AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061199

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061199.t1 MTR_4g131580 72.513 593 104 5 1 537 1 590 0.0 822
MS.gene061199.t1 MTR_4g131570 92.982 399 24 2 144 542 202 596 0.0 758
MS.gene061199.t1 MTR_4g131600 65.042 595 147 3 1 542 4 590 0.0 756
MS.gene061199.t1 MTR_2g450070 32.548 679 278 23 3 533 4 650 2.90e-76 254
MS.gene061199.t1 MTR_2g450070 32.548 679 278 23 3 533 4 650 5.72e-76 254
MS.gene061199.t1 MTR_3g102600 49.801 251 94 3 11 261 2 220 8.90e-73 231
MS.gene061199.t1 MTR_2g034960 40.678 354 144 5 6 295 18 369 9.01e-73 243
MS.gene061199.t1 MTR_3g102600 59.140 186 72 1 11 196 2 183 2.74e-68 218
MS.gene061199.t1 MTR_8g079940 49.593 246 68 2 17 206 25 270 8.35e-67 226
MS.gene061199.t1 MTR_1g032570 42.230 296 99 4 16 242 10 302 2.52e-65 224
MS.gene061199.t1 MTR_4g121020 39.062 384 145 12 15 320 29 401 9.19e-64 221
MS.gene061199.t1 MTR_4g121020 39.062 384 145 12 15 320 29 401 9.64e-64 221
MS.gene061199.t1 MTR_3g102590 57.672 189 73 2 17 198 8 196 5.82e-61 201
MS.gene061199.t1 MTR_3g102590 57.297 185 72 2 17 194 8 192 5.39e-58 192
MS.gene061199.t1 MTR_3g086100 45.968 248 76 2 16 205 12 259 1.44e-57 202
MS.gene061199.t1 MTR_3g102600 48.276 203 73 3 59 261 1 171 4.62e-56 186
MS.gene061199.t1 MTR_3g106220 64.234 137 48 1 14 149 5 141 4.95e-53 191
MS.gene061199.t1 MTR_3g106220 45.714 140 64 2 144 279 197 328 1.17e-24 108
MS.gene061199.t1 MTR_1g013170 63.910 133 48 0 15 147 3 135 6.62e-53 187
MS.gene061199.t1 MTR_1g013170 43.448 145 73 2 144 279 190 334 9.51e-23 102
MS.gene061199.t1 MTR_3g102600 60.145 138 51 1 59 196 1 134 1.21e-52 176
MS.gene061199.t1 MTR_1g013180 58.784 148 60 1 2 148 140 287 6.27e-50 182
MS.gene061199.t1 MTR_1g013180 42.105 133 71 4 144 270 341 473 1.03e-18 90.1
MS.gene061199.t1 MTR_7g117705 61.194 134 51 1 15 147 3 136 5.26e-48 173
MS.gene061199.t1 MTR_7g117705 62.069 58 22 0 144 201 190 247 5.39e-17 84.0
MS.gene061199.t1 MTR_1g032570 40.254 236 69 4 76 242 1 233 1.09e-41 157
MS.gene061199.t1 MTR_4g021790 35.426 223 112 3 17 208 10 231 1.35e-38 142
MS.gene061199.t1 MTR_4g021760 33.184 223 117 2 17 208 11 232 2.23e-38 143
MS.gene061199.t1 MTR_4g021855 34.081 223 115 2 17 208 10 231 7.06e-38 141
MS.gene061199.t1 MTR_4g021845 35.407 209 103 3 17 194 10 217 6.81e-35 131
MS.gene061199.t1 MTR_6g045327 31.602 231 125 3 1 201 1 228 3.31e-34 133
MS.gene061199.t1 MTR_0450s0040 34.247 219 105 4 21 201 8 225 5.14e-33 129
MS.gene061199.t1 MTR_3g086100 41.489 188 52 2 76 205 1 188 1.04e-32 131
MS.gene061199.t1 MTR_8g105600 31.535 241 113 3 13 202 5 244 3.73e-30 124
MS.gene061199.t1 MTR_5g055260 36.041 197 107 5 17 202 10 198 2.82e-28 114
MS.gene061199.t1 MTR_4g121020 32.673 303 115 12 96 320 6 297 1.28e-26 114
MS.gene061199.t1 MTR_7g026400 31.884 207 99 2 47 211 17 223 1.69e-26 114
MS.gene061199.t1 MTR_1g013160 69.565 69 21 0 79 147 1 69 1.36e-25 103
MS.gene061199.t1 MTR_8g093040 26.966 267 154 6 1 244 1 249 7.98e-22 95.9
MS.gene061199.t1 MTR_3g092780 34.921 126 79 2 21 143 56 181 1.67e-21 99.0
MS.gene061199.t1 MTR_3g092780 34.921 126 79 2 21 143 56 181 2.13e-21 98.6
MS.gene061199.t1 MTR_6g016850 33.813 139 89 2 13 149 5 142 6.78e-21 90.5
MS.gene061199.t1 MTR_7g118260 35.385 130 81 2 18 144 11 140 6.94e-21 96.7
MS.gene061199.t1 MTR_3g092780 34.959 123 77 2 21 140 56 178 8.09e-21 96.7
MS.gene061199.t1 MTR_3g092780 34.959 123 77 2 21 140 56 178 8.18e-21 96.7
MS.gene061199.t1 MTR_8g024260 37.600 125 75 2 23 144 38 162 8.79e-21 96.7
MS.gene061199.t1 MTR_7g118260 32.919 161 99 4 18 169 11 171 1.19e-20 95.9
MS.gene061199.t1 MTR_4g064730 55.844 77 34 0 126 202 112 188 3.51e-19 89.0
MS.gene061199.t1 MTR_4g061360 36.691 139 81 4 5 139 75 210 5.46e-19 91.3
MS.gene061199.t1 MTR_1g067110 36.885 122 74 2 21 139 85 206 1.94e-18 89.4
MS.gene061199.t1 MTR_1g067110 36.885 122 74 2 21 139 85 206 2.09e-18 89.4
MS.gene061199.t1 MTR_3g037390 31.206 141 92 2 3 140 8 146 3.07e-17 85.5
MS.gene061199.t1 MTR_4g108880 31.707 123 81 1 21 140 33 155 6.30e-17 84.3
MS.gene061199.t1 MTR_7g089010 62.712 59 22 0 144 202 90 148 7.48e-17 81.6
MS.gene061199.t1 MTR_4g023980 29.927 137 65 2 63 168 35 171 2.09e-16 77.8
MS.gene061199.t1 MTR_8g032710 29.412 136 91 2 20 150 20 155 1.83e-15 79.3
MS.gene061199.t1 MTR_4g098870 37.302 126 75 2 15 139 12 134 4.24e-15 78.6
MS.gene061199.t1 MTR_4g098870 67.347 49 16 0 150 198 315 363 4.59e-14 75.1
MS.gene061199.t1 MTR_4g098870 37.302 126 75 2 15 139 32 154 6.11e-15 78.2
MS.gene061199.t1 MTR_4g098870 67.347 49 16 0 150 198 335 383 4.98e-14 75.1
MS.gene061199.t1 MTR_4g098870 37.302 126 75 2 15 139 51 173 6.67e-15 78.2
MS.gene061199.t1 MTR_4g098870 67.347 49 16 0 150 198 354 402 5.61e-14 75.1
MS.gene061199.t1 MTR_4g098870 37.302 126 75 2 15 139 12 134 8.74e-15 77.4
MS.gene061199.t1 MTR_4g098870 67.347 49 16 0 150 198 315 363 6.14e-14 74.7
MS.gene061199.t1 MTR_5g014040 37.302 126 75 2 15 139 12 134 1.04e-14 77.4
MS.gene061199.t1 MTR_5g014040 55.172 58 21 1 150 202 316 373 1.02e-11 67.8
MS.gene061199.t1 MTR_4g098870 37.302 126 75 2 15 139 32 154 1.05e-14 77.4
MS.gene061199.t1 MTR_4g098870 67.347 49 16 0 150 198 335 383 6.26e-14 75.1
MS.gene061199.t1 MTR_5g014040 37.302 126 75 2 15 139 12 134 1.09e-14 77.0
MS.gene061199.t1 MTR_5g014040 60.377 53 21 0 150 202 316 368 1.18e-13 73.9
MS.gene061199.t1 MTR_1g093080 49.254 67 33 1 139 204 205 271 5.82e-13 70.9
MS.gene061199.t1 MTR_4g078810 56.250 48 21 0 150 197 163 210 3.17e-12 68.9
MS.gene061199.t1 MTR_1g049100 28.889 135 82 3 21 141 8 142 3.49e-12 65.9
MS.gene061199.t1 MTR_7g490310 28.571 154 87 3 22 154 10 161 4.13e-12 66.6
MS.gene061199.t1 MTR_2g090675 56.250 48 21 0 150 197 166 213 6.11e-12 68.2
MS.gene061199.t1 MTR_5g014040 55.172 58 21 1 150 202 276 333 8.01e-12 68.2
MS.gene061199.t1 MTR_4g106590 27.083 144 89 3 15 142 17 160 1.06e-11 65.1
MS.gene061199.t1 MTR_6g088950 31.481 108 69 1 47 154 34 136 2.85e-11 63.5
MS.gene061199.t1 MTR_1874s0010 31.858 113 73 2 19 129 2 112 4.49e-11 63.5
MS.gene061199.t1 MTR_4g086835 46.667 60 32 0 143 202 272 331 6.00e-11 65.1
MS.gene061199.t1 MTR_8g038620 25.694 144 87 3 22 145 10 153 7.86e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061199.t1 AT4G31920 47.040 321 113 8 1 268 1 317 7.11e-78 255
MS.gene061199.t1 AT5G58080 43.288 365 139 7 9 314 6 361 5.34e-73 244
MS.gene061199.t1 AT1G67710 48.583 247 68 7 18 205 8 254 8.43e-56 196
MS.gene061199.t1 AT2G25180 65.493 142 45 1 1 142 1 138 3.05e-55 196
MS.gene061199.t1 AT2G25180 40.659 182 96 6 149 319 199 379 5.09e-23 103
MS.gene061199.t1 AT3G16857 61.905 126 48 0 15 140 31 156 5.33e-46 171
MS.gene061199.t1 AT3G16857 51.111 90 44 0 144 233 236 325 9.72e-20 93.6
MS.gene061199.t1 AT3G16857 61.905 126 48 0 15 140 31 156 6.69e-46 171
MS.gene061199.t1 AT3G16857 40.152 132 75 2 144 271 236 367 9.03e-20 93.6
MS.gene061199.t1 AT2G01760 55.814 129 56 1 13 140 3 131 1.37e-41 154
MS.gene061199.t1 AT2G01760 61.538 65 25 0 143 207 198 262 9.54e-18 85.9
MS.gene061199.t1 AT5G49240 35.270 241 99 2 11 194 32 272 8.58e-40 146
MS.gene061199.t1 AT2G01760 46.452 155 54 2 15 140 5 159 8.93e-36 138
MS.gene061199.t1 AT2G01760 61.538 65 25 0 143 207 226 290 1.06e-17 85.9
MS.gene061199.t1 AT1G49190 29.316 307 138 9 17 260 30 320 9.07e-33 130
MS.gene061199.t1 AT3G16857 68.235 85 27 0 56 140 2 86 2.63e-32 131
MS.gene061199.t1 AT3G16857 40.152 132 75 2 144 271 166 297 6.92e-20 94.0
MS.gene061199.t1 AT1G67710 44.444 189 46 7 76 205 1 189 4.15e-31 126
MS.gene061199.t1 AT3G62670 32.714 269 118 8 36 249 54 314 6.72e-31 125
MS.gene061199.t1 AT1G49190 38.400 125 77 0 17 141 30 154 7.10e-24 106
MS.gene061199.t1 AT2G46790 37.209 129 78 2 21 146 37 165 5.47e-22 99.4
MS.gene061199.t1 AT2G46790 37.209 129 78 2 21 146 37 165 6.84e-22 99.4
MS.gene061199.t1 AT5G24470 36.000 125 77 2 19 140 48 172 7.49e-22 99.8
MS.gene061199.t1 AT2G01760 65.152 66 22 1 76 140 1 66 1.84e-20 92.8
MS.gene061199.t1 AT2G01760 61.538 65 25 0 143 207 133 197 4.77e-18 85.9
MS.gene061199.t1 AT5G60100 37.795 127 75 3 17 139 59 185 2.36e-19 90.9
MS.gene061199.t1 AT5G60100 37.795 127 75 3 17 139 59 185 2.36e-19 90.9
MS.gene061199.t1 AT5G60100 37.795 127 75 3 17 139 59 185 2.36e-19 90.9
MS.gene061199.t1 AT5G60100 37.795 127 75 3 17 139 59 185 4.50e-19 90.9
MS.gene061199.t1 AT5G60100 37.795 127 75 3 17 139 59 185 4.50e-19 90.9
MS.gene061199.t1 AT5G60100 37.795 127 75 3 17 139 59 185 4.50e-19 90.9
MS.gene061199.t1 AT5G59570 66.667 60 20 0 143 202 139 198 5.41e-19 88.2
MS.gene061199.t1 AT5G59570 66.667 60 20 0 143 202 139 198 5.41e-19 88.2
MS.gene061199.t1 AT5G60100 37.795 127 75 3 17 139 59 185 6.22e-19 90.5
MS.gene061199.t1 AT1G67710 39.286 168 43 7 97 205 13 180 6.31e-19 90.1
MS.gene061199.t1 AT3G46640 66.102 59 20 0 144 202 143 201 1.46e-18 87.4
MS.gene061199.t1 AT3G46640 66.102 59 20 0 144 202 143 201 1.46e-18 87.4
MS.gene061199.t1 AT3G46640 66.102 59 20 0 144 202 143 201 1.82e-18 87.0
MS.gene061199.t1 AT2G40970 62.712 59 22 0 144 202 104 162 3.09e-17 82.0
MS.gene061199.t1 AT5G05090 62.712 59 22 0 144 202 80 138 3.09e-17 82.4
MS.gene061199.t1 AT5G02810 36.885 122 74 2 21 139 78 199 3.85e-17 85.5
MS.gene061199.t1 AT3G10760 62.712 59 22 0 144 202 104 162 5.05e-17 82.8
MS.gene061199.t1 AT5G61380 33.333 123 79 1 21 140 19 141 1.12e-16 83.6
MS.gene061199.t1 AT5G44190 57.627 59 25 0 144 202 146 204 5.32e-14 73.9
MS.gene061199.t1 AT5G44190 57.627 59 25 0 144 202 146 204 6.11e-14 73.9
MS.gene061199.t1 AT4G18020 39.394 132 71 3 15 139 12 141 6.75e-14 74.7
MS.gene061199.t1 AT4G18020 53.125 64 23 1 138 201 296 352 7.40e-12 68.2
MS.gene061199.t1 AT4G18020 39.394 132 71 3 15 139 12 141 6.75e-14 74.7
MS.gene061199.t1 AT4G18020 53.125 64 23 1 138 201 296 352 7.40e-12 68.2
MS.gene061199.t1 AT4G18020 39.394 132 71 3 15 139 12 141 6.75e-14 74.7
MS.gene061199.t1 AT4G18020 53.125 64 23 1 138 201 296 352 7.40e-12 68.2
MS.gene061199.t1 AT4G18020 39.394 132 71 3 15 139 12 141 6.75e-14 74.7
MS.gene061199.t1 AT4G18020 53.125 64 23 1 138 201 296 352 7.40e-12 68.2
MS.gene061199.t1 AT4G18020 39.394 132 71 3 15 139 12 141 6.75e-14 74.7
MS.gene061199.t1 AT4G18020 53.125 64 23 1 138 201 296 352 7.40e-12 68.2
MS.gene061199.t1 AT4G18020 39.394 132 71 3 15 139 12 141 6.75e-14 74.7
MS.gene061199.t1 AT4G18020 53.125 64 23 1 138 201 296 352 7.40e-12 68.2
MS.gene061199.t1 AT4G18020 39.394 132 71 3 15 139 12 141 8.61e-14 74.3
MS.gene061199.t1 AT4G18020 53.125 64 23 1 138 201 296 352 8.10e-12 68.2
MS.gene061199.t1 AT4G18020 39.394 132 71 3 15 139 12 141 8.61e-14 74.3
MS.gene061199.t1 AT4G18020 53.125 64 23 1 138 201 296 352 8.10e-12 68.2
MS.gene061199.t1 AT4G18020 39.394 132 71 3 15 139 12 141 8.61e-14 74.3
MS.gene061199.t1 AT4G18020 53.125 64 23 1 138 201 296 352 8.10e-12 68.2
MS.gene061199.t1 AT4G18020 39.394 132 71 3 15 139 12 141 8.61e-14 74.3
MS.gene061199.t1 AT4G18020 53.125 64 23 1 138 201 296 352 8.10e-12 68.2
MS.gene061199.t1 AT4G18020 39.394 132 71 3 15 139 12 141 8.61e-14 74.3
MS.gene061199.t1 AT4G18020 53.125 64 23 1 138 201 296 352 8.10e-12 68.2
MS.gene061199.t1 AT5G07210 48.649 74 31 1 134 207 218 284 2.92e-13 72.8
MS.gene061199.t1 AT5G07210 30.400 125 84 2 21 144 16 138 3.97e-13 72.4
MS.gene061199.t1 AT5G07210 48.649 74 31 1 134 207 226 292 3.06e-13 72.8
MS.gene061199.t1 AT2G20570 59.615 52 21 0 150 201 158 209 1.75e-12 69.7
MS.gene061199.t1 AT2G20570 59.615 52 21 0 150 201 174 225 1.97e-12 69.7
MS.gene061199.t1 AT2G03500 43.662 71 39 1 148 217 236 306 5.77e-12 68.2
MS.gene061199.t1 AT1G19050 27.389 157 99 3 1 142 1 157 1.60e-11 64.3
MS.gene061199.t1 AT2G27070 52.542 59 28 0 150 208 230 288 1.75e-11 67.4
MS.gene061199.t1 AT1G10470 30.000 130 79 3 21 138 20 149 3.96e-11 63.9
MS.gene061199.t1 AT1G10470 30.000 130 79 3 21 138 34 163 4.77e-11 63.9

Find 109 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCGCTCAGTTCTCAATTT+AGG 0.173828 8.4:+1993139 MS.gene061199:CDS
AGCTTGAATCCATCCATTTC+TGG 0.203555 8.4:-1991429 None:intergenic
TTATAGTTTGTACCTGTAAA+TGG 0.224166 8.4:-1992222 None:intergenic
TGAAAACTCGAGTGCTTTAA+AGG 0.227005 8.4:+1992765 MS.gene061199:CDS
AAACAATGGCTGCTTGTAAC+TGG 0.250042 8.4:-1992734 None:intergenic
CGTCATTGATGTTCCTTTAG+AGG 0.255703 8.4:+1993191 MS.gene061199:CDS
TATTAGCTTGGGGTACATTA+TGG 0.266193 8.4:-1993353 None:intergenic
TCCTGTGGGAATGCATGTTC+TGG 0.298859 8.4:+1990294 MS.gene061199:CDS
CTTCTCATGTGCAAGTATAA+TGG 0.318350 8.4:+1990361 MS.gene061199:CDS
CAATGTCAAGATGGATTACT+TGG 0.334290 8.4:+1993222 MS.gene061199:CDS
GAGAAAGAAGATTGAAAGTA+AGG 0.340472 8.4:+1991723 MS.gene061199:CDS
TGAGGCATCTGAGTGATTAA+TGG 0.341513 8.4:-1993378 None:intergenic
CATTGCATTGTTTCCTCTAA+AGG 0.359272 8.4:-1993204 None:intergenic
AAGAATTCAGTAGCTGGATA+TGG 0.366836 8.4:+1992550 MS.gene061199:CDS
AGTGCATTTACAGATTCTTC+TGG 0.369412 8.4:+1992874 MS.gene061199:CDS
TTATTCAGATGAAAGAACTA+TGG 0.373116 8.4:+1993413 MS.gene061199:CDS
AGGACTGGAAGCAAAGTTAT+AGG 0.378746 8.4:-1993058 None:intergenic
GATGGCTTTGGAGAATCCTT+TGG 0.383986 8.4:+1990243 None:intergenic
TCTCAAAAGGCCATCTAAAC+AGG 0.387755 8.4:+1992492 MS.gene061199:CDS
GATAAATGTTCCGGTGAAAC+TGG 0.391840 8.4:-1992813 None:intergenic
TCAGTAATGATACCTTTATT+CGG 0.392934 8.4:-1991748 None:intergenic
AAATGTGGCTAGCCATTTAC+AGG 0.398143 8.4:+1992210 MS.gene061199:CDS
CTCTTGAATTATGTGTTTGT+AGG 0.401274 8.4:-1992963 None:intergenic
TCTACTCTAGCCTGTTTAGA+TGG 0.401513 8.4:-1992502 None:intergenic
ATTCTGGTTCATGATGGCTT+TGG 0.405441 8.4:+1990231 None:intergenic
GAAAGTAAGGATCCGAATAA+AGG 0.416092 8.4:+1991736 MS.gene061199:CDS
TTCAGAATAGTTGTTTGCCT+TGG 0.419816 8.4:+1993490 MS.gene061199:CDS
CTTAAGATTCTTCTTGATCC+AGG 0.420041 8.4:-1992589 None:intergenic
TGTCGGCTGAACGCACCATC+TGG 0.422911 8.4:+1992649 MS.gene061199:CDS
TGAGCAAGTTGGACATGAAA+TGG 0.425491 8.4:+1991455 MS.gene061199:CDS
GAGCAATTGCTTGATGTAGA+TGG 0.429100 8.4:+1993462 MS.gene061199:CDS
TTTATAAATCACCCATCTGT+TGG 0.434200 8.4:+1992988 MS.gene061199:CDS
AATGCACTTGAATCATCAAT+TGG 0.435178 8.4:-1992859 None:intergenic
GATTAATGGACTATTAGCTT+GGG 0.435758 8.4:-1993364 None:intergenic
TACCAGGTGGATAAATGTTC+CGG 0.439267 8.4:-1992822 None:intergenic
CATCTAGTAACATGTATTGT+CGG 0.449068 8.4:+1992632 MS.gene061199:CDS
TCTCTTGCTTCTTCTAATGC+AGG 0.449172 8.4:+1993315 MS.gene061199:CDS
TAACATTAGCTCTGATGTCT+TGG 0.449900 8.4:-1992900 None:intergenic
CACCGGAACATTTATCCACC+TGG 0.455890 8.4:+1992820 MS.gene061199:CDS
TTGGAGGTTGAAGATGTCTT+CGG 0.457510 8.4:+1990262 MS.gene061199:CDS
TTATAGGAACCACTTGTAGC+AGG 0.464041 8.4:-1993042 None:intergenic
AATACAAACTTAGTCTCAAA+AGG 0.473606 8.4:+1992479 MS.gene061199:CDS
TGGAAGAGCTTAAGAACATA+TGG 0.475204 8.4:+1991689 MS.gene061199:CDS
GGAGATTTGTGTACCTTACC+TGG 0.477382 8.4:+1992571 MS.gene061199:CDS
TGATTAATGGACTATTAGCT+TGG 0.477988 8.4:-1993365 None:intergenic
TGATGTAAACATGCCAGAAA+TGG 0.478571 8.4:+1991416 MS.gene061199:CDS
TTCTTCCTTCAACAATACCT+TGG 0.480791 8.4:+1993290 MS.gene061199:CDS
TTAAGCTCTTCCAATCGGAT+TGG 0.487989 8.4:-1991679 None:intergenic
GATAGAGAGAGGGTGATGAA+AGG 0.489158 8.4:+1991622 MS.gene061199:CDS
TCAAACTTGTCTCAAACTGT+TGG 0.489304 8.4:+1990330 MS.gene061199:CDS
TGTTCTTAAGCTCTTCCAAT+CGG 0.493421 8.4:-1991684 None:intergenic
GGCTTTGGAGAATCCTTTGG+AGG 0.494937 8.4:+1990246 MS.gene061199:CDS
CTCACTCAATGGAGAAGGAC+TGG 0.495705 8.4:-1993073 None:intergenic
TGAATGTTGAAGGGCTTACA+AGG 0.497287 8.4:+1992185 MS.gene061199:CDS
GAAAGAACTATGGAATCAAA+TGG 0.497837 8.4:+1993423 MS.gene061199:CDS
CAGTCCTTCTCCATTGAGTG+AGG 0.499289 8.4:+1993074 MS.gene061199:CDS
CTACAAAAGAATTCAGTAGC+TGG 0.500934 8.4:+1992544 MS.gene061199:CDS
ATCTGTTGGAGGTCCTGCTA+TGG 0.503540 8.4:+1993002 MS.gene061199:CDS
GAAAACTCGAGTGCTTTAAA+GGG 0.504861 8.4:+1992766 MS.gene061199:CDS
AGAATCCAAGGTATTGTTGA+AGG 0.506294 8.4:-1993295 None:intergenic
TTATTAAAATCCTCACTCAA+TGG 0.507785 8.4:-1993084 None:intergenic
CAGGAAATGATGATAGAGAG+AGG 0.513398 8.4:+1991611 MS.gene061199:CDS
TGTGATCATATAGTGTTGTC+AGG 0.513792 8.4:+1991592 MS.gene061199:intron
AGAAGAAGCAAGAGAATCCA+AGG 0.514940 8.4:-1993307 None:intergenic
GGACTTAAGTTCATGTTACC+AGG 0.516544 8.4:-1992838 None:intergenic
TTCAAGCTCCTTGAGCAAGT+TGG 0.519096 8.4:+1991444 MS.gene061199:CDS
GATTCTTCTTGATCCAGGTA+AGG 0.519260 8.4:-1992584 None:intergenic
AACAATGGCTGCTTGTAACT+GGG 0.528436 8.4:-1992733 None:intergenic
GAATAAAATTTCTCAACTTG+AGG 0.529905 8.4:-1993396 None:intergenic
GTCACATAAACTCACTATCA+AGG 0.536123 8.4:-1991996 None:intergenic
TTCACTTATTGTATCATCCA+AGG 0.536142 8.4:-1993507 None:intergenic
GAAACAATGCAATGTCAAGA+TGG 0.544924 8.4:+1993213 MS.gene061199:CDS
TCTTGTGAAACCAATCCGAT+TGG 0.545613 8.4:+1991669 MS.gene061199:CDS
TGTGACTTGATGAATGTTGA+AGG 0.547972 8.4:+1992175 MS.gene061199:CDS
ATAAATCACCCATCTGTTGG+AGG 0.552382 8.4:+1992991 MS.gene061199:CDS
TTGAAGCGCTAGAATTGTTG+AGG 0.553092 8.4:+1991361 MS.gene061199:CDS
GTGTGTGAGCTTACTGATGA+AGG 0.556710 8.4:-1991484 None:intergenic
TAATGCAGGCACTAGTTCCA+TGG 0.559067 8.4:+1993329 MS.gene061199:CDS
TGAAGCGCTAGAATTGTTGA+GGG 0.561694 8.4:+1991362 MS.gene061199:CDS
AGGAAATGATGATAGAGAGA+GGG 0.563967 8.4:+1991612 MS.gene061199:CDS
TTTCATGTCCAACTTGCTCA+AGG 0.564395 8.4:-1991452 None:intergenic
TGGAAATTAACCAGTTTCAC+CGG 0.564960 8.4:+1992803 MS.gene061199:CDS
ATGTTACTAGATGCATATGT+TGG 0.572114 8.4:-1992622 None:intergenic
AAATCCTCACTCAATGGAGA+AGG 0.576468 8.4:-1993078 None:intergenic
ATAGCAGGACCTCCAACAGA+TGG 0.581262 8.4:-1993000 None:intergenic
CCATCTGGATTGAACTTGAG+AGG 0.585944 8.4:+1992664 MS.gene061199:CDS
TATAGGAACCACTTGTAGCA+GGG 0.588786 8.4:-1993041 None:intergenic
AAAGGGTGTTCGAACGTCAG+TGG 0.592037 8.4:+1992783 MS.gene061199:CDS
CCTCTCAAGTTCAATCCAGA+TGG 0.601365 8.4:-1992664 None:intergenic
GTAAACATGCCAGAAATGGA+TGG 0.605525 8.4:+1991420 MS.gene061199:CDS
GTGATGAAAGGAGTGATGAA+AGG 0.606414 8.4:+1991634 MS.gene061199:CDS
TAGCAGGACCTCCAACAGAT+GGG 0.607030 8.4:-1992999 None:intergenic
AAGACATCTTCAACCTCCAA+AGG 0.613959 8.4:-1990259 None:intergenic
CCTAAATTGAGAACTGAGCG+AGG 0.617995 8.4:-1993139 None:intergenic
GCCAGAACATGCATTCCCAC+AGG 0.618067 8.4:-1990295 None:intergenic
AGGGCTTACAAGGGAAAATG+TGG 0.634196 8.4:+1992195 MS.gene061199:CDS
ATTAATGGACTATTAGCTTG+GGG 0.634710 8.4:-1993363 None:intergenic
GTATCTATAGTTAGTCAACT+CGG 0.636343 8.4:+1991973 MS.gene061199:CDS
ATTACTTGGGAATGTTGTAG+CGG 0.641596 8.4:+1993236 MS.gene061199:CDS
TGAGCTTACTGATGAAGGGT+AGG 0.644624 8.4:-1991479 None:intergenic
GTGACTTGATGAATGTTGAA+GGG 0.645884 8.4:+1992176 MS.gene061199:CDS
AATGTCAAGATGGATTACTT+GGG 0.659008 8.4:+1993223 MS.gene061199:CDS
TGTGTGAGCTTACTGATGAA+GGG 0.665662 8.4:-1991483 None:intergenic
CTTAAGTTCATGTTACCAGG+TGG 0.668465 8.4:-1992835 None:intergenic
GAATGTTGAAGGGCTTACAA+GGG 0.675399 8.4:+1992186 MS.gene061199:CDS
TTACACGCAGCAGCAAAACG+TGG 0.681302 8.4:+1993266 MS.gene061199:CDS
TTGGGGTACATTATGGACCA+TGG 0.694419 8.4:-1993346 None:intergenic
TACACGCAGCAGCAAAACGT+GGG 0.705438 8.4:+1993267 MS.gene061199:CDS
AGAACTATGGAATCAAATGG+TGG 0.707711 8.4:+1993426 MS.gene061199:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATGAAATATTTTGACAAAA+GGG - chr8.4:1992252-1992271 None:intergenic 15.0%
!!! TTATGTTTCTACTAATTTTT+TGG - chr8.4:1990817-1990836 None:intergenic 15.0%
!! AAAATGAGAAAACAGTATTT+AGG - chr8.4:1991085-1991104 None:intergenic 20.0%
!! AAAGTCGAAAATAAAAATAG+AGG + chr8.4:1990947-1990966 MS.gene061199:intron 20.0%
!! ATTCACTTCTTATATTCATA+TGG + chr8.4:1991543-1991562 MS.gene061199:intron 20.0%
!! TAGGTCAACATATATTATTA+GGG - chr8.4:1990775-1990794 None:intergenic 20.0%
!!! AAATATTTTCGATGTAATCT+TGG + chr8.4:1990692-1990711 MS.gene061199:intron 20.0%
!!! AATAATTCAGTTGTTTTGAT+TGG + chr8.4:1990984-1991003 MS.gene061199:intron 20.0%
!!! ATAAGATCACTACTTTTATT+GGG + chr8.4:1991186-1991205 MS.gene061199:intron 20.0%
!!! ATGAAATATTTTGACAAAAG+GGG - chr8.4:1992251-1992270 None:intergenic 20.0%
!!! GAGAGACATTTAAAATAAAA+AGG - chr8.4:1991286-1991305 None:intergenic 20.0%
!!! GTATGAAATATTTTGACAAA+AGG - chr8.4:1992253-1992272 None:intergenic 20.0%
!!! TAGTAAACCCTTTTTTTAAT+CGG + chr8.4:1990510-1990529 MS.gene061199:intron 20.0%
!!! TGTTACTTTACTAATTTGAT+TGG + chr8.4:1992034-1992053 MS.gene061199:intron 20.0%
! AAAAATAAAGGAACAAAGTG+AGG + chr8.4:1991834-1991853 MS.gene061199:intron 25.0%
! AATACAAACTTAGTCTCAAA+AGG + chr8.4:1992479-1992498 MS.gene061199:CDS 25.0%
! ACCCGAAATAAACAAAAAAT+TGG - chr8.4:1990723-1990742 None:intergenic 25.0%
! CCCGAAATAAACAAAAAATT+GGG - chr8.4:1990722-1990741 None:intergenic 25.0%
! CCGAAATAAACAAAAAATTG+GGG - chr8.4:1990721-1990740 None:intergenic 25.0%
! CTGAAAGAAAGAAGAAAAAA+TGG - chr8.4:1990394-1990413 None:intergenic 25.0%
! GAATAAAATTTCTCAACTTG+AGG - chr8.4:1993399-1993418 None:intergenic 25.0%
! GTAGGTCAACATATATTATT+AGG - chr8.4:1990776-1990795 None:intergenic 25.0%
! GTTGAAATTTCAACGAATAA+TGG - chr8.4:1992394-1992413 None:intergenic 25.0%
! TAATACACATGCTAAAACAA+AGG - chr8.4:1991509-1991528 None:intergenic 25.0%
! TCAGTAATGATACCTTTATT+CGG - chr8.4:1991751-1991770 None:intergenic 25.0%
! TGATGATTGAAACTTACATA+TGG + chr8.4:1992088-1992107 MS.gene061199:intron 25.0%
! TGTAAGTTTCAATCATCAAA+TGG - chr8.4:1992086-1992105 None:intergenic 25.0%
! TTATTAAAATCCTCACTCAA+TGG - chr8.4:1993087-1993106 None:intergenic 25.0%
! TTATTCAGATGAAAGAACTA+TGG + chr8.4:1993413-1993432 MS.gene061199:CDS 25.0%
! TTTGGCAAATATACTTCTTT+GGG - chr8.4:1990799-1990818 None:intergenic 25.0%
!! ATCAAGTCACATATTTTCTT+AGG - chr8.4:1992166-1992185 None:intergenic 25.0%
!! CTCTTTTATGAAACATAAGT+GGG + chr8.4:1992417-1992436 MS.gene061199:intron 25.0%
!! TAACTTATAAGCTTTGTAGT+CGG + chr8.4:1990608-1990627 MS.gene061199:intron 25.0%
!! TCTCTTTTATGAAACATAAG+TGG + chr8.4:1992416-1992435 MS.gene061199:intron 25.0%
!! TTATAGTTTGTACCTGTAAA+TGG - chr8.4:1992225-1992244 None:intergenic 25.0%
!! TTTTCACTTGCAAAAAACAA+TGG - chr8.4:1992751-1992770 None:intergenic 25.0%
!! TTTTGGCAAATATACTTCTT+TGG - chr8.4:1990800-1990819 None:intergenic 25.0%
!!! AATAAAGGTATCATTACTGA+TGG + chr8.4:1991751-1991770 MS.gene061199:intron 25.0%
!!! AGTAAACCCTTTTTTTAATC+GGG + chr8.4:1990511-1990530 MS.gene061199:intron 25.0%
!!! ATAAAGGTATCATTACTGAT+GGG + chr8.4:1991752-1991771 MS.gene061199:intron 25.0%
!!! ATTTGATTGGCTTTAAAACA+TGG + chr8.4:1992047-1992066 MS.gene061199:intron 25.0%
!!! CATAAGATCACTACTTTTAT+TGG + chr8.4:1991185-1991204 MS.gene061199:intron 25.0%
!!! CCCAATTTTTTGTTTATTTC+GGG + chr8.4:1990719-1990738 MS.gene061199:intron 25.0%
!!! CCCCAATTTTTTGTTTATTT+CGG + chr8.4:1990718-1990737 MS.gene061199:intron 25.0%
AAATATCGTCGAAGTTAAAG+GGG + chr8.4:1992311-1992330 MS.gene061199:intron 30.0%
AACTTTGCTAGCAATGAAAA+TGG + chr8.4:1990917-1990936 MS.gene061199:intron 30.0%
AATGCACTTGAATCATCAAT+TGG - chr8.4:1992862-1992881 None:intergenic 30.0%
ATGTTACTAGATGCATATGT+TGG - chr8.4:1992625-1992644 None:intergenic 30.0%
ATTTGCCCCGATTAAAAAAA+GGG - chr8.4:1990520-1990539 None:intergenic 30.0%
CAAATATCGTCGAAGTTAAA+GGG + chr8.4:1992310-1992329 MS.gene061199:intron 30.0%
CAGTGGTGTAAACTTATAAT+TGG + chr8.4:1990473-1990492 MS.gene061199:intron 30.0%
CATATGCTTGTATGCAAATA+TGG + chr8.4:1992350-1992369 MS.gene061199:intron 30.0%
CATCTAGTAACATGTATTGT+CGG + chr8.4:1992632-1992651 MS.gene061199:CDS 30.0%
CTCTTGAATTATGTGTTTGT+AGG - chr8.4:1992966-1992985 None:intergenic 30.0%
GAAAGAACTATGGAATCAAA+TGG + chr8.4:1993423-1993442 MS.gene061199:CDS 30.0%
GAGAAAGAAGATTGAAAGTA+AGG + chr8.4:1991723-1991742 MS.gene061199:CDS 30.0%
GTATCTATAGTTAGTCAACT+CGG + chr8.4:1991973-1991992 MS.gene061199:CDS 30.0%
GTCAACATATATTATTAGGG+TGG - chr8.4:1990772-1990791 None:intergenic 30.0%
TTGGCAAATATACTTCTTTG+GGG - chr8.4:1990798-1990817 None:intergenic 30.0%
TTTATAAATCACCCATCTGT+TGG + chr8.4:1992988-1993007 MS.gene061199:CDS 30.0%
! AAAGATTTTTGTTCCATAGC+AGG - chr8.4:1993018-1993037 None:intergenic 30.0%
! AGTTGAAGAAAGTACATGTT+AGG - chr8.4:1992125-1992144 None:intergenic 30.0%
! GTTAGCAATATTTTCAGATG+AGG - chr8.4:1991794-1991813 None:intergenic 30.0%
!! AATGTCAAGATGGATTACTT+GGG + chr8.4:1993223-1993242 MS.gene061199:CDS 30.0%
!! ATCATCAAATGGTCTTGTTA+AGG - chr8.4:1992075-1992094 None:intergenic 30.0%
!! ATTAATGGACTATTAGCTTG+GGG - chr8.4:1993366-1993385 None:intergenic 30.0%
!! GATTAATGGACTATTAGCTT+GGG - chr8.4:1993367-1993386 None:intergenic 30.0%
!! TGATTAATGGACTATTAGCT+TGG - chr8.4:1993368-1993387 None:intergenic 30.0%
!!! GTAAACCCTTTTTTTAATCG+GGG + chr8.4:1990512-1990531 MS.gene061199:intron 30.0%
!!! GTTCCTTTATTTTTTGTCCA+CGG - chr8.4:1991828-1991847 None:intergenic 30.0%
!!! TTCCTTTATTTTTTGTCCAC+GGG - chr8.4:1991827-1991846 None:intergenic 30.0%
AACCCGTGGACAAAAAATAA+AGG + chr8.4:1991822-1991841 MS.gene061199:intron 35.0%
AAGAATTCAGTAGCTGGATA+TGG + chr8.4:1992550-1992569 MS.gene061199:CDS 35.0%
AGAACTATGGAATCAAATGG+TGG + chr8.4:1993426-1993445 MS.gene061199:CDS 35.0%
AGGAAATGATGATAGAGAGA+GGG + chr8.4:1991612-1991631 MS.gene061199:CDS 35.0%
AGTGCATTTACAGATTCTTC+TGG + chr8.4:1992874-1992893 MS.gene061199:CDS 35.0%
ATTACTTGGGAATGTTGTAG+CGG + chr8.4:1993236-1993255 MS.gene061199:CDS 35.0%
CATTGCATTGTTTCCTCTAA+AGG - chr8.4:1993207-1993226 None:intergenic 35.0%
CATTTGCCCCGATTAAAAAA+AGG - chr8.4:1990521-1990540 None:intergenic 35.0%
CGACGATATTTGCAAAAATG+TGG - chr8.4:1992302-1992321 None:intergenic 35.0%
CTACAAAAGAATTCAGTAGC+TGG + chr8.4:1992544-1992563 MS.gene061199:CDS 35.0%
CTTAAGATTCTTCTTGATCC+AGG - chr8.4:1992592-1992611 None:intergenic 35.0%
CTTCTCATGTGCAAGTATAA+TGG + chr8.4:1990361-1990380 MS.gene061199:CDS 35.0%
GAAAACGCAGAAGAAGATAA+TGG + chr8.4:1991874-1991893 MS.gene061199:intron 35.0%
GAAACAATGCAATGTCAAGA+TGG + chr8.4:1993213-1993232 MS.gene061199:CDS 35.0%
GAAAGTAAGGATCCGAATAA+AGG + chr8.4:1991736-1991755 MS.gene061199:CDS 35.0%
GCAAATATCGTCGAAGTTAA+AGG + chr8.4:1992309-1992328 MS.gene061199:intron 35.0%
GTCACATAAACTCACTATCA+AGG - chr8.4:1991999-1992018 None:intergenic 35.0%
GTGACTTGATGAATGTTGAA+GGG + chr8.4:1992176-1992195 MS.gene061199:CDS 35.0%
GTGTCGAAGAAAAATCAGTT+GGG - chr8.4:1993170-1993189 None:intergenic 35.0%
TAATGGTAGAGAGAATGATG+AGG + chr8.4:1991891-1991910 MS.gene061199:intron 35.0%
TCAAACTTGTCTCAAACTGT+TGG + chr8.4:1990330-1990349 MS.gene061199:CDS 35.0%
TGAAAACTCGAGTGCTTTAA+AGG + chr8.4:1992765-1992784 MS.gene061199:CDS 35.0%
TGAATCACTACTTTGATGCT+TGG + chr8.4:1991308-1991327 MS.gene061199:intron 35.0%
TGATGTAAACATGCCAGAAA+TGG + chr8.4:1991416-1991435 MS.gene061199:CDS 35.0%
TGATTGAATGTGACATGTGT+CGG - chr8.4:1990660-1990679 None:intergenic 35.0%
TGCTAGCAATGAAAATGGTA+AGG + chr8.4:1990922-1990941 MS.gene061199:intron 35.0%
TGGAAATTAACCAGTTTCAC+CGG + chr8.4:1992803-1992822 MS.gene061199:CDS 35.0%
TGGAAGAGCTTAAGAACATA+TGG + chr8.4:1991689-1991708 MS.gene061199:CDS 35.0%
TGTGACTTGATGAATGTTGA+AGG + chr8.4:1992175-1992194 MS.gene061199:CDS 35.0%
TGTGATCATATAGTGTTGTC+AGG + chr8.4:1991592-1991611 MS.gene061199:intron 35.0%
TGTTCTTAAGCTCTTCCAAT+CGG - chr8.4:1991687-1991706 None:intergenic 35.0%
TTCAGAATAGTTGTTTGCCT+TGG + chr8.4:1993490-1993509 MS.gene061199:CDS 35.0%
TTCTTCAACTGTTATTGCAG+AGG + chr8.4:1992135-1992154 MS.gene061199:intron 35.0%
TTCTTCCTTCAACAATACCT+TGG + chr8.4:1993290-1993309 MS.gene061199:CDS 35.0%
! ACTGAATTCTTTTGTAGGTG+AGG - chr8.4:1992541-1992560 None:intergenic 35.0%
! CAGCTACTGAATTCTTTTGT+AGG - chr8.4:1992546-1992565 None:intergenic 35.0%
! GAAAACTCGAGTGCTTTAAA+GGG + chr8.4:1992766-1992785 MS.gene061199:CDS 35.0%
! TATTAGCTTGGGGTACATTA+TGG - chr8.4:1993356-1993375 None:intergenic 35.0%
!! AGAATCCAAGGTATTGTTGA+AGG - chr8.4:1993298-1993317 None:intergenic 35.0%
!! CAAATATACTTCTTTGGGGT+AGG - chr8.4:1990794-1990813 None:intergenic 35.0%
!! CAATGTCAAGATGGATTACT+TGG + chr8.4:1993222-1993241 MS.gene061199:CDS 35.0%
!! TAACATTAGCTCTGATGTCT+TGG - chr8.4:1992903-1992922 None:intergenic 35.0%
!!! TCGTCTTGTTTTGACATTTC+TGG + chr8.4:1990872-1990891 MS.gene061199:intron 35.0%
AAACAATGGCTGCTTGTAAC+TGG - chr8.4:1992737-1992756 None:intergenic 40.0%
AAAGGCTTCCTGCGTTATAA+AGG - chr8.4:1991268-1991287 None:intergenic 40.0%
AAATCCTCACTCAATGGAGA+AGG - chr8.4:1993081-1993100 None:intergenic 40.0%
AACAATGGCTGCTTGTAACT+GGG - chr8.4:1992736-1992755 None:intergenic 40.0%
AAGACATCTTCAACCTCCAA+AGG - chr8.4:1990262-1990281 None:intergenic 40.0%
AAGTTATAACCAAGACGTCG+TGG - chr8.4:1990594-1990613 None:intergenic 40.0%
AGAAGAAGCAAGAGAATCCA+AGG - chr8.4:1993310-1993329 None:intergenic 40.0%
AGCTTGAATCCATCCATTTC+TGG - chr8.4:1991432-1991451 None:intergenic 40.0%
AGGACTGGAAGCAAAGTTAT+AGG - chr8.4:1993061-1993080 None:intergenic 40.0%
ATAAATCACCCATCTGTTGG+AGG + chr8.4:1992991-1993010 MS.gene061199:CDS 40.0%
CAGGAAATGATGATAGAGAG+AGG + chr8.4:1991611-1991630 MS.gene061199:CDS 40.0%
CGTCATTGATGTTCCTTTAG+AGG + chr8.4:1993191-1993210 MS.gene061199:CDS 40.0%
CGTGTCGAAGAAAAATCAGT+TGG - chr8.4:1993171-1993190 None:intergenic 40.0%
CTTAAGTTCATGTTACCAGG+TGG - chr8.4:1992838-1992857 None:intergenic 40.0%
GAATGTTGAAGGGCTTACAA+GGG + chr8.4:1992186-1992205 MS.gene061199:CDS 40.0%
GAGCAATTGCTTGATGTAGA+TGG + chr8.4:1993462-1993481 MS.gene061199:CDS 40.0%
GATAAATGTTCCGGTGAAAC+TGG - chr8.4:1992816-1992835 None:intergenic 40.0%
GATTCTTCTTGATCCAGGTA+AGG - chr8.4:1992587-1992606 None:intergenic 40.0%
GCTAACAAGAATAAAACCCG+TGG + chr8.4:1991808-1991827 MS.gene061199:intron 40.0%
GGACTTAAGTTCATGTTACC+AGG - chr8.4:1992841-1992860 None:intergenic 40.0%
GTAAACATGCCAGAAATGGA+TGG + chr8.4:1991420-1991439 MS.gene061199:CDS 40.0%
GTGATGAAAGGAGTGATGAA+AGG + chr8.4:1991634-1991653 MS.gene061199:CDS 40.0%
TACCAGGTGGATAAATGTTC+CGG - chr8.4:1992825-1992844 None:intergenic 40.0%
TATAGGAACCACTTGTAGCA+GGG - chr8.4:1993044-1993063 None:intergenic 40.0%
TCATCATCCCAAACTAAACG+AGG - chr8.4:1991937-1991956 None:intergenic 40.0%
TCTACTCTAGCCTGTTTAGA+TGG - chr8.4:1992505-1992524 None:intergenic 40.0%
TCTCAAAAGGCCATCTAAAC+AGG + chr8.4:1992492-1992511 MS.gene061199:CDS 40.0%
TCTCTTGCTTCTTCTAATGC+AGG + chr8.4:1993315-1993334 MS.gene061199:CDS 40.0%
TCTTGTGAAACCAATCCGAT+TGG + chr8.4:1991669-1991688 MS.gene061199:CDS 40.0%
TGAATGTTGAAGGGCTTACA+AGG + chr8.4:1992185-1992204 MS.gene061199:CDS 40.0%
TGCATGAGAGGAGAATAGTA+CGG - chr8.4:1990455-1990474 None:intergenic 40.0%
TGGGAACAATAGACCTCATT+GGG + chr8.4:1991230-1991249 MS.gene061199:intron 40.0%
TGTGTGAGCTTACTGATGAA+GGG - chr8.4:1991486-1991505 None:intergenic 40.0%
TTAAGCTCTTCCAATCGGAT+TGG - chr8.4:1991682-1991701 None:intergenic 40.0%
TTATAGGAACCACTTGTAGC+AGG - chr8.4:1993045-1993064 None:intergenic 40.0%
TTGACTGTCCTTTATAACGC+AGG + chr8.4:1991257-1991276 MS.gene061199:intron 40.0%
TTGGAGGTTGAAGATGTCTT+CGG + chr8.4:1990262-1990281 MS.gene061199:CDS 40.0%
! AAAGACACTGCTAGTCTGTT+TGG + chr8.4:1991008-1991027 MS.gene061199:intron 40.0%
! AAATGTGGCTAGCCATTTAC+AGG + chr8.4:1992210-1992229 MS.gene061199:CDS 40.0%
! GAAGAAGCCTCGTTTAGTTT+GGG + chr8.4:1991927-1991946 MS.gene061199:intron 40.0%
! GACACATTAGTGTTGGAAGT+GGG + chr8.4:1991211-1991230 MS.gene061199:intron 40.0%
! TCTTTTAACCCTGCTACAAG+TGG + chr8.4:1993033-1993052 MS.gene061199:CDS 40.0%
! TGAAGCGCTAGAATTGTTGA+GGG + chr8.4:1991362-1991381 MS.gene061199:CDS 40.0%
! TGACACATTAGTGTTGGAAG+TGG + chr8.4:1991210-1991229 MS.gene061199:intron 40.0%
! TGAGCAAGTTGGACATGAAA+TGG + chr8.4:1991455-1991474 MS.gene061199:CDS 40.0%
! TGAGGCATCTGAGTGATTAA+TGG - chr8.4:1993381-1993400 None:intergenic 40.0%
! TTCGGAGAAGATTTTCCTGT+GGG + chr8.4:1990280-1990299 MS.gene061199:CDS 40.0%
! TTGAAGCGCTAGAATTGTTG+AGG + chr8.4:1991361-1991380 MS.gene061199:CDS 40.0%
! TTTCATGTCCAACTTGCTCA+AGG - chr8.4:1991455-1991474 None:intergenic 40.0%
!!! ATGTTTTGAGACTGAAGTGG+AGG - chr8.4:1992712-1992731 None:intergenic 40.0%
!!! GGAATGTTTTGAGACTGAAG+TGG - chr8.4:1992715-1992734 None:intergenic 40.0%
!!! TGTTTTGAGACTGAAGTGGA+GGG - chr8.4:1992711-1992730 None:intergenic 40.0%
ACTATTCTCCTCTCATGCAG+TGG + chr8.4:1990456-1990475 MS.gene061199:intron 45.0%
AGGGCTTACAAGGGAAAATG+TGG + chr8.4:1992195-1992214 MS.gene061199:CDS 45.0%
AGTTTACACCACTGCATGAG+AGG - chr8.4:1990467-1990486 None:intergenic 45.0%
CCATCTGGATTGAACTTGAG+AGG + chr8.4:1992664-1992683 MS.gene061199:CDS 45.0%
CCTAAATTGAGAACTGAGCG+AGG - chr8.4:1993142-1993161 None:intergenic 45.0%
CCTCGCTCAGTTCTCAATTT+AGG + chr8.4:1993139-1993158 MS.gene061199:CDS 45.0%
CCTCTCAAGTTCAATCCAGA+TGG - chr8.4:1992667-1992686 None:intergenic 45.0%
GACAGTCAATTCACCCAATG+AGG - chr8.4:1991246-1991265 None:intergenic 45.0%
GATAGAGAGAGGGTGATGAA+AGG + chr8.4:1991622-1991641 MS.gene061199:CDS 45.0%
GCATGAGAGGAGAATAGTAC+GGG - chr8.4:1990454-1990473 None:intergenic 45.0%
GGAAGAAGCCTCGTTTAGTT+TGG + chr8.4:1991926-1991945 MS.gene061199:intron 45.0%
GGAGATTTGTGTACCTTACC+TGG + chr8.4:1992571-1992590 MS.gene061199:CDS 45.0%
GTGGGAACAATAGACCTCAT+TGG + chr8.4:1991229-1991248 MS.gene061199:intron 45.0%
GTGTGTGAGCTTACTGATGA+AGG - chr8.4:1991487-1991506 None:intergenic 45.0%
TAATGCAGGCACTAGTTCCA+TGG + chr8.4:1993329-1993348 MS.gene061199:CDS 45.0%
TGAGCTTACTGATGAAGGGT+AGG - chr8.4:1991482-1991501 None:intergenic 45.0%
TTCAAGCTCCTTGAGCAAGT+TGG + chr8.4:1991444-1991463 MS.gene061199:CDS 45.0%
TTGGGCTGACACATTAGTGT+TGG + chr8.4:1991204-1991223 MS.gene061199:intron 45.0%
TTGGGGTACATTATGGACCA+TGG - chr8.4:1993349-1993368 None:intergenic 45.0%
! AATGTGACATGTGTCGGTGT+TGG - chr8.4:1990654-1990673 None:intergenic 45.0%
! CTTCGGAGAAGATTTTCCTG+TGG + chr8.4:1990279-1990298 MS.gene061199:CDS 45.0%
ATAGCAGGACCTCCAACAGA+TGG - chr8.4:1993003-1993022 None:intergenic 50.0%
ATCTGTTGGAGGTCCTGCTA+TGG + chr8.4:1993002-1993021 MS.gene061199:CDS 50.0%
ATGATGAGGAACGCTCGACT+CGG + chr8.4:1991905-1991924 MS.gene061199:intron 50.0%
CACCGGAACATTTATCCACC+TGG + chr8.4:1992820-1992839 MS.gene061199:CDS 50.0%
CAGTCCTTCTCCATTGAGTG+AGG + chr8.4:1993074-1993093 MS.gene061199:CDS 50.0%
CTCACTCAATGGAGAAGGAC+TGG - chr8.4:1993076-1993095 None:intergenic 50.0%
GAGTTTGAACCACGACGTCT+TGG + chr8.4:1990582-1990601 MS.gene061199:intron 50.0%
TACACGCAGCAGCAAAACGT+GGG + chr8.4:1993267-1993286 MS.gene061199:CDS 50.0%
TAGCAGGACCTCCAACAGAT+GGG - chr8.4:1993002-1993021 None:intergenic 50.0%
TCCTGTGGGAATGCATGTTC+TGG + chr8.4:1990294-1990313 MS.gene061199:CDS 50.0%
TTACACGCAGCAGCAAAACG+TGG + chr8.4:1993266-1993285 MS.gene061199:CDS 50.0%
!! AAAGGGTGTTCGAACGTCAG+TGG + chr8.4:1992783-1992802 MS.gene061199:CDS 50.0%
!! GGCTTTGGAGAATCCTTTGG+AGG + chr8.4:1990246-1990265 MS.gene061199:CDS 50.0%
GCCAGAACATGCATTCCCAC+AGG - chr8.4:1990298-1990317 None:intergenic 55.0%
! TGTCGGCTGAACGCACCATC+TGG + chr8.4:1992649-1992668 MS.gene061199:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 1990244 1993524 1990244 ID=MS.gene061199
chr8.4 mRNA 1990244 1993524 1990244 ID=MS.gene061199.t1;Parent=MS.gene061199
chr8.4 exon 1990244 1990382 1990244 ID=MS.gene061199.t1.exon1;Parent=MS.gene061199.t1
chr8.4 CDS 1990244 1990382 1990244 ID=cds.MS.gene061199.t1;Parent=MS.gene061199.t1
chr8.4 exon 1991340 1991492 1991340 ID=MS.gene061199.t1.exon2;Parent=MS.gene061199.t1
chr8.4 CDS 1991340 1991492 1991340 ID=cds.MS.gene061199.t1;Parent=MS.gene061199.t1
chr8.4 exon 1991605 1991757 1991605 ID=MS.gene061199.t1.exon3;Parent=MS.gene061199.t1
chr8.4 CDS 1991605 1991757 1991605 ID=cds.MS.gene061199.t1;Parent=MS.gene061199.t1
chr8.4 exon 1991944 1992003 1991944 ID=MS.gene061199.t1.exon4;Parent=MS.gene061199.t1
chr8.4 CDS 1991944 1992003 1991944 ID=cds.MS.gene061199.t1;Parent=MS.gene061199.t1
chr8.4 exon 1992155 1992231 1992155 ID=MS.gene061199.t1.exon5;Parent=MS.gene061199.t1
chr8.4 CDS 1992155 1992231 1992155 ID=cds.MS.gene061199.t1;Parent=MS.gene061199.t1
chr8.4 exon 1992478 1993524 1992478 ID=MS.gene061199.t1.exon6;Parent=MS.gene061199.t1
chr8.4 CDS 1992478 1993524 1992478 ID=cds.MS.gene061199.t1;Parent=MS.gene061199.t1
Gene Sequence

>MS.gene061199

ATGGCTTTGGAGAATCCTTTGGAGGTTGAAGATGTCTTCGGAGAAGATTTTCCTGTGGGAATGCATGTTCTGGCTGTTGACGACGATCAAACTTGTCTCAAACTGTTGGAAAAGTTTCTTCTCATGTGCAAGTATAATGGTTCGTTTCCATTTTTTCTTCTTTCTTTCAGTCAATTCATTTCTGCATTGATGATTCTTTGATGTTTTCCCGTACTATTCTCCTCTCATGCAGTGGTGTAAACTTATAATTGGAACTTTCATTGATATAGTAAACCCTTTTTTTAATCGGGGCAAATGTTACTTGTTAGTAATTTAGTGATGATTATATTAGTTTTTCAGAGTTTGAACCACGACGTCTTGGTTATAACTTATAAGCTTTGTAGTCGGTGCTGAGTGTGTGTTAGTATCCAACACCGACACATGTCACATTCAATCACTTGTTTTCTTAAAATATTTTCGATGTAATCTTGGCTACCCCAATTTTTTGTTTATTTCGGGTATAAATAAGTATACTTTTATGTTCAGCCACCCTAATAATATATGTTGACCTACCCCAAAGAAGTATATTTGCCAAAAAATTAGTAGAAACATAAAAGTTAAAATATTATTATTTAATTGATAATATTATTCGTCTTGTTTTGACATTTCTGGTCTTTACAACAAATATAGAGAGAACTTTGCTAGCAATGAAAATGGTAAGGTAAAAGTCGAAAATAAAAATAGAGGATGAGTTTCTTGCAAATAATTCAGTTGTTTTGATTGGAAAAGACACTGCTAGTCTGTTTGGTACAAATTATGAATGAATATGATTTTTTGAAGTGTTGCTGAATTCAACTTTCCTAAATACTGTTTTCTCATTTTCTATTATTCTGTGCTGCTCTAATTTACATTTAAAATAAGTAAAAATTATTGCAAATTATTGTAATGTAATTGTATATGTACATAAGATCACTACTTTTATTGGGCTGACACATTAGTGTTGGAAGTGGGAACAATAGACCTCATTGGGTGAATTGACTGTCCTTTATAACGCAGGAAGCCTTTTTATTTTAAATGTCTCTCACTGAATCACTACTTTGATGCTTGGTTGTTGCAGTTACTACAACTACGAAGTCAGTTGAAGCGCTAGAATTGTTGAGGGAAAAGAGAGAGATGTTTGACATTGTAATTAGTGATGTAAACATGCCAGAAATGGATGGATTCAAGCTCCTTGAGCAAGTTGGACATGAAATGGACCTACCCTTCATCAGTAAGCTCACACACCTTTGTTTTAGCATGTGTATTATTCAGATAACTAAGATTCACTTCTTATATTCATATGGTATCTTTAAGTGATGATTTCTTTTTGTGTGATCATATAGTGTTGTCAGGAAATGATGATAGAGAGAGGGTGATGAAAGGAGTGATGAAAGGCGCTTGCGACTATCTTGTGAAACCAATCCGATTGGAAGAGCTTAAGAACATATGGCAACATGTAGTGAGAAAGAAGATTGAAAGTAAGGATCCGAATAAAGGTATCATTACTGATGGGATATGTAATCAAGACACCTCATCTGAAAATATTGCTAACAAGAATAAAACCCGTGGACAAAAAATAAAGGAACAAAGTGAGGATGAAGAAGACGAAGAAGAAAACGCAGAAGAAGATAATGGTAGAGAGAATGATGAGGAACGCTCGACTCGGAAGAAGCCTCGTTTAGTTTGGGATGATGAATTGCATAAAAAATTTGTATCTATAGTTAGTCAACTCGGCCTTGATAGTGAGTTTATGTGACAATCATTTTTTTTTTTGTTACTTTACTAATTTGATTGGCTTTAAAACATGGAACCTTAACAAGACCATTTGATGATTGAAACTTACATATGGATATTATTGTTCCTAACATGTACTTTCTTCAACTGTTATTGCAGAGGCTTATCCTAAGAAAATATGTGACTTGATGAATGTTGAAGGGCTTACAAGGGAAAATGTGGCTAGCCATTTACAGGTACAAACTATAATTTCCCCTTTTGTCAAAATATTTCATACTAGTTTATCATTAAAATATTTGATCACCACATTTTTGCAAATATCGTCGAAGTTAAAGGGGATAATTTACTTTTAAACATATGCTTGTATGCAAATATGGTGTGAAAGTTTTTTATATCCATTATTCGTTGAAATTTCAACAGTCTCTTTTATGAAACATAAGTGGGAAAACTGCATGTAAGCTCGTTCTTGTTTTTTATTGCAGAAATACAAACTTAGTCTCAAAAGGCCATCTAAACAGGCTAGAGTAGATGCTGCATTAGATCCTCACCTACAAAAGAATTCAGTAGCTGGATATGGAGATTTGTGTACCTTACCTGGATCAAGAAGAATCTTAAGCAGCACATTGCCAACATATGCATCTAGTAACATGTATTGTCGGCTGAACGCACCATCTGGATTGAACTTGAGAGGAATGAGTTCTTCTGCATTAGTCCCTCCACTTCAGTCTCAAAACATTCCCAGTTACAAGCAGCCATTGTTTTTTGCAAGTGAAAACTCGAGTGCTTTAAAGGGTGTTCGAACGTCAGTGGAAATTAACCAGTTTCACCGGAACATTTATCCACCTGGTAACATGAACTTAAGTCCAATTGATGATTCAAGTGCATTTACAGATTCTTCTGGCTTCCAAGACATCAGAGCTAATGTTAATAATGCAAATAGTTTTCTTTCTTGCATCTCAAGCAATCACCTACAAACACATAATTCAAGAGCATTTATAAATCACCCATCTGTTGGAGGTCCTGCTATGGAACAAAAATCTTTTAACCCTGCTACAAGTGGTTCCTATAACTTTGCTTCCAGTCCTTCTCCATTGAGTGAGGATTTTAATAATGATCAAATATCTCACAATAGCTTAAAGTTTGCCTCGCTCAGTTCTCAATTTAGGAAAAGCCCAACTGATTTTTCTTCGACACGCGTCATTGATGTTCCTTTAGAGGAAACAATGCAATGTCAAGATGGATTACTTGGGAATGTTGTAGCGGCTTCATGTTACACGCAGCAGCAAAACGTGGGTTCTTCCTTCAACAATACCTTGGATTCTCTTGCTTCTTCTAATGCAGGCACTAGTTCCATGGTCCATAATGTACCCCAAGCTAATAGTCCATTAATCACTCAGATGCCTCAAGTTGAGAAATTTTATTCAGATGAAAGAACTATGGAATCAAATGGTGGTGATTGCTTTTTTGAGCAATTGCTTGATGTAGATGGATTCGTTCAGAATAGTTGTTTGCCTTGGATGATACAATAA
Protein sequence

>MS.gene061199.t1

MALENPLEVEDVFGEDFPVGMHVLAVDDDQTCLKLLEKFLLMCKYNVTTTTKSVEALELLREKREMFDIVISDVNMPEMDGFKLLEQVGHEMDLPFIMLSGNDDRERVMKGVMKGACDYLVKPIRLEELKNIWQHVVRKKIESKDPNKVWDDELHKKFVSIVSQLGLDKAYPKKICDLMNVEGLTRENVASHLQKYKLSLKRPSKQARVDAALDPHLQKNSVAGYGDLCTLPGSRRILSSTLPTYASSNMYCRLNAPSGLNLRGMSSSALVPPLQSQNIPSYKQPLFFASENSSALKGVRTSVEINQFHRNIYPPGNMNLSPIDDSSAFTDSSGFQDIRANVNNANSFLSCISSNHLQTHNSRAFINHPSVGGPAMEQKSFNPATSGSYNFASSPSPLSEDFNNDQISHNSLKFASLSSQFRKSPTDFSSTRVIDVPLEETMQCQDGLLGNVVAASCYTQQQNVGSSFNNTLDSLASSNAGTSSMVHNVPQANSPLITQMPQVEKFYSDERTMESNGGDCFFEQLLDVDGFVQNSCLPWMIQ