Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061011.t1 | KEH40705.1 | 93.4 | 91 | 6 | 0 | 1 | 91 | 3 | 93 | 4.40E-40 | 173.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061011.t1 | Q9FNN6 | 58.8 | 85 | 34 | 1 | 1 | 85 | 3 | 86 | 2.3e-21 | 102.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061011.t1 | A0A072VRE4 | 93.4 | 91 | 6 | 0 | 1 | 91 | 3 | 93 | 3.2e-40 | 173.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene061011.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061011.t1 | MTR_1g033600 | 93.182 | 88 | 6 | 0 | 1 | 88 | 3 | 90 | 3.87e-55 | 168 |
| MS.gene061011.t1 | MTR_8g063600 | 67.742 | 93 | 29 | 1 | 1 | 93 | 1 | 92 | 8.98e-35 | 120 |
| MS.gene061011.t1 | MTR_8g063870 | 71.053 | 76 | 20 | 1 | 1 | 76 | 1 | 74 | 3.50e-29 | 105 |
| MS.gene061011.t1 | MTR_1g022290 | 51.807 | 83 | 40 | 0 | 4 | 86 | 1 | 83 | 1.84e-27 | 96.7 |
| MS.gene061011.t1 | MTR_3g111880 | 51.250 | 80 | 37 | 1 | 7 | 86 | 4 | 81 | 3.23e-22 | 83.2 |
| MS.gene061011.t1 | MTR_7g089210 | 55.714 | 70 | 28 | 1 | 13 | 82 | 15 | 81 | 1.55e-21 | 81.3 |
| MS.gene061011.t1 | MTR_3g116720 | 51.471 | 68 | 33 | 0 | 8 | 75 | 14 | 81 | 1.90e-21 | 80.9 |
| MS.gene061011.t1 | MTR_5g020170 | 52.174 | 69 | 33 | 0 | 5 | 73 | 14 | 82 | 6.27e-21 | 80.1 |
| MS.gene061011.t1 | MTR_6g004250 | 51.389 | 72 | 35 | 0 | 1 | 72 | 1 | 72 | 3.68e-20 | 77.8 |
| MS.gene061011.t1 | MTR_4g111975 | 50.000 | 80 | 35 | 2 | 10 | 86 | 8 | 85 | 4.42e-20 | 82.4 |
| MS.gene061011.t1 | MTR_1g111830 | 54.167 | 72 | 33 | 0 | 8 | 79 | 22 | 93 | 9.09e-20 | 80.9 |
| MS.gene061011.t1 | MTR_3g111920 | 37.179 | 78 | 49 | 0 | 10 | 87 | 3 | 80 | 8.08e-19 | 74.3 |
| MS.gene061011.t1 | MTR_5g081860 | 52.174 | 69 | 33 | 0 | 8 | 76 | 25 | 93 | 1.09e-18 | 78.2 |
| MS.gene061011.t1 | MTR_3g462790 | 36.364 | 99 | 56 | 1 | 2 | 93 | 35 | 133 | 1.14e-18 | 79.0 |
| MS.gene061011.t1 | MTR_7g067080 | 41.837 | 98 | 49 | 2 | 5 | 94 | 30 | 127 | 3.20e-18 | 77.8 |
| MS.gene061011.t1 | MTR_3g013440 | 45.333 | 75 | 40 | 1 | 1 | 74 | 1 | 75 | 3.50e-17 | 70.5 |
| MS.gene061011.t1 | MTR_8g077360 | 41.429 | 70 | 40 | 1 | 7 | 76 | 2 | 70 | 4.93e-17 | 69.7 |
| MS.gene061011.t1 | MTR_8g077380 | 41.429 | 70 | 40 | 1 | 7 | 76 | 2 | 70 | 5.48e-17 | 69.3 |
| MS.gene061011.t1 | MTR_8g077390 | 41.429 | 70 | 40 | 1 | 7 | 76 | 2 | 70 | 5.48e-17 | 69.3 |
| MS.gene061011.t1 | MTR_5g069710 | 40.000 | 95 | 51 | 2 | 7 | 95 | 26 | 120 | 1.57e-16 | 72.8 |
| MS.gene061011.t1 | MTR_8g077420 | 40.580 | 69 | 39 | 2 | 8 | 76 | 3 | 69 | 2.67e-14 | 62.4 |
| MS.gene061011.t1 | MTR_5g020540 | 46.032 | 63 | 33 | 1 | 8 | 70 | 2 | 63 | 7.20e-14 | 61.2 |
| MS.gene061011.t1 | MTR_1g086180 | 45.614 | 57 | 30 | 1 | 10 | 65 | 11 | 67 | 1.82e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061011.t1 | AT5G08520 | 59.184 | 98 | 38 | 2 | 1 | 98 | 3 | 98 | 2.17e-32 | 114 |
| MS.gene061011.t1 | AT4G39250 | 53.247 | 77 | 36 | 0 | 10 | 86 | 14 | 90 | 1.59e-25 | 92.0 |
| MS.gene061011.t1 | AT1G75250 | 53.731 | 67 | 31 | 0 | 8 | 74 | 10 | 76 | 6.65e-23 | 85.1 |
| MS.gene061011.t1 | AT1G75250 | 53.731 | 67 | 31 | 0 | 8 | 74 | 10 | 76 | 1.31e-22 | 85.1 |
| MS.gene061011.t1 | AT2G21650 | 53.731 | 67 | 31 | 0 | 10 | 76 | 14 | 80 | 1.97e-20 | 79.0 |
| MS.gene061011.t1 | AT2G18328 | 51.562 | 64 | 31 | 0 | 13 | 76 | 14 | 77 | 2.10e-19 | 75.9 |
| MS.gene061011.t1 | AT1G19510 | 45.946 | 74 | 40 | 0 | 13 | 86 | 15 | 88 | 1.43e-18 | 74.3 |
| MS.gene061011.t1 | AT2G38090 | 39.362 | 94 | 57 | 0 | 5 | 98 | 24 | 117 | 1.92e-18 | 78.2 |
| MS.gene061011.t1 | AT5G05790 | 50.000 | 68 | 34 | 0 | 7 | 74 | 28 | 95 | 2.37e-18 | 77.4 |
| MS.gene061011.t1 | AT5G05790 | 50.000 | 68 | 34 | 0 | 7 | 74 | 28 | 95 | 2.37e-18 | 77.4 |
| MS.gene061011.t1 | AT5G58900 | 47.059 | 68 | 36 | 0 | 8 | 75 | 32 | 99 | 1.59e-17 | 75.5 |
| MS.gene061011.t1 | AT5G23650 | 50.000 | 70 | 35 | 0 | 3 | 72 | 6 | 75 | 2.68e-17 | 75.5 |
| MS.gene061011.t1 | AT3G11280 | 47.761 | 67 | 35 | 0 | 10 | 76 | 33 | 99 | 5.84e-17 | 73.6 |
| MS.gene061011.t1 | AT3G11280 | 47.761 | 67 | 35 | 0 | 10 | 76 | 33 | 99 | 5.84e-17 | 73.6 |
| MS.gene061011.t1 | AT1G49010 | 47.059 | 68 | 33 | 1 | 8 | 72 | 6 | 73 | 2.05e-15 | 70.1 |
| MS.gene061011.t1 | AT5G04760 | 45.455 | 66 | 35 | 1 | 7 | 72 | 3 | 67 | 1.93e-14 | 66.2 |
| MS.gene061011.t1 | AT5G01200 | 39.175 | 97 | 47 | 3 | 8 | 92 | 28 | 124 | 1.96e-12 | 61.6 |
Find 0 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACCATCGTATAAATCTTCTT+CGG | + | 11123:12520-12539 | MS.gene061011:CDS | 30.0% | |
| GAAGATTTATACGATGGTAA+AGG | - | 11123:12518-12537 | MS.gene061011:intergenic | 30.0% | |
| ATCGTATAAATCTTCTTCGG+AGG | + | 11123:12523-12542 | MS.gene061011:CDS | 35.0% | |
| TCCGAAGAAGATTTATACGA+TGG | - | 11123:12524-12543 | MS.gene061011:intergenic | 35.0% | |
| ! | TAAGGCATTTGAAAATGCCT+TGG | + | 11123:12358-12377 | MS.gene061011:CDS | 35.0% |
| GACTTATCCTGAGGATGATT+CGG | + | 11123:12382-12401 | MS.gene061011:CDS | 40.0% | |
| GATATTAGCCAGATTGAAGC+TGG | + | 11123:12485-12504 | MS.gene061011:CDS | 40.0% | |
| TAAGCAGCATTATGAGCTCT+TGG | + | 11123:12457-12476 | MS.gene061011:CDS | 40.0% | |
| ACAAGTCATGCTAGCGATGA+AGG | + | 11123:12551-12570 | MS.gene061011:CDS | 45.0% | |
| ATGCTAGCGATGAAGGAGTT+GGG | + | 11123:12558-12577 | MS.gene061011:CDS | 45.0% | |
| ATTTCTTCCGTAGTCTTCCC+CGG | - | 11123:12437-12456 | MS.gene061011:intergenic | 45.0% | |
| ! | TGAGGATGATTCGGATCGAT+GGG | + | 11123:12391-12410 | MS.gene061011:CDS | 45.0% |
| !! | AAGTGGACAATAGCTCTGAG+TGG | + | 11123:12321-12340 | MS.gene061011:CDS | 45.0% |
| ATCCTCAGGATAAGTCGCCA+AGG | - | 11123:12378-12397 | MS.gene061011:intergenic | 50.0% | |
| CAATGGCCACAAAAACGACG+AGG | + | 11123:12598-12617 | MS.gene061011:CDS | 50.0% | |
| CATCGATCCGAATCATCCTC+AGG | - | 11123:12392-12411 | MS.gene061011:intergenic | 50.0% | |
| CATGCTAGCGATGAAGGAGT+TGG | + | 11123:12557-12576 | MS.gene061011:CDS | 50.0% | |
| GCACACAACCAGCTTCAATC+TGG | - | 11123:12496-12515 | MS.gene061011:intergenic | 50.0% | |
| GGATCGATGGGAGAAGATTG+CGG | + | 11123:12403-12422 | MS.gene061011:CDS | 50.0% | |
| TGCTAGCGATGAAGGAGTTG+GGG | + | 11123:12559-12578 | MS.gene061011:CDS | 50.0% | |
| ! | CTGAGGATGATTCGGATCGA+TGG | + | 11123:12390-12409 | MS.gene061011:CDS | 50.0% |
| !! | ACAATAGCTCTGAGTGGAGC+TGG | + | 11123:12327-12346 | MS.gene061011:CDS | 50.0% |
| !! | CAATAGCTCTGAGTGGAGCT+GGG | + | 11123:12328-12347 | MS.gene061011:CDS | 50.0% |
| AAGATTGCGGCCGATGTACC+GGG | + | 11123:12416-12435 | MS.gene061011:CDS | 55.0% | |
| CGATGTACCGGGGAAGACTA+CGG | + | 11123:12427-12446 | MS.gene061011:CDS | 55.0% | |
| CGTAGTCTTCCCCGGTACAT+CGG | - | 11123:12429-12448 | MS.gene061011:intergenic | 55.0% | |
| CTAGCGATGAAGGAGTTGGG+GGG | + | 11123:12561-12580 | MS.gene061011:CDS | 55.0% | |
| GAAGATTGCGGCCGATGTAC+CGG | + | 11123:12415-12434 | MS.gene061011:CDS | 55.0% | |
| GAAGGAGTTGGGGGGAAGAA+GGG | + | 11123:12569-12588 | MS.gene061011:CDS | 55.0% | |
| GCTAGCGATGAAGGAGTTGG+GGG | + | 11123:12560-12579 | MS.gene061011:CDS | 55.0% | |
| GGGAAGAAGGGTGGTCTCAA+TGG | + | 11123:12581-12600 | MS.gene061011:CDS | 55.0% | |
| GTGGAGCTGGGAGCAAGATA+AGG | + | 11123:12340-12359 | MS.gene061011:CDS | 55.0% | |
| TGAAGGAGTTGGGGGGAAGA+AGG | + | 11123:12568-12587 | MS.gene061011:CDS | 55.0% | |
| TGCCTTGGCGACTTATCCTG+AGG | + | 11123:12373-12392 | MS.gene061011:CDS | 55.0% | |
| AGATTGCGGCCGATGTACCG+GGG | + | 11123:12417-12436 | MS.gene061011:CDS | 60.0% | |
| GGAGTTGGGGGGAAGAAGGG+TGG | + | 11123:12572-12591 | MS.gene061011:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 11123 | gene | 12314 | 12625 | 12314 | ID=MS.gene061011 |
| 11123 | mRNA | 12314 | 12625 | 12314 | ID=MS.gene061011.t1;Parent=MS.gene061011 |
| 11123 | exon | 12314 | 12625 | 12314 | ID=MS.gene061011.t1.exon1;Parent=MS.gene061011.t1 |
| 11123 | CDS | 12314 | 12625 | 12314 | ID=cds.MS.gene061011.t1;Parent=MS.gene061011.t1 |
| Gene Sequence |
| Protein sequence |
