Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061825.t1 | XP_003625416.1 | 95 | 140 | 5 | 1 | 1 | 138 | 1 | 140 | 4.10E-65 | 257.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061825.t1 | F4JZT3 | 59.2 | 120 | 48 | 1 | 13 | 131 | 11 | 130 | 2.7e-33 | 142.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061825.t1 | G7KYH3 | 95.0 | 140 | 5 | 1 | 1 | 138 | 1 | 140 | 2.9e-65 | 257.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene061825.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061825.t1 | MTR_7g098910 | 95.000 | 140 | 5 | 1 | 1 | 138 | 1 | 140 | 1.59e-92 | 264 |
| MS.gene061825.t1 | MTR_7g099500 | 60.317 | 126 | 49 | 1 | 13 | 137 | 4 | 129 | 4.85e-47 | 148 |
| MS.gene061825.t1 | MTR_8g063580 | 58.594 | 128 | 50 | 1 | 14 | 138 | 6 | 133 | 1.66e-43 | 140 |
| MS.gene061825.t1 | MTR_0022s0450 | 77.049 | 61 | 14 | 0 | 26 | 86 | 40 | 100 | 1.45e-26 | 95.9 |
| MS.gene061825.t1 | MTR_7g098860 | 46.392 | 97 | 47 | 3 | 33 | 124 | 2 | 98 | 9.99e-17 | 70.9 |
| MS.gene061825.t1 | MTR_6g008860 | 32.743 | 113 | 75 | 1 | 12 | 123 | 25 | 137 | 3.24e-16 | 70.9 |
| MS.gene061825.t1 | MTR_4g071650 | 32.743 | 113 | 75 | 1 | 12 | 123 | 25 | 137 | 3.24e-16 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061825.t1 | AT5G26594 | 59.167 | 120 | 48 | 1 | 13 | 131 | 11 | 130 | 2.12e-45 | 145 |
| MS.gene061825.t1 | AT3G04280 | 47.934 | 121 | 56 | 3 | 20 | 133 | 22 | 142 | 6.20e-29 | 103 |
| MS.gene061825.t1 | AT3G04280 | 47.934 | 121 | 56 | 3 | 20 | 133 | 22 | 142 | 6.20e-29 | 103 |
| MS.gene061825.t1 | AT3G04280 | 47.934 | 121 | 56 | 3 | 20 | 133 | 22 | 142 | 6.20e-29 | 103 |
Find 28 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAATGGAACCTCAAAATTC+AGG | 0.370359 | 7.4:+11556262 | MS.gene061825:CDS |
| TAACATCATTCTCATGGATA+AGG | 0.382325 | 7.4:+11556429 | MS.gene061825:CDS |
| AGGGAAAGGGAAAGGGATAA+TGG | 0.389184 | 7.4:+11556246 | MS.gene061825:CDS |
| ACCCATCGATCGCAGTTTCT+TGG | 0.391435 | 7.4:-11556659 | None:intergenic |
| AAACAAGAATTTATGGAAGC+AGG | 0.424926 | 7.4:+11556727 | MS.gene061825:CDS |
| AAGGAAATGCCTATCATGAA+TGG | 0.455972 | 7.4:+11556448 | MS.gene061825:CDS |
| AAATGGAAGATCAAGGGAAA+GGG | 0.458192 | 7.4:+11556233 | None:intergenic |
| AAGATTTAACATCATTCTCA+TGG | 0.473978 | 7.4:+11556423 | MS.gene061825:CDS |
| ACCTCAATCCCATTCATGAT+AGG | 0.485829 | 7.4:-11556457 | None:intergenic |
| TTAAGCACACCAATGGTCAA+TGG | 0.495806 | 7.4:-11556772 | None:intergenic |
| TTGTACAAAATGGAAGATCA+AGG | 0.500428 | 7.4:+11556226 | None:intergenic |
| AAGATCAAGGGAAAGGGAAA+GGG | 0.503589 | 7.4:+11556239 | MS.gene061825:CDS |
| AGGAAATGCCTATCATGAAT+GGG | 0.507526 | 7.4:+11556449 | MS.gene061825:CDS |
| TAAATCTTTGACCATTGCAA+TGG | 0.518192 | 7.4:-11556408 | None:intergenic |
| AACAAGAATTTATGGAAGCA+GGG | 0.519897 | 7.4:+11556728 | MS.gene061825:CDS |
| GCCTATCATGAATGGGATTG+AGG | 0.520152 | 7.4:+11556456 | MS.gene061825:CDS |
| GAAGATCAAGGGAAAGGGAA+AGG | 0.522711 | 7.4:+11556238 | MS.gene061825:CDS |
| AACCAAGAAACTGCGATCGA+TGG | 0.533042 | 7.4:+11556657 | MS.gene061825:CDS |
| CTTGTTCGAATGATTCATCA+AGG | 0.541897 | 7.4:+11556322 | MS.gene061825:CDS |
| CAAGGTCTATTGAAAAGAGC+TGG | 0.545224 | 7.4:+11556340 | MS.gene061825:CDS |
| ACCAAGAAACTGCGATCGAT+GGG | 0.549417 | 7.4:+11556658 | MS.gene061825:CDS |
| CAAGTGAAACCATTGACCAT+TGG | 0.555088 | 7.4:+11556763 | MS.gene061825:CDS |
| GGTATCCGTAGCATGATTGT+TGG | 0.563539 | 7.4:+11556679 | MS.gene061825:CDS |
| AAAATGGAAGATCAAGGGAA+AGG | 0.568166 | 7.4:+11556232 | None:intergenic |
| ACACACCAACAATCATGCTA+CGG | 0.608934 | 7.4:-11556684 | None:intergenic |
| TGTACAAAATGGAAGATCAA+GGG | 0.616219 | 7.4:+11556227 | None:intergenic |
| AATCGAATTAAGCACACCAA+TGG | 0.617947 | 7.4:-11556779 | None:intergenic |
| GCTGTAGATATCCATTGCAA+TGG | 0.620859 | 7.4:+11556397 | MS.gene061825:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATACATAACAAAGTTAAT+AGG | - | chr7.4:11556551-11556570 | None:intergenic | 15.0% |
| !!! | ATTTTAAATATAATTGCACA+TGG | - | chr7.4:11556604-11556623 | None:intergenic | 15.0% |
| ! | AAAAGTGAAGCTGTAAAAAA+TGG | + | chr7.4:11556367-11556386 | MS.gene061825:CDS | 25.0% |
| ! | AAGATTTAACATCATTCTCA+TGG | + | chr7.4:11556423-11556442 | MS.gene061825:CDS | 25.0% |
| ! | AGCAGAAAAACAAGAATTTA+TGG | + | chr7.4:11556720-11556739 | MS.gene061825:CDS | 25.0% |
| ! | TTAAAATGAGATTCACTTGT+TGG | + | chr7.4:11556617-11556636 | MS.gene061825:intron | 25.0% |
| !!! | TTGTTGGATTTTTTTAATGC+AGG | + | chr7.4:11556633-11556652 | MS.gene061825:intron | 25.0% |
| AAACAAGAATTTATGGAAGC+AGG | + | chr7.4:11556727-11556746 | MS.gene061825:CDS | 30.0% | |
| AACAAGAATTTATGGAAGCA+GGG | + | chr7.4:11556728-11556747 | MS.gene061825:CDS | 30.0% | |
| TAAATCTTTGACCATTGCAA+TGG | - | chr7.4:11556411-11556430 | None:intergenic | 30.0% | |
| TAACATCATTCTCATGGATA+AGG | + | chr7.4:11556429-11556448 | MS.gene061825:CDS | 30.0% | |
| ! | AATTCAGTTCCTGAATTTTG+AGG | - | chr7.4:11556274-11556293 | None:intergenic | 30.0% |
| ! | ATAATGGAACCTCAAAATTC+AGG | + | chr7.4:11556262-11556281 | MS.gene061825:CDS | 30.0% |
| AAGGAAATGCCTATCATGAA+TGG | + | chr7.4:11556448-11556467 | MS.gene061825:CDS | 35.0% | |
| AATCGAATTAAGCACACCAA+TGG | - | chr7.4:11556782-11556801 | None:intergenic | 35.0% | |
| AGGAAATGCCTATCATGAAT+GGG | + | chr7.4:11556449-11556468 | MS.gene061825:CDS | 35.0% | |
| CTTGTTCGAATGATTCATCA+AGG | + | chr7.4:11556322-11556341 | MS.gene061825:CDS | 35.0% | |
| AAGATCAAGGGAAAGGGAAA+GGG | + | chr7.4:11556239-11556258 | MS.gene061825:CDS | 40.0% | |
| ACACACCAACAATCATGCTA+CGG | - | chr7.4:11556687-11556706 | None:intergenic | 40.0% | |
| ACCTCAATCCCATTCATGAT+AGG | - | chr7.4:11556460-11556479 | None:intergenic | 40.0% | |
| CAAGGTCTATTGAAAAGAGC+TGG | + | chr7.4:11556340-11556359 | MS.gene061825:CDS | 40.0% | |
| CAAGTGAAACCATTGACCAT+TGG | + | chr7.4:11556763-11556782 | MS.gene061825:CDS | 40.0% | |
| GCTGTAGATATCCATTGCAA+TGG | + | chr7.4:11556397-11556416 | MS.gene061825:CDS | 40.0% | |
| TTAAGCACACCAATGGTCAA+TGG | - | chr7.4:11556775-11556794 | None:intergenic | 40.0% | |
| AACCAAGAAACTGCGATCGA+TGG | + | chr7.4:11556657-11556676 | MS.gene061825:CDS | 45.0% | |
| ACCAAGAAACTGCGATCGAT+GGG | + | chr7.4:11556658-11556677 | MS.gene061825:CDS | 45.0% | |
| AGGGAAAGGGAAAGGGATAA+TGG | + | chr7.4:11556246-11556265 | MS.gene061825:CDS | 45.0% | |
| GAAGATCAAGGGAAAGGGAA+AGG | + | chr7.4:11556238-11556257 | MS.gene061825:CDS | 45.0% | |
| GCCTATCATGAATGGGATTG+AGG | + | chr7.4:11556456-11556475 | MS.gene061825:CDS | 45.0% | |
| GGTATCCGTAGCATGATTGT+TGG | + | chr7.4:11556679-11556698 | MS.gene061825:CDS | 45.0% | |
| ACCCATCGATCGCAGTTTCT+TGG | - | chr7.4:11556662-11556681 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 11556235 | 11556828 | 11556235 | ID=MS.gene061825 |
| chr7.4 | mRNA | 11556235 | 11556828 | 11556235 | ID=MS.gene061825.t1;Parent=MS.gene061825 |
| chr7.4 | exon | 11556235 | 11556477 | 11556235 | ID=MS.gene061825.t1.exon1;Parent=MS.gene061825.t1 |
| chr7.4 | CDS | 11556235 | 11556477 | 11556235 | ID=cds.MS.gene061825.t1;Parent=MS.gene061825.t1 |
| chr7.4 | exon | 11556655 | 11556828 | 11556655 | ID=MS.gene061825.t1.exon2;Parent=MS.gene061825.t1 |
| chr7.4 | CDS | 11556655 | 11556828 | 11556655 | ID=cds.MS.gene061825.t1;Parent=MS.gene061825.t1 |
| Gene Sequence |
| Protein sequence |
