Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061381.t1 | AES77954.1 | 90 | 239 | 24 | 0 | 1 | 239 | 1 | 239 | 2.10E-117 | 431.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061381.t1 | Q39261 | 36.9 | 84 | 45 | 3 | 87 | 170 | 52 | 127 | 4.8e-06 | 53.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061381.t1 | G7KUQ7 | 90.0 | 239 | 24 | 0 | 1 | 239 | 1 | 239 | 1.5e-117 | 431.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene061381.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061381.t1 | MTR_7g022250 | 89.958 | 239 | 24 | 0 | 1 | 239 | 1 | 239 | 2.02e-162 | 449 |
| MS.gene061381.t1 | MTR_7g028890 | 69.038 | 239 | 55 | 3 | 1 | 239 | 1 | 220 | 1.17e-116 | 332 |
| MS.gene061381.t1 | MTR_7g028915 | 68.201 | 239 | 49 | 3 | 1 | 239 | 1 | 212 | 9.40e-111 | 317 |
| MS.gene061381.t1 | MTR_7g022270 | 67.005 | 197 | 28 | 3 | 24 | 219 | 1 | 161 | 1.92e-87 | 256 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 50 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGGGAGACTTTGAATTCT+TGG | 0.167821 | 7.1:-76616807 | None:intergenic |
| AATATGACTGAGATTGAATT+TGG | 0.189345 | 7.1:+76617268 | MS.gene061381:CDS |
| TTGAATTCTTGGTTTCAAAA+TGG | 0.201803 | 7.1:-76616796 | None:intergenic |
| CTTGAGTCTCATTGTCTTCC+TGG | 0.219987 | 7.1:-76617330 | None:intergenic |
| ATTCTTCATCATCTTCAAAT+TGG | 0.223485 | 7.1:-76616729 | None:intergenic |
| CTCCTTCCCCTTGTTCTCAT+TGG | 0.229008 | 7.1:-76616684 | None:intergenic |
| CAAATTTGTGAGAACTTTGA+TGG | 0.256763 | 7.1:-76616985 | None:intergenic |
| AAGAAATGGAGGAATTTCTT+TGG | 0.278435 | 7.1:-76616864 | None:intergenic |
| TGAATTCTTGGTTTCAAAAT+GGG | 0.333972 | 7.1:-76616795 | None:intergenic |
| ACATATAGATGAAGTGAATA+AGG | 0.341762 | 7.1:+76617216 | MS.gene061381:CDS |
| CACTGCCACTCCTTTCCATA+TGG | 0.342482 | 7.1:+76617093 | MS.gene061381:CDS |
| AGAAATGGAGGAATTTCTTT+GGG | 0.353550 | 7.1:-76616863 | None:intergenic |
| CTTAAGGGTCAAATCAATCT+TGG | 0.361873 | 7.1:-76617365 | None:intergenic |
| AATATATAGATCAAAGCTTA+AGG | 0.366337 | 7.1:-76617381 | None:intergenic |
| TCCAAGCTTCGTTAAAAGCT+TGG | 0.373123 | 7.1:-76617033 | None:intergenic |
| AATTACTCAAATGGTAATCA+AGG | 0.414383 | 7.1:+76617058 | MS.gene061381:CDS |
| ACTCCTTTCCATATGGGTTG+TGG | 0.424172 | 7.1:+76617100 | MS.gene061381:CDS |
| TTCACTACTCCACGTGCATT+AGG | 0.424339 | 7.1:+76616959 | MS.gene061381:CDS |
| AACAATATGATTCAGCATGC+AGG | 0.425567 | 7.1:+76617142 | MS.gene061381:CDS |
| ATTGATATCCACAACCCATA+TGG | 0.434960 | 7.1:-76617108 | None:intergenic |
| GCTTGGATAAATTACTCAAA+TGG | 0.446981 | 7.1:+76617049 | MS.gene061381:CDS |
| TCACAAATTTGAGAGAAGTC+TGG | 0.482572 | 7.1:+76616997 | MS.gene061381:CDS |
| ACTGCCACTCCTTTCCATAT+GGG | 0.486001 | 7.1:+76617094 | MS.gene061381:CDS |
| TTATTCACTTCATCTATATG+TGG | 0.494069 | 7.1:-76617214 | None:intergenic |
| TTAAGGGTCAAATCAATCTT+GGG | 0.500241 | 7.1:-76617364 | None:intergenic |
| ATATATAGATCAAAGCTTAA+GGG | 0.520066 | 7.1:-76617380 | None:intergenic |
| TGAATTTGGTTCAATTTCAG+AGG | 0.531449 | 7.1:+76617282 | MS.gene061381:CDS |
| TTGGATTCATTGTTGATCAG+AGG | 0.552579 | 7.1:+76616891 | MS.gene061381:CDS |
| GAAGTGAATAAGGCTGATCA+AGG | 0.555258 | 7.1:+76617226 | MS.gene061381:CDS |
| TTCATTGTTGATCAGAGGAA+AGG | 0.555662 | 7.1:+76616896 | MS.gene061381:CDS |
| ACAATATGATTCAGCATGCA+GGG | 0.561522 | 7.1:+76617143 | MS.gene061381:CDS |
| GTGCAACACCAATGAGAACA+AGG | 0.569603 | 7.1:+76616676 | MS.gene061381:CDS |
| CAAGCGAAAGGCCATCGTGA+TGG | 0.579476 | 7.1:+76616766 | MS.gene061381:CDS |
| GAATTTGGTTCAATTTCAGA+GGG | 0.586650 | 7.1:+76617283 | MS.gene061381:CDS |
| ATTACTCAAATGGTAATCAA+GGG | 0.589859 | 7.1:+76617059 | MS.gene061381:CDS |
| TGTTAGTGACAAAGTTGACC+AGG | 0.590985 | 7.1:+76617312 | MS.gene061381:CDS |
| TGAGAACAAGGGGAAGGAGA+AGG | 0.594646 | 7.1:+76616688 | MS.gene061381:CDS |
| TTTGGTTCAATTTCAGAGGG+AGG | 0.596815 | 7.1:+76617286 | MS.gene061381:CDS |
| CACCAATGAGAACAAGGGGA+AGG | 0.620535 | 7.1:+76616682 | MS.gene061381:CDS |
| TATCCACAACCCATATGGAA+AGG | 0.633718 | 7.1:-76617103 | None:intergenic |
| GGAAGACAATGAGACTCAAG+AGG | 0.638438 | 7.1:+76617333 | MS.gene061381:CDS |
| ACTACTCCACGTGCATTAGG+TGG | 0.640042 | 7.1:+76616962 | MS.gene061381:CDS |
| TCATCTATATGTGGAATATG+CGG | 0.642794 | 7.1:-76617205 | None:intergenic |
| GCAACACCAATGAGAACAAG+GGG | 0.642885 | 7.1:+76616678 | MS.gene061381:CDS |
| TGCAACACCAATGAGAACAA+GGG | 0.650590 | 7.1:+76616677 | MS.gene061381:CDS |
| GAACAATGTGAACAAGCGAA+AGG | 0.655169 | 7.1:+76616754 | MS.gene061381:CDS |
| ACAACCCATATGGAAAGGAG+TGG | 0.680303 | 7.1:-76617098 | None:intergenic |
| AGACAATGAGACTCAAGAGG+AGG | 0.680897 | 7.1:+76617336 | MS.gene061381:CDS |
| ATGGGATTCTTCCATCACGA+TGG | 0.687731 | 7.1:-76616777 | None:intergenic |
| TGATGGCCACCTAATGCACG+TGG | 0.714086 | 7.1:-76616968 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCTTCAACAATATCTTTTT+GGG | - | chr7.1:76616828-76616847 | None:intergenic | 20.0% |
| ! | AATATGACTGAGATTGAATT+TGG | + | chr7.1:76617268-76617287 | MS.gene061381:CDS | 25.0% |
| ! | AATTACTCAAATGGTAATCA+AGG | + | chr7.1:76617058-76617077 | MS.gene061381:CDS | 25.0% |
| ! | ACATATAGATGAAGTGAATA+AGG | + | chr7.1:76617216-76617235 | MS.gene061381:CDS | 25.0% |
| ! | ATGAATCCAAAAAGAAGAAA+TGG | - | chr7.1:76616881-76616900 | None:intergenic | 25.0% |
| ! | ATTACTCAAATGGTAATCAA+GGG | + | chr7.1:76617059-76617078 | MS.gene061381:CDS | 25.0% |
| ! | ATTCTTCATCATCTTCAAAT+TGG | - | chr7.1:76616732-76616751 | None:intergenic | 25.0% |
| ! | TGAATTCTTGGTTTCAAAAT+GGG | - | chr7.1:76616798-76616817 | None:intergenic | 25.0% |
| ! | TTATTCACTTCATCTATATG+TGG | - | chr7.1:76617217-76617236 | None:intergenic | 25.0% |
| ! | TTGAATTCTTGGTTTCAAAA+TGG | - | chr7.1:76616799-76616818 | None:intergenic | 25.0% |
| !! | CTTCTTCAACAATATCTTTT+TGG | - | chr7.1:76616829-76616848 | None:intergenic | 25.0% |
| !! | TCTTCAACAATATCTTTTTG+GGG | - | chr7.1:76616827-76616846 | None:intergenic | 25.0% |
| !!! | TTTTATGCAGGAAATTTGTT+TGG | + | chr7.1:76617175-76617194 | MS.gene061381:CDS | 25.0% |
| AAGAAATGGAGGAATTTCTT+TGG | - | chr7.1:76616867-76616886 | None:intergenic | 30.0% | |
| AATCCAAAAAGAAGAAATGG+AGG | - | chr7.1:76616878-76616897 | None:intergenic | 30.0% | |
| AGAAATGGAGGAATTTCTTT+GGG | - | chr7.1:76616866-76616885 | None:intergenic | 30.0% | |
| CAAATTTGTGAGAACTTTGA+TGG | - | chr7.1:76616988-76617007 | None:intergenic | 30.0% | |
| GCTTGGATAAATTACTCAAA+TGG | + | chr7.1:76617049-76617068 | MS.gene061381:CDS | 30.0% | |
| TCATCTATATGTGGAATATG+CGG | - | chr7.1:76617208-76617227 | None:intergenic | 30.0% | |
| TTAAGGGTCAAATCAATCTT+GGG | - | chr7.1:76617367-76617386 | None:intergenic | 30.0% | |
| ! | ATTCCTCCATTTCTTCTTTT+TGG | + | chr7.1:76616872-76616891 | MS.gene061381:CDS | 30.0% |
| ! | GAATTTGGTTCAATTTCAGA+GGG | + | chr7.1:76617283-76617302 | MS.gene061381:CDS | 30.0% |
| ! | TGAATTTGGTTCAATTTCAG+AGG | + | chr7.1:76617282-76617301 | MS.gene061381:CDS | 30.0% |
| AACAATATGATTCAGCATGC+AGG | + | chr7.1:76617142-76617161 | MS.gene061381:CDS | 35.0% | |
| ACAATATGATTCAGCATGCA+GGG | + | chr7.1:76617143-76617162 | MS.gene061381:CDS | 35.0% | |
| AGAGAAGTCTGGTCAAAAAA+AGG | + | chr7.1:76617008-76617027 | MS.gene061381:CDS | 35.0% | |
| ATTGATATCCACAACCCATA+TGG | - | chr7.1:76617111-76617130 | None:intergenic | 35.0% | |
| CTTAAGGGTCAAATCAATCT+TGG | - | chr7.1:76617368-76617387 | None:intergenic | 35.0% | |
| TCACAAATTTGAGAGAAGTC+TGG | + | chr7.1:76616997-76617016 | MS.gene061381:CDS | 35.0% | |
| TTCATTGTTGATCAGAGGAA+AGG | + | chr7.1:76616896-76616915 | MS.gene061381:CDS | 35.0% | |
| TTGGATTCATTGTTGATCAG+AGG | + | chr7.1:76616891-76616910 | MS.gene061381:CDS | 35.0% | |
| GAACAATGTGAACAAGCGAA+AGG | + | chr7.1:76616754-76616773 | MS.gene061381:CDS | 40.0% | |
| GAAGTGAATAAGGCTGATCA+AGG | + | chr7.1:76617226-76617245 | MS.gene061381:CDS | 40.0% | |
| TATCCACAACCCATATGGAA+AGG | - | chr7.1:76617106-76617125 | None:intergenic | 40.0% | |
| TCCAAGCTTCGTTAAAAGCT+TGG | - | chr7.1:76617036-76617055 | None:intergenic | 40.0% | |
| TGCAACACCAATGAGAACAA+GGG | + | chr7.1:76616677-76616696 | MS.gene061381:CDS | 40.0% | |
| TGTTAGTGACAAAGTTGACC+AGG | + | chr7.1:76617312-76617331 | MS.gene061381:CDS | 40.0% | |
| TTGGGGAGACTTTGAATTCT+TGG | - | chr7.1:76616810-76616829 | None:intergenic | 40.0% | |
| ! | GGGTCATCAAACTTTTATGC+AGG | + | chr7.1:76617163-76617182 | MS.gene061381:CDS | 40.0% |
| ! | TCCAAGCTTTTAACGAAGCT+TGG | + | chr7.1:76617032-76617051 | MS.gene061381:CDS | 40.0% |
| ! | TTTGGTTCAATTTCAGAGGG+AGG | + | chr7.1:76617286-76617305 | MS.gene061381:CDS | 40.0% |
| ACAACCCATATGGAAAGGAG+TGG | - | chr7.1:76617101-76617120 | None:intergenic | 45.0% | |
| ACTGCCACTCCTTTCCATAT+GGG | + | chr7.1:76617094-76617113 | MS.gene061381:CDS | 45.0% | |
| AGACAATGAGACTCAAGAGG+AGG | + | chr7.1:76617336-76617355 | MS.gene061381:CDS | 45.0% | |
| ATGGGATTCTTCCATCACGA+TGG | - | chr7.1:76616780-76616799 | None:intergenic | 45.0% | |
| CTTGAGTCTCATTGTCTTCC+TGG | - | chr7.1:76617333-76617352 | None:intergenic | 45.0% | |
| GCAACACCAATGAGAACAAG+GGG | + | chr7.1:76616678-76616697 | MS.gene061381:CDS | 45.0% | |
| GGAAGACAATGAGACTCAAG+AGG | + | chr7.1:76617333-76617352 | MS.gene061381:CDS | 45.0% | |
| GTGCAACACCAATGAGAACA+AGG | + | chr7.1:76616676-76616695 | MS.gene061381:CDS | 45.0% | |
| TTCACTACTCCACGTGCATT+AGG | + | chr7.1:76616959-76616978 | MS.gene061381:CDS | 45.0% | |
| ! | ACTCCTTTCCATATGGGTTG+TGG | + | chr7.1:76617100-76617119 | MS.gene061381:CDS | 45.0% |
| ACTACTCCACGTGCATTAGG+TGG | + | chr7.1:76616962-76616981 | MS.gene061381:CDS | 50.0% | |
| CACCAATGAGAACAAGGGGA+AGG | + | chr7.1:76616682-76616701 | MS.gene061381:CDS | 50.0% | |
| CACTGCCACTCCTTTCCATA+TGG | + | chr7.1:76617093-76617112 | MS.gene061381:CDS | 50.0% | |
| CTCCTTCCCCTTGTTCTCAT+TGG | - | chr7.1:76616687-76616706 | None:intergenic | 50.0% | |
| TGAGAACAAGGGGAAGGAGA+AGG | + | chr7.1:76616688-76616707 | MS.gene061381:CDS | 50.0% | |
| CAAGCGAAAGGCCATCGTGA+TGG | + | chr7.1:76616766-76616785 | MS.gene061381:CDS | 55.0% | |
| TGATGGCCACCTAATGCACG+TGG | - | chr7.1:76616971-76616990 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 76616674 | 76617393 | 76616674 | ID=MS.gene061381 |
| chr7.1 | mRNA | 76616674 | 76617393 | 76616674 | ID=MS.gene061381.t1;Parent=MS.gene061381 |
| chr7.1 | exon | 76616674 | 76617393 | 76616674 | ID=MS.gene061381.t1.exon1;Parent=MS.gene061381.t1 |
| chr7.1 | CDS | 76616674 | 76617393 | 76616674 | ID=cds.MS.gene061381.t1;Parent=MS.gene061381.t1 |
| Gene Sequence |
| Protein sequence |
