AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061342

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061342.t1 MTR_8g006705 96.732 306 6 2 1 303 1 305 0.0 603
MS.gene061342.t1 MTR_5g013010 58.958 307 96 11 3 301 4 288 2.80e-99 294
MS.gene061342.t1 MTR_4g100550 57.627 295 92 9 1 294 3 265 3.46e-95 283
MS.gene061342.t1 MTR_7g093430 66.851 181 45 4 81 257 104 273 1.65e-70 220
MS.gene061342.t1 MTR_1g054285 70.625 160 28 4 100 259 129 269 1.66e-67 213
MS.gene061342.t1 MTR_2g061030 74.648 142 31 2 126 267 187 323 8.51e-66 209
MS.gene061342.t1 MTR_4g097600 74.809 131 27 2 126 256 115 239 6.07e-64 202
MS.gene061342.t1 MTR_5g014890 76.667 120 27 1 137 256 117 235 4.64e-62 198
MS.gene061342.t1 MTR_1g017090 54.913 173 64 6 88 257 84 245 6.72e-53 175
MS.gene061342.t1 MTR_4g084750 63.710 124 40 1 137 255 59 182 9.32e-50 164
MS.gene061342.t1 MTR_5g013010 52.261 199 73 8 3 194 4 187 1.29e-45 153
MS.gene061342.t1 MTR_2g038625 57.031 128 48 1 129 256 75 195 6.37e-43 147
MS.gene061342.t1 MTR_2g038580 57.031 128 48 1 129 256 75 195 6.37e-43 147
MS.gene061342.t1 MTR_3g103590 64.516 124 42 2 142 265 144 265 8.44e-43 149
MS.gene061342.t1 MTR_4g126900 62.281 114 40 1 145 255 90 203 1.14e-37 133
MS.gene061342.t1 MTR_6g007647 45.631 103 54 1 140 240 30 132 2.94e-19 85.5
MS.gene061342.t1 MTR_6g007647 43.519 108 61 0 140 247 64 171 3.02e-19 86.3
MS.gene061342.t1 MTR_7g010020 47.222 108 48 2 129 232 71 173 4.11e-19 85.5
MS.gene061342.t1 MTR_6g011610 34.615 156 100 1 77 230 9 164 4.44e-19 85.1
MS.gene061342.t1 MTR_5g039000 47.059 102 48 1 129 230 69 164 5.79e-19 85.1
MS.gene061342.t1 MTR_6g011610 40.164 122 73 0 109 230 62 183 1.86e-18 83.6
MS.gene061342.t1 MTR_4g107650 45.690 116 61 1 117 230 67 182 3.03e-18 84.0
MS.gene061342.t1 MTR_8g468210 45.545 101 49 1 129 229 69 163 3.81e-18 82.8
MS.gene061342.t1 MTR_5g038280 48.515 101 44 2 143 236 87 186 9.65e-18 82.4
MS.gene061342.t1 MTR_1g061660 44.681 94 51 1 138 231 55 147 2.87e-17 79.7
MS.gene061342.t1 MTR_3g080100 38.571 140 81 2 143 278 20 158 6.70e-17 78.2
MS.gene061342.t1 MTR_7g103340 37.410 139 83 3 137 272 56 193 8.03e-17 78.2
MS.gene061342.t1 MTR_3g086790 40.152 132 68 4 133 254 49 179 9.76e-17 79.3
MS.gene061342.t1 MTR_3g080100 38.571 140 81 2 143 278 59 197 1.34e-16 78.2
MS.gene061342.t1 MTR_8g089895 37.584 149 80 4 130 271 43 185 1.56e-16 79.0
MS.gene061342.t1 MTR_8g469430 46.809 94 43 1 143 229 89 182 9.16e-16 76.3
MS.gene061342.t1 MTR_5g019650 42.593 108 59 2 132 237 43 149 1.33e-12 66.2
MS.gene061342.t1 MTR_5g019680 41.667 108 57 2 130 237 41 142 1.84e-12 65.5
MS.gene061342.t1 MTR_3g092150 41.935 93 47 1 146 231 41 133 2.33e-11 63.2
MS.gene061342.t1 MTR_8g026960 33.333 129 73 2 147 275 38 153 7.44e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061342.t1 AT3G60390 59.797 296 92 8 16 300 30 309 4.43e-104 306
MS.gene061342.t1 AT5G47370 53.871 310 88 13 1 298 3 269 5.56e-90 270
MS.gene061342.t1 AT2G44910 55.352 327 109 12 1 301 1 316 9.89e-88 265
MS.gene061342.t1 AT4G16780 55.629 302 88 9 1 293 2 266 3.73e-84 255
MS.gene061342.t1 AT4G17460 52.632 304 100 10 1 296 3 270 1.03e-83 254
MS.gene061342.t1 AT5G06710 63.235 204 58 6 87 281 134 329 2.71e-73 229
MS.gene061342.t1 AT4G37790 60.795 176 59 3 117 292 106 271 1.13e-65 207
MS.gene061342.t1 AT2G22800 61.111 162 56 3 137 292 107 267 3.02e-61 196
MS.gene061342.t1 AT2G01430 49.223 193 85 5 88 278 88 269 1.92e-50 168
MS.gene061342.t1 AT1G70920 55.782 147 49 3 127 271 29 161 1.45e-41 142
MS.gene061342.t1 AT1G70920 55.782 147 49 3 127 271 59 191 2.63e-41 142
MS.gene061342.t1 AT5G06710 59.649 114 32 4 88 194 135 241 6.74e-30 114
MS.gene061342.t1 AT1G69780 44.118 136 66 2 95 230 47 172 7.06e-22 93.6
MS.gene061342.t1 AT2G01430 53.846 91 39 2 106 194 101 190 8.01e-22 91.7
MS.gene061342.t1 AT1G26960 44.444 117 56 2 117 230 48 158 5.31e-19 84.7
MS.gene061342.t1 AT3G01470 48.387 93 41 1 143 228 68 160 1.48e-17 81.3
MS.gene061342.t1 AT5G65310 42.478 113 58 1 125 230 54 166 4.60e-17 80.5
MS.gene061342.t1 AT5G65310 42.478 113 58 1 125 230 36 148 5.08e-17 80.1
MS.gene061342.t1 AT5G15150 47.872 94 45 1 143 232 115 208 5.03e-16 77.4
MS.gene061342.t1 AT3G01220 45.745 94 47 1 143 232 87 180 2.22e-15 75.5
MS.gene061342.t1 AT5G03790 43.820 89 50 0 143 231 77 165 3.09e-15 73.9
MS.gene061342.t1 AT2G18550 43.137 102 53 1 137 238 55 151 7.98e-15 72.8
MS.gene061342.t1 AT4G40060 43.299 97 48 1 141 230 57 153 1.60e-14 72.8
MS.gene061342.t1 AT1G27045 45.833 96 45 1 143 231 68 163 2.89e-14 71.6
MS.gene061342.t1 AT1G27045 56.061 66 29 0 143 208 68 133 4.41e-14 70.5
MS.gene061342.t1 AT2G22430 45.833 96 48 1 141 232 60 155 4.64e-14 71.6
MS.gene061342.t1 AT1G27045 56.061 66 29 0 143 208 25 90 4.95e-14 69.7
MS.gene061342.t1 AT1G27045 56.061 66 29 0 143 208 25 90 4.95e-14 69.7
MS.gene061342.t1 AT1G27045 56.061 66 29 0 143 208 17 82 5.83e-14 69.3
MS.gene061342.t1 AT2G36610 36.937 111 58 2 133 231 57 167 2.19e-13 67.8
MS.gene061342.t1 AT2G46680 42.105 95 51 1 146 236 35 129 5.95e-13 67.8
MS.gene061342.t1 AT2G46680 42.045 88 51 0 146 233 35 122 6.28e-13 67.8
MS.gene061342.t1 AT4G36740 40.385 104 55 2 135 238 50 146 6.63e-13 67.0
MS.gene061342.t1 AT4G36740 40.385 104 55 2 135 238 49 145 6.86e-13 67.0
MS.gene061342.t1 AT5G03790 41.758 91 53 0 143 233 77 167 2.28e-12 65.1
MS.gene061342.t1 AT5G06710 45.000 100 41 4 88 180 135 227 2.38e-11 63.2
MS.gene061342.t1 AT5G06710 45.000 100 41 4 88 180 135 227 2.38e-11 63.2
MS.gene061342.t1 AT3G61890 40.964 83 49 0 146 228 33 115 5.52e-11 62.0
MS.gene061342.t1 AT1G79840 38.947 95 55 1 132 223 90 184 9.98e-11 62.8

Find 90 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCATGGTCGGGAACGATTT+TGG 0.169525 8.2:+83320685 None:intergenic
GAAAGATGATGAATTTAGTT+TGG 0.275241 8.2:-83323014 MS.gene061342:CDS
GAAAACACATACCATGAAAA+TGG 0.282242 8.2:+83322864 None:intergenic
CACTTCAGTTTGCTTCAACT+TGG 0.366945 8.2:+83320992 None:intergenic
TTAAGCTTGAGTTTAGGATT+TGG 0.367633 8.2:-83322989 MS.gene061342:CDS
AAAGATGATGAATTTAGTTT+GGG 0.373079 8.2:-83323013 MS.gene061342:CDS
CTTTGGTTTGTAACCGTTTG+AGG 0.393528 8.2:+83322908 None:intergenic
TCTGCTTCAGCCAGCCCGTC+AGG 0.405875 8.2:-83320759 MS.gene061342:CDS
AAAAGGAAGTGCAAGAGCTT+AGG 0.411234 8.2:-83320911 MS.gene061342:CDS
TTGGGTTTAAGCTTGAGTTT+AGG 0.412484 8.2:-83322995 MS.gene061342:CDS
GGCCAACACCTGTCAACCCT+TGG 0.414534 8.2:-83320722 MS.gene061342:CDS
CAACACTCCACTACCATTCA+TGG 0.418174 8.2:-83322300 MS.gene061342:CDS
AACACTTGGTCCTGACGGGC+TGG 0.423515 8.2:+83320749 None:intergenic
GAGTCACCTTCAAAGGAGTT+TGG 0.430355 8.2:+83322945 None:intergenic
AACCACACCTCCACTTGTCT+TGG 0.434860 8.2:+83321688 None:intergenic
AAAGGAAGTGCAAGAGCTTA+GGG 0.439484 8.2:-83320910 MS.gene061342:CDS
AGTTATTGAAGGAAACCCTT+TGG 0.441554 8.2:+83322891 None:intergenic
CAACAACCAAACTCCTTTGA+AGG 0.444372 8.2:-83322951 MS.gene061342:CDS
GTTGTTGGAAGAAACTTTCA+AGG 0.454317 8.2:-83322054 MS.gene061342:CDS
ATGCTTGACACGGTGCTGTT+CGG 0.456039 8.2:+83322242 None:intergenic
GCCCAAGGGTTGACAGGTGT+TGG 0.475640 8.2:+83320720 None:intergenic
CGTTGAACACTTGGTCCTGA+CGG 0.477048 8.2:+83320744 None:intergenic
GGTGTTGGCCGTTGAACACT+TGG 0.478877 8.2:+83320735 None:intergenic
GCCAACACCTGTCAACCCTT+GGG 0.479663 8.2:-83320721 MS.gene061342:CDS
GATGATGACGGCGGAGGTTG+CGG 0.480696 8.2:-83322131 MS.gene061342:CDS
AGACTCTAACTGATGAGAAT+AGG 0.482124 8.2:-83320941 MS.gene061342:CDS
GGCGGAGGTTGCGGTGGCGA+CGG 0.488707 8.2:-83322122 MS.gene061342:CDS
AGCAGCAGCAGCTGCTGATG+AGG 0.490620 8.2:+83320788 None:intergenic
GTTGAACACTTGGTCCTGAC+GGG 0.490686 8.2:+83320745 None:intergenic
TCGGCCGTCGCCGCCATGAA+TGG 0.494934 8.2:+83322287 None:intergenic
GGTTCATGTTCTCGCGGCGG+CGG 0.496150 8.2:-83322161 MS.gene061342:CDS
GAAGGAACAGTCAATGTTGT+TGG 0.498061 8.2:-83322069 MS.gene061342:CDS
ATTGCCGCCCAAGGGTTGAC+AGG 0.503094 8.2:+83320714 None:intergenic
TTCATTTGCATGTAGAGTTG+TGG 0.503551 8.2:+83320873 None:intergenic
CGGCGACACGCTCGCACGAA+GGG 0.511247 8.2:+83320826 None:intergenic
TCATTTCTCCGCGGAATAGA+CGG 0.512734 8.2:-83322323 MS.gene061342:CDS
TGAAAACGACGCCGTCGAAA+GGG 0.514530 8.2:-83322183 MS.gene061342:CDS
TGGATAGACATTGCCGCCCA+AGG 0.515878 8.2:+83320705 None:intergenic
GTCGCCGCCATGAATGGTAG+TGG 0.516822 8.2:+83322293 None:intergenic
CTTTCCACTGATGCTTGACA+CGG 0.517994 8.2:+83322232 None:intergenic
AAAATGGAACAAGTTATTGA+AGG 0.520682 8.2:+83322880 None:intergenic
AGAAGCGAAAGAGAAGGAAA+CGG 0.525139 8.2:-83322209 MS.gene061342:CDS
CATGCACAAGCCTCCTCAAA+CGG 0.530251 8.2:-83322921 MS.gene061342:CDS
GGAGCATAATACTCTGAATC+CGG 0.531921 8.2:-83322033 MS.gene061342:intron
GCGACGGCCGACGATGAAAA+CGG 0.538257 8.2:-83322275 MS.gene061342:CDS
TGCATGTGAGTCACCTTCAA+AGG 0.543980 8.2:+83322938 None:intergenic
TGACGGGCTGGCTGAAGCAG+AGG 0.544924 8.2:+83320761 None:intergenic
TAGAGCGAATACTCATGGTC+GGG 0.553208 8.2:+83320674 None:intergenic
CTCTAACTGATGAGAATAGG+AGG 0.555223 8.2:-83320938 MS.gene061342:CDS
TCAGAAACAAAAGCAAGCAT+TGG 0.557840 8.2:-83321731 MS.gene061342:intron
GGAAAGAGAAGCGAAAGAGA+AGG 0.564040 8.2:-83322215 MS.gene061342:CDS
TGAGAATAGGAGGTTGCAAA+AGG 0.565911 8.2:-83320928 MS.gene061342:CDS
CTCAAACGGTTACAAACCAA+AGG 0.569424 8.2:-83322907 MS.gene061342:CDS
ATGAAAACGACGCCGTCGAA+AGG 0.571630 8.2:-83322184 MS.gene061342:CDS
GGATAGACATTGCCGCCCAA+GGG 0.571951 8.2:+83320706 None:intergenic
GACGGGCTGGCTGAAGCAGA+GGG 0.572019 8.2:+83320762 None:intergenic
GGGCACATTGTAAGAGTGGT+GGG 0.573198 8.2:+83320846 None:intergenic
CAAGTTGAAGCAAACTGAAG+TGG 0.573772 8.2:-83320991 MS.gene061342:CDS
CTAGAGCGAATACTCATGGT+CGG 0.575049 8.2:+83320673 None:intergenic
CGAAGGGCACATTGTAAGAG+TGG 0.588926 8.2:+83320842 None:intergenic
AACACCTGTCAACCCTTGGG+CGG 0.591322 8.2:-83320718 MS.gene061342:CDS
TCAAACGGTTACAAACCAAA+GGG 0.592760 8.2:-83322906 MS.gene061342:CDS
ACATTGTAAGAGTGGTGGGA+GGG 0.595529 8.2:+83320850 None:intergenic
ACGGCGACACGCTCGCACGA+AGG 0.599222 8.2:+83320825 None:intergenic
AGCCAAGACAAGTGGAGGTG+TGG 0.599876 8.2:-83321690 MS.gene061342:CDS
TGGTTTGTAACCGTTTGAGG+AGG 0.599884 8.2:+83322911 None:intergenic
AGCACCGTGTCAAGCATCAG+TGG 0.602338 8.2:-83322236 MS.gene061342:CDS
AGGGCACATTGTAAGAGTGG+TGG 0.603305 8.2:+83320845 None:intergenic
ACTACCATTCATGGCGGCGA+CGG 0.605001 8.2:-83322291 MS.gene061342:CDS
CGTCAGGACCAAGTGTTCAA+CGG 0.606766 8.2:-83320743 MS.gene061342:CDS
AGAACATGAACCCCTTTCGA+CGG 0.611067 8.2:+83322172 None:intergenic
CACATTGTAAGAGTGGTGGG+AGG 0.613092 8.2:+83320849 None:intergenic
GAAAACGACGCCGTCGAAAG+GGG 0.619048 8.2:-83322182 MS.gene061342:CDS
GTGGCGACGGTGATTCGTCA+AGG 0.623006 8.2:-83322109 MS.gene061342:CDS
GAATCTGAAGCCAAGACAAG+TGG 0.625066 8.2:-83321698 MS.gene061342:CDS
GATGACGGCGGAGGTTGCGG+TGG 0.629392 8.2:-83322128 MS.gene061342:CDS
GAAGAAGCTGAGGTTGTCGA+AGG 0.629748 8.2:-83322087 MS.gene061342:CDS
AGTGATGATGATGACGGCGG+AGG 0.634764 8.2:-83322137 MS.gene061342:CDS
TCTGAAGCCAAGACAAGTGG+AGG 0.635230 8.2:-83321695 MS.gene061342:CDS
ATTCGTCAAGGAAGAAGCTG+AGG 0.662832 8.2:-83322097 MS.gene061342:CDS
GAAAGGGGTTCATGTTCTCG+CGG 0.668678 8.2:-83322167 MS.gene061342:CDS
GGCGGAAGTGATGATGATGA+CGG 0.668902 8.2:-83322143 MS.gene061342:CDS
GGTTTCAAAACAGAAGAGCA+AGG 0.676969 8.2:-83321669 MS.gene061342:intron
CAGATCTAAGCACAGAAACG+AGG 0.678976 8.2:-83322346 MS.gene061342:CDS
GAGTGTTGCCGTCTATTCCG+CGG 0.683806 8.2:+83322315 None:intergenic
TGAGGACGATGCAGATGACA+CGG 0.688825 8.2:+83320806 None:intergenic
AGGGGTTCATGTTCTCGCGG+CGG 0.699189 8.2:-83322164 MS.gene061342:CDS
GGAAGTGATGATGATGACGG+CGG 0.716365 8.2:-83322140 MS.gene061342:CDS
CACTCCACTACCATTCATGG+CGG 0.720863 8.2:-83322297 MS.gene061342:CDS
GAAACGAGGTCATTTCTCCG+CGG 0.771153 8.2:-83322332 MS.gene061342:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTATAAAAAAAAAA+TGG + chr8.2:83321124-83321143 None:intergenic 0.0%
!! AAAAAAAAAAAAAACAACCT+CGG + chr8.2:83321851-83321870 None:intergenic 15.0%
!! AATCAGAAAGTAAATTAAAA+TGG + chr8.2:83321236-83321255 None:intergenic 15.0%
!! ATCAGAAAGTAAATTAAAAT+GGG + chr8.2:83321235-83321254 None:intergenic 15.0%
!! GAAAAAAAAAATTTGAAGAA+AGG + chr8.2:83320892-83320911 None:intergenic 15.0%
!! GTAATTATCAACTAAATTTA+AGG + chr8.2:83321304-83321323 None:intergenic 15.0%
!!! AGGTTGTTTTTTTTTTTTTT+TGG - chr8.2:83321851-83321870 MS.gene061342:intron 15.0%
!!! ATTTCTCTTCATATTTTTTA+GGG - chr8.2:83320984-83321003 MS.gene061342:CDS 15.0%
!!! CATTTTTGGTTTTTTTTTTT+TGG - chr8.2:83321029-83321048 MS.gene061342:intron 15.0%
!!! GTCATTTTTGAATATTAATT+TGG - chr8.2:83322091-83322110 MS.gene061342:CDS 15.0%
!!! TTATTATAATCTTTTGATGA+TGG - chr8.2:83322459-83322478 MS.gene061342:intron 15.0%
!!! TTGTTTTTATTATTGTTTTG+AGG - chr8.2:83321820-83321839 MS.gene061342:intron 15.0%
!!! TTTTTCATTATATGATTCAA+GGG + chr8.2:83321097-83321116 None:intergenic 15.0%
!! AAAAAACCAAAAATGAGAAA+TGG + chr8.2:83321024-83321043 None:intergenic 20.0%
!! AAAAACCAAAAATGAGAAAT+GGG + chr8.2:83321023-83321042 None:intergenic 20.0%
!! GGAATTAAAAATGAAAAGAA+TGG + chr8.2:83320914-83320933 None:intergenic 20.0%
!! TGTAAACTCTTAAAATTGAA+AGG + chr8.2:83322507-83322526 None:intergenic 20.0%
!!! AAAGATGATGAATTTAGTTT+GGG - chr8.2:83320695-83320714 MS.gene061342:CDS 20.0%
!!! GATTTCTCTTCATATTTTTT+AGG - chr8.2:83320983-83321002 MS.gene061342:CDS 20.0%
!!! GTAATTCAGTTTTATGTTAA+GGG - chr8.2:83321722-83321741 MS.gene061342:CDS 20.0%
!!! GTTTTTCATTATATGATTCA+AGG + chr8.2:83321098-83321117 None:intergenic 20.0%
!!! GTTTTTTTTTTTTGGTTGAA+TGG - chr8.2:83321037-83321056 MS.gene061342:intron 20.0%
!!! TATATTTGGAAGTGTAAATT+TGG - chr8.2:83321777-83321796 MS.gene061342:intron 20.0%
!!! TGTAATTCAGTTTTATGTTA+AGG - chr8.2:83321721-83321740 MS.gene061342:CDS 20.0%
!!! TTTTTTTGGTTGAGTAATTA+AGG - chr8.2:83321865-83321884 MS.gene061342:intron 20.0%
! AAAACCAAAAATGAGAAATG+GGG + chr8.2:83321022-83321041 None:intergenic 25.0%
! AAAATGGAACAAGTTATTGA+AGG + chr8.2:83320831-83320850 None:intergenic 25.0%
! AAAATTAACAATGCATCAAG+AGG + chr8.2:83320935-83320954 None:intergenic 25.0%
! ACTGTTTGGATCATATTAAT+TGG - chr8.2:83322212-83322231 MS.gene061342:CDS 25.0%
! ATATGAAGAGAAATCAAGAA+TGG + chr8.2:83320978-83320997 None:intergenic 25.0%
! ATTCATCTCATCAAAGAAAA+TGG + chr8.2:83321191-83321210 None:intergenic 25.0%
! GAAAGATGATGAATTTAGTT+TGG - chr8.2:83320694-83320713 MS.gene061342:CDS 25.0%
! GAAAGGAAACATAGAAATTT+AGG + chr8.2:83320875-83320894 None:intergenic 25.0%
! GAATTTCATTACAGGATAAA+AGG - chr8.2:83321753-83321772 MS.gene061342:intron 25.0%
! TATGAAGAGAAATCAAGAAT+GGG + chr8.2:83320977-83320996 None:intergenic 25.0%
! TTATGAGGGAGTAAAAAATT+AGG - chr8.2:83322341-83322360 MS.gene061342:CDS 25.0%
! TTCATCTCATCAAAGAAAAT+GGG + chr8.2:83321190-83321209 None:intergenic 25.0%
!! AACACAACTTAACTTTTATG+AGG - chr8.2:83322326-83322345 MS.gene061342:CDS 25.0%
!! ACACAACTTAACTTTTATGA+GGG - chr8.2:83322327-83322346 MS.gene061342:CDS 25.0%
!! CTTATTTGGAATTTCATTAC+AGG - chr8.2:83321745-83321764 MS.gene061342:intron 25.0%
!! TTTTATGTTAAGGGCTTATT+TGG - chr8.2:83321731-83321750 MS.gene061342:intron 25.0%
!!! AATAACTTGTTCCATTTTCA+TGG - chr8.2:83320833-83320852 MS.gene061342:CDS 25.0%
!!! ATAATCTTTTGATGATGGAT+TGG - chr8.2:83322464-83322483 MS.gene061342:intron 25.0%
!!! TAATCTTTTGATGATGGATT+GGG - chr8.2:83322465-83322484 MS.gene061342:intron 25.0%
!!! TTTTTTTGGTTGAATGGTAA+GGG - chr8.2:83321043-83321062 MS.gene061342:intron 25.0%
!!! TTTTTTTTGGTTGAATGGTA+AGG - chr8.2:83321042-83321061 MS.gene061342:intron 25.0%
ACAGGATAAAAGGCTATATT+TGG - chr8.2:83321763-83321782 MS.gene061342:intron 30.0%
GAAAACACATACCATGAAAA+TGG + chr8.2:83320847-83320866 None:intergenic 30.0%
GTTGTATTGTAACAAGTATG+TGG - chr8.2:83322381-83322400 MS.gene061342:intron 30.0%
TAAACATTGTTGTGATGTTG+TGG - chr8.2:83322125-83322144 MS.gene061342:CDS 30.0%
TAATATGATCCAAACAGTGT+GGG + chr8.2:83322210-83322229 None:intergenic 30.0%
TTAATATGATCCAAACAGTG+TGG + chr8.2:83322211-83322230 None:intergenic 30.0%
! TACATTTTTGTGCATGTAGT+AGG + chr8.2:83322430-83322449 None:intergenic 30.0%
! TTAAGCTTGAGTTTAGGATT+TGG - chr8.2:83320719-83320738 MS.gene061342:CDS 30.0%
!!! TTTTTTGGTTGAATGGTAAG+GGG - chr8.2:83321044-83321063 MS.gene061342:intron 30.0%
AAAATGTGACATGAGTGCTT+TGG - chr8.2:83322678-83322697 MS.gene061342:intron 35.0%
AATATGATCCAAACAGTGTG+GGG + chr8.2:83322209-83322228 None:intergenic 35.0%
AGACTCTAACTGATGAGAAT+AGG - chr8.2:83322767-83322786 MS.gene061342:intron 35.0%
AGTTATTGAAGGAAACCCTT+TGG + chr8.2:83320820-83320839 None:intergenic 35.0%
CACAACAAATCCACATACAT+TGG + chr8.2:83322599-83322618 None:intergenic 35.0%
CATGTACAAACCAATGTATG+TGG - chr8.2:83322586-83322605 MS.gene061342:intron 35.0%
GGTTATACCAACGATAAGAA+GGG + chr8.2:83321076-83321095 None:intergenic 35.0%
GTTATACCAACGATAAGAAG+GGG + chr8.2:83321075-83321094 None:intergenic 35.0%
GTTGTTGGAAGAAACTTTCA+AGG - chr8.2:83321654-83321673 MS.gene061342:intron 35.0%
TATTGTGTTCCATGCTGTTA+TGG - chr8.2:83322646-83322665 MS.gene061342:intron 35.0%
TCAAACGGTTACAAACCAAA+GGG - chr8.2:83320802-83320821 MS.gene061342:CDS 35.0%
TCAGAAACAAAAGCAAGCAT+TGG - chr8.2:83321977-83321996 MS.gene061342:intron 35.0%
TTCATTTGCATGTAGAGTTG+TGG + chr8.2:83322838-83322857 None:intergenic 35.0%
TTGGGTTTAAGCTTGAGTTT+AGG - chr8.2:83320713-83320732 MS.gene061342:CDS 35.0%
! AGAACCCCATTTCTCATTTT+TGG - chr8.2:83321015-83321034 MS.gene061342:intron 35.0%
! ATTTTTGTGCATGTAGTAGG+TGG + chr8.2:83322427-83322446 None:intergenic 35.0%
! TTTTTCCTTCCATAACAGCA+TGG + chr8.2:83322658-83322677 None:intergenic 35.0%
! TTTTTGTGCATGTAGTAGGT+GGG + chr8.2:83322426-83322445 None:intergenic 35.0%
!! TTTGCATGTGAGTGTTTTAG+TGG + chr8.2:83321334-83321353 None:intergenic 35.0%
!!! ATTGTTTTGAGGTTATTCCG+AGG - chr8.2:83321831-83321850 MS.gene061342:intron 35.0%
ACAGTGTGGGGTATATAGTA+TGG + chr8.2:83322197-83322216 None:intergenic 40.0%
ACTATATACCCCACACTGTT+TGG - chr8.2:83322198-83322217 MS.gene061342:CDS 40.0%
AGAAGCGAAAGAGAAGGAAA+CGG - chr8.2:83321499-83321518 MS.gene061342:intron 40.0%
CAACAACCAAACTCCTTTGA+AGG - chr8.2:83320757-83320776 MS.gene061342:CDS 40.0%
CTCAAACGGTTACAAACCAA+AGG - chr8.2:83320801-83320820 MS.gene061342:CDS 40.0%
CTCTAACTGATGAGAATAGG+AGG - chr8.2:83322770-83322789 MS.gene061342:intron 40.0%
GAAGGAACAGTCAATGTTGT+TGG - chr8.2:83321639-83321658 MS.gene061342:intron 40.0%
GGAGCATAATACTCTGAATC+CGG - chr8.2:83321675-83321694 MS.gene061342:CDS 40.0%
GGGTTATACCAACGATAAGA+AGG + chr8.2:83321077-83321096 None:intergenic 40.0%
GGTTTCAAAACAGAAGAGCA+AGG - chr8.2:83322039-83322058 MS.gene061342:CDS 40.0%
GTGGCAACATGAGTATGTTA+GGG - chr8.2:83322400-83322419 MS.gene061342:intron 40.0%
TACCACTACAACAAACTCAC+CGG + chr8.2:83321697-83321716 None:intergenic 40.0%
TGAGAATAGGAGGTTGCAAA+AGG - chr8.2:83322780-83322799 MS.gene061342:intron 40.0%
TGTGGCAACATGAGTATGTT+AGG - chr8.2:83322399-83322418 MS.gene061342:intron 40.0%
! AAAAGGAAGTGCAAGAGCTT+AGG - chr8.2:83322797-83322816 MS.gene061342:intron 40.0%
! AAAGGAAGTGCAAGAGCTTA+GGG - chr8.2:83322798-83322817 MS.gene061342:intron 40.0%
! CAAGTTGAAGCAAACTGAAG+TGG - chr8.2:83322717-83322736 MS.gene061342:intron 40.0%
! CACTTCAGTTTGCTTCAACT+TGG + chr8.2:83322719-83322738 None:intergenic 40.0%
! TGAAACTCCGTTTTCATCGT+CGG + chr8.2:83321443-83321462 None:intergenic 40.0%
!! CTTTGGTTTGTAACCGTTTG+AGG + chr8.2:83320803-83320822 None:intergenic 40.0%
!!! CTTTTGATGATGGATTGGGA+AGG - chr8.2:83322469-83322488 MS.gene061342:intron 40.0%
AGAACATGAACCCCTTTCGA+CGG + chr8.2:83321539-83321558 None:intergenic 45.0%
AGGTGAGCTTTCTTCTTCTC+AGG - chr8.2:83322059-83322078 MS.gene061342:CDS 45.0%
ATTCGTCAAGGAAGAAGCTG+AGG - chr8.2:83321611-83321630 MS.gene061342:intron 45.0%
CAACACTCCACTACCATTCA+TGG - chr8.2:83321408-83321427 MS.gene061342:intron 45.0%
CAGATCTAAGCACAGAAACG+AGG - chr8.2:83321362-83321381 MS.gene061342:intron 45.0%
CTTTCCACTGATGCTTGACA+CGG + chr8.2:83321479-83321498 None:intergenic 45.0%
GAAAAGCCCCTTCTTATCGT+TGG - chr8.2:83321066-83321085 MS.gene061342:intron 45.0%
GAGTCACCTTCAAAGGAGTT+TGG + chr8.2:83320766-83320785 None:intergenic 45.0%
GGAAAGAGAAGCGAAAGAGA+AGG - chr8.2:83321493-83321512 MS.gene061342:intron 45.0%
GGTGAGCTTTCTTCTTCTCA+GGG - chr8.2:83322060-83322079 MS.gene061342:CDS 45.0%
GTGAGCTTTCTTCTTCTCAG+GGG - chr8.2:83322061-83322080 MS.gene061342:CDS 45.0%
GTGTTCCATGCTGTTATGGA+AGG - chr8.2:83322650-83322669 MS.gene061342:intron 45.0%
TCATTTCTCCGCGGAATAGA+CGG - chr8.2:83321385-83321404 MS.gene061342:intron 45.0%
TGCATGTGAGTCACCTTCAA+AGG + chr8.2:83320773-83320792 None:intergenic 45.0%
! ACATTGTAAGAGTGGTGGGA+GGG + chr8.2:83322861-83322880 None:intergenic 45.0%
! GAATCTGAAGCCAAGACAAG+TGG - chr8.2:83322010-83322029 MS.gene061342:intron 45.0%
! GTGCTTTGGTTCAATGATGC+AGG - chr8.2:83322692-83322711 MS.gene061342:intron 45.0%
! TGGTTTGTAACCGTTTGAGG+AGG + chr8.2:83320800-83320819 None:intergenic 45.0%
!! ATCCGGTGAGTTTGTTGTAG+TGG - chr8.2:83321692-83321711 MS.gene061342:CDS 45.0%
!! TATTTATTTATGAATTAAAA+CGG - chr8.2:83321259-83321278 MS.gene061342:intron 5.0%
AACCACACCTCCACTTGTCT+TGG + chr8.2:83322023-83322042 None:intergenic 50.0%
AGGGCACATTGTAAGAGTGG+TGG + chr8.2:83322866-83322885 None:intergenic 50.0%
ATGAAAACGACGCCGTCGAA+AGG - chr8.2:83321524-83321543 MS.gene061342:intron 50.0%
CACTCCACTACCATTCATGG+CGG - chr8.2:83321411-83321430 MS.gene061342:intron 50.0%
CATGCACAAGCCTCCTCAAA+CGG - chr8.2:83320787-83320806 MS.gene061342:CDS 50.0%
CGTCAGGACCAAGTGTTCAA+CGG - chr8.2:83322965-83322984 MS.gene061342:CDS 50.0%
CGTTGAACACTTGGTCCTGA+CGG + chr8.2:83322967-83322986 None:intergenic 50.0%
GAAACGAGGTCATTTCTCCG+CGG - chr8.2:83321376-83321395 MS.gene061342:intron 50.0%
GAAAGGGGTTCATGTTCTCG+CGG - chr8.2:83321541-83321560 MS.gene061342:intron 50.0%
GAAGAAGCTGAGGTTGTCGA+AGG - chr8.2:83321621-83321640 MS.gene061342:intron 50.0%
GGGCACATTGTAAGAGTGGT+GGG + chr8.2:83322865-83322884 None:intergenic 50.0%
GTTGAACACTTGGTCCTGAC+GGG + chr8.2:83322966-83322985 None:intergenic 50.0%
TGAAAACGACGCCGTCGAAA+GGG - chr8.2:83321525-83321544 MS.gene061342:intron 50.0%
TGAGGACGATGCAGATGACA+CGG + chr8.2:83322905-83322924 None:intergenic 50.0%
! CACATTGTAAGAGTGGTGGG+AGG + chr8.2:83322862-83322881 None:intergenic 50.0%
! CGAAGGGCACATTGTAAGAG+TGG + chr8.2:83322869-83322888 None:intergenic 50.0%
! GGCGGAAGTGATGATGATGA+CGG - chr8.2:83321565-83321584 MS.gene061342:intron 50.0%
! TCTGAAGCCAAGACAAGTGG+AGG - chr8.2:83322013-83322032 MS.gene061342:intron 50.0%
!! ATGCTTGACACGGTGCTGTT+CGG + chr8.2:83321469-83321488 None:intergenic 50.0%
!! GGAAGTGATGATGATGACGG+CGG - chr8.2:83321568-83321587 MS.gene061342:intron 50.0%
AACACCTGTCAACCCTTGGG+CGG - chr8.2:83322990-83323009 MS.gene061342:CDS 55.0%
ACTACCATTCATGGCGGCGA+CGG - chr8.2:83321417-83321436 MS.gene061342:intron 55.0%
AGCACCGTGTCAAGCATCAG+TGG - chr8.2:83321472-83321491 MS.gene061342:intron 55.0%
AGCCAAGACAAGTGGAGGTG+TGG - chr8.2:83322018-83322037 MS.gene061342:intron 55.0%
GAAAACGACGCCGTCGAAAG+GGG - chr8.2:83321526-83321545 MS.gene061342:intron 55.0%
GCCAACACCTGTCAACCCTT+GGG - chr8.2:83322987-83323006 MS.gene061342:CDS 55.0%
GGATAGACATTGCCGCCCAA+GGG + chr8.2:83323005-83323024 None:intergenic 55.0%
TGGATAGACATTGCCGCCCA+AGG + chr8.2:83323006-83323025 None:intergenic 55.0%
! GAGTGTTGCCGTCTATTCCG+CGG + chr8.2:83321396-83321415 None:intergenic 55.0%
!! AGTGATGATGATGACGGCGG+AGG - chr8.2:83321571-83321590 MS.gene061342:intron 55.0%
!! GGTGTTGGCCGTTGAACACT+TGG + chr8.2:83322976-83322995 None:intergenic 55.0%
AGCAGCAGCAGCTGCTGATG+AGG + chr8.2:83322923-83322942 None:intergenic 60.0%
AGGGGTTCATGTTCTCGCGG+CGG - chr8.2:83321544-83321563 MS.gene061342:intron 60.0%
ATTGCCGCCCAAGGGTTGAC+AGG + chr8.2:83322997-83323016 None:intergenic 60.0%
GCCCAAGGGTTGACAGGTGT+TGG + chr8.2:83322991-83323010 None:intergenic 60.0%
GCGACGGCCGACGATGAAAA+CGG - chr8.2:83321433-83321452 MS.gene061342:intron 60.0%
GGCCAACACCTGTCAACCCT+TGG - chr8.2:83322986-83323005 MS.gene061342:CDS 60.0%
GTCGCCGCCATGAATGGTAG+TGG + chr8.2:83321418-83321437 None:intergenic 60.0%
GTGGCGACGGTGATTCGTCA+AGG - chr8.2:83321599-83321618 MS.gene061342:intron 60.0%
! AACACTTGGTCCTGACGGGC+TGG + chr8.2:83322962-83322981 None:intergenic 60.0%
!! GATGATGACGGCGGAGGTTG+CGG - chr8.2:83321577-83321596 MS.gene061342:intron 60.0%
GGTTCATGTTCTCGCGGCGG+CGG - chr8.2:83321547-83321566 MS.gene061342:intron 65.0%
TCTGCTTCAGCCAGCCCGTC+AGG - chr8.2:83322949-83322968 MS.gene061342:CDS 65.0%
!! GACGGGCTGGCTGAAGCAGA+GGG + chr8.2:83322949-83322968 None:intergenic 65.0%
!! TGACGGGCTGGCTGAAGCAG+AGG + chr8.2:83322950-83322969 None:intergenic 65.0%
ACGGCGACACGCTCGCACGA+AGG + chr8.2:83322886-83322905 None:intergenic 70.0%
CGGCGACACGCTCGCACGAA+GGG + chr8.2:83322885-83322904 None:intergenic 70.0%
TCGGCCGTCGCCGCCATGAA+TGG + chr8.2:83321424-83321443 None:intergenic 70.0%
! GATGACGGCGGAGGTTGCGG+TGG - chr8.2:83321580-83321599 MS.gene061342:intron 70.0%
GGCGGAGGTTGCGGTGGCGA+CGG - chr8.2:83321586-83321605 MS.gene061342:intron 75.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 83320686 83323044 83320686 ID=MS.gene061342
chr8.2 mRNA 83320686 83323044 83320686 ID=MS.gene061342.t1;Parent=MS.gene061342
chr8.2 exon 83322876 83323044 83322876 ID=MS.gene061342.t1.exon1;Parent=MS.gene061342.t1
chr8.2 CDS 83322876 83323044 83322876 ID=cds.MS.gene061342.t1;Parent=MS.gene061342.t1
chr8.2 exon 83322034 83322365 83322034 ID=MS.gene061342.t1.exon2;Parent=MS.gene061342.t1
chr8.2 CDS 83322034 83322365 83322034 ID=cds.MS.gene061342.t1;Parent=MS.gene061342.t1
chr8.2 exon 83321670 83321749 83321670 ID=MS.gene061342.t1.exon3;Parent=MS.gene061342.t1
chr8.2 CDS 83321670 83321749 83321670 ID=cds.MS.gene061342.t1;Parent=MS.gene061342.t1
chr8.2 exon 83320686 83321016 83320686 ID=MS.gene061342.t1.exon4;Parent=MS.gene061342.t1
chr8.2 CDS 83320686 83321016 83320686 ID=cds.MS.gene061342.t1;Parent=MS.gene061342.t1
Gene Sequence

>MS.gene061342

ATGGGTGAGAAAGATGATGAATTTAGTTTGGGTTTAAGCTTGAGTTTAGGATTTGGAGAATATGCAAATAACAACAACCAAACTCCTTTGAAGGTGACTCACATGCACAAGCCTCCTCAAACGGTTACAAACCAAAGGGTTTCCTTCAATAACTTGTTCCATTTTCATGGTATGTGTTTTCTCTCTCCTAAATTTCTATGTTTCCTTTCTTCAAATTTTTTTTTTCCATTCTTTTCATTTTTAATTCCTCTTGATGCATTGTTAATTTTTTTTTTTGTTTTGAAAAAACCCATTCTTGATTTCTCTTCATATTTTTTAGGGTAAATACTAGAACCCCATTTCTCATTTTTGGTTTTTTTTTTTTGGTTGAATGGTAAGGGGAAAAGCCCCTTCTTATCGTTGGTATAACCCTTGAATCATATAATGAAAAACATACCATTTTTTTTTTATAAAAAAAACTATTTTTTACACTAAAAATAGATTTTTTAACATAACTAAAAACCCATTTTCTTTGATGAGATGAATTCAAGTCATGAAAATGAAGAACCCATTTTAATTTACTTTCTGATTTTTTATTTATTTATGAATTAAAACGGTTTTGTTACAAAAAAATAGCCTTAAATTTAGTTGATAATTACACGTTTACCACTAAAACACTCACATGCAAATCACGTGACAGATCTAAGCACAGAAACGAGGTCATTTCTCCGCGGAATAGACGGCAACACTCCACTACCATTCATGGCGGCGACGGCCGACGATGAAAACGGAGTTTCATCACCGAACAGCACCGTGTCAAGCATCAGTGGAAAGAGAAGCGAAAGAGAAGGAAACGGAGATGAAAACGACGCCGTCGAAAGGGGTTCATGTTCTCGCGGCGGCGGAAGTGATGATGATGACGGCGGAGGTTGCGGTGGCGACGGTGATTCGTCAAGGAAGAAGCTGAGGTTGTCGAAGGAACAGTCAATGTTGTTGGAAGAAACTTTCAAGGAGCATAATACTCTGAATCCGGTGAGTTTGTTGTAGTGGTATTTCTGTAATTCAGTTTTATGTTAAGGGCTTATTTGGAATTTCATTACAGGATAAAAGGCTATATTTGGAAGTGTAAATTTGGTTGTTTTGACTTGTTCTTTTTTGTTTTTATTATTGTTTTGAGGTTATTCCGAGGTTGTTTTTTTTTTTTTTTGGTTGAGTAATTAAGGTTGTCTTTCTTTATATAATCTTAAAATAATTATTTTAATTATTACTTTGATTTTGATTTTAATTATTATTATTATTATTGTTATTATTATCAGAAACAAAAGCAAGCATTGGCAAAGCAGTTGAATCTGAAGCCAAGACAAGTGGAGGTGTGGTTTCAAAACAGAAGAGCAAGGTGAGCTTTCTTCTTCTCAGGGGCATTACTGTCATTTTTGAATATTAATTTGGAATTAGTAAAATAAACATTGTTGTGATGTTGTGGCATTGTTTGTTGTGTCATGTGTGACTTTGTAAGCTTCTGTTGCATACCATACTATATACCCCACACTGTTTGGATCATATTAATTGGTTTTGTTTTTTACAAATTAAAATGTTAGCTATCACTAAAAAAAATTAAAATGTTAATATTACTCTTTGAAATAAATCTATGAGTGATCAATAACACAACTTAACTTTTATGAGGGAGTAAAAAATTAGGCATAAAAAAAATTTGAAGTTGTATTGTAACAAGTATGTGGCAACATGAGTATGTTAGGGCCCACCTACTACATGCACAAAAATGTAACAAGCATGTTATTATAATCTTTTGATGATGGATTGGGAAGGCAAAATTTGAAACCTTTCAATTTTAAGAGTTTACATACATTCATTAATTTCTCATGAGTTAAATTAGTTAATAGAAGAAATACTTTTAACATGTCATGTACAAACCAATGTATGTGGATTTGTTGTGTTATGTGTATGTGTTTCATGTGTGTTGTATTGTGTTCCATGCTGTTATGGAAGGAAAAAAAAATGTGACATGAGTGCTTTGGTTCAATGATGCAGGACCAAGTTGAAGCAAACTGAAGTGGATTGTGAGTACTTGAAGAGATGCTGTGAGACTCTAACTGATGAGAATAGGAGGTTGCAAAAGGAAGTGCAAGAGCTTAGGGCATTGAAGTTATCTCCACAACTCTACATGCAAATGAACCCTCCCACCACTCTTACAATGTGCCCTTCGTGCGAGCGTGTCGCCGTGTCATCTGCATCGTCCTCATCAGCAGCTGCTGCTGCTCTTCCCTCTGCTTCAGCCAGCCCGTCAGGACCAAGTGTTCAACGGCCAACACCTGTCAACCCTTGGGCGGCAATGTCTATCCAAAATCGTTCCCGACCATGA
Protein sequence

>MS.gene061342.t1

MGEKDDEFSLGLSLSLGFGEYANNNNQTPLKVTHMHKPPQTVTNQRVSFNNLFHFHDLSTETRSFLRGIDGNTPLPFMAATADDENGVSSPNSTVSSISGKRSEREGNGDENDAVERGSCSRGGGSDDDDGGGCGGDGDSSRKKLRLSKEQSMLLEETFKEHNTLNPKQKQALAKQLNLKPRQVEVWFQNRRARTKLKQTEVDCEYLKRCCETLTDENRRLQKEVQELRALKLSPQLYMQMNPPTTLTMCPSCERVAVSSASSSSAAAAALPSASASPSGPSVQRPTPVNPWAAMSIQNRSRP