AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061197

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061197.t1 MTR_4g131600 91.141 587 41 1 1 576 4 590 0.0 1062
MS.gene061197.t1 MTR_4g131570 70.451 599 151 4 1 576 1 596 0.0 821
MS.gene061197.t1 MTR_4g131580 69.831 590 159 4 1 571 1 590 0.0 798
MS.gene061197.t1 MTR_2g450070 40.516 659 288 19 2 567 3 650 1.01e-122 377
MS.gene061197.t1 MTR_2g450070 40.516 659 288 19 2 567 3 650 2.85e-122 376
MS.gene061197.t1 MTR_3g106220 52.147 326 140 6 14 323 5 330 9.89e-93 298
MS.gene061197.t1 MTR_1g013170 48.378 339 144 6 13 323 1 336 5.79e-92 291
MS.gene061197.t1 MTR_1g032570 55.200 250 102 5 17 257 11 259 7.98e-84 274
MS.gene061197.t1 MTR_4g121020 44.959 367 166 10 14 350 25 385 4.08e-81 268
MS.gene061197.t1 MTR_4g121020 44.959 367 166 10 14 350 25 385 4.21e-81 268
MS.gene061197.t1 MTR_1g013180 48.000 325 141 6 13 312 152 473 1.16e-80 267
MS.gene061197.t1 MTR_3g086100 54.582 251 101 5 17 258 13 259 2.12e-80 263
MS.gene061197.t1 MTR_8g079940 49.254 268 120 5 15 277 23 279 9.88e-78 255
MS.gene061197.t1 MTR_7g117705 46.254 307 158 4 13 312 1 307 2.69e-77 252
MS.gene061197.t1 MTR_2g034960 43.353 346 184 4 11 350 23 362 6.85e-77 255
MS.gene061197.t1 MTR_3g102590 50.000 236 70 3 17 251 8 196 2.38e-60 200
MS.gene061197.t1 MTR_1g032570 54.974 191 76 5 76 257 1 190 5.36e-58 203
MS.gene061197.t1 MTR_3g102590 50.000 232 68 3 17 247 8 192 1.24e-57 191
MS.gene061197.t1 MTR_3g086100 51.042 192 81 5 76 258 1 188 8.96e-51 182
MS.gene061197.t1 MTR_4g021760 37.872 235 123 4 16 250 10 221 3.19e-49 173
MS.gene061197.t1 MTR_4g021790 37.500 256 131 4 17 272 10 236 2.30e-47 167
MS.gene061197.t1 MTR_3g102600 60.769 130 51 0 11 140 2 131 2.24e-46 163
MS.gene061197.t1 MTR_3g102600 60.769 130 51 0 11 140 2 131 5.70e-46 160
MS.gene061197.t1 MTR_3g102600 60.377 53 21 0 197 249 131 183 1.34e-14 72.8
MS.gene061197.t1 MTR_4g021855 38.235 238 124 3 17 254 10 224 1.56e-45 163
MS.gene061197.t1 MTR_4g021845 38.627 233 118 5 16 247 9 217 1.30e-44 158
MS.gene061197.t1 MTR_6g045327 35.000 260 136 6 11 261 8 243 7.31e-42 155
MS.gene061197.t1 MTR_4g121020 37.943 282 142 9 96 350 6 281 1.89e-39 152
MS.gene061197.t1 MTR_0450s0040 36.364 242 136 6 21 262 8 231 2.58e-39 147
MS.gene061197.t1 MTR_1g013160 48.344 151 73 2 79 224 1 151 4.53e-36 132
MS.gene061197.t1 MTR_8g105600 35.417 240 150 3 13 251 5 240 7.62e-34 136
MS.gene061197.t1 MTR_5g055260 34.043 235 103 8 17 250 10 193 1.97e-31 122
MS.gene061197.t1 MTR_3g102600 63.415 82 30 0 59 140 1 82 1.26e-30 117
MS.gene061197.t1 MTR_3g102600 60.377 53 21 0 197 249 82 134 2.64e-15 73.6
MS.gene061197.t1 MTR_3g102600 63.415 82 30 0 59 140 1 82 1.54e-30 118
MS.gene061197.t1 MTR_3g102600 42.056 107 45 2 197 303 82 171 2.33e-18 83.6
MS.gene061197.t1 MTR_7g026400 35.096 208 124 3 47 254 17 213 2.95e-30 125
MS.gene061197.t1 MTR_8g032710 30.502 259 153 7 16 250 16 271 3.97e-28 118
MS.gene061197.t1 MTR_3g092780 32.979 188 105 5 21 194 56 236 5.01e-24 107
MS.gene061197.t1 MTR_3g092780 32.979 188 105 5 21 194 56 236 5.32e-24 107
MS.gene061197.t1 MTR_8g093040 28.926 242 134 3 13 254 10 213 1.65e-23 100
MS.gene061197.t1 MTR_3g092780 32.240 183 112 4 21 194 56 235 3.19e-23 104
MS.gene061197.t1 MTR_3g092780 32.240 183 112 4 21 194 56 235 3.20e-23 104
MS.gene061197.t1 MTR_4g023980 31.875 160 85 3 63 221 35 171 2.38e-22 94.7
MS.gene061197.t1 MTR_7g118260 36.090 133 76 2 18 144 11 140 1.24e-21 99.4
MS.gene061197.t1 MTR_6g016850 33.121 157 102 2 13 167 5 160 1.48e-21 92.4
MS.gene061197.t1 MTR_7g118260 36.719 128 72 2 18 139 11 135 2.69e-21 98.2
MS.gene061197.t1 MTR_4g061360 37.705 122 73 1 21 139 89 210 1.72e-19 93.2
MS.gene061197.t1 MTR_4g064730 52.381 84 37 2 174 254 104 187 1.42e-18 87.4
MS.gene061197.t1 MTR_1g067110 34.426 122 77 1 21 139 85 206 2.55e-18 89.4
MS.gene061197.t1 MTR_1g067110 34.426 122 77 1 21 139 85 206 2.79e-18 89.4
MS.gene061197.t1 MTR_3g037390 31.579 133 86 2 13 140 14 146 3.51e-18 88.6
MS.gene061197.t1 MTR_8g024260 33.600 125 80 1 23 144 38 162 1.13e-17 87.0
MS.gene061197.t1 MTR_4g108880 30.303 132 87 2 14 140 24 155 5.92e-17 84.7
MS.gene061197.t1 MTR_7g089010 58.621 58 24 0 197 254 90 147 2.53e-16 80.5
MS.gene061197.t1 MTR_5g014040 33.553 152 84 3 151 293 264 407 1.64e-15 80.1
MS.gene061197.t1 MTR_5g014040 38.400 125 71 3 17 139 14 134 2.48e-15 79.3
MS.gene061197.t1 MTR_4g098870 48.750 80 31 1 191 260 303 382 2.18e-15 79.7
MS.gene061197.t1 MTR_4g098870 34.677 124 77 2 17 139 14 134 6.12e-14 75.1
MS.gene061197.t1 MTR_4g098870 48.750 80 31 1 191 260 323 402 2.30e-15 79.7
MS.gene061197.t1 MTR_4g098870 34.677 124 77 2 17 139 34 154 7.63e-14 74.7
MS.gene061197.t1 MTR_4g098870 48.750 80 31 1 191 260 303 382 2.53e-15 79.3
MS.gene061197.t1 MTR_4g098870 34.677 124 77 2 17 139 14 134 3.94e-14 75.5
MS.gene061197.t1 MTR_5g014040 38.400 125 71 3 17 139 14 134 2.62e-15 79.3
MS.gene061197.t1 MTR_5g014040 32.484 157 84 4 151 293 264 412 1.47e-13 73.9
MS.gene061197.t1 MTR_4g098870 48.750 80 31 1 191 260 342 421 2.69e-15 79.3
MS.gene061197.t1 MTR_4g098870 34.677 124 77 2 17 139 53 173 5.65e-14 75.1
MS.gene061197.t1 MTR_4g098870 48.750 80 31 1 191 260 323 402 2.76e-15 79.3
MS.gene061197.t1 MTR_4g098870 34.677 124 77 2 17 139 34 154 5.52e-14 75.1
MS.gene061197.t1 MTR_7g115530 62.500 56 20 1 196 250 216 271 7.44e-14 73.2
MS.gene061197.t1 MTR_5g014040 32.484 157 84 4 151 293 224 372 1.20e-13 73.9
MS.gene061197.t1 MTR_7g115530 62.500 56 20 1 196 250 216 271 1.33e-13 72.4
MS.gene061197.t1 MTR_7g115530 62.500 56 20 1 196 250 216 271 1.44e-13 72.8
MS.gene061197.t1 MTR_7g115530 62.500 56 20 1 196 250 216 271 2.40e-13 72.8
MS.gene061197.t1 MTR_4g078810 42.857 84 46 1 196 279 156 237 2.86e-13 72.4
MS.gene061197.t1 MTR_2g090675 36.585 123 52 4 134 256 123 219 3.84e-13 72.0
MS.gene061197.t1 MTR_1g080330 60.714 56 21 1 196 250 173 228 4.40e-13 70.5
MS.gene061197.t1 MTR_1g080330 60.714 56 21 1 196 250 173 228 6.38e-13 71.2
MS.gene061197.t1 MTR_6g088950 30.827 133 82 3 47 175 34 160 2.36e-12 67.0
MS.gene061197.t1 MTR_7g115530 61.111 54 20 1 196 248 216 269 4.60e-12 67.4
MS.gene061197.t1 MTR_7g098250 48.000 75 37 2 198 270 262 336 4.99e-12 68.9
MS.gene061197.t1 MTR_7g490310 28.662 157 89 3 13 148 1 155 5.17e-12 66.6
MS.gene061197.t1 MTR_4g081710 49.153 59 29 1 194 251 13 71 5.79e-12 67.8
MS.gene061197.t1 MTR_2g027860 55.000 60 25 2 198 256 49 107 6.25e-12 67.4
MS.gene061197.t1 MTR_6g032990 54.237 59 26 1 194 251 38 96 8.52e-12 67.8
MS.gene061197.t1 MTR_7g115530 60.000 55 21 1 196 249 216 270 1.15e-11 66.2
MS.gene061197.t1 MTR_2g023580 60.000 55 21 1 197 250 54 108 1.57e-11 65.1
MS.gene061197.t1 MTR_5g041350 50.847 59 28 1 194 251 11 69 1.72e-11 66.2
MS.gene061197.t1 MTR_1g093080 44.928 69 36 2 187 254 201 268 2.00e-11 66.2
MS.gene061197.t1 MTR_4g086835 48.276 58 30 0 197 254 273 330 2.26e-11 66.6
MS.gene061197.t1 MTR_2g086450 50.847 59 28 1 194 251 41 99 4.42e-11 65.5
MS.gene061197.t1 MTR_3g450310 49.296 71 29 2 187 250 2 72 5.05e-11 63.2
MS.gene061197.t1 MTR_4g113140 31.250 112 70 3 144 254 187 292 5.53e-11 65.5
MS.gene061197.t1 MTR_0223s0040 54.545 66 29 1 197 261 17 82 7.21e-11 63.5
MS.gene061197.t1 MTR_5g017980 46.552 58 31 0 197 254 191 248 8.73e-11 63.9
MS.gene061197.t1 MTR_5g017980 46.552 58 31 0 197 254 192 249 8.87e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061197.t1 AT2G25180 46.486 370 170 7 17 361 13 379 1.30e-89 288
MS.gene061197.t1 AT4G31920 50.000 318 122 7 17 310 13 317 8.45e-81 264
MS.gene061197.t1 AT1G67710 55.078 256 105 5 16 264 5 257 3.82e-80 261
MS.gene061197.t1 AT3G16857 47.039 304 136 3 8 287 24 326 5.98e-80 265
MS.gene061197.t1 AT5G58080 48.466 326 144 9 7 313 3 323 6.20e-80 264
MS.gene061197.t1 AT3G16857 47.039 304 136 3 8 287 24 326 1.25e-79 265
MS.gene061197.t1 AT2G01760 50.195 257 112 4 17 259 7 261 1.29e-69 229
MS.gene061197.t1 AT2G01760 45.263 285 112 5 17 259 7 289 1.53e-63 214
MS.gene061197.t1 AT3G16857 43.706 286 132 4 56 313 2 286 2.99e-63 219
MS.gene061197.t1 AT5G49240 41.633 245 131 5 11 247 32 272 8.68e-53 182
MS.gene061197.t1 AT1G67710 52.308 195 84 4 76 264 1 192 7.43e-52 184
MS.gene061197.t1 AT2G01760 48.990 198 85 4 76 259 1 196 2.03e-46 166
MS.gene061197.t1 AT3G62670 39.662 237 125 6 22 250 42 268 2.00e-39 149
MS.gene061197.t1 AT1G49190 30.667 300 168 8 17 290 30 315 3.10e-39 149
MS.gene061197.t1 AT1G67710 47.701 174 82 4 97 264 13 183 1.29e-37 145
MS.gene061197.t1 AT5G07210 29.965 287 155 5 21 266 16 297 7.77e-32 130
MS.gene061197.t1 AT5G07210 29.153 295 155 6 21 266 16 305 1.81e-29 123
MS.gene061197.t1 AT2G27070 29.965 287 158 8 21 266 16 300 1.57e-28 120
MS.gene061197.t1 AT1G49190 36.800 125 79 0 17 141 30 154 8.33e-22 100
MS.gene061197.t1 AT2G46790 38.211 123 71 2 21 139 37 158 1.19e-21 98.6
MS.gene061197.t1 AT2G46790 38.211 123 71 2 21 139 37 158 1.35e-21 98.6
MS.gene061197.t1 AT5G24470 36.000 125 75 2 20 140 49 172 1.41e-21 99.0
MS.gene061197.t1 AT5G60100 38.525 122 72 1 21 139 64 185 2.40e-19 90.9
MS.gene061197.t1 AT5G60100 38.525 122 72 1 21 139 64 185 2.40e-19 90.9
MS.gene061197.t1 AT5G60100 38.525 122 72 1 21 139 64 185 2.40e-19 90.9
MS.gene061197.t1 AT1G68210 24.444 270 170 4 21 257 13 281 2.53e-19 92.4
MS.gene061197.t1 AT5G60100 38.525 122 72 1 21 139 64 185 4.49e-19 90.9
MS.gene061197.t1 AT5G60100 38.525 122 72 1 21 139 64 185 4.49e-19 90.9
MS.gene061197.t1 AT5G60100 38.525 122 72 1 21 139 64 185 4.49e-19 90.9
MS.gene061197.t1 AT5G60100 38.525 122 72 1 21 139 64 185 7.40e-19 90.5
MS.gene061197.t1 AT5G05090 50.667 75 37 0 180 254 63 137 1.21e-17 83.6
MS.gene061197.t1 AT5G59570 39.623 106 62 1 151 256 96 199 1.54e-17 84.0
MS.gene061197.t1 AT5G59570 39.623 106 62 1 151 256 96 199 1.54e-17 84.0
MS.gene061197.t1 AT3G46640 58.333 60 25 0 197 256 143 202 2.22e-17 84.0
MS.gene061197.t1 AT3G46640 58.333 60 25 0 197 256 143 202 2.22e-17 84.0
MS.gene061197.t1 AT3G46640 58.333 60 25 0 197 256 143 202 3.45e-17 83.6
MS.gene061197.t1 AT5G02810 34.426 122 77 1 21 139 78 199 3.73e-17 85.5
MS.gene061197.t1 AT2G40970 58.621 58 24 0 197 254 104 161 4.76e-17 81.6
MS.gene061197.t1 AT3G10760 55.385 65 29 0 190 254 97 161 5.78e-17 82.8
MS.gene061197.t1 AT5G61380 31.818 132 85 2 14 140 10 141 8.04e-17 84.3
MS.gene061197.t1 AT5G44190 61.818 55 21 0 196 250 145 199 1.66e-15 78.6
MS.gene061197.t1 AT5G44190 61.818 55 21 0 196 250 145 199 1.75e-15 79.0
MS.gene061197.t1 AT4G18020 34.266 143 85 3 15 150 12 152 1.67e-13 73.6
MS.gene061197.t1 AT4G18020 34.266 143 85 3 15 150 12 152 1.67e-13 73.6
MS.gene061197.t1 AT4G18020 34.266 143 85 3 15 150 12 152 1.67e-13 73.6
MS.gene061197.t1 AT4G18020 34.266 143 85 3 15 150 12 152 1.67e-13 73.6
MS.gene061197.t1 AT4G18020 34.266 143 85 3 15 150 12 152 1.67e-13 73.6
MS.gene061197.t1 AT4G18020 34.266 143 85 3 15 150 12 152 1.67e-13 73.6
MS.gene061197.t1 AT4G18020 34.266 143 85 3 15 150 12 152 2.06e-13 73.6
MS.gene061197.t1 AT4G18020 34.266 143 85 3 15 150 12 152 2.06e-13 73.6
MS.gene061197.t1 AT4G18020 34.266 143 85 3 15 150 12 152 2.06e-13 73.6
MS.gene061197.t1 AT4G18020 34.266 143 85 3 15 150 12 152 2.06e-13 73.6
MS.gene061197.t1 AT4G18020 34.266 143 85 3 15 150 12 152 2.06e-13 73.6
MS.gene061197.t1 AT2G20570 54.098 61 28 0 196 256 151 211 7.63e-13 71.2
MS.gene061197.t1 AT5G45580 50.000 70 32 2 193 261 4 71 1.19e-12 68.9
MS.gene061197.t1 AT5G45580 50.000 70 32 2 193 261 20 87 1.23e-12 68.9
MS.gene061197.t1 AT2G20570 59.259 54 22 0 203 256 174 227 3.05e-12 69.3
MS.gene061197.t1 AT2G01060 55.000 60 25 2 198 256 15 73 5.47e-12 67.4
MS.gene061197.t1 AT3G04030 52.727 55 25 1 198 251 45 99 3.11e-11 65.9
MS.gene061197.t1 AT4G37180 51.667 60 28 1 196 254 208 267 3.14e-11 65.9
MS.gene061197.t1 AT4G37180 51.667 60 28 1 196 254 215 274 3.58e-11 65.5
MS.gene061197.t1 AT3G04030 52.727 55 25 1 198 251 45 99 3.69e-11 65.9
MS.gene061197.t1 AT5G06800 50.000 66 31 2 198 262 192 256 4.94e-11 64.3
MS.gene061197.t1 AT5G18240 54.545 55 24 1 198 251 45 99 5.35e-11 65.1
MS.gene061197.t1 AT5G18240 54.545 55 24 1 198 251 45 99 5.35e-11 65.1
MS.gene061197.t1 AT5G18240 54.545 55 24 1 198 251 45 99 5.79e-11 65.1
MS.gene061197.t1 AT5G18240 54.545 55 24 1 198 251 45 99 5.79e-11 65.1
MS.gene061197.t1 AT5G18240 54.545 55 24 1 198 251 45 99 6.17e-11 65.1

Find 113 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACATTGGTGTCTCGAAAT+TGG 0.196977 8.4:-1981289 None:intergenic
GATTATCTTGCTTCTTCTAA+TGG 0.232806 8.4:+1981846 MS.gene061197:CDS
CCACTTCTGACAGGGTTAAA+AGG 0.242635 8.4:-1981588 None:intergenic
GGACTTAAATTAATGTTATC+AGG 0.249835 8.4:-1981393 None:intergenic
AAGGGTTCAGTAGGTGGATA+TGG 0.252390 8.4:+1981138 MS.gene061197:CDS
AAAAGAAGCCTCGTTTAGTT+TGG 0.279239 8.4:+1980636 MS.gene061197:CDS
CAATGTCAAGATGGCTTACT+TGG 0.323761 8.4:+1981756 MS.gene061197:CDS
TGATTAATGAACTATTAGTT+TGG 0.331944 8.4:-1981899 None:intergenic
GAGCAATTGCTTAATGTAGA+TGG 0.338130 8.4:+1981993 MS.gene061197:CDS
CCCGGCGGGTATGCGTGTTC+TGG 0.339385 8.4:+1978241 MS.gene061197:CDS
CTCACTCAATGGAACAGAAT+TGG 0.343212 8.4:-1981628 None:intergenic
ATCATTAGTGATGGGGTATA+TGG 0.343848 8.4:+1980497 MS.gene061197:CDS
AAAGAAGCCTCGTTTAGTTT+GGG 0.355798 8.4:+1980637 MS.gene061197:CDS
AGACATCGTTGCTGTTCCTT+TGG 0.355803 8.4:+1981722 MS.gene061197:CDS
CATTCCTTCCAAGTTCAAAC+TGG 0.363583 8.4:-1981223 None:intergenic
GATAAATATTCTGCTGAAAC+TGG 0.368030 8.4:-1981368 None:intergenic
CACACCATCCAGTTTGAACT+TGG 0.369083 8.4:+1981215 MS.gene061197:CDS
CTTCTCACATGCAACTATAA+TGG 0.375217 8.4:+1978308 MS.gene061197:CDS
GTTTCTGCAGTTAATCTTGT+CGG 0.381222 8.4:+1980683 MS.gene061197:CDS
TATCAAATGGCTATCTAAAC+AGG 0.381598 8.4:+1981080 MS.gene061197:CDS
CATCGTTGCTGTTCCTTTGG+AGG 0.383670 8.4:+1981725 MS.gene061197:CDS
AACATTGGTGTCTCGAAATT+GGG 0.390536 8.4:-1981288 None:intergenic
ATAAATCACTCTCCTGTTGA+AGG 0.396905 8.4:+1981546 MS.gene061197:CDS
GATTAATGAACTATTAGTTT+GGG 0.399970 8.4:-1981898 None:intergenic
TCAGCAAGTCGGACTTGAAA+TGG 0.400458 8.4:+1980211 MS.gene061197:CDS
TCCAGCACTTTGAGATAAGT+TGG 0.404451 8.4:-1978278 None:intergenic
AAATGTATCCAGCCATTTGC+AGG 0.408981 8.4:+1980880 MS.gene061197:CDS
ACTGCATTTGAATCATCAAT+TGG 0.413674 8.4:-1981414 None:intergenic
TCAAGTTGAGAAGTTCTATT+CGG 0.435476 8.4:+1981932 MS.gene061197:CDS
ATATAATTTGTACCTGCAAA+TGG 0.440246 8.4:-1980892 None:intergenic
TTCACAGCAGAACCTTCAAC+AGG 0.443727 8.4:-1981558 None:intergenic
TGAATTGTTGAGAGAAAAGA+AGG 0.450895 8.4:+1980127 MS.gene061197:CDS
AGCTTGAAGCCATCCATGTC+TGG 0.455651 8.4:-1980185 None:intergenic
GAGAAGTTCTATTCGGATCA+AGG 0.456955 8.4:+1981939 MS.gene061197:CDS
ATTCTAGTTCATGATGGTTG+TGG 0.457111 8.4:+1978178 None:intergenic
ACATGGCATTCAAACGTCAA+TGG 0.462461 8.4:+1981338 MS.gene061197:CDS
AGAATTGGAAGCAAAGTTAG+AGG 0.465879 8.4:-1981613 None:intergenic
AGGGTTCAGTAGGTGGATAT+GGG 0.469935 8.4:+1981139 MS.gene061197:CDS
CATTGCATCGTTTCCTCCAA+AGG 0.471428 8.4:-1981738 None:intergenic
TACTACAACTACGAAGTCAT+TGG 0.471545 8.4:+1980097 MS.gene061197:CDS
GATACAGAATTGATATCAAA+TGG 0.472061 8.4:+1981067 MS.gene061197:CDS
TGACAGTGGCTCTGATGTCT+TGG 0.472341 8.4:-1981455 None:intergenic
CTTGACTTGATGAATGTTGA+AGG 0.474751 8.4:+1980845 MS.gene061197:CDS
TTAAGCTCTTCCATTCGAAT+CGG 0.481435 8.4:-1980416 None:intergenic
CAAGGAATCATGGAATCAAA+TGG 0.482889 8.4:+1981957 MS.gene061197:CDS
TTCAGAATAGTCGTGTGCCT+TGG 0.483059 8.4:+1982021 MS.gene061197:CDS
TTCCTCCTTCAACAATACCT+TGG 0.487555 8.4:+1981824 MS.gene061197:CDS
AATTTGTACCTGCAAATGGC+TGG 0.488638 8.4:-1980888 None:intergenic
TGGAAGAGCTTAAGAGCATA+TGG 0.489491 8.4:+1980426 MS.gene061197:CDS
GTTGGATCCCTACCAGCAAA+AGG 0.495630 8.4:+1981119 MS.gene061197:CDS
CAGCCGACAAAACATGTCAT+TGG 0.496819 8.4:-1981190 None:intergenic
TGAATGTTGAAGGGCTTACA+AGG 0.497287 8.4:+1980855 MS.gene061197:CDS
TTGTTAAAATCCTCACTCAA+TGG 0.497939 8.4:-1981639 None:intergenic
TAGAAAGAAGATTGAAAGCA+AGG 0.500776 8.4:+1980460 MS.gene061197:CDS
TTAGAGGAACCACTTCTGAC+AGG 0.501081 8.4:-1981597 None:intergenic
ATAATCCAAGGTATTGTTGA+AGG 0.508220 8.4:-1981829 None:intergenic
ATCAAGGTATCATTAGTGAT+GGG 0.510346 8.4:+1980489 MS.gene061197:CDS
AATCAAGGTATCATTAGTGA+TGG 0.511362 8.4:+1980488 MS.gene061197:CDS
TGTGTGATTTATAGTGTTGT+CGG 0.513795 8.4:+1980328 MS.gene061197:intron
GAAAGCAAGGATCAAAATCA+AGG 0.515217 8.4:+1980473 MS.gene061197:CDS
CCATCCAGTTTGAACTTGGA+AGG 0.517311 8.4:+1981219 MS.gene061197:CDS
AATGCAGTTACAGATTCCTC+TGG 0.518088 8.4:+1981429 MS.gene061197:CDS
CTTTCACTTTCAGAAAACAT+TGG 0.525109 8.4:-1981303 None:intergenic
TCCAACTTATCTCAAAGTGC+TGG 0.528445 8.4:+1978277 MS.gene061197:CDS
TGGCTATCTAAACAGGATAG+AGG 0.531559 8.4:+1981087 MS.gene061197:CDS
CAATTCTGTTCCATTGAGTG+AGG 0.541688 8.4:+1981629 MS.gene061197:CDS
TGCTAACATCACTAATTACA+AGG 0.543736 8.4:-1980160 None:intergenic
TTCACTTATTGTGTTGTCCA+AGG 0.543954 8.4:-1982038 None:intergenic
CTATTCGGATCAAGGAATCA+TGG 0.544673 8.4:+1981947 MS.gene061197:CDS
CAGCAAAAGGGTTCAGTAGG+TGG 0.548262 8.4:+1981132 MS.gene061197:CDS
GCCAGAACACGCATACCCGC+CGG 0.553676 8.4:-1978242 None:intergenic
ATGGTTGTGGAGGATAGTTT+CGG 0.554649 8.4:+1978191 MS.gene061197:CDS
CCTTCCAAGTTCAAACTGGA+TGG 0.554980 8.4:-1981219 None:intergenic
TTAGGAGAAGGTTTCCCGGC+GGG 0.561505 8.4:+1978227 MS.gene061197:CDS
ATGTCATTGGATGCATATGC+TGG 0.565239 8.4:-1981177 None:intergenic
TAATGGAGACACTAGTTCCA+TGG 0.565315 8.4:+1981863 MS.gene061197:CDS
CCAGAACACGCATACCCGCC+GGG 0.571213 8.4:-1978241 None:intergenic
TTTCAAGTCCGACTTGCTGA+AGG 0.572445 8.4:-1980208 None:intergenic
TTGGATCCCTACCAGCAAAA+GGG 0.576563 8.4:+1981120 MS.gene061197:CDS
TTGACTTGATGAATGTTGAA+GGG 0.580486 8.4:+1980846 MS.gene061197:CDS
GAAACGATGCAATGTCAAGA+TGG 0.582127 8.4:+1981747 MS.gene061197:CDS
GGATAGAGGAGATGATGCGT+TGG 0.582696 8.4:+1981101 MS.gene061197:CDS
TTGGGCTAAACTTTGTACCA+TGG 0.585108 8.4:-1981880 None:intergenic
AAGAAAGGTACAAAGTGAGG+AGG 0.585901 8.4:+1980574 MS.gene061197:CDS
GAAAGTTCGAGTTCTTTACA+TGG 0.585974 8.4:+1981321 MS.gene061197:CDS
TTCAAGCTCCTTCAGCAAGT+CGG 0.589889 8.4:+1980200 MS.gene061197:CDS
AACATTTGCATTATTGACAG+TGG 0.591551 8.4:-1981469 None:intergenic
GGGTTCAGTAGGTGGATATG+GGG 0.592030 8.4:+1981140 MS.gene061197:CDS
CTAGTTCATGATGGTTGTGG+AGG 0.595087 8.4:+1978181 None:intergenic
AGAAGAAGCAAGATAATCCA+AGG 0.597269 8.4:-1981841 None:intergenic
TGATGTTAGCATGCCAGACA+TGG 0.598210 8.4:+1980172 MS.gene061197:CDS
TCTAGTGAAACCGATTCGAA+TGG 0.598674 8.4:+1980406 MS.gene061197:CDS
ATGTTATGAGACTGAAGTGG+AGG 0.606395 8.4:-1981264 None:intergenic
GAATAGAACTTCTCAACTTG+AGG 0.607973 8.4:-1981930 None:intergenic
GCTAACAAGAATAAAACGCA+TGG 0.616521 8.4:+1980545 MS.gene061197:CDS
GGTAAATGATGATATAGAGA+AGG 0.617327 8.4:+1980349 MS.gene061197:CDS
AATGTCAAGATGGCTTACTT+GGG 0.618703 8.4:+1981757 MS.gene061197:CDS
TACCAGCAAAAGGGTTCAGT+AGG 0.620378 8.4:+1981129 MS.gene061197:CDS
TAGAGGAACCACTTCTGACA+GGG 0.620617 8.4:-1981596 None:intergenic
ATCCAAGGTATTGTTGAAGG+AGG 0.626426 8.4:-1981826 None:intergenic
GTCACATAAACTCACTGTCA+AGG 0.629956 8.4:-1980706 None:intergenic
TCAAGGTATCATTAGTGATG+GGG 0.639460 8.4:+1980490 MS.gene061197:CDS
TGTGTGAGCTTACTGATGAA+AGG 0.640199 8.4:-1980239 None:intergenic
TCTGATGTCTTGGAAGCCAG+AGG 0.643398 8.4:-1981445 None:intergenic
CTTAAATTAATGTTATCAGG+CGG 0.647690 8.4:-1981390 None:intergenic
GTTAGCATGCCAGACATGGA+TGG 0.657571 8.4:+1980176 MS.gene061197:CDS
TGAGCTTACTGATGAAAGGT+AGG 0.657693 8.4:-1980235 None:intergenic
GTGATGAAGAGTGTGATACA+TGG 0.658673 8.4:+1980371 MS.gene061197:CDS
TGTTATGAGACTGAAGTGGA+GGG 0.664363 8.4:-1981263 None:intergenic
GGAATGTTATGAGACTGAAG+TGG 0.672395 8.4:-1981267 None:intergenic
GAATGTTGAAGGGCTTACAA+GGG 0.675399 8.4:+1980856 MS.gene061197:CDS
TCATGATCCCAAACTAAACG+AGG 0.706682 8.4:-1980644 None:intergenic
TTTAGGAGAAGGTTTCCCGG+CGG 0.790759 8.4:+1978226 MS.gene061197:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAAAATCTAAAACAAAA+GGG + chr8.4:1979805-1979824 MS.gene061197:intron 10.0%
!!! TTAAAAAAATCTAAAACAAA+AGG + chr8.4:1979804-1979823 MS.gene061197:intron 10.0%
!!! TTTTTATGATAGTAAATAAA+TGG - chr8.4:1979573-1979592 None:intergenic 10.0%
!!! TTTTTTTCTTTTTTTTTTTG+AGG - chr8.4:1979362-1979381 None:intergenic 10.0%
!! AAATAAATGGAAATTATCTT+CGG - chr8.4:1979560-1979579 None:intergenic 15.0%
!! CAATTTACTAAAAAAAACTA+AGG - chr8.4:1978490-1978509 None:intergenic 15.0%
!!! CTTAGTTTTTTTTAGTAAAT+TGG + chr8.4:1978488-1978507 MS.gene061197:intron 15.0%
!! AAATATCCGAATATAAGAAA+TGG - chr8.4:1980304-1980323 None:intergenic 20.0%
!! AATGATTATTACTGCTTTAT+TGG + chr8.4:1979871-1979890 MS.gene061197:intron 20.0%
!! ATAAGAAATGGATCTTAATT+AGG - chr8.4:1980292-1980311 None:intergenic 20.0%
!! GAAGAGACATTTAAATAAAA+AGG - chr8.4:1980037-1980056 None:intergenic 20.0%
!! TAAGATCCATTTCTTATATT+CGG + chr8.4:1980295-1980314 MS.gene061197:intron 20.0%
!! TGATGAATGATTCTTAAATA+TGG + chr8.4:1980758-1980777 MS.gene061197:intron 20.0%
!! TGATTAATGAACTATTAGTT+TGG - chr8.4:1981902-1981921 None:intergenic 20.0%
!!! AAACCTTTTTTTTTTCTTTG+AGG + chr8.4:1978449-1978468 MS.gene061197:intron 20.0%
!!! AGATGTGTTACAATTTTTTA+GGG - chr8.4:1979002-1979021 None:intergenic 20.0%
!!! ATGATTATTACTGCTTTATT+GGG + chr8.4:1979872-1979891 MS.gene061197:intron 20.0%
!!! ATTCTTGTTAGCAATATTTT+CGG - chr8.4:1980537-1980556 None:intergenic 20.0%
!!! GATTAATGAACTATTAGTTT+GGG - chr8.4:1981901-1981920 None:intergenic 20.0%
!!! TTTGAGATTTTTTGACAAAA+GGG - chr8.4:1980926-1980945 None:intergenic 20.0%
!!! TTTTTAGTAAATTGGTGATT+AGG + chr8.4:1978496-1978515 MS.gene061197:intron 20.0%
! AAAAAAATCACTAACATGTC+AGG - chr8.4:1979898-1979917 None:intergenic 25.0%
! AACTACAACCTATTTATCAT+AGG + chr8.4:1979211-1979230 MS.gene061197:intron 25.0%
! AAGTTACTCAATAGTTATAG+TGG - chr8.4:1978739-1978758 None:intergenic 25.0%
! ACACCAATAAAACTTATTTG+TGG + chr8.4:1978562-1978581 MS.gene061197:intron 25.0%
! AGTGTATATTTATGCTTCAT+AGG + chr8.4:1979301-1979320 MS.gene061197:intron 25.0%
! AGTTGAAGAAACTACATATT+AGG - chr8.4:1980795-1980814 None:intergenic 25.0%
! ATTGAATGAACGAAAAAGAA+AGG - chr8.4:1978341-1978360 None:intergenic 25.0%
! CTTAAATTAATGTTATCAGG+CGG - chr8.4:1981393-1981412 None:intergenic 25.0%
! GATACAGAATTGATATCAAA+TGG + chr8.4:1981067-1981086 MS.gene061197:CDS 25.0%
! GGACTTAAATTAATGTTATC+AGG - chr8.4:1981396-1981415 None:intergenic 25.0%
! GTTCTAGTATGATTTAAACA+TGG - chr8.4:1979188-1979207 None:intergenic 25.0%
! TTACATAGCCTATGATAAAT+AGG - chr8.4:1979222-1979241 None:intergenic 25.0%
!! AAAAATTTGTTTGTTCCGTT+CGG + chr8.4:1978671-1978690 MS.gene061197:intron 25.0%
!! AATTTTCATATGAGAATTGC+TGG + chr8.4:1979987-1980006 MS.gene061197:intron 25.0%
!! ATATAATTTGTACCTGCAAA+TGG - chr8.4:1980895-1980914 None:intergenic 25.0%
!! ATCAAGTCAAGTATTTTCTT+AGG - chr8.4:1980836-1980855 None:intergenic 25.0%
!! GGTCTTTTATATAATAATGC+TGG + chr8.4:1980008-1980027 MS.gene061197:intron 25.0%
!! TTTTTCCTTCTTTTTCTGAA+TGG + chr8.4:1979917-1979936 MS.gene061197:intron 25.0%
!!! CAAAACAGATTTTAAGAGAA+AGG - chr8.4:1980265-1980284 None:intergenic 25.0%
!!! GAGATGTGTTACAATTTTTT+AGG - chr8.4:1979003-1979022 None:intergenic 25.0%
!!! GTTTGAGATTTTTTGACAAA+AGG - chr8.4:1980927-1980946 None:intergenic 25.0%
!!! TTGAATAACTTTTCGATTTG+AGG + chr8.4:1979670-1979689 MS.gene061197:intron 25.0%
AACATTTGCATTATTGACAG+TGG - chr8.4:1981472-1981491 None:intergenic 30.0%
ACGCCTCAAAGAAAAAAAAA+AGG - chr8.4:1978455-1978474 None:intergenic 30.0%
ACTCAATAGTTATAGTGGTA+CGG - chr8.4:1978734-1978753 None:intergenic 30.0%
ACTGCATTTGAATCATCAAT+TGG - chr8.4:1981417-1981436 None:intergenic 30.0%
ATAGGCTATGTAAATGTATC+CGG + chr8.4:1979229-1979248 MS.gene061197:intron 30.0%
ATCCTAATAGTGTTTCTCTA+AGG + chr8.4:1978820-1978839 MS.gene061197:intron 30.0%
ATGTAGAGAGTAGAAAAACA+AGG - chr8.4:1978932-1978951 None:intergenic 30.0%
CTTTCACTTTCAGAAAACAT+TGG - chr8.4:1981306-1981325 None:intergenic 30.0%
CTTTGGCAAAAGAATGAATT+AGG - chr8.4:1978768-1978787 None:intergenic 30.0%
GATAAATATTCTGCTGAAAC+TGG - chr8.4:1981371-1981390 None:intergenic 30.0%
GATTATCTTGCTTCTTCTAA+TGG + chr8.4:1981846-1981865 MS.gene061197:CDS 30.0%
GGTAAATGATGATATAGAGA+AGG + chr8.4:1980349-1980368 MS.gene061197:CDS 30.0%
TAAACCTTGTATCAGATGAA+AGG - chr8.4:1978961-1978980 None:intergenic 30.0%
TAGAAACCACAACAAAACTA+GGG - chr8.4:1978906-1978925 None:intergenic 30.0%
TAGAAAGAAGATTGAAAGCA+AGG + chr8.4:1980460-1980479 MS.gene061197:CDS 30.0%
TATCAAATGGCTATCTAAAC+AGG + chr8.4:1981080-1981099 MS.gene061197:CDS 30.0%
TCAAAACCACACCTAAAATT+GGG + chr8.4:1979115-1979134 MS.gene061197:intron 30.0%
TGAATTGTTGAGAGAAAAGA+AGG + chr8.4:1980127-1980146 MS.gene061197:CDS 30.0%
TGATTTAAACATGGTGTTTC+TGG - chr8.4:1979179-1979198 None:intergenic 30.0%
TGCAATCGAAAATGAAAATG+AGG - chr8.4:1981045-1981064 None:intergenic 30.0%
TGCTAACATCACTAATTACA+AGG - chr8.4:1980163-1980182 None:intergenic 30.0%
TGTAGAGAGTAGAAAAACAA+GGG - chr8.4:1978931-1978950 None:intergenic 30.0%
TGTGTGATTTATAGTGTTGT+CGG + chr8.4:1980328-1980347 MS.gene061197:intron 30.0%
TTAGAAACCACAACAAAACT+AGG - chr8.4:1978907-1978926 None:intergenic 30.0%
TTCTTCAACTATTATTGCAG+AGG + chr8.4:1980805-1980824 MS.gene061197:intron 30.0%
TTGTTAAAATCCTCACTCAA+TGG - chr8.4:1981642-1981661 None:intergenic 30.0%
TTTCAACCGTATTTGCAAAA+AGG - chr8.4:1980975-1980994 None:intergenic 30.0%
! AAAAAGAAAGGTACAAAGTG+AGG + chr8.4:1980571-1980590 MS.gene061197:CDS 30.0%
! AATCAAGGTATCATTAGTGA+TGG + chr8.4:1980488-1980507 MS.gene061197:CDS 30.0%
! ATCAAGGTATCATTAGTGAT+GGG + chr8.4:1980489-1980508 MS.gene061197:CDS 30.0%
! TAAATCACTAGCTTGATTCT+TGG + chr8.4:1980059-1980078 MS.gene061197:intron 30.0%
! TTGACTTGATGAATGTTGAA+GGG + chr8.4:1980846-1980865 MS.gene061197:CDS 30.0%
! TTTTGATGTTGTGAGTAACA+TGG - chr8.4:1981798-1981817 None:intergenic 30.0%
! TTTTGCCATTCAGAAAAAGA+AGG - chr8.4:1979925-1979944 None:intergenic 30.0%
!! ATAATCCAAGGTATTGTTGA+AGG - chr8.4:1981832-1981851 None:intergenic 30.0%
!! GCTCCACAAATAAGTTTTAT+TGG - chr8.4:1978568-1978587 None:intergenic 30.0%
!! TCAAGTTGAGAAGTTCTATT+CGG + chr8.4:1981932-1981951 MS.gene061197:CDS 30.0%
!! TTGGAAGTAGCTTTGATATA+AGG + chr8.4:1978426-1978445 MS.gene061197:intron 30.0%
AAAAGAAGCCTCGTTTAGTT+TGG + chr8.4:1980636-1980655 MS.gene061197:CDS 35.0%
AACGCATGGACAAAAAAGAA+AGG + chr8.4:1980559-1980578 MS.gene061197:CDS 35.0%
AATGTCAAGATGGCTTACTT+GGG + chr8.4:1981757-1981776 MS.gene061197:CDS 35.0%
ACTATTAGGATTGAGTCTCT+TGG - chr8.4:1978811-1978830 None:intergenic 35.0%
AGAAGAAGCAAGATAATCCA+AGG - chr8.4:1981844-1981863 None:intergenic 35.0%
AGAATTGGAAGCAAAGTTAG+AGG - chr8.4:1981616-1981635 None:intergenic 35.0%
ATAAATCACTCTCCTGTTGA+AGG + chr8.4:1981546-1981565 MS.gene061197:CDS 35.0%
CAAGGAATCATGGAATCAAA+TGG + chr8.4:1981957-1981976 MS.gene061197:CDS 35.0%
CACCTTAGAGAAACACTATT+AGG - chr8.4:1978825-1978844 None:intergenic 35.0%
CACTAATACATATCAGACTC+CGG - chr8.4:1979251-1979270 None:intergenic 35.0%
CTCAAAACCACACCTAAAAT+TGG + chr8.4:1979114-1979133 MS.gene061197:intron 35.0%
CTTCTCACATGCAACTATAA+TGG + chr8.4:1978308-1978327 MS.gene061197:CDS 35.0%
GAAAGCAAGGATCAAAATCA+AGG + chr8.4:1980473-1980492 MS.gene061197:CDS 35.0%
GAATAGAACTTCTCAACTTG+AGG - chr8.4:1981933-1981952 None:intergenic 35.0%
GAGCAATTGCTTAATGTAGA+TGG + chr8.4:1981993-1982012 MS.gene061197:CDS 35.0%
GCAACGGTGTAAACATATAT+TGG + chr8.4:1978407-1978426 MS.gene061197:intron 35.0%
GCTAACAAGAATAAAACGCA+TGG + chr8.4:1980545-1980564 MS.gene061197:CDS 35.0%
GTCGAAGAAAAATGAGAACT+GGG - chr8.4:1981702-1981721 None:intergenic 35.0%
GTTTCTGCAGTTAATCTTGT+CGG + chr8.4:1980683-1980702 MS.gene061197:CDS 35.0%
TACTACAACTACGAAGTCAT+TGG + chr8.4:1980097-1980116 MS.gene061197:CDS 35.0%
TCTCTTTGCAACAAACACTA+GGG - chr8.4:1978871-1978890 None:intergenic 35.0%
TGTCGAAGAAAAATGAGAAC+TGG - chr8.4:1981703-1981722 None:intergenic 35.0%
TTAAGCTCTTCCATTCGAAT+CGG - chr8.4:1980419-1980438 None:intergenic 35.0%
TTCTCTTTGCAACAAACACT+AGG - chr8.4:1978872-1978891 None:intergenic 35.0%
! AAACATTGGTGTCTCGAAAT+TGG - chr8.4:1981292-1981311 None:intergenic 35.0%
! AAAGAAGCCTCGTTTAGTTT+GGG + chr8.4:1980637-1980656 MS.gene061197:CDS 35.0%
! AACATTGGTGTCTCGAAATT+GGG - chr8.4:1981291-1981310 None:intergenic 35.0%
! ATCATTAGTGATGGGGTATA+TGG + chr8.4:1980497-1980516 MS.gene061197:CDS 35.0%
! CATCCAATGACATGTTTTGT+CGG + chr8.4:1981187-1981206 MS.gene061197:CDS 35.0%
! CTTGACTTGATGAATGTTGA+AGG + chr8.4:1980845-1980864 MS.gene061197:CDS 35.0%
! GAAAGTTCGAGTTCTTTACA+TGG + chr8.4:1981321-1981340 MS.gene061197:CDS 35.0%
! GTTAGCAATATTTTCGGATG+AGG - chr8.4:1980531-1980550 None:intergenic 35.0%
! TCAAGGTATCATTAGTGATG+GGG + chr8.4:1980490-1980509 MS.gene061197:CDS 35.0%
! TCACCTCCTTTTTGCAAATA+CGG + chr8.4:1980966-1980985 MS.gene061197:intron 35.0%
! TTTGAGGTAGCTTAGACATT+CGG - chr8.4:1979346-1979365 None:intergenic 35.0%
!!! GGTGATTAGGTTAGTTTTCA+CGG + chr8.4:1978509-1978528 MS.gene061197:intron 35.0%
!!! GTTAGTTTTCACGGTTTGAA+CGG + chr8.4:1978518-1978537 MS.gene061197:intron 35.0%
!!! TACAAACCCTAGTTTTGTTG+TGG + chr8.4:1978897-1978916 MS.gene061197:intron 35.0%
AAATGTATCCAGCCATTTGC+AGG + chr8.4:1980880-1980899 MS.gene061197:CDS 40.0%
AATGCAGTTACAGATTCCTC+TGG + chr8.4:1981429-1981448 MS.gene061197:CDS 40.0%
AATTTGTACCTGCAAATGGC+TGG - chr8.4:1980891-1980910 None:intergenic 40.0%
ACATGGCATTCAAACGTCAA+TGG + chr8.4:1981338-1981357 MS.gene061197:CDS 40.0%
ACTATTCTCCTCTCATGCAA+CGG + chr8.4:1978391-1978410 MS.gene061197:intron 40.0%
ATCAAGCAACCAAAACAACG+TGG + chr8.4:1978594-1978613 MS.gene061197:intron 40.0%
ATGGTTGTGGAGGATAGTTT+CGG + chr8.4:1978191-1978210 MS.gene061197:CDS 40.0%
ATGTCATTGGATGCATATGC+TGG - chr8.4:1981180-1981199 None:intergenic 40.0%
ATGTTATGAGACTGAAGTGG+AGG - chr8.4:1981267-1981286 None:intergenic 40.0%
CAACCGTATTTGCAAAAAGG+AGG - chr8.4:1980972-1980991 None:intergenic 40.0%
CAACTGATATGTGTGTGAGT+AGG - chr8.4:1979400-1979419 None:intergenic 40.0%
CAATGTCAAGATGGCTTACT+TGG + chr8.4:1981756-1981775 MS.gene061197:CDS 40.0%
CAATTCTGTTCCATTGAGTG+AGG + chr8.4:1981629-1981648 MS.gene061197:CDS 40.0%
CATTCCTTCCAAGTTCAAAC+TGG - chr8.4:1981226-1981245 None:intergenic 40.0%
CTATTCGGATCAAGGAATCA+TGG + chr8.4:1981947-1981966 MS.gene061197:CDS 40.0%
CTCACCTTTCATCTGATACA+AGG + chr8.4:1978954-1978973 MS.gene061197:intron 40.0%
CTCACTCAATGGAACAGAAT+TGG - chr8.4:1981631-1981650 None:intergenic 40.0%
GAAACGATGCAATGTCAAGA+TGG + chr8.4:1981747-1981766 MS.gene061197:CDS 40.0%
GAATGTTGAAGGGCTTACAA+GGG + chr8.4:1980856-1980875 MS.gene061197:CDS 40.0%
GAGAAGTTCTATTCGGATCA+AGG + chr8.4:1981939-1981958 MS.gene061197:CDS 40.0%
GGAATGTTATGAGACTGAAG+TGG - chr8.4:1981270-1981289 None:intergenic 40.0%
GTCACATAAACTCACTGTCA+AGG - chr8.4:1980709-1980728 None:intergenic 40.0%
TAATGGAGACACTAGTTCCA+TGG + chr8.4:1981863-1981882 MS.gene061197:CDS 40.0%
TCAAGCAACCAAAACAACGT+GGG + chr8.4:1978595-1978614 MS.gene061197:intron 40.0%
TCATGATCCCAAACTAAACG+AGG - chr8.4:1980647-1980666 None:intergenic 40.0%
TCCAACTTATCTCAAAGTGC+TGG + chr8.4:1978277-1978296 MS.gene061197:CDS 40.0%
TGAATGTTGAAGGGCTTACA+AGG + chr8.4:1980855-1980874 MS.gene061197:CDS 40.0%
TGAGCTTACTGATGAAAGGT+AGG - chr8.4:1980238-1980257 None:intergenic 40.0%
TGGCTATCTAAACAGGATAG+AGG + chr8.4:1981087-1981106 MS.gene061197:CDS 40.0%
TGTGTGAGCTTACTGATGAA+AGG - chr8.4:1980242-1980261 None:intergenic 40.0%
TGTTATGAGACTGAAGTGGA+GGG - chr8.4:1981266-1981285 None:intergenic 40.0%
TTCCTCCTTCAACAATACCT+TGG + chr8.4:1981824-1981843 MS.gene061197:CDS 40.0%
TTGAGGTAGCTTAGACATTC+GGG - chr8.4:1979345-1979364 None:intergenic 40.0%
TTGCAACAAACACTAGGGTT+GGG - chr8.4:1978866-1978885 None:intergenic 40.0%
TTGGGCTAAACTTTGTACCA+TGG - chr8.4:1981883-1981902 None:intergenic 40.0%
TTTGCAACAAACACTAGGGT+TGG - chr8.4:1978867-1978886 None:intergenic 40.0%
! AAGAAAGGTACAAAGTGAGG+AGG + chr8.4:1980574-1980593 MS.gene061197:CDS 40.0%
! ACTAAACGAGGCTTCTTTTG+AGG - chr8.4:1980635-1980654 None:intergenic 40.0%
! AGACTCCGATTTTCTCACTT+CGG + chr8.4:1979066-1979085 MS.gene061197:intron 40.0%
! GTGATGAAGAGTGTGATACA+TGG + chr8.4:1980371-1980390 MS.gene061197:CDS 40.0%
! TCTTTTGCCAAGACTGTTGT+GGG + chr8.4:1979453-1979472 MS.gene061197:intron 40.0%
! TGGAAGAGCTTAAGAGCATA+TGG + chr8.4:1980426-1980445 MS.gene061197:CDS 40.0%
! TGTTGTGGGTGTGTTTGTTT+TGG + chr8.4:1979467-1979486 MS.gene061197:intron 40.0%
! TTCTTTTGCCAAGACTGTTG+TGG + chr8.4:1979452-1979471 MS.gene061197:intron 40.0%
!! ATCCAAGGTATTGTTGAAGG+AGG - chr8.4:1981829-1981848 None:intergenic 40.0%
!! TCCAGCACTTTGAGATAAGT+TGG - chr8.4:1978281-1978300 None:intergenic 40.0%
!! TCTAGTGAAACCGATTCGAA+TGG + chr8.4:1980406-1980425 MS.gene061197:CDS 40.0%
!!! GTTGACGATGTTTTAGGAGA+AGG + chr8.4:1978215-1978234 MS.gene061197:CDS 40.0%
!!! TGTTTTAGGAGAAGGTTTCC+CGG + chr8.4:1978223-1978242 MS.gene061197:CDS 40.0%
!!! TTCGGTGTTGACGATGTTTT+AGG + chr8.4:1978209-1978228 MS.gene061197:CDS 40.0%
AAACACACCCACAACAGTCT+TGG - chr8.4:1979463-1979482 None:intergenic 45.0%
AAGGGTTCAGTAGGTGGATA+TGG + chr8.4:1981138-1981157 MS.gene061197:CDS 45.0%
AGGATTGAGTCTCTTGGTCA+CGG - chr8.4:1978805-1978824 None:intergenic 45.0%
AGGGTTCAGTAGGTGGATAT+GGG + chr8.4:1981139-1981158 MS.gene061197:CDS 45.0%
CACACCATCCAGTTTGAACT+TGG + chr8.4:1981215-1981234 MS.gene061197:CDS 45.0%
CAGCCGACAAAACATGTCAT+TGG - chr8.4:1981193-1981212 None:intergenic 45.0%
CATTGCATCGTTTCCTCCAA+AGG - chr8.4:1981741-1981760 None:intergenic 45.0%
CCAATACTGACCTACTTTGG+AGG + chr8.4:1978695-1978714 MS.gene061197:intron 45.0%
CCACTTCTGACAGGGTTAAA+AGG - chr8.4:1981591-1981610 None:intergenic 45.0%
CCATCCAGTTTGAACTTGGA+AGG + chr8.4:1981219-1981238 MS.gene061197:CDS 45.0%
CCTCCAAAGTAGGTCAGTAT+TGG - chr8.4:1978698-1978717 None:intergenic 45.0%
CCTTCCAAGTTCAAACTGGA+TGG - chr8.4:1981222-1981241 None:intergenic 45.0%
CGGTTTGAACGGTTGTGAAT+CGG + chr8.4:1978529-1978548 MS.gene061197:intron 45.0%
CTTGGTCACGGAAAGAGATT+CGG - chr8.4:1978793-1978812 None:intergenic 45.0%
GAAGTGAGAAAATCGGAGTC+TGG - chr8.4:1979067-1979086 None:intergenic 45.0%
GGTCCAATACTGACCTACTT+TGG + chr8.4:1978692-1978711 MS.gene061197:intron 45.0%
TACCAGCAAAAGGGTTCAGT+AGG + chr8.4:1981129-1981148 MS.gene061197:CDS 45.0%
TAGAGGAACCACTTCTGACA+GGG - chr8.4:1981599-1981618 None:intergenic 45.0%
TCAGCAAGTCGGACTTGAAA+TGG + chr8.4:1980211-1980230 MS.gene061197:CDS 45.0%
TGATGTTAGCATGCCAGACA+TGG + chr8.4:1980172-1980191 MS.gene061197:CDS 45.0%
TGTTTACACCGTTGCATGAG+AGG - chr8.4:1978402-1978421 None:intergenic 45.0%
TTAGAGGAACCACTTCTGAC+AGG - chr8.4:1981600-1981619 None:intergenic 45.0%
TTCAAGCTCCTTCAGCAAGT+CGG + chr8.4:1980200-1980219 MS.gene061197:CDS 45.0%
TTCACAGCAGAACCTTCAAC+AGG - chr8.4:1981561-1981580 None:intergenic 45.0%
TTCAGAATAGTCGTGTGCCT+TGG + chr8.4:1982021-1982040 MS.gene061197:CDS 45.0%
TTGGATCCCTACCAGCAAAA+GGG + chr8.4:1981120-1981139 MS.gene061197:CDS 45.0%
TTGGTCACGGAAAGAGATTC+GGG - chr8.4:1978792-1978811 None:intergenic 45.0%
! ACTGAACCCTTTTGCTGGTA+GGG - chr8.4:1981129-1981148 None:intergenic 45.0%
! AGACATCGTTGCTGTTCCTT+TGG + chr8.4:1981722-1981741 MS.gene061197:CDS 45.0%
! AGCAATCGAGGCCCAATTTT+AGG - chr8.4:1979129-1979148 None:intergenic 45.0%
! CCTTTTAACCCTGTCAGAAG+TGG + chr8.4:1981588-1981607 MS.gene061197:CDS 45.0%
! GACTCCGATTTTCTCACTTC+GGG + chr8.4:1979067-1979086 MS.gene061197:intron 45.0%
! GTGTGAGTAGGTAGCTTTAC+TGG - chr8.4:1979388-1979407 None:intergenic 45.0%
! TACTGAACCCTTTTGCTGGT+AGG - chr8.4:1981130-1981149 None:intergenic 45.0%
! TAGGTCAGTATTGGACCGAA+CGG - chr8.4:1978689-1978708 None:intergenic 45.0%
! TCTGGAACTGCCAAAATCGA+CGG - chr8.4:1979161-1979180 None:intergenic 45.0%
!! TTTCAAGTCCGACTTGCTGA+AGG - chr8.4:1980211-1980230 None:intergenic 45.0%
!!! AAAATATTTTGTTAAAAAAA+TGG - chr8.4:1979708-1979727 None:intergenic 5.0%
!!! AAATTATATAAGTATTTTTT+TGG + chr8.4:1979755-1979774 MS.gene061197:intron 5.0%
AGCAACCAAAACAACGTGGG+TGG + chr8.4:1978598-1978617 MS.gene061197:intron 50.0%
AGCTTGAAGCCATCCATGTC+TGG - chr8.4:1980188-1980207 None:intergenic 50.0%
CAGCAAAAGGGTTCAGTAGG+TGG + chr8.4:1981132-1981151 MS.gene061197:CDS 50.0%
CGGAAAGAGATTCGGGACTT+TGG - chr8.4:1978785-1978804 None:intergenic 50.0%
GGATAGAGGAGATGATGCGT+TGG + chr8.4:1981101-1981120 MS.gene061197:CDS 50.0%
GGGTTCAGTAGGTGGATATG+GGG + chr8.4:1981140-1981159 MS.gene061197:CDS 50.0%
GTTAGCATGCCAGACATGGA+TGG + chr8.4:1980176-1980195 MS.gene061197:CDS 50.0%
GTTGGATCCCTACCAGCAAA+AGG + chr8.4:1981119-1981138 MS.gene061197:CDS 50.0%
TCTGATGTCTTGGAAGCCAG+AGG - chr8.4:1981448-1981467 None:intergenic 50.0%
TGCGCCCGAAGTGAGAAAAT+CGG - chr8.4:1979074-1979093 None:intergenic 50.0%
TTTAGGAGAAGGTTTCCCGG+CGG + chr8.4:1978226-1978245 MS.gene061197:CDS 50.0%
! CACCTACTGAACCCTTTTGC+TGG - chr8.4:1981134-1981153 None:intergenic 50.0%
! CATCGTTGCTGTTCCTTTGG+AGG + chr8.4:1981725-1981744 MS.gene061197:CDS 50.0%
!! TCGAGGCCCAATTTTAGGTG+TGG - chr8.4:1979124-1979143 None:intergenic 50.0%
!! TGACAGTGGCTCTGATGTCT+TGG - chr8.4:1981458-1981477 None:intergenic 50.0%
!!! TCCAAATCGGCCGTCGATTT+TGG + chr8.4:1979148-1979167 MS.gene061197:intron 50.0%
GCCAAAATCGACGGCCGATT+TGG - chr8.4:1979152-1979171 None:intergenic 55.0%
GCTGCTCTCTCCTCCAAAGT+AGG - chr8.4:1978708-1978727 None:intergenic 55.0%
GGGCCTCGATTGCTCCAAAT+CGG + chr8.4:1979135-1979154 MS.gene061197:intron 55.0%
TTAGGAGAAGGTTTCCCGGC+GGG + chr8.4:1978227-1978246 MS.gene061197:CDS 55.0%
! AGCTGCCACCCACGTTGTTT+TGG - chr8.4:1978606-1978625 None:intergenic 55.0%
!! CGGCCGATTTGGAGCAATCG+AGG - chr8.4:1979141-1979160 None:intergenic 60.0%
CCAGAACACGCATACCCGCC+GGG - chr8.4:1978244-1978263 None:intergenic 65.0%
GCCAGAACACGCATACCCGC+CGG - chr8.4:1978245-1978264 None:intergenic 65.0%
CCCGGCGGGTATGCGTGTTC+TGG + chr8.4:1978241-1978260 MS.gene061197:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 1978191 1982055 1978191 ID=MS.gene061197
chr8.4 mRNA 1978191 1982055 1978191 ID=MS.gene061197.t1;Parent=MS.gene061197
chr8.4 exon 1978191 1978329 1978191 ID=MS.gene061197.t1.exon1;Parent=MS.gene061197.t1
chr8.4 CDS 1978191 1978329 1978191 ID=cds.MS.gene061197.t1;Parent=MS.gene061197.t1
chr8.4 exon 1980096 1980248 1980096 ID=MS.gene061197.t1.exon2;Parent=MS.gene061197.t1
chr8.4 CDS 1980096 1980248 1980096 ID=cds.MS.gene061197.t1;Parent=MS.gene061197.t1
chr8.4 exon 1980342 1980713 1980342 ID=MS.gene061197.t1.exon3;Parent=MS.gene061197.t1
chr8.4 CDS 1980342 1980713 1980342 ID=cds.MS.gene061197.t1;Parent=MS.gene061197.t1
chr8.4 exon 1980825 1980901 1980825 ID=MS.gene061197.t1.exon4;Parent=MS.gene061197.t1
chr8.4 CDS 1980825 1980901 1980825 ID=cds.MS.gene061197.t1;Parent=MS.gene061197.t1
chr8.4 exon 1981066 1982055 1981066 ID=MS.gene061197.t1.exon5;Parent=MS.gene061197.t1
chr8.4 CDS 1981066 1982055 1981066 ID=cds.MS.gene061197.t1;Parent=MS.gene061197.t1
Gene Sequence

>MS.gene061197

ATGGTTGTGGAGGATAGTTTCGGTGTTGACGATGTTTTAGGAGAAGGTTTCCCGGCGGGTATGCGTGTTCTGGCAGTTGATGACGATCCAACTTATCTCAAAGTGCTGGAGAAGCAACTTCTCACATGCAACTATAATGGTTCGTTGCCTTTCTTTTTCGTTCATTCAATATGATTACGCATTGATTATTCTTTATGTTTACTATTCTCCTCTCATGCAACGGTGTAAACATATATTGGAAGTAGCTTTGATATAAGGAAACCTTTTTTTTTTCTTTGAGGCGTTGATATAGTAAACCTTAGTTTTTTTTAGTAAATTGGTGATTAGGTTAGTTTTCACGGTTTGAACGGTTGTGAATCGGTTAAAAAATCACACCAATAAAACTTATTTGTGGAGCAAATGAATCAAGCAACCAAAACAACGTGGGTGGCAGCTCTAAACACAATCAAAAGATAGAACAACAAAAGAGAAAGAACACACAAAAATTTGTTTGTTCCGTTCGGTCCAATACTGACCTACTTTGGAGGAGAGAGCAGCTCTCCGTACCACTATAACTATTGAGTAACTTTACAAACCTAATTCATTCTTTTGCCAAAGTCCCGAATCTCTTTCCGTGACCAAGAGACTCAATCCTAATAGTGTTTCTCTAAGGTGAATCAATGATGACTCAATCCCAACCCTAGTGTTTGTTGCAAAGAGAAAACTCTACAAACCCTAGTTTTGTTGTGGTTTCTAAACCCTTGTTTTTCTACTCTCTACATCTCTCACCTTTCATCTGATACAAGGTTTATATTTATAGTAAAAGCATCCCTAAAAAATTGTAACACATCTCATGCTGAAAATACGTTTCTGTTTTTTCTGCATCATTAAAAACCAGACTCCGATTTTCTCACTTCGGGCGCAGATTTTTACGAGACAGAAGTCTCAAAACCACACCTAAAATTGGGCCTCGATTGCTCCAAATCGGCCGTCGATTTTGGCAGTTCCAGAAACACCATGTTTAAATCATACTAGAACATGAACTACAACCTATTTATCATAGGCTATGTAAATGTATCCGGAGTCTGATATGTATTAGTGTCTGATACAATTTGACGCATGTCATGTGTTAGTGTATATTTATGCTTCATAGGTTATAAGTCTTCTAGTGTCCCGAATGTCTAAGCTACCTCAAAAAAAAAAAGAAAAAAACTACCAGTAAAGCTACCTACTCACACACATATCAGTTGACAATTTTCTTGATTTTAAATACATAAATTCATTCTTTTGCCAAGACTGTTGTGGGTGTGTTTGTTTTGGCTTAAAATAAAATTATTTTGTTTTTATAAAATATAAATATTATTTTTGAGATCTATAAAAAAAAAAGCCGAAGATAATTTCCATTTATTTACTATCATAAAAATAATTTTTTTAAGATTTAAAACACTTACACTTGAGTTTCTTGTTTTTTAAAAGAAAAATAGATTTTGAAAAAGCTATTTGAATAACTTTTCGATTTGAGGCTAAAAATTACTCCATTTTTTTAACAAAATATTTTTAAAAAAAATCTGAAACAAACACTATAAAATTATATAAGTATTTTTTTGGTTTAAAAAATTGATTTTTTGTTTCTTTTAAAAAAATCTAAAACAAAAGGGTCTGTGTTTTCTCTACTTCTAAAGTTGAAATTTTAAACTTTTTAATGATTATTACTGCTTTATTGGGCCTGACATGTTAGTGATTTTTTTTTTTCCTTCTTTTTCTGAATGGCAAAAGAAAATTTTATAAAATAAGATGATACTAATCTTATTTAATAAAATTTTCATATGAGAATTGCTGGTCTTTTATATAATAATGCTGGAAGCCTTTTTATTTAAATGTCTCTTCACTAAATCACTAGCTTGATTCTTGGTTGCATTGTTGCAGTTACTACAACTACGAAGTCATTGGAAGCGCTTGAATTGTTGAGAGAAAAGAAGGATATGTTTGACCTTGTAATTAGTGATGTTAGCATGCCAGACATGGATGGCTTCAAGCTCCTTCAGCAAGTCGGACTTGAAATGGACCTACCTTTCATCAGTAAGCTCACACACCTTTCTCTTAAAATCTGTTTTGTTTACCTAATTAAGATCCATTTCTTATATTCGGATATTTTGTGTGTGTGATTTATAGTGTTGTCGGTAAATGATGATATAGAGAAGGTGATGAAGAGTGTGATACATGGTGCTTGCAACTATCTAGTGAAACCGATTCGAATGGAAGAGCTTAAGAGCATATGGCAACATGTAGTTAGAAAGAAGATTGAAAGCAAGGATCAAAATCAAGGTATCATTAGTGATGGGGTATATGGTCAAGACACCTCATCCGAAAATATTGCTAACAAGAATAAAACGCATGGACAAAAAAGAAAGGTACAAAGTGAGGAGGAAGAAGCAGAACAAGATAATGATGAGAAACGTTCGCCTCAAAAGAAGCCTCGTTTAGTTTGGGATCATGAGTTGCATCGAAAATTTGTTTCTGCAGTTAATCTTGTCGGCCTTGACAGTGAGTTTATGTGACAATGAAATTATTTTTTTACTTTGCTAATTTGATGAATGATTCTTAAATATGGATATTATTGTTCCTAATATGTAGTTTCTTCAACTATTATTGCAGAGGCTTCGCCTAAGAAAATACTTGACTTGATGAATGTTGAAGGGCTTACAAGGGAAAATGTATCCAGCCATTTGCAGGTACAAATTATATATAACTTTCCCTTTTGTCAAAAAATCTCAAACTAGTTTATCATTAAAATATTCACCTCCTTTTTGCAAATACGGTTGAAACTTCAACAAGCAAGTTTATGAAACGTAAGTATAAAAACTGTGTGTAACCTCATTTTCATTTTCGATTGCAGAGATACAGAATTGATATCAAATGGCTATCTAAACAGGATAGAGGAGATGATGCGTTGGATCCCTACCAGCAAAAGGGTTCAGTAGGTGGATATGGGGATTTTTGCACACTGCCAGCATATGCATCCAATGACATGTTTTGTCGGCTGAACACACCATCCAGTTTGAACTTGGAAGGAATGAGTTCTTCTGCACTAGTCCCTCCACTTCAGTCTCATAACATTCCCAATTTCGAGACACCAATGTTTTCTGAAAGTGAAAGTTCGAGTTCTTTACATGGCATTCAAACGTCAATGGAAATTAACCAGTTTCAGCAGAATATTTATCCGCCTGATAACATTAATTTAAGTCCAATTGATGATTCAAATGCAGTTACAGATTCCTCTGGCTTCCAAGACATCAGAGCCACTGTCAATAATGCAAATGTTTCTCTTTCTTGCATCGCAAGCAATCACATACAAACACATAAATCAGAAGCATTTATAAATCACTCTCCTGTTGAAGGTTCTGCTGTGAAAACACAACCTTTTAACCCTGTCAGAAGTGGTTCCTCTAACTTTGCTTCCAATTCTGTTCCATTGAGTGAGGATTTTAACAATGATCAAATGTTGCACAATAACTACAAGTTTACTTCTCCCAGTTCTCATTTTTCTTCGACAGACATCGTTGCTGTTCCTTTGGAGGAAACGATGCAATGTCAAGATGGCTTACTTGGGAATGTTATAAATGCTCCATGTTACTCACAACATCAAAATGCGAGTTCCTCCTTCAACAATACCTTGGATTATCTTGCTTCTTCTAATGGAGACACTAGTTCCATGGTACAAAGTTTAGCCCAAACTAATAGTTCATTAATCACTCAGATGCCTCAAGTTGAGAAGTTCTATTCGGATCAAGGAATCATGGAATCAAATGGTGATTGCTTTATTGAGCAATTGCTTAATGTAGATGGATTCATTCAGAATAGTCGTGTGCCTTGGACAACACAATAA
Protein sequence

>MS.gene061197.t1

MVVEDSFGVDDVLGEGFPAGMRVLAVDDDPTYLKVLEKQLLTCNYNVTTTTKSLEALELLREKKDMFDLVISDVSMPDMDGFKLLQQVGLEMDLPFIMLSVNDDIEKVMKSVIHGACNYLVKPIRMEELKSIWQHVVRKKIESKDQNQGIISDGVYGQDTSSENIANKNKTHGQKRKVQSEEEEAEQDNDEKRSPQKKPRLVWDHELHRKFVSAVNLVGLDKASPKKILDLMNVEGLTRENVSSHLQRYRIDIKWLSKQDRGDDALDPYQQKGSVGGYGDFCTLPAYASNDMFCRLNTPSSLNLEGMSSSALVPPLQSHNIPNFETPMFSESESSSSLHGIQTSMEINQFQQNIYPPDNINLSPIDDSNAVTDSSGFQDIRATVNNANVSLSCIASNHIQTHKSEAFINHSPVEGSAVKTQPFNPVRSGSSNFASNSVPLSEDFNNDQMLHNNYKFTSPSSHFSSTDIVAVPLEETMQCQDGLLGNVINAPCYSQHQNASSSFNNTLDYLASSNGDTSSMVQSLAQTNSSLITQMPQVEKFYSDQGIMESNGDCFIEQLLNVDGFIQNSRVPWTTQ