Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061033.t1 | XP_024630307.1 | 76.1 | 201 | 37 | 2 | 1 | 192 | 151 | 349 | 4.60E-75 | 290.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061033.t1 | Q8L3W1 | 33.3 | 102 | 64 | 4 | 22 | 122 | 241 | 339 | 1.3e-06 | 54.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061033.t1 | A0A396JL93 | 87.7 | 130 | 16 | 0 | 1 | 130 | 1 | 130 | 6.9e-57 | 229.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene061033.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061033.t1 | MTR_1g035640 | 50.388 | 129 | 64 | 0 | 2 | 130 | 163 | 291 | 1.06e-35 | 130 |
| MS.gene061033.t1 | MTR_1g035570 | 40.764 | 157 | 89 | 2 | 1 | 155 | 167 | 321 | 4.27e-33 | 122 |
| MS.gene061033.t1 | MTR_1g035620 | 45.198 | 177 | 69 | 6 | 1 | 152 | 160 | 333 | 4.75e-32 | 119 |
| MS.gene061033.t1 | MTR_6g061070 | 46.324 | 136 | 66 | 3 | 3 | 131 | 199 | 334 | 2.08e-29 | 113 |
| MS.gene061033.t1 | MTR_1g050845 | 44.444 | 162 | 66 | 4 | 15 | 152 | 159 | 320 | 3.31e-29 | 114 |
| MS.gene061033.t1 | MTR_1g035490 | 36.066 | 183 | 86 | 4 | 1 | 160 | 86 | 260 | 5.24e-28 | 107 |
| MS.gene061033.t1 | MTR_5g044670 | 40.179 | 112 | 64 | 2 | 22 | 132 | 207 | 316 | 1.98e-20 | 87.0 |
| MS.gene061033.t1 | MTR_8g103940 | 41.176 | 119 | 60 | 5 | 10 | 125 | 226 | 337 | 6.84e-15 | 72.4 |
| MS.gene061033.t1 | MTR_8g103940 | 32.787 | 122 | 74 | 3 | 5 | 120 | 377 | 496 | 8.48e-11 | 60.5 |
| MS.gene061033.t1 | MTR_1g108780 | 36.752 | 117 | 64 | 5 | 10 | 123 | 185 | 294 | 6.03e-13 | 66.6 |
| MS.gene061033.t1 | MTR_1g035540 | 48.485 | 66 | 32 | 1 | 85 | 150 | 32 | 95 | 9.48e-13 | 62.8 |
| MS.gene061033.t1 | MTR_1g035460 | 29.412 | 102 | 66 | 1 | 22 | 123 | 196 | 291 | 1.49e-11 | 62.4 |
| MS.gene061033.t1 | MTR_3g098790 | 32.039 | 103 | 65 | 3 | 22 | 119 | 197 | 299 | 3.67e-11 | 61.2 |
| MS.gene061033.t1 | MTR_1g034240 | 26.144 | 153 | 97 | 4 | 2 | 141 | 269 | 418 | 3.92e-11 | 61.2 |
| MS.gene061033.t1 | MTR_4g068320 | 29.412 | 102 | 70 | 1 | 32 | 133 | 303 | 402 | 8.19e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCGAATAGTGTTTCTTTCT+TGG | 0.151788 | 1.4:+18410522 | None:intergenic |
| TCGCCATTGATCTTTCTTTA+TGG | 0.185876 | 1.4:+18410598 | None:intergenic |
| TCAAAGGGATTGCAAGCATT+AGG | 0.348097 | 1.4:-18411962 | MS.gene061033:CDS |
| CAAAGGGATTGCAAGCATTA+GGG | 0.352259 | 1.4:-18411961 | MS.gene061033:CDS |
| AAAACTTGGATTCTTATCAT+TGG | 0.358112 | 1.4:+18412140 | None:intergenic |
| TATTCTCTTGAAAACTTACT+TGG | 0.379463 | 1.4:+18411996 | None:intergenic |
| TGCTGCTAGGACCAAAGATA+TGG | 0.419607 | 1.4:-18410564 | MS.gene061033:CDS |
| GCAGTCCCTATCCATATCTT+TGG | 0.424950 | 1.4:+18410553 | None:intergenic |
| GTCAATTTAAGTTCAAAACT+TGG | 0.433190 | 1.4:+18412126 | None:intergenic |
| CATTGATCTTTCTTTATGGC+GGG | 0.447376 | 1.4:+18410602 | None:intergenic |
| GGCTTGATTCCTCTCTAGCT+CGG | 0.457584 | 1.4:+18411766 | None:intergenic |
| AATACTTGAACGAGTTCAAA+GGG | 0.491876 | 1.4:-18411977 | MS.gene061033:CDS |
| CTTTGAGAATTCCTTGTACC+CGG | 0.517220 | 1.4:-18411569 | MS.gene061033:intron |
| AGTTAATCATACCGGGTACA+AGG | 0.518064 | 1.4:+18411558 | None:intergenic |
| CCATTGATCTTTCTTTATGG+CGG | 0.521877 | 1.4:+18410601 | None:intergenic |
| TTTAGAAGTTTCTAATGTGA+GGG | 0.522348 | 1.4:-18411611 | MS.gene061033:CDS |
| CTAGGACCAAAGATATGGAT+AGG | 0.523459 | 1.4:-18410559 | MS.gene061033:CDS |
| GTTCTATTGTGAATGGTGGT+TGG | 0.525249 | 1.4:-18411884 | MS.gene061033:CDS |
| CAAAGGAGGCATGACTAAGA+AGG | 0.530395 | 1.4:-18412202 | None:intergenic |
| GCTTGATTCCTCTCTAGCTC+GGG | 0.531379 | 1.4:+18411767 | None:intergenic |
| GGGTTGATGAAGATAGAACT+TGG | 0.534209 | 1.4:-18411941 | MS.gene061033:CDS |
| TCTTTGGTCCTAGCAGCAAT+CGG | 0.535539 | 1.4:+18410569 | None:intergenic |
| TTCTATTTCCCGAGCTAGAG+AGG | 0.537691 | 1.4:-18411775 | MS.gene061033:CDS |
| TGATGAAGATAGAACTTGGA+AGG | 0.543162 | 1.4:-18411937 | MS.gene061033:CDS |
| ATAGAAATATTAAAAGAAAG+AGG | 0.543614 | 1.4:+18411792 | None:intergenic |
| ATTTAGAAGTTTCTAATGTG+AGG | 0.550511 | 1.4:-18411612 | MS.gene061033:CDS |
| GAATACTTGAACGAGTTCAA+AGG | 0.559537 | 1.4:-18411978 | MS.gene061033:CDS |
| TGATCTTTCTTTATGGCGGG+TGG | 0.564206 | 1.4:+18410605 | None:intergenic |
| AACAGAAGTTCTATTGTGAA+TGG | 0.573160 | 1.4:-18411891 | MS.gene061033:CDS |
| CCGCCATAAAGAAAGATCAA+TGG | 0.584531 | 1.4:-18410601 | MS.gene061033:CDS |
| GATCAGAGCCGATTGCTGCT+AGG | 0.587981 | 1.4:-18410577 | MS.gene061033:CDS |
| TAGGACCAAAGATATGGATA+GGG | 0.593044 | 1.4:-18410558 | MS.gene061033:CDS |
| TGGTTGGAATTTATTTACCA+AGG | 0.603797 | 1.4:-18411868 | MS.gene061033:CDS |
| AGAAGTTCTATTGTGAATGG+TGG | 0.647616 | 1.4:-18411888 | MS.gene061033:CDS |
| AAGGAAAATAACTTGCAAGT+CGG | 0.656533 | 1.4:-18411849 | MS.gene061033:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATGACATTTTTTTTTTTTT+TGG | - | chr1.4:18411281-18411300 | MS.gene061033:intron | 10.0% |
| !! | ATAGAAATATTAAAAGAAAG+AGG | + | chr1.4:18410914-18410933 | None:intergenic | 15.0% |
| !!! | TTCTTTTTCATTTCATTTTT+TGG | + | chr1.4:18411942-18411961 | None:intergenic | 15.0% |
| !!! | TTTGAAAATGTCTATTATTT+TGG | - | chr1.4:18410639-18410658 | MS.gene061033:intron | 15.0% |
| !! | CAAAATTTCAAAAGAATAGA+GGG | - | chr1.4:18411756-18411775 | MS.gene061033:CDS | 20.0% |
| !! | GTAAAAATAAAACAACTTGT+TGG | - | chr1.4:18411820-18411839 | MS.gene061033:CDS | 20.0% |
| !! | TGTTTGTTTATTAAAGTTTG+TGG | - | chr1.4:18410997-18411016 | MS.gene061033:intron | 20.0% |
| !!! | AACTTACAAATAATTTTCCT+TGG | + | chr1.4:18410615-18410634 | None:intergenic | 20.0% |
| !!! | ACTTACAAATAATTTTCCTT+GGG | + | chr1.4:18410614-18410633 | None:intergenic | 20.0% |
| !!! | ATATGTTTGATTTATTTGAG+GGG | - | chr1.4:18411913-18411932 | MS.gene061033:CDS | 20.0% |
| !!! | CTCTATTCTTTTGAAATTTT+GGG | + | chr1.4:18411757-18411776 | None:intergenic | 20.0% |
| !!! | GATATGTTTGATTTATTTGA+GGG | - | chr1.4:18411912-18411931 | MS.gene061033:CDS | 20.0% |
| !!! | TATTAAAGTTTGTGGTTTTT+TGG | - | chr1.4:18411005-18411024 | MS.gene061033:intron | 20.0% |
| !!! | TGATATGTTTGATTTATTTG+AGG | - | chr1.4:18411911-18411930 | MS.gene061033:CDS | 20.0% |
| ! | AAAACTTGGATTCTTATCAT+TGG | + | chr1.4:18410566-18410585 | None:intergenic | 25.0% |
| ! | AAACTTCTAAATCTGTAGAA+GGG | + | chr1.4:18411083-18411102 | None:intergenic | 25.0% |
| ! | ATACCGAATGGTAATTAAAT+AGG | + | chr1.4:18411658-18411677 | None:intergenic | 25.0% |
| ! | ATGTAATTCCAAGTTATAGT+TGG | + | chr1.4:18411890-18411909 | None:intergenic | 25.0% |
| ! | ATTAACTATTACGCAAATTG+TGG | - | chr1.4:18411161-18411180 | MS.gene061033:intron | 25.0% |
| ! | ATTTAGAAGTTTCTAATGTG+AGG | - | chr1.4:18411091-18411110 | MS.gene061033:intron | 25.0% |
| ! | CCAAAATTTCAAAAGAATAG+AGG | - | chr1.4:18411755-18411774 | MS.gene061033:CDS | 25.0% |
| ! | GATTAAGAAAGAATGCAAAT+TGG | - | chr1.4:18411420-18411439 | MS.gene061033:intron | 25.0% |
| ! | TACCGAATGGTAATTAAATA+GGG | + | chr1.4:18411657-18411676 | None:intergenic | 25.0% |
| ! | TATTCTCTTGAAAACTTACT+TGG | + | chr1.4:18410710-18410729 | None:intergenic | 25.0% |
| ! | TTGAATATCCAACTATAACT+TGG | - | chr1.4:18411879-18411898 | MS.gene061033:CDS | 25.0% |
| ! | TTGGATATTCAATTATGACT+TGG | + | chr1.4:18411871-18411890 | None:intergenic | 25.0% |
| ! | TTTAGAAGTTTCTAATGTGA+GGG | - | chr1.4:18411092-18411111 | MS.gene061033:intron | 25.0% |
| !! | GTCAATTTAAGTTCAAAACT+TGG | + | chr1.4:18410580-18410599 | None:intergenic | 25.0% |
| !! | TGTTTCTTGTTGATAAGTTT+AGG | - | chr1.4:18411682-18411701 | MS.gene061033:intron | 25.0% |
| !!! | AAGTAACCTTGCAATTTTTT+AGG | + | chr1.4:18410962-18410981 | None:intergenic | 25.0% |
| !!! | AGTAACCTTGCAATTTTTTA+GGG | + | chr1.4:18410961-18410980 | None:intergenic | 25.0% |
| !!! | CCTCTATTCTTTTGAAATTT+TGG | + | chr1.4:18411758-18411777 | None:intergenic | 25.0% |
| AACAGAAGTTCTATTGTGAA+TGG | - | chr1.4:18410812-18410831 | MS.gene061033:intron | 30.0% | |
| AATACTTGAACGAGTTCAAA+GGG | - | chr1.4:18410726-18410745 | MS.gene061033:intron | 30.0% | |
| AGCAGCATCATAATAATCTT+TGG | - | chr1.4:18411598-18411617 | MS.gene061033:CDS | 30.0% | |
| ATCGAATAGTGTTTCTTTCT+TGG | + | chr1.4:18412184-18412203 | None:intergenic | 30.0% | |
| CCAATCTACTATTACTACAA+TGG | + | chr1.4:18412001-18412020 | None:intergenic | 30.0% | |
| CCATTGTAGTAATAGTAGAT+TGG | - | chr1.4:18411998-18412017 | MS.gene061033:CDS | 30.0% | |
| GAAACTTCTAAATCTGTAGA+AGG | + | chr1.4:18411084-18411103 | None:intergenic | 30.0% | |
| GTATTCTCTGTTTCAATTCA+TGG | + | chr1.4:18410688-18410707 | None:intergenic | 30.0% | |
| TAGATAGATGGATGTATGAA+TGG | - | chr1.4:18411216-18411235 | MS.gene061033:intron | 30.0% | |
| TGCCCTATTTAATTACCATT+CGG | - | chr1.4:18411652-18411671 | MS.gene061033:intron | 30.0% | |
| TGCGTAATAGTTAATCATAC+CGG | + | chr1.4:18411156-18411175 | None:intergenic | 30.0% | |
| TGGTTGGAATTTATTTACCA+AGG | - | chr1.4:18410835-18410854 | MS.gene061033:intron | 30.0% | |
| ! | AAGGAAAATAACTTGCAAGT+CGG | - | chr1.4:18410854-18410873 | MS.gene061033:intron | 30.0% |
| !! | ATAAAACAACTTGTTGGTGA+AGG | - | chr1.4:18411826-18411845 | MS.gene061033:CDS | 30.0% |
| !!! | ACCTGATGATTTTTTTGAGT+AGG | - | chr1.4:18411322-18411341 | MS.gene061033:intron | 30.0% |
| AAGAAACATTGCATACCGAA+TGG | + | chr1.4:18411670-18411689 | None:intergenic | 35.0% | |
| AGAAGTTCTATTGTGAATGG+TGG | - | chr1.4:18410815-18410834 | MS.gene061033:intron | 35.0% | |
| AGCATAGCAATAACAAAGCA+TGG | + | chr1.4:18411547-18411566 | None:intergenic | 35.0% | |
| CATTGATCTTTCTTTATGGC+GGG | + | chr1.4:18412104-18412123 | None:intergenic | 35.0% | |
| CCATTGATCTTTCTTTATGG+CGG | + | chr1.4:18412105-18412124 | None:intergenic | 35.0% | |
| GAATACTTGAACGAGTTCAA+AGG | - | chr1.4:18410725-18410744 | MS.gene061033:intron | 35.0% | |
| GCCTACTCAAAAAAATCATC+AGG | + | chr1.4:18411326-18411345 | None:intergenic | 35.0% | |
| GCGTAATAGTTAATCATACC+GGG | + | chr1.4:18411155-18411174 | None:intergenic | 35.0% | |
| TAGATCGATCGATAGATAGA+TGG | - | chr1.4:18411204-18411223 | MS.gene061033:intron | 35.0% | |
| TAGGACCAAAGATATGGATA+GGG | - | chr1.4:18412145-18412164 | MS.gene061033:CDS | 35.0% | |
| TCAAGCCCTAAAAAATTGCA+AGG | - | chr1.4:18410953-18410972 | MS.gene061033:intron | 35.0% | |
| TCGCCATTGATCTTTCTTTA+TGG | + | chr1.4:18412108-18412127 | None:intergenic | 35.0% | |
| TGATGAAGATAGAACTTGGA+AGG | - | chr1.4:18410766-18410785 | MS.gene061033:intron | 35.0% | |
| ! | GAACCAGTTTTTCCCAATAT+TGG | - | chr1.4:18412024-18412043 | MS.gene061033:intron | 35.0% |
| ! | TGATGACCCAATATTTGTTG+CGG | - | chr1.4:18411971-18411990 | MS.gene061033:CDS | 35.0% |
| ! | TTGACACAATTTTATGCCCA+AGG | - | chr1.4:18410595-18410614 | MS.gene061033:CDS | 35.0% |
| !! | GACTTGCAAGTTATTTTCCT+TGG | + | chr1.4:18410855-18410874 | None:intergenic | 35.0% |
| AATATTGGCGATGTGAGCAT+GGG | - | chr1.4:18412039-18412058 | MS.gene061033:intron | 40.0% | |
| AATTGATGCGTTGTTGGAAG+GGG | - | chr1.4:18412072-18412091 | MS.gene061033:intron | 40.0% | |
| AGTTAATCATACCGGGTACA+AGG | + | chr1.4:18411148-18411167 | None:intergenic | 40.0% | |
| ATGCTCACATCGCCAATATT+GGG | + | chr1.4:18412039-18412058 | None:intergenic | 40.0% | |
| CAAAGGGATTGCAAGCATTA+GGG | - | chr1.4:18410742-18410761 | MS.gene061033:intron | 40.0% | |
| CCGCCATAAAGAAAGATCAA+TGG | - | chr1.4:18412102-18412121 | MS.gene061033:CDS | 40.0% | |
| CTAGGACCAAAGATATGGAT+AGG | - | chr1.4:18412144-18412163 | MS.gene061033:CDS | 40.0% | |
| GAATTGATGCGTTGTTGGAA+GGG | - | chr1.4:18412071-18412090 | MS.gene061033:intron | 40.0% | |
| GTCACAATCTGTATGCAAGT+TGG | - | chr1.4:18411392-18411411 | MS.gene061033:intron | 40.0% | |
| GTTCTATTGTGAATGGTGGT+TGG | - | chr1.4:18410819-18410838 | MS.gene061033:intron | 40.0% | |
| TCAAAGGGATTGCAAGCATT+AGG | - | chr1.4:18410741-18410760 | MS.gene061033:intron | 40.0% | |
| TCGCCAATATTGGGAAAAAC+TGG | + | chr1.4:18412030-18412049 | None:intergenic | 40.0% | |
| TTGTGGAATTGATGCGTTGT+TGG | - | chr1.4:18412066-18412085 | MS.gene061033:intron | 40.0% | |
| ! | CTTTGAGAATTCCTTGTACC+CGG | - | chr1.4:18411134-18411153 | MS.gene061033:intron | 40.0% |
| !! | GGGTTGATGAAGATAGAACT+TGG | - | chr1.4:18410762-18410781 | MS.gene061033:intron | 40.0% |
| ATGTGAGCATGGGATCATTG+TGG | - | chr1.4:18412049-18412068 | MS.gene061033:intron | 45.0% | |
| CAATATTGGCGATGTGAGCA+TGG | - | chr1.4:18412038-18412057 | MS.gene061033:intron | 45.0% | |
| CATGCTCACATCGCCAATAT+TGG | + | chr1.4:18412040-18412059 | None:intergenic | 45.0% | |
| GCAGTCCCTATCCATATCTT+TGG | + | chr1.4:18412153-18412172 | None:intergenic | 45.0% | |
| GGAATTGATGCGTTGTTGGA+AGG | - | chr1.4:18412070-18412089 | MS.gene061033:intron | 45.0% | |
| GGCGTTCCGCAACAAATATT+GGG | + | chr1.4:18411980-18411999 | None:intergenic | 45.0% | |
| TCTTTGGTCCTAGCAGCAAT+CGG | + | chr1.4:18412137-18412156 | None:intergenic | 45.0% | |
| TGCTGCTAGGACCAAAGATA+TGG | - | chr1.4:18412139-18412158 | MS.gene061033:CDS | 45.0% | |
| TGGCGTTCCGCAACAAATAT+TGG | + | chr1.4:18411981-18412000 | None:intergenic | 45.0% | |
| TTCTATTTCCCGAGCTAGAG+AGG | - | chr1.4:18410928-18410947 | MS.gene061033:intron | 45.0% | |
| ! | TGATCTTTCTTTATGGCGGG+TGG | + | chr1.4:18412101-18412120 | None:intergenic | 45.0% |
| GGCTTGATTCCTCTCTAGCT+CGG | + | chr1.4:18410940-18410959 | None:intergenic | 50.0% | |
| ! | GCTTGATTCCTCTCTAGCTC+GGG | + | chr1.4:18410939-18410958 | None:intergenic | 50.0% |
| GATCAGAGCCGATTGCTGCT+AGG | - | chr1.4:18412126-18412145 | MS.gene061033:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 18410511 | 18412214 | 18410511 | ID=MS.gene061033 |
| chr1.4 | mRNA | 18410511 | 18412214 | 18410511 | ID=MS.gene061033.t1;Parent=MS.gene061033 |
| chr1.4 | exon | 18412098 | 18412214 | 18412098 | ID=MS.gene061033.t1.exon1;Parent=MS.gene061033.t1 |
| chr1.4 | CDS | 18412098 | 18412214 | 18412098 | ID=cds.MS.gene061033.t1;Parent=MS.gene061033.t1 |
| chr1.4 | exon | 18411751 | 18412024 | 18411751 | ID=MS.gene061033.t1.exon2;Parent=MS.gene061033.t1 |
| chr1.4 | CDS | 18411751 | 18412024 | 18411751 | ID=cds.MS.gene061033.t1;Parent=MS.gene061033.t1 |
| chr1.4 | exon | 18411570 | 18411634 | 18411570 | ID=MS.gene061033.t1.exon3;Parent=MS.gene061033.t1 |
| chr1.4 | CDS | 18411570 | 18411634 | 18411570 | ID=cds.MS.gene061033.t1;Parent=MS.gene061033.t1 |
| chr1.4 | exon | 18410511 | 18410633 | 18410511 | ID=MS.gene061033.t1.exon4;Parent=MS.gene061033.t1 |
| chr1.4 | CDS | 18410511 | 18410633 | 18410511 | ID=cds.MS.gene061033.t1;Parent=MS.gene061033.t1 |
| Gene Sequence |
| Protein sequence |
