Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060805.t1 | XP_024636802.1 | 69.7 | 234 | 69 | 2 | 1 | 234 | 1 | 232 | 2.60E-56 | 228.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060805.t1 | A0A396IJH9 | 69.7 | 234 | 69 | 2 | 1 | 234 | 1 | 232 | 1.9e-56 | 228.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene060805.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060805.t1 | MTR_3g084720 | 47.755 | 245 | 98 | 4 | 1 | 231 | 1 | 229 | 7.57e-61 | 191 |
| MS.gene060805.t1 | MTR_6g465230 | 63.415 | 123 | 40 | 3 | 1 | 120 | 98 | 218 | 9.03e-35 | 124 |
| MS.gene060805.t1 | MTR_7g010470 | 65.000 | 80 | 28 | 0 | 155 | 234 | 1 | 80 | 4.22e-29 | 105 |
| MS.gene060805.t1 | MTR_6g055180 | 54.945 | 91 | 33 | 2 | 1 | 87 | 1 | 87 | 9.07e-19 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060805.t1 | AT2G18490 | 63.158 | 57 | 21 | 0 | 122 | 178 | 155 | 211 | 1.03e-20 | 87.8 |
| MS.gene060805.t1 | AT2G18490 | 63.158 | 57 | 21 | 0 | 122 | 178 | 205 | 261 | 1.68e-20 | 88.2 |
| MS.gene060805.t1 | AT2G15740 | 59.701 | 67 | 23 | 1 | 121 | 187 | 229 | 291 | 5.08e-20 | 87.4 |
| MS.gene060805.t1 | AT5G22990 | 65.079 | 63 | 21 | 1 | 117 | 178 | 227 | 289 | 5.36e-20 | 87.0 |
| MS.gene060805.t1 | AT5G42640 | 42.478 | 113 | 47 | 4 | 78 | 178 | 179 | 285 | 1.06e-19 | 85.9 |
Find 47 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCATTGAGCTTTAGAATC+TGG | 0.282776 | 8.1:-11550532 | None:intergenic |
| GTTTATTGTGGGGTAGACTA+TGG | 0.293623 | 8.1:-11550364 | None:intergenic |
| AACAATATTGTTGCGACTAA+TGG | 0.347097 | 8.1:+11550269 | MS.gene060805:CDS |
| CAAATGCTTAAAACACAGTT+TGG | 0.350424 | 8.1:+11550098 | MS.gene060805:CDS |
| CATCTGATAATAATAATAAT+AGG | 0.353038 | 8.1:+11550144 | MS.gene060805:CDS |
| CGACTAATGGTGATGAAGTT+CGG | 0.362539 | 8.1:+11550282 | MS.gene060805:CDS |
| GACTAATGGTGATGAAGTTC+GGG | 0.387698 | 8.1:+11550283 | MS.gene060805:CDS |
| TCTGGAGATCTGGTCTCTTT+CGG | 0.392995 | 8.1:-11550514 | None:intergenic |
| AGCTTTAGAATCTGGAGATC+TGG | 0.424493 | 8.1:-11550524 | None:intergenic |
| TCAGCCGATAGTAACAATTT+CGG | 0.440639 | 8.1:+11550044 | MS.gene060805:CDS |
| TGTATTGATTTGATGATGCC+TGG | 0.446641 | 8.1:-11550077 | None:intergenic |
| GCTATTACCTTGTTGCACTT+TGG | 0.453373 | 8.1:-11550404 | None:intergenic |
| TTGCACTTTGGACATATGTA+TGG | 0.455080 | 8.1:-11550392 | None:intergenic |
| GACGAGCCTCCAAGGAGATC+AGG | 0.458143 | 8.1:-11550224 | None:intergenic |
| TTATTATTATTATCAGATGA+TGG | 0.465331 | 8.1:-11550140 | None:intergenic |
| ACAATATTGTTTGCCTCAGA+TGG | 0.469502 | 8.1:-11550257 | None:intergenic |
| CAACCACCATGAGTTCTGAT+AGG | 0.471836 | 8.1:+11549982 | None:intergenic |
| TACGCTCATCCTGATCTCCT+TGG | 0.478788 | 8.1:+11550215 | MS.gene060805:CDS |
| ACCCCACTTAGAGGAGGAGC+AGG | 0.481368 | 8.1:-11550641 | None:intergenic |
| TCCCTGCTCCTCCTCTAAGT+GGG | 0.487095 | 8.1:+11550639 | MS.gene060805:CDS |
| AAAGAAGAAATTGTTGATGA+TGG | 0.494936 | 8.1:+11550335 | MS.gene060805:CDS |
| GTCCCTGCTCCTCCTCTAAG+TGG | 0.497357 | 8.1:+11550638 | MS.gene060805:CDS |
| TGTATGGTCCATGTTTATTG+TGG | 0.503637 | 8.1:-11550376 | None:intergenic |
| GTTCTGATAGGTTGATGAAA+TGG | 0.508320 | 8.1:+11549994 | MS.gene060805:CDS |
| TATTGTTTGCCTCAGATGGC+GGG | 0.510054 | 8.1:-11550253 | None:intergenic |
| CAGATTCTAAAGCTCAATGA+TGG | 0.517574 | 8.1:+11550533 | MS.gene060805:CDS |
| AATATATATTAATTATCAGC+AGG | 0.528844 | 8.1:-11550680 | None:intergenic |
| GTATGGTCCATGTTTATTGT+GGG | 0.530171 | 8.1:-11550375 | None:intergenic |
| GCTCATCCTGATCTCCTTGG+AGG | 0.534371 | 8.1:+11550218 | MS.gene060805:CDS |
| CATCAACCTATCAGAACTCA+TGG | 0.543143 | 8.1:-11549988 | None:intergenic |
| CAAATAGTACCAACATGAAG+AGG | 0.557023 | 8.1:+11550309 | MS.gene060805:CDS |
| GGTGTGATGACGAGCCTCCA+AGG | 0.564529 | 8.1:-11550232 | None:intergenic |
| AATTTCGGTAGTCATGCTCC+AGG | 0.573474 | 8.1:+11550059 | MS.gene060805:CDS |
| ACTACCGAAATTGTTACTAT+CGG | 0.574375 | 8.1:-11550048 | None:intergenic |
| CCCTGCTCCTCCTCTAAGTG+GGG | 0.591604 | 8.1:+11550640 | MS.gene060805:CDS |
| ATATTGTTTGCCTCAGATGG+CGG | 0.593923 | 8.1:-11550254 | None:intergenic |
| TGAGTATCATAACTGACAGA+AGG | 0.596005 | 8.1:-11550605 | None:intergenic |
| GTCATCACACCCGCCATCTG+AGG | 0.607113 | 8.1:+11550244 | MS.gene060805:CDS |
| GTTACTATCGGCTGATGATG+TGG | 0.609878 | 8.1:-11550036 | None:intergenic |
| TATGGTCCATGTTTATTGTG+GGG | 0.620016 | 8.1:-11550374 | None:intergenic |
| CATATGTCCAAAGTGCAACA+AGG | 0.620267 | 8.1:+11550397 | MS.gene060805:CDS |
| TGTTATCAACAACAACAAGA+TGG | 0.620498 | 8.1:+11550172 | MS.gene060805:CDS |
| TGATCAGTAGAAGCAATGAC+AGG | 0.628764 | 8.1:-11550572 | None:intergenic |
| TGTATCAAGTCACTACAAGT+CGG | 0.647352 | 8.1:+11550451 | MS.gene060805:CDS |
| AGTCTACCCCACAATAAACA+TGG | 0.648429 | 8.1:+11550368 | MS.gene060805:CDS |
| CCCCACTTAGAGGAGGAGCA+GGG | 0.660323 | 8.1:-11550640 | None:intergenic |
| AGGTTGATGAAATGGTTGTG+TGG | 0.707031 | 8.1:+11550002 | MS.gene060805:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATCTGATAATAATAATAAT+AGG | + | chr8.1:11550144-11550163 | MS.gene060805:CDS | 15.0% |
| !! | TTATTATTATTATCAGATGA+TGG | - | chr8.1:11550143-11550162 | None:intergenic | 15.0% |
| !! | AAAGAAGAAATTGTTGATGA+TGG | + | chr8.1:11550335-11550354 | MS.gene060805:CDS | 25.0% |
| AACAATATTGTTGCGACTAA+TGG | + | chr8.1:11550269-11550288 | MS.gene060805:CDS | 30.0% | |
| ACTACCGAAATTGTTACTAT+CGG | - | chr8.1:11550051-11550070 | None:intergenic | 30.0% | |
| CAAATGCTTAAAACACAGTT+TGG | + | chr8.1:11550098-11550117 | MS.gene060805:CDS | 30.0% | |
| GAAAAAAGAGATACATGTCT+AGG | + | chr8.1:11550489-11550508 | MS.gene060805:CDS | 30.0% | |
| TGTTATCAACAACAACAAGA+TGG | + | chr8.1:11550172-11550191 | MS.gene060805:CDS | 30.0% | |
| !!! | TTCTTTTTTCCTCTTCATGT+TGG | - | chr8.1:11550321-11550340 | None:intergenic | 30.0% |
| ACAATATTGTTTGCCTCAGA+TGG | - | chr8.1:11550260-11550279 | None:intergenic | 35.0% | |
| CAAATAGTACCAACATGAAG+AGG | + | chr8.1:11550309-11550328 | MS.gene060805:CDS | 35.0% | |
| GTATGGTCCATGTTTATTGT+GGG | - | chr8.1:11550378-11550397 | None:intergenic | 35.0% | |
| TATGGTCCATGTTTATTGTG+GGG | - | chr8.1:11550377-11550396 | None:intergenic | 35.0% | |
| TCAGCCGATAGTAACAATTT+CGG | + | chr8.1:11550044-11550063 | MS.gene060805:CDS | 35.0% | |
| TGAGTATCATAACTGACAGA+AGG | - | chr8.1:11550608-11550627 | None:intergenic | 35.0% | |
| TGTATCAAGTCACTACAAGT+CGG | + | chr8.1:11550451-11550470 | MS.gene060805:CDS | 35.0% | |
| TGTATGGTCCATGTTTATTG+TGG | - | chr8.1:11550379-11550398 | None:intergenic | 35.0% | |
| TTGCACTTTGGACATATGTA+TGG | - | chr8.1:11550395-11550414 | None:intergenic | 35.0% | |
| ! | TTAACTTTTACCCCACTTAG+AGG | - | chr8.1:11550653-11550672 | None:intergenic | 35.0% |
| !! | CAGATTCTAAAGCTCAATGA+TGG | + | chr8.1:11550533-11550552 | MS.gene060805:CDS | 35.0% |
| !! | CATCATTGAGCTTTAGAATC+TGG | - | chr8.1:11550535-11550554 | None:intergenic | 35.0% |
| !! | GTTCTGATAGGTTGATGAAA+TGG | + | chr8.1:11549994-11550013 | MS.gene060805:CDS | 35.0% |
| !! | TGTATTGATTTGATGATGCC+TGG | - | chr8.1:11550080-11550099 | None:intergenic | 35.0% |
| AGTCTACCCCACAATAAACA+TGG | + | chr8.1:11550368-11550387 | MS.gene060805:CDS | 40.0% | |
| ATATTGTTTGCCTCAGATGG+CGG | - | chr8.1:11550257-11550276 | None:intergenic | 40.0% | |
| CATATGTCCAAAGTGCAACA+AGG | + | chr8.1:11550397-11550416 | MS.gene060805:CDS | 40.0% | |
| GCTATTACCTTGTTGCACTT+TGG | - | chr8.1:11550407-11550426 | None:intergenic | 40.0% | |
| GTTTATTGTGGGGTAGACTA+TGG | - | chr8.1:11550367-11550386 | None:intergenic | 40.0% | |
| TGATCAGTAGAAGCAATGAC+AGG | - | chr8.1:11550575-11550594 | None:intergenic | 40.0% | |
| ! | CGACTAATGGTGATGAAGTT+CGG | + | chr8.1:11550282-11550301 | MS.gene060805:CDS | 40.0% |
| ! | GACTAATGGTGATGAAGTTC+GGG | + | chr8.1:11550283-11550302 | MS.gene060805:CDS | 40.0% |
| !! | AGCTTTAGAATCTGGAGATC+TGG | - | chr8.1:11550527-11550546 | None:intergenic | 40.0% |
| !! | AGGTTGATGAAATGGTTGTG+TGG | + | chr8.1:11550002-11550021 | MS.gene060805:CDS | 40.0% |
| AATTTCGGTAGTCATGCTCC+AGG | + | chr8.1:11550059-11550078 | MS.gene060805:CDS | 45.0% | |
| TATTGTTTGCCTCAGATGGC+GGG | - | chr8.1:11550256-11550275 | None:intergenic | 45.0% | |
| TCTGGAGATCTGGTCTCTTT+CGG | - | chr8.1:11550517-11550536 | None:intergenic | 45.0% | |
| ! | ACTTTTACCCCACTTAGAGG+AGG | - | chr8.1:11550650-11550669 | None:intergenic | 45.0% |
| ! | GTTACTATCGGCTGATGATG+TGG | - | chr8.1:11550039-11550058 | None:intergenic | 45.0% |
| TACGCTCATCCTGATCTCCT+TGG | + | chr8.1:11550215-11550234 | MS.gene060805:CDS | 50.0% | |
| GCTCATCCTGATCTCCTTGG+AGG | + | chr8.1:11550218-11550237 | MS.gene060805:CDS | 55.0% | |
| TCCCTGCTCCTCCTCTAAGT+GGG | + | chr8.1:11550639-11550658 | MS.gene060805:CDS | 55.0% | |
| ACCCCACTTAGAGGAGGAGC+AGG | - | chr8.1:11550644-11550663 | None:intergenic | 60.0% | |
| CCCCACTTAGAGGAGGAGCA+GGG | - | chr8.1:11550643-11550662 | None:intergenic | 60.0% | |
| CCCTGCTCCTCCTCTAAGTG+GGG | + | chr8.1:11550640-11550659 | MS.gene060805:CDS | 60.0% | |
| GACGAGCCTCCAAGGAGATC+AGG | - | chr8.1:11550227-11550246 | None:intergenic | 60.0% | |
| GTCATCACACCCGCCATCTG+AGG | + | chr8.1:11550244-11550263 | MS.gene060805:CDS | 60.0% | |
| GTCCCTGCTCCTCCTCTAAG+TGG | + | chr8.1:11550638-11550657 | MS.gene060805:CDS | 60.0% | |
| ! | GGTGTGATGACGAGCCTCCA+AGG | - | chr8.1:11550235-11550254 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 11549990 | 11550694 | 11549990 | ID=MS.gene060805 |
| chr8.1 | mRNA | 11549990 | 11550694 | 11549990 | ID=MS.gene060805.t1;Parent=MS.gene060805 |
| chr8.1 | exon | 11549990 | 11550694 | 11549990 | ID=MS.gene060805.t1.exon1;Parent=MS.gene060805.t1 |
| chr8.1 | CDS | 11549990 | 11550694 | 11549990 | ID=cds.MS.gene060805.t1;Parent=MS.gene060805.t1 |
| Gene Sequence |
| Protein sequence |
