AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene06160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06160.t1 MTR_8g102100 94.010 384 15 3 1 379 1 381 0.0 716
MS.gene06160.t1 MTR_6g090380 55.366 410 112 8 1 373 1 376 2.51e-141 407
MS.gene06160.t1 MTR_3g103960 44.242 330 114 9 46 374 18 278 7.72e-77 239
MS.gene06160.t1 MTR_4g078660 71.818 110 31 0 75 184 54 163 8.03e-58 191
MS.gene06160.t1 MTR_2g090605 74.757 103 26 0 81 183 77 179 5.92e-57 191
MS.gene06160.t1 MTR_7g092070 72.897 107 29 0 77 183 260 366 4.53e-54 190
MS.gene06160.t1 MTR_5g071070 46.025 239 97 9 77 283 144 382 2.75e-53 186
MS.gene06160.t1 MTR_5g071070 46.025 239 97 9 77 283 226 464 9.20e-53 186
MS.gene06160.t1 MTR_3g029590 51.515 165 70 2 77 235 260 420 1.88e-51 183
MS.gene06160.t1 MTR_5g082830 54.037 161 70 1 77 237 213 369 1.31e-50 180
MS.gene06160.t1 MTR_3g435680 66.379 116 38 1 68 183 139 253 1.79e-50 174
MS.gene06160.t1 MTR_1g050438 63.551 107 39 0 77 183 224 330 1.12e-48 173
MS.gene06160.t1 MTR_0728s0020 63.551 107 39 0 77 183 240 346 1.84e-48 173
MS.gene06160.t1 MTR_1g075890 67.290 107 35 0 77 183 225 331 3.14e-47 170
MS.gene06160.t1 MTR_3g070880 49.693 163 54 3 77 237 159 295 3.17e-47 168
MS.gene06160.t1 MTR_3g435590 47.024 168 78 4 77 243 234 391 8.90e-47 166
MS.gene06160.t1 MTR_3g464270 46.914 162 75 4 79 238 7 159 4.54e-40 145
MS.gene06160.t1 MTR_3g464260 68.056 72 23 0 112 183 212 283 1.10e-29 120
MS.gene06160.t1 MTR_3g464290 60.759 79 30 1 77 154 96 174 4.74e-29 117
MS.gene06160.t1 MTR_3g464280 42.991 107 27 1 77 183 184 256 1.07e-20 94.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene06160.t1 AT4G29190 54.308 383 137 14 1 373 1 355 4.00e-126 367
MS.gene06160.t1 AT2G19810 51.309 382 153 10 1 373 1 358 5.31e-114 337
MS.gene06160.t1 AT2G25900 44.904 363 138 9 1 350 1 314 1.67e-90 275
MS.gene06160.t1 AT2G25900 44.904 363 138 9 1 350 1 314 1.67e-90 275
MS.gene06160.t1 AT1G03790 63.433 134 48 1 52 184 53 186 4.54e-58 194
MS.gene06160.t1 AT5G44260 68.548 124 30 2 62 184 49 164 2.09e-55 187
MS.gene06160.t1 AT2G41900 71.028 107 31 0 77 183 257 363 3.40e-53 187
MS.gene06160.t1 AT5G12850 71.963 107 30 0 77 183 253 359 4.90e-53 187
MS.gene06160.t1 AT3G55980 46.829 205 94 4 77 278 207 399 1.23e-51 181
MS.gene06160.t1 AT3G55980 46.829 205 94 4 77 278 274 466 3.14e-51 181
MS.gene06160.t1 AT5G58620 69.159 107 33 0 77 183 205 311 7.20e-51 179
MS.gene06160.t1 AT5G07500 63.333 120 42 1 66 183 35 154 9.26e-51 170
MS.gene06160.t1 AT2G40140 53.459 159 69 1 77 235 210 363 1.04e-48 174
MS.gene06160.t1 AT2G40140 53.459 159 69 1 77 235 210 363 1.04e-48 174

Find 123 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGCTTGTGAGCCCTTTCT+TGG 0.247364 4.2:+3197459 MS.gene06160:CDS
GGGTCGGATCACGGGTCCTC+TGG 0.290200 4.2:-3197206 None:intergenic
AATGGATCGGGTTCAGGTTC+GGG 0.301747 4.2:+3197400 MS.gene06160:CDS
TGGAGTCTGGAAGAGATATA+AGG 0.307621 4.2:+3197338 MS.gene06160:CDS
TCCGGATGTTGGGTGGGTTT+CGG 0.313054 4.2:+3197438 MS.gene06160:CDS
CGTTGGTAGGCTACAGAATC+CGG 0.324864 4.2:-3197227 None:intergenic
CAACTTCCTTTACGAAAATC+AGG 0.327092 4.2:-3196731 None:intergenic
CTCACACGGCGGAGCAACTT+AGG 0.327174 4.2:+3196876 MS.gene06160:CDS
CACACCGCCGCCATGATGTT+AGG 0.327724 4.2:+3196338 None:intergenic
TGAAGTCATTACCTTCTTCA+TGG 0.329074 4.2:+3197149 MS.gene06160:CDS
TTCTCGCCGGGATGAGAAAA+AGG 0.329763 4.2:-3196659 None:intergenic
CCGGAGTAGTTATACTTTCT+CGG 0.332203 4.2:-3196698 None:intergenic
TAATGGATCGGGTTCAGGTT+CGG 0.338161 4.2:+3197399 MS.gene06160:CDS
CGCATGGTGTGTTCGAGTGC+TGG 0.340661 4.2:+3196780 MS.gene06160:CDS
TAATCGTCGTTGGAGGTTAA+AGG 0.353804 4.2:-3196443 None:intergenic
GCCGTTGGATTGTTCATTGT+TGG 0.354051 4.2:-3196407 None:intergenic
GACCGAGTCATCGGAGTAAT+CGG 0.354759 4.2:-3197052 None:intergenic
CAAATGGGTTCTCCTAGATT+CGG 0.355364 4.2:+3197178 MS.gene06160:CDS
TTCTTCATGGAATGTTCAAA+TGG 0.360051 4.2:+3197162 MS.gene06160:CDS
TACTCCGATGACTCGGTCCT+TGG 0.371166 4.2:+3197057 MS.gene06160:CDS
CGTATGACGGTTCTCCTCTC+AGG 0.385313 4.2:+3196942 MS.gene06160:CDS
CGTAAAGGAAGTTGCAAGAA+AGG 0.403608 4.2:+3196740 MS.gene06160:CDS
ACAAACTGGCCGGCGACAAC+TGG 0.411554 4.2:-3196846 None:intergenic
TTGTTCATTGTTGGCCATGC+TGG 0.418920 4.2:-3196398 None:intergenic
TGAAGAAGGTAATGACTTCA+TGG 0.429121 4.2:-3197146 None:intergenic
TCCTCCTGGATCGGTTTCTC+CGG 0.431746 4.2:+3197009 MS.gene06160:CDS
GAGCTTGTGAGCCCTTTCTT+GGG 0.435024 4.2:+3197460 MS.gene06160:CDS
ACACCGCCGCCATGATGTTA+GGG 0.445475 4.2:+3196339 None:intergenic
CGGAGTAGTTATACTTTCTC+GGG 0.448222 4.2:-3196697 None:intergenic
CCTCTGGGAGACCCGAATCT+AGG 0.450232 4.2:-3197190 None:intergenic
GTGATGGAGAGGGTGGAGTC+TGG 0.450842 4.2:+3197325 MS.gene06160:CDS
GGGTGAAGTGGTTGAAGATC+CGG 0.455522 4.2:+3197420 MS.gene06160:CDS
CAGAATCCGGGTCGGATCAC+GGG 0.457628 4.2:-3197214 None:intergenic
GGAGTCTGGAAGAGATATAA+GGG 0.462144 4.2:+3197339 MS.gene06160:CDS
AACATTCAATCACCCAAGAA+AGG 0.463244 4.2:-3197472 None:intergenic
TTCGGCGATGCGCACGTGGC+AGG 0.465958 4.2:+3196615 MS.gene06160:CDS
CTTCCGTTCATGTCTCCTCC+TGG 0.466787 4.2:+3196995 MS.gene06160:CDS
TCCGAAACCCACCCAACATC+CGG 0.467976 4.2:-3197439 None:intergenic
ATCAACGCAGTGCACACTCC+GGG 0.469752 4.2:-3196918 None:intergenic
GTTCATGTCTCCTCCTGGAT+CGG 0.471938 4.2:+3197000 MS.gene06160:CDS
TCACACGGCGGAGCAACTTA+GGG 0.475617 4.2:+3196877 MS.gene06160:CDS
ACTCCGATGACTCGGTCCTT+GGG 0.476813 4.2:+3197058 MS.gene06160:CDS
ATTTGAACATTCCATGAAGA+AGG 0.481692 4.2:-3197160 None:intergenic
CGGCGGCGAGCTTTCTCGCC+GGG 0.483303 4.2:-3196671 None:intergenic
CGCAGGTGCCGAGTCGTGGT+AGG 0.483561 4.2:+3197257 MS.gene06160:CDS
GGTCGGATCACGGGTCCTCT+GGG 0.485156 4.2:-3197205 None:intergenic
CGTCTTTACACGGCTGAGTA+CGG 0.485485 4.2:-3196820 None:intergenic
TCTTCATGGAATGTTCAAAT+GGG 0.500081 4.2:+3197163 MS.gene06160:CDS
GACTCCACCCTCTCCATCAC+AGG 0.508923 4.2:-3197322 None:intergenic
CTCCGACTCAACAGAGTCCC+CGG 0.512371 4.2:+3196900 MS.gene06160:CDS
TCGGTCCTTGGGAAGATCTG+TGG 0.513782 4.2:+3197069 MS.gene06160:CDS
TCCACCGGAGAAACCGATCC+AGG 0.514556 4.2:-3197013 None:intergenic
TGGTTGAAGATCCGGATGTT+GGG 0.514839 4.2:+3197428 MS.gene06160:CDS
ACAGAATCCGGGTCGGATCA+CGG 0.517668 4.2:-3197215 None:intergenic
CCTGCGTAGGCGTCGTTGGT+AGG 0.518542 4.2:-3197240 None:intergenic
TTCTAGCTCTGTGAATGAAA+TGG 0.520762 4.2:+3197093 MS.gene06160:CDS
TCCTGGATCGGTTTCTCCGG+TGG 0.521510 4.2:+3197012 MS.gene06160:CDS
ACGGCGGCGAGCTTTCTCGC+CGG 0.522012 4.2:-3196672 None:intergenic
CGGTCCTTGGGAAGATCTGT+GGG 0.525368 4.2:+3197070 MS.gene06160:CDS
TTGAAGATCCGGATGTTGGG+TGG 0.525953 4.2:+3197431 MS.gene06160:CDS
CACGTGGCAGGTCACATGAC+TGG 0.526588 4.2:+3196627 MS.gene06160:CDS
CTTCCCAAGGACCGAGTCAT+CGG 0.527368 4.2:-3197061 None:intergenic
AAAATCAGGACACGAAGTGC+CGG 0.531042 4.2:-3196717 None:intergenic
GCATGTTCGAGTTTAAGATT+CGG 0.531245 4.2:+3196597 MS.gene06160:CDS
ACATTCAATCACCCAAGAAA+GGG 0.534836 4.2:-3197471 None:intergenic
CTCCGGGGACTCTGTTGAGT+CGG 0.537123 4.2:-3196902 None:intergenic
AAATGGGTTCTCCTAGATTC+GGG 0.537187 4.2:+3197179 MS.gene06160:CDS
AGTTATACTTTCTCGGGTCA+CGG 0.538971 4.2:-3196691 None:intergenic
GGCACCTGCGTAGGCGTCGT+TGG 0.553486 4.2:-3197244 None:intergenic
CAACTGGTTCCGTCTTTACA+CGG 0.558745 4.2:-3196830 None:intergenic
CGTACTCAGCCGTGTAAAGA+CGG 0.559308 4.2:+3196821 MS.gene06160:CDS
TGAAGATCCGGATGTTGGGT+GGG 0.561336 4.2:+3197432 MS.gene06160:CDS
CTCCGATTACTCCGATGACT+CGG 0.561730 4.2:+3197050 MS.gene06160:CDS
GGGTTCAGGTTCGGGTGAAG+TGG 0.562356 4.2:+3197408 MS.gene06160:CDS
GTGGTTGAAGATCCGGATGT+TGG 0.565014 4.2:+3197427 MS.gene06160:CDS
AGGTGGTTCCCCTAACATCA+TGG 0.567452 4.2:-3196348 None:intergenic
GACTCAATAGCAAGCCTGAG+AGG 0.570639 4.2:-3196956 None:intergenic
AGAGCCTGTGATGGAGAGGG+TGG 0.580941 4.2:+3197318 MS.gene06160:CDS
GTTGGATTTGTACGATGAGG+TGG 0.581590 4.2:-3196365 None:intergenic
TGGCCATGCTGGAACGTGTA+CGG 0.582344 4.2:-3196387 None:intergenic
AATCAACGCAGTGCACACTC+CGG 0.582408 4.2:-3196919 None:intergenic
TCTCTGAGAAACTTACAACT+TGG 0.583296 4.2:+3197121 MS.gene06160:CDS
AGGAAGAGCCTGTGATGGAG+AGG 0.585551 4.2:+3197314 MS.gene06160:CDS
CACTGCGTTGATTCGTATGA+CGG 0.588289 4.2:+3196929 MS.gene06160:CDS
CCTACGCAGGTGCCGAGTCG+TGG 0.591860 4.2:+3197253 MS.gene06160:CDS
CTGAGGATAATCGTCGTTGG+AGG 0.595113 4.2:-3196450 None:intergenic
CGACGACACCGATTCCGACT+CGG 0.598832 4.2:+3196523 MS.gene06160:CDS
AGAGGACCCGTGATCCGACC+CGG 0.601883 4.2:+3197208 MS.gene06160:CDS
GTTGGTAGGCTACAGAATCC+GGG 0.603145 4.2:-3197226 None:intergenic
TTGCGAGGAAGAGCCTGTGA+TGG 0.605419 4.2:+3197309 MS.gene06160:CDS
TGATTAACCCTACCACGACT+CGG 0.606063 4.2:-3197265 None:intergenic
CCACGACTCGGCACCTGCGT+AGG 0.607212 4.2:-3197253 None:intergenic
GCAGGTGCCGAGTCGTGGTA+GGG 0.610663 4.2:+3197258 MS.gene06160:CDS
GAACTGAGGATAATCGTCGT+TGG 0.611416 4.2:-3196453 None:intergenic
CACCGCCGCCATGATGTTAG+GGG 0.612627 4.2:+3196340 None:intergenic
AAGACGGAACCAGTTGTCGC+CGG 0.615571 4.2:+3196837 MS.gene06160:CDS
AAGATTCGGCGATGCGCACG+TGG 0.619291 4.2:+3196611 MS.gene06160:CDS
GAATCCACCGATTCATGACT+CGG 0.619620 4.2:-3196554 None:intergenic
TAGCTCTGTGAATGAAATGG+TGG 0.623336 4.2:+3197096 MS.gene06160:CDS
CCGAGAAAGTATAACTACTC+CGG 0.623467 4.2:+3196698 MS.gene06160:CDS
GGAAGAGCCTGTGATGGAGA+GGG 0.627453 4.2:+3197315 MS.gene06160:CDS
GATTCGTGTGAGTTTGCGCA+TGG 0.630448 4.2:+3196764 MS.gene06160:CDS
ATCGGAGTAATCGGAGACAT+CGG 0.633731 4.2:-3197043 None:intergenic
GGCTGAGTACGGTAACGAGA+AGG 0.636999 4.2:-3196809 None:intergenic
CCTACCAACGACGCCTACGC+AGG 0.637663 4.2:+3197240 MS.gene06160:CDS
GCCAACAATGAACAATCCAA+CGG 0.640124 4.2:+3196406 MS.gene06160:CDS
GGAGTAATCGGAGACATCGG+AGG 0.641390 4.2:-3197040 None:intergenic
TGGCAGGTCACATGACTGGA+CGG 0.644811 4.2:+3196631 MS.gene06160:CDS
CAACCGTACACGTTCCAGCA+TGG 0.649929 4.2:+3196384 MS.gene06160:CDS
CATGCTGGAACGTGTACGGT+TGG 0.652730 4.2:-3196383 None:intergenic
TCAACGCAGTGCACACTCCG+GGG 0.673364 4.2:-3196917 None:intergenic
GACATCGGAGGAGACTCCAC+CGG 0.675579 4.2:-3197028 None:intergenic
CCTAGATTCGGGTCTCCCAG+AGG 0.678542 4.2:+3197190 MS.gene06160:CDS
GATCCAGGAGGAGACATGAA+CGG 0.679198 4.2:-3196998 None:intergenic
GAATCTTAAACTCGAACATG+CGG 0.686731 4.2:-3196595 None:intergenic
GCTTCTTGCATGTAGAACTG+AGG 0.687997 4.2:-3196467 None:intergenic
AACAATGAACAATCCAACGG+CGG 0.692559 4.2:+3196409 MS.gene06160:CDS
ACCGGAGAAACCGATCCAGG+AGG 0.697000 4.2:-3197010 None:intergenic
GTAGGCTACAGAATCCGGGT+CGG 0.700260 4.2:-3197222 None:intergenic
AGAACCCACAGATCTTCCCA+AGG 0.706781 4.2:-3197074 None:intergenic
ACGGTTGGATTTGTACGATG+AGG 0.707900 4.2:-3196368 None:intergenic
TCTTTGGGATCAGTGTTGCG+AGG 0.716443 4.2:+3197294 MS.gene06160:CDS
TATACTTTCTCGGGTCACGG+CGG 0.737764 4.2:-3196688 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GGTTAATCATTTTGATCTTT+GGG + chr4.2:3197279-3197298 MS.gene06160:CDS 25.0%
ATTTGAACATTCCATGAAGA+AGG - chr4.2:3197163-3197182 None:intergenic 30.0%
TCTTCATGGAATGTTCAAAT+GGG + chr4.2:3197163-3197182 MS.gene06160:CDS 30.0%
TTCTTCATGGAATGTTCAAA+TGG + chr4.2:3197162-3197181 MS.gene06160:CDS 30.0%
! AGCAAAGAGAACTCTTTTAA+TGG + chr4.2:3197382-3197401 MS.gene06160:CDS 30.0%
!! AGAGAACTCTTTTAATGGAT+CGG + chr4.2:3197387-3197406 MS.gene06160:CDS 30.0%
!! GGGTTAATCATTTTGATCTT+TGG + chr4.2:3197278-3197297 MS.gene06160:CDS 30.0%
CAACTTCCTTTACGAAAATC+AGG - chr4.2:3196734-3196753 None:intergenic 35.0%
GAATCTTAAACTCGAACATG+CGG - chr4.2:3196598-3196617 None:intergenic 35.0%
TGAAGAAGGTAATGACTTCA+TGG - chr4.2:3197149-3197168 None:intergenic 35.0%
TGAAGTCATTACCTTCTTCA+TGG + chr4.2:3197149-3197168 MS.gene06160:CDS 35.0%
! CGTTTTCGTTAACATAGTGT+TGG - chr4.2:3196505-3196524 None:intergenic 35.0%
! GATTTTTCCGAGTCATGAAT+CGG + chr4.2:3196547-3196566 MS.gene06160:CDS 35.0%
! GCATGTTCGAGTTTAAGATT+CGG + chr4.2:3196597-3196616 MS.gene06160:CDS 35.0%
! TCTCTGAGAAACTTACAACT+TGG + chr4.2:3197121-3197140 MS.gene06160:CDS 35.0%
!! GAGAACTCTTTTAATGGATC+GGG + chr4.2:3197388-3197407 MS.gene06160:CDS 35.0%
!! TTCTAGCTCTGTGAATGAAA+TGG + chr4.2:3197093-3197112 MS.gene06160:CDS 35.0%
!!! AGTTTGTTTTTTCGCTCACA+CGG + chr4.2:3196862-3196881 MS.gene06160:CDS 35.0%
AAAAATCTCCGAGTCGGAAT+CGG - chr4.2:3196534-3196553 None:intergenic 40.0%
AACAATGAACAATCCAACGG+CGG + chr4.2:3196409-3196428 MS.gene06160:CDS 40.0%
AGTTATACTTTCTCGGGTCA+CGG - chr4.2:3196694-3196713 None:intergenic 40.0%
CCGGAGTAGTTATACTTTCT+CGG - chr4.2:3196701-3196720 None:intergenic 40.0%
CGGAGTAGTTATACTTTCTC+GGG - chr4.2:3196700-3196719 None:intergenic 40.0%
CGTAAAGGAAGTTGCAAGAA+AGG + chr4.2:3196740-3196759 MS.gene06160:CDS 40.0%
GCCAACAATGAACAATCCAA+CGG + chr4.2:3196406-3196425 MS.gene06160:CDS 40.0%
GGAGTCTGGAAGAGATATAA+GGG + chr4.2:3197339-3197358 MS.gene06160:CDS 40.0%
GTGTGAGCGAAAAAACAAAC+TGG - chr4.2:3196863-3196882 None:intergenic 40.0%
TAATCGTCGTTGGAGGTTAA+AGG - chr4.2:3196446-3196465 None:intergenic 40.0%
TGGAGTCTGGAAGAGATATA+AGG + chr4.2:3197338-3197357 MS.gene06160:CDS 40.0%
! AAATGGGTTCTCCTAGATTC+GGG + chr4.2:3197179-3197198 MS.gene06160:CDS 40.0%
! CAAATGGGTTCTCCTAGATT+CGG + chr4.2:3197178-3197197 MS.gene06160:CDS 40.0%
! CCGAGAAAGTATAACTACTC+CGG + chr4.2:3196698-3196717 MS.gene06160:CDS 40.0%
! TAGCTCTGTGAATGAAATGG+TGG + chr4.2:3197096-3197115 MS.gene06160:CDS 40.0%
! TCGTGTCCTGATTTTCGTAA+AGG + chr4.2:3196725-3196744 MS.gene06160:CDS 40.0%
!! TCTTTTAATGGATCGGGTTC+AGG + chr4.2:3197394-3197413 MS.gene06160:CDS 40.0%
AAAATCAGGACACGAAGTGC+CGG - chr4.2:3196720-3196739 None:intergenic 45.0%
ACGGTTGGATTTGTACGATG+AGG - chr4.2:3196371-3196390 None:intergenic 45.0%
ACTCGGAAAAATCTCCGAGT+CGG - chr4.2:3196540-3196559 None:intergenic 45.0%
ATCGGAGTAATCGGAGACAT+CGG - chr4.2:3197046-3197065 None:intergenic 45.0%
GAACTGAGGATAATCGTCGT+TGG - chr4.2:3196456-3196475 None:intergenic 45.0%
GAGCGAAAAAACAAACTGGC+CGG - chr4.2:3196859-3196878 None:intergenic 45.0%
GCCGTTGGATTGTTCATTGT+TGG - chr4.2:3196410-3196429 None:intergenic 45.0%
GCTTCTTGCATGTAGAACTG+AGG - chr4.2:3196470-3196489 None:intergenic 45.0%
GTTGGATTTGTACGATGAGG+TGG - chr4.2:3196368-3196387 None:intergenic 45.0%
TGATTAACCCTACCACGACT+CGG - chr4.2:3197268-3197287 None:intergenic 45.0%
TGGTTGAAGATCCGGATGTT+GGG + chr4.2:3197428-3197447 MS.gene06160:CDS 45.0%
TTGTTCATTGTTGGCCATGC+TGG - chr4.2:3196401-3196420 None:intergenic 45.0%
! CAACTGGTTCCGTCTTTACA+CGG - chr4.2:3196833-3196852 None:intergenic 45.0%
! GAACGGAAGAGTTTTCACAC+AGG - chr4.2:3196984-3197003 None:intergenic 45.0%
! GAGTGTCCTTTTTCTCATCC+CGG + chr4.2:3196653-3196672 MS.gene06160:CDS 45.0%
! TAATGGATCGGGTTCAGGTT+CGG + chr4.2:3197399-3197418 MS.gene06160:CDS 45.0%
! TTTTCCGAGTCATGAATCGG+TGG + chr4.2:3196550-3196569 MS.gene06160:CDS 45.0%
!! CACTGCGTTGATTCGTATGA+CGG + chr4.2:3196929-3196948 MS.gene06160:CDS 45.0%
!! GAATCCACCGATTCATGACT+CGG - chr4.2:3196557-3196576 None:intergenic 45.0%
!!! TTGTTTTTTCGCTCACACGG+CGG + chr4.2:3196865-3196884 MS.gene06160:CDS 45.0%
AATCAACGCAGTGCACACTC+CGG - chr4.2:3196922-3196941 None:intergenic 50.0%
AGAACCCACAGATCTTCCCA+AGG - chr4.2:3197077-3197096 None:intergenic 50.0%
CGTACTCAGCCGTGTAAAGA+CGG + chr4.2:3196821-3196840 MS.gene06160:CDS 50.0%
CGTCTTTACACGGCTGAGTA+CGG - chr4.2:3196823-3196842 None:intergenic 50.0%
CTCCGATTACTCCGATGACT+CGG + chr4.2:3197050-3197069 MS.gene06160:CDS 50.0%
CTGAGGATAATCGTCGTTGG+AGG - chr4.2:3196453-3196472 None:intergenic 50.0%
GACCGAGTCATCGGAGTAAT+CGG - chr4.2:3197055-3197074 None:intergenic 50.0%
GACTCAATAGCAAGCCTGAG+AGG - chr4.2:3196959-3196978 None:intergenic 50.0%
GAGCTTGTGAGCCCTTTCTT+GGG + chr4.2:3197460-3197479 MS.gene06160:CDS 50.0%
GATCCAGGAGGAGACATGAA+CGG - chr4.2:3197001-3197020 None:intergenic 50.0%
GATTCGTGTGAGTTTGCGCA+TGG + chr4.2:3196764-3196783 MS.gene06160:CDS 50.0%
GGGTGAAGTGGTTGAAGATC+CGG + chr4.2:3197420-3197439 MS.gene06160:CDS 50.0%
GGTGAAAAAATCTCCGCCGT+TGG - chr4.2:3196425-3196444 None:intergenic 50.0%
GTGGTTGAAGATCCGGATGT+TGG + chr4.2:3197427-3197446 MS.gene06160:CDS 50.0%
GTTCATGTCTCCTCCTGGAT+CGG + chr4.2:3197000-3197019 MS.gene06160:CDS 50.0%
TATACTTTCTCGGGTCACGG+CGG - chr4.2:3196691-3196710 None:intergenic 50.0%
TGAAGATCCGGATGTTGGGT+GGG + chr4.2:3197432-3197451 MS.gene06160:CDS 50.0%
TTCTCGCCGGGATGAGAAAA+AGG - chr4.2:3196662-3196681 None:intergenic 50.0%
TTGAAGATCCGGATGTTGGG+TGG + chr4.2:3197431-3197450 MS.gene06160:CDS 50.0%
! AATGGATCGGGTTCAGGTTC+GGG + chr4.2:3197400-3197419 MS.gene06160:CDS 50.0%
! CGTTGGTAGGCTACAGAATC+CGG - chr4.2:3197230-3197249 None:intergenic 50.0%
! GTTGGTAGGCTACAGAATCC+GGG - chr4.2:3197229-3197248 None:intergenic 50.0%
!! TCTTTGGGATCAGTGTTGCG+AGG + chr4.2:3197294-3197313 MS.gene06160:CDS 50.0%
AAGACGGAACCAGTTGTCGC+CGG + chr4.2:3196837-3196856 MS.gene06160:CDS 55.0%
ACAGAATCCGGGTCGGATCA+CGG - chr4.2:3197218-3197237 None:intergenic 55.0%
ATCAACGCAGTGCACACTCC+GGG - chr4.2:3196921-3196940 None:intergenic 55.0%
CAACCGTACACGTTCCAGCA+TGG + chr4.2:3196384-3196403 MS.gene06160:CDS 55.0%
CATGCTGGAACGTGTACGGT+TGG - chr4.2:3196386-3196405 None:intergenic 55.0%
CGGTCCTTGGGAAGATCTGT+GGG + chr4.2:3197070-3197089 MS.gene06160:CDS 55.0%
CGTATGACGGTTCTCCTCTC+AGG + chr4.2:3196942-3196961 MS.gene06160:CDS 55.0%
CTTCCCAAGGACCGAGTCAT+CGG - chr4.2:3197064-3197083 None:intergenic 55.0%
CTTCCGTTCATGTCTCCTCC+TGG + chr4.2:3196995-3197014 MS.gene06160:CDS 55.0%
GGAGCTTGTGAGCCCTTTCT+TGG + chr4.2:3197459-3197478 MS.gene06160:CDS 55.0%
GGAGTAATCGGAGACATCGG+AGG - chr4.2:3197043-3197062 None:intergenic 55.0%
GGCTGAGTACGGTAACGAGA+AGG - chr4.2:3196812-3196831 None:intergenic 55.0%
GTAGGCTACAGAATCCGGGT+CGG - chr4.2:3197225-3197244 None:intergenic 55.0%
TCACACGGCGGAGCAACTTA+GGG + chr4.2:3196877-3196896 MS.gene06160:CDS 55.0%
TCCGAAACCCACCCAACATC+CGG - chr4.2:3197442-3197461 None:intergenic 55.0%
TCGGTCCTTGGGAAGATCTG+TGG + chr4.2:3197069-3197088 MS.gene06160:CDS 55.0%
TGGCAGGTCACATGACTGGA+CGG + chr4.2:3196631-3196650 MS.gene06160:CDS 55.0%
TGGCCATGCTGGAACGTGTA+CGG - chr4.2:3196390-3196409 None:intergenic 55.0%
TTGCGAGGAAGAGCCTGTGA+TGG + chr4.2:3197309-3197328 MS.gene06160:CDS 55.0%
! AGGAAGAGCCTGTGATGGAG+AGG + chr4.2:3197314-3197333 MS.gene06160:CDS 55.0%
! GGAAGAGCCTGTGATGGAGA+GGG + chr4.2:3197315-3197334 MS.gene06160:CDS 55.0%
! TCCGGATGTTGGGTGGGTTT+CGG + chr4.2:3197438-3197457 MS.gene06160:CDS 55.0%
! TCCTCCTGGATCGGTTTCTC+CGG + chr4.2:3197009-3197028 MS.gene06160:CDS 55.0%
!! ACTCCGATGACTCGGTCCTT+GGG + chr4.2:3197058-3197077 MS.gene06160:CDS 55.0%
!! TACTCCGATGACTCGGTCCT+TGG + chr4.2:3197057-3197076 MS.gene06160:CDS 55.0%
AAGATTCGGCGATGCGCACG+TGG + chr4.2:3196611-3196630 MS.gene06160:CDS 60.0%
ACAAACTGGCCGGCGACAAC+TGG - chr4.2:3196849-3196868 None:intergenic 60.0%
ACCGGAGAAACCGATCCAGG+AGG - chr4.2:3197013-3197032 None:intergenic 60.0%
CACGTGGCAGGTCACATGAC+TGG + chr4.2:3196627-3196646 MS.gene06160:CDS 60.0%
CAGAATCCGGGTCGGATCAC+GGG - chr4.2:3197217-3197236 None:intergenic 60.0%
CCTAGATTCGGGTCTCCCAG+AGG + chr4.2:3197190-3197209 MS.gene06160:CDS 60.0%
CGCATGGTGTGTTCGAGTGC+TGG + chr4.2:3196780-3196799 MS.gene06160:CDS 60.0%
CTCACACGGCGGAGCAACTT+AGG + chr4.2:3196876-3196895 MS.gene06160:CDS 60.0%
CTCCGACTCAACAGAGTCCC+CGG + chr4.2:3196900-3196919 MS.gene06160:CDS 60.0%
CTCCGGGGACTCTGTTGAGT+CGG - chr4.2:3196905-3196924 None:intergenic 60.0%
GACATCGGAGGAGACTCCAC+CGG - chr4.2:3197031-3197050 None:intergenic 60.0%
GACTCCACCCTCTCCATCAC+AGG - chr4.2:3197325-3197344 None:intergenic 60.0%
TCAACGCAGTGCACACTCCG+GGG - chr4.2:3196920-3196939 None:intergenic 60.0%
TCCACCGGAGAAACCGATCC+AGG - chr4.2:3197016-3197035 None:intergenic 60.0%
! AGAGCCTGTGATGGAGAGGG+TGG + chr4.2:3197318-3197337 MS.gene06160:CDS 60.0%
! CCTCTGGGAGACCCGAATCT+AGG - chr4.2:3197193-3197212 None:intergenic 60.0%
! GGGTTCAGGTTCGGGTGAAG+TGG + chr4.2:3197408-3197427 MS.gene06160:CDS 60.0%
! GTGATGGAGAGGGTGGAGTC+TGG + chr4.2:3197325-3197344 MS.gene06160:CDS 60.0%
! TCCTGGATCGGTTTCTCCGG+TGG + chr4.2:3197012-3197031 MS.gene06160:CDS 60.0%
!! CGACGACACCGATTCCGACT+CGG + chr4.2:3196523-3196542 MS.gene06160:CDS 60.0%
AGAGGACCCGTGATCCGACC+CGG + chr4.2:3197208-3197227 MS.gene06160:CDS 65.0%
CCTACCAACGACGCCTACGC+AGG + chr4.2:3197240-3197259 MS.gene06160:CDS 65.0%
GGTCGGATCACGGGTCCTCT+GGG - chr4.2:3197208-3197227 None:intergenic 65.0%
! CCTGCGTAGGCGTCGTTGGT+AGG - chr4.2:3197243-3197262 None:intergenic 65.0%
!! GCAGGTGCCGAGTCGTGGTA+GGG + chr4.2:3197258-3197277 MS.gene06160:CDS 65.0%
ACGGCGGCGAGCTTTCTCGC+CGG - chr4.2:3196675-3196694 None:intergenic 70.0%
GGGTCGGATCACGGGTCCTC+TGG - chr4.2:3197209-3197228 None:intergenic 70.0%
TTCGGCGATGCGCACGTGGC+AGG + chr4.2:3196615-3196634 MS.gene06160:CDS 70.0%
! CCACGACTCGGCACCTGCGT+AGG - chr4.2:3197256-3197275 None:intergenic 70.0%
! GGCACCTGCGTAGGCGTCGT+TGG - chr4.2:3197247-3197266 None:intergenic 70.0%
!! CCTACGCAGGTGCCGAGTCG+TGG + chr4.2:3197253-3197272 MS.gene06160:CDS 70.0%
!! CGCAGGTGCCGAGTCGTGGT+AGG + chr4.2:3197257-3197276 MS.gene06160:CDS 70.0%
CGGCGGCGAGCTTTCTCGCC+GGG - chr4.2:3196674-3196693 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 3196350 3197489 3196350 ID=MS.gene06160
chr4.2 mRNA 3196350 3197489 3196350 ID=MS.gene06160.t1;Parent=MS.gene06160
chr4.2 exon 3196350 3197489 3196350 ID=MS.gene06160.t1.exon1;Parent=MS.gene06160.t1
chr4.2 CDS 3196350 3197489 3196350 ID=cds.MS.gene06160.t1;Parent=MS.gene06160.t1
Gene Sequence

>MS.gene06160

ATGATGTTAGGGGAACCACCTCATCGTACAAATCCAACCGTACACGTTCCAGCATGGCCAACAATGAACAATCCAACGGCGGAGATTTTTTCACCTTTAACCTCCAACGACGATTATCCTCAGTTCTACATGCAAGAAGCACTAAGCGCTTTCCAACACTATGTTAACGAAAACGACGACACCGATTCCGACTCGGAGATTTTTCCGAGTCATGAATCGGTGGATTCGTATTCTAACGATCATTTCCGCATGTTCGAGTTTAAGATTCGGCGATGCGCACGTGGCAGGTCACATGACTGGACGGAGTGTCCTTTTTCTCATCCCGGCGAGAAAGCTCGCCGCCGTGACCCGAGAAAGTATAACTACTCCGGCACTTCGTGTCCTGATTTTCGTAAAGGAAGTTGCAAGAAAGGTGATTCGTGTGAGTTTGCGCATGGTGTGTTCGAGTGCTGGCTTCATCCTTCTCGTTACCGTACTCAGCCGTGTAAAGACGGAACCAGTTGTCGCCGGCCAGTTTGTTTTTTCGCTCACACGGCGGAGCAACTTAGGGCTCCGACTCAACAGAGTCCCCGGAGTGTGCACTGCGTTGATTCGTATGACGGTTCTCCTCTCAGGCTTGCTATTGAGTCTTCCTGTGTGAAAACTCTTCCGTTCATGTCTCCTCCTGGATCGGTTTCTCCGGTGGAGTCTCCTCCGATGTCTCCGATTACTCCGATGACTCGGTCCTTGGGAAGATCTGTGGGTTCTAGCTCTGTGAATGAAATGGTGGCTTCTCTGAGAAACTTACAACTTGGTACCATGAAGTCATTACCTTCTTCATGGAATGTTCAAATGGGTTCTCCTAGATTCGGGTCTCCCAGAGGACCCGTGATCCGACCCGGATTCTGTAGCCTACCAACGACGCCTACGCAGGTGCCGAGTCGTGGTAGGGTTAATCATTTTGATCTTTGGGATCAGTGTTGCGAGGAAGAGCCTGTGATGGAGAGGGTGGAGTCTGGAAGAGATATAAGGGTGAAGATGTTTGAGAAACTGAGCAAAGAGAACTCTTTTAATGGATCGGGTTCAGGTTCGGGTGAAGTGGTTGAAGATCCGGATGTTGGGTGGGTTTCGGAGCTTGTGAGCCCTTTCTTGGGTGATTGA
Protein sequence

>MS.gene06160.t1

MMLGEPPHRTNPTVHVPAWPTMNNPTAEIFSPLTSNDDYPQFYMQEALSAFQHYVNENDDTDSDSEIFPSHESVDSYSNDHFRMFEFKIRRCARGRSHDWTECPFSHPGEKARRRDPRKYNYSGTSCPDFRKGSCKKGDSCEFAHGVFECWLHPSRYRTQPCKDGTSCRRPVCFFAHTAEQLRAPTQQSPRSVHCVDSYDGSPLRLAIESSCVKTLPFMSPPGSVSPVESPPMSPITPMTRSLGRSVGSSSVNEMVASLRNLQLGTMKSLPSSWNVQMGSPRFGSPRGPVIRPGFCSLPTTPTQVPSRGRVNHFDLWDQCCEEEPVMERVESGRDIRVKMFEKLSKENSFNGSGSGSGEVVEDPDVGWVSELVSPFLGD