AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061320.t1 MTR_4g047580 66.723 592 117 6 1 564 1 540 0.0 723
MS.gene061320.t1 MTR_4g049760 73.665 562 81 5 6 547 8 522 0.0 714
MS.gene061320.t1 MTR_4g049790 61.960 602 125 11 1 564 28 563 0.0 633
MS.gene061320.t1 MTR_2g088000 41.132 530 229 14 57 562 120 590 1.12e-93 299
MS.gene061320.t1 MTR_2g023930 36.029 544 218 17 33 526 67 530 4.75e-70 235
MS.gene061320.t1 MTR_3g053330 32.031 512 223 17 33 525 39 444 2.69e-60 207
MS.gene061320.t1 MTR_4g132430 51.584 221 88 4 311 513 105 324 6.01e-54 187
MS.gene061320.t1 MTR_4g132430 51.584 221 88 4 311 513 239 458 1.53e-53 189
MS.gene061320.t1 MTR_7g110720 44.672 244 99 8 318 549 174 393 8.40e-47 169
MS.gene061320.t1 MTR_7g110720 44.672 244 99 8 318 549 201 420 1.09e-46 169
MS.gene061320.t1 MTR_2g045360 48.750 160 68 3 304 449 182 341 5.82e-37 142
MS.gene061320.t1 MTR_7g009730 27.324 527 235 20 97 542 40 499 1.48e-27 117
MS.gene061320.t1 MTR_4g094412 35.075 268 121 7 318 540 228 487 1.38e-25 110
MS.gene061320.t1 MTR_4g094412 35.075 268 121 7 318 540 219 478 1.83e-25 110
MS.gene061320.t1 MTR_4g107970 56.579 76 24 1 318 384 163 238 7.14e-22 99.8
MS.gene061320.t1 MTR_4g107970 56.579 76 24 1 318 384 240 315 9.16e-22 99.8
MS.gene061320.t1 MTR_4g007060 34.437 151 66 5 310 451 105 231 3.54e-18 85.5
MS.gene061320.t1 MTR_4g007060 34.437 151 66 5 310 451 139 265 4.71e-18 85.9
MS.gene061320.t1 MTR_7g011820 39.655 116 50 3 290 396 377 481 9.64e-17 83.6
MS.gene061320.t1 MTR_2g105060 55.556 54 24 0 332 385 102 155 9.26e-16 77.4
MS.gene061320.t1 MTR_2g105060 55.556 54 24 0 332 385 177 230 1.02e-15 79.0
MS.gene061320.t1 MTR_5g042920 41.237 97 43 3 303 386 286 381 3.80e-15 78.2
MS.gene061320.t1 MTR_3g106060 58.000 50 21 0 332 381 146 195 4.20e-15 76.6
MS.gene061320.t1 MTR_3g056100 37.615 109 55 3 306 402 244 351 4.56e-15 78.2
MS.gene061320.t1 MTR_3g056100 37.615 109 55 3 306 402 388 495 4.76e-15 78.6
MS.gene061320.t1 MTR_3g056100 37.615 109 55 3 306 402 231 338 5.10e-15 77.8
MS.gene061320.t1 MTR_3g056100 37.615 109 55 3 306 402 375 482 5.34e-15 78.6
MS.gene061320.t1 MTR_1g013790 50.943 53 26 0 332 384 109 161 6.35e-15 75.5
MS.gene061320.t1 MTR_7g109600 47.059 68 33 1 332 399 61 125 1.16e-14 74.7
MS.gene061320.t1 MTR_5g074400 39.496 119 56 4 282 386 365 481 1.21e-14 77.4
MS.gene061320.t1 MTR_3g009470 47.500 80 32 2 316 386 478 556 1.52e-14 77.0
MS.gene061320.t1 MTR_7g071120 48.148 81 32 2 315 386 306 385 2.27e-14 76.3
MS.gene061320.t1 MTR_4g013260 46.835 79 32 2 315 384 318 395 2.81e-14 75.9
MS.gene061320.t1 MTR_5g094430 46.250 80 33 2 316 386 235 313 2.86e-14 75.5
MS.gene061320.t1 MTR_5g094430 46.250 80 33 2 316 386 213 291 3.01e-14 75.5
MS.gene061320.t1 MTR_5g094430 46.250 80 33 2 316 386 347 425 5.11e-14 75.1
MS.gene061320.t1 MTR_2g032030 47.619 63 33 0 332 394 275 337 7.20e-14 74.7
MS.gene061320.t1 MTR_4g082580 41.975 81 37 2 315 386 260 339 7.60e-14 73.9
MS.gene061320.t1 MTR_4g094588 45.000 80 42 2 332 411 186 263 8.42e-14 73.2
MS.gene061320.t1 MTR_7g028415 50.000 70 27 2 332 401 101 162 9.14e-14 70.1
MS.gene061320.t1 MTR_7g028710 50.000 70 27 2 332 401 101 162 9.14e-14 70.1
MS.gene061320.t1 MTR_2g032030 47.619 63 33 0 332 394 275 337 1.08e-13 73.6
MS.gene061320.t1 MTR_3g031220 38.614 101 52 2 294 385 326 425 1.15e-13 73.9
MS.gene061320.t1 MTR_2g033820 50.877 57 27 1 332 388 211 266 1.57e-13 72.8
MS.gene061320.t1 MTR_7g080020 40.000 105 51 3 293 386 451 554 2.95e-13 73.2
MS.gene061320.t1 MTR_8g087000 50.909 55 27 0 332 386 85 139 3.06e-13 70.1
MS.gene061320.t1 MTR_8g087000 50.909 55 27 0 332 386 84 138 3.31e-13 70.1
MS.gene061320.t1 MTR_1g013760 43.478 69 39 0 332 400 148 216 4.06e-13 70.5
MS.gene061320.t1 MTR_7g080000 56.364 55 23 1 332 386 440 493 4.77e-13 72.4
MS.gene061320.t1 MTR_5g044695 56.604 53 14 1 311 354 55 107 5.45e-13 65.9
MS.gene061320.t1 MTR_1g086790 44.928 69 33 2 332 400 166 229 6.77e-13 70.5
MS.gene061320.t1 MTR_8g028565 47.619 63 33 0 332 394 163 225 1.07e-12 70.1
MS.gene061320.t1 MTR_8g098945 46.032 63 34 0 332 394 137 199 1.11e-12 68.9
MS.gene061320.t1 MTR_4g094908 52.727 55 26 0 332 386 274 328 1.15e-12 69.7
MS.gene061320.t1 MTR_2g016770 40.506 79 43 1 306 384 21 95 1.15e-12 67.8
MS.gene061320.t1 MTR_1g009613 50.000 60 30 0 332 391 270 329 1.16e-12 69.7
MS.gene061320.t1 MTR_2g016770 40.506 79 43 1 306 384 13 87 1.21e-12 67.8
MS.gene061320.t1 MTR_8g096730 32.381 105 61 2 292 387 29 132 1.41e-12 65.9
MS.gene061320.t1 MTR_3g104750 40.000 90 44 2 302 382 73 161 1.44e-12 66.2
MS.gene061320.t1 MTR_7g079040 47.619 63 33 0 332 394 162 224 1.84e-12 68.9
MS.gene061320.t1 MTR_7g079010 30.409 171 93 5 221 382 45 198 2.55e-12 67.0
MS.gene061320.t1 MTR_2g024350 29.921 127 79 2 332 448 16 142 2.82e-12 67.0
MS.gene061320.t1 MTR_1g099600 39.759 83 48 1 332 414 193 273 3.57e-12 68.2
MS.gene061320.t1 MTR_6g038890 56.604 53 14 1 311 354 166 218 5.10e-12 65.9
MS.gene061320.t1 MTR_7g109800 43.662 71 35 2 332 402 153 218 5.87e-12 67.0
MS.gene061320.t1 MTR_5g091390 42.500 80 36 3 314 388 146 220 8.06e-12 67.0
MS.gene061320.t1 MTR_1g015140 54.902 51 22 1 332 382 110 159 1.20e-11 63.5
MS.gene061320.t1 MTR_2g083870 45.161 62 34 0 332 393 88 149 1.75e-11 65.1
MS.gene061320.t1 MTR_1g070455 52.000 50 24 0 332 381 301 350 2.42e-11 65.9
MS.gene061320.t1 MTR_5g016610 50.000 52 26 0 332 383 207 258 3.65e-11 64.3
MS.gene061320.t1 MTR_4g122530 41.333 75 34 2 319 384 194 267 4.74e-11 65.5
MS.gene061320.t1 MTR_7g100510 52.830 53 24 1 332 384 369 420 6.06e-11 65.1
MS.gene061320.t1 MTR_5g097490 36.957 92 48 2 304 386 223 313 8.02e-11 64.7
MS.gene061320.t1 MTR_2g024380 42.188 64 37 0 332 395 259 322 8.53e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061320.t1 AT4G04450 40.292 479 210 13 70 525 78 503 4.03e-78 256
MS.gene061320.t1 AT4G04450 39.827 462 202 13 87 525 1 409 1.07e-72 239
MS.gene061320.t1 AT1G62300 48.120 266 102 8 310 541 289 552 1.05e-52 189
MS.gene061320.t1 AT4G22070 66.892 148 39 2 312 449 276 423 1.26e-47 174
MS.gene061320.t1 AT4G22070 66.892 148 39 2 312 449 276 423 1.26e-47 174
MS.gene061320.t1 AT4G22070 66.892 148 39 2 312 449 276 423 1.26e-47 174
MS.gene061320.t1 AT4G22070 66.892 148 39 2 312 449 276 423 1.26e-47 174
MS.gene061320.t1 AT4G01720 40.860 279 137 8 267 522 170 443 7.59e-35 137
MS.gene061320.t1 AT1G68150 38.378 185 86 4 277 438 165 344 3.67e-29 119
MS.gene061320.t1 AT5G15130 57.778 90 29 1 318 398 170 259 7.41e-27 114
MS.gene061320.t1 AT1G69810 43.373 166 78 5 304 459 116 275 8.82e-27 111
MS.gene061320.t1 AT5G15130 57.778 90 29 1 318 398 212 301 8.90e-27 114
MS.gene061320.t1 AT1G69810 43.373 166 78 5 304 459 174 333 1.20e-26 112
MS.gene061320.t1 AT1G18860 44.805 154 68 3 316 453 222 374 8.78e-25 108
MS.gene061320.t1 AT1G80840 53.704 54 25 0 332 385 154 207 2.95e-16 80.1
MS.gene061320.t1 AT4G31800 40.244 82 49 0 321 402 108 189 1.17e-15 77.4
MS.gene061320.t1 AT4G31800 40.244 82 49 0 321 402 173 254 1.58e-15 78.2
MS.gene061320.t1 AT2G25000 28.415 183 109 4 226 387 7 188 1.88e-15 77.0
MS.gene061320.t1 AT2G25000 26.500 200 133 4 198 387 14 209 2.11e-15 77.0
MS.gene061320.t1 AT2G25000 39.326 89 45 1 308 387 78 166 5.37e-15 75.1
MS.gene061320.t1 AT2G25000 39.326 89 45 1 308 387 78 166 5.37e-15 75.1
MS.gene061320.t1 AT1G13960 45.570 79 33 2 315 384 391 468 9.94e-15 77.4
MS.gene061320.t1 AT1G13960 45.570 79 33 2 315 384 238 315 1.22e-14 76.3
MS.gene061320.t1 AT2G03340 42.529 87 40 2 309 386 391 476 2.93e-14 75.9
MS.gene061320.t1 AT1G29860 46.479 71 33 2 332 402 144 209 3.14e-14 73.9
MS.gene061320.t1 AT5G56270 60.000 55 21 1 332 386 495 548 4.49e-14 75.9
MS.gene061320.t1 AT2G38470 58.182 55 22 1 332 386 370 423 1.06e-13 74.3
MS.gene061320.t1 AT2G30590 56.140 57 24 1 332 387 321 377 1.31e-13 73.2
MS.gene061320.t1 AT1G64000 46.753 77 31 2 315 382 96 171 1.43e-13 70.1
MS.gene061320.t1 AT5G13080 57.692 52 21 1 332 383 75 125 1.48e-13 68.6
MS.gene061320.t1 AT5G41570 39.394 99 46 3 320 409 85 178 1.98e-13 69.3
MS.gene061320.t1 AT4G18170 38.542 96 48 2 332 417 180 274 2.30e-13 71.6
MS.gene061320.t1 AT5G52830 49.206 63 32 0 332 394 173 235 2.32e-13 72.0
MS.gene061320.t1 AT5G49520 49.123 57 28 1 332 388 229 284 3.00e-13 72.0
MS.gene061320.t1 AT2G23320 55.769 52 23 0 332 383 248 299 3.05e-13 71.2
MS.gene061320.t1 AT3G01080 39.535 86 42 2 308 384 319 403 3.80e-13 72.4
MS.gene061320.t1 AT2G30250 52.632 57 26 1 332 388 336 391 4.12e-13 71.6
MS.gene061320.t1 AT3G01080 39.535 86 42 2 308 384 281 365 4.73e-13 71.6
MS.gene061320.t1 AT3G58710 39.024 82 49 1 307 387 52 133 4.86e-13 70.1
MS.gene061320.t1 AT3G58710 39.024 82 49 1 307 387 53 134 4.88e-13 70.1
MS.gene061320.t1 AT1G30650 48.333 60 31 0 332 391 225 284 5.82e-13 71.6
MS.gene061320.t1 AT2G34830 48.333 60 31 0 332 391 223 282 6.06e-13 71.2
MS.gene061320.t1 AT4G26640 42.500 80 36 2 316 386 188 266 6.30e-13 71.2
MS.gene061320.t1 AT4G26640 42.500 80 36 2 316 386 188 266 6.30e-13 71.2
MS.gene061320.t1 AT4G26640 42.500 80 36 2 316 386 188 266 6.30e-13 71.2
MS.gene061320.t1 AT4G26640 42.500 80 36 2 316 386 188 266 6.30e-13 71.2
MS.gene061320.t1 AT2G34830 48.333 60 31 0 332 391 232 291 6.69e-13 71.2
MS.gene061320.t1 AT5G07100 34.426 122 64 3 266 385 94 201 6.96e-13 68.6
MS.gene061320.t1 AT5G07100 34.426 122 64 3 266 385 94 201 6.96e-13 68.6
MS.gene061320.t1 AT5G07100 34.426 122 64 3 266 385 94 201 6.96e-13 68.6
MS.gene061320.t1 AT5G07100 34.426 122 64 3 266 385 94 201 6.96e-13 68.6
MS.gene061320.t1 AT1G29280 50.943 53 26 0 332 384 82 134 8.27e-13 69.3
MS.gene061320.t1 AT4G01250 47.619 63 33 0 332 394 136 198 8.32e-13 69.7
MS.gene061320.t1 AT4G26640 42.500 80 36 2 316 386 324 402 9.44e-13 71.2
MS.gene061320.t1 AT4G26640 42.500 80 36 2 316 386 292 370 1.02e-12 70.9
MS.gene061320.t1 AT4G26640 42.500 80 36 2 316 386 364 442 1.10e-12 71.2
MS.gene061320.t1 AT5G46350 54.717 53 23 1 332 384 191 242 1.37e-12 69.3
MS.gene061320.t1 AT5G07100 33.600 125 67 3 266 388 187 297 1.82e-12 68.9
MS.gene061320.t1 AT5G26170 44.444 72 30 2 319 381 99 169 3.31e-12 65.5
MS.gene061320.t1 AT5G28650 54.000 50 23 0 332 381 270 319 3.64e-12 68.2
MS.gene061320.t1 AT5G64810 43.617 94 40 3 300 381 74 166 5.70e-12 65.5
MS.gene061320.t1 AT4G24240 53.846 52 24 0 332 383 289 340 6.40e-12 67.8
MS.gene061320.t1 AT3G04670 54.000 50 23 0 332 381 270 319 7.25e-12 67.4
MS.gene061320.t1 AT2G04880 52.830 53 24 1 332 384 291 342 8.17e-12 68.2
MS.gene061320.t1 AT2G04880 52.830 53 24 1 332 384 315 366 1.03e-11 67.8
MS.gene061320.t1 AT3G01970 45.833 72 29 2 319 381 51 121 1.21e-11 63.2
MS.gene061320.t1 AT5G45050 39.744 78 47 0 332 409 1049 1126 1.39e-11 68.2
MS.gene061320.t1 AT5G45050 39.744 78 47 0 332 409 1188 1265 1.41e-11 68.2
MS.gene061320.t1 AT5G45050 39.744 78 47 0 332 409 1160 1237 1.47e-11 68.2
MS.gene061320.t1 AT4G31550 54.000 50 23 0 332 381 254 303 2.21e-11 65.9
MS.gene061320.t1 AT4G31550 54.000 50 23 0 332 381 253 302 2.25e-11 65.9
MS.gene061320.t1 AT4G26440 37.736 106 57 3 332 435 380 478 5.19e-11 65.9
MS.gene061320.t1 AT2G46130 43.056 72 31 2 320 382 17 87 8.90e-11 59.7

Find 106 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAACCGATCCTTACTTAT+AGG 0.138633 4.2:-51981078 None:intergenic
AATAGAGTTTGATTTCTTTA+AGG 0.143956 4.2:+51979051 MS.gene061320:CDS
GACAAAACTGCAGGAAAATT+TGG 0.145250 4.2:-51982561 None:intergenic
GAAGTTTATGATGGAAAATT+TGG 0.238286 4.2:+51980829 MS.gene061320:CDS
GGGTTCCTTTGTGGAGTATT+TGG 0.246074 4.2:-51982375 None:intergenic
TTGACTGCCTGAGCAATATT+TGG 0.249671 4.2:-51982519 None:intergenic
GCAAAAGTCTGACGGATTAA+AGG 0.261046 4.2:-51982414 None:intergenic
GCTACTGATAATAATAATAA+TGG 0.268864 4.2:+51982633 MS.gene061320:CDS
ACTAGTGCCAAGGAAAATTA+TGG 0.320956 4.2:+51980762 MS.gene061320:CDS
AATAAGGTTATGGAAGTTAA+AGG 0.322766 4.2:+51980802 MS.gene061320:CDS
TCCCTTTCCTAGTCAATATT+AGG 0.336762 4.2:+51978981 MS.gene061320:CDS
TCAAGTGTAATGGTTGGAAA+TGG 0.343703 4.2:-51982339 None:intergenic
AAAGGAGTTTGAAACTGATC+TGG 0.348918 4.2:-51982396 None:intergenic
TCTTGGATCGGCTACTAATA+AGG 0.349730 4.2:+51980786 MS.gene061320:CDS
CTGCAACGATAATATGCTTT+AGG 0.352455 4.2:-51981397 None:intergenic
CTTGTTACATTAGATGAGAT+TGG 0.354120 4.2:-51979028 None:intergenic
AACTGATCTGGGTTCCTTTG+TGG 0.354361 4.2:-51982384 None:intergenic
TTGTGTCCCTTCAAAACTAT+TGG 0.363975 4.2:+51982491 MS.gene061320:CDS
AAAAGGAAATTTGTTGATGC+TGG 0.369100 4.2:+51980889 MS.gene061320:CDS
GATGCAAGATTGTAAGGTTA+AGG 0.371759 4.2:+51980672 MS.gene061320:CDS
GCCATTTCTTTGGCTGCTGC+AGG 0.373631 4.2:-51982180 None:intergenic
CCCATCTTTGTTGTTGCTAC+CGG 0.375518 4.2:-51982694 None:intergenic
TAGTGATACATCATCTTTGT+TGG 0.382295 4.2:+51979135 MS.gene061320:CDS
TGCTGGGTTGGCTACTAATA+AGG 0.384058 4.2:+51980906 MS.gene061320:CDS
TCTTTGGCTGCTGCAGGTAA+AGG 0.394516 4.2:-51982174 None:intergenic
AAGGTCAAAGAAGTGTTTAA+TGG 0.396617 4.2:+51980925 MS.gene061320:CDS
CCAAGGAAAATTATGGATCT+TGG 0.397567 4.2:+51980769 MS.gene061320:CDS
TTTCAGATGGCTATTCTTCA+AGG 0.399117 4.2:+51980550 MS.gene061320:intron
TAACATCCTCATTCTTACTT+AGG 0.406214 4.2:-51981143 None:intergenic
CAAGTGTAATGGTTGGAAAT+GGG 0.411437 4.2:-51982338 None:intergenic
TCAGTAGCAGCAGTGTTGTT+TGG 0.417605 4.2:-51982618 None:intergenic
CTTTGGCTGCTGCAGGTAAA+GGG 0.420375 4.2:-51982173 None:intergenic
TAGATGAGATTGGAGAGGTT+TGG 0.425712 4.2:-51979018 None:intergenic
ATCGGCTACTAATAAGGTTA+TGG 0.437213 4.2:+51980792 MS.gene061320:CDS
CTATGAATATGATCCTTGTC+GGG 0.440174 4.2:-51979115 None:intergenic
CTACTGATAATAATAATAAT+GGG 0.441864 4.2:+51982634 MS.gene061320:CDS
GAAAGTGATGCAAGATTGTA+AGG 0.455429 4.2:+51980666 MS.gene061320:CDS
GCTACTCTTACTTCCATGAT+TGG 0.460106 4.2:+51982585 MS.gene061320:CDS
ACCTGCAGCAGCCAAAGAAA+TGG 0.461509 4.2:+51982179 MS.gene061320:CDS
AAAGGACATGAAGTTTATGA+TGG 0.463550 4.2:+51980820 MS.gene061320:CDS
AATGAGAAAACCTATAAGTA+AGG 0.465713 4.2:+51981068 MS.gene061320:CDS
AAGCATCGAGGAGGCTTTGA+AGG 0.465742 4.2:+51981113 MS.gene061320:CDS
AGTTGGTGCAAAGGCAATGT+AGG 0.470135 4.2:+51980986 MS.gene061320:CDS
CAAAAGACAAGAGAACTAAG+AGG 0.470218 4.2:+51979407 MS.gene061320:CDS
ACCGGTAGCAACAACAAAGA+TGG 0.479103 4.2:+51982693 MS.gene061320:CDS
GACCTAATATTGACTAGGAA+AGG 0.479292 4.2:-51978983 None:intergenic
AGTATTGGCAGTAACATGAT+TGG 0.482436 4.2:-51979341 None:intergenic
ACTATGAATATGATCCTTGT+CGG 0.485934 4.2:-51979116 None:intergenic
TGGTTGCTCAGTCCGAAAAC+AGG 0.487721 4.2:+51981429 MS.gene061320:CDS
ACATCAACATCAAGCAATGA+TGG 0.491559 4.2:+51982240 MS.gene061320:CDS
CCTATAAGTAAGGATCGGTT+AGG 0.492432 4.2:+51981078 MS.gene061320:CDS
CTCTGCCTCTGTCCCCGACA+AGG 0.510437 4.2:+51979102 MS.gene061320:CDS
GGCAGTACCAAATATTGCTC+AGG 0.511544 4.2:+51982512 MS.gene061320:CDS
AAGGAGTTTGAAACTGATCT+GGG 0.518182 4.2:-51982395 None:intergenic
TAGTAGTGATCAATCTATGA+TGG 0.520634 4.2:+51979363 MS.gene061320:CDS
CGATAATATGCTTTAGGACA+CGG 0.525997 4.2:-51981391 None:intergenic
ATGGTGGAGAATTAGTGAAA+AGG 0.530673 4.2:+51980872 MS.gene061320:CDS
GAAATTTGTTGATGCTGGGT+TGG 0.532637 4.2:+51980894 MS.gene061320:CDS
GTATTGGCAGTAACATGATT+GGG 0.535076 4.2:-51979340 None:intergenic
TGGCTGAGTATCGCCAATCA+TGG 0.536244 4.2:-51982598 None:intergenic
ACCTAATATTGACTAGGAAA+GGG 0.537487 4.2:-51978982 None:intergenic
GTTGTCATCACAACATATGA+AGG 0.537947 4.2:+51982138 MS.gene061320:CDS
AGGCAGAGACAACTTCTTGA+TGG 0.540615 4.2:-51979087 None:intergenic
CGAGCTTGAACGAAAGAAAA+CGG 0.544425 4.2:+51980573 MS.gene061320:CDS
GAAAATTATGGATCTTGGAT+CGG 0.544647 4.2:+51980774 MS.gene061320:CDS
AGATTGATCACTACTAGTAT+TGG 0.544819 4.2:-51979356 None:intergenic
TTGAGTATAATCAAGTGTAA+TGG 0.545850 4.2:-51982349 None:intergenic
AAAGGAAATTTGTTGATGCT+GGG 0.547546 4.2:+51980890 MS.gene061320:CDS
GTTAGTCCTAAGTAAGAATG+AGG 0.558419 4.2:+51981137 MS.gene061320:CDS
AATTGAGAATGATGGAGAGT+TGG 0.565162 4.2:+51980969 MS.gene061320:CDS
ATGATCCTTGTCGGGGACAG+AGG 0.567680 4.2:-51979107 None:intergenic
AAAATTTGGATTAGCAGCAA+TGG 0.568779 4.2:-51982547 None:intergenic
TTGAGAGATCAGAGAAGCTG+AGG 0.571353 4.2:-51982442 None:intergenic
AGGAATTTGCAAAAGTCTGA+CGG 0.574256 4.2:-51982422 None:intergenic
GATAATATGCTTTAGGACAC+GGG 0.575565 4.2:-51981390 None:intergenic
GTATAATCAAGTGTAATGGT+TGG 0.576480 4.2:-51982345 None:intergenic
AGAGTAGCTGACAAAACTGC+AGG 0.579546 4.2:-51982570 None:intergenic
CCGGTAGCAACAACAAAGAT+GGG 0.583686 4.2:+51982694 MS.gene061320:CDS
GTTGGTGCAAAGGCAATGTA+GGG 0.584702 4.2:+51980987 MS.gene061320:CDS
CAATACTTGAAGAACAAGGA+AGG 0.589537 4.2:-51982296 None:intergenic
AATAACAATGGAAATATCAC+CGG 0.590268 4.2:+51982675 MS.gene061320:CDS
AAGTGTAATGGTTGGAAATG+GGG 0.590397 4.2:-51982337 None:intergenic
AATGGGAACAATGTCGCAGA+AGG 0.592519 4.2:+51982651 MS.gene061320:CDS
GTCGCAGAAGGTAATAACAA+TGG 0.594531 4.2:+51982663 MS.gene061320:CDS
ATGATGGAGAGTTGGTGCAA+AGG 0.595504 4.2:+51980977 MS.gene061320:CDS
TTCAAACTTAACAGGTACAA+AGG 0.596299 4.2:+51982097 MS.gene061320:intron
AAAAGACAAGAGAACTAAGA+GGG 0.601550 4.2:+51979408 MS.gene061320:CDS
AATACTTGAAGAACAAGGAA+GGG 0.605302 4.2:-51982295 None:intergenic
TATCGTTGCAGCATGGCCCG+TGG 0.614367 4.2:+51981409 MS.gene061320:CDS
TAGCAACAACAAAGATGGGA+AGG 0.617751 4.2:+51982698 MS.gene061320:CDS
TACATTAGATGAGATTGGAG+AGG 0.619862 4.2:-51979023 None:intergenic
TGGAGGACCTAATATTGACT+AGG 0.620153 4.2:-51978988 None:intergenic
GAAAACCTATAAGTAAGGAT+CGG 0.622633 4.2:+51981073 MS.gene061320:CDS
AGCATATTATCGTTGCAGCA+TGG 0.623216 4.2:+51981402 MS.gene061320:CDS
ATACTTGAAGAACAAGGAAG+GGG 0.623529 4.2:-51982294 None:intergenic
TGGCTGCTGCAGGTAAAGGG+TGG 0.624702 4.2:-51982170 None:intergenic
AAGTAAGGATCGGTTAGGAT+CGG 0.628790 4.2:+51981083 MS.gene061320:CDS
ATGGTGGAGTACTAGTGCCA+AGG 0.642358 4.2:+51980752 MS.gene061320:CDS
TACTAATGTTGAGACTGTGA+TGG 0.650638 4.2:+51981023 MS.gene061320:CDS
TGTTAATGTTGAAAATGTTG+AGG 0.658284 4.2:+51981161 MS.gene061320:CDS
AATCTCCAAATACTCCACAA+AGG 0.678231 4.2:+51982370 MS.gene061320:CDS
TATGAATATGATCCTTGTCG+GGG 0.678269 4.2:-51979114 None:intergenic
AGACAAGAGAACTAAGAGGG+AGG 0.687195 4.2:+51979411 MS.gene061320:CDS
GGCGTTGAAAAGCATCGAGG+AGG 0.690185 4.2:+51981104 MS.gene061320:CDS
GTTGCAATACTTGAAGAACA+AGG 0.694121 4.2:-51982300 None:intergenic
ATCGGCGTTGAAAAGCATCG+AGG 0.773629 4.2:+51981101 MS.gene061320:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAGTAGAAAAAAATAAT+GGG - chr4.2:51979197-51979216 None:intergenic 10.0%
!! TAATAAGTAGAAAAAAATAA+TGG - chr4.2:51979198-51979217 None:intergenic 10.0%
!!! ATGCATATTTAAATTTTTAA+TGG - chr4.2:51981900-51981919 None:intergenic 10.0%
!!! ATTTTTTTCTTTCTTTTATT+TGG + chr4.2:51980041-51980060 MS.gene061320:intron 10.0%
!!! TTATTTTCTGAAATTATTAT+TGG + chr4.2:51980215-51980234 MS.gene061320:intron 10.0%
!! AATAATCGAATTCAATTTAA+TGG + chr4.2:51980434-51980453 MS.gene061320:intron 15.0%
!! ATTATCTTTGGTTAAAATAA+AGG - chr4.2:51979814-51979833 None:intergenic 15.0%
!! CTACTGATAATAATAATAAT+GGG + chr4.2:51982634-51982653 MS.gene061320:CDS 15.0%
!! GAAAACATAATTATTATCTT+TGG - chr4.2:51979826-51979845 None:intergenic 15.0%
!! TGAATAAATAAACTCAATTT+AGG - chr4.2:51980105-51980124 None:intergenic 15.0%
!! TTATCTTTGGTTAAAATAAA+GGG - chr4.2:51979813-51979832 None:intergenic 15.0%
!!! ATATATATAATTTTCTTGGT+TGG + chr4.2:51979688-51979707 MS.gene061320:intron 15.0%
!! AAAAAATTAAAATGGAAGTG+AGG - chr4.2:51979631-51979650 None:intergenic 20.0%
!! AAAAATTAAAATGGAAGTGA+GGG - chr4.2:51979630-51979649 None:intergenic 20.0%
!! AAACATCATCACATAAAAAA+TGG - chr4.2:51980515-51980534 None:intergenic 20.0%
!! AATTATTTAGTGTACTTTGT+TGG - chr4.2:51980013-51980032 None:intergenic 20.0%
!! ATATTTGTTTCACAAATAAG+AGG - chr4.2:51980324-51980343 None:intergenic 20.0%
!! GCTACTGATAATAATAATAA+TGG + chr4.2:51982633-51982652 MS.gene061320:CDS 20.0%
!! TAGTTAAAATACTAATGAAG+TGG + chr4.2:51982041-51982060 MS.gene061320:intron 20.0%
!! TATCTTTGGTTAAAATAAAG+GGG - chr4.2:51979812-51979831 None:intergenic 20.0%
!! TGTACTATACTTATGAATAT+AGG - chr4.2:51979750-51979769 None:intergenic 20.0%
!!! AAAAGAAAAGAAGTTTTTGA+TGG + chr4.2:51980700-51980719 MS.gene061320:CDS 20.0%
!!! AATAATGGGATATTGAATTA+TGG - chr4.2:51979183-51979202 None:intergenic 20.0%
!!! AATAGAGTTTGATTTCTTTA+AGG + chr4.2:51979051-51979070 MS.gene061320:CDS 20.0%
!!! ATTTTTTTCCTTCCATTTAT+TGG + chr4.2:51979483-51979502 MS.gene061320:intron 20.0%
!!! TTATGATCATCTTGATAATT+TGG + chr4.2:51979284-51979303 MS.gene061320:intron 20.0%
!!! TTTAAATATGCATTGATGTT+TGG + chr4.2:51981907-51981926 MS.gene061320:intron 20.0%
! AAAAAGAGAATTGAGAATGA+TGG + chr4.2:51980961-51980980 MS.gene061320:CDS 25.0%
! AAGATAATGATTAAAGACCA+AGG - chr4.2:51981664-51981683 None:intergenic 25.0%
! AATAACAATGGAAATATCAC+CGG + chr4.2:51982675-51982694 MS.gene061320:CDS 25.0%
! AATAAGGTTATGGAAGTTAA+AGG + chr4.2:51980802-51980821 MS.gene061320:CDS 25.0%
! AATGAGAAAACCTATAAGTA+AGG + chr4.2:51981068-51981087 MS.gene061320:CDS 25.0%
! ACACTTTAACATACTTACAA+TGG - chr4.2:51981235-51981254 None:intergenic 25.0%
! ATTCATTTAACTGTTTCAGA+TGG + chr4.2:51980537-51980556 MS.gene061320:intron 25.0%
! TAAGTTCATTAAATTCAGCT+AGG - chr4.2:51979451-51979470 None:intergenic 25.0%
! TAGAACACTATTAACATGAT+GGG + chr4.2:51980388-51980407 MS.gene061320:intron 25.0%
! TGTTAATGTTGAAAATGTTG+AGG + chr4.2:51981161-51981180 MS.gene061320:CDS 25.0%
! TTAGAACACTATTAACATGA+TGG + chr4.2:51980387-51980406 MS.gene061320:intron 25.0%
! TTGAGTATAATCAAGTGTAA+TGG - chr4.2:51982352-51982371 None:intergenic 25.0%
!! CATCACATAAAAAATGGATT+TGG - chr4.2:51980509-51980528 None:intergenic 25.0%
!! GAAGTTTATGATGGAAAATT+TGG + chr4.2:51980829-51980848 MS.gene061320:CDS 25.0%
!!! AATTTTGATTGGTTCTGATT+AGG - chr4.2:51982471-51982490 None:intergenic 25.0%
!!! ATTTTGATCCAATAAATGGA+AGG - chr4.2:51979494-51979513 None:intergenic 25.0%
!!! ATTTTTTTTCTTCAACAGCT+TGG + chr4.2:51979316-51979335 MS.gene061320:intron 25.0%
!!! CTCAATTTTGATCCAATAAA+TGG - chr4.2:51979498-51979517 None:intergenic 25.0%
!!! GAATATTTTTATGTGCGATT+TGG + chr4.2:51980296-51980315 MS.gene061320:intron 25.0%
!!! TATAATTTTCTTGGTTGGTT+TGG + chr4.2:51979693-51979712 MS.gene061320:intron 25.0%
AAAAAAATAGCTGAGAATGG+TGG + chr4.2:51980736-51980755 MS.gene061320:CDS 30.0%
AAAAAGAGAATGAAGAATGG+TGG + chr4.2:51980856-51980875 MS.gene061320:CDS 30.0%
AAAAGACAAGAGAACTAAGA+GGG + chr4.2:51979408-51979427 MS.gene061320:CDS 30.0%
AAAGGACATGAAGTTTATGA+TGG + chr4.2:51980820-51980839 MS.gene061320:CDS 30.0%
AATACTTGAAGAACAAGGAA+GGG - chr4.2:51982298-51982317 None:intergenic 30.0%
ACTATGAATATGATCCTTGT+CGG - chr4.2:51979119-51979138 None:intergenic 30.0%
AGATTGATCACTACTAGTAT+TGG - chr4.2:51979359-51979378 None:intergenic 30.0%
ATTCCCATAGACAACAATAT+GGG + chr4.2:51979598-51979617 MS.gene061320:intron 30.0%
CAACAAACTCAAATCCAAAA+TGG + chr4.2:51982001-51982020 MS.gene061320:intron 30.0%
CACTATAGTTCAAACTTAAC+AGG + chr4.2:51982089-51982108 MS.gene061320:intron 30.0%
CATCCAATGATATGTTATCA+CGG + chr4.2:51979897-51979916 MS.gene061320:intron 30.0%
CTTGTTACATTAGATGAGAT+TGG - chr4.2:51979031-51979050 None:intergenic 30.0%
GAAAACCTATAAGTAAGGAT+CGG + chr4.2:51981073-51981092 MS.gene061320:CDS 30.0%
GAAAATTATGGATCTTGGAT+CGG + chr4.2:51980774-51980793 MS.gene061320:CDS 30.0%
GAGAAAAAAATAGCTGAGAA+TGG + chr4.2:51980733-51980752 MS.gene061320:CDS 30.0%
GAGAAAAAGAGAATGAAGAA+TGG + chr4.2:51980853-51980872 MS.gene061320:CDS 30.0%
GTATAATCAAGTGTAATGGT+TGG - chr4.2:51982348-51982367 None:intergenic 30.0%
GTTAAAATAAAGGGGAGAAA+TGG - chr4.2:51979804-51979823 None:intergenic 30.0%
TAACATCCTCATTCTTACTT+AGG - chr4.2:51981146-51981165 None:intergenic 30.0%
TAATGCGAAAGCAATCATTA+AGG - chr4.2:51981515-51981534 None:intergenic 30.0%
TAGTAGTGATCAATCTATGA+TGG + chr4.2:51979363-51979382 MS.gene061320:CDS 30.0%
TAGTGATACATCATCTTTGT+TGG + chr4.2:51979135-51979154 MS.gene061320:CDS 30.0%
TCAGCAAAAACAAAATGCAT+TGG - chr4.2:51980415-51980434 None:intergenic 30.0%
TTACCGTGATAACATATCAT+TGG - chr4.2:51979903-51979922 None:intergenic 30.0%
TTATTGTGTTAGATCACTGA+TGG + chr4.2:51981325-51981344 MS.gene061320:intron 30.0%
TTCAAACTTAACAGGTACAA+AGG + chr4.2:51982097-51982116 MS.gene061320:intron 30.0%
! ACCTAATATTGACTAGGAAA+GGG - chr4.2:51978985-51979004 None:intergenic 30.0%
! AGAAGAAAGTTACCTGTTTT+CGG - chr4.2:51981444-51981463 None:intergenic 30.0%
! CATGTTTCTATGCTAGAAAA+TGG + chr4.2:51981818-51981837 MS.gene061320:intron 30.0%
! GTTCTCTTGTCTTTTGAATT+AGG - chr4.2:51979403-51979422 None:intergenic 30.0%
! TATAGGTTTTCTCATTGATG+AGG - chr4.2:51981064-51981083 None:intergenic 30.0%
! TCTATGCTAGAAAATGGATT+TGG + chr4.2:51981824-51981843 MS.gene061320:intron 30.0%
!! AAAAGGAAATTTGTTGATGC+TGG + chr4.2:51980889-51980908 MS.gene061320:CDS 30.0%
!! AAAATTTGGATTAGCAGCAA+TGG - chr4.2:51982550-51982569 None:intergenic 30.0%
!! AAAGGAAATTTGTTGATGCT+GGG + chr4.2:51980890-51980909 MS.gene061320:CDS 30.0%
!! AAGGGACACAAAATTTTGAT+TGG - chr4.2:51982482-51982501 None:intergenic 30.0%
!! AAGGTCAAAGAAGTGTTTAA+TGG + chr4.2:51980925-51980944 MS.gene061320:CDS 30.0%
!! GTTTTGATATACATTGCACA+AGG + chr4.2:51981747-51981766 MS.gene061320:intron 30.0%
!! TGTCTATATTTGATAATGCC+TGG + chr4.2:51979542-51979561 MS.gene061320:intron 30.0%
!!! ACATGCATATTTTAAGACCT+TGG + chr4.2:51981644-51981663 MS.gene061320:intron 30.0%
!!! AGTTGTTTTTGCCATTTCTT+TGG - chr4.2:51982193-51982212 None:intergenic 30.0%
!!! GTATTTTAACTAACCTAGCT+AGG - chr4.2:51982033-51982052 None:intergenic 30.0%
AAGTGTAATGGTTGGAAATG+GGG - chr4.2:51982340-51982359 None:intergenic 35.0%
AATCTCCAAATACTCCACAA+AGG + chr4.2:51982370-51982389 MS.gene061320:CDS 35.0%
ACATCAACATCAAGCAATGA+TGG + chr4.2:51982240-51982259 MS.gene061320:CDS 35.0%
ACTAGTGCCAAGGAAAATTA+TGG + chr4.2:51980762-51980781 MS.gene061320:CDS 35.0%
ACTGACATACACATAAACAC+TGG - chr4.2:51980269-51980288 None:intergenic 35.0%
AGGAATTTGCAAAAGTCTGA+CGG - chr4.2:51982425-51982444 None:intergenic 35.0%
ATACTTGAAGAACAAGGAAG+GGG - chr4.2:51982297-51982316 None:intergenic 35.0%
ATCGGCTACTAATAAGGTTA+TGG + chr4.2:51980792-51980811 MS.gene061320:CDS 35.0%
ATTCGAGTGTCACACATTAA+TGG - chr4.2:51979570-51979589 None:intergenic 35.0%
CAAAAGACAAGAGAACTAAG+AGG + chr4.2:51979407-51979426 MS.gene061320:CDS 35.0%
CAAGTGTAATGGTTGGAAAT+GGG - chr4.2:51982341-51982360 None:intergenic 35.0%
CAATACTTGAAGAACAAGGA+AGG - chr4.2:51982299-51982318 None:intergenic 35.0%
CCAAGGAAAATTATGGATCT+TGG + chr4.2:51980769-51980788 MS.gene061320:CDS 35.0%
CCGGAAAATTTGAGTAGTAA+GGG - chr4.2:51980143-51980162 None:intergenic 35.0%
CGTGATAACATATCATTGGA+TGG - chr4.2:51979899-51979918 None:intergenic 35.0%
CTATGAATATGATCCTTGTC+GGG - chr4.2:51979118-51979137 None:intergenic 35.0%
CTGCAACGATAATATGCTTT+AGG - chr4.2:51981400-51981419 None:intergenic 35.0%
GAAAGTGATGCAAGATTGTA+AGG + chr4.2:51980666-51980685 MS.gene061320:CDS 35.0%
GACAAAACTGCAGGAAAATT+TGG - chr4.2:51982564-51982583 None:intergenic 35.0%
GATGCAAGATTGTAAGGTTA+AGG + chr4.2:51980672-51980691 MS.gene061320:CDS 35.0%
GATTCCCATAGACAACAATA+TGG + chr4.2:51979597-51979616 MS.gene061320:intron 35.0%
GGATGTCAATGAAGAAAGTA+TGG + chr4.2:51981346-51981365 MS.gene061320:intron 35.0%
GTTAGTCCTAAGTAAGAATG+AGG + chr4.2:51981137-51981156 MS.gene061320:CDS 35.0%
GTTGCAATACTTGAAGAACA+AGG - chr4.2:51982303-51982322 None:intergenic 35.0%
GTTGTCATCACAACATATGA+AGG + chr4.2:51982138-51982157 MS.gene061320:CDS 35.0%
GTTTCTGTCCGTATCATATA+GGG - chr4.2:51980178-51980197 None:intergenic 35.0%
TAATGTGTGACACTCGAATA+GGG + chr4.2:51979571-51979590 MS.gene061320:intron 35.0%
TACATTAGATGAGATTGGAG+AGG - chr4.2:51979026-51979045 None:intergenic 35.0%
TACTAATGTTGAGACTGTGA+TGG + chr4.2:51981023-51981042 MS.gene061320:CDS 35.0%
TATCATATAGGGGCAATGAA+GGG - chr4.2:51980167-51980186 None:intergenic 35.0%
TATGAATATGATCCTTGTCG+GGG - chr4.2:51979117-51979136 None:intergenic 35.0%
TCAAGTGTAATGGTTGGAAA+TGG - chr4.2:51982342-51982361 None:intergenic 35.0%
TCCCTTTCCTAGTCAATATT+AGG + chr4.2:51978981-51979000 MS.gene061320:CDS 35.0%
TTAATGTGTGACACTCGAAT+AGG + chr4.2:51979570-51979589 MS.gene061320:intron 35.0%
TTCATTGCCCCTATATGATA+CGG + chr4.2:51980167-51980186 MS.gene061320:intron 35.0%
TTCGAGTGTCACACATTAAT+GGG - chr4.2:51979569-51979588 None:intergenic 35.0%
TTGATGTAGCAACACATCAT+TGG + chr4.2:51979963-51979982 MS.gene061320:intron 35.0%
TTGTGTCCCTTCAAAACTAT+TGG + chr4.2:51982491-51982510 MS.gene061320:CDS 35.0%
TTTCAGATGGCTATTCTTCA+AGG + chr4.2:51980550-51980569 MS.gene061320:intron 35.0%
TTTCTGTCCGTATCATATAG+GGG - chr4.2:51980177-51980196 None:intergenic 35.0%
! AAAGGAGTTTGAAACTGATC+TGG - chr4.2:51982399-51982418 None:intergenic 35.0%
! AAGGAGTTTGAAACTGATCT+GGG - chr4.2:51982398-51982417 None:intergenic 35.0%
! AATTGAGAATGATGGAGAGT+TGG + chr4.2:51980969-51980988 MS.gene061320:CDS 35.0%
! ATGGTGGAGAATTAGTGAAA+AGG + chr4.2:51980872-51980891 MS.gene061320:CDS 35.0%
! CCAAGATCCATAATTTTCCT+TGG - chr4.2:51980772-51980791 None:intergenic 35.0%
! CCCTTACTACTCAAATTTTC+CGG + chr4.2:51980140-51980159 MS.gene061320:intron 35.0%
! CCTTACTACTCAAATTTTCC+GGG + chr4.2:51980141-51980160 MS.gene061320:intron 35.0%
! GACCTAATATTGACTAGGAA+AGG - chr4.2:51978986-51979005 None:intergenic 35.0%
! GTATTGGCAGTAACATGATT+GGG - chr4.2:51979343-51979362 None:intergenic 35.0%
! TAAATGCATGGATTGCAGAA+TGG - chr4.2:51981857-51981876 None:intergenic 35.0%
! TGTTTCTGTCCGTATCATAT+AGG - chr4.2:51980179-51980198 None:intergenic 35.0%
!! ACGTTTTATTCGCATGTGAT+GGG + chr4.2:51980481-51980500 MS.gene061320:intron 35.0%
!! AGAAACATGAGCTTTGAAAG+TGG - chr4.2:51981807-51981826 None:intergenic 35.0%
!! AGTATTGGCAGTAACATGAT+TGG - chr4.2:51979344-51979363 None:intergenic 35.0%
!! CGATAATATGCTTTAGGACA+CGG - chr4.2:51981394-51981413 None:intergenic 35.0%
!! GATAATATGCTTTAGGACAC+GGG - chr4.2:51981393-51981412 None:intergenic 35.0%
!!! GTACTGCCAATAGTTTTGAA+GGG - chr4.2:51982500-51982519 None:intergenic 35.0%
AAAATGGTAGCTACCTAGCT+AGG + chr4.2:51982017-51982036 MS.gene061320:intron 40.0%
AAGTAAGGATCGGTTAGGAT+CGG + chr4.2:51981083-51981102 MS.gene061320:CDS 40.0%
ACATGCGAATAAAACGTGTC+AGG - chr4.2:51980478-51980497 None:intergenic 40.0%
AGCATATTATCGTTGCAGCA+TGG + chr4.2:51981402-51981421 MS.gene061320:CDS 40.0%
ATGGTCGTTGTATCGAAGAT+TGG - chr4.2:51981938-51981957 None:intergenic 40.0%
CATGCGAATAAAACGTGTCA+GGG - chr4.2:51980477-51980496 None:intergenic 40.0%
CCATATAGTAAGTACGTCGA+TGG - chr4.2:51981957-51981976 None:intergenic 40.0%
CCATCGACGTACTTACTATA+TGG + chr4.2:51981954-51981973 MS.gene061320:intron 40.0%
CCCGGAAAATTTGAGTAGTA+AGG - chr4.2:51980144-51980163 None:intergenic 40.0%
CCTAACCGATCCTTACTTAT+AGG - chr4.2:51981081-51981100 None:intergenic 40.0%
CCTATAAGTAAGGATCGGTT+AGG + chr4.2:51981078-51981097 MS.gene061320:CDS 40.0%
CGACGTACTTACTATATGGA+TGG + chr4.2:51981958-51981977 MS.gene061320:intron 40.0%
CGAGCTTGAACGAAAGAAAA+CGG + chr4.2:51980573-51980592 MS.gene061320:CDS 40.0%
GCTACTCTTACTTCCATGAT+TGG + chr4.2:51982585-51982604 MS.gene061320:CDS 40.0%
GGAGAAATGGATGACACTAA+TGG - chr4.2:51979791-51979810 None:intergenic 40.0%
GTATCATATAGGGGCAATGA+AGG - chr4.2:51980168-51980187 None:intergenic 40.0%
GTCGCAGAAGGTAATAACAA+TGG + chr4.2:51982663-51982682 MS.gene061320:CDS 40.0%
TAGATGAGATTGGAGAGGTT+TGG - chr4.2:51979021-51979040 None:intergenic 40.0%
TAGCAACAACAAAGATGGGA+AGG + chr4.2:51982698-51982717 MS.gene061320:CDS 40.0%
TCTTGGATCGGCTACTAATA+AGG + chr4.2:51980786-51980805 MS.gene061320:CDS 40.0%
TGGCCCATATTGTTGTCTAT+GGG - chr4.2:51979604-51979623 None:intergenic 40.0%
TTGACTGCCTGAGCAATATT+TGG - chr4.2:51982522-51982541 None:intergenic 40.0%
TTGGCCCATATTGTTGTCTA+TGG - chr4.2:51979605-51979624 None:intergenic 40.0%
! TAAAATGGAAGTGAGGGCAT+TGG - chr4.2:51979624-51979643 None:intergenic 40.0%
! TAGCTAGGTAGCTACCATTT+TGG - chr4.2:51982018-51982037 None:intergenic 40.0%
! TGGAGGACCTAATATTGACT+AGG - chr4.2:51978991-51979010 None:intergenic 40.0%
!! CACGTTTTATTCGCATGTGA+TGG + chr4.2:51980480-51980499 MS.gene061320:intron 40.0%
!! GAAATTTGTTGATGCTGGGT+TGG + chr4.2:51980894-51980913 MS.gene061320:CDS 40.0%
!! GCAAAAGTCTGACGGATTAA+AGG - chr4.2:51982417-51982436 None:intergenic 40.0%
!! GTTGGTTTGGTTTGTAGCTT+TGG + chr4.2:51979706-51979725 MS.gene061320:intron 40.0%
!!! AGAGGTTTGGTTTTCATTGG+AGG - chr4.2:51979008-51979027 None:intergenic 40.0%
!!! GGTACTGCCAATAGTTTTGA+AGG - chr4.2:51982501-51982520 None:intergenic 40.0%
!!! TGGAGAGGTTTGGTTTTCAT+TGG - chr4.2:51979011-51979030 None:intergenic 40.0%
AACTGATCTGGGTTCCTTTG+TGG - chr4.2:51982387-51982406 None:intergenic 45.0%
AATGGGAACAATGTCGCAGA+AGG + chr4.2:51982651-51982670 MS.gene061320:CDS 45.0%
ACCGGTAGCAACAACAAAGA+TGG + chr4.2:51982693-51982712 MS.gene061320:CDS 45.0%
AGACAAGAGAACTAAGAGGG+AGG + chr4.2:51979411-51979430 MS.gene061320:CDS 45.0%
AGAGTAGCTGACAAAACTGC+AGG - chr4.2:51982573-51982592 None:intergenic 45.0%
AGGCAGAGACAACTTCTTGA+TGG - chr4.2:51979090-51979109 None:intergenic 45.0%
CCGGTAGCAACAACAAAGAT+GGG + chr4.2:51982694-51982713 MS.gene061320:CDS 45.0%
GGCAGTACCAAATATTGCTC+AGG + chr4.2:51982512-51982531 MS.gene061320:CDS 45.0%
GTTGGTGCAAAGGCAATGTA+GGG + chr4.2:51980987-51981006 MS.gene061320:CDS 45.0%
TTGAGAGATCAGAGAAGCTG+AGG - chr4.2:51982445-51982464 None:intergenic 45.0%
TTTAATGGCCATCGACGTAC+TGG - chr4.2:51981885-51981904 None:intergenic 45.0%
! AGTTGGTGCAAAGGCAATGT+AGG + chr4.2:51980986-51981005 MS.gene061320:CDS 45.0%
! GGGTTCCTTTGTGGAGTATT+TGG - chr4.2:51982378-51982397 None:intergenic 45.0%
! TCAGTAGCAGCAGTGTTGTT+TGG - chr4.2:51982621-51982640 None:intergenic 45.0%
! TGTCACACATTAATGGGTCC+AGG - chr4.2:51979563-51979582 None:intergenic 45.0%
!! ATGATGGAGAGTTGGTGCAA+AGG + chr4.2:51980977-51980996 MS.gene061320:CDS 45.0%
!! CCCATCTTTGTTGTTGCTAC+CGG - chr4.2:51982697-51982716 None:intergenic 45.0%
!! TGCTGGGTTGGCTACTAATA+AGG + chr4.2:51980906-51980925 MS.gene061320:CDS 45.0%
!! TAAAATATATGTATTTATTA+TGG + chr4.2:51981473-51981492 MS.gene061320:intron 5.0%
!! TATATTTGAAAAAATTAAAA+TGG - chr4.2:51979639-51979658 None:intergenic 5.0%
!!! ATATATATATATAATTTTCT+TGG + chr4.2:51979684-51979703 MS.gene061320:intron 5.0%
ACCTGCAGCAGCCAAAGAAA+TGG + chr4.2:51982179-51982198 MS.gene061320:CDS 50.0%
CATGTCTGTGACTGCGATCT+AGG - chr4.2:51981629-51981648 None:intergenic 50.0%
CTTTGGCTGCTGCAGGTAAA+GGG - chr4.2:51982176-51982195 None:intergenic 50.0%
GTGTGACACTCGAATAGGGA+TGG + chr4.2:51979575-51979594 MS.gene061320:intron 50.0%
TCTTTGGCTGCTGCAGGTAA+AGG - chr4.2:51982177-51982196 None:intergenic 50.0%
TGGTTGCTCAGTCCGAAAAC+AGG + chr4.2:51981429-51981448 MS.gene061320:CDS 50.0%
! ATGGTGGAGTACTAGTGCCA+AGG + chr4.2:51980752-51980771 MS.gene061320:CDS 50.0%
! GTACTGGCAGCGTAAATGCA+TGG - chr4.2:51981869-51981888 None:intergenic 50.0%
! GTTTTCGGACTGAGCAACCA+CGG - chr4.2:51981429-51981448 None:intergenic 50.0%
! TGGCTGAGTATCGCCAATCA+TGG - chr4.2:51982601-51982620 None:intergenic 50.0%
! TTTTCGGACTGAGCAACCAC+GGG - chr4.2:51981428-51981447 None:intergenic 50.0%
!! AAGCATCGAGGAGGCTTTGA+AGG + chr4.2:51981113-51981132 MS.gene061320:CDS 50.0%
!! ATCGGCGTTGAAAAGCATCG+AGG + chr4.2:51981101-51981120 MS.gene061320:CDS 50.0%
ATGATCCTTGTCGGGGACAG+AGG - chr4.2:51979110-51979129 None:intergenic 55.0%
GCCATTTCTTTGGCTGCTGC+AGG - chr4.2:51982183-51982202 None:intergenic 55.0%
TATAGGGGCAATGAAGGGCC+CGG - chr4.2:51980162-51980181 None:intergenic 55.0%
TTACGCTGCCAGTACGTCGA+TGG + chr4.2:51981874-51981893 MS.gene061320:intron 55.0%
!! GGCGTTGAAAAGCATCGAGG+AGG + chr4.2:51981104-51981123 MS.gene061320:CDS 55.0%
TATCGTTGCAGCATGGCCCG+TGG + chr4.2:51981409-51981428 MS.gene061320:CDS 60.0%
TGGCTGCTGCAGGTAAAGGG+TGG - chr4.2:51982173-51982192 None:intergenic 60.0%
CTCTGCCTCTGTCCCCGACA+AGG + chr4.2:51979102-51979121 MS.gene061320:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 51978959 51982725 51978959 ID=MS.gene061320
chr4.2 mRNA 51978959 51982725 51978959 ID=MS.gene061320.t1;Parent=MS.gene061320
chr4.2 exon 51978959 51979171 51978959 ID=MS.gene061320.t1.exon1;Parent=MS.gene061320.t1
chr4.2 CDS 51978959 51979171 51978959 ID=cds.MS.gene061320.t1;Parent=MS.gene061320.t1
chr4.2 exon 51979334 51979432 51979334 ID=MS.gene061320.t1.exon2;Parent=MS.gene061320.t1
chr4.2 CDS 51979334 51979432 51979334 ID=cds.MS.gene061320.t1;Parent=MS.gene061320.t1
chr4.2 exon 51980556 51981236 51980556 ID=MS.gene061320.t1.exon3;Parent=MS.gene061320.t1
chr4.2 CDS 51980556 51981236 51980556 ID=cds.MS.gene061320.t1;Parent=MS.gene061320.t1
chr4.2 exon 51981364 51981450 51981364 ID=MS.gene061320.t1.exon4;Parent=MS.gene061320.t1
chr4.2 CDS 51981364 51981450 51981364 ID=cds.MS.gene061320.t1;Parent=MS.gene061320.t1
chr4.2 exon 51982111 51982725 51982111 ID=MS.gene061320.t1.exon5;Parent=MS.gene061320.t1
chr4.2 CDS 51982111 51982725 51982111 ID=cds.MS.gene061320.t1;Parent=MS.gene061320.t1
Gene Sequence

>MS.gene061320

ATGGATGCTACACATAGTACTATCCCTTTCCTAGTCAATATTAGGTCCTCCAATGAAAACCAAACCTCTCCAATCTCATCTAATGTAACAAGAATAGAGTTTGATTTCTTTAAGGACAACAACAATGACCATCAAGAAGTTGTCTCTGCCTCTGTCCCCGACAAGGATCATATTCATAGTGATACATCATCTTTGTTGGAACTCAAGTTGAGTGTGAGTGACCATAATTCAATATCCCATTATTTTTTTCTACTTATTATATTAAATATATTGTTTTTAAATTTCATAGCTTTTGAATTTGTAATCGCAAACAATCATTTATTTTTTATGATCATCTTGATAATTTGGCGAATATGTATTTTTTTTCTTCAACAGCTTGGTCCCAATCATGTTACTGCCAATACTAGTAGTGATCAATCTATGATGGATGACAACATGTCACCTAATTCAAAAGACAAGAGAACTAAGAGGGAGGTAATGTTAATGACACCTAGCTGAATTTAATGAACTTATATCTAATGAACATTTTTTTCCTTCCATTTATTGGATCAAAATTGAGTGATTTTATATATAGAATCGTCAATGTCTATATTTGATAATGCCTGGACCCATTAATGTGTGACACTCGAATAGGGATGGATTCCCATAGACAACAATATGGGCCAATGCCCTCACTTCCATTTTAATTTTTTCAAATATATATATATATATATATATATATATATATATATATATATAATTTTCTTGGTTGGTTTGGTTTGTAGCTTTGGTCTCTTACTCTTCTATGACCTATATTCATAAGTATAGTACAATATAATAATATATGCAACCATTAGTGTCATCCATTTCTCCCCTTTATTTTAACCAAAGATAATAATTATGTTTTCATATTGAGTTTAACTAGTATGTTTTGTTAACGTACAGTAATTTAATACATCCATCCAATGATATGTTATCACGGTAATTAATGAATATCAAAACTCATGTGTTTTCATATTTATTAATTGATGTAGCAACACATCATTGGATGAACGTGTAAAATAATTGTACACCAACAAAGTACACTAAATAATTTCTTTCATATTTTTTTCTTTCTTTTATTTGGTTTCATTTTTTAAAACATACTTTGTTTAATATGTTAAACCTAAATTGAGTTTATTTATTCAATATTTTAAATACTTCCCTTACTACTCAAATTTTCCGGGCCCTTCATTGCCCCTATATGATACGGACAGAAACAATTACATTTTAGTGTTTTATTTTCTGAAATTATTATTGGTTGTTGAGTGTCAGTTTTATTGTTTTGTCCAGTGTTTATGTGTATGTCAGTGTTTATAGAATATTTTTATGTGCGATTTGGATCCTCTTATTTGTGAAACAAATATGTGAGTTATGTCACTAAACTCACTATTAAAATGCATTGCACTCTTAGAACACTATTAACATGATGGGTCCAATGCATTTTGTTTTTGCTGAATAATCGAATTCAATTTAATGGTGAAATGATTATTGTAACCCTGACACGTTTTATTCGCATGTGATGGGATCCAAATCCATTTTTTATGTGATGATGTTTGAATTCATTTAACTGTTTCAGATGGCTATTCTTCAAGGCGAGCTTGAACGAAAGAAAACGGAGAACTGTAGATTAAAATTGATGTATGATCAGCTTAGAACAGATTACAATTACATGCGAATGAATTTCGAGAAAGTGATGCAAGATTGTAAGGTTAAGGAAGTCAAAAGAAAAGAAGTTTTTGATGGAAAAATTAAAGAGAAAAAAATAGCTGAGAATGGTGGAGTACTAGTGCCAAGGAAAATTATGGATCTTGGATCGGCTACTAATAAGGTTATGGAAGTTAAAGGACATGAAGTTTATGATGGAAAATTTGGTGAGAAAAAGAGAATGAAGAATGGTGGAGAATTAGTGAAAAGGAAATTTGTTGATGCTGGGTTGGCTACTAATAAGGTCAAAGAAGTGTTTAATGGTAAATGTGAAAAGAAAAAGAGAATTGAGAATGATGGAGAGTTGGTGCAAAGGCAATGTAGGGATCTTGTGTTGATTACTAATGTTGAGACTGTGATGGATCATGAAGCTTCTTCCTCATCAATGAGAAAACCTATAAGTAAGGATCGGTTAGGATCGGCGTTGAAAAGCATCGAGGAGGCTTTGAAGGAGTTAGTCCTAAGTAAGAATGAGGATGTTAATGTTGAAAATGTTGAGGCTACATTGACAAAAGCAAGAGTTACTATTAGAGCTAGATCAGAAGAGACCATTGTAAGTATGTTAAAGTGTATATTAACTTTCAACTTTGCAAGATTTTATATAATAAATTGCTGTTCTTTTTCGCTAAAAGAAATTATTTTTATTGTGTTAGATCACTGATGGATGTCAATGAAGAAAGTATGGACAGAAACTGTCGAAAAGAAACCCGTGTCCTAAAGCATATTATCGTTGCAGCATGGCCCGTGGTTGCTCAGTCCGAAAACAGGTAACTTTCTTCTGTAGCTATCTAAAATATATGTATTTATTATGGTTTTAAATTTTTGTCACCTTAATGATTGCTTTCGCATTATTACATTCAACATTATATAGAATTACAATCAAATATGATTTATGCAACGTTTGCATTATAATCTTAATGACATTAAACTCTTTGATTGTTGCCTAGATCGCAGTCACAGACATGCATATTTTAAGACCTTGGTCTTTAATCATTATCTTTATCTATGTTTTCAACTACTTACAAAATTATTTTGCATAGATAATTTTTTTTAGTAATTGATTGTTTTGATATACATTGCACAAGGTTGAATAACAATAGAATTTAATTGAAATGTGTTTCCACTTTCAAAGCTCATGTTTCTATGCTAGAAAATGGATTTGGATTTGCTCCATTCTGCAATCCATGCATTTACGCTGCCAGTACGTCGATGGCCATTAAAAATTTAAATATGCATTGATGTTTGGATCATCCAATCTTCGATACAACGACCATCGACGTACTTACTATATGGATGGATGCATGAATTTTCTGACTGCAACAAACTCAAATCCAAAATGGTAGCTACCTAGCTAGGTTAGTTAAAATACTAATGAAGTGGTATGTATTATAATGACAATATTATTCACTATAGTTCAAACTTAACAGGTACAAAGGTGTGCTCTTGACAGAGCAGTTGTCATCACAACATATGAAGGAAATCACAACCACCCTTTACCTGCAGCAGCCAAAGAAATGGCAAAAACAACTTCAGCAGCTGCAAAGATTCTACTTTCAACATCAACATCAAGCAATGATGGACAATTGAATGCAAATTTACTCAGAAGAACACCCCTTCCTTGTTCTTCAAGTATTGCAACTATCTCAACTTCTGCCCCATTTCCAACCATTACACTTGATTATACTCAATCTCCAAATACTCCACAAAGGAACCCAGATCAGTTTCAAACTCCTTTAATCCGTCAGACTTTTGCAAATTCCTCAGCTTCTCTGATCTCTCAAACACCTAATCAGAACCAATCAAAATTTTGTGTCCCTTCAAAACTATTGGCAGTACCAAATATTGCTCAGGCAGTCAATGCTGCCATTGCTGCTAATCCAAATTTTCCTGCAGTTTTGTCAGCTACTCTTACTTCCATGATTGGCGATACTCAGCCAAACAACACTGCTGCTACTGATAATAATAATAATGGGAACAATGTCGCAGAAGGTAATAACAATGGAAATATCACCGGTAGCAACAACAAAGATGGGAAGGAATAA
Protein sequence

>MS.gene061320.t1

MDATHSTIPFLVNIRSSNENQTSPISSNVTRIEFDFFKDNNNDHQEVVSASVPDKDHIHSDTSSLLELKLSLGPNHVTANTSSDQSMMDDNMSPNSKDKRTKREMAILQGELERKKTENCRLKLMYDQLRTDYNYMRMNFEKVMQDCKVKEVKRKEVFDGKIKEKKIAENGGVLVPRKIMDLGSATNKVMEVKGHEVYDGKFGEKKRMKNGGELVKRKFVDAGLATNKVKEVFNGKCEKKKRIENDGELVQRQCRDLVLITNVETVMDHEASSSSMRKPISKDRLGSALKSIEEALKELVLSKNEDVNVENVEATLTKARVTIRARSEETIYGQKLSKRNPCPKAYYRCSMARGCSVRKQVQRCALDRAVVITTYEGNHNHPLPAAAKEMAKTTSAAAKILLSTSTSSNDGQLNANLLRRTPLPCSSSIATISTSAPFPTITLDYTQSPNTPQRNPDQFQTPLIRQTFANSSASLISQTPNQNQSKFCVPSKLLAVPNIAQAVNAAIAANPNFPAVLSATLTSMIGDTQPNNTAATDNNNNGNNVAEGNNNGNITGSNNKDGKE