Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060698.t1 | XP_013464662.1 | 95.6 | 68 | 3 | 0 | 5 | 72 | 104 | 171 | 2.70E-28 | 134 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060698.t1 | Q9S7A9 | 66.0 | 47 | 15 | 1 | 5 | 51 | 100 | 145 | 1.0e-07 | 57.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060698.t1 | A0A072VA58 | 95.6 | 68 | 3 | 0 | 5 | 72 | 104 | 171 | 1.9e-28 | 134.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene060698.t1 | TF | SBP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060698.t1 | MTR_2g078770 | 95.588 | 68 | 3 | 0 | 5 | 72 | 104 | 171 | 6.09e-43 | 136 |
| MS.gene060698.t1 | MTR_8g463140 | 55.714 | 70 | 29 | 1 | 5 | 72 | 111 | 180 | 1.35e-17 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060698.t1 | AT1G53160 | 65.957 | 47 | 15 | 1 | 5 | 51 | 100 | 145 | 1.19e-12 | 59.7 |
| MS.gene060698.t1 | AT1G53160 | 65.957 | 47 | 15 | 1 | 5 | 51 | 100 | 145 | 1.22e-12 | 59.7 |
| MS.gene060698.t1 | AT1G53160 | 65.957 | 47 | 15 | 1 | 5 | 51 | 100 | 145 | 1.22e-12 | 59.7 |
| MS.gene060698.t1 | AT3G15270 | 74.359 | 39 | 10 | 0 | 5 | 43 | 109 | 147 | 2.79e-12 | 58.5 |
| MS.gene060698.t1 | AT2G33810 | 86.667 | 30 | 4 | 0 | 5 | 34 | 100 | 129 | 5.78e-11 | 54.3 |
Find 9 sgRNAs with CRISPR-Local
Find 9 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGAACATAACAAGTAGTTC+TGG | 0.412406 | 2.1:+21709439 | MS.gene060698:CDS |
| AGCAAAATCATGATGGTGAT+TGG | 0.426111 | 2.1:+21709404 | MS.gene060698:CDS |
| TGCACTGAAGGAAGCGGTAC+TGG | 0.443322 | 2.1:+21709379 | MS.gene060698:CDS |
| CGGCGTCGTAAAAGCACTGC+TGG | 0.466987 | 2.1:+21709352 | MS.gene060698:CDS |
| ACTGCTGGAACTTGCACTGA+AGG | 0.529046 | 2.1:+21709367 | MS.gene060698:CDS |
| AACCTTTGATGAGTGTGCGC+AGG | 0.591425 | 2.1:+21709260 | None:intergenic |
| GGAACTTGCACTGAAGGAAG+CGG | 0.610870 | 2.1:+21709373 | MS.gene060698:CDS |
| ACTGGCCAGCAAAATCATGA+TGG | 0.639807 | 2.1:+21709397 | MS.gene060698:CDS |
| GCTTAGCGCGACACAATGAA+CGG | 0.657384 | 2.1:+21709332 | MS.gene060698:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGAACATAACAAGTAGTTC+TGG | + | chr2.1:21709439-21709458 | MS.gene060698:CDS | 30.0% | |
| ! | AATCACCATCATGATTTTGC+TGG | - | chr2.1:21709405-21709424 | None:intergenic | 35.0% |
| ! | AGCAAAATCATGATGGTGAT+TGG | + | chr2.1:21709404-21709423 | MS.gene060698:CDS | 35.0% |
| ACTGGCCAGCAAAATCATGA+TGG | + | chr2.1:21709397-21709416 | MS.gene060698:CDS | 45.0% | |
| ACTGCTGGAACTTGCACTGA+AGG | + | chr2.1:21709367-21709386 | MS.gene060698:CDS | 50.0% | |
| GCTTAGCGCGACACAATGAA+CGG | + | chr2.1:21709332-21709351 | MS.gene060698:CDS | 50.0% | |
| GGAACTTGCACTGAAGGAAG+CGG | + | chr2.1:21709373-21709392 | MS.gene060698:CDS | 50.0% | |
| TGCACTGAAGGAAGCGGTAC+TGG | + | chr2.1:21709379-21709398 | MS.gene060698:CDS | 55.0% | |
| !! | CGGCGTCGTAAAAGCACTGC+TGG | + | chr2.1:21709352-21709371 | MS.gene060698:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 21709268 | 21709486 | 21709268 | ID=MS.gene060698 |
| chr2.1 | mRNA | 21709268 | 21709486 | 21709268 | ID=MS.gene060698.t1;Parent=MS.gene060698 |
| chr2.1 | exon | 21709268 | 21709486 | 21709268 | ID=MS.gene060698.t1.exon1;Parent=MS.gene060698.t1 |
| chr2.1 | CDS | 21709268 | 21709486 | 21709268 | ID=cds.MS.gene060698.t1;Parent=MS.gene060698.t1 |
| Gene Sequence |
| Protein sequence |
