Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061861.t1 | XP_003625472.1 | 99.2 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 1.10E-64 | 255.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061861.t1 | F4JZT3 | 63.0 | 119 | 44 | 0 | 9 | 127 | 16 | 134 | 4.6e-35 | 148.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061861.t1 | G7KZK5 | 99.2 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 8.0e-65 | 255.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene061861.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061861.t1 | MTR_7g099500 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 2.67e-93 | 265 |
| MS.gene061861.t1 | MTR_8g063580 | 67.442 | 129 | 39 | 2 | 1 | 126 | 1 | 129 | 1.19e-54 | 168 |
| MS.gene061861.t1 | MTR_7g098910 | 59.200 | 125 | 50 | 1 | 5 | 129 | 16 | 139 | 9.52e-46 | 145 |
| MS.gene061861.t1 | MTR_6g008860 | 34.211 | 114 | 75 | 0 | 11 | 124 | 33 | 146 | 3.15e-18 | 75.9 |
| MS.gene061861.t1 | MTR_4g071650 | 34.211 | 114 | 75 | 0 | 11 | 124 | 33 | 146 | 3.15e-18 | 75.9 |
| MS.gene061861.t1 | MTR_7g098860 | 47.500 | 80 | 41 | 1 | 34 | 112 | 12 | 91 | 1.09e-15 | 67.8 |
| MS.gene061861.t1 | MTR_0022s0450 | 52.459 | 61 | 29 | 0 | 17 | 77 | 40 | 100 | 1.23e-14 | 65.1 |
| MS.gene061861.t1 | MTR_8g106150 | 27.820 | 133 | 79 | 1 | 14 | 129 | 621 | 753 | 1.72e-12 | 63.5 |
| MS.gene061861.t1 | MTR_8g106150 | 27.820 | 133 | 79 | 1 | 14 | 129 | 864 | 996 | 1.76e-12 | 63.5 |
| MS.gene061861.t1 | MTR_5g044100 | 43.284 | 67 | 38 | 0 | 14 | 80 | 155 | 221 | 2.01e-12 | 62.0 |
| MS.gene061861.t1 | MTR_5g097410 | 41.791 | 67 | 39 | 0 | 14 | 80 | 1077 | 1143 | 1.42e-11 | 60.8 |
| MS.gene061861.t1 | MTR_8g075340 | 30.597 | 134 | 72 | 3 | 11 | 123 | 1029 | 1162 | 2.20e-11 | 60.1 |
| MS.gene061861.t1 | MTR_5g022470 | 30.000 | 150 | 74 | 4 | 11 | 129 | 1050 | 1199 | 9.51e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061861.t1 | AT5G26594 | 63.025 | 119 | 44 | 0 | 9 | 127 | 16 | 134 | 1.25e-48 | 152 |
| MS.gene061861.t1 | AT3G04280 | 46.789 | 109 | 56 | 2 | 11 | 117 | 22 | 130 | 6.40e-26 | 95.1 |
| MS.gene061861.t1 | AT3G04280 | 46.789 | 109 | 56 | 2 | 11 | 117 | 22 | 130 | 6.40e-26 | 95.1 |
| MS.gene061861.t1 | AT3G04280 | 46.789 | 109 | 56 | 2 | 11 | 117 | 22 | 130 | 6.40e-26 | 95.1 |
| MS.gene061861.t1 | AT2G01830 | 30.657 | 137 | 82 | 2 | 2 | 125 | 922 | 1058 | 4.46e-13 | 65.1 |
| MS.gene061861.t1 | AT2G01830 | 30.657 | 137 | 82 | 2 | 2 | 125 | 933 | 1069 | 4.87e-13 | 65.1 |
| MS.gene061861.t1 | AT2G01830 | 30.657 | 137 | 82 | 2 | 2 | 125 | 910 | 1046 | 5.10e-13 | 65.1 |
| MS.gene061861.t1 | AT2G01830 | 30.657 | 137 | 82 | 2 | 2 | 125 | 910 | 1046 | 5.10e-13 | 65.1 |
| MS.gene061861.t1 | AT2G01830 | 30.657 | 137 | 82 | 2 | 2 | 125 | 910 | 1046 | 5.10e-13 | 65.1 |
| MS.gene061861.t1 | AT2G01830 | 30.657 | 137 | 82 | 2 | 2 | 125 | 910 | 1046 | 5.10e-13 | 65.1 |
| MS.gene061861.t1 | AT5G35750 | 28.889 | 135 | 74 | 2 | 14 | 126 | 1038 | 1172 | 1.58e-12 | 63.5 |
Find 31 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAAGATGTTGAATAAATT+TGG | 0.144966 | 7.4:-10879607 | MS.gene061861:CDS |
| GCAATACTTAGTTCCATTCT+TGG | 0.228362 | 7.4:-10878258 | MS.gene061861:CDS |
| TATCCATGTCCATGAGAATT+AGG | 0.311699 | 7.4:+10879515 | None:intergenic |
| TGGATTGATCTTGCCAAGAA+TGG | 0.418939 | 7.4:+10878245 | None:intergenic |
| CACAACCAGTGCTGTTAGCT+TGG | 0.421611 | 7.4:+10879660 | None:intergenic |
| AAAATTCATGGAAGCTGGAT+TGG | 0.421768 | 7.4:-10878310 | MS.gene061861:CDS |
| TGGTGTGTCATCACGCTCTA+TGG | 0.423024 | 7.4:-10878346 | MS.gene061861:CDS |
| AGATGAAATACTAAAATTCA+TGG | 0.428022 | 7.4:-10878322 | MS.gene061861:CDS |
| ACTAAGTATTGCATTGTTCA+AGG | 0.440010 | 7.4:+10878269 | None:intergenic |
| AAGAAGCAGTGGACATTCAT+TGG | 0.445266 | 7.4:-10879555 | MS.gene061861:CDS |
| ATACTAAAATTCATGGAAGC+TGG | 0.448559 | 7.4:-10878315 | MS.gene061861:CDS |
| GGTATTTGTAGCACAATTGT+TGG | 0.475026 | 7.4:-10878366 | MS.gene061861:CDS |
| AAAATTTGACCTAATTCTCA+TGG | 0.477878 | 7.4:-10879524 | MS.gene061861:CDS |
| AAAAGTCAAGGTGTGGAGAA+TGG | 0.478580 | 7.4:-10879580 | MS.gene061861:CDS |
| ACAAAGGAACTTCGTGCAAT+GGG | 0.482431 | 7.4:-10878387 | MS.gene061861:CDS |
| CAAGCTAACAGCACTGGTTG+TGG | 0.482562 | 7.4:-10879659 | MS.gene061861:CDS |
| ACCTCAATGCCATTCATGAT+AGG | 0.492567 | 7.4:+10879490 | None:intergenic |
| CCTTGCATCCATCACACAGT+TGG | 0.511788 | 7.4:+10878225 | None:intergenic |
| AACAAAGGAACTTCGTGCAA+TGG | 0.518430 | 7.4:-10878388 | MS.gene061861:CDS |
| ATGGATATGCCTATCATGAA+TGG | 0.542735 | 7.4:-10879499 | MS.gene061861:CDS |
| CTAAGTATTGCATTGTTCAA+GGG | 0.546876 | 7.4:+10878270 | None:intergenic |
| CAGTGGACATTCATTGGCGT+GGG | 0.559565 | 7.4:-10879549 | MS.gene061861:CDS |
| GCCTATCATGAATGGCATTG+AGG | 0.559799 | 7.4:-10879491 | MS.gene061861:intron |
| TGTATCCAAGCTAACAGCAC+TGG | 0.575373 | 7.4:-10879665 | MS.gene061861:CDS |
| TGTGTTTATATAGGCAACAA+AGG | 0.581181 | 7.4:-10878403 | MS.gene061861:intron |
| AGATCAATCCAACTGTGTGA+TGG | 0.583988 | 7.4:-10878233 | None:intergenic |
| TGACCTAATTCTCATGGACA+TGG | 0.592027 | 7.4:-10879518 | MS.gene061861:CDS |
| GCAGTGGACATTCATTGGCG+TGG | 0.597898 | 7.4:-10879550 | MS.gene061861:CDS |
| AAATTTGGAGTCAAAAGTCA+AGG | 0.637368 | 7.4:-10879592 | MS.gene061861:CDS |
| TGGAGTCAAAAGTCAAGGTG+TGG | 0.637883 | 7.4:-10879587 | MS.gene061861:CDS |
| GGAGAATGGAAAAGAAGCAG+TGG | 0.653869 | 7.4:-10879566 | MS.gene061861:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATAAATATAAATAAAAA+AGG | + | chr7.4:10879486-10879505 | None:intergenic | 0.0% |
| !! | ACTAAAATTAACTATAAAAT+TGG | + | chr7.4:10879212-10879231 | None:intergenic | 10.0% |
| !! | CATATAAAAATATATAAACT+TGG | + | chr7.4:10878678-10878697 | None:intergenic | 10.0% |
| !! | TATAAAAATATATAAACTTG+GGG | + | chr7.4:10878676-10878695 | None:intergenic | 10.0% |
| !!! | AAAAAAATATTTTACTTTTG+AGG | + | chr7.4:10879042-10879061 | None:intergenic | 10.0% |
| !!! | AATTTTATAGTTAATTTTAG+TGG | - | chr7.4:10879211-10879230 | MS.gene061861:intron | 10.0% |
| !! | AAAATTAACTATAAAATTGG+AGG | + | chr7.4:10879209-10879228 | None:intergenic | 15.0% |
| !! | AAATTAACTATAAAATTGGA+GGG | + | chr7.4:10879208-10879227 | None:intergenic | 15.0% |
| !!! | ATATTTATATAGTTTGTTAG+AGG | - | chr7.4:10878893-10878912 | MS.gene061861:intron | 15.0% |
| !! | AGATGAAATACTAAAATTCA+TGG | - | chr7.4:10879599-10879618 | MS.gene061861:CDS | 20.0% |
| !! | CAAAAGATGTTGAATAAATT+TGG | - | chr7.4:10878314-10878333 | MS.gene061861:CDS | 20.0% |
| !! | CTCTATAATTAATTAAGTAC+AGG | + | chr7.4:10878460-10878479 | None:intergenic | 20.0% |
| !! | GAAGAAAATGAAAATACTTA+TGG | - | chr7.4:10878771-10878790 | MS.gene061861:intron | 20.0% |
| !! | TGATTATGGTCTATATAATT+TGG | - | chr7.4:10878696-10878715 | MS.gene061861:intron | 20.0% |
| ! | AAAATTTGACCTAATTCTCA+TGG | - | chr7.4:10878397-10878416 | MS.gene061861:intron | 25.0% |
| ! | ACTAAAGGAATTTAAGAATG+AGG | + | chr7.4:10878956-10878975 | None:intergenic | 25.0% |
| ! | GTCAAAAAGTTGTAATAAAG+TGG | - | chr7.4:10878616-10878635 | MS.gene061861:intron | 25.0% |
| ! | TAGTGTACTATTATTGTTCA+TGG | - | chr7.4:10878749-10878768 | MS.gene061861:intron | 25.0% |
| ! | TGATATATGTACAACCATTT+GGG | - | chr7.4:10878483-10878502 | MS.gene061861:intron | 25.0% |
| ! | TGTGTGTTATGTGTTTATAT+AGG | - | chr7.4:10879509-10879528 | MS.gene061861:CDS | 25.0% |
| ! | TTAAATTCCTTTAGTGTTCA+GGG | - | chr7.4:10878961-10878980 | MS.gene061861:intron | 25.0% |
| !! | ACATCTTTTGATGAATCTTT+CGG | + | chr7.4:10878304-10878323 | None:intergenic | 25.0% |
| !! | TTCATTTTCTCTCTTTTCAT+TGG | - | chr7.4:10878523-10878542 | MS.gene061861:intron | 25.0% |
| !!! | CTCATTTGATTTTGTTACTA+TGG | - | chr7.4:10879100-10879119 | MS.gene061861:intron | 25.0% |
| !!! | GATAGTTTTATTGAGAAAGA+AGG | + | chr7.4:10879377-10879396 | None:intergenic | 25.0% |
| !!! | TTATATAGTTTGTTAGAGGA+AGG | - | chr7.4:10878897-10878916 | MS.gene061861:intron | 25.0% |
| AAAGAAGGAATTAGTACAAG+TGG | + | chr7.4:10879362-10879381 | None:intergenic | 30.0% | |
| AGGAAAATATGTTCACTCAT+TGG | - | chr7.4:10879322-10879341 | MS.gene061861:intron | 30.0% | |
| ATACTAAAATTCATGGAAGC+TGG | - | chr7.4:10879606-10879625 | MS.gene061861:CDS | 30.0% | |
| CTTAAATTCCTTTAGTGTTC+AGG | - | chr7.4:10878960-10878979 | MS.gene061861:intron | 30.0% | |
| GTGATATATGTACAACCATT+TGG | - | chr7.4:10878482-10878501 | MS.gene061861:intron | 30.0% | |
| TGAGTGAACTTTATAATACG+AGG | + | chr7.4:10879084-10879103 | None:intergenic | 30.0% | |
| TGGTAACAAATATCGAATGT+TGG | - | chr7.4:10878929-10878948 | MS.gene061861:intron | 30.0% | |
| TGTGTTTATATAGGCAACAA+AGG | - | chr7.4:10879518-10879537 | MS.gene061861:CDS | 30.0% | |
| TTAAGTCTCTCTAGATGTTT+AGG | - | chr7.4:10879254-10879273 | MS.gene061861:intron | 30.0% | |
| TTAGAGGAAGGAGAAATTAT+TGG | - | chr7.4:10878909-10878928 | MS.gene061861:intron | 30.0% | |
| ! | AAATTTGGAGTCAAAAGTCA+AGG | - | chr7.4:10878329-10878348 | MS.gene061861:CDS | 30.0% |
| ! | ACTAAGTATTGCATTGTTCA+AGG | + | chr7.4:10879655-10879674 | None:intergenic | 30.0% |
| ! | CTAAGTATTGCATTGTTCAA+GGG | + | chr7.4:10879654-10879673 | None:intergenic | 30.0% |
| AAAATTCATGGAAGCTGGAT+TGG | - | chr7.4:10879611-10879630 | MS.gene061861:CDS | 35.0% | |
| AAAGTTGTGTCTTTACTTGC+AGG | + | chr7.4:10879000-10879019 | None:intergenic | 35.0% | |
| AAATACTTATGGCCCAACAT+TGG | - | chr7.4:10878782-10878801 | MS.gene061861:intron | 35.0% | |
| AAGTTGTGTCTTTACTTGCA+GGG | + | chr7.4:10878999-10879018 | None:intergenic | 35.0% | |
| ACAACTAAGCTACATCTTCA+AGG | - | chr7.4:10879295-10879314 | MS.gene061861:intron | 35.0% | |
| ATGGATATGCCTATCATGAA+TGG | - | chr7.4:10878422-10878441 | MS.gene061861:intron | 35.0% | |
| CAACTAAGCTACATCTTCAA+GGG | - | chr7.4:10879296-10879315 | MS.gene061861:intron | 35.0% | |
| GAAGATGTAGCTTAGTTGTA+TGG | + | chr7.4:10879294-10879313 | None:intergenic | 35.0% | |
| GCAATACTTAGTTCCATTCT+TGG | - | chr7.4:10879663-10879682 | MS.gene061861:CDS | 35.0% | |
| GGTATTTGTAGCACAATTGT+TGG | - | chr7.4:10879555-10879574 | MS.gene061861:CDS | 35.0% | |
| TATCCATGTCCATGAGAATT+AGG | + | chr7.4:10878409-10878428 | None:intergenic | 35.0% | |
| TGCAAGTAAAGACACAACTT+TGG | - | chr7.4:10878999-10879018 | MS.gene061861:intron | 35.0% | |
| TGTCATCAAATTGTCCCAAA+TGG | + | chr7.4:10878500-10878519 | None:intergenic | 35.0% | |
| ! | AAAGTACCTTCACCAATGTT+GGG | + | chr7.4:10878797-10878816 | None:intergenic | 35.0% |
| ! | AGCTTGGATACAAAGTTTTG+AGG | + | chr7.4:10878248-10878267 | None:intergenic | 35.0% |
| !! | AAAAGTACCTTCACCAATGT+TGG | + | chr7.4:10878798-10878817 | None:intergenic | 35.0% |
| AAAAGTCAAGGTGTGGAGAA+TGG | - | chr7.4:10878341-10878360 | MS.gene061861:CDS | 40.0% | |
| AACAAAGGAACTTCGTGCAA+TGG | - | chr7.4:10879533-10879552 | MS.gene061861:CDS | 40.0% | |
| AAGAAGCAGTGGACATTCAT+TGG | - | chr7.4:10878366-10878385 | MS.gene061861:CDS | 40.0% | |
| ACAAAGGAACTTCGTGCAAT+GGG | - | chr7.4:10879534-10879553 | MS.gene061861:CDS | 40.0% | |
| ACCTCAATGCCATTCATGAT+AGG | + | chr7.4:10878434-10878453 | None:intergenic | 40.0% | |
| TGACCTAATTCTCATGGACA+TGG | - | chr7.4:10878403-10878422 | MS.gene061861:intron | 40.0% | |
| TGGATTGATCTTGCCAAGAA+TGG | + | chr7.4:10879679-10879698 | None:intergenic | 40.0% | |
| CTAAGCTACATCTTCAAGGG+AGG | - | chr7.4:10879299-10879318 | MS.gene061861:intron | 45.0% | |
| GCCTATCATGAATGGCATTG+AGG | - | chr7.4:10878430-10878449 | MS.gene061861:intron | 45.0% | |
| GGAGAATGGAAAAGAAGCAG+TGG | - | chr7.4:10878355-10878374 | MS.gene061861:CDS | 45.0% | |
| GGATCAACCCTGAACACTAA+AGG | + | chr7.4:10878971-10878990 | None:intergenic | 45.0% | |
| TGGAGTCAAAAGTCAAGGTG+TGG | - | chr7.4:10878334-10878353 | MS.gene061861:CDS | 45.0% | |
| TGTATCCAAGCTAACAGCAC+TGG | - | chr7.4:10878256-10878275 | MS.gene061861:CDS | 45.0% | |
| TTATGGCCCAACATTGGTGA+AGG | - | chr7.4:10878788-10878807 | MS.gene061861:intron | 45.0% | |
| !! | AGAAATAAATATTTATTTAT+AGG | - | chr7.4:10878861-10878880 | MS.gene061861:intron | 5.0% |
| !! | ATATAAAAATATATAAACTT+GGG | + | chr7.4:10878677-10878696 | None:intergenic | 5.0% |
| !!! | AATAAATATTTATTTTTTGA+AGG | - | chr7.4:10878834-10878853 | MS.gene061861:intron | 5.0% |
| !!! | TATATATTTTTATATGATTA+TGG | - | chr7.4:10878682-10878701 | MS.gene061861:intron | 5.0% |
| !!! | TTTTTAATAGTATTAATTTT+AGG | - | chr7.4:10879058-10879077 | MS.gene061861:intron | 5.0% |
| AGCTACATCTTCAAGGGAGG+AGG | - | chr7.4:10879302-10879321 | MS.gene061861:intron | 50.0% | |
| CAGTGGACATTCATTGGCGT+GGG | - | chr7.4:10878372-10878391 | MS.gene061861:CDS | 50.0% | |
| GTCTTTACTTGCAGGGTCGT+TGG | + | chr7.4:10878992-10879011 | None:intergenic | 50.0% | |
| TGGTGTGTCATCACGCTCTA+TGG | - | chr7.4:10879575-10879594 | MS.gene061861:CDS | 50.0% | |
| ! | CACAACCAGTGCTGTTAGCT+TGG | + | chr7.4:10878264-10878283 | None:intergenic | 50.0% |
| !! | CAAGCTAACAGCACTGGTTG+TGG | - | chr7.4:10878262-10878281 | MS.gene061861:CDS | 50.0% |
| GCAGTGGACATTCATTGGCG+TGG | - | chr7.4:10878371-10878390 | MS.gene061861:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 10878236 | 10879707 | 10878236 | ID=MS.gene061861 |
| chr7.4 | mRNA | 10878236 | 10879707 | 10878236 | ID=MS.gene061861.t1;Parent=MS.gene061861 |
| chr7.4 | exon | 10879492 | 10879707 | 10879492 | ID=MS.gene061861.t1.exon1;Parent=MS.gene061861.t1 |
| chr7.4 | CDS | 10879492 | 10879707 | 10879492 | ID=cds.MS.gene061861.t1;Parent=MS.gene061861.t1 |
| chr7.4 | exon | 10878236 | 10878412 | 10878236 | ID=MS.gene061861.t1.exon2;Parent=MS.gene061861.t1 |
| chr7.4 | CDS | 10878236 | 10878412 | 10878236 | ID=cds.MS.gene061861.t1;Parent=MS.gene061861.t1 |
| Gene Sequence |
| Protein sequence |
