AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061193

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061193.t1 MTR_4g131600 69.615 441 37 6 1 344 69 509 0.0 558
MS.gene061193.t1 MTR_4g131570 55.188 453 94 7 1 344 66 518 1.06e-143 420
MS.gene061193.t1 MTR_4g131580 53.289 456 109 7 1 352 66 521 7.56e-142 416
MS.gene061193.t1 MTR_1g032570 55.500 200 79 5 2 192 61 259 2.64e-63 213
MS.gene061193.t1 MTR_2g450070 57.692 208 74 3 2 195 68 275 1.01e-62 212
MS.gene061193.t1 MTR_3g106220 57.000 200 77 3 2 192 58 257 1.24e-62 212
MS.gene061193.t1 MTR_1g013170 56.158 203 80 3 2 195 55 257 1.61e-62 208
MS.gene061193.t1 MTR_2g450070 57.692 208 74 3 2 195 68 275 1.90e-62 212
MS.gene061193.t1 MTR_1g032570 54.974 191 76 5 11 192 1 190 3.90e-59 201
MS.gene061193.t1 MTR_4g121020 51.613 217 100 4 2 213 81 297 1.41e-57 199
MS.gene061193.t1 MTR_4g121020 51.613 217 100 4 2 213 81 297 1.44e-57 199
MS.gene061193.t1 MTR_2g034960 53.646 192 88 1 2 192 79 270 8.90e-57 195
MS.gene061193.t1 MTR_3g086100 47.479 238 102 7 2 230 63 286 1.01e-56 194
MS.gene061193.t1 MTR_7g117705 51.795 195 90 2 2 192 56 250 1.10e-56 192
MS.gene061193.t1 MTR_8g079940 48.744 199 95 3 2 195 75 271 1.82e-56 193
MS.gene061193.t1 MTR_1g013180 42.071 309 149 9 2 283 206 511 1.03e-55 194
MS.gene061193.t1 MTR_3g086100 46.725 229 99 7 11 230 1 215 1.99e-52 181
MS.gene061193.t1 MTR_3g102590 47.849 186 49 3 2 186 58 196 1.19e-39 141
MS.gene061193.t1 MTR_4g121020 46.277 188 96 4 31 213 6 193 2.83e-38 144
MS.gene061193.t1 MTR_3g102590 47.802 182 47 3 2 182 58 192 2.27e-37 134
MS.gene061193.t1 MTR_1g013160 48.344 151 73 2 14 159 1 151 5.44e-37 131
MS.gene061193.t1 MTR_4g021760 35.714 196 104 3 2 197 60 233 4.33e-36 134
MS.gene061193.t1 MTR_4g021790 36.150 213 108 3 2 214 59 243 5.05e-36 132
MS.gene061193.t1 MTR_4g021855 37.234 188 96 2 2 189 59 224 7.53e-34 127
MS.gene061193.t1 MTR_4g021845 39.011 182 87 4 2 182 59 217 1.95e-33 124
MS.gene061193.t1 MTR_6g045327 32.301 226 119 6 2 219 64 263 2.15e-32 125
MS.gene061193.t1 MTR_0450s0040 36.548 197 107 6 1 197 53 231 1.25e-31 122
MS.gene061193.t1 MTR_3g102600 65.333 75 26 0 1 75 8 82 3.46e-29 110
MS.gene061193.t1 MTR_3g102600 60.377 53 21 0 132 184 82 134 1.50e-15 73.6
MS.gene061193.t1 MTR_3g102600 65.333 75 26 0 1 75 8 82 5.34e-29 111
MS.gene061193.t1 MTR_3g102600 60.345 58 23 0 132 189 82 139 2.43e-17 79.3
MS.gene061193.t1 MTR_3g102600 65.333 75 26 0 1 75 57 131 1.08e-28 110
MS.gene061193.t1 MTR_3g102600 60.377 53 21 0 132 184 131 183 3.16e-15 73.9
MS.gene061193.t1 MTR_3g102600 65.333 75 26 0 1 75 57 131 1.64e-28 111
MS.gene061193.t1 MTR_3g102600 60.345 58 23 0 132 189 131 188 4.50e-17 80.1
MS.gene061193.t1 MTR_7g026400 34.536 194 116 3 1 194 36 218 1.57e-27 114
MS.gene061193.t1 MTR_8g105600 34.946 186 117 2 2 186 58 240 9.21e-25 106
MS.gene061193.t1 MTR_4g023980 33.333 156 80 3 2 156 39 171 5.83e-22 92.0
MS.gene061193.t1 MTR_8g032710 29.952 207 118 7 3 185 68 271 5.96e-19 88.6
MS.gene061193.t1 MTR_4g064730 49.474 95 43 3 109 200 104 196 2.10e-18 85.1
MS.gene061193.t1 MTR_5g055260 31.351 185 76 7 2 185 59 193 3.54e-17 80.9
MS.gene061193.t1 MTR_5g014040 34.483 145 90 3 86 225 264 408 3.34e-16 80.5
MS.gene061193.t1 MTR_7g089010 58.621 58 24 0 132 189 90 147 3.87e-16 78.6
MS.gene061193.t1 MTR_3g092780 33.803 142 73 5 2 129 102 236 7.43e-16 79.3
MS.gene061193.t1 MTR_3g092780 33.803 142 73 5 2 129 102 236 7.84e-16 79.3
MS.gene061193.t1 MTR_4g098870 30.693 202 109 5 126 303 342 536 1.36e-15 78.6
MS.gene061193.t1 MTR_4g098870 30.693 202 109 5 126 303 323 517 1.78e-15 78.2
MS.gene061193.t1 MTR_4g098870 30.693 202 109 5 126 303 303 497 1.81e-15 78.2
MS.gene061193.t1 MTR_4g098870 30.693 202 109 5 126 303 303 497 1.93e-15 78.2
MS.gene061193.t1 MTR_4g098870 38.938 113 49 3 126 225 323 428 2.21e-15 77.8
MS.gene061193.t1 MTR_3g092780 32.847 137 80 4 2 129 102 235 5.15e-15 77.0
MS.gene061193.t1 MTR_3g092780 32.847 137 80 4 2 129 102 235 5.39e-15 77.0
MS.gene061193.t1 MTR_5g014040 33.333 150 90 4 86 225 224 373 3.10e-14 74.3
MS.gene061193.t1 MTR_5g014040 33.333 150 90 4 86 225 264 413 3.43e-14 74.3
MS.gene061193.t1 MTR_7g115530 61.404 57 21 1 131 186 216 272 6.68e-14 72.0
MS.gene061193.t1 MTR_7g115530 61.404 57 21 1 131 186 216 272 1.04e-13 71.6
MS.gene061193.t1 MTR_7g115530 62.500 56 20 1 131 185 216 271 1.04e-13 72.0
MS.gene061193.t1 MTR_7g118260 31.538 130 67 3 2 125 60 173 1.14e-13 72.8
MS.gene061193.t1 MTR_7g115530 61.404 57 21 1 131 186 216 272 1.74e-13 71.6
MS.gene061193.t1 MTR_6g032990 44.944 89 46 2 129 214 38 126 1.90e-13 71.6
MS.gene061193.t1 MTR_7g118260 41.026 78 37 2 3 74 61 135 2.75e-13 71.6
MS.gene061193.t1 MTR_2g090675 36.585 123 52 4 69 191 123 219 4.02e-13 70.5
MS.gene061193.t1 MTR_1g080330 60.714 56 21 1 131 185 173 228 5.22e-13 69.3
MS.gene061193.t1 MTR_1g080330 59.649 57 22 1 131 186 173 229 6.74e-13 69.7
MS.gene061193.t1 MTR_4g078810 50.909 55 27 0 131 185 156 210 1.26e-12 68.9
MS.gene061193.t1 MTR_8g093040 25.296 253 141 6 3 255 65 269 1.55e-12 67.8
MS.gene061193.t1 MTR_6g016850 32.039 103 68 1 2 102 58 160 1.83e-12 65.5
MS.gene061193.t1 MTR_8g024260 39.024 82 43 2 3 79 83 162 2.78e-12 68.6
MS.gene061193.t1 MTR_7g115530 61.111 54 20 1 131 183 216 269 4.46e-12 66.2
MS.gene061193.t1 MTR_4g081710 49.153 59 29 1 129 186 13 71 5.34e-12 66.6
MS.gene061193.t1 MTR_1g067110 40.789 76 42 1 2 74 131 206 6.08e-12 67.4
MS.gene061193.t1 MTR_1g067110 40.789 76 42 1 2 74 131 206 6.46e-12 67.4
MS.gene061193.t1 MTR_7g098250 49.315 73 35 2 133 203 262 334 7.34e-12 67.0
MS.gene061193.t1 MTR_2g027860 55.000 60 25 2 133 191 49 107 8.37e-12 65.9
MS.gene061193.t1 MTR_7g115530 62.264 53 19 1 131 182 216 268 9.49e-12 65.5
MS.gene061193.t1 MTR_2g023580 47.959 98 40 5 132 223 54 146 1.22e-11 64.3
MS.gene061193.t1 MTR_1g093080 46.970 66 33 2 125 189 204 268 1.23e-11 65.9
MS.gene061193.t1 MTR_4g086835 48.276 58 30 0 132 189 273 330 1.34e-11 65.9
MS.gene061193.t1 MTR_2g027800 38.095 105 60 3 133 236 39 139 1.93e-11 64.7
MS.gene061193.t1 MTR_5g041350 50.847 59 28 1 129 186 11 69 2.01e-11 65.1
MS.gene061193.t1 MTR_4g061360 42.105 76 41 1 2 74 135 210 2.46e-11 65.9
MS.gene061193.t1 MTR_4g113140 31.250 112 70 3 79 189 187 292 2.94e-11 65.1
MS.gene061193.t1 MTR_2g086450 45.570 79 37 2 129 206 41 114 3.64e-11 64.3
MS.gene061193.t1 MTR_3g037390 36.364 77 46 1 2 75 70 146 4.44e-11 64.7
MS.gene061193.t1 MTR_5g017980 46.552 58 31 0 132 189 191 248 5.33e-11 63.5
MS.gene061193.t1 MTR_5g017980 46.552 58 31 0 132 189 192 249 5.47e-11 63.5
MS.gene061193.t1 MTR_0223s0040 54.545 66 29 1 132 196 17 82 5.95e-11 63.2
MS.gene061193.t1 MTR_3g450310 49.296 71 29 2 122 185 2 72 9.19e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061193.t1 AT2G25180 57.949 195 75 3 2 192 63 254 5.94e-62 209
MS.gene061193.t1 AT3G16857 50.698 215 81 3 2 192 13 226 1.05e-59 203
MS.gene061193.t1 AT3G16857 50.698 215 81 3 2 192 83 296 2.02e-59 204
MS.gene061193.t1 AT3G16857 50.698 215 81 3 2 192 83 296 3.33e-59 203
MS.gene061193.t1 AT5G58080 52.074 217 91 6 1 212 63 271 5.29e-59 202
MS.gene061193.t1 AT4G31920 55.502 209 71 6 2 207 63 252 6.17e-58 197
MS.gene061193.t1 AT1G67710 51.142 219 97 5 2 214 57 271 3.92e-57 194
MS.gene061193.t1 AT1G67710 50.476 210 94 5 11 214 1 206 8.69e-53 181
MS.gene061193.t1 AT2G01760 50.242 207 87 4 2 194 57 261 4.85e-52 177
MS.gene061193.t1 AT2G01760 50.242 207 87 4 2 194 85 289 9.80e-52 177
MS.gene061193.t1 AT2G01760 48.990 198 85 4 11 194 1 196 2.05e-47 164
MS.gene061193.t1 AT1G67710 46.032 189 92 5 32 214 13 197 2.55e-38 142
MS.gene061193.t1 AT5G49240 42.857 189 96 5 2 182 88 272 1.34e-37 137
MS.gene061193.t1 AT3G62670 40.838 191 99 4 2 185 85 268 6.09e-36 135
MS.gene061193.t1 AT1G49190 29.455 275 148 7 2 252 80 332 2.60e-35 134
MS.gene061193.t1 AT5G07210 30.417 240 121 5 3 201 71 305 1.70e-24 105
MS.gene061193.t1 AT5G07210 30.417 240 121 5 3 201 63 297 1.89e-24 105
MS.gene061193.t1 AT2G27070 31.224 237 123 7 3 201 66 300 6.26e-23 100
MS.gene061193.t1 AT5G59570 37.594 133 78 2 86 218 96 223 9.24e-19 86.3
MS.gene061193.t1 AT5G59570 37.594 133 78 2 86 218 96 223 9.24e-19 86.3
MS.gene061193.t1 AT3G46640 48.864 88 38 2 132 218 143 224 1.09e-17 83.6
MS.gene061193.t1 AT3G46640 48.864 88 38 2 132 218 143 224 1.09e-17 83.6
MS.gene061193.t1 AT5G05090 50.667 75 37 0 115 189 63 137 1.22e-17 82.4
MS.gene061193.t1 AT3G46640 48.864 88 38 2 132 218 143 224 1.63e-17 82.8
MS.gene061193.t1 AT2G40970 58.621 58 24 0 132 189 104 161 6.82e-17 79.7
MS.gene061193.t1 AT3G10760 54.545 66 30 0 124 189 96 161 1.00e-16 80.9
MS.gene061193.t1 AT1G49190 41.333 75 44 0 2 76 80 154 2.23e-16 81.3
MS.gene061193.t1 AT1G49190 30.612 147 76 6 124 252 409 547 1.42e-11 66.2
MS.gene061193.t1 AT1G68210 25.676 222 131 4 4 192 61 281 5.86e-16 80.1
MS.gene061193.t1 AT5G44190 61.818 55 21 0 131 185 145 199 2.28e-15 77.0
MS.gene061193.t1 AT5G44190 61.818 55 21 0 131 185 145 199 2.53e-15 77.0
MS.gene061193.t1 AT2G20570 37.600 125 61 4 79 191 92 211 3.00e-13 70.9
MS.gene061193.t1 AT5G24470 39.744 78 42 2 3 76 97 173 4.55e-13 70.9
MS.gene061193.t1 AT2G46790 41.558 77 40 2 2 74 83 158 4.73e-13 70.5
MS.gene061193.t1 AT2G46790 41.558 77 40 2 2 74 83 158 5.11e-13 70.5
MS.gene061193.t1 AT5G45580 50.769 65 31 1 133 196 7 71 1.39e-12 67.8
MS.gene061193.t1 AT5G45580 50.769 65 31 1 133 196 23 87 1.50e-12 67.8
MS.gene061193.t1 AT2G20570 59.259 54 22 0 138 191 174 227 2.19e-12 68.6
MS.gene061193.t1 AT2G01060 55.000 60 25 2 133 191 15 73 6.75e-12 65.9
MS.gene061193.t1 AT4G37180 51.667 60 28 1 131 189 208 267 3.19e-11 64.7
MS.gene061193.t1 AT4G37180 51.667 60 28 1 131 189 215 274 3.61e-11 64.3
MS.gene061193.t1 AT5G06800 50.000 66 31 2 133 197 192 256 4.14e-11 63.5
MS.gene061193.t1 AT3G04030 50.847 59 28 1 129 186 41 99 4.43e-11 64.3
MS.gene061193.t1 AT3G04030 50.847 59 28 1 129 186 41 99 4.60e-11 64.3
MS.gene061193.t1 AT4G18020 54.902 51 23 0 135 185 298 348 6.03e-11 64.3
MS.gene061193.t1 AT4G18020 54.902 51 23 0 135 185 298 348 6.03e-11 64.3
MS.gene061193.t1 AT4G18020 54.902 51 23 0 135 185 298 348 6.03e-11 64.3
MS.gene061193.t1 AT4G18020 54.902 51 23 0 135 185 298 348 6.03e-11 64.3
MS.gene061193.t1 AT4G18020 54.902 51 23 0 135 185 298 348 6.03e-11 64.3
MS.gene061193.t1 AT4G18020 54.902 51 23 0 135 185 298 348 6.03e-11 64.3
MS.gene061193.t1 AT4G18020 54.902 51 23 0 135 185 298 348 6.52e-11 64.3
MS.gene061193.t1 AT4G18020 54.902 51 23 0 135 185 298 348 6.52e-11 64.3
MS.gene061193.t1 AT4G18020 54.902 51 23 0 135 185 298 348 6.52e-11 64.3
MS.gene061193.t1 AT4G18020 54.902 51 23 0 135 185 298 348 6.52e-11 64.3
MS.gene061193.t1 AT4G18020 54.902 51 23 0 135 185 298 348 6.52e-11 64.3
MS.gene061193.t1 AT5G18240 52.542 59 27 1 129 186 41 99 7.33e-11 63.5
MS.gene061193.t1 AT5G18240 52.542 59 27 1 129 186 41 99 7.33e-11 63.5
MS.gene061193.t1 AT5G60100 42.857 77 41 1 1 74 109 185 7.49e-11 63.5
MS.gene061193.t1 AT5G60100 42.857 77 41 1 1 74 109 185 7.49e-11 63.5
MS.gene061193.t1 AT5G60100 42.857 77 41 1 1 74 109 185 7.49e-11 63.5
MS.gene061193.t1 AT5G18240 52.542 59 27 1 129 186 41 99 7.84e-11 63.5
MS.gene061193.t1 AT5G18240 52.542 59 27 1 129 186 41 99 8.03e-11 63.5
MS.gene061193.t1 AT5G18240 52.542 59 27 1 129 186 41 99 8.03e-11 63.5
MS.gene061193.t1 AT5G60100 42.857 77 41 1 1 74 109 185 8.81e-11 63.5
MS.gene061193.t1 AT5G60100 42.857 77 41 1 1 74 109 185 8.81e-11 63.5
MS.gene061193.t1 AT5G60100 42.857 77 41 1 1 74 109 185 8.81e-11 63.5
MS.gene061193.t1 AT5G29000 41.975 81 46 1 106 185 204 284 9.08e-11 62.8
MS.gene061193.t1 AT5G06800 50.000 66 31 2 133 197 192 256 9.36e-11 63.2

Find 74 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACATTGGTGTCTCGAAAT+TGG 0.205330 8.4:-1955741 None:intergenic
GATTATCTTGCTTCTTCTAA+TGG 0.232806 8.4:+1956297 MS.gene061193:CDS
CCACTTCTGACAGGGTTAAA+AGG 0.242635 8.4:-1956040 None:intergenic
AAAAGAAGCCTCGTTTAGTT+TGG 0.279239 8.4:+1955092 MS.gene061193:CDS
CAATGTCAAGATGGCTTACT+TGG 0.323761 8.4:+1956208 MS.gene061193:CDS
CTCACTCAATGGAACAGAAT+TGG 0.343212 8.4:-1956080 None:intergenic
ATCATTAGTGATGGGGTATA+TGG 0.343848 8.4:+1954953 MS.gene061193:CDS
AAAGAAGCCTCGTTTAGTTT+GGG 0.355798 8.4:+1955093 MS.gene061193:CDS
AGACATCGTTGCTGTTCCTT+TGG 0.355803 8.4:+1956174 MS.gene061193:CDS
GTTTCTGCAGTTAATCTTGT+CGG 0.381222 8.4:+1955139 MS.gene061193:CDS
TATCAAATGGCTATCTAAAC+AGG 0.381598 8.4:+1955534 MS.gene061193:CDS
CATCGTTGCTGTTCCTTTGG+AGG 0.383670 8.4:+1956177 MS.gene061193:CDS
AACATTGGTGTCTCGAAATT+GGG 0.390536 8.4:-1955740 None:intergenic
TCAGCAAGTCGGACTTGAAA+TGG 0.400458 8.4:+1954667 MS.gene061193:CDS
AAATGTATCCAGCCATTTGC+AGG 0.408981 8.4:+1955336 MS.gene061193:CDS
CCACCTACTGAACCCTTTGC+TGG 0.411244 8.4:-1955584 None:intergenic
TATATATTTGTACCTGCAAA+TGG 0.440275 8.4:-1955348 None:intergenic
AGCTTGAAGCCATCCATGTC+TGG 0.455651 8.4:-1954641 None:intergenic
AGAATTGGAAGCAAAGTTAG+AGG 0.465879 8.4:-1956065 None:intergenic
CATTGCATCGTTTCCTCCAA+AGG 0.471428 8.4:-1956190 None:intergenic
GATACAGAATTGATATCAAA+TGG 0.472061 8.4:+1955521 MS.gene061193:CDS
TGACAGTGGCTCTGATGTCT+TGG 0.472341 8.4:-1955906 None:intergenic
CTTGACTTGATGAATGTTGA+AGG 0.474751 8.4:+1955301 MS.gene061193:CDS
TTAAGCTCTTCCATTCGAAT+CGG 0.481435 8.4:-1954872 None:intergenic
TTCCTCCTTCAACAATACCT+TGG 0.487555 8.4:+1956275 MS.gene061193:CDS
TATTTGTACCTGCAAATGGC+TGG 0.488638 8.4:-1955344 None:intergenic
TGGAAGAGCTTAAGAGCATA+TGG 0.489491 8.4:+1954882 MS.gene061193:CDS
TGAATGTTGAAGGGCTTACA+AGG 0.497287 8.4:+1955311 MS.gene061193:CDS
TTGTTAAAATCCTCACTCAA+TGG 0.497939 8.4:-1956091 None:intergenic
TAGAAAGAAGATTGAAAGCA+AGG 0.500776 8.4:+1954916 MS.gene061193:CDS
TTAGAGGAACCACTTCTGAC+AGG 0.501081 8.4:-1956049 None:intergenic
ATAATCCAAGGTATTGTTGA+AGG 0.508220 8.4:-1956280 None:intergenic
ATCAAGGTATCATTAGTGAT+GGG 0.510346 8.4:+1954945 MS.gene061193:CDS
AATCAAGGTATCATTAGTGA+TGG 0.511362 8.4:+1954944 MS.gene061193:CDS
TGTGTGATTTATAGTGTTGT+CGG 0.513795 8.4:+1954784 MS.gene061193:intron
GAAAGCAAGGATCAAAATCA+AGG 0.515217 8.4:+1954929 MS.gene061193:CDS
AATGCAGTTACAGATTCCTC+TGG 0.518088 8.4:+1955880 MS.gene061193:intron
ACTTTCACTTTCAGAAACAT+TGG 0.524736 8.4:-1955755 None:intergenic
TGGCTATCTAAACAGGATAG+AGG 0.531559 8.4:+1955541 MS.gene061193:CDS
CAATTCTGTTCCATTGAGTG+AGG 0.541688 8.4:+1956081 MS.gene061193:CDS
TGCTAACATCACTAATTACA+AGG 0.543736 8.4:-1954616 None:intergenic
CGTTGGATCCTACCAGCAAA+GGG 0.544495 8.4:+1955572 MS.gene061193:CDS
TAATGGAGACACTAGTTCCA+TGG 0.565315 8.4:+1956314 MS.gene061193:CDS
GCGTTGGATCCTACCAGCAA+AGG 0.570938 8.4:+1955571 MS.gene061193:CDS
TTTCAAGTCCGACTTGCTGA+AGG 0.572445 8.4:-1954664 None:intergenic
CTACTGAACCCTTTGCTGGT+AGG 0.574976 8.4:-1955580 None:intergenic
TTGACTTGATGAATGTTGAA+GGG 0.580486 8.4:+1955302 MS.gene061193:CDS
GAAACGATGCAATGTCAAGA+TGG 0.582127 8.4:+1956199 MS.gene061193:CDS
GGATAGAGGAGATGATGCGT+TGG 0.582696 8.4:+1955555 MS.gene061193:CDS
TTGGGCTAAACTTTGTACCA+TGG 0.585108 8.4:-1956331 None:intergenic
AAGAAAGGTACAAAGTGAGG+AGG 0.585901 8.4:+1955030 MS.gene061193:CDS
GAAAGTTCGAGTTCTTTACA+TGG 0.585974 8.4:+1955772 MS.gene061193:CDS
TTCAAGCTCCTTCAGCAAGT+CGG 0.589889 8.4:+1954656 MS.gene061193:CDS
AACATTTGCATTATTGACAG+TGG 0.591551 8.4:-1955920 None:intergenic
AGAAGAAGCAAGATAATCCA+AGG 0.597269 8.4:-1956292 None:intergenic
TGATGTTAGCATGCCAGACA+TGG 0.598210 8.4:+1954628 MS.gene061193:CDS
TCTAGTGAAACCGATTCGAA+TGG 0.598674 8.4:+1954862 MS.gene061193:CDS
ATGTTATGAGACTGAAGTGG+AGG 0.606395 8.4:-1955716 None:intergenic
GCTAACAAGAATAAAACGCA+TGG 0.616521 8.4:+1955001 MS.gene061193:CDS
GGTAAATGATGATATAGAGA+AGG 0.617327 8.4:+1954805 MS.gene061193:CDS
AATGTCAAGATGGCTTACTT+GGG 0.618703 8.4:+1956209 MS.gene061193:CDS
TAGAGGAACCACTTCTGACA+GGG 0.620617 8.4:-1956048 None:intergenic
ATCCAAGGTATTGTTGAAGG+AGG 0.626426 8.4:-1956277 None:intergenic
GTCACATAAACTCACTGTCA+AGG 0.629956 8.4:-1955162 None:intergenic
TCAAGGTATCATTAGTGATG+GGG 0.639460 8.4:+1954946 MS.gene061193:CDS
TGTGTGAGCTTACTGATGAA+AGG 0.640199 8.4:-1954695 None:intergenic
TCTGATGTCTTGGAAGCCAG+AGG 0.643398 8.4:-1955896 None:intergenic
GTTAGCATGCCAGACATGGA+TGG 0.657571 8.4:+1954632 MS.gene061193:CDS
TGAGCTTACTGATGAAAGGT+AGG 0.657693 8.4:-1954691 None:intergenic
GTGATGAAGAGTGTGATACA+TGG 0.658673 8.4:+1954827 MS.gene061193:CDS
TGTTATGAGACTGAAGTGGA+GGG 0.664363 8.4:-1955715 None:intergenic
GGAATGTTATGAGACTGAAG+TGG 0.672395 8.4:-1955719 None:intergenic
GAATGTTGAAGGGCTTACAA+GGG 0.675399 8.4:+1955312 MS.gene061193:CDS
TCATGATCCCAAACTAAACG+AGG 0.706682 8.4:-1955100 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATCCGAATATAAGAAA+TGG - chr8.4:1954760-1954779 None:intergenic 20.0%
!! ATAAGAAATGGATCTTAATT+AGG - chr8.4:1954748-1954767 None:intergenic 20.0%
!! TAAGATCCATTTCTTATATT+CGG + chr8.4:1954751-1954770 MS.gene061193:intron 20.0%
!! TGATGAATGATTCTTAAATA+TGG + chr8.4:1955214-1955233 MS.gene061193:intron 20.0%
!!! ATTCTTGTTAGCAATATTTT+CGG - chr8.4:1954993-1955012 None:intergenic 20.0%
! AGTTGAAGAAACTACATATT+AGG - chr8.4:1955251-1955270 None:intergenic 25.0%
! CTTAAATTAATGTTATCAGG+CGG - chr8.4:1955844-1955863 None:intergenic 25.0%
! GATACAGAATTGATATCAAA+TGG + chr8.4:1955521-1955540 MS.gene061193:CDS 25.0%
! GGACTTAAATTAATGTTATC+AGG - chr8.4:1955847-1955866 None:intergenic 25.0%
! TATATATTTGTACCTGCAAA+TGG - chr8.4:1955351-1955370 None:intergenic 25.0%
!! ATCAAGTCAAGTATTTTCTT+AGG - chr8.4:1955292-1955311 None:intergenic 25.0%
!!! AGTTTGAGATTTTTTGACAA+AGG - chr8.4:1955382-1955401 None:intergenic 25.0%
!!! CAAAACAGATTTTAAGAGAA+AGG - chr8.4:1954721-1954740 None:intergenic 25.0%
!!! GTTTGAGATTTTTTGACAAA+GGG - chr8.4:1955381-1955400 None:intergenic 25.0%
AACATTTGCATTATTGACAG+TGG - chr8.4:1955923-1955942 None:intergenic 30.0%
ACTGCATTTGAATCATCAAT+TGG - chr8.4:1955868-1955887 None:intergenic 30.0%
ACTTTCACTTTCAGAAACAT+TGG - chr8.4:1955758-1955777 None:intergenic 30.0%
GATAAATATTCTGCTGAAAC+TGG - chr8.4:1955822-1955841 None:intergenic 30.0%
GATTATCTTGCTTCTTCTAA+TGG + chr8.4:1956297-1956316 MS.gene061193:CDS 30.0%
GGTAAATGATGATATAGAGA+AGG + chr8.4:1954805-1954824 MS.gene061193:CDS 30.0%
TAGAAAGAAGATTGAAAGCA+AGG + chr8.4:1954916-1954935 MS.gene061193:CDS 30.0%
TATCAAATGGCTATCTAAAC+AGG + chr8.4:1955534-1955553 MS.gene061193:CDS 30.0%
TGCAATCGAAAATGAAAATG+AGG - chr8.4:1955499-1955518 None:intergenic 30.0%
TGCTAACATCACTAATTACA+AGG - chr8.4:1954619-1954638 None:intergenic 30.0%
TGTGTGATTTATAGTGTTGT+CGG + chr8.4:1954784-1954803 MS.gene061193:intron 30.0%
TTCTTCAACTATTATTGCAG+AGG + chr8.4:1955261-1955280 MS.gene061193:intron 30.0%
TTGTTAAAATCCTCACTCAA+TGG - chr8.4:1956094-1956113 None:intergenic 30.0%
TTTCAACCGTATTTGCAAAA+AGG - chr8.4:1955429-1955448 None:intergenic 30.0%
! AAAAAGAAAGGTACAAAGTG+AGG + chr8.4:1955027-1955046 MS.gene061193:CDS 30.0%
! AATCAAGGTATCATTAGTGA+TGG + chr8.4:1954944-1954963 MS.gene061193:CDS 30.0%
! ATCAAGGTATCATTAGTGAT+GGG + chr8.4:1954945-1954964 MS.gene061193:CDS 30.0%
! TTGACTTGATGAATGTTGAA+GGG + chr8.4:1955302-1955321 MS.gene061193:CDS 30.0%
!! ATAATCCAAGGTATTGTTGA+AGG - chr8.4:1956283-1956302 None:intergenic 30.0%
!! ATTTTGATGTGTGAGTAACA+TGG - chr8.4:1956250-1956269 None:intergenic 30.0%
AAAAGAAGCCTCGTTTAGTT+TGG + chr8.4:1955092-1955111 MS.gene061193:CDS 35.0%
AACGCATGGACAAAAAAGAA+AGG + chr8.4:1955015-1955034 MS.gene061193:CDS 35.0%
AATGTCAAGATGGCTTACTT+GGG + chr8.4:1956209-1956228 MS.gene061193:CDS 35.0%
AGAAGAAGCAAGATAATCCA+AGG - chr8.4:1956295-1956314 None:intergenic 35.0%
AGAATTGGAAGCAAAGTTAG+AGG - chr8.4:1956068-1956087 None:intergenic 35.0%
ATAAATCACTCTCCTGTTGA+AGG + chr8.4:1955997-1956016 MS.gene061193:intron 35.0%
GAAAGCAAGGATCAAAATCA+AGG + chr8.4:1954929-1954948 MS.gene061193:CDS 35.0%
GCTAACAAGAATAAAACGCA+TGG + chr8.4:1955001-1955020 MS.gene061193:CDS 35.0%
GTCGAAGAAAAATGAGAACT+GGG - chr8.4:1956154-1956173 None:intergenic 35.0%
GTTTCTGCAGTTAATCTTGT+CGG + chr8.4:1955139-1955158 MS.gene061193:CDS 35.0%
TAAATCACTCTCCTGTTGAA+GGG + chr8.4:1955998-1956017 MS.gene061193:intron 35.0%
TGTCGAAGAAAAATGAGAAC+TGG - chr8.4:1956155-1956174 None:intergenic 35.0%
TTAAGCTCTTCCATTCGAAT+CGG - chr8.4:1954875-1954894 None:intergenic 35.0%
! AAACATTGGTGTCTCGAAAT+TGG - chr8.4:1955744-1955763 None:intergenic 35.0%
! AAAGAAGCCTCGTTTAGTTT+GGG + chr8.4:1955093-1955112 MS.gene061193:CDS 35.0%
! AACATTGGTGTCTCGAAATT+GGG - chr8.4:1955743-1955762 None:intergenic 35.0%
! ATCATTAGTGATGGGGTATA+TGG + chr8.4:1954953-1954972 MS.gene061193:CDS 35.0%
! CATCCAATGACATGTTTTGT+CGG + chr8.4:1955639-1955658 MS.gene061193:intron 35.0%
! CTTGACTTGATGAATGTTGA+AGG + chr8.4:1955301-1955320 MS.gene061193:CDS 35.0%
! GAAAGTTCGAGTTCTTTACA+TGG + chr8.4:1955772-1955791 MS.gene061193:CDS 35.0%
! GTTAGCAATATTTTCGGATG+AGG - chr8.4:1954987-1955006 None:intergenic 35.0%
! TCAAGGTATCATTAGTGATG+GGG + chr8.4:1954946-1954965 MS.gene061193:CDS 35.0%
! TCACCTCCTTTTTGCAAATA+CGG + chr8.4:1955420-1955439 MS.gene061193:intron 35.0%
AAATGTATCCAGCCATTTGC+AGG + chr8.4:1955336-1955355 MS.gene061193:CDS 40.0%
AATGCAGTTACAGATTCCTC+TGG + chr8.4:1955880-1955899 MS.gene061193:intron 40.0%
ACATGGCATTCAAACGTCAA+TGG + chr8.4:1955789-1955808 MS.gene061193:intron 40.0%
ATGTCATTGGATGCATATGC+TGG - chr8.4:1955632-1955651 None:intergenic 40.0%
ATGTTATGAGACTGAAGTGG+AGG - chr8.4:1955719-1955738 None:intergenic 40.0%
CAACCGTATTTGCAAAAAGG+AGG - chr8.4:1955426-1955445 None:intergenic 40.0%
CAATGTCAAGATGGCTTACT+TGG + chr8.4:1956208-1956227 MS.gene061193:CDS 40.0%
CAATTCTGTTCCATTGAGTG+AGG + chr8.4:1956081-1956100 MS.gene061193:CDS 40.0%
CATTCCTTCCAAGTTCAAAC+TGG - chr8.4:1955678-1955697 None:intergenic 40.0%
CTCACTCAATGGAACAGAAT+TGG - chr8.4:1956083-1956102 None:intergenic 40.0%
GAAACGATGCAATGTCAAGA+TGG + chr8.4:1956199-1956218 MS.gene061193:CDS 40.0%
GAATGTTGAAGGGCTTACAA+GGG + chr8.4:1955312-1955331 MS.gene061193:CDS 40.0%
GGAATGTTATGAGACTGAAG+TGG - chr8.4:1955722-1955741 None:intergenic 40.0%
GTCACATAAACTCACTGTCA+AGG - chr8.4:1955165-1955184 None:intergenic 40.0%
TAATGGAGACACTAGTTCCA+TGG + chr8.4:1956314-1956333 MS.gene061193:CDS 40.0%
TATTTGTACCTGCAAATGGC+TGG - chr8.4:1955347-1955366 None:intergenic 40.0%
TCATGATCCCAAACTAAACG+AGG - chr8.4:1955103-1955122 None:intergenic 40.0%
TGAATGTTGAAGGGCTTACA+AGG + chr8.4:1955311-1955330 MS.gene061193:CDS 40.0%
TGAGCTTACTGATGAAAGGT+AGG - chr8.4:1954694-1954713 None:intergenic 40.0%
TGGCTATCTAAACAGGATAG+AGG + chr8.4:1955541-1955560 MS.gene061193:CDS 40.0%
TGTGTGAGCTTACTGATGAA+AGG - chr8.4:1954698-1954717 None:intergenic 40.0%
TGTTATGAGACTGAAGTGGA+GGG - chr8.4:1955718-1955737 None:intergenic 40.0%
TTCCTCCTTCAACAATACCT+TGG + chr8.4:1956275-1956294 MS.gene061193:CDS 40.0%
! AAGAAAGGTACAAAGTGAGG+AGG + chr8.4:1955030-1955049 MS.gene061193:CDS 40.0%
! ACTAAACGAGGCTTCTTTTG+AGG - chr8.4:1955091-1955110 None:intergenic 40.0%
! GTGATGAAGAGTGTGATACA+TGG + chr8.4:1954827-1954846 MS.gene061193:CDS 40.0%
! TGGAAGAGCTTAAGAGCATA+TGG + chr8.4:1954882-1954901 MS.gene061193:CDS 40.0%
!! ATCCAAGGTATTGTTGAAGG+AGG - chr8.4:1956280-1956299 None:intergenic 40.0%
!! TCTAGTGAAACCGATTCGAA+TGG + chr8.4:1954862-1954881 MS.gene061193:CDS 40.0%
AAGGGTTCAGTAGGTGGATA+TGG + chr8.4:1955590-1955609 MS.gene061193:intron 45.0%
AGGGTTCAGTAGGTGGATAT+GGG + chr8.4:1955591-1955610 MS.gene061193:intron 45.0%
CACACCATCCAGTTTGAACT+TGG + chr8.4:1955667-1955686 MS.gene061193:intron 45.0%
CAGCCGACAAAACATGTCAT+TGG - chr8.4:1955645-1955664 None:intergenic 45.0%
CATTGCATCGTTTCCTCCAA+AGG - chr8.4:1956193-1956212 None:intergenic 45.0%
CCACTTCTGACAGGGTTAAA+AGG - chr8.4:1956043-1956062 None:intergenic 45.0%
CCATCCAGTTTGAACTTGGA+AGG + chr8.4:1955671-1955690 MS.gene061193:intron 45.0%
CCTTCCAAGTTCAAACTGGA+TGG - chr8.4:1955674-1955693 None:intergenic 45.0%
TAGAGGAACCACTTCTGACA+GGG - chr8.4:1956051-1956070 None:intergenic 45.0%
TCAGCAAGTCGGACTTGAAA+TGG + chr8.4:1954667-1954686 MS.gene061193:CDS 45.0%
TGATGTTAGCATGCCAGACA+TGG + chr8.4:1954628-1954647 MS.gene061193:CDS 45.0%
TTAGAGGAACCACTTCTGAC+AGG - chr8.4:1956052-1956071 None:intergenic 45.0%
TTCAAGCTCCTTCAGCAAGT+CGG + chr8.4:1954656-1954675 MS.gene061193:CDS 45.0%
! AGACATCGTTGCTGTTCCTT+TGG + chr8.4:1956174-1956193 MS.gene061193:CDS 45.0%
! CCTTTTAACCCTGTCAGAAG+TGG + chr8.4:1956040-1956059 MS.gene061193:CDS 45.0%
!! TTTCAAGTCCGACTTGCTGA+AGG - chr8.4:1954667-1954686 None:intergenic 45.0%
AGCTTGAAGCCATCCATGTC+TGG - chr8.4:1954644-1954663 None:intergenic 50.0%
CGTTGGATCCTACCAGCAAA+GGG + chr8.4:1955572-1955591 MS.gene061193:CDS 50.0%
CTACCAGCAAAGGGTTCAGT+AGG + chr8.4:1955581-1955600 MS.gene061193:intron 50.0%
CTACTGAACCCTTTGCTGGT+AGG - chr8.4:1955583-1955602 None:intergenic 50.0%
GGATAGAGGAGATGATGCGT+TGG + chr8.4:1955555-1955574 MS.gene061193:CDS 50.0%
GGGTTCAGTAGGTGGATATG+GGG + chr8.4:1955592-1955611 MS.gene061193:intron 50.0%
GTTAGCATGCCAGACATGGA+TGG + chr8.4:1954632-1954651 MS.gene061193:CDS 50.0%
TCACAGCAGAACCCTTCAAC+AGG - chr8.4:1956012-1956031 None:intergenic 50.0%
TCTGATGTCTTGGAAGCCAG+AGG - chr8.4:1955899-1955918 None:intergenic 50.0%
! CATCGTTGCTGTTCCTTTGG+AGG + chr8.4:1956177-1956196 MS.gene061193:CDS 50.0%
!! TGACAGTGGCTCTGATGTCT+TGG - chr8.4:1955909-1955928 None:intergenic 50.0%
CCACCTACTGAACCCTTTGC+TGG - chr8.4:1955587-1955606 None:intergenic 55.0%
CCAGCAAAGGGTTCAGTAGG+TGG + chr8.4:1955584-1955603 MS.gene061193:intron 55.0%
GCGTTGGATCCTACCAGCAA+AGG + chr8.4:1955571-1955590 MS.gene061193:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 1954608 1956348 1954608 ID=MS.gene061193
chr8.4 mRNA 1954608 1956348 1954608 ID=MS.gene061193.t1;Parent=MS.gene061193
chr8.4 exon 1954608 1954704 1954608 ID=MS.gene061193.t1.exon1;Parent=MS.gene061193.t1
chr8.4 CDS 1954608 1954704 1954608 ID=cds.MS.gene061193.t1;Parent=MS.gene061193.t1
chr8.4 exon 1954798 1955169 1954798 ID=MS.gene061193.t1.exon2;Parent=MS.gene061193.t1
chr8.4 CDS 1954798 1955169 1954798 ID=cds.MS.gene061193.t1;Parent=MS.gene061193.t1
chr8.4 exon 1955281 1955357 1955281 ID=MS.gene061193.t1.exon3;Parent=MS.gene061193.t1
chr8.4 CDS 1955281 1955357 1955281 ID=cds.MS.gene061193.t1;Parent=MS.gene061193.t1
chr8.4 exon 1955520 1955598 1955520 ID=MS.gene061193.t1.exon4;Parent=MS.gene061193.t1
chr8.4 CDS 1955520 1955598 1955520 ID=cds.MS.gene061193.t1;Parent=MS.gene061193.t1
chr8.4 exon 1955693 1955803 1955693 ID=MS.gene061193.t1.exon5;Parent=MS.gene061193.t1
chr8.4 CDS 1955693 1955803 1955693 ID=cds.MS.gene061193.t1;Parent=MS.gene061193.t1
chr8.4 exon 1955887 1955939 1955887 ID=MS.gene061193.t1.exon6;Parent=MS.gene061193.t1
chr8.4 CDS 1955887 1955939 1955887 ID=cds.MS.gene061193.t1;Parent=MS.gene061193.t1
chr8.4 exon 1956019 1956348 1956019 ID=MS.gene061193.t1.exon7;Parent=MS.gene061193.t1
chr8.4 CDS 1956019 1956348 1956019 ID=cds.MS.gene061193.t1;Parent=MS.gene061193.t1
Gene Sequence

>MS.gene061193

ATGTTTGACCTTGTAATTAGTGATGTTAGCATGCCAGACATGGATGGCTTCAAGCTCCTTCAGCAAGTCGGACTTGAAATGGACCTACCTTTCATCAGTAAGCTCACACACCTTTCTCTTAAAATCTGTTTTGTTTACCTAATTAAGATCCATTTCTTATATTCGGATATTTTGTGTGTGTGATTTATAGTGTTGTCGGTAAATGATGATATAGAGAAGGTGATGAAGAGTGTGATACATGGTGCTTGCAACTATCTAGTGAAACCGATTCGAATGGAAGAGCTTAAGAGCATATGGCAACATGTAGTTAGAAAGAAGATTGAAAGCAAGGATCAAAATCAAGGTATCATTAGTGATGGGGTATATGGTCAAGACACCTCATCCGAAAATATTGCTAACAAGAATAAAACGCATGGACAAAAAAGAAAGGTACAAAGTGAGGAGGAAGAAGCAGAACAAGATAATGATGAGAAACGTTCGCCTCAAAAGAAGCCTCGTTTAGTTTGGGATCATGAGTTGCATCGAAAATTTGTTTCTGCAGTTAATCTTGTCGGCCTTGACAGTGAGTTTATGTGACAATGAAATTATTTTTTTACTTTGCTAATTTGATGAATGATTCTTAAATATGGATATTATTGTTCCTAATATGTAGTTTCTTCAACTATTATTGCAGAGGCTTCGCCTAAGAAAATACTTGACTTGATGAATGTTGAAGGGCTTACAAGGGAAAATGTATCCAGCCATTTGCAGGTACAAATATATATAACTTTCCCTTTGTCAAAAAATCTCAAACTAGTTTATCATTAAAATATTCACCTCCTTTTTGCAAATACGGTTGAAACTTCAACAAGCAAGTTTATGAAACGTAAGTATAAAAACTGTGTGTAACCTCATTTTCATTTTCGATTGCAGAGATACAGAATTGATATCAAATGGCTATCTAAACAGGATAGAGGAGATGATGCGTTGGATCCTACCAGCAAAGGGTTCAGTAGGTGGATATGGGGATTTTTGCACACTGCCAGCATATGCATCCAATGACATGTTTTGTCGGCTGAACACACCATCCAGTTTGAACTTGGAAGGAATGAGTTCTTCTGCACTAGTCCCTCCACTTCAGTCTCATAACATTCCCAATTTCGAGACACCAATGTTTCTGAAAGTGAAAGTTCGAGTTCTTTACATGGCATTCAAACGTCAATGGAAATTAACCAGTTTCAGCAGAATATTTATCCGCCTGATAACATTAATTTAAGTCCAATTGATGATTCAAATGCAGTTACAGATTCCTCTGGCTTCCAAGACATCAGAGCCACTGTCAATAATGCAAATGTTTCTCTTTCTTGCATCGCAAGCAATCACATACAAACACATAAATCAGAAGCATTTATAAATCACTCTCCTGTTGAAGGGTTCTGCTGTGAAAACACAACCTTTTAACCCTGTCAGAAGTGGTTCCTCTAACTTTGCTTCCAATTCTGTTCCATTGAGTGAGGATTTTAACAATGATCAAATGTTGCACAATAACTACAAGTTTACTTCTCCCAGTTCTCATTTTTCTTCGACAGACATCGTTGCTGTTCCTTTGGAGGAAACGATGCAATGTCAAGATGGCTTACTTGGGAATGTTATAAATGCTCCATGTTACTCACACATCAAAATGCGAGTTCCTCCTTCAACAATACCTTGGATTATCTTGCTTCTTCTAATGGAGACACTAGTTCCATGGTACAAAGTTTAG
Protein sequence

>MS.gene061193.t1

MFDLVISDVSMPDMDGFKLLQQVGLEMDLPFIMLSVNDDIEKVMKSVIHGACNYLVKPIRMEELKSIWQHVVRKKIESKDQNQGIISDGVYGQDTSSENIANKNKTHGQKRKVQSEEEEAEQDNDEKRSPQKKPRLVWDHELHRKFVSAVNLVGLDKASPKKILDLMNVEGLTRENVSSHLQRYRIDIKWLSKQDRGDDALDPTSKGFRMSSSALVPPLQSHNIPNFETPMFLKVKVRVLYMAFKLTDSSGFQDIRATVNNANGSAVKTQPFNPVRSGSSNFASNSVPLSEDFNNDQMLHNNYKFTSPSSHFSSTDIVAVPLEETMQCQDGLLGNVINAPCYSHIKMRVPPSTIPWIILLLLMETLVPWYKV