AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061156

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061156.t1 MTR_4g035820 48.867 309 138 2 17 325 144 432 2.22e-91 293
MS.gene061156.t1 MTR_5g017720 79.042 167 10 1 327 493 18 159 6.73e-87 265
MS.gene061156.t1 MTR_6g014355 76.230 122 29 0 16 137 9 130 1.06e-56 189
MS.gene061156.t1 MTR_6g014355 69.565 138 36 2 240 377 114 245 1.08e-56 189
MS.gene061156.t1 MTR_3g450850 38.182 165 100 1 17 181 124 286 5.81e-30 120
MS.gene061156.t1 MTR_8g447350 43.284 134 49 4 279 407 1 112 4.22e-26 105
MS.gene061156.t1 MTR_2g035140 36.364 176 87 5 309 475 104 263 7.66e-24 101
MS.gene061156.t1 MTR_2g035140 44.348 115 47 4 78 192 13 110 2.57e-18 85.5
MS.gene061156.t1 MTR_3g012160 44.086 93 52 0 90 182 1 93 1.69e-20 86.7
MS.gene061156.t1 MTR_6g051425 29.944 177 111 4 17 189 91 258 1.08e-17 84.7
MS.gene061156.t1 MTR_1g070355 28.743 167 113 3 17 183 145 305 9.73e-14 73.9
MS.gene061156.t1 MTR_8g446020 26.163 172 119 4 18 187 85 250 1.47e-12 68.2
MS.gene061156.t1 MTR_0558s0020 25.581 172 120 4 18 187 125 290 6.36e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061156.t1 AT5G28530 28.440 436 239 9 17 390 125 549 1.94e-52 187
MS.gene061156.t1 AT5G28530 28.211 436 244 9 17 390 125 553 1.99e-52 187
MS.gene061156.t1 AT1G10240 36.957 230 134 4 169 392 380 604 4.87e-41 157
MS.gene061156.t1 AT1G10240 39.306 173 103 1 17 189 120 290 5.14e-36 142
MS.gene061156.t1 AT1G10240 36.957 230 134 4 169 392 380 604 4.87e-41 157
MS.gene061156.t1 AT1G10240 39.306 173 103 1 17 189 120 290 5.14e-36 142
MS.gene061156.t1 AT5G28530 31.839 223 141 4 170 390 399 612 4.04e-32 130
MS.gene061156.t1 AT5G28530 33.708 178 106 2 17 184 125 300 1.20e-24 108
MS.gene061156.t1 AT5G28530 31.839 223 141 4 170 390 399 612 4.04e-32 130
MS.gene061156.t1 AT5G28530 33.708 178 106 2 17 184 125 300 1.20e-24 108
MS.gene061156.t1 AT5G28530 31.390 223 146 4 170 390 399 616 4.33e-32 130
MS.gene061156.t1 AT5G28530 33.708 178 106 2 17 184 125 300 1.07e-24 108
MS.gene061156.t1 AT4G38170 30.859 256 162 7 151 400 158 404 1.59e-30 125
MS.gene061156.t1 AT4G38180 30.047 213 144 1 186 393 420 632 9.38e-28 118
MS.gene061156.t1 AT4G38180 29.480 173 110 3 15 184 144 307 8.70e-20 93.6
MS.gene061156.t1 AT4G19990 27.839 273 168 7 152 399 238 506 1.41e-24 108
MS.gene061156.t1 AT4G19990 27.839 273 168 7 152 399 295 563 1.43e-24 108
MS.gene061156.t1 AT4G19990 27.839 273 168 7 152 399 309 577 1.63e-24 108
MS.gene061156.t1 AT4G19990 27.839 273 168 7 152 399 295 563 1.71e-24 107
MS.gene061156.t1 AT4G19990 27.839 273 168 7 152 399 295 563 1.71e-24 107
MS.gene061156.t1 AT4G19990 27.839 273 168 7 152 399 295 563 1.79e-24 107
MS.gene061156.t1 AT4G19990 27.839 273 168 7 152 399 295 563 1.79e-24 107
MS.gene061156.t1 AT3G22170 28.182 220 152 3 183 397 402 620 3.72e-24 107
MS.gene061156.t1 AT3G22170 28.182 220 152 3 183 397 402 620 3.72e-24 107
MS.gene061156.t1 AT1G76320 27.315 216 151 3 179 390 326 539 9.39e-24 105
MS.gene061156.t1 AT1G76320 30.811 185 111 5 2 183 49 219 8.13e-15 77.8
MS.gene061156.t1 AT1G76320 27.315 216 151 3 179 390 326 539 1.01e-23 105
MS.gene061156.t1 AT1G76320 30.811 185 111 5 2 183 49 219 6.43e-15 78.2
MS.gene061156.t1 AT1G76320 27.315 216 151 3 179 390 326 539 1.01e-23 105
MS.gene061156.t1 AT1G76320 30.811 185 111 5 2 183 49 219 6.43e-15 78.2
MS.gene061156.t1 AT1G76320 27.315 216 151 3 179 390 326 539 1.07e-23 105
MS.gene061156.t1 AT1G76320 30.811 185 111 5 2 183 49 219 6.47e-15 78.2
MS.gene061156.t1 AT4G15090 28.230 209 146 2 189 393 399 607 5.15e-23 103
MS.gene061156.t1 AT4G15090 27.647 170 116 3 15 183 119 282 8.24e-13 71.6
MS.gene061156.t1 AT4G15090 28.230 209 146 2 189 393 399 607 5.15e-23 103
MS.gene061156.t1 AT4G15090 27.647 170 116 3 15 183 119 282 8.24e-13 71.6
MS.gene061156.t1 AT4G15090 28.230 209 146 2 189 393 399 607 5.15e-23 103
MS.gene061156.t1 AT4G15090 27.647 170 116 3 15 183 119 282 8.24e-13 71.6
MS.gene061156.t1 AT4G15090 28.230 209 146 2 189 393 399 607 5.15e-23 103
MS.gene061156.t1 AT4G15090 27.647 170 116 3 15 183 119 282 8.24e-13 71.6
MS.gene061156.t1 AT4G15090 28.230 209 146 2 189 393 399 607 5.15e-23 103
MS.gene061156.t1 AT4G15090 27.647 170 116 3 15 183 119 282 8.24e-13 71.6
MS.gene061156.t1 AT2G27110 27.273 231 158 5 186 410 222 448 1.42e-21 99.0
MS.gene061156.t1 AT2G27110 27.706 231 157 5 186 410 367 593 1.64e-21 99.0
MS.gene061156.t1 AT2G27110 27.706 231 157 5 186 410 367 593 1.64e-21 99.0
MS.gene061156.t1 AT3G06250 28.384 229 153 4 183 403 490 715 8.03e-21 96.7
MS.gene061156.t1 AT3G06250 28.384 229 153 4 183 403 490 715 8.03e-21 96.7
MS.gene061156.t1 AT3G06250 28.384 229 153 4 183 403 490 715 8.03e-21 96.7
MS.gene061156.t1 AT5G18960 26.437 261 171 7 152 403 491 739 4.71e-20 94.4
MS.gene061156.t1 AT5G18960 26.437 261 171 7 152 403 491 739 4.71e-20 94.4
MS.gene061156.t1 AT1G52520 27.434 226 151 4 181 393 411 636 1.41e-19 92.8
MS.gene061156.t1 AT2G32250 25.728 206 149 2 191 392 345 550 7.01e-17 84.3
MS.gene061156.t1 AT2G32250 25.728 206 149 2 191 392 345 550 7.93e-17 84.3
MS.gene061156.t1 AT2G32250 25.728 206 149 2 191 392 345 550 7.93e-17 84.3
MS.gene061156.t1 AT2G32250 25.728 206 149 2 191 392 345 550 8.83e-17 84.0
MS.gene061156.t1 AT2G32250 25.728 206 149 2 191 392 345 550 8.83e-17 84.0
MS.gene061156.t1 AT2G32250 25.728 206 149 2 191 392 345 550 8.83e-17 84.0
MS.gene061156.t1 AT2G32250 25.728 206 149 2 191 392 304 509 9.32e-17 84.0
MS.gene061156.t1 AT2G32250 25.728 206 149 2 191 392 304 509 9.98e-17 84.0
MS.gene061156.t1 AT1G80010 22.174 230 156 5 181 393 436 659 5.35e-11 65.9

Find 81 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTTCGTGGACATTTCTT+TGG 0.169134 1.4:-77884942 MS.gene061156:CDS
CACAAAATCATAAAGTCTTA+TGG 0.192084 1.4:-77884264 MS.gene061156:CDS
TCAATAACATGCCATTCTTT+TGG 0.225382 1.4:+77885937 None:intergenic
ATCTTCGTGGACATTTCTTT+GGG 0.252767 1.4:-77884941 MS.gene061156:CDS
CTTTGAACTTGCTCCTCTAT+AGG 0.259030 1.4:+77884406 None:intergenic
TAAGATTCACCAAGGTAAAT+TGG 0.262024 1.4:-77885698 MS.gene061156:CDS
TAAGACTTTAAATATAAAAT+AGG 0.262718 1.4:+77883794 None:intergenic
TTCTCCACATTCTCAATATA+TGG 0.265541 1.4:+77884040 None:intergenic
TTTGAACTTGCTCCTCTATA+GGG 0.267606 1.4:+77884407 None:intergenic
CACCATGTTGGATACATATA+GGG 0.299499 1.4:-77884452 MS.gene061156:CDS
TATCAACAGTGAATGCAAAT+TGG 0.310367 1.4:+77885614 None:intergenic
ATTATAAGAATTCACCTTGT+AGG 0.317528 1.4:+77885501 None:intergenic
AAAGACTAGAGAATATATTT+TGG 0.320756 1.4:-77885585 MS.gene061156:CDS
GAAGCAAACAAACACATAAT+AGG 0.338772 1.4:-77884999 MS.gene061156:CDS
TTTGAGTTGTACCAAGAGTA+TGG 0.355497 1.4:-77885547 MS.gene061156:CDS
TGAATATCTTTCCGAAGAAA+TGG 0.356103 1.4:+77885733 None:intergenic
ACACCATGTTGGATACATAT+AGG 0.358167 1.4:-77884453 MS.gene061156:CDS
ACAAAATCATAAAGTCTTAT+GGG 0.374722 1.4:-77884263 MS.gene061156:CDS
ATCTTGCCATTGAGGACATT+GGG 0.386566 1.4:-77884492 MS.gene061156:CDS
CAAGCAAATAATGCGTGTTA+TGG 0.401498 1.4:-77885788 MS.gene061156:CDS
CTTAGTGAAGTAGGACATAA+AGG 0.408462 1.4:-77884070 MS.gene061156:CDS
ATGGTGATCTTCCATTTCTT+CGG 0.414681 1.4:-77885744 MS.gene061156:CDS
GGAAGATCAGAGAGTATAAA+CGG 0.414813 1.4:-77884903 MS.gene061156:CDS
GTTTGCTTCTAGATTGTACT+TGG 0.419002 1.4:+77885013 None:intergenic
TTGCCCTCTCAAACTCCTCC+TGG 0.438199 1.4:+77884353 None:intergenic
TTATGGGATGGTGATGTTGT+TGG 0.458032 1.4:-77884247 MS.gene061156:CDS
GCAAGGAAGCACATTTGATC+TGG 0.458873 1.4:+77885880 None:intergenic
GAAACATAACCTTCTTTGTC+CGG 0.471805 1.4:+77883872 None:intergenic
TGGGTACCGGCTTATCTTCG+TGG 0.475874 1.4:-77884954 MS.gene061156:CDS
GTATGAACTTAAGCAACATT+GGG 0.480454 1.4:-77884973 MS.gene061156:CDS
AGCATGACATTTACTTCGAC+TGG 0.480943 1.4:+77884091 None:intergenic
TCAAGGTGTGGATGCAAAGC+GGG 0.486956 1.4:-77885997 MS.gene061156:intron
TGTATGAACTTAAGCAACAT+TGG 0.490968 1.4:-77884974 MS.gene061156:CDS
TTAGGTTGATCTTGCCATTG+AGG 0.494408 1.4:-77884500 MS.gene061156:intron
CCCAAAACAAAACGATCAAA+AGG 0.499343 1.4:-77883968 MS.gene061156:CDS
GAGGTAGTAAATCATGATTA+TGG 0.500482 1.4:+77885902 None:intergenic
AATGTCATGCTTAGTGAAGT+AGG 0.500833 1.4:-77884079 MS.gene061156:CDS
CAACGGCATCTCCATACTCT+TGG 0.501798 1.4:+77885536 None:intergenic
CGTAATGGCAGATACCTTGT+TGG 0.505068 1.4:+77884139 None:intergenic
TCACAATGTTACAACTTGTC+CGG 0.507013 1.4:-77883891 MS.gene061156:CDS
TGCATTTGACACTACCTACA+AGG 0.509568 1.4:-77885515 MS.gene061156:intron
CTTCGTGGACATTTCTTTGG+GGG 0.512433 1.4:-77884939 MS.gene061156:CDS
TCGATCATTATCCCCTATAG+AGG 0.513231 1.4:-77884419 MS.gene061156:CDS
GATCTTGCCATTGAGGACAT+TGG 0.526255 1.4:-77884493 MS.gene061156:CDS
CCTCCGCGTTCAATAACAAA+AGG 0.532998 1.4:-77883995 MS.gene061156:CDS
TTGTTGCCCAATGTCCTCAA+TGG 0.536367 1.4:+77884486 None:intergenic
ACAACTTGTCCGGACAAAGA+AGG 0.546455 1.4:-77883881 MS.gene061156:CDS
TCTTCGTGGACATTTCTTTG+GGG 0.547100 1.4:-77884940 MS.gene061156:CDS
TCATCACGACACCAACGTAA+TGG 0.552128 1.4:+77884124 None:intergenic
ATTTACTGTGAAATATTACA+AGG 0.556334 1.4:-77884296 MS.gene061156:CDS
TTGAACTTGCTCCTCTATAG+GGG 0.562876 1.4:+77884408 None:intergenic
TAGTAAATCATGATTATGGT+TGG 0.565674 1.4:+77885906 None:intergenic
GAAGATCAGAGAGTATAAAC+GGG 0.569571 1.4:-77884902 MS.gene061156:CDS
CATCACCATCCAATTTACCT+TGG 0.575070 1.4:+77885689 None:intergenic
GGGTCCATATATTGAGAATG+TGG 0.577307 1.4:-77884044 MS.gene061156:CDS
CTCAATCAATTGTTCTCATG+TGG 0.580264 1.4:-77886586 MS.gene061156:CDS
AAATGTCCACGAAGATAAGC+CGG 0.581287 1.4:+77884948 None:intergenic
CATCTTCCTCAGAAATGTTG+CGG 0.581639 1.4:+77885851 None:intergenic
AATCATAAAGTCTTATGGGA+TGG 0.585524 1.4:-77884259 MS.gene061156:CDS
ACTTAAGCAACATTGGGTAC+CGG 0.589921 1.4:-77884967 MS.gene061156:CDS
GAAGTAGGACATAAAGGCAA+GGG 0.591654 1.4:-77884064 MS.gene061156:CDS
TTCAAGGTGTGGATGCAAAG+CGG 0.592179 1.4:-77885998 MS.gene061156:intron
ATTCACCAAGGTAAATTGGA+TGG 0.593411 1.4:-77885694 MS.gene061156:CDS
AGTTCCAGGAGGAGTTTGAG+AGG 0.593892 1.4:-77884357 MS.gene061156:CDS
ACAACTACATCACACCATGT+TGG 0.599668 1.4:-77884464 MS.gene061156:CDS
AAATGTTGCGGTAAGATGCA+AGG 0.600031 1.4:+77885863 None:intergenic
GTTCCAGGAGGAGTTTGAGA+GGG 0.605391 1.4:-77884356 MS.gene061156:CDS
TGAAGTAGGACATAAAGGCA+AGG 0.605684 1.4:-77884065 MS.gene061156:CDS
AGGAAGCACATTTGATCTGG+AGG 0.612453 1.4:+77885883 None:intergenic
AAGGTGGAATCGAATTGTCA+AGG 0.613048 1.4:-77883949 MS.gene061156:CDS
GTAGGTAGTGTCAAATGCAA+CGG 0.614333 1.4:+77885519 None:intergenic
ACCATGTTGGATACATATAG+GGG 0.617078 1.4:-77884451 MS.gene061156:CDS
TACAACTCAAAGCAATGAGG+TGG 0.628223 1.4:+77885559 None:intergenic
AAAACAAAACGATCAAAAGG+TGG 0.630073 1.4:-77883965 MS.gene061156:CDS
ATCTTACCGCAACATTTCTG+AGG 0.633790 1.4:-77885857 MS.gene061156:CDS
TTCTTCAATAAGATTCACCA+AGG 0.638648 1.4:-77885706 MS.gene061156:CDS
ATTGCTTTCAAATTCCAACA+AGG 0.667427 1.4:-77884153 MS.gene061156:CDS
TCCCCTATATGTATCCAACA+TGG 0.671193 1.4:+77884450 None:intergenic
CAAGGTATCTGCCATTACGT+TGG 0.682443 1.4:-77884135 MS.gene061156:CDS
TGGTACAACTCAAAGCAATG+AGG 0.685743 1.4:+77885556 None:intergenic
TGCGTTTAAGAAGTTCCAGG+AGG 0.692916 1.4:-77884368 MS.gene061156:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGACTTTAAATATAAAAT+AGG + chr1.4:77886605-77886624 None:intergenic 10.0%
!!! TTTTTTTTTTCTATTAACTT+TGG - chr1.4:77885043-77885062 MS.gene061156:CDS 10.0%
!! AAAAACAAATAGAAAATACA+TGG + chr1.4:77885103-77885122 None:intergenic 15.0%
!! AAAACAAATAGAAAATACAT+GGG + chr1.4:77885102-77885121 None:intergenic 15.0%
!! AATGATACTTTAATAACTAA+TGG - chr1.4:77885847-77885866 MS.gene061156:CDS 15.0%
!! CGATTTCTTAAAAAAAATAT+GGG - chr1.4:77884283-77884302 MS.gene061156:CDS 15.0%
!! TAATATGAATTTATTTGTGT+TGG - chr1.4:77884038-77884057 MS.gene061156:CDS 15.0%
!! TCGATTTCTTAAAAAAAATA+TGG - chr1.4:77884282-77884301 MS.gene061156:CDS 15.0%
!!! AATTCAACAATAATATTTTG+TGG - chr1.4:77883972-77883991 MS.gene061156:CDS 15.0%
!! AAACAAATAGAAAATACATG+GGG + chr1.4:77885101-77885120 None:intergenic 20.0%
!! AATTCGTATATAAGGAATTA+GGG + chr1.4:77883956-77883975 None:intergenic 20.0%
!! ATTTACTGTGAAATATTACA+AGG - chr1.4:77886100-77886119 MS.gene061156:intron 20.0%
!! TATTGTTGAATTCGTATATA+AGG + chr1.4:77883964-77883983 None:intergenic 20.0%
!! TTGAGAACAATATAAGAAAA+AGG + chr1.4:77883884-77883903 None:intergenic 20.0%
!!! AAAGACTAGAGAATATATTT+TGG - chr1.4:77884811-77884830 MS.gene061156:intron 20.0%
!!! AATTCTTATAATATGCCTTT+TGG - chr1.4:77884906-77884925 MS.gene061156:CDS 20.0%
!!! AATTGTTTTGAACCTTTTAT+TGG - chr1.4:77884178-77884197 MS.gene061156:CDS 20.0%
!!! ACAAAATCATAAAGTCTTAT+GGG - chr1.4:77886133-77886152 MS.gene061156:intron 20.0%
!!! TTCGAAATCAATATTTTTCA+TGG - chr1.4:77885287-77885306 MS.gene061156:intron 20.0%
! AAAAATATGGGATGATACAT+AGG - chr1.4:77884295-77884314 MS.gene061156:CDS 25.0%
! AGAAAACATTGTCCAATAAA+AGG + chr1.4:77884193-77884212 None:intergenic 25.0%
! AGTAATATGCCATATACAAA+AGG + chr1.4:77885236-77885255 None:intergenic 25.0%
! ATTATAAGAATTCACCTTGT+AGG + chr1.4:77884898-77884917 None:intergenic 25.0%
! ATTCGTATATAAGGAATTAG+GGG + chr1.4:77883955-77883974 None:intergenic 25.0%
! CAAAACATAGTATAAACACA+GGG + chr1.4:77885651-77885670 None:intergenic 25.0%
! CACAAAATCATAAAGTCTTA+TGG - chr1.4:77886132-77886151 MS.gene061156:intron 25.0%
! CATATTACTGCTAAATTTAG+TGG - chr1.4:77885247-77885266 MS.gene061156:intron 25.0%
! CTTGAGAAAAATGTTAAGTA+TGG - chr1.4:77884633-77884652 MS.gene061156:intron 25.0%
! GAATTCGTATATAAGGAATT+AGG + chr1.4:77883957-77883976 None:intergenic 25.0%
! TAGTAAATCATGATTATGGT+TGG + chr1.4:77884493-77884512 None:intergenic 25.0%
! TTAATGAGAATTATGCCAAA+AGG - chr1.4:77884236-77884255 MS.gene061156:CDS 25.0%
! TTATCACATGGTTCATTATT+TGG + chr1.4:77884100-77884119 None:intergenic 25.0%
!! TCATTTTCTGAATTATCACA+TGG + chr1.4:77884112-77884131 None:intergenic 25.0%
!! TTGAGATTTTTCCAAAAGAA+TGG - chr1.4:77884448-77884467 MS.gene061156:CDS 25.0%
!!! GGATCATGATATTTTTTTTC+TGG + chr1.4:77884351-77884370 None:intergenic 25.0%
!!! TGAATTTATTTGTGTTGGTT+AGG - chr1.4:77884043-77884062 MS.gene061156:CDS 25.0%
!!! TTCTTTTGGAAAAATCTCAA+AGG + chr1.4:77884448-77884467 None:intergenic 25.0%
!!! TTGTAAGCATTTTGAGTTTT+GGG - chr1.4:77886178-77886197 MS.gene061156:intron 25.0%
AAAACAAAACGATCAAAAGG+TGG - chr1.4:77886431-77886450 MS.gene061156:intron 30.0%
AAACTTTGTCTACATTTCGA+TGG - chr1.4:77884994-77885013 MS.gene061156:CDS 30.0%
AACGAAATAGACAATCTTCA+AGG - chr1.4:77884382-77884401 MS.gene061156:CDS 30.0%
ATTCCAACAAAAATGCCAAA+AGG + chr1.4:77884924-77884943 None:intergenic 30.0%
ATTGCTTTCAAATTCCAACA+AGG - chr1.4:77886243-77886262 MS.gene061156:intron 30.0%
CCAAAACATAGTATAAACAC+AGG + chr1.4:77885652-77885671 None:intergenic 30.0%
CTATTTCGTTGTTCTTTAGA+TGG + chr1.4:77884372-77884391 None:intergenic 30.0%
GAAGCAAACAAACACATAAT+AGG - chr1.4:77885397-77885416 MS.gene061156:intron 30.0%
GAGGTAGTAAATCATGATTA+TGG + chr1.4:77884497-77884516 None:intergenic 30.0%
GTATGAACTTAAGCAACATT+GGG - chr1.4:77885423-77885442 MS.gene061156:intron 30.0%
TAAGATTCACCAAGGTAAAT+TGG - chr1.4:77884698-77884717 MS.gene061156:intron 30.0%
TATCAACAGTGAATGCAAAT+TGG + chr1.4:77884785-77884804 None:intergenic 30.0%
TGAATATCTTTCCGAAGAAA+TGG + chr1.4:77884666-77884685 None:intergenic 30.0%
TGTATGAACTTAAGCAACAT+TGG - chr1.4:77885422-77885441 MS.gene061156:intron 30.0%
TTACTGCTAAATTTAGTGGT+TGG - chr1.4:77885251-77885270 MS.gene061156:intron 30.0%
TTCTCCACATTCTCAATATA+TGG + chr1.4:77886359-77886378 None:intergenic 30.0%
TTCTTCAATAAGATTCACCA+AGG - chr1.4:77884690-77884709 MS.gene061156:intron 30.0%
! AATCATAAAGTCTTATGGGA+TGG - chr1.4:77886137-77886156 MS.gene061156:intron 30.0%
! CAAAACATGCCTTTTGTATA+TGG - chr1.4:77885224-77885243 MS.gene061156:intron 30.0%
! CCTGTGTTTATACTATGTTT+TGG - chr1.4:77885649-77885668 MS.gene061156:CDS 30.0%
! TCAATAACATGCCATTCTTT+TGG + chr1.4:77884462-77884481 None:intergenic 30.0%
! TTCATTCTTCCTCTTTTTGA+TGG + chr1.4:77886558-77886577 None:intergenic 30.0%
!! GTGTTTATACTATGTTTTGG+AGG - chr1.4:77885652-77885671 MS.gene061156:CDS 30.0%
!! TATACAAAAGGCATGTTTTG+TGG + chr1.4:77885224-77885243 None:intergenic 30.0%
!! TTGACGATTTTGAAGAAGAA+TGG - chr1.4:77885347-77885366 MS.gene061156:intron 30.0%
!! TTTTAAGAAATCGACCTTTC+CGG + chr1.4:77884276-77884295 None:intergenic 30.0%
!!! ACCTTTTGATCGTTTTGTTT+TGG + chr1.4:77886432-77886451 None:intergenic 30.0%
!!! CCTTTTGATCGTTTTGTTTT+GGG + chr1.4:77886431-77886450 None:intergenic 30.0%
!!! GTTGTAAGCATTTTGAGTTT+TGG - chr1.4:77886177-77886196 MS.gene061156:intron 30.0%
!!! TATGTTTTGGAGGATAGTTT+TGG - chr1.4:77885662-77885681 MS.gene061156:CDS 30.0%
!!! TGTAAGCATTTTGAGTTTTG+GGG - chr1.4:77886179-77886198 MS.gene061156:intron 30.0%
!!! TTTTTTCTGGAAATCCTTCT+CGG + chr1.4:77884338-77884357 None:intergenic 30.0%
AACATAGTATAAACACAGGG+AGG + chr1.4:77885648-77885667 None:intergenic 35.0%
AATAGACAATCTTCAAGGTG+TGG - chr1.4:77884387-77884406 MS.gene061156:CDS 35.0%
AATATGGGATGATACATAGG+AGG - chr1.4:77884298-77884317 MS.gene061156:CDS 35.0%
AATGTCATGCTTAGTGAAGT+AGG - chr1.4:77886317-77886336 MS.gene061156:intron 35.0%
ACACCATGTTGGATACATAT+AGG - chr1.4:77885943-77885962 MS.gene061156:CDS 35.0%
ACCATGTTGGATACATATAG+GGG - chr1.4:77885945-77885964 MS.gene061156:CDS 35.0%
AGAATTATGCCAAAAGGAGT+GGG - chr1.4:77884242-77884261 MS.gene061156:CDS 35.0%
ATATGGGATGATACATAGGA+GGG - chr1.4:77884299-77884318 MS.gene061156:CDS 35.0%
ATCTTCGTGGACATTTCTTT+GGG - chr1.4:77885455-77885474 MS.gene061156:intron 35.0%
ATGGTGATCTTCCATTTCTT+CGG - chr1.4:77884652-77884671 MS.gene061156:intron 35.0%
ATTCACCAAGGTAAATTGGA+TGG - chr1.4:77884702-77884721 MS.gene061156:intron 35.0%
CAAGCAAATAATGCGTGTTA+TGG - chr1.4:77884608-77884627 MS.gene061156:intron 35.0%
CACCATGTTGGATACATATA+GGG - chr1.4:77885944-77885963 MS.gene061156:CDS 35.0%
CCAAGGTGAAAACATTAACA+AGG + chr1.4:77885606-77885625 None:intergenic 35.0%
CCCAAAACAAAACGATCAAA+AGG - chr1.4:77886428-77886447 MS.gene061156:intron 35.0%
CTCAATCAATTGTTCTCATG+TGG - chr1.4:77883810-77883829 MS.gene061156:CDS 35.0%
CTTAGTGAAGTAGGACATAA+AGG - chr1.4:77886326-77886345 MS.gene061156:intron 35.0%
GAAACATAACCTTCTTTGTC+CGG + chr1.4:77886527-77886546 None:intergenic 35.0%
GTTTGCTTCTAGATTGTACT+TGG + chr1.4:77885386-77885405 None:intergenic 35.0%
TATCTTCGTGGACATTTCTT+TGG - chr1.4:77885454-77885473 MS.gene061156:intron 35.0%
TCACAATGTTACAACTTGTC+CGG - chr1.4:77886505-77886524 MS.gene061156:intron 35.0%
TGATCGGTAATGATTGTCTT+TGG + chr1.4:77885160-77885179 None:intergenic 35.0%
TTTACTCTACTCAAACACCA+AGG + chr1.4:77885623-77885642 None:intergenic 35.0%
TTTGTTGGAATCGACAATCA+CGG - chr1.4:77884933-77884952 MS.gene061156:CDS 35.0%
! ACAATTTTGTAAGTGTGCCA+AGG - chr1.4:77884743-77884762 MS.gene061156:intron 35.0%
! ATGCCTTTTGGCATTTTTGT+TGG - chr1.4:77884918-77884937 MS.gene061156:CDS 35.0%
! CCTTGTTAATGTTTTCACCT+TGG - chr1.4:77885603-77885622 MS.gene061156:CDS 35.0%
! GAAGATCAGAGAGTATAAAC+GGG - chr1.4:77885494-77885513 MS.gene061156:intron 35.0%
! GGAAGATCAGAGAGTATAAA+CGG - chr1.4:77885493-77885512 MS.gene061156:intron 35.0%
! TATTTTGCTCTTCACAAGTG+TGG - chr1.4:77885751-77885770 MS.gene061156:CDS 35.0%
! TGGACAATGTTTTCTAAGTG+AGG - chr1.4:77884198-77884217 MS.gene061156:CDS 35.0%
! TGTAGCTTTTGCAATAGAAG+TGG - chr1.4:77886482-77886501 MS.gene061156:intron 35.0%
! TTTGAACTTGCTCCTCTATA+GGG + chr1.4:77885992-77886011 None:intergenic 35.0%
! TTTTGCGTTTAAGAAGTTCC+AGG - chr1.4:77886025-77886044 MS.gene061156:intron 35.0%
!! TTTGAGTTGTACCAAGAGTA+TGG - chr1.4:77884849-77884868 MS.gene061156:CDS 35.0%
AAATGTCCACGAAGATAAGC+CGG + chr1.4:77885451-77885470 None:intergenic 40.0%
AAATGTTGCGGTAAGATGCA+AGG + chr1.4:77884536-77884555 None:intergenic 40.0%
AAGGTGGAATCGAATTGTCA+AGG - chr1.4:77886447-77886466 MS.gene061156:intron 40.0%
ACAACTACATCACACCATGT+TGG - chr1.4:77885932-77885951 MS.gene061156:CDS 40.0%
ACGTGTAGTATGCATCTGTT+AGG - chr1.4:77885696-77885715 MS.gene061156:CDS 40.0%
ACTTAAGCAACATTGGGTAC+CGG - chr1.4:77885429-77885448 MS.gene061156:intron 40.0%
AGCATGACATTTACTTCGAC+TGG + chr1.4:77886308-77886327 None:intergenic 40.0%
AGTATGCATCTGTTAGGAGT+TGG - chr1.4:77885702-77885721 MS.gene061156:CDS 40.0%
AGTCATCCATGGATCTTGAT+CGG + chr1.4:77885176-77885195 None:intergenic 40.0%
ATCTTACCGCAACATTTCTG+AGG - chr1.4:77884539-77884558 MS.gene061156:intron 40.0%
ATCTTGCCATTGAGGACATT+GGG - chr1.4:77885904-77885923 MS.gene061156:CDS 40.0%
CACGGAAGGACAATATTGTT+TGG - chr1.4:77884951-77884970 MS.gene061156:CDS 40.0%
CATCACCATCCAATTTACCT+TGG + chr1.4:77884710-77884729 None:intergenic 40.0%
CATCTTCCTCAGAAATGTTG+CGG + chr1.4:77884548-77884567 None:intergenic 40.0%
CGTCATCAACCATCAAAAAG+AGG - chr1.4:77886546-77886565 MS.gene061156:intron 40.0%
GAAGTAGGACATAAAGGCAA+GGG - chr1.4:77886332-77886351 MS.gene061156:intron 40.0%
GAGAATTATGCCAAAAGGAG+TGG - chr1.4:77884241-77884260 MS.gene061156:CDS 40.0%
GGGTCCATATATTGAGAATG+TGG - chr1.4:77886352-77886371 MS.gene061156:intron 40.0%
GTAGGTAGTGTCAAATGCAA+CGG + chr1.4:77884880-77884899 None:intergenic 40.0%
GTGTGGTTTATGCATCTGTT+AGG - chr1.4:77885768-77885787 MS.gene061156:CDS 40.0%
TACAACTCAAAGCAATGAGG+TGG + chr1.4:77884840-77884859 None:intergenic 40.0%
TCATTACCGATCAAGATCCA+TGG - chr1.4:77885167-77885186 MS.gene061156:intron 40.0%
TCCCCTATATGTATCCAACA+TGG + chr1.4:77885949-77885968 None:intergenic 40.0%
TCGATCATTATCCCCTATAG+AGG - chr1.4:77885977-77885996 MS.gene061156:CDS 40.0%
TCTTCGTGGACATTTCTTTG+GGG - chr1.4:77885456-77885475 MS.gene061156:intron 40.0%
TGAAGTAGGACATAAAGGCA+AGG - chr1.4:77886331-77886350 MS.gene061156:intron 40.0%
TGCATTTGACACTACCTACA+AGG - chr1.4:77884881-77884900 MS.gene061156:CDS 40.0%
TGGTACAACTCAAAGCAATG+AGG + chr1.4:77884843-77884862 None:intergenic 40.0%
! CTTTGAACTTGCTCCTCTAT+AGG + chr1.4:77885993-77886012 None:intergenic 40.0%
! GAAGCAAGAGTTTTCATCCT+TGG + chr1.4:77884763-77884782 None:intergenic 40.0%
! TTATGGGATGGTGATGTTGT+TGG - chr1.4:77886149-77886168 MS.gene061156:intron 40.0%
! TTGAACTTGCTCCTCTATAG+GGG + chr1.4:77885991-77886010 None:intergenic 40.0%
!! CAAAAGGCATGTTTTGTGGA+TGG + chr1.4:77885220-77885239 None:intergenic 40.0%
!! TTAGGTTGATCTTGCCATTG+AGG - chr1.4:77885896-77885915 MS.gene061156:CDS 40.0%
!!! CGACCTTTTGTTATTGAACG+CGG + chr1.4:77886407-77886426 None:intergenic 40.0%
!!! GATTTTTTCTGTCACCGAGA+AGG - chr1.4:77884321-77884340 MS.gene061156:CDS 40.0%
!!! GGAGGATAGTTTTGGACAAT+AGG - chr1.4:77885670-77885689 MS.gene061156:CDS 40.0%
ACAACTTGTCCGGACAAAGA+AGG - chr1.4:77886515-77886534 MS.gene061156:intron 45.0%
CCTCCGCGTTCAATAACAAA+AGG - chr1.4:77886401-77886420 MS.gene061156:intron 45.0%
CGTAATGGCAGATACCTTGT+TGG + chr1.4:77886260-77886279 None:intergenic 45.0%
CTTCGTGGACATTTCTTTGG+GGG - chr1.4:77885457-77885476 MS.gene061156:intron 45.0%
GATCTTGCCATTGAGGACAT+TGG - chr1.4:77885903-77885922 MS.gene061156:CDS 45.0%
TCAAGATCCATGGATGACTC+AGG - chr1.4:77885177-77885196 MS.gene061156:intron 45.0%
TCATCACGACACCAACGTAA+TGG + chr1.4:77886275-77886294 None:intergenic 45.0%
TGCGTTTAAGAAGTTCCAGG+AGG - chr1.4:77886028-77886047 MS.gene061156:intron 45.0%
TTCAAGGTGTGGATGCAAAG+CGG - chr1.4:77884398-77884417 MS.gene061156:CDS 45.0%
TTGGAATCGACAATCACGGA+AGG - chr1.4:77884937-77884956 MS.gene061156:CDS 45.0%
TTGTTGCCCAATGTCCTCAA+TGG + chr1.4:77885913-77885932 None:intergenic 45.0%
! AAAGGAGTGGGTTCTCAATC+CGG - chr1.4:77884254-77884273 MS.gene061156:CDS 45.0%
! AGGAAGCACATTTGATCTGG+AGG + chr1.4:77884516-77884535 None:intergenic 45.0%
! CAAGGTATCTGCCATTACGT+TGG - chr1.4:77886261-77886280 MS.gene061156:intron 45.0%
! GATTGAGAACCCACTCCTTT+TGG + chr1.4:77884254-77884273 None:intergenic 45.0%
! GCAAGGAAGCACATTTGATC+TGG + chr1.4:77884519-77884538 None:intergenic 45.0%
! TTTGGGGGTATGACAACAAC+AGG - chr1.4:77885472-77885491 MS.gene061156:intron 45.0%
!!! CCTTTTGTTATTGAACGCGG+AGG + chr1.4:77886404-77886423 None:intergenic 45.0%
!!! ATTTTTTTATCTAATATTTT+AGG - chr1.4:77885878-77885897 MS.gene061156:CDS 5.0%
AGTTCCAGGAGGAGTTTGAG+AGG - chr1.4:77886039-77886058 MS.gene061156:intron 50.0%
CAACGGCATCTCCATACTCT+TGG + chr1.4:77884863-77884882 None:intergenic 50.0%
GCAATTGCCTGAGTCATCCA+TGG + chr1.4:77885187-77885206 None:intergenic 50.0%
GTTCCAGGAGGAGTTTGAGA+GGG - chr1.4:77886040-77886059 MS.gene061156:intron 50.0%
TCAAGGTGTGGATGCAAAGC+GGG - chr1.4:77884399-77884418 MS.gene061156:CDS 50.0%
TGATTTGAGCAACGACGAGC+AGG - chr1.4:77884138-77884157 MS.gene061156:CDS 50.0%
! AGTGGGTTCTCAATCCGGAA+AGG - chr1.4:77884259-77884278 MS.gene061156:CDS 50.0%
TTGCCCTCTCAAACTCCTCC+TGG + chr1.4:77886046-77886065 None:intergenic 55.0%
!! TGGGTACCGGCTTATCTTCG+TGG - chr1.4:77885442-77885461 MS.gene061156:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 77883793 77886625 77883793 ID=MS.gene061156
chr1.4 mRNA 77883793 77886625 77883793 ID=MS.gene061156.t1;Parent=MS.gene061156
chr1.4 exon 77886573 77886625 77886573 ID=MS.gene061156.t1.exon1;Parent=MS.gene061156.t1
chr1.4 CDS 77886573 77886625 77886573 ID=cds.MS.gene061156.t1;Parent=MS.gene061156.t1
chr1.4 exon 77885516 77886014 77885516 ID=MS.gene061156.t1.exon2;Parent=MS.gene061156.t1
chr1.4 CDS 77885516 77886014 77885516 ID=cds.MS.gene061156.t1;Parent=MS.gene061156.t1
chr1.4 exon 77884848 77885081 77884848 ID=MS.gene061156.t1.exon3;Parent=MS.gene061156.t1
chr1.4 CDS 77884848 77885081 77884848 ID=cds.MS.gene061156.t1;Parent=MS.gene061156.t1
chr1.4 exon 77883793 77884518 77883793 ID=MS.gene061156.t1.exon4;Parent=MS.gene061156.t1
chr1.4 CDS 77883793 77884518 77883793 ID=cds.MS.gene061156.t1;Parent=MS.gene061156.t1
Gene Sequence

>MS.gene061156

ATGGCAAGTGCTCAGAACTCAATCAATTGTTCTCATGTGGCAGTTATAGCACAGTAAGTAGTATAATTACTTTCTTTCAATTTTTTCACCTTTTTCTTATATTGTTCTCAACTAATTCTTTATGTCACAAATGCATATTATTGCTCTGTAATTATTTCACCCCTAATTCCTTATATACGAATTCAACAATAATATTTTGTGGATAAATAATAGTGTTATCATTTTACTTTTTAGCTATGTAGTAGTAATATGAATTTATTTGTGTTGGTTAGGAGTCACAGATTTAATTGATTTGAACAATGTTCCAAATAATGAACCATGTGATAATTCAGAAAATGAAGTAATTGATTTGAGCAACGACGAGCAGGAAACAATTACTATCAAAAATTGTTTTGAACCTTTTATTGGACAATGTTTTCTAAGTGAGGAAGAAGCTTTAGAATTTAATGAGAATTATGCCAAAAGGAGTGGGTTCTCAATCCGGAAAGGTCGATTTCTTAAAAAAAATATGGGATGATACATAGGAGGGATTTTTTCTGTCACCGAGAAGGATTTCCAGAAAAAAAATATCATGATCCATCTAAAGAACAACGAAATAGACAATCTTCAAGGTGTGGATGCAAAGCGGGCATGACAATCAAATTGAAGAAATCCTTTGAGATTTTTCCAAAAGAATGGCATGTTATTGAACTTATATCCAACCATAATCATGATTTACTACCTCCAGATCAAATGTGCTTCCTTGCATCTTACCGCAACATTTCTGAGGAAGATGAGAAGCGAATTTCGTTATTAAAAGAAGCTGATCTTTCAGTCAAGCAAATAATGCGTGTTATGGAGCTTGAGAAAAATGTTAAGTATGGTGATCTTCCATTTCTTCGGAAAGATATTCATAATTTCTTCAATAAGATTCACCAAGGTAAATTGGATGGTGATGCTGATGATCTTTTACAATTTTGTAAGTGTGCCAAGGATGAAAACTCTTGCTTCCAATTTGCATTCACTGTTGATAAAGAAAAAAGACTAGAGAATATATTTTGGTCTCCACCTCATTGCTTTGAGTTGTACCAAGAGTATGGAGATGCCGTTGCATTTGACACTACCTACAAGGTGAATTCTTATAATATGCCTTTTGGCATTTTTGTTGGAATCGACAATCACGGAAGGACAATATTGTTTGGTTGCGCGCTCATTCGAAATGAAACTTTGTCTACATTTCGATGGTTAATGAAAGTTAGTTCATATACATCTTTTTTTTTTCTATTAACTTTGGTAAACTTTTTCTTTCATCTTTTCTAATAGTATCCCCATGTATTTTCTATTTGTTTTTTTTCTTCAGACTTGTTACTCTCATGAAGAAGTCTCCAAAGACAATCATTACCGATCAAGATCCATGGATGACTCAGGCAATTGCTTTTGAAATGCCATCCACAAAACATGCCTTTTGTATATGGCATATTACTGCTAAATTTAGTGGTTGGTTTACTTCAATTCTTCGAAATCAATATTTTTCATGGTGTGCTGATTTTTATAAACTTTACAAGCTTGACAACGTTGACGATTTTGAAGAAGAATGGTTTATAATTGTTTCCAAGTACAATCTAGAAGCAAACAAACACATAATAGGCTTGTATGAACTTAAGCAACATTGGGTACCGGCTTATCTTCGTGGACATTTCTTTGGGGGTATGACAACAACAGGAAGATCAGAGAGTATAAACGGGTTTGTGAAACGCTTTACTTCATCTCGTTCAAGTTTATTTCAACTTTTCAAACAAGTAAGTATTTATATGCTAATTTAGCTTAGTCACCTTGTTAATGTTTTCACCTTGGTGTTTGAGTAGAGTAAAAACCTCCCTGTGTTTATACTATGTTTTGGAGGATAGTTTTGGACAATAGGAGCACGTGTAGTATGCATCTGTTAGGAGTTGGCTAGCATGTTTTATGTCTCTGTTAGATATTTTGCTCTTCACAAGTGTGGTTTATGCATCTGTTAGGTGCATCATCTTCAAGCTATGCATGAATAAAATTTTGTTGATTACAAAAATGTAGAAAATGATACTTTAATAACTAATGGCATGTTTAATTTTTTTATCTAATATTTTAGGTTGATCTTGCCATTGAGGACATTGGGCAACAACAACTACATCACACCATGTTGGATACATATAGGGGATCTTCACTTCGATCATTATCCCCTATAGAGGAGCAAGTTCAAAGTATTTTCACAAATTTTGCGTTTAAGAAGTTCCAGGAGGAGTTTGAGAGGGCAACTCAATACAAGATTTGTGAAGAAAAGCATGTAGAATTTACTGTGAAATATTACAAGGAGCAACATTCACAAAATCATAAAGTCTTATGGGATGGTGATGTTGTTGGTTGTAGTTGTAAGCATTTTGAGTTTTGGGGTATTCTTTGTTGTCATGTGTTGACAATATTTCTTCATAAAGATTGCTTTCAAATTCCAACAAGGTATCTGCCATTACGTTGGTGTCGTGATGAATTTCAAAGACCAGTCGAAGTAAATGTCATGCTTAGTGAAGTAGGACATAAAGGCAAGGGTCCATATATTGAGAATGTGGAGAATGCAGTAGAGTTTATTAGCAATCCTCCGCGTTCAATAACAAAAGGTCGTCCCAAAACAAAACGATCAAAAGGTGGAATCGAATTGTCAAGGAAAGCAAGAAGTTGTAGCTTTTGCAATAGAAGTGGTCACAATGTTACAACTTGTCCGGACAAAGAAGGTTATGTTTCGTCATCAACCATCAAAAAGAGGAAGAATGAAGCTCAAGCACAACAAAATTTAAATCCTATTTTATATTTAAAGTCTTAG
Protein sequence

>MS.gene061156.t1

MASAQNSINCSHVAVIAQCGCKAGMTIKLKKSFEIFPKEWHVIELISNHNHDLLPPDQMCFLASYRNISEEDEKRISLLKEADLSVKQIMRVMELEKNVKYGDLPFLRKDIHNFFNKIHQGKLDGDADDLLQFCKCAKDENSCFQFAFTVDKEKRLENIFWSPPHCFELYQEYGDAVAFDTTYKLDNVDDFEEEWFIIVSKYNLEANKHIIGLYELKQHWVPAYLRGHFFGGMTTTGRSESINGFVKRFTSSRSSLFQLFKQVDLAIEDIGQQQLHHTMLDTYRGSSLRSLSPIEEQVQSIFTNFAFKKFQEEFERATQYKICEEKHVEFTVKYYKEQHSQNHKVLWDGDVVGCSCKHFEFWGILCCHVLTIFLHKDCFQIPTRYLPLRWCRDEFQRPVEVNVMLSEVGHKGKGPYIENVENAVEFISNPPRSITKGRPKTKRSKGGIELSRKARSCSFCNRSGHNVTTCPDKEGYVSSSTIKKRKNEAQAQQNLNPILYLKS