
CGACGTCAAGCGGGTGGTTC+GGG - chr2.2:16494452-16494471 MS.gene060651:CDS 65.0%
GCCGGAAAAACCACCGGCGA+CGG + chr2.2:16494317-16494336 None:intergenic 65.0%
TCGTGGTTCTTCGCCGTCGC+CGG - chr2.2:16494301-16494320 MS.gene060651:CDS 65.0%
! CGTGTGCCGGCACGAATTGG+AGG - chr2.2:16494509-16494528 MS.gene060651:CDS 65.0%
! GCCGTCGCCGGTGGTTTTTC+CGG - chr2.2:16494313-16494332 MS.gene060651:CDS 65.0%
!! ACTGGTAGTGGTGGCGGTGG+CGG - chr2.2:16494257-16494276 MS.gene060651:CDS 65.0%
TGGTTCTTCGCCGTCGCCGG+TGG - chr2.2:16494304-16494323 MS.gene060651:CDS 70.0%
! CAAGCGGGTGGTTCGGGTGC+CGG - chr2.2:16494458-16494477 MS.gene060651:CDS 70.0%
! GGCGGTGGCGGTGGTGGTGA+TGG - chr2.2:16494269-16494288 MS.gene060651:CDS 75.0%
!! AGTGGTGGCGGTGGCGGTGG+TGG - chr2.2:16494263-16494282 MS.gene060651:CDS 75.0%
!! GGTAGTGGTGGCGGTGGCGG+TGG - chr2.2:16494260-16494279 MS.gene060651:CDS 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 16493699 16494601 16493699 ID=MS.gene060651
chr2.2 mRNA 16493699 16494601 16493699 ID=MS.gene060651.t1;Parent=MS.gene060651
chr2.2 exon 16493699 16494601 16493699 ID=MS.gene060651.t1.exon1;Parent=MS.gene060651.t1
chr2.2 CDS 16493699 16494601 16493699 ID=cds.MS.gene060651.t1;Parent=MS.gene060651.t1
Gene Sequence

>MS.gene060651

ATGAACGGTGGAGAAATAGTGGAAGTTGACGGAGGTCACATTATAAGGTCAAGAGGTCGAAAAGATCGACATAGTAAAGTTTGCACGGCGAAAGGGCCGAGAGACAGACGCGTCCGTCTCTCGGCCCATACCGCTATTGAATTTTACGATGTTCAAGATCGACTGGGCTTTGATAGGCCCAGTAAAGCACTTGATTGGCTCATTAATAAAGCTAAGCCTGCCATCGACCAACTCGCTCATCTTCCTCCATGGAAACCCACTCTTTTCAAACAGCAACAGCAAAAGCAAAACGACGACGTTTTAGAGAAACAGAATCAAAACGAAAACGAAAACAACGAGTTTCGTTTTCTTCAGAATTTTAGCAATAATAGTAACAGCAACTGTTTCATTCCGTTTCAAAATGAAATACCTGAAACGGTGTCGTTTAGTAATGATTTGAAGCTTTCGCTTCAACAGAATCATAATCAGAATCAACACGTGCAACAGCACGTGCTTTTCGCCGGGAATTTTGATGGAATGGTGACGTGGAATTCCGGTGGTGGTGCTGCTACTATTGATACTGGTAGTGGTGGCGGTGGCGGTGGTGGTGATGGTTTTGTGTTTCGTGGTTCTTCGCCGTCGCCGGTGGTTTTTCCGGCGATGATGTATGGTCAGAATCAGTATCTTTCACAGAGGGGACCCCTTCAGTCCAGTTACAATCCTTCAGTTCGTGCTTGGATAGATGCGCCGGCGACATTTGTTGCGGCTGATTATCGACGTCAAGCGGGTGGTTCGGGTGCCGGATTTGCTTCTGGTGGCTTTTCTGGTTTCCGTGTGCCGGCACGAATTGGAGGTGATGATGAGGAGATGCATGGCGGTTTATCTGACAGGCCGTCGTCTGCTTCCTCTGATTCTCGCCATTGA
Protein sequence

>MS.gene060651.t1

MNGGEIVEVDGGHIIRSRGRKDRHSKVCTAKGPRDRRVRLSAHTAIEFYDVQDRLGFDRPSKALDWLINKAKPAIDQLAHLPPWKPTLFKQQQQKQNDDVLEKQNQNENENNEFRFLQNFSNNSNSNCFIPFQNEIPETVSFSNDLKLSLQQNHNQNQHVQQHVLFAGNFDGMVTWNSGGGAATIDTGSGGGGGGGDGFVFRGSSPSPVVFPAMMYGQNQYLSQRGPLQSSYNPSVRAWIDAPATFVAADYRRQAGGSGAGFASGGFSGFRVPARIGGDDEEMHGGLSDRPSSASSDSRH