Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061823.t1 | XP_003625416.1 | 95 | 140 | 5 | 1 | 1 | 138 | 1 | 140 | 4.10E-65 | 257.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061823.t1 | F4JZT3 | 59.2 | 120 | 48 | 1 | 13 | 131 | 11 | 130 | 2.7e-33 | 142.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061823.t1 | G7KYH3 | 95.0 | 140 | 5 | 1 | 1 | 138 | 1 | 140 | 2.9e-65 | 257.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene061823.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061823.t1 | MTR_7g098910 | 95.000 | 140 | 5 | 1 | 1 | 138 | 1 | 140 | 1.59e-92 | 264 |
| MS.gene061823.t1 | MTR_7g099500 | 60.317 | 126 | 49 | 1 | 13 | 137 | 4 | 129 | 4.85e-47 | 148 |
| MS.gene061823.t1 | MTR_8g063580 | 58.594 | 128 | 50 | 1 | 14 | 138 | 6 | 133 | 1.66e-43 | 140 |
| MS.gene061823.t1 | MTR_0022s0450 | 77.049 | 61 | 14 | 0 | 26 | 86 | 40 | 100 | 1.45e-26 | 95.9 |
| MS.gene061823.t1 | MTR_7g098860 | 46.392 | 97 | 47 | 3 | 33 | 124 | 2 | 98 | 9.99e-17 | 70.9 |
| MS.gene061823.t1 | MTR_6g008860 | 32.743 | 113 | 75 | 1 | 12 | 123 | 25 | 137 | 3.24e-16 | 70.9 |
| MS.gene061823.t1 | MTR_4g071650 | 32.743 | 113 | 75 | 1 | 12 | 123 | 25 | 137 | 3.24e-16 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061823.t1 | AT5G26594 | 59.167 | 120 | 48 | 1 | 13 | 131 | 11 | 130 | 2.12e-45 | 145 |
| MS.gene061823.t1 | AT3G04280 | 47.934 | 121 | 56 | 3 | 20 | 133 | 22 | 142 | 6.20e-29 | 103 |
| MS.gene061823.t1 | AT3G04280 | 47.934 | 121 | 56 | 3 | 20 | 133 | 22 | 142 | 6.20e-29 | 103 |
| MS.gene061823.t1 | AT3G04280 | 47.934 | 121 | 56 | 3 | 20 | 133 | 22 | 142 | 6.20e-29 | 103 |
Find 28 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAATGGAACCTCAAAATTC+AGG | 0.370359 | 7.3:+13383437 | MS.gene061823:CDS |
| TAACATCATTCTCATGGATA+AGG | 0.382325 | 7.3:+13383604 | MS.gene061823:CDS |
| AGGGAAAGGGAAAGGGATAA+TGG | 0.389184 | 7.3:+13383421 | MS.gene061823:CDS |
| ACCCATCGATCGCAGTTTCT+TGG | 0.391435 | 7.3:-13383834 | None:intergenic |
| AAACAAGAATTTATGGAAGC+AGG | 0.424926 | 7.3:+13383902 | MS.gene061823:CDS |
| AAGGAAATGCCTATCATGAA+TGG | 0.455972 | 7.3:+13383623 | MS.gene061823:CDS |
| AAATGGAAGATCAAGGGAAA+GGG | 0.458192 | 7.3:+13383408 | None:intergenic |
| AAGATTTAACATCATTCTCA+TGG | 0.473978 | 7.3:+13383598 | MS.gene061823:CDS |
| ACCTCAATCCCATTCATGAT+AGG | 0.485829 | 7.3:-13383632 | None:intergenic |
| TTAAGCACACCAATGGTCAA+TGG | 0.495806 | 7.3:-13383947 | None:intergenic |
| TTGTACAAAATGGAAGATCA+AGG | 0.500428 | 7.3:+13383401 | None:intergenic |
| AAGATCAAGGGAAAGGGAAA+GGG | 0.503589 | 7.3:+13383414 | MS.gene061823:CDS |
| AGGAAATGCCTATCATGAAT+GGG | 0.507526 | 7.3:+13383624 | MS.gene061823:CDS |
| TAAATCTTTGACCATTGCAA+TGG | 0.518192 | 7.3:-13383583 | None:intergenic |
| AACAAGAATTTATGGAAGCA+GGG | 0.519897 | 7.3:+13383903 | MS.gene061823:CDS |
| GCCTATCATGAATGGGATTG+AGG | 0.520152 | 7.3:+13383631 | MS.gene061823:CDS |
| GAAGATCAAGGGAAAGGGAA+AGG | 0.522711 | 7.3:+13383413 | MS.gene061823:CDS |
| AACCAAGAAACTGCGATCGA+TGG | 0.533042 | 7.3:+13383832 | MS.gene061823:CDS |
| CTTGTTCGAATGATTCATCA+AGG | 0.541897 | 7.3:+13383497 | MS.gene061823:CDS |
| CAAGGTCTATTGAAAAGAGC+TGG | 0.545224 | 7.3:+13383515 | MS.gene061823:CDS |
| ACCAAGAAACTGCGATCGAT+GGG | 0.549417 | 7.3:+13383833 | MS.gene061823:CDS |
| CAAGTGAAACCATTGACCAT+TGG | 0.555088 | 7.3:+13383938 | MS.gene061823:CDS |
| GGTATCCGTAGCATGATTGT+TGG | 0.563539 | 7.3:+13383854 | MS.gene061823:CDS |
| AAAATGGAAGATCAAGGGAA+AGG | 0.568166 | 7.3:+13383407 | None:intergenic |
| ACACACCAACAATCATGCTA+CGG | 0.608934 | 7.3:-13383859 | None:intergenic |
| TGTACAAAATGGAAGATCAA+GGG | 0.616219 | 7.3:+13383402 | None:intergenic |
| AATCGAATTAAGCACACCAA+TGG | 0.617947 | 7.3:-13383954 | None:intergenic |
| GCTGTAGATATCCATTGCAA+TGG | 0.620859 | 7.3:+13383572 | MS.gene061823:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATACATAACAAAGTTAAT+AGG | - | chr7.3:13383726-13383745 | None:intergenic | 15.0% |
| !!! | ATTTTAAATATAATTGCACA+TGG | - | chr7.3:13383779-13383798 | None:intergenic | 15.0% |
| ! | AAAAGTGAAGCTGTAAAAAA+TGG | + | chr7.3:13383542-13383561 | MS.gene061823:CDS | 25.0% |
| ! | AAGATTTAACATCATTCTCA+TGG | + | chr7.3:13383598-13383617 | MS.gene061823:CDS | 25.0% |
| ! | AGCAGAAAAACAAGAATTTA+TGG | + | chr7.3:13383895-13383914 | MS.gene061823:CDS | 25.0% |
| ! | TTAAAATGAGATTCACTTGT+TGG | + | chr7.3:13383792-13383811 | MS.gene061823:intron | 25.0% |
| !!! | TTGTTGGATTTTTTTAATGC+AGG | + | chr7.3:13383808-13383827 | MS.gene061823:intron | 25.0% |
| AAACAAGAATTTATGGAAGC+AGG | + | chr7.3:13383902-13383921 | MS.gene061823:CDS | 30.0% | |
| AACAAGAATTTATGGAAGCA+GGG | + | chr7.3:13383903-13383922 | MS.gene061823:CDS | 30.0% | |
| TAAATCTTTGACCATTGCAA+TGG | - | chr7.3:13383586-13383605 | None:intergenic | 30.0% | |
| TAACATCATTCTCATGGATA+AGG | + | chr7.3:13383604-13383623 | MS.gene061823:CDS | 30.0% | |
| ! | AATTCAGTTCCTGAATTTTG+AGG | - | chr7.3:13383449-13383468 | None:intergenic | 30.0% |
| ! | ATAATGGAACCTCAAAATTC+AGG | + | chr7.3:13383437-13383456 | MS.gene061823:CDS | 30.0% |
| AAGGAAATGCCTATCATGAA+TGG | + | chr7.3:13383623-13383642 | MS.gene061823:CDS | 35.0% | |
| AATCGAATTAAGCACACCAA+TGG | - | chr7.3:13383957-13383976 | None:intergenic | 35.0% | |
| AGGAAATGCCTATCATGAAT+GGG | + | chr7.3:13383624-13383643 | MS.gene061823:CDS | 35.0% | |
| CTTGTTCGAATGATTCATCA+AGG | + | chr7.3:13383497-13383516 | MS.gene061823:CDS | 35.0% | |
| AAGATCAAGGGAAAGGGAAA+GGG | + | chr7.3:13383414-13383433 | MS.gene061823:CDS | 40.0% | |
| ACACACCAACAATCATGCTA+CGG | - | chr7.3:13383862-13383881 | None:intergenic | 40.0% | |
| ACCTCAATCCCATTCATGAT+AGG | - | chr7.3:13383635-13383654 | None:intergenic | 40.0% | |
| CAAGGTCTATTGAAAAGAGC+TGG | + | chr7.3:13383515-13383534 | MS.gene061823:CDS | 40.0% | |
| CAAGTGAAACCATTGACCAT+TGG | + | chr7.3:13383938-13383957 | MS.gene061823:CDS | 40.0% | |
| GCTGTAGATATCCATTGCAA+TGG | + | chr7.3:13383572-13383591 | MS.gene061823:CDS | 40.0% | |
| TTAAGCACACCAATGGTCAA+TGG | - | chr7.3:13383950-13383969 | None:intergenic | 40.0% | |
| AACCAAGAAACTGCGATCGA+TGG | + | chr7.3:13383832-13383851 | MS.gene061823:CDS | 45.0% | |
| ACCAAGAAACTGCGATCGAT+GGG | + | chr7.3:13383833-13383852 | MS.gene061823:CDS | 45.0% | |
| AGGGAAAGGGAAAGGGATAA+TGG | + | chr7.3:13383421-13383440 | MS.gene061823:CDS | 45.0% | |
| GAAGATCAAGGGAAAGGGAA+AGG | + | chr7.3:13383413-13383432 | MS.gene061823:CDS | 45.0% | |
| GCCTATCATGAATGGGATTG+AGG | + | chr7.3:13383631-13383650 | MS.gene061823:CDS | 45.0% | |
| GGTATCCGTAGCATGATTGT+TGG | + | chr7.3:13383854-13383873 | MS.gene061823:CDS | 45.0% | |
| ACCCATCGATCGCAGTTTCT+TGG | - | chr7.3:13383837-13383856 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 13383410 | 13384003 | 13383410 | ID=MS.gene061823 |
| chr7.3 | mRNA | 13383410 | 13384003 | 13383410 | ID=MS.gene061823.t1;Parent=MS.gene061823 |
| chr7.3 | exon | 13383410 | 13383652 | 13383410 | ID=MS.gene061823.t1.exon1;Parent=MS.gene061823.t1 |
| chr7.3 | CDS | 13383410 | 13383652 | 13383410 | ID=cds.MS.gene061823.t1;Parent=MS.gene061823.t1 |
| chr7.3 | exon | 13383830 | 13384003 | 13383830 | ID=MS.gene061823.t1.exon2;Parent=MS.gene061823.t1 |
| chr7.3 | CDS | 13383830 | 13384003 | 13383830 | ID=cds.MS.gene061823.t1;Parent=MS.gene061823.t1 |
| Gene Sequence |
| Protein sequence |
