AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060730.t1 MTR_4g032620 76.724 232 53 1 1 232 1 231 2.56e-130 367
MS.gene060730.t1 MTR_8g036130 58.065 248 87 4 1 232 1 247 2.66e-87 258
MS.gene060730.t1 MTR_4g031910 55.603 232 100 2 1 232 1 229 4.58e-78 234
MS.gene060730.t1 MTR_4g032290 53.061 245 88 4 1 232 1 231 1.11e-71 218
MS.gene060730.t1 MTR_4g032260 64.103 156 56 0 1 156 1 156 7.93e-60 186
MS.gene060730.t1 MTR_3g065100 48.707 232 110 4 1 224 1 231 8.52e-58 183
MS.gene060730.t1 MTR_3g466980 42.381 210 109 2 1 199 1 209 9.89e-53 170
MS.gene060730.t1 MTR_2g035610 66.116 121 41 0 1 121 1 121 2.67e-46 150
MS.gene060730.t1 MTR_3g466830 44.785 163 85 2 3 163 2 161 5.98e-45 148
MS.gene060730.t1 MTR_5g047580 41.667 156 89 2 1 156 1 154 3.37e-41 138
MS.gene060730.t1 MTR_1g090697 40.506 158 92 2 1 158 1 156 1.14e-38 132
MS.gene060730.t1 MTR_4g063790 42.581 155 89 0 1 155 1 155 1.21e-38 132
MS.gene060730.t1 MTR_3g466890 42.308 156 89 1 3 158 2 156 1.36e-37 129
MS.gene060730.t1 MTR_1g077390 39.103 156 93 2 1 156 1 154 1.34e-36 126
MS.gene060730.t1 MTR_1g090710 37.975 158 97 1 1 158 1 157 1.62e-35 124
MS.gene060730.t1 MTR_1g084950 42.308 156 88 2 1 156 1 154 3.24e-35 123
MS.gene060730.t1 MTR_3g467080 37.821 156 97 0 1 156 1 156 6.63e-35 122
MS.gene060730.t1 MTR_7g011950 41.026 156 90 2 1 156 1 154 1.80e-34 121
MS.gene060730.t1 MTR_3g031100 38.182 165 102 0 1 165 1 165 2.63e-34 123
MS.gene060730.t1 MTR_4g028800 79.167 72 13 1 1 72 1 70 8.71e-34 116
MS.gene060730.t1 MTR_2g016210 37.195 164 103 0 1 164 1 164 1.75e-33 121
MS.gene060730.t1 MTR_4g028720 41.667 156 89 2 1 156 1 154 3.15e-33 118
MS.gene060730.t1 MTR_1g090783 38.961 154 92 2 3 156 2 153 3.59e-33 117
MS.gene060730.t1 MTR_5g075380 38.961 154 93 1 1 154 1 153 5.62e-33 117
MS.gene060730.t1 MTR_3g031240 32.716 162 109 0 1 162 1 162 7.69e-30 110
MS.gene060730.t1 MTR_1g077320 37.179 156 96 2 1 156 1 154 5.00e-29 107
MS.gene060730.t1 MTR_3g465410 41.270 126 74 0 29 154 1 126 2.65e-27 102
MS.gene060730.t1 MTR_7g055800 47.899 119 34 2 1 119 1 91 6.28e-27 99.8
MS.gene060730.t1 MTR_1g077300 38.365 159 88 3 1 156 1 152 2.85e-26 100
MS.gene060730.t1 MTR_3g466900 37.008 127 79 1 32 158 2 127 2.48e-25 96.7
MS.gene060730.t1 MTR_3g466930 39.062 128 75 3 32 158 2 127 2.98e-25 96.7
MS.gene060730.t1 MTR_5g047560 40.678 118 53 2 1 118 1 101 3.44e-25 96.7
MS.gene060730.t1 MTR_2g035580 35.256 156 100 1 1 156 1 155 5.87e-24 94.0
MS.gene060730.t1 MTR_4g019670 32.278 158 103 2 3 157 6 162 1.29e-21 93.2
MS.gene060730.t1 MTR_7g106510 31.250 160 106 2 1 157 4 162 1.79e-21 92.8
MS.gene060730.t1 MTR_4g032270 49.000 100 45 4 135 232 36 131 2.73e-21 86.3
MS.gene060730.t1 MTR_7g055940 46.667 105 28 2 1 105 1 77 7.29e-21 83.6
MS.gene060730.t1 MTR_7g055790 46.364 110 41 1 1 110 59 150 9.12e-20 82.8
MS.gene060730.t1 MTR_3g093900 30.636 173 112 4 1 171 4 170 8.10e-17 79.3
MS.gene060730.t1 MTR_6g018920 30.000 160 97 6 1 154 1 151 3.09e-14 71.2
MS.gene060730.t1 MTR_1g114730 34.188 117 62 4 1 103 1 116 5.12e-13 67.8
MS.gene060730.t1 MTR_2g035590 47.126 87 46 0 74 160 1 87 8.36e-13 62.8
MS.gene060730.t1 MTR_6g005450 36.441 118 60 5 1 104 1 117 2.11e-12 65.9
MS.gene060730.t1 MTR_6g005440 37.288 118 59 5 1 104 1 117 4.15e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060730.t1 AT5G48670 53.750 160 73 1 1 160 1 159 3.05e-49 164
MS.gene060730.t1 AT5G26630 39.267 191 114 2 1 190 1 190 1.69e-35 125
MS.gene060730.t1 AT5G26650 41.176 153 89 1 4 156 2 153 9.89e-35 127
MS.gene060730.t1 AT1G65300 41.830 153 88 1 3 155 2 153 1.54e-34 124
MS.gene060730.t1 AT5G27960 40.523 153 90 1 4 156 2 153 3.10e-34 125
MS.gene060730.t1 AT3G05860 39.333 150 90 1 1 150 1 149 4.27e-33 119
MS.gene060730.t1 AT5G27810 47.788 113 59 0 29 141 1 113 5.45e-33 116
MS.gene060730.t1 AT3G05860 39.333 150 90 1 1 150 1 149 1.69e-32 119
MS.gene060730.t1 AT3G05860 39.333 150 90 1 1 150 1 149 2.20e-32 119
MS.gene060730.t1 AT1G65330 38.636 176 95 3 3 173 2 169 5.42e-32 118
MS.gene060730.t1 AT1G31630 32.759 174 113 3 3 176 2 171 1.79e-29 112
MS.gene060730.t1 AT1G31640 34.437 151 97 2 3 153 2 150 6.86e-28 110
MS.gene060730.t1 AT2G28700 35.526 152 93 2 1 150 1 149 1.44e-26 105
MS.gene060730.t1 AT5G26580 35.484 155 85 2 1 155 1 140 1.72e-24 99.8
MS.gene060730.t1 AT1G22590 30.128 156 105 1 1 156 1 152 1.72e-23 93.2
MS.gene060730.t1 AT5G06500 35.065 154 98 1 1 154 1 152 3.34e-21 89.0
MS.gene060730.t1 AT2G40210 41.667 120 70 0 1 120 1 120 1.60e-20 89.4
MS.gene060730.t1 AT5G55690 43.038 79 38 2 1 72 1 79 8.86e-13 66.6
MS.gene060730.t1 AT5G55690 43.038 79 38 2 1 72 1 79 8.86e-13 66.6
MS.gene060730.t1 AT5G58890 37.374 99 54 3 1 92 1 98 2.82e-12 65.1

Find 60 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGCTTGTGCCATAGTTTA+TGG 0.132446 4.3:-68498872 MS.gene060730:CDS
CACAACAATGACTAGGAAAA+AGG 0.228363 4.3:-68499002 None:intergenic
TCTTCATTACTGAATGTAAA+TGG 0.245246 4.3:+68498482 None:intergenic
TCATTGTCGATGATGATTCT+AGG 0.260008 4.3:-68498967 MS.gene060730:CDS
TTCATTTAAGGTAGTAGATT+TGG 0.271423 4.3:+68498314 None:intergenic
ATGTAAATGGTGGTGGTATT+TGG 0.308969 4.3:+68498495 None:intergenic
CATTGAAAGTCAATCTTTCA+TGG 0.320633 4.3:-68498402 MS.gene060730:CDS
TACATTCAGTAATGAAGAAA+TGG 0.368381 4.3:-68498477 MS.gene060730:CDS
TACCAACCTCCTTCAATTTG+TGG 0.372241 4.3:+68498564 None:intergenic
ATGTTGTCGCCATTATTCAT+AGG 0.380827 4.3:+68498422 None:intergenic
TATGCAGAATAATATGTCAT+TGG 0.399656 4.3:-68498618 MS.gene060730:CDS
GGAAAGCAACATACAATAGA+AGG 0.409123 4.3:-68498946 MS.gene060730:CDS
TAATGAAGAAATGGCAATGA+TGG 0.426450 4.3:-68498468 MS.gene060730:CDS
GGAGATGAGTCGGTGCCATT+TGG 0.429830 4.3:-68498359 MS.gene060730:CDS
TGTGGTGACGGCCAGATCTC+TGG 0.431268 4.3:+68498833 None:intergenic
AATTGAAGGAGGTTGGTAGA+AGG 0.442772 4.3:-68498559 MS.gene060730:CDS
GAAGGAGGTTGGTAGAAGGT+TGG 0.444882 4.3:-68498555 MS.gene060730:CDS
GGAATGCCGCCTATGAATAA+TGG 0.453131 4.3:-68498431 MS.gene060730:CDS
CATGAAAGAAAGGGTTCTAA+AGG 0.457088 4.3:-68498729 MS.gene060730:CDS
TGGTAGAAGGTTGGAAGCAG+AGG 0.457415 4.3:-68498546 MS.gene060730:CDS
GTTGATTGACCACAAATTGA+AGG 0.458305 4.3:-68498573 MS.gene060730:CDS
AGAGATCTGGCCGTCACCAC+AGG 0.458628 4.3:-68498831 MS.gene060730:CDS
AAGAGACTTACATGAAAGAA+AGG 0.463147 4.3:-68498739 MS.gene060730:CDS
ATCTCTGGTTGAGGCTCGTA+GGG 0.464949 4.3:+68498848 None:intergenic
TTGATTCAAAACATCTTTGT+TGG 0.470119 4.3:-68498595 MS.gene060730:CDS
ACGAGCCTCAACCAGAGATC+TGG 0.471522 4.3:-68498844 MS.gene060730:CDS
ATTGAAAGTCAATCTTTCAT+GGG 0.502132 4.3:-68498401 MS.gene060730:CDS
CTCGTAGGGTCCATAAACTA+TGG 0.509841 4.3:+68498862 None:intergenic
AGTAGATTTGGTAAAACTCT+AGG 0.510937 4.3:+68498326 None:intergenic
CTAGGATCGACTTCGCCAAA+TGG 0.512642 4.3:+68498344 None:intergenic
GACCACAAATTGAAGGAGGT+TGG 0.522187 4.3:-68498566 MS.gene060730:CDS
GATCTCTGGTTGAGGCTCGT+AGG 0.522551 4.3:+68498847 None:intergenic
GACGGCCAGATCTCTGGTTG+AGG 0.531549 4.3:+68498839 None:intergenic
ATGATGGGTCCTAGCCATGC+TGG 0.537310 4.3:-68498452 MS.gene060730:CDS
AAAATTCAGAACAATGAATG+TGG 0.538041 4.3:-68498789 MS.gene060730:CDS
GCGGAAGGGTCTACTGAAGA+AGG 0.542122 4.3:-68498924 MS.gene060730:CDS
AGAATCATCATCGACAATGA+AGG 0.542377 4.3:+68498970 None:intergenic
AAATTCAGAACAATGAATGT+GGG 0.550450 4.3:-68498788 MS.gene060730:CDS
AAGGCATGATAACAAGGAGA+TGG 0.564923 4.3:-68498684 MS.gene060730:CDS
AACATACAATAGAAGGCGGA+AGG 0.566279 4.3:-68498939 MS.gene060730:CDS
AATGAAGAAATGGCAATGAT+GGG 0.575476 4.3:-68498467 MS.gene060730:CDS
AGAGACTTACATGAAAGAAA+GGG 0.577279 4.3:-68498738 MS.gene060730:CDS
GTTTGGACTCCCTGTGGTGA+CGG 0.587157 4.3:+68498821 None:intergenic
GATACAAAGGCATGATAACA+AGG 0.593660 4.3:-68498690 MS.gene060730:CDS
AAGAGAAACTAAAGATACAA+AGG 0.596350 4.3:-68498703 MS.gene060730:CDS
GGCGGCATTCCAGCATGGCT+AGG 0.600043 4.3:+68498443 None:intergenic
AATTCAGAACAATGAATGTG+GGG 0.603139 4.3:-68498787 MS.gene060730:CDS
GGCTTGATGATGAATAGTAA+TGG 0.618975 4.3:-68498380 MS.gene060730:CDS
TCATAGGCGGCATTCCAGCA+TGG 0.625874 4.3:+68498438 None:intergenic
TTACTGAATGTAAATGGTGG+TGG 0.629493 4.3:+68498488 None:intergenic
GATTGACCACAAATTGAAGG+AGG 0.630849 4.3:-68498570 MS.gene060730:CDS
GAGATCTGGCCGTCACCACA+GGG 0.642312 4.3:-68498830 MS.gene060730:CDS
TTGTCGCCATTATTCATAGG+CGG 0.647988 4.3:+68498425 None:intergenic
ATTCAGAACAATGAATGTGG+GGG 0.650757 4.3:-68498786 MS.gene060730:CDS
GAATAGTAATGGAGATGAGT+CGG 0.650993 4.3:-68498369 MS.gene060730:CDS
AGCACTGTTTGGACTCCCTG+TGG 0.665566 4.3:+68498815 None:intergenic
ACATACAATAGAAGGCGGAA+GGG 0.677825 4.3:-68498938 MS.gene060730:CDS
AAGCAACATACAATAGAAGG+CGG 0.694844 4.3:-68498943 MS.gene060730:CDS
TATGAACTAAGCACTCTGTG+TGG 0.701795 4.3:-68498899 MS.gene060730:CDS
TCATTACTGAATGTAAATGG+TGG 0.709945 4.3:+68498485 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAATTCAGAACAATGAATG+TGG - chr4.3:68498525-68498544 MS.gene060730:CDS 25.0%
! AAATTCAGAACAATGAATGT+GGG - chr4.3:68498526-68498545 MS.gene060730:CDS 25.0%
! AAGAGAAACTAAAGATACAA+AGG - chr4.3:68498611-68498630 MS.gene060730:CDS 25.0%
! ATTGAAAGTCAATCTTTCAT+GGG - chr4.3:68498913-68498932 MS.gene060730:CDS 25.0%
! TACATTCAGTAATGAAGAAA+TGG - chr4.3:68498837-68498856 MS.gene060730:CDS 25.0%
! TATGCAGAATAATATGTCAT+TGG - chr4.3:68498696-68498715 MS.gene060730:CDS 25.0%
! TCTTCATTACTGAATGTAAA+TGG + chr4.3:68498835-68498854 None:intergenic 25.0%
! TTGATTCAAAACATCTTTGT+TGG - chr4.3:68498719-68498738 MS.gene060730:CDS 25.0%
AAGAGACTTACATGAAAGAA+AGG - chr4.3:68498575-68498594 MS.gene060730:CDS 30.0%
AATGAAGAAATGGCAATGAT+GGG - chr4.3:68498847-68498866 MS.gene060730:CDS 30.0%
AATTCAGAACAATGAATGTG+GGG - chr4.3:68498527-68498546 MS.gene060730:CDS 30.0%
AGAGACTTACATGAAAGAAA+GGG - chr4.3:68498576-68498595 MS.gene060730:CDS 30.0%
CATTGAAAGTCAATCTTTCA+TGG - chr4.3:68498912-68498931 MS.gene060730:CDS 30.0%
TAATGAAGAAATGGCAATGA+TGG - chr4.3:68498846-68498865 MS.gene060730:CDS 30.0%
TCATTACTGAATGTAAATGG+TGG + chr4.3:68498832-68498851 None:intergenic 30.0%
! AGTAGATTTGGTAAAACTCT+AGG + chr4.3:68498991-68499010 None:intergenic 30.0%
AAGCAACATACAATAGAAGG+CGG - chr4.3:68498371-68498390 MS.gene060730:CDS 35.0%
AGAATCATCATCGACAATGA+AGG + chr4.3:68498347-68498366 None:intergenic 35.0%
ATGTTGTCGCCATTATTCAT+AGG + chr4.3:68498895-68498914 None:intergenic 35.0%
ATTCAGAACAATGAATGTGG+GGG - chr4.3:68498528-68498547 MS.gene060730:CDS 35.0%
GGAAAGCAACATACAATAGA+AGG - chr4.3:68498368-68498387 MS.gene060730:CDS 35.0%
GTTGATTGACCACAAATTGA+AGG - chr4.3:68498741-68498760 MS.gene060730:CDS 35.0%
TCATTGTCGATGATGATTCT+AGG - chr4.3:68498347-68498366 MS.gene060730:CDS 35.0%
TTACTGAATGTAAATGGTGG+TGG + chr4.3:68498829-68498848 None:intergenic 35.0%
! ATAGCTTGTGCCATAGTTTA+TGG - chr4.3:68498442-68498461 MS.gene060730:CDS 35.0%
! CATGAAAGAAAGGGTTCTAA+AGG - chr4.3:68498585-68498604 MS.gene060730:CDS 35.0%
! GAATAGTAATGGAGATGAGT+CGG - chr4.3:68498945-68498964 MS.gene060730:CDS 35.0%
! GATACAAAGGCATGATAACA+AGG - chr4.3:68498624-68498643 MS.gene060730:CDS 35.0%
!! ATGTAAATGGTGGTGGTATT+TGG + chr4.3:68498822-68498841 None:intergenic 35.0%
!! GGCTTGATGATGAATAGTAA+TGG - chr4.3:68498934-68498953 MS.gene060730:CDS 35.0%
!!! TGAATTTTGAGAGCACTGTT+TGG + chr4.3:68498513-68498532 None:intergenic 35.0%
AACATACAATAGAAGGCGGA+AGG - chr4.3:68498375-68498394 MS.gene060730:CDS 40.0%
AAGGCATGATAACAAGGAGA+TGG - chr4.3:68498630-68498649 MS.gene060730:CDS 40.0%
ACATACAATAGAAGGCGGAA+GGG - chr4.3:68498376-68498395 MS.gene060730:CDS 40.0%
GATTGACCACAAATTGAAGG+AGG - chr4.3:68498744-68498763 MS.gene060730:CDS 40.0%
TACCAACCTCCTTCAATTTG+TGG + chr4.3:68498753-68498772 None:intergenic 40.0%
TATGAACTAAGCACTCTGTG+TGG - chr4.3:68498415-68498434 MS.gene060730:CDS 40.0%
TTGTCGCCATTATTCATAGG+CGG + chr4.3:68498892-68498911 None:intergenic 40.0%
!! AATTGAAGGAGGTTGGTAGA+AGG - chr4.3:68498755-68498774 MS.gene060730:CDS 40.0%
CTCGTAGGGTCCATAAACTA+TGG + chr4.3:68498455-68498474 None:intergenic 45.0%
GACCACAAATTGAAGGAGGT+TGG - chr4.3:68498748-68498767 MS.gene060730:CDS 45.0%
GGAATGCCGCCTATGAATAA+TGG - chr4.3:68498883-68498902 MS.gene060730:CDS 45.0%
ATCTCTGGTTGAGGCTCGTA+GGG + chr4.3:68498469-68498488 None:intergenic 50.0%
GGGGGAGAAGAGCAACAAAA+CGG - chr4.3:68498546-68498565 MS.gene060730:CDS 50.0%
TGGTAGAAGGTTGGAAGCAG+AGG - chr4.3:68498768-68498787 MS.gene060730:CDS 50.0%
! CTAGGATCGACTTCGCCAAA+TGG + chr4.3:68498973-68498992 None:intergenic 50.0%
!! GAAGGAGGTTGGTAGAAGGT+TGG - chr4.3:68498759-68498778 MS.gene060730:CDS 50.0%
ACGAGCCTCAACCAGAGATC+TGG - chr4.3:68498470-68498489 MS.gene060730:CDS 55.0%
AGCACTGTTTGGACTCCCTG+TGG + chr4.3:68498502-68498521 None:intergenic 55.0%
GATCTCTGGTTGAGGCTCGT+AGG + chr4.3:68498470-68498489 None:intergenic 55.0%
GCGGAAGGGTCTACTGAAGA+AGG - chr4.3:68498390-68498409 MS.gene060730:CDS 55.0%
GTTTGGACTCCCTGTGGTGA+CGG + chr4.3:68498496-68498515 None:intergenic 55.0%
TCATAGGCGGCATTCCAGCA+TGG + chr4.3:68498879-68498898 None:intergenic 55.0%
!! ATGATGGGTCCTAGCCATGC+TGG - chr4.3:68498862-68498881 MS.gene060730:CDS 55.0%
!! GGAGATGAGTCGGTGCCATT+TGG - chr4.3:68498955-68498974 MS.gene060730:CDS 55.0%
AGAGATCTGGCCGTCACCAC+AGG - chr4.3:68498483-68498502 MS.gene060730:CDS 60.0%
GACGGCCAGATCTCTGGTTG+AGG + chr4.3:68498478-68498497 None:intergenic 60.0%
GAGATCTGGCCGTCACCACA+GGG - chr4.3:68498484-68498503 MS.gene060730:CDS 60.0%
!! TGTGGTGACGGCCAGATCTC+TGG + chr4.3:68498484-68498503 None:intergenic 60.0%
GGCGGCATTCCAGCATGGCT+AGG + chr4.3:68498874-68498893 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 68498319 68499017 68498319 ID=MS.gene060730
chr4.3 mRNA 68498319 68499017 68498319 ID=MS.gene060730.t1;Parent=MS.gene060730
chr4.3 exon 68498319 68499017 68498319 ID=MS.gene060730.t1.exon1;Parent=MS.gene060730.t1
chr4.3 CDS 68498319 68499017 68498319 ID=cds.MS.gene060730.t1;Parent=MS.gene060730.t1
Gene Sequence

>MS.gene060730

ATGACTAGGAAAAAGGTGAAACTTGCCTTCATTGTCGATGATGATTCTAGGAAAGCAACATACAATAGAAGGCGGAAGGGTCTACTGAAGAAGGTTTATGAACTAAGCACTCTGTGTGGAGTTATAGCTTGTGCCATAGTTTATGGACCCTACGAGCCTCAACCAGAGATCTGGCCGTCACCACAGGGAGTCCAAACAGTGCTCTCAAAATTCAGAACAATGAATGTGGGGGAGAAGAGCAACAAAACGGTTGATCAAGAGACTTACATGAAAGAAAGGGTTCTAAAGGCTAAAGAGAAACTAAAGATACAAAGGCATGATAACAAGGAGATGGAGATGACAATGCTCATGATTCAATGTCTTTTTGACAATAAATTTATGCAGAATAATATGTCATTGGTTGATTCAAAACATCTTTGTTGGTTGATTGACCACAAATTGAAGGAGGTTGGTAGAAGGTTGGAAGCAGAGGATAATAGTAGTCAACATCAAATTCAACTCCAAATACCACCACCATTTACATTCAGTAATGAAGAAATGGCAATGATGGGTCCTAGCCATGCTGGAATGCCGCCTATGAATAATGGCGACAACATTGAAAGTCAATCTTTCATGGGCTTGATGATGAATAGTAATGGAGATGAGTCGGTGCCATTTGGCGAAGTCGATCCTAGAGTTTTACCAAATCTACTACCTTAA
Protein sequence

>MS.gene060730.t1

MTRKKVKLAFIVDDDSRKATYNRRRKGLLKKVYELSTLCGVIACAIVYGPYEPQPEIWPSPQGVQTVLSKFRTMNVGEKSNKTVDQETYMKERVLKAKEKLKIQRHDNKEMEMTMLMIQCLFDNKFMQNNMSLVDSKHLCWLIDHKLKEVGRRLEAEDNSSQHQIQLQIPPPFTFSNEEMAMMGPSHAGMPPMNNGDNIESQSFMGLMMNSNGDESVPFGEVDPRVLPNLLP