AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061319

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061319.t1 MTR_4g049790 72.655 629 106 5 1 629 1 563 0.0 823
MS.gene061319.t1 MTR_4g047580 67.608 602 133 8 28 629 1 540 0.0 733
MS.gene061319.t1 MTR_4g049760 70.775 568 113 5 33 600 8 522 0.0 710
MS.gene061319.t1 MTR_2g088000 49.100 389 132 10 263 605 222 590 4.24e-93 299
MS.gene061319.t1 MTR_2g023930 36.746 547 235 15 60 578 67 530 3.31e-83 272
MS.gene061319.t1 MTR_4g132430 55.752 226 90 5 344 565 105 324 1.81e-71 235
MS.gene061319.t1 MTR_4g132430 55.752 226 90 5 344 565 239 458 9.28e-71 237
MS.gene061319.t1 MTR_3g053330 48.739 238 92 5 346 577 231 444 4.98e-64 218
MS.gene061319.t1 MTR_7g110720 46.245 253 102 5 351 602 174 393 1.03e-56 197
MS.gene061319.t1 MTR_7g110720 46.245 253 102 5 351 602 201 420 1.08e-56 198
MS.gene061319.t1 MTR_2g045360 53.892 167 68 2 351 510 196 360 4.19e-48 174
MS.gene061319.t1 MTR_7g009730 51.613 124 54 1 323 440 170 293 3.23e-37 147
MS.gene061319.t1 MTR_4g094412 37.548 261 143 5 351 592 228 487 3.15e-36 142
MS.gene061319.t1 MTR_4g094412 37.548 261 143 5 351 592 219 478 5.38e-36 141
MS.gene061319.t1 MTR_4g107970 34.604 341 147 8 327 591 198 538 1.80e-31 130
MS.gene061319.t1 MTR_4g107970 35.786 299 134 5 351 591 163 461 2.10e-31 129
MS.gene061319.t1 MTR_5g042920 50.515 97 43 2 336 428 286 381 3.09e-24 106
MS.gene061319.t1 MTR_5g042920 36.111 108 65 3 367 474 152 255 1.15e-11 67.8
MS.gene061319.t1 MTR_5g094430 55.000 80 35 1 349 428 213 291 3.91e-24 105
MS.gene061319.t1 MTR_5g094430 55.000 80 35 1 349 428 235 313 3.99e-24 105
MS.gene061319.t1 MTR_7g011820 48.454 97 49 1 342 438 386 481 4.24e-24 107
MS.gene061319.t1 MTR_3g056100 43.119 109 58 2 339 444 231 338 5.29e-24 105
MS.gene061319.t1 MTR_3g056100 43.119 109 58 2 339 444 244 351 5.37e-24 105
MS.gene061319.t1 MTR_3g056100 43.119 109 58 2 339 444 388 495 7.35e-24 106
MS.gene061319.t1 MTR_3g056100 43.119 109 58 2 339 444 375 482 7.56e-24 106
MS.gene061319.t1 MTR_5g094430 55.000 80 35 1 349 428 347 425 1.17e-23 105
MS.gene061319.t1 MTR_4g007060 35.099 151 74 4 343 493 105 231 1.98e-23 101
MS.gene061319.t1 MTR_4g007060 35.099 151 74 4 343 493 139 265 3.16e-23 101
MS.gene061319.t1 MTR_5g074400 48.387 93 47 1 336 428 390 481 4.98e-23 103
MS.gene061319.t1 MTR_5g074400 48.333 60 29 2 367 426 244 301 6.54e-11 65.9
MS.gene061319.t1 MTR_3g031220 46.316 95 50 1 333 427 332 425 1.10e-22 102
MS.gene061319.t1 MTR_3g031220 41.379 87 45 3 344 430 180 260 9.70e-13 71.2
MS.gene061319.t1 MTR_4g082580 51.852 81 38 1 348 428 260 339 1.27e-22 100
MS.gene061319.t1 MTR_3g009470 54.321 81 36 1 348 428 477 556 1.37e-22 103
MS.gene061319.t1 MTR_7g071120 54.321 81 36 1 348 428 306 385 5.19e-22 100
MS.gene061319.t1 MTR_4g013260 53.165 79 36 1 348 426 318 395 7.83e-22 99.8
MS.gene061319.t1 MTR_4g013260 41.860 86 43 3 350 433 170 250 6.64e-12 68.6
MS.gene061319.t1 MTR_7g080000 43.478 115 62 2 316 428 380 493 1.01e-21 100
MS.gene061319.t1 MTR_5g097490 53.012 83 37 2 347 428 232 313 1.14e-21 98.2
MS.gene061319.t1 MTR_4g094588 42.593 108 61 1 353 460 165 271 2.65e-21 95.9
MS.gene061319.t1 MTR_7g080020 50.617 81 39 1 348 428 475 554 3.81e-21 98.6
MS.gene061319.t1 MTR_3g106060 49.451 91 39 2 365 450 137 225 5.38e-21 94.4
MS.gene061319.t1 MTR_5g044695 66.038 53 18 0 344 396 55 107 5.78e-21 88.6
MS.gene061319.t1 MTR_7g109800 45.745 94 46 2 351 444 130 218 5.84e-21 94.4
MS.gene061319.t1 MTR_1g013790 36.290 124 64 4 317 426 39 161 1.46e-20 92.0
MS.gene061319.t1 MTR_2g105060 48.718 78 38 1 352 427 153 230 1.52e-20 93.2
MS.gene061319.t1 MTR_2g105060 48.718 78 38 1 352 427 78 155 1.68e-20 91.7
MS.gene061319.t1 MTR_1g013760 44.565 92 51 0 351 442 125 216 4.63e-20 91.3
MS.gene061319.t1 MTR_6g038890 66.038 53 18 0 344 396 166 218 6.52e-20 89.4
MS.gene061319.t1 MTR_7g100510 43.878 98 44 2 329 426 334 420 7.67e-20 93.2
MS.gene061319.t1 MTR_7g100510 46.479 71 36 2 367 437 190 258 6.27e-12 68.6
MS.gene061319.t1 MTR_1g015140 51.351 74 35 1 351 424 87 159 1.66e-19 86.3
MS.gene061319.t1 MTR_1g009613 56.716 67 29 0 367 433 263 329 1.80e-19 90.5
MS.gene061319.t1 MTR_8g096730 37.143 105 65 1 325 429 29 132 2.42e-19 85.5
MS.gene061319.t1 MTR_2g033820 48.718 78 39 1 353 430 190 266 3.54e-19 90.5
MS.gene061319.t1 MTR_7g079010 51.389 72 34 1 353 424 128 198 4.25e-19 87.0
MS.gene061319.t1 MTR_4g094908 56.452 62 27 0 367 428 267 328 6.07e-19 89.0
MS.gene061319.t1 MTR_3g104750 39.252 107 57 2 318 424 63 161 6.08e-19 84.7
MS.gene061319.t1 MTR_7g028415 52.857 70 32 1 356 425 83 151 7.30e-19 84.7
MS.gene061319.t1 MTR_7g028710 52.857 70 32 1 356 425 83 151 7.30e-19 84.7
MS.gene061319.t1 MTR_8g087000 53.968 63 29 0 366 428 77 139 1.08e-18 86.3
MS.gene061319.t1 MTR_8g087000 53.968 63 29 0 366 428 76 138 1.19e-18 86.3
MS.gene061319.t1 MTR_1g086790 47.436 78 40 1 353 430 145 221 2.16e-18 87.0
MS.gene061319.t1 MTR_2g032030 43.011 93 46 1 351 436 245 337 2.44e-18 89.0
MS.gene061319.t1 MTR_5g016610 56.667 60 26 0 366 425 199 258 2.83e-18 85.9
MS.gene061319.t1 MTR_2g024350 34.043 141 81 3 361 490 3 142 3.38e-18 84.3
MS.gene061319.t1 MTR_2g032030 43.011 93 46 1 351 436 245 337 3.70e-18 87.8
MS.gene061319.t1 MTR_7g109600 42.857 91 49 1 362 452 49 136 3.83e-18 85.1
MS.gene061319.t1 MTR_1g070455 57.895 57 24 0 367 423 294 350 8.00e-18 85.9
MS.gene061319.t1 MTR_5g091390 47.436 78 40 1 353 430 144 220 8.08e-18 85.1
MS.gene061319.t1 MTR_2g083870 45.570 79 43 0 357 435 71 149 9.52e-18 84.0
MS.gene061319.t1 MTR_2g016770 51.562 64 31 0 363 426 32 95 1.51e-17 82.4
MS.gene061319.t1 MTR_8g007270 49.367 79 39 1 345 423 133 210 1.52e-17 82.4
MS.gene061319.t1 MTR_2g016770 52.459 61 29 0 366 426 27 87 2.15e-17 81.6
MS.gene061319.t1 MTR_4g130900 47.500 80 41 1 351 430 344 422 2.47e-17 85.9
MS.gene061319.t1 MTR_7g079040 48.000 75 39 0 362 436 150 224 5.46e-17 82.8
MS.gene061319.t1 MTR_8g092010 45.455 77 41 1 353 429 95 170 5.48e-17 79.7
MS.gene061319.t1 MTR_4g122530 46.053 76 40 1 352 427 194 268 8.45e-17 83.6
MS.gene061319.t1 MTR_3g095080 43.478 92 50 2 353 444 131 220 9.57e-17 80.1
MS.gene061319.t1 MTR_2g024380 44.186 86 46 1 352 437 239 322 1.29e-16 83.2
MS.gene061319.t1 MTR_3g095080 43.478 92 50 2 353 444 155 244 1.45e-16 80.1
MS.gene061319.t1 MTR_8g098945 45.833 72 39 0 365 436 128 199 1.55e-16 80.5
MS.gene061319.t1 MTR_1g077780 44.444 81 44 1 348 428 44 123 2.94e-16 76.6
MS.gene061319.t1 MTR_8g028565 46.667 75 40 0 362 436 151 225 3.12e-16 80.9
MS.gene061319.t1 MTR_4g127040 51.667 60 29 0 364 423 243 302 3.32e-16 80.5
MS.gene061319.t1 MTR_1g099600 43.956 91 49 1 366 456 185 273 4.44e-16 80.1
MS.gene061319.t1 MTR_5g043880 47.436 78 40 1 346 423 104 180 6.16e-16 79.0
MS.gene061319.t1 MTR_3g095040 46.667 75 39 1 353 427 105 178 2.62e-15 75.5
MS.gene061319.t1 MTR_8g092140 39.000 100 59 2 325 423 118 216 3.04e-15 75.9
MS.gene061319.t1 MTR_1g078260 48.611 72 35 2 353 423 234 304 2.95e-14 75.9
MS.gene061319.t1 MTR_6g086555 47.500 80 40 2 353 431 193 271 6.77e-14 74.3
MS.gene061319.t1 MTR_3g031220 37.168 113 63 4 320 430 154 260 3.71e-13 72.0
MS.gene061319.t1 MTR_3g108960 42.029 69 39 1 355 423 304 371 8.31e-13 70.9
MS.gene061319.t1 MTR_4g007060 40.984 61 36 0 343 403 139 199 2.99e-12 67.4
MS.gene061319.t1 MTR_0117s0040 42.029 69 39 1 355 423 64 131 3.68e-12 67.0
MS.gene061319.t1 MTR_5g097500 31.959 97 65 1 332 428 196 291 5.50e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061319.t1 AT4G04450 39.773 528 226 15 65 577 53 503 2.22e-86 280
MS.gene061319.t1 AT4G04450 40.710 479 199 14 114 577 1 409 9.29e-81 262
MS.gene061319.t1 AT1G62300 45.161 341 153 11 282 593 217 552 2.27e-69 235
MS.gene061319.t1 AT4G22070 55.510 245 91 6 345 577 276 514 3.91e-62 215
MS.gene061319.t1 AT4G22070 55.510 245 91 6 345 577 276 514 3.91e-62 215
MS.gene061319.t1 AT4G22070 55.510 245 91 6 345 577 276 514 3.91e-62 215
MS.gene061319.t1 AT4G22070 55.510 245 91 6 345 577 276 514 3.91e-62 215
MS.gene061319.t1 AT4G01720 37.121 396 205 9 231 606 104 475 1.29e-47 174
MS.gene061319.t1 AT1G68150 63.918 97 35 0 351 447 220 316 1.19e-39 149
MS.gene061319.t1 AT5G15130 36.531 271 121 7 346 578 165 422 5.88e-38 147
MS.gene061319.t1 AT5G15130 36.531 271 121 7 346 578 207 464 6.60e-38 148
MS.gene061319.t1 AT1G69810 43.716 183 92 5 320 501 103 275 7.03e-36 138
MS.gene061319.t1 AT1G69810 45.783 166 83 4 337 501 174 333 8.73e-36 139
MS.gene061319.t1 AT1G18860 31.016 374 194 11 139 495 48 374 3.78e-34 137
MS.gene061319.t1 AT4G26640 52.500 80 37 1 349 428 188 266 6.88e-24 104
MS.gene061319.t1 AT4G26640 52.500 80 37 1 349 428 188 266 6.88e-24 104
MS.gene061319.t1 AT4G26640 52.500 80 37 1 349 428 188 266 6.88e-24 104
MS.gene061319.t1 AT4G26640 52.500 80 37 1 349 428 188 266 6.88e-24 104
MS.gene061319.t1 AT1G13960 55.696 79 34 1 348 426 238 315 7.58e-24 103
MS.gene061319.t1 AT1G13960 51.562 64 29 2 367 430 77 138 2.90e-12 68.9
MS.gene061319.t1 AT1G13960 55.696 79 34 1 348 426 391 468 7.92e-24 105
MS.gene061319.t1 AT1G13960 51.562 64 29 2 367 430 230 291 2.11e-12 70.5
MS.gene061319.t1 AT2G38470 54.321 81 36 1 348 428 344 423 1.16e-23 105
MS.gene061319.t1 AT2G38470 48.333 60 29 2 367 426 185 242 3.94e-11 66.2
MS.gene061319.t1 AT4G26640 52.500 80 37 1 349 428 292 370 1.72e-23 104
MS.gene061319.t1 AT4G26640 52.500 80 37 1 349 428 324 402 1.75e-23 105
MS.gene061319.t1 AT4G26640 52.500 80 37 1 349 428 364 442 2.30e-23 105
MS.gene061319.t1 AT2G03340 43.966 116 55 2 313 428 371 476 2.61e-23 104
MS.gene061319.t1 AT2G03340 53.333 60 26 2 367 426 251 308 2.94e-12 69.7
MS.gene061319.t1 AT2G25000 32.386 176 106 3 266 429 14 188 4.64e-22 96.3
MS.gene061319.t1 AT5G56270 53.086 81 37 1 348 428 469 548 6.64e-22 100
MS.gene061319.t1 AT4G31800 48.352 91 42 1 345 430 85 175 7.14e-22 95.5
MS.gene061319.t1 AT4G31800 48.352 91 42 1 345 430 150 240 8.70e-22 97.1
MS.gene061319.t1 AT2G04880 45.055 91 49 1 336 426 253 342 9.13e-22 99.4
MS.gene061319.t1 AT2G04880 50.725 69 30 3 362 429 107 172 3.69e-12 69.3
MS.gene061319.t1 AT3G01080 48.837 86 43 1 341 426 319 403 1.19e-21 99.0
MS.gene061319.t1 AT2G04880 45.055 91 49 1 336 426 277 366 1.23e-21 99.0
MS.gene061319.t1 AT2G04880 50.725 69 30 3 362 429 107 172 3.95e-12 69.3
MS.gene061319.t1 AT3G01080 48.837 86 43 1 341 426 281 365 1.50e-21 98.2
MS.gene061319.t1 AT5G41570 46.465 99 48 2 353 451 85 178 1.82e-21 92.4
MS.gene061319.t1 AT5G07100 48.235 85 43 1 346 430 121 204 1.89e-21 93.6
MS.gene061319.t1 AT5G07100 48.235 85 43 1 346 430 121 204 1.89e-21 93.6
MS.gene061319.t1 AT5G07100 48.235 85 43 1 346 430 121 204 1.89e-21 93.6
MS.gene061319.t1 AT5G07100 48.235 85 43 1 346 430 121 204 1.89e-21 93.6
MS.gene061319.t1 AT5G07100 48.235 85 43 1 346 430 214 297 2.77e-21 95.5
MS.gene061319.t1 AT1G80840 47.436 78 39 1 352 427 130 207 2.92e-21 95.1
MS.gene061319.t1 AT2G25000 34.000 150 97 2 281 429 18 166 3.76e-21 93.2
MS.gene061319.t1 AT2G25000 34.000 150 97 2 281 429 18 166 3.76e-21 93.2
MS.gene061319.t1 AT1G64000 54.545 77 34 1 348 424 96 171 4.16e-21 92.0
MS.gene061319.t1 AT1G29860 46.809 94 44 2 351 444 121 208 5.47e-21 94.0
MS.gene061319.t1 AT2G25000 34.000 150 97 2 281 429 61 209 6.23e-21 93.6
MS.gene061319.t1 AT5G26170 54.167 72 32 1 352 423 99 169 1.17e-20 90.1
MS.gene061319.t1 AT4G18170 44.000 100 55 1 353 452 159 257 1.57e-20 93.6
MS.gene061319.t1 AT5G46350 46.316 95 50 1 353 447 170 263 2.19e-20 93.2
MS.gene061319.t1 AT2G30250 47.674 86 44 1 345 430 307 391 3.35e-20 93.6
MS.gene061319.t1 AT1G29280 51.429 70 34 0 357 426 65 134 1.55e-19 89.4
MS.gene061319.t1 AT2G23320 59.322 59 24 0 367 425 241 299 1.72e-19 90.5
MS.gene061319.t1 AT4G30935 38.281 128 69 3 334 456 299 421 2.47e-19 91.7
MS.gene061319.t1 AT5G49520 46.250 80 42 1 351 430 206 284 2.78e-19 90.9
MS.gene061319.t1 AT5G13080 52.055 73 34 1 353 425 54 125 3.72e-19 84.7
MS.gene061319.t1 AT2G30590 57.812 64 26 1 367 429 314 377 5.50e-19 89.7
MS.gene061319.t1 AT1G55600 48.684 76 38 1 353 428 294 368 1.51e-18 89.4
MS.gene061319.t1 AT4G26440 51.316 76 36 1 353 428 359 433 1.68e-18 89.7
MS.gene061319.t1 AT2G46130 47.619 84 42 2 342 424 5 87 2.70e-18 81.3
MS.gene061319.t1 AT3G58710 46.575 73 39 0 357 429 62 134 3.19e-18 85.5
MS.gene061319.t1 AT3G58710 46.575 73 39 0 357 429 61 133 3.48e-18 85.5
MS.gene061319.t1 AT5G64810 47.872 94 45 2 333 423 74 166 4.77e-18 83.2
MS.gene061319.t1 AT5G28650 60.377 53 21 0 371 423 267 319 5.15e-18 86.3
MS.gene061319.t1 AT4G24240 54.839 62 28 0 364 425 279 340 5.43e-18 86.3
MS.gene061319.t1 AT2G34830 44.086 93 45 1 348 433 190 282 1.24e-17 86.3
MS.gene061319.t1 AT3G01970 51.389 72 34 1 352 423 51 121 1.28e-17 80.5
MS.gene061319.t1 AT5G52830 46.753 77 41 0 360 436 159 235 1.32e-17 85.1
MS.gene061319.t1 AT1G30650 44.086 93 45 1 348 433 192 284 1.38e-17 86.3
MS.gene061319.t1 AT2G34830 44.086 93 45 1 348 433 199 291 1.44e-17 86.3
MS.gene061319.t1 AT3G04670 60.377 53 21 0 371 423 267 319 1.45e-17 84.7
MS.gene061319.t1 AT2G24570 59.649 57 23 0 367 423 244 300 1.47e-17 84.7
MS.gene061319.t1 AT2G47260 45.783 83 44 1 351 433 159 240 2.12e-17 84.3
MS.gene061319.t1 AT3G62340 52.703 74 34 1 353 426 105 177 2.31e-17 83.2
MS.gene061319.t1 AT2G44745 46.835 79 41 1 345 423 124 201 2.64e-17 81.6
MS.gene061319.t1 AT5G45050 28.111 217 141 5 316 529 997 1201 3.80e-17 86.3
MS.gene061319.t1 AT4G31550 57.895 57 24 0 367 423 246 302 4.16e-17 83.2
MS.gene061319.t1 AT4G31550 57.895 57 24 0 367 423 247 303 4.18e-17 83.2
MS.gene061319.t1 AT5G45050 28.111 217 141 5 316 529 1136 1340 4.30e-17 86.3
MS.gene061319.t1 AT5G45050 28.111 217 141 5 316 529 1108 1312 4.46e-17 86.3
MS.gene061319.t1 AT4G01250 46.429 84 43 1 353 436 117 198 4.96e-17 82.8
MS.gene061319.t1 AT4G39410 52.113 71 33 1 353 423 210 279 4.24e-16 80.1
MS.gene061319.t1 AT4G23550 44.737 76 42 0 361 436 135 210 4.37e-16 80.1
MS.gene061319.t1 AT1G69310 44.000 75 41 1 349 423 130 203 1.51e-15 78.2
MS.gene061319.t1 AT1G69310 44.000 75 41 1 349 423 130 203 1.51e-15 78.2
MS.gene061319.t1 AT1G69310 44.000 75 41 1 349 423 130 203 1.51e-15 78.2
MS.gene061319.t1 AT2G37260 53.846 65 29 1 361 425 264 327 2.46e-15 78.2
MS.gene061319.t1 AT2G37260 48.529 68 28 3 367 429 86 151 1.08e-11 67.4
MS.gene061319.t1 AT2G37260 53.846 65 29 1 361 425 262 325 2.48e-15 78.2
MS.gene061319.t1 AT2G37260 48.529 68 28 3 367 429 84 149 1.09e-11 67.0
MS.gene061319.t1 AT2G37260 53.846 65 29 1 361 425 344 407 3.65e-15 78.6
MS.gene061319.t1 AT2G37260 48.529 68 28 3 367 429 166 231 1.15e-11 67.8
MS.gene061319.t1 AT2G46130 45.238 84 40 3 342 424 5 83 6.77e-14 68.6
MS.gene061319.t1 AT2G21900 42.029 69 39 1 361 429 104 171 2.09e-13 70.1
MS.gene061319.t1 AT5G45260 43.421 76 41 1 367 440 1209 1284 2.03e-12 71.2
MS.gene061319.t1 AT4G31800 48.438 64 28 1 345 403 150 213 5.42e-12 66.6

Find 111 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAACTGATCTTGACTTCT+AGG 0.104418 4.2:-51957827 None:intergenic
TACTGATACACCATCTTTAT+TGG 0.104474 4.2:+51955822 MS.gene061319:CDS
ATTGGCTACTGATAAGTTTA+AGG 0.186081 4.2:+51957646 MS.gene061319:CDS
GACAAAGCTGCAGGAATATT+TGG 0.214770 4.2:-51959338 None:intergenic
GTTCTCTTCTCTTCTGAATT+AGG 0.215204 4.2:-51956090 None:intergenic
GCTGCTATTACATCCATTAT+TGG 0.245842 4.2:+51959362 MS.gene061319:CDS
GATAAGTTTAAGGAAGTTAA+AGG 0.251785 4.2:+51957656 MS.gene061319:CDS
ACTGAGGCCAAGGAAATTTA+TGG 0.255959 4.2:+51957496 MS.gene061319:CDS
GCGTTCCTTTGTGGAGTATT+TGG 0.276464 4.2:-51959122 None:intergenic
ACTGTTTGAGCAGTAATATT+TGG 0.280261 4.2:-51959293 None:intergenic
CGTAAGGGTAAGAAAGTTAA+AGG 0.311737 4.2:+51957416 MS.gene061319:CDS
TCCCTTTCATAGTCAATATT+AGG 0.325926 4.2:+51955668 MS.gene061319:CDS
AGAGCTTGAACAAACGAAAA+TGG 0.333537 4.2:+51957307 MS.gene061319:CDS
GATAAGGTTAAGGAAGTTAA+AGG 0.335137 4.2:+51957536 MS.gene061319:CDS
AGCTGACCCTTCACAAATAT+TGG 0.335185 4.2:+51959265 MS.gene061319:CDS
AACTGATCTGCGTTCCTTTG+TGG 0.348394 4.2:-51959131 None:intergenic
GGGAAAGAGTTGTCATTAAC+TGG 0.356037 4.2:-51955577 None:intergenic
AGAAGAAACTTACATGCTTT+CGG 0.356423 4.2:-51958190 None:intergenic
GGATGTCAATGGAGAAAGTA+TGG 0.356478 4.2:+51958095 MS.gene061319:CDS
CTACTAGTATTGATAGTAAT+AGG 0.368547 4.2:-51956036 None:intergenic
GCCATTTCTTTGGCTGCTGC+AGG 0.373631 4.2:-51958927 None:intergenic
AGAGAAGAGAACTAAGAGTA+TGG 0.375742 4.2:+51956101 MS.gene061319:CDS
CTGCAGCGATAATAAGCTTT+AGG 0.381386 4.2:-51958146 None:intergenic
GTCACCATTGCTATTTCCTT+CGG 0.382814 4.2:-51959447 None:intergenic
CTTGTTTCATGAGATGAGAT+TGG 0.387183 4.2:-51955715 None:intergenic
TTTCAGATGGCTATTCTTCA+AGG 0.389126 4.2:+51957284 MS.gene061319:intron
AAAAGGAAATTTGATGATGC+TGG 0.391734 4.2:+51957623 MS.gene061319:CDS
GCTGCTGATAATAATAATAA+TGG 0.393320 4.2:+51959413 MS.gene061319:CDS
TCTTTGGCTGCTGCAGGTAA+AGG 0.394516 4.2:-51958921 None:intergenic
TCAATTGTAATAGTTGGATA+TGG 0.399033 4.2:-51959086 None:intergenic
TAACATCCTCATTCTTACTT+AGG 0.406214 4.2:-51957892 None:intergenic
GCAGCAGCAGTGTTGTTGTT+TGG 0.408470 4.2:-51959395 None:intergenic
CTTTAACTTTCTTACCCTTA+CGG 0.409546 4.2:-51957415 None:intergenic
TCAAGGTTGTTAGTATCATC+AGG 0.414586 4.2:-51955598 None:intergenic
CTTTGGCTGCTGCAGGTAAA+GGG 0.420375 4.2:-51958920 None:intergenic
GTGTAATCAATTGTAATAGT+TGG 0.425606 4.2:-51959092 None:intergenic
AACTTACATGCTTTCGGACT+GGG 0.430854 4.2:-51958184 None:intergenic
AAAATGAGAATTAAGAATGA+TGG 0.434196 4.2:+51957590 MS.gene061319:CDS
TGGCTGAGTATCACCAATAA+TGG 0.440113 4.2:-51959375 None:intergenic
GTATGAATATGATCATTGTC+AGG 0.443290 4.2:-51955802 None:intergenic
GCATCAACATCAAGCAATGA+TGG 0.448071 4.2:+51958987 MS.gene061319:CDS
GACCTAATATTGACTATGAA+AGG 0.455076 4.2:-51955670 None:intergenic
ACCTGCAGCAGCCAAAGAAA+TGG 0.461509 4.2:+51958926 MS.gene061319:CDS
AGCTTTAGTTCCAATAAAGA+TGG 0.463299 4.2:-51955832 None:intergenic
CCAAGGAAATTTATGGACCT+TGG 0.465600 4.2:+51957503 MS.gene061319:CDS
ATCGGCTACTGATAAGGTTA+AGG 0.465620 4.2:+51957526 MS.gene061319:CDS
CCTTGGATCGGCTACTGATA+AGG 0.469293 4.2:+51957520 MS.gene061319:CDS
CCAAGGTCCATAAATTTCCT+TGG 0.469548 4.2:-51957503 None:intergenic
CCTAGAAGTCAAGATCAGTT+AGG 0.482042 4.2:+51957827 MS.gene061319:CDS
ACATGTGGCATCAACAATGA+TGG 0.482281 4.2:+51959509 MS.gene061319:CDS
GAAATTTGATGATGCTGGAT+TGG 0.483257 4.2:+51957628 MS.gene061319:CDS
ATTTGAGGGATCAGAGAAGC+TGG 0.491231 4.2:-51959191 None:intergenic
GTTGGTGCCAAGGCAATGTA+AGG 0.498458 4.2:+51957736 MS.gene061319:CDS
AGTATTGATAGTAATAGGAT+TGG 0.501708 4.2:-51956031 None:intergenic
GCTTTCGGACTGGGCAACCA+TGG 0.504496 4.2:-51958175 None:intergenic
ATGATGGAGAATTAATGAAA+AGG 0.504955 4.2:+51957606 MS.gene061319:CDS
AAACTTACATGCTTTCGGAC+TGG 0.512195 4.2:-51958185 None:intergenic
GAGAGTGATGCAAGACCGTA+AGG 0.513027 4.2:+51957400 MS.gene061319:CDS
GGTATTGCCAATATTTGTGA+AGG 0.514432 4.2:-51959272 None:intergenic
TAGTAGTGATCAATCTATGA+TGG 0.520634 4.2:+51956053 MS.gene061319:CDS
TCATGAAACAAGAATAGAGT+TGG 0.523713 4.2:+51955726 MS.gene061319:CDS
ACGAGATCCTTACATTGCCT+TGG 0.527338 4.2:-51957743 None:intergenic
CGATAATAAGCTTTAGGACA+CGG 0.531100 4.2:-51958140 None:intergenic
GAAATTTATGGACCTTGGAT+CGG 0.533334 4.2:+51957508 MS.gene061319:CDS
TGGGGCTAAGTTTCAACTCA+AGG 0.537331 4.2:-51955615 None:intergenic
GTATTGATAGTAATAGGATT+GGG 0.539424 4.2:-51956030 None:intergenic
GGGAATTTGCAAAACTCTGA+GGG 0.541731 4.2:-51959169 None:intergenic
TTATTGAGTTAGATCACTGA+TGG 0.542017 4.2:+51958074 MS.gene061319:intron
GTTGTAATCACAACTTATGA+AGG 0.543545 4.2:+51958885 MS.gene061319:CDS
CTTTCGGACTGGGCAACCAT+GGG 0.546480 4.2:-51958174 None:intergenic
ACTTCAGAATGGTGGAGAGT+TGG 0.548067 4.2:+51957718 MS.gene061319:CDS
ACCTAATATTGACTATGAAA+GGG 0.556527 4.2:-51955669 None:intergenic
AAAGGAAAAGAAGTGTTCAC+TGG 0.557236 4.2:+51957674 MS.gene061319:CDS
TATCGCTGCAGCACGACCCA+TGG 0.564450 4.2:+51958158 MS.gene061319:CDS
ATAGCAGCTGACAAAGCTGC+AGG 0.574982 4.2:-51959347 None:intergenic
GAAGAGAACTAAGAGTATGG+AGG 0.575623 4.2:+51956104 MS.gene061319:CDS
ATGGTGGAGTACTGAGGCCA+AGG 0.575809 4.2:+51957486 MS.gene061319:CDS
GATAATAAGCTTTAGGACAC+GGG 0.575823 4.2:-51958139 None:intergenic
GATCAATCTATGATGGATGA+TGG 0.577380 4.2:+51956060 MS.gene061319:CDS
AGATCACTGATGGATGTCAA+TGG 0.579642 4.2:+51958084 MS.gene061319:intron
AGCAACAAAATTATCACATG+TGG 0.580366 4.2:+51959494 MS.gene061319:CDS
GTATTGCCAATATTTGTGAA+GGG 0.582929 4.2:-51959271 None:intergenic
TTTGAGGGATCAGAGAAGCT+GGG 0.583186 4.2:-51959190 None:intergenic
CATGTGGCATCAACAATGAT+GGG 0.583795 4.2:+51959510 MS.gene061319:CDS
GATAGTCCTAAGTAAGAATG+AGG 0.591251 4.2:+51957886 MS.gene061319:CDS
ATGATCATTGTCAGGGACAG+AGG 0.594495 4.2:-51955794 None:intergenic
TATGAATATGATCATTGTCA+GGG 0.594834 4.2:-51955801 None:intergenic
GGGGAATTTGCAAAACTCTG+AGG 0.594846 4.2:-51959170 None:intergenic
ATGGTGGAGAGTTGGTGCCA+AGG 0.595504 4.2:+51957726 MS.gene061319:CDS
CATCAACATCAAGCAATGAT+GGG 0.596489 4.2:+51958988 MS.gene061319:CDS
TGGCATCAACAATGATGGGA+AGG 0.597443 4.2:+51959514 MS.gene061319:CDS
ATTAACTGGAACTAAAGCCA+TGG 0.600498 4.2:-51955563 None:intergenic
AATATTTGGACTAGCAGCAA+TGG 0.612014 4.2:-51959324 None:intergenic
CCTTATCAGTAGCCGATCCA+AGG 0.612290 4.2:-51957520 None:intergenic
GATGTCAATGGAGAAAGTAT+GGG 0.615340 4.2:+51958096 MS.gene061319:CDS
TTAAAACTTAACAGGTACAA+AGG 0.618245 4.2:+51958844 MS.gene061319:intron
TGTTAATGTTGATAATGTTG+AGG 0.619880 4.2:+51957910 MS.gene061319:CDS
TGGCTGCTGCAGGTAAAGGG+TGG 0.624702 4.2:-51958917 None:intergenic
CAAGGTTGTTAGTATCATCA+GGG 0.625070 4.2:-51955597 None:intergenic
AATGGCAACAATGTCACCGA+AGG 0.626879 4.2:+51959431 MS.gene061319:CDS
TACAAATGCTGAGACTGTGA+TGG 0.629505 4.2:+51957772 MS.gene061319:CDS
TTGAGGGATCAGAGAAGCTG+GGG 0.638371 4.2:-51959189 None:intergenic
GTCACCGAAGGAAATAGCAA+TGG 0.641476 4.2:+51959443 MS.gene061319:CDS
TTCATGAGATGAGATTGGAG+AGG 0.650096 4.2:-51955710 None:intergenic
AGGCAGAGACAACTTCATGA+TGG 0.653739 4.2:-51955774 None:intergenic
GAATAGATTACAATTACATG+CGG 0.660089 4.2:+51957366 MS.gene061319:CDS
AGCTAGATCAGAAGAAACCG+TGG 0.677753 4.2:+51957964 MS.gene061319:CDS
AATCTCCAAATACTCCACAA+AGG 0.678231 4.2:+51959117 MS.gene061319:CDS
CTGAGAATGGTGGAGTACTG+AGG 0.679110 4.2:+51957480 MS.gene061319:CDS
GTTGCAATACTTGAAGAACA+AGG 0.694121 4.2:-51959047 None:intergenic
AGAGTGATGCAAGACCGTAA+GGG 0.707223 4.2:+51957401 MS.gene061319:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAAATAATAATAAT+GGG - chr4.2:51955884-51955903 None:intergenic 0.0%
!! AAATATAATCACATAAAAAA+CGG - chr4.2:51957248-51957267 None:intergenic 10.0%
!! ATATATATATATATGATTCT+TGG + chr4.2:51956398-51956417 MS.gene061319:intron 10.0%
!! TATAACATAATTATTATTTG+TGG - chr4.2:51956541-51956560 None:intergenic 10.0%
!!! TTATTTTCTCAAATTATTAT+TGG + chr4.2:51956952-51956971 MS.gene061319:intron 10.0%
!! AAAAAATTTAAAAGAAAGTG+AGG - chr4.2:51956323-51956342 None:intergenic 15.0%
!! TAATACAATTAAAACTTAAC+AGG + chr4.2:51958836-51958855 MS.gene061319:intron 15.0%
!! TAATTAAATTACTTATGAAG+TGG + chr4.2:51958792-51958811 MS.gene061319:intron 15.0%
!! TGTATTATACTTATAAATGT+AGG - chr4.2:51956465-51956484 None:intergenic 15.0%
!! TTTAGTAAATACATGTAATT+TGG - chr4.2:51958224-51958243 None:intergenic 15.0%
!!! ACTATGTTATATTTTTTGAA+AGG + chr4.2:51956345-51956364 MS.gene061319:intron 15.0%
!!! TTCATTTTTTAAAAGATACT+AGG + chr4.2:51956782-51956801 MS.gene061319:intron 15.0%
!!! TTTTGACTCAATAAAATTAA+AGG - chr4.2:51956187-51956206 None:intergenic 15.0%
!! AAAATGAGAATTAAGAATGA+TGG + chr4.2:51957590-51957609 MS.gene061319:CDS 20.0%
!! AAATAATGATTTAAAGACCA+AGG - chr4.2:51958421-51958440 None:intergenic 20.0%
!! ATATATATATGATTCTTGGT+TGG + chr4.2:51956402-51956421 MS.gene061319:intron 20.0%
!! TAATTTCATTAAATTCTGCT+AGG - chr4.2:51956144-51956163 None:intergenic 20.0%
!!! AATAATGGGATATTGAATTA+TGG - chr4.2:51955870-51955889 None:intergenic 20.0%
!!! AATTTTATTGAGTCAAAACT+GGG + chr4.2:51956189-51956208 MS.gene061319:intron 20.0%
!!! AATTTTGATTGGTTTTGATT+AGG - chr4.2:51959218-51959237 None:intergenic 20.0%
!!! TAATTTTATTGAGTCAAAAC+TGG + chr4.2:51956188-51956207 MS.gene061319:intron 20.0%
!!! TAATTTTTTTCACAAAGAAG+AGG - chr4.2:51957057-51957076 None:intergenic 20.0%
! AAAATCTAACAGTTTATGTC+AGG - chr4.2:51956926-51956945 None:intergenic 25.0%
! AAAGGAAAAAAAGTCTTTGA+TGG + chr4.2:51957434-51957453 MS.gene061319:CDS 25.0%
! ATTTAAATAGCGTACTATGT+CGG - chr4.2:51956729-51956748 None:intergenic 25.0%
! CAATAAACTCAAATCCAAAA+TGG + chr4.2:51958752-51958771 MS.gene061319:intron 25.0%
! CACTGACATACATATAAAAT+CGG - chr4.2:51957007-51957026 None:intergenic 25.0%
! CCAACAAAAACAAAATACAT+TGG - chr4.2:51957148-51957167 None:intergenic 25.0%
! GAATAGATTACAATTACATG+CGG + chr4.2:51957366-51957385 MS.gene061319:CDS 25.0%
! GATAAGTTTAAGGAAGTTAA+AGG + chr4.2:51957656-51957675 MS.gene061319:CDS 25.0%
! GCTGCTGATAATAATAATAA+TGG + chr4.2:51959413-51959432 MS.gene061319:CDS 25.0%
! GTCATATTCATTAATTAACG+TGG - chr4.2:51956633-51956652 None:intergenic 25.0%
! GTGTAATCAATTGTAATAGT+TGG - chr4.2:51959095-51959114 None:intergenic 25.0%
! TAAATACATAGATTGCTGAA+TGG - chr4.2:51958614-51958633 None:intergenic 25.0%
! TAGAACACTATTAACATGAT+GGG + chr4.2:51957120-51957139 MS.gene061319:intron 25.0%
! TATGAATATGATCATTGTCA+GGG - chr4.2:51955804-51955823 None:intergenic 25.0%
! TCAAAATATGCAGTTATGTT+TGG + chr4.2:51958662-51958681 MS.gene061319:intron 25.0%
! TCAATTGTAATAGTTGGATA+TGG - chr4.2:51959089-51959108 None:intergenic 25.0%
! TTAAAACTTAACAGGTACAA+AGG + chr4.2:51958844-51958863 MS.gene061319:intron 25.0%
! TTAGAACACTATTAACATGA+TGG + chr4.2:51957119-51957138 MS.gene061319:intron 25.0%
!! AATCACATAAAAAACGGATT+TGG - chr4.2:51957242-51957261 None:intergenic 25.0%
!! ACCTAATATTGACTATGAAA+GGG - chr4.2:51955672-51955691 None:intergenic 25.0%
!! ACTAAAAATGTTTCTGTATG+TGG - chr4.2:51958476-51958495 None:intergenic 25.0%
!! ATGATGGAGAATTAATGAAA+AGG + chr4.2:51957606-51957625 MS.gene061319:CDS 25.0%
!! CAAAACCAATCAAAATTTTC+TGG + chr4.2:51959221-51959240 MS.gene061319:CDS 25.0%
!! CATGTTAATAGTGTTCTAAA+AGG - chr4.2:51957118-51957137 None:intergenic 25.0%
!! CCTCAATACTCAAATTTTTA+GGG + chr4.2:51956878-51956897 MS.gene061319:intron 25.0%
!! GTATTGATAGTAATAGGATT+GGG - chr4.2:51956033-51956052 None:intergenic 25.0%
!! TATGTTTCTATGCTAGAAAA+TGG + chr4.2:51958575-51958594 MS.gene061319:intron 25.0%
!! TGTGATAATTTTGTTGCTAT+TGG - chr4.2:51959492-51959511 None:intergenic 25.0%
!! TTCATTGAACTATTTTCAGA+TGG + chr4.2:51957271-51957290 MS.gene061319:intron 25.0%
!!! ACATGCATATTTTAAAACCT+TGG + chr4.2:51958401-51958420 MS.gene061319:intron 25.0%
!!! AGTATTGATAGTAATAGGAT+TGG - chr4.2:51956034-51956053 None:intergenic 25.0%
!!! CCAATGTATTTTGTTTTTGT+TGG + chr4.2:51957145-51957164 MS.gene061319:intron 25.0%
!!! CCTAAAAATTTGAGTATTGA+GGG - chr4.2:51956880-51956899 None:intergenic 25.0%
!!! CTACTAGTATTGATAGTAAT+AGG - chr4.2:51956039-51956058 None:intergenic 25.0%
!!! CTGCATATTTTGATTTACAA+TGG - chr4.2:51958655-51958674 None:intergenic 25.0%
!!! TGTTAATGTTGATAATGTTG+AGG + chr4.2:51957910-51957929 MS.gene061319:CDS 25.0%
AAGAAAAAGAGACTTCAGAA+TGG + chr4.2:51957707-51957726 MS.gene061319:CDS 30.0%
ACGTTCGAATAAAACATGTT+AGG - chr4.2:51957211-51957230 None:intergenic 30.0%
ACTGTTTGAGCAGTAATATT+TGG - chr4.2:51959296-51959315 None:intergenic 30.0%
AGAACACTATTAACATGATG+GGG + chr4.2:51957121-51957140 MS.gene061319:intron 30.0%
AGAAGAAACTTACATGCTTT+CGG - chr4.2:51958193-51958212 None:intergenic 30.0%
AGCAACAAAATTATCACATG+TGG + chr4.2:51959494-51959513 MS.gene061319:CDS 30.0%
AGTTTATGTCAGGATCATAT+TGG - chr4.2:51956916-51956935 None:intergenic 30.0%
ATAATGCGAAAGCAATCAAT+AGG - chr4.2:51958265-51958284 None:intergenic 30.0%
ATTGGCTACTGATAAGTTTA+AGG + chr4.2:51957646-51957665 MS.gene061319:CDS 30.0%
CGTTCGAATAAAACATGTTA+GGG - chr4.2:51957210-51957229 None:intergenic 30.0%
GATAAGGTTAAGGAAGTTAA+AGG + chr4.2:51957536-51957555 MS.gene061319:CDS 30.0%
GTATGAATATGATCATTGTC+AGG - chr4.2:51955805-51955824 None:intergenic 30.0%
GTATTGCCAATATTTGTGAA+GGG - chr4.2:51959274-51959293 None:intergenic 30.0%
GTTGAAATAAAGAGCAGAAA+TGG - chr4.2:51956519-51956538 None:intergenic 30.0%
GTTGTAATCACAACTTATGA+AGG + chr4.2:51958885-51958904 MS.gene061319:CDS 30.0%
TAACATCCTCATTCTTACTT+AGG - chr4.2:51957895-51957914 None:intergenic 30.0%
TAATGCGAAAGCAATCAATA+GGG - chr4.2:51958264-51958283 None:intergenic 30.0%
TACTGATACACCATCTTTAT+TGG + chr4.2:51955822-51955841 MS.gene061319:CDS 30.0%
TAGTAGTGATCAATCTATGA+TGG + chr4.2:51956053-51956072 MS.gene061319:CDS 30.0%
TCATGAAACAAGAATAGAGT+TGG + chr4.2:51955726-51955745 MS.gene061319:CDS 30.0%
TCCCTTTCATAGTCAATATT+AGG + chr4.2:51955668-51955687 MS.gene061319:CDS 30.0%
TTATTGAGTTAGATCACTGA+TGG + chr4.2:51958074-51958093 MS.gene061319:intron 30.0%
! ACACTTTTACATACTTACCA+CGG - chr4.2:51957984-51958003 None:intergenic 30.0%
! AGCTTTAGTTCCAATAAAGA+TGG - chr4.2:51955835-51955854 None:intergenic 30.0%
! CCCTAAAAATTTGAGTATTG+AGG - chr4.2:51956881-51956900 None:intergenic 30.0%
! CCCTCAATACTCAAATTTTT+AGG + chr4.2:51956877-51956896 MS.gene061319:intron 30.0%
! CTTTAACTTTCTTACCCTTA+CGG - chr4.2:51957418-51957437 None:intergenic 30.0%
! GACCTAATATTGACTATGAA+AGG - chr4.2:51955673-51955692 None:intergenic 30.0%
! TATATGATTCTTGGTTGGTT+TGG + chr4.2:51956407-51956426 MS.gene061319:intron 30.0%
!! AAAAGGAAATTTGATGATGC+TGG + chr4.2:51957623-51957642 MS.gene061319:CDS 30.0%
!! AGAAACATAAGCTTTGAAAG+TGG - chr4.2:51958564-51958583 None:intergenic 30.0%
!! GTAGTCCAGAAAATTTTGAT+TGG - chr4.2:51959229-51959248 None:intergenic 30.0%
!!! AGTTGTTTTTGCCATTTCTT+TGG - chr4.2:51958940-51958959 None:intergenic 30.0%
!!! GGTTTTGATTAGGTATTTGA+GGG - chr4.2:51959208-51959227 None:intergenic 30.0%
!!! TGGTTTTGATTAGGTATTTG+AGG - chr4.2:51959209-51959228 None:intergenic 30.0%
!!! TTCTTTTTTCTCATACAGCT+TGG + chr4.2:51956006-51956025 MS.gene061319:intron 30.0%
AAAAAGAGAACTGAGAATGG+TGG + chr4.2:51957470-51957489 MS.gene061319:CDS 35.0%
AAAAAGAGACTTCAGAATGG+TGG + chr4.2:51957710-51957729 MS.gene061319:CDS 35.0%
AAAATGGTAGCTACTTAGCT+AGG + chr4.2:51958768-51958787 MS.gene061319:intron 35.0%
AATCTCCAAATACTCCACAA+AGG + chr4.2:51959117-51959136 MS.gene061319:CDS 35.0%
ACCCACGTACACAAAAATAT+GGG + chr4.2:51956290-51956309 MS.gene061319:intron 35.0%
AGAGAAGAGAACTAAGAGTA+TGG + chr4.2:51956101-51956120 MS.gene061319:CDS 35.0%
AGAGCTTGAACAAACGAAAA+TGG + chr4.2:51957307-51957326 MS.gene061319:CDS 35.0%
CAAGGTTGTTAGTATCATCA+GGG - chr4.2:51955600-51955619 None:intergenic 35.0%
CATCAACATCAAGCAATGAT+GGG + chr4.2:51958988-51959007 MS.gene061319:CDS 35.0%
CGTAAGGGTAAGAAAGTTAA+AGG + chr4.2:51957416-51957435 MS.gene061319:CDS 35.0%
CTTGTTTCATGAGATGAGAT+TGG - chr4.2:51955718-51955737 None:intergenic 35.0%
GAAATTTATGGACCTTGGAT+CGG + chr4.2:51957508-51957527 MS.gene061319:CDS 35.0%
GAGAAAAAGAGAACTGAGAA+TGG + chr4.2:51957467-51957486 MS.gene061319:CDS 35.0%
GATAGTCCTAAGTAAGAATG+AGG + chr4.2:51957886-51957905 MS.gene061319:CDS 35.0%
GATCTCACTATTACAATGCA+TGG + chr4.2:51957092-51957111 MS.gene061319:intron 35.0%
GCTGCTATTACATCCATTAT+TGG + chr4.2:51959362-51959381 MS.gene061319:CDS 35.0%
GGTATTGCCAATATTTGTGA+AGG - chr4.2:51959275-51959294 None:intergenic 35.0%
GTTCTCTTCTCTTCTGAATT+AGG - chr4.2:51956093-51956112 None:intergenic 35.0%
GTTGCAATACTTGAAGAACA+AGG - chr4.2:51959050-51959069 None:intergenic 35.0%
TATGGGCAAAAACTATCGAA+AGG + chr4.2:51958113-51958132 MS.gene061319:CDS 35.0%
TCAAGGTTGTTAGTATCATC+AGG - chr4.2:51955601-51955620 None:intergenic 35.0%
TCGACACTCTTTAGATTTGA+AGG + chr4.2:51956229-51956248 MS.gene061319:intron 35.0%
TTAAAAGAAAGTGAGGACCT+TGG - chr4.2:51956316-51956335 None:intergenic 35.0%
TTAACGTGGTAGCATATCAT+TGG - chr4.2:51956619-51956638 None:intergenic 35.0%
TTATTGCATTCAGCAAATCC+AGG - chr4.2:51958738-51958757 None:intergenic 35.0%
TTTCAGATGGCTATTCTTCA+AGG + chr4.2:51957284-51957303 MS.gene061319:intron 35.0%
! GATGTCAATGGAGAAAGTAT+GGG + chr4.2:51958096-51958115 MS.gene061319:CDS 35.0%
! GTAGCATCCATCTTTTTGTT+GGG - chr4.2:51955637-51955656 None:intergenic 35.0%
! TAGCATCCATCTTTTTGTTG+GGG - chr4.2:51955636-51955655 None:intergenic 35.0%
! TAGCTAAGTAGCTACCATTT+TGG - chr4.2:51958769-51958788 None:intergenic 35.0%
! TGTAGCATCCATCTTTTTGT+TGG - chr4.2:51955638-51955657 None:intergenic 35.0%
!! AAAGGAAAAGAAGTGTTCAC+TGG + chr4.2:51957674-51957693 MS.gene061319:CDS 35.0%
!! AATATTTGGACTAGCAGCAA+TGG - chr4.2:51959327-51959346 None:intergenic 35.0%
!! AGAGGTTTGATTTTCATTGG+AGG - chr4.2:51955695-51955714 None:intergenic 35.0%
!! ATGTTTTATTCGAACGTGAG+AGG + chr4.2:51957214-51957233 MS.gene061319:intron 35.0%
!! CGATAATAAGCTTTAGGACA+CGG - chr4.2:51958143-51958162 None:intergenic 35.0%
!! GAAATTTGATGATGCTGGAT+TGG + chr4.2:51957628-51957647 MS.gene061319:CDS 35.0%
!! GATAATAAGCTTTAGGACAC+GGG - chr4.2:51958142-51958161 None:intergenic 35.0%
!! GATCAATCTATGATGGATGA+TGG + chr4.2:51956060-51956079 MS.gene061319:CDS 35.0%
!! TCTTTAGATTTGAAGGTGTC+TGG + chr4.2:51956236-51956255 MS.gene061319:intron 35.0%
!! TGGAGAGGTTTGATTTTCAT+TGG - chr4.2:51955698-51955717 None:intergenic 35.0%
AAACTTACATGCTTTCGGAC+TGG - chr4.2:51958188-51958207 None:intergenic 40.0%
AACTTACATGCTTTCGGACT+GGG - chr4.2:51958187-51958206 None:intergenic 40.0%
ACATGTGGCATCAACAATGA+TGG + chr4.2:51959509-51959528 MS.gene061319:CDS 40.0%
ACTGAGGCCAAGGAAATTTA+TGG + chr4.2:51957496-51957515 MS.gene061319:CDS 40.0%
ACTTAGCCCCAACAAAAAGA+TGG + chr4.2:51955627-51955646 MS.gene061319:CDS 40.0%
AGCTGACCCTTCACAAATAT+TGG + chr4.2:51959265-51959284 MS.gene061319:CDS 40.0%
ATCGGCTACTGATAAGGTTA+AGG + chr4.2:51957526-51957545 MS.gene061319:CDS 40.0%
CATGTGGCATCAACAATGAT+GGG + chr4.2:51959510-51959529 MS.gene061319:CDS 40.0%
CATGTTTGTGACTGTGATCT+AGG - chr4.2:51958386-51958405 None:intergenic 40.0%
CCAAGGAAATTTATGGACCT+TGG + chr4.2:51957503-51957522 MS.gene061319:CDS 40.0%
CCAAGGTCCATAAATTTCCT+TGG - chr4.2:51957506-51957525 None:intergenic 40.0%
CCTAACTGATCTTGACTTCT+AGG - chr4.2:51957830-51957849 None:intergenic 40.0%
CCTAGAAGTCAAGATCAGTT+AGG + chr4.2:51957827-51957846 MS.gene061319:CDS 40.0%
CTGCAGCGATAATAAGCTTT+AGG - chr4.2:51958149-51958168 None:intergenic 40.0%
GACAAAGCTGCAGGAATATT+TGG - chr4.2:51959341-51959360 None:intergenic 40.0%
GACCCACGTACACAAAAATA+TGG + chr4.2:51956289-51956308 MS.gene061319:intron 40.0%
GATTTGATATGCCTTGCACA+TGG + chr4.2:51958504-51958523 MS.gene061319:intron 40.0%
GCATCAACATCAAGCAATGA+TGG + chr4.2:51958987-51959006 MS.gene061319:CDS 40.0%
GGATCATATTGGTGCAATGA+CGG - chr4.2:51956905-51956924 None:intergenic 40.0%
GGATGTCAATGGAGAAAGTA+TGG + chr4.2:51958095-51958114 MS.gene061319:CDS 40.0%
GGGAAAGAGTTGTCATTAAC+TGG - chr4.2:51955580-51955599 None:intergenic 40.0%
GGGAATTTGCAAAACTCTGA+GGG - chr4.2:51959172-51959191 None:intergenic 40.0%
GTACACAAAAATATGGGCCA+AGG + chr4.2:51956296-51956315 MS.gene061319:intron 40.0%
TACAAATGCTGAGACTGTGA+TGG + chr4.2:51957772-51957791 MS.gene061319:CDS 40.0%
TGGCTGAGTATCACCAATAA+TGG - chr4.2:51959378-51959397 None:intergenic 40.0%
TTACAATGGCCAGATGTGTT+GGG - chr4.2:51958641-51958660 None:intergenic 40.0%
TTCATGAGATGAGATTGGAG+AGG - chr4.2:51955713-51955732 None:intergenic 40.0%
TTGTTATCCAACCATGTGCA+AGG - chr4.2:51958518-51958537 None:intergenic 40.0%
TTTACAATGGCCAGATGTGT+TGG - chr4.2:51958642-51958661 None:intergenic 40.0%
! AGATCACTGATGGATGTCAA+TGG + chr4.2:51958084-51958103 MS.gene061319:intron 40.0%
! GAAGAGAACTAAGAGTATGG+AGG + chr4.2:51956104-51956123 MS.gene061319:CDS 40.0%
! TCTAGGTTTTCTCATTGACG+AGG - chr4.2:51957813-51957832 None:intergenic 40.0%
!! GCCCATATTTTTGTGTACGT+GGG - chr4.2:51956294-51956313 None:intergenic 40.0%
!! GTCACCATTGCTATTTCCTT+CGG - chr4.2:51959450-51959469 None:intergenic 40.0%
AACTGATCTGCGTTCCTTTG+TGG - chr4.2:51959134-51959153 None:intergenic 45.0%
AATGGCAACAATGTCACCGA+AGG + chr4.2:51959431-51959450 MS.gene061319:CDS 45.0%
ACGAGATCCTTACATTGCCT+TGG - chr4.2:51957746-51957765 None:intergenic 45.0%
AGAGTGATGCAAGACCGTAA+GGG + chr4.2:51957401-51957420 MS.gene061319:CDS 45.0%
AGCTAGATCAGAAGAAACCG+TGG + chr4.2:51957964-51957983 MS.gene061319:CDS 45.0%
AGGCAGAGACAACTTCATGA+TGG - chr4.2:51955777-51955796 None:intergenic 45.0%
ATGATCATTGTCAGGGACAG+AGG - chr4.2:51955797-51955816 None:intergenic 45.0%
ATGTGTGACACCCATACAGA+CGG + chr4.2:51956267-51956286 MS.gene061319:intron 45.0%
ATTTACGCACCCAACACATC+TGG + chr4.2:51958629-51958648 MS.gene061319:intron 45.0%
ATTTGAGGGATCAGAGAAGC+TGG - chr4.2:51959194-51959213 None:intergenic 45.0%
GCACGTAATAAGCACAGTGA+TGG - chr4.2:51958712-51958731 None:intergenic 45.0%
GCAGAAATGGATGACTCTCA+TGG - chr4.2:51956506-51956525 None:intergenic 45.0%
GGGGAATTTGCAAAACTCTG+AGG - chr4.2:51959173-51959192 None:intergenic 45.0%
GTCACCGAAGGAAATAGCAA+TGG + chr4.2:51959443-51959462 MS.gene061319:CDS 45.0%
TGATATGCCTTGCACATGGT+TGG + chr4.2:51958508-51958527 MS.gene061319:intron 45.0%
TGGGGCTAAGTTTCAACTCA+AGG - chr4.2:51955618-51955637 None:intergenic 45.0%
TTTGAGGGATCAGAGAAGCT+GGG - chr4.2:51959193-51959212 None:intergenic 45.0%
! ACTTCAGAATGGTGGAGAGT+TGG + chr4.2:51957718-51957737 MS.gene061319:CDS 45.0%
! GCGTTCCTTTGTGGAGTATT+TGG - chr4.2:51959125-51959144 None:intergenic 45.0%
! GTATGGGTGTCACACATTGA+CGG - chr4.2:51956264-51956283 None:intergenic 45.0%
! TATGGGTGTCACACATTGAC+GGG - chr4.2:51956263-51956282 None:intergenic 45.0%
! TGGCATCAACAATGATGGGA+AGG + chr4.2:51959514-51959533 MS.gene061319:CDS 45.0%
!! GGCCCATATTTTTGTGTACG+TGG - chr4.2:51956295-51956314 None:intergenic 45.0%
!! GTAAAAAAAAATAATAATAA+TGG - chr4.2:51955885-51955904 None:intergenic 5.0%
!!! ATTAATTTTTTTTAGTAAAA+AGG - chr4.2:51958052-51958071 None:intergenic 5.0%
!!! TTTTATATTTTTCTTTTATT+TGG + chr4.2:51956758-51956777 MS.gene061319:intron 5.0%
ACCTGCAGCAGCCAAAGAAA+TGG + chr4.2:51958926-51958945 MS.gene061319:CDS 50.0%
ATAGCAGCTGACAAAGCTGC+AGG - chr4.2:51959350-51959369 None:intergenic 50.0%
CCTTATCAGTAGCCGATCCA+AGG - chr4.2:51957523-51957542 None:intergenic 50.0%
CCTTGGATCGGCTACTGATA+AGG + chr4.2:51957520-51957539 MS.gene061319:CDS 50.0%
CTTTGGCTGCTGCAGGTAAA+GGG - chr4.2:51958923-51958942 None:intergenic 50.0%
GAGAGTGATGCAAGACCGTA+AGG + chr4.2:51957400-51957419 MS.gene061319:CDS 50.0%
GTGCTTATTACGTGCATGCC+TGG + chr4.2:51958717-51958736 MS.gene061319:intron 50.0%
TCTTTGGCTGCTGCAGGTAA+AGG - chr4.2:51958924-51958943 None:intergenic 50.0%
TTGAGGGATCAGAGAAGCTG+GGG - chr4.2:51959192-51959211 None:intergenic 50.0%
! GCAGCAGCAGTGTTGTTGTT+TGG - chr4.2:51959398-51959417 None:intergenic 50.0%
!! CTGAGAATGGTGGAGTACTG+AGG + chr4.2:51957480-51957499 MS.gene061319:CDS 50.0%
!! GTTGGTGCCAAGGCAATGTA+AGG + chr4.2:51957736-51957755 MS.gene061319:CDS 50.0%
GCCATTTCTTTGGCTGCTGC+AGG - chr4.2:51958930-51958949 None:intergenic 55.0%
GTACGTGGGTCCGTCTGTAT+GGG - chr4.2:51956280-51956299 None:intergenic 55.0%
TGTACGTGGGTCCGTCTGTA+TGG - chr4.2:51956281-51956300 None:intergenic 55.0%
! CTTTCGGACTGGGCAACCAT+GGG - chr4.2:51958177-51958196 None:intergenic 55.0%
!! ATGGTGGAGAGTTGGTGCCA+AGG + chr4.2:51957726-51957745 MS.gene061319:CDS 55.0%
!! ATGGTGGAGTACTGAGGCCA+AGG + chr4.2:51957486-51957505 MS.gene061319:CDS 55.0%
TATCGCTGCAGCACGACCCA+TGG + chr4.2:51958158-51958177 MS.gene061319:CDS 60.0%
TGGCTGCTGCAGGTAAAGGG+TGG - chr4.2:51958920-51958939 None:intergenic 60.0%
! GCTTTCGGACTGGGCAACCA+TGG - chr4.2:51958178-51958197 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 51955565 51959541 51955565 ID=MS.gene061319
chr4.2 mRNA 51955565 51959541 51955565 ID=MS.gene061319.t1;Parent=MS.gene061319
chr4.2 exon 51955565 51955858 51955565 ID=MS.gene061319.t1.exon1;Parent=MS.gene061319.t1
chr4.2 CDS 51955565 51955858 51955565 ID=cds.MS.gene061319.t1;Parent=MS.gene061319.t1
chr4.2 exon 51956024 51956125 51956024 ID=MS.gene061319.t1.exon2;Parent=MS.gene061319.t1
chr4.2 CDS 51956024 51956125 51956024 ID=cds.MS.gene061319.t1;Parent=MS.gene061319.t1
chr4.2 exon 51957290 51957985 51957290 ID=MS.gene061319.t1.exon3;Parent=MS.gene061319.t1
chr4.2 CDS 51957290 51957985 51957290 ID=cds.MS.gene061319.t1;Parent=MS.gene061319.t1
chr4.2 exon 51958086 51958199 51958086 ID=MS.gene061319.t1.exon4;Parent=MS.gene061319.t1
chr4.2 CDS 51958086 51958199 51958086 ID=cds.MS.gene061319.t1;Parent=MS.gene061319.t1
chr4.2 exon 51958858 51959541 51958858 ID=MS.gene061319.t1.exon5;Parent=MS.gene061319.t1
chr4.2 CDS 51958858 51959541 51958858 ID=cds.MS.gene061319.t1;Parent=MS.gene061319.t1
Gene Sequence

>MS.gene061319

ATGGCTTTAGTTCCAGTTAATGACAACTCTTTCCCTGATGATACTAACAACCTTGAGTTGAAACTTAGCCCCAACAAAAAGATGGATGCTACACATAGTACTATCCCTTTCATAGTCAATATTAGGTCCTCCAATGAAAATCAAACCTCTCCAATCTCATCTCATGAAACAAGAATAGAGTTGGATTTCTTTGATGACAACAACAATGACCATCATGAAGTTGTCTCTGCCTCTGTCCCTGACAATGATCATATTCATACTGATACACCATCTTTATTGGAACTAAAGCTAAGCGTGAGTGACCATAATTCAATATCCCATTATTATTATTTTTTTTTACGTATTATATTATATATATTGCTTTTAAATTTCATAGCTTTTGAATCTGTAATCGCAAAAAAACATTTATTTTTTATGATTATCTTTTTAATTTGATGAATATTCTTTTTTCTCATACAGCTTGGTCCCAATCCTATTACTATCAATACTAGTAGTGATCAATCTATGATGGATGATGGCATGTCACCTAATTCAGAAGAGAAGAGAACTAAGAGTATGGAGGTAATGTTATTAACACCTAGCAGAATTTAATGAAATTATATATGATGATCATTTTTTTCCTTTAATTTTATTGAGTCAAAACTGGGTGATTTTATATATTGCATCGACACTCTTTAGATTTGAAGGTGTCTGGACCCGTCAATGTGTGACACCCATACAGACGGACCCACGTACACAAAAATATGGGCCAAGGTCCTCACTTTCTTTTAAATTTTTTCTACTATGTTATATTTTTTGAAAGGAAATATATATATATATATATATATATATATATATATATATATATGATTCTTGGTTGGTTTGGCTTGTTAGTTTTTGTCTCTTACTCTTACATGACCTACATTTATAAGTATAATACAATATAATGTTATATGCAACCATGAGAGTCATCCATTTCTGCTCTTTATTTCAACCACAAATAATAATTATGTTATATTGAGTTAATTTAACTAGTATATTTTGTCAACGTACAATTATTTTATACATGCATCCAATGATATGCTACCACGTTAATTAATGAATATGACAACTCATGTGTTTTCATATTTATTAATTGATGTAGCAGCACATCATTGAATGAATGTGTAAAATAATTGTACACCGACATAGTACGCTATTTAAATTCTTTCATATTTTATATTTTTCTTTTATTTGGTTTCATTTTTTAAAAGATACTAGGTTTAATATTTTAAATGTAAATTGAATTTATTTATTCATTAATTTATTTATTTATTCATTATTTTAAATGCTGCCCTCAATACTCAAATTTTTAGGGTCCGTCATTGCACCAATATGATCCTGACATAAACTGTTAGATTTTGTTGTTTTATTTTCTCAAATTATTATTGGTTGTTGACGTGTCAGTTTTAATGTTTTGTCCGATTTTATATGTATGTCAGTGTTTATAGAATATTTTTGTGATTTGAATCCTCTTCTTTGTGAAAAAAATTACTGAGTTATGTTAATGATCTCACTATTACAATGCATGGCCTTTTAGAACACTATTAACATGATGGGGTCCAATGTATTTTGTTTTTGTTGGATAATCGAATGCAATTTAATAGTGAAATGATTATTATAACCCTAACATGTTTTATTCGAACGTGAGAGGAGCCAAATCCGTTTTTTATGTGATTATATTTGAGTTCATTGAACTATTTTCAGATGGCTATTCTTCAAGGAGAGCTTGAACAAACGAAAATGGAGAACTGTAGACTAAAATTGATGTATGATCTGCTTAGAATAGATTACAATTACATGCGGAAGCGTTTCGAGAGAGTGATGCAAGACCGTAAGGGTAAGAAAGTTAAAGGAAAAAAAGTCTTTGATGGAAAATTTAAAGAGAAAAAGAGAACTGAGAATGGTGGAGTACTGAGGCCAAGGAAATTTATGGACCTTGGATCGGCTACTGATAAGGTTAAGGAAGTTAAAGGACAAAAAGTTTCTGATCAAAAATTTGTAGAGAAAATGAGAATTAAGAATGATGGAGAATTAATGAAAAGGAAATTTGATGATGCTGGATTGGCTACTGATAAGTTTAAGGAAGTTAAAGGAAAAGAAGTGTTCACTGGAAAATGTGAAAAGAAAAAGAGACTTCAGAATGGTGGAGAGTTGGTGCCAAGGCAATGTAAGGATCTCGTGTTGATTACAAATGCTGAGACTGTGATGGATCACAAAGCTTCGTCCTCGTCAATGAGAAAACCTAGAAGTCAAGATCAGTTAGGATCAACGATGAAAAGCATCGATGTTGCTTCGAATGAGATAGTCCTAAGTAAGAATGAGGATGTTAATGTTGATAATGTTGAGGATTCATTGACAAAAGCAAGAGTTTGTATTAGAGCTAGATCAGAAGAAACCGTGGTAAGTATGTAAAAGTGTATATTAACTTTCAACTTTGCAAGTTTTTATATCATAATTTATTGTCCTTTTTACTAAAAAAAATTAATTTTTATTGAGTTAGATCACTGATGGATGTCAATGGAGAAAGTATGGGCAAAAACTATCGAAAGGAAACCCGTGTCCTAAAGCTTATTATCGCTGCAGCACGACCCATGGTTGCCCAGTCCGAAAGCATGTAAGTTTCTTCTGTAAATATCCAAATTACATGTATTTACTAAAGTTTTAAATTTATGTCGCCCTATTGATTGCTTTCGCATTATTATATTTAATAGTATTATAGAGAATTATAGTAAAAAATATGATTAATGCAACATCAATAACATTAAATTCGCAACGACATTAAACTCTTTGATTGTTGCCTAGATCACAGTCACAAACATGCATATTTTAAAACCTTGGTCTTTAAATCATTATTTTATGTATGTTTTCAATTAATTAAAAATTAACTCCACATACAGAAACATTTTTAGTAATTGATTGATTTGATATGCCTTGCACATGGTTGGATAACAATATAATTTAATTGAAATTTGTTTCCACTTTCAAAGCTTATGTTTCTATGCTAGAAAATGGATTTGTATTTGCTCCATTCAGCAATCTATGTATTTACGCACCCAACACATCTGGCCATTGTAAATCAAAATATGCAGTTATGTTTGGATCATTCAATCTTCGATACAACGACCATCACTGTGCTTATTACGTGCATGCCTGGATTTGCTGAATGCAATAAACTCAAATCCAAAATGGTAGCTACTTAGCTAGGTTAATTAAATTACTTATGAAGTGGTCTGTAATGTAATGACATATTTAATACAATTAAAACTTAACAGGTACAAAGGTGTGCTCTTGACAAAACAGTTGTAATCACAACTTATGAAGGAAATCACAACCACCCTTTACCTGCAGCAGCCAAAGAAATGGCAAAAACAACTTCAACAGCTGCAAAGATGCTTCTTTCAGCATCAACATCAAGCAATGATGGGCAATTAAATGCAAATTTACTCACAAGAACACTTCTTCCTTGTTCTTCAAGTATTGCAACTATATCAGCTTCTGCTCCATATCCAACTATTACAATTGATTACACTCAATCTCCAAATACTCCACAAAGGAACGCAGATCAGTTTCAAACTTCTTTAATCCCTCAGAGTTTTGCAAATTCCCCAGCTTCTCTGATCCCTCAAATACCTAATCAAAACCAATCAAAATTTTCTGGACTACATATGTCGAAAGATGCAGCTGACCCTTCACAAATATTGGCAATACCAAATATTACTGCTCAAACAGTTAATGCTGCCATTGCTGCTAGTCCAAATATTCCTGCAGCTTTGTCAGCTGCTATTACATCCATTATTGGTGATACTCAGCCAAACAACAACACTGCTGCTGCTGATAATAATAATAATGGCAACAATGTCACCGAAGGAAATAGCAATGGTGACAACAATAATGTCACAGAAGCCAATAGCAACAAAATTATCACATGTGGCATCAACAATGATGGGAAGGAATAA
Protein sequence

>MS.gene061319.t1

MALVPVNDNSFPDDTNNLELKLSPNKKMDATHSTIPFIVNIRSSNENQTSPISSHETRIELDFFDDNNNDHHEVVSASVPDNDHIHTDTPSLLELKLSLGPNPITINTSSDQSMMDDGMSPNSEEKRTKSMEMAILQGELEQTKMENCRLKLMYDLLRIDYNYMRKRFERVMQDRKGKKVKGKKVFDGKFKEKKRTENGGVLRPRKFMDLGSATDKVKEVKGQKVSDQKFVEKMRIKNDGELMKRKFDDAGLATDKFKEVKGKEVFTGKCEKKKRLQNGGELVPRQCKDLVLITNAETVMDHKASSSSMRKPRSQDQLGSTMKSIDVASNEIVLSKNEDVNVDNVEDSLTKARVCIRARSEETVITDGCQWRKYGQKLSKGNPCPKAYYRCSTTHGCPVRKHVQRCALDKTVVITTYEGNHNHPLPAAAKEMAKTTSTAAKMLLSASTSSNDGQLNANLLTRTLLPCSSSIATISASAPYPTITIDYTQSPNTPQRNADQFQTSLIPQSFANSPASLIPQIPNQNQSKFSGLHMSKDAADPSQILAIPNITAQTVNAAIAASPNIPAALSAAITSIIGDTQPNNNTAAADNNNNGNNVTEGNSNGDNNNVTEANSNKIITCGINNDGKE