Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060729.t1 | XP_003605525.1 | 69.8 | 232 | 69 | 1 | 1 | 232 | 1 | 231 | 4.00E-81 | 311.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060729.t1 | Q9FJK3 | 53.8 | 160 | 73 | 1 | 1 | 160 | 1 | 159 | 9.1e-42 | 171.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060729.t1 | G7JN85 | 69.8 | 232 | 69 | 1 | 1 | 232 | 1 | 231 | 2.9e-81 | 311.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene060729.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060729.t1 | MTR_4g032620 | 76.724 | 232 | 53 | 1 | 1 | 232 | 1 | 231 | 2.56e-130 | 367 |
| MS.gene060729.t1 | MTR_8g036130 | 58.065 | 248 | 87 | 4 | 1 | 232 | 1 | 247 | 2.66e-87 | 258 |
| MS.gene060729.t1 | MTR_4g031910 | 55.603 | 232 | 100 | 2 | 1 | 232 | 1 | 229 | 4.58e-78 | 234 |
| MS.gene060729.t1 | MTR_4g032290 | 53.061 | 245 | 88 | 4 | 1 | 232 | 1 | 231 | 1.11e-71 | 218 |
| MS.gene060729.t1 | MTR_4g032260 | 64.103 | 156 | 56 | 0 | 1 | 156 | 1 | 156 | 7.93e-60 | 186 |
| MS.gene060729.t1 | MTR_3g065100 | 48.707 | 232 | 110 | 4 | 1 | 224 | 1 | 231 | 8.52e-58 | 183 |
| MS.gene060729.t1 | MTR_3g466980 | 42.381 | 210 | 109 | 2 | 1 | 199 | 1 | 209 | 9.89e-53 | 170 |
| MS.gene060729.t1 | MTR_2g035610 | 66.116 | 121 | 41 | 0 | 1 | 121 | 1 | 121 | 2.67e-46 | 150 |
| MS.gene060729.t1 | MTR_3g466830 | 44.785 | 163 | 85 | 2 | 3 | 163 | 2 | 161 | 5.98e-45 | 148 |
| MS.gene060729.t1 | MTR_5g047580 | 41.667 | 156 | 89 | 2 | 1 | 156 | 1 | 154 | 3.37e-41 | 138 |
| MS.gene060729.t1 | MTR_1g090697 | 40.506 | 158 | 92 | 2 | 1 | 158 | 1 | 156 | 1.14e-38 | 132 |
| MS.gene060729.t1 | MTR_4g063790 | 42.581 | 155 | 89 | 0 | 1 | 155 | 1 | 155 | 1.21e-38 | 132 |
| MS.gene060729.t1 | MTR_3g466890 | 42.308 | 156 | 89 | 1 | 3 | 158 | 2 | 156 | 1.36e-37 | 129 |
| MS.gene060729.t1 | MTR_1g077390 | 39.103 | 156 | 93 | 2 | 1 | 156 | 1 | 154 | 1.34e-36 | 126 |
| MS.gene060729.t1 | MTR_1g090710 | 37.975 | 158 | 97 | 1 | 1 | 158 | 1 | 157 | 1.62e-35 | 124 |
| MS.gene060729.t1 | MTR_1g084950 | 42.308 | 156 | 88 | 2 | 1 | 156 | 1 | 154 | 3.24e-35 | 123 |
| MS.gene060729.t1 | MTR_3g467080 | 37.821 | 156 | 97 | 0 | 1 | 156 | 1 | 156 | 6.63e-35 | 122 |
| MS.gene060729.t1 | MTR_7g011950 | 41.026 | 156 | 90 | 2 | 1 | 156 | 1 | 154 | 1.80e-34 | 121 |
| MS.gene060729.t1 | MTR_3g031100 | 38.182 | 165 | 102 | 0 | 1 | 165 | 1 | 165 | 2.63e-34 | 123 |
| MS.gene060729.t1 | MTR_4g028800 | 79.167 | 72 | 13 | 1 | 1 | 72 | 1 | 70 | 8.71e-34 | 116 |
| MS.gene060729.t1 | MTR_2g016210 | 37.195 | 164 | 103 | 0 | 1 | 164 | 1 | 164 | 1.75e-33 | 121 |
| MS.gene060729.t1 | MTR_4g028720 | 41.667 | 156 | 89 | 2 | 1 | 156 | 1 | 154 | 3.15e-33 | 118 |
| MS.gene060729.t1 | MTR_1g090783 | 38.961 | 154 | 92 | 2 | 3 | 156 | 2 | 153 | 3.59e-33 | 117 |
| MS.gene060729.t1 | MTR_5g075380 | 38.961 | 154 | 93 | 1 | 1 | 154 | 1 | 153 | 5.62e-33 | 117 |
| MS.gene060729.t1 | MTR_3g031240 | 32.716 | 162 | 109 | 0 | 1 | 162 | 1 | 162 | 7.69e-30 | 110 |
| MS.gene060729.t1 | MTR_1g077320 | 37.179 | 156 | 96 | 2 | 1 | 156 | 1 | 154 | 5.00e-29 | 107 |
| MS.gene060729.t1 | MTR_3g465410 | 41.270 | 126 | 74 | 0 | 29 | 154 | 1 | 126 | 2.65e-27 | 102 |
| MS.gene060729.t1 | MTR_7g055800 | 47.899 | 119 | 34 | 2 | 1 | 119 | 1 | 91 | 6.28e-27 | 99.8 |
| MS.gene060729.t1 | MTR_1g077300 | 38.365 | 159 | 88 | 3 | 1 | 156 | 1 | 152 | 2.85e-26 | 100 |
| MS.gene060729.t1 | MTR_3g466900 | 37.008 | 127 | 79 | 1 | 32 | 158 | 2 | 127 | 2.48e-25 | 96.7 |
| MS.gene060729.t1 | MTR_3g466930 | 39.062 | 128 | 75 | 3 | 32 | 158 | 2 | 127 | 2.98e-25 | 96.7 |
| MS.gene060729.t1 | MTR_5g047560 | 40.678 | 118 | 53 | 2 | 1 | 118 | 1 | 101 | 3.44e-25 | 96.7 |
| MS.gene060729.t1 | MTR_2g035580 | 35.256 | 156 | 100 | 1 | 1 | 156 | 1 | 155 | 5.87e-24 | 94.0 |
| MS.gene060729.t1 | MTR_4g019670 | 32.278 | 158 | 103 | 2 | 3 | 157 | 6 | 162 | 1.29e-21 | 93.2 |
| MS.gene060729.t1 | MTR_7g106510 | 31.250 | 160 | 106 | 2 | 1 | 157 | 4 | 162 | 1.79e-21 | 92.8 |
| MS.gene060729.t1 | MTR_4g032270 | 49.000 | 100 | 45 | 4 | 135 | 232 | 36 | 131 | 2.73e-21 | 86.3 |
| MS.gene060729.t1 | MTR_7g055940 | 46.667 | 105 | 28 | 2 | 1 | 105 | 1 | 77 | 7.29e-21 | 83.6 |
| MS.gene060729.t1 | MTR_7g055790 | 46.364 | 110 | 41 | 1 | 1 | 110 | 59 | 150 | 9.12e-20 | 82.8 |
| MS.gene060729.t1 | MTR_3g093900 | 30.636 | 173 | 112 | 4 | 1 | 171 | 4 | 170 | 8.10e-17 | 79.3 |
| MS.gene060729.t1 | MTR_6g018920 | 30.000 | 160 | 97 | 6 | 1 | 154 | 1 | 151 | 3.09e-14 | 71.2 |
| MS.gene060729.t1 | MTR_1g114730 | 34.188 | 117 | 62 | 4 | 1 | 103 | 1 | 116 | 5.12e-13 | 67.8 |
| MS.gene060729.t1 | MTR_2g035590 | 47.126 | 87 | 46 | 0 | 74 | 160 | 1 | 87 | 8.36e-13 | 62.8 |
| MS.gene060729.t1 | MTR_6g005450 | 36.441 | 118 | 60 | 5 | 1 | 104 | 1 | 117 | 2.11e-12 | 65.9 |
| MS.gene060729.t1 | MTR_6g005440 | 37.288 | 118 | 59 | 5 | 1 | 104 | 1 | 117 | 4.15e-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060729.t1 | AT5G48670 | 53.750 | 160 | 73 | 1 | 1 | 160 | 1 | 159 | 3.05e-49 | 164 |
| MS.gene060729.t1 | AT5G26630 | 39.267 | 191 | 114 | 2 | 1 | 190 | 1 | 190 | 1.69e-35 | 125 |
| MS.gene060729.t1 | AT5G26650 | 41.176 | 153 | 89 | 1 | 4 | 156 | 2 | 153 | 9.89e-35 | 127 |
| MS.gene060729.t1 | AT1G65300 | 41.830 | 153 | 88 | 1 | 3 | 155 | 2 | 153 | 1.54e-34 | 124 |
| MS.gene060729.t1 | AT5G27960 | 40.523 | 153 | 90 | 1 | 4 | 156 | 2 | 153 | 3.10e-34 | 125 |
| MS.gene060729.t1 | AT3G05860 | 39.333 | 150 | 90 | 1 | 1 | 150 | 1 | 149 | 4.27e-33 | 119 |
| MS.gene060729.t1 | AT5G27810 | 47.788 | 113 | 59 | 0 | 29 | 141 | 1 | 113 | 5.45e-33 | 116 |
| MS.gene060729.t1 | AT3G05860 | 39.333 | 150 | 90 | 1 | 1 | 150 | 1 | 149 | 1.69e-32 | 119 |
| MS.gene060729.t1 | AT3G05860 | 39.333 | 150 | 90 | 1 | 1 | 150 | 1 | 149 | 2.20e-32 | 119 |
| MS.gene060729.t1 | AT1G65330 | 38.636 | 176 | 95 | 3 | 3 | 173 | 2 | 169 | 5.42e-32 | 118 |
| MS.gene060729.t1 | AT1G31630 | 32.759 | 174 | 113 | 3 | 3 | 176 | 2 | 171 | 1.79e-29 | 112 |
| MS.gene060729.t1 | AT1G31640 | 34.437 | 151 | 97 | 2 | 3 | 153 | 2 | 150 | 6.86e-28 | 110 |
| MS.gene060729.t1 | AT2G28700 | 35.526 | 152 | 93 | 2 | 1 | 150 | 1 | 149 | 1.44e-26 | 105 |
| MS.gene060729.t1 | AT5G26580 | 35.484 | 155 | 85 | 2 | 1 | 155 | 1 | 140 | 1.72e-24 | 99.8 |
| MS.gene060729.t1 | AT1G22590 | 30.128 | 156 | 105 | 1 | 1 | 156 | 1 | 152 | 1.72e-23 | 93.2 |
| MS.gene060729.t1 | AT5G06500 | 35.065 | 154 | 98 | 1 | 1 | 154 | 1 | 152 | 3.34e-21 | 89.0 |
| MS.gene060729.t1 | AT2G40210 | 41.667 | 120 | 70 | 0 | 1 | 120 | 1 | 120 | 1.60e-20 | 89.4 |
| MS.gene060729.t1 | AT5G55690 | 43.038 | 79 | 38 | 2 | 1 | 72 | 1 | 79 | 8.86e-13 | 66.6 |
| MS.gene060729.t1 | AT5G55690 | 43.038 | 79 | 38 | 2 | 1 | 72 | 1 | 79 | 8.86e-13 | 66.6 |
| MS.gene060729.t1 | AT5G58890 | 37.374 | 99 | 54 | 3 | 1 | 92 | 1 | 98 | 2.82e-12 | 65.1 |
Find 0 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATTCAGAACAATGAATG+TGG | + | 33290:8198-8217 | MS.gene060729:CDS | 25.0% |
| ! | AAATTCAGAACAATGAATGT+GGG | + | 33290:8199-8218 | MS.gene060729:CDS | 25.0% |
| ! | AAGAGAAACTAAAGATACAA+AGG | + | 33290:8284-8303 | MS.gene060729:CDS | 25.0% |
| ! | ATTGAAAGTCAATCTTTCAT+GGG | + | 33290:8586-8605 | MS.gene060729:CDS | 25.0% |
| ! | TACATTCAGTAATGAAGAAA+TGG | + | 33290:8510-8529 | MS.gene060729:CDS | 25.0% |
| ! | TATGCAGAATAATATGTCAT+TGG | + | 33290:8369-8388 | MS.gene060729:CDS | 25.0% |
| ! | TCTTCATTACTGAATGTAAA+TGG | - | 33290:8508-8527 | MS.gene060729:intergenic | 25.0% |
| ! | TTGATTCAAAACATCTTTGT+TGG | + | 33290:8392-8411 | MS.gene060729:CDS | 25.0% |
| AAGAGACTTACATGAAAGAA+AGG | + | 33290:8248-8267 | MS.gene060729:CDS | 30.0% | |
| AATGAAGAAATGGCAATGAT+GGG | + | 33290:8520-8539 | MS.gene060729:CDS | 30.0% | |
| AATTCAGAACAATGAATGTG+GGG | + | 33290:8200-8219 | MS.gene060729:CDS | 30.0% | |
| AGAGACTTACATGAAAGAAA+GGG | + | 33290:8249-8268 | MS.gene060729:CDS | 30.0% | |
| CATTGAAAGTCAATCTTTCA+TGG | + | 33290:8585-8604 | MS.gene060729:CDS | 30.0% | |
| TAATGAAGAAATGGCAATGA+TGG | + | 33290:8519-8538 | MS.gene060729:CDS | 30.0% | |
| TCATTACTGAATGTAAATGG+TGG | - | 33290:8505-8524 | MS.gene060729:intergenic | 30.0% | |
| ! | AGTAGATTTGGTAAAACTCT+AGG | - | 33290:8664-8683 | MS.gene060729:intergenic | 30.0% |
| AAGCAACATACAATAGAAGG+CGG | + | 33290:8044-8063 | MS.gene060729:CDS | 35.0% | |
| AGAATCATCATCGACAATGA+AGG | - | 33290:8020-8039 | MS.gene060729:intergenic | 35.0% | |
| ATGTTGTCGCCATTATTCAT+AGG | - | 33290:8568-8587 | MS.gene060729:intergenic | 35.0% | |
| ATTCAGAACAATGAATGTGG+GGG | + | 33290:8201-8220 | MS.gene060729:CDS | 35.0% | |
| GGAAAGCAACATACAATAGA+AGG | + | 33290:8041-8060 | MS.gene060729:CDS | 35.0% | |
| GTTGATTGACCACAAATTGA+AGG | + | 33290:8414-8433 | MS.gene060729:CDS | 35.0% | |
| TCATTGTCGATGATGATTCT+AGG | + | 33290:8020-8039 | MS.gene060729:CDS | 35.0% | |
| TTACTGAATGTAAATGGTGG+TGG | - | 33290:8502-8521 | MS.gene060729:intergenic | 35.0% | |
| ! | ATAGCTTGTGCCATAGTTTA+TGG | + | 33290:8115-8134 | MS.gene060729:CDS | 35.0% |
| ! | CATGAAAGAAAGGGTTCTAA+AGG | + | 33290:8258-8277 | MS.gene060729:CDS | 35.0% |
| ! | GAATAGTAATGGAGATGAGT+CGG | + | 33290:8618-8637 | MS.gene060729:CDS | 35.0% |
| ! | GATACAAAGGCATGATAACA+AGG | + | 33290:8297-8316 | MS.gene060729:CDS | 35.0% |
| !! | ATGTAAATGGTGGTGGTATT+TGG | - | 33290:8495-8514 | MS.gene060729:intergenic | 35.0% |
| !! | GGCTTGATGATGAATAGTAA+TGG | + | 33290:8607-8626 | MS.gene060729:CDS | 35.0% |
| !!! | TGAATTTTGAGAGCACTGTT+TGG | - | 33290:8186-8205 | MS.gene060729:intergenic | 35.0% |
| AACATACAATAGAAGGCGGA+AGG | + | 33290:8048-8067 | MS.gene060729:CDS | 40.0% | |
| AAGGCATGATAACAAGGAGA+TGG | + | 33290:8303-8322 | MS.gene060729:CDS | 40.0% | |
| ACATACAATAGAAGGCGGAA+GGG | + | 33290:8049-8068 | MS.gene060729:CDS | 40.0% | |
| GATTGACCACAAATTGAAGG+AGG | + | 33290:8417-8436 | MS.gene060729:CDS | 40.0% | |
| TACCAACCTCCTTCAATTTG+TGG | - | 33290:8426-8445 | MS.gene060729:intergenic | 40.0% | |
| TATGAACTAAGCACTCTGTG+TGG | + | 33290:8088-8107 | MS.gene060729:CDS | 40.0% | |
| TTGTCGCCATTATTCATAGG+CGG | - | 33290:8565-8584 | MS.gene060729:intergenic | 40.0% | |
| !! | AATTGAAGGAGGTTGGTAGA+AGG | + | 33290:8428-8447 | MS.gene060729:CDS | 40.0% |
| CTCGTAGGGTCCATAAACTA+TGG | - | 33290:8128-8147 | MS.gene060729:intergenic | 45.0% | |
| GACCACAAATTGAAGGAGGT+TGG | + | 33290:8421-8440 | MS.gene060729:CDS | 45.0% | |
| GGAATGCCGCCTATGAATAA+TGG | + | 33290:8556-8575 | MS.gene060729:CDS | 45.0% | |
| ATCTCTGGTTGAGGCTCGTA+GGG | - | 33290:8142-8161 | MS.gene060729:intergenic | 50.0% | |
| GGGGGAGAAGAGCAACAAAA+CGG | + | 33290:8219-8238 | MS.gene060729:CDS | 50.0% | |
| TGGTAGAAGGTTGGAAGCAG+AGG | + | 33290:8441-8460 | MS.gene060729:CDS | 50.0% | |
| ! | CTAGGATCGACTTCGCCAAA+TGG | - | 33290:8646-8665 | MS.gene060729:intergenic | 50.0% |
| !! | GAAGGAGGTTGGTAGAAGGT+TGG | + | 33290:8432-8451 | MS.gene060729:CDS | 50.0% |
| ACGAGCCTCAACCAGAGATC+TGG | + | 33290:8143-8162 | MS.gene060729:CDS | 55.0% | |
| AGCACTGTTTGGACTCCCTG+TGG | - | 33290:8175-8194 | MS.gene060729:intergenic | 55.0% | |
| GATCTCTGGTTGAGGCTCGT+AGG | - | 33290:8143-8162 | MS.gene060729:intergenic | 55.0% | |
| GCGGAAGGGTCTACTGAAGA+AGG | + | 33290:8063-8082 | MS.gene060729:CDS | 55.0% | |
| GTTTGGACTCCCTGTGGTGA+CGG | - | 33290:8169-8188 | MS.gene060729:intergenic | 55.0% | |
| TCATAGGCGGCATTCCAGCA+TGG | - | 33290:8552-8571 | MS.gene060729:intergenic | 55.0% | |
| !! | ATGATGGGTCCTAGCCATGC+TGG | + | 33290:8535-8554 | MS.gene060729:CDS | 55.0% |
| !! | GGAGATGAGTCGGTGCCATT+TGG | + | 33290:8628-8647 | MS.gene060729:CDS | 55.0% |
| AGAGATCTGGCCGTCACCAC+AGG | + | 33290:8156-8175 | MS.gene060729:CDS | 60.0% | |
| GACGGCCAGATCTCTGGTTG+AGG | - | 33290:8151-8170 | MS.gene060729:intergenic | 60.0% | |
| GAGATCTGGCCGTCACCACA+GGG | + | 33290:8157-8176 | MS.gene060729:CDS | 60.0% | |
| !! | TGTGGTGACGGCCAGATCTC+TGG | - | 33290:8157-8176 | MS.gene060729:intergenic | 60.0% |
| GGCGGCATTCCAGCATGGCT+AGG | - | 33290:8547-8566 | MS.gene060729:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 33290 | gene | 7992 | 8690 | 7992 | ID=MS.gene060729 |
| 33290 | mRNA | 7992 | 8690 | 7992 | ID=MS.gene060729.t1;Parent=MS.gene060729 |
| 33290 | exon | 7992 | 8690 | 7992 | ID=MS.gene060729.t1.exon1;Parent=MS.gene060729.t1 |
| 33290 | CDS | 7992 | 8690 | 7992 | ID=cds.MS.gene060729.t1;Parent=MS.gene060729.t1 |
| Gene Sequence |
| Protein sequence |
