AlfalfaGEDB | MS.gene061129

M. sativa cultivar XinJiangDaYe / MS.gene061129

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061129.t1	XP_002873702.1	47.1	70	33	1	1	70	62	127	3.70E-09	70.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061129.t1	O48686	56.6	53	23	0	18	70	32	84	3.8e-10	65.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061129.t1	A0A371FQ70	63.5	52	19	0	18	69	61	112	2.7e-09	70.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene061129.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061129.t1	MTR_1g109550	48.387	62	28	1	11	68	22	83	7.04e-15	64.7
MS.gene061129.t1	MTR_5g036670	57.407	54	23	0	18	71	61	114	3.32e-14	65.9
MS.gene061129.t1	MTR_1g105960	58.000	50	21	0	18	67	19	68	3.86e-14	62.0
MS.gene061129.t1	MTR_8g062700	59.259	54	22	0	18	71	60	113	5.00e-14	65.1
MS.gene061129.t1	MTR_8g062700	59.259	54	22	0	18	71	60	113	5.09e-14	65.1
MS.gene061129.t1	MTR_1g105955	56.000	50	22	0	18	67	113	162	6.76e-14	62.8
MS.gene061129.t1	MTR_1g105955	46.667	60	28	1	4	63	24	79	2.69e-12	58.9
MS.gene061129.t1	MTR_1g093310	47.436	78	25	3	2	69	1	72	8.53e-14	60.5
MS.gene061129.t1	MTR_6g008970	56.604	53	23	0	18	70	81	133	1.13e-13	64.3
MS.gene061129.t1	MTR_3g006065	60.417	48	19	0	18	65	109	156	2.73e-13	61.6
MS.gene061129.t1	MTR_3g006185	58.000	50	21	0	18	67	117	166	8.85e-13	60.1
MS.gene061129.t1	MTR_3g006150	60.417	48	19	0	18	65	109	156	8.97e-13	59.7
MS.gene061129.t1	MTR_3g006125	44.615	65	33	1	3	67	187	248	1.72e-11	57.4
MS.gene061129.t1	MTR_6g008995	53.061	49	23	0	22	70	2	50	2.57e-11	57.4
MS.gene061129.t1	MTR_6g008995	50.943	53	26	0	18	70	55	107	3.92e-11	57.0
MS.gene061129.t1	MTR_4g133300	51.613	62	25	1	13	69	180	241	4.05e-11	56.6
MS.gene061129.t1	MTR_4g133470	51.613	62	25	1	13	69	180	241	4.96e-11	56.2
MS.gene061129.t1	MTR_3g006105	50.000	52	26	0	18	69	27	78	8.75e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061129.t1	AT1G24190	56.604	53	23	18	70	32	84	1.52e-13	63.9
MS.gene061129.t1	AT1G24190	56.604	53	23	18	70	32	84	1.62e-13	63.9
MS.gene061129.t1	AT1G24190	56.604	53	23	18	70	32	84	1.74e-13	63.5
MS.gene061129.t1	AT3G01320	57.692	52	22	18	69	75	126	6.06e-13	62.0
MS.gene061129.t1	AT3G01320	57.692	52	22	18	69	75	126	6.07e-13	62.0
MS.gene061129.t1	AT1G70060	57.692	52	22	18	69	32	83	1.08e-12	61.6
MS.gene061129.t1	AT1G70060	57.692	52	22	18	69	32	83	1.08e-12	61.6
MS.gene061129.t1	AT5G15020	52.830	53	25	18	70	70	122	4.90e-12	59.7
MS.gene061129.t1	AT5G15020	52.830	53	25	18	70	70	122	4.95e-12	59.7
MS.gene061129.t1	AT1G59890	54.717	53	24	18	70	63	115	4.99e-12	59.7
MS.gene061129.t1	AT1G59890	54.717	53	24	18	70	63	115	4.99e-12	59.7
MS.gene061129.t1	AT1G59890	54.717	53	24	18	70	63	115	5.03e-12	59.7
MS.gene061129.t1	AT1G59890	54.717	53	24	18	70	63	115	5.04e-12	59.7
MS.gene061129.t1	AT4G12020	51.923	52	25	18	69	325	376	9.33e-12	58.9
MS.gene061129.t1	AT4G12020	51.923	52	25	18	69	325	376	9.39e-12	58.9
MS.gene061129.t1	AT4G12020	51.923	52	25	18	69	325	376	9.39e-12	58.9
MS.gene061129.t1	AT4G12020	51.923	52	25	18	69	258	309	9.39e-12	58.9
MS.gene061129.t1	AT4G12020	51.923	52	25	18	69	325	376	9.39e-12	58.9
MS.gene061129.t1	AT4G12020	51.923	52	25	18	69	325	376	9.39e-12	58.9
MS.gene061129.t1	AT4G12020	51.923	52	25	18	69	325	376	9.57e-12	58.9
MS.gene061129.t1	AT4G12020	51.923	52	25	18	69	325	376	9.58e-12	58.9
MS.gene061129.t1	AT4G12020	51.923	52	25	18	69	325	376	9.96e-12	58.5
MS.gene061129.t1	AT4G12020	51.923	52	25	18	69	325	376	1.07e-11	58.5
MS.gene061129.t1	AT4G12020	51.923	52	25	18	69	325	376	1.07e-11	58.5

Find 20 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTACCTCGTCATGGTAGTTC+TGG	0.247589	1.4:+77586732	None:intergenic
CACCTCTTGTATAAATTCTT+TGG	0.253899	1.4:+77586654	None:intergenic
GGGCATACTCATTTAACTTT+GGG	0.329582	1.4:-77586602	MS.gene061129:CDS
AGTTTGATTTGATGTTCCTT+TGG	0.399942	1.4:+77586563	None:intergenic
AGGGCATACTCATTTAACTT+TGG	0.412341	1.4:-77586603	MS.gene061129:CDS
TACCTCGTCATGGTAGTTCT+GGG	0.420987	1.4:+77586733	None:intergenic
AAAAGTCCTAAAAGATATCA+AGG	0.441741	1.4:-77586678	MS.gene061129:CDS
TGATTTGATGTTCCTTTGGC+AGG	0.443659	1.4:+77586567	None:intergenic
AGAGTCAAGGAGCTGCTTGA+AGG	0.509677	1.4:-77586623	MS.gene061129:CDS
GAGTCAAGGAGCTGCTTGAA+GGG	0.541631	1.4:-77586622	MS.gene061129:CDS
TTGATGTTCCTTTGGCAGGA+AGG	0.546048	1.4:+77586571	None:intergenic
AGGTGTTGCGGCAAGAGTCA+AGG	0.556345	1.4:-77586636	MS.gene061129:CDS
CAAATCAAACTTGAGACCAC+TGG	0.570634	1.4:-77586551	MS.gene061129:CDS
ACCTCGTCATGGTAGTTCTG+GGG	0.577587	1.4:+77586734	None:intergenic
AATTTATACAAGAGGTGTTG+CGG	0.578757	1.4:-77586648	MS.gene061129:CDS
GACCAAAGAATTTATACAAG+AGG	0.598738	1.4:-77586656	MS.gene061129:CDS
CCTCGTCATGGTAGTTCTGG+GGG	0.617059	1.4:+77586735	None:intergenic
ATTCAACACCTTCCTGCCAA+AGG	0.618562	1.4:-77586579	MS.gene061129:CDS
AATGATAAATTACTAACCAG+TGG	0.644366	1.4:+77586535	None:intergenic
CAGCTTGTACTACCTCGTCA+TGG	0.666702	1.4:+77586723	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAAGTCCTAAAAGATATCA+AGG	-	chr1.4:77586714-77586733	MS.gene061129:CDS	25.0%
	CACCTCTTGTATAAATTCTT+TGG	+	chr1.4:77586741-77586760	None:intergenic	30.0%
	GACCAAAGAATTTATACAAG+AGG	-	chr1.4:77586736-77586755	MS.gene061129:CDS	30.0%
!	TTTGGTCCTTGATATCTTTT+AGG	+	chr1.4:77586723-77586742	None:intergenic	30.0%
!!	AATTTATACAAGAGGTGTTG+CGG	-	chr1.4:77586744-77586763	MS.gene061129:CDS	30.0%
!!	AGTTTGATTTGATGTTCCTT+TGG	+	chr1.4:77586832-77586851	None:intergenic	30.0%
	AATGATTCCAGTTAAAGACG+AGG	+	chr1.4:77586560-77586579	None:intergenic	35.0%
!	AGGGCATACTCATTTAACTT+TGG	-	chr1.4:77586789-77586808	MS.gene061129:intron	35.0%
!	GGGCATACTCATTTAACTTT+GGG	-	chr1.4:77586790-77586809	MS.gene061129:intron	35.0%
	CAAATCAAACTTGAGACCAC+TGG	-	chr1.4:77586841-77586860	MS.gene061129:intron	40.0%
	TGATTTGATGTTCCTTTGGC+AGG	+	chr1.4:77586828-77586847	None:intergenic	40.0%
	ATTCAACACCTTCCTGCCAA+AGG	-	chr1.4:77586813-77586832	MS.gene061129:intron	45.0%
	GTATGCTCCTCGTCTTTAAC+TGG	-	chr1.4:77586550-77586569	MS.gene061129:CDS	45.0%
	TACCTCGTCATGGTAGTTCT+GGG	+	chr1.4:77586662-77586681	None:intergenic	45.0%
	TTGATGTTCCTTTGGCAGGA+AGG	+	chr1.4:77586824-77586843	None:intergenic	45.0%
	AGAGTCAAGGAGCTGCTTGA+AGG	-	chr1.4:77586769-77586788	MS.gene061129:intron	50.0%
	CAGCTTGTACTACCTCGTCA+TGG	+	chr1.4:77586672-77586691	None:intergenic	50.0%
	CTACCTCGTCATGGTAGTTC+TGG	+	chr1.4:77586663-77586682	None:intergenic	50.0%
	GAGTCAAGGAGCTGCTTGAA+GGG	-	chr1.4:77586770-77586789	MS.gene061129:intron	50.0%
!	ACCTCGTCATGGTAGTTCTG+GGG	+	chr1.4:77586661-77586680	None:intergenic	50.0%
!	AGGTGTTGCGGCAAGAGTCA+AGG	-	chr1.4:77586756-77586775	MS.gene061129:intron	55.0%
!!	CCTCGTCATGGTAGTTCTGG+GGG	+	chr1.4:77586660-77586679	None:intergenic	55.0%
	CCCCCAGAACTACCATGACG+AGG	-	chr1.4:77586657-77586676	MS.gene061129:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	77586547	77586867	77586547	ID=MS.gene061129
chr1.4	mRNA	77586547	77586867	77586547	ID=MS.gene061129.t1;Parent=MS.gene061129
chr1.4	exon	77586865	77586867	77586865	ID=MS.gene061129.t1.exon1;Parent=MS.gene061129.t1
chr1.4	CDS	77586865	77586867	77586865	ID=cds.MS.gene061129.t1;Parent=MS.gene061129.t1
chr1.4	exon	77586547	77586765	77586547	ID=MS.gene061129.t1.exon2;Parent=MS.gene061129.t1
chr1.4	CDS	77586547	77586765	77586547	ID=cds.MS.gene061129.t1;Parent=MS.gene061129.t1

Gene Sequence	>MS.gene061129 ATGGTATGCTCCTCGTCTTTAACTGGAATCATTGATTTTTTTGTTTTATTATTGTGAATTATTGAAATTAGTTATACTGAATCATTACTTTTCATAATAAAGATGAAGATCCCCCAGAACTACCATGACGAGGTAGTACAAGCTGCGTTTAAACATAATGAGCTTTTAAAAGTCCTAAAAGATATCAAGGACCAAAGAATTTATACAAGAGGTGTTGCGGCAAGAGTCAAGGAGCTGCTTGAAGGGCATACTCATTTAACTTTGGGATTCAACACCTTCCTGCCAAAGGAACATCAAATCAAACTTGAGACCACTGGTTAG
Protein sequence	>MS.gene061129.t1 MMKIPQNYHDEVVQAAFKHNELLKVLKDIKDQRIYTRGVAARVKELLEGHTHLTLGFNTFLPKEHQIKLETTG