AlfalfaGEDB | MS.gene061908

M. sativa cultivar XinJiangDaYe / MS.gene061908

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF13912.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061908.t1	XP_024627195.1	95.9	172	7	0	1	172	1	172	1.70E-65	258.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061908.t1	Q9SLD4	53.1	177	73	7	1	170	1	174	2.1e-19	97.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061908.t1	A0A396H512	95.9	172	7	0	1	172	1	172	1.3e-65	258.8

TFs/TRs:

Gene ID	Type	Classification
MS.gene061908.t1	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061908.t1	MTR_7g100100	68.263	167	39	4	1	166	3	156	1.01e-71	214
MS.gene061908.t1	MTR_1g067660	65.269	167	50	4	5	169	2	162	8.79e-71	211
MS.gene061908.t1	MTR_1g067650	61.364	176	51	7	1	169	2	167	1.50e-60	186
MS.gene061908.t1	MTR_1g067640	60.795	176	52	7	1	169	1	166	7.15e-60	184
MS.gene061908.t1	MTR_1g115345	39.901	203	81	8	1	168	1	197	8.80e-34	119
MS.gene061908.t1	MTR_7g023560	52.586	116	49	3	1	116	1	110	3.63e-32	114
MS.gene061908.t1	MTR_2g096540	48.667	150	51	6	9	157	4	128	7.02e-31	109
MS.gene061908.t1	MTR_2g084315	34.247	219	77	7	6	158	62	279	5.54e-24	95.5
MS.gene061908.t1	MTR_1g090703	34.450	209	72	8	11	156	98	304	8.44e-23	93.2
MS.gene061908.t1	MTR_1g062780	40.136	147	47	3	14	119	80	226	9.90e-23	92.0
MS.gene061908.t1	MTR_1g106730	50.000	100	44	2	15	114	60	153	8.22e-22	89.0
MS.gene061908.t1	MTR_4g057230	36.364	165	45	5	44	158	113	267	7.65e-21	87.0
MS.gene061908.t1	MTR_0160s0030	41.379	116	52	6	44	156	240	342	1.36e-16	76.3
MS.gene061908.t1	MTR_1g018420	38.462	117	55	2	15	114	43	159	1.41e-16	74.7
MS.gene061908.t1	MTR_1g077700	39.516	124	58	3	44	150	60	183	1.55e-16	73.6
MS.gene061908.t1	MTR_3g102980	40.171	117	49	3	15	114	52	164	1.07e-15	72.4
MS.gene061908.t1	MTR_1g077730	35.526	152	49	6	44	150	44	191	1.21e-15	71.2
MS.gene061908.t1	MTR_4g027440	37.903	124	59	4	44	150	220	342	1.32e-15	73.6
MS.gene061908.t1	MTR_5g062300	41.489	94	28	3	43	114	118	206	4.08e-11	60.1
MS.gene061908.t1	MTR_4g073310	37.647	85	45	1	30	114	19	95	5.61e-11	59.3
MS.gene061908.t1	MTR_3g023760	42.683	82	36	3	44	125	351	421	7.55e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061908.t1	AT2G37430	48.864	176	82	6	1	170	1	174	3.24e-46	149
MS.gene061908.t1	AT3G53600	45.556	180	79	6	1	170	1	171	2.46e-40	134
MS.gene061908.t1	AT2G28710	45.294	170	71	8	1	164	1	154	1.44e-33	116
MS.gene061908.t1	AT3G46090	43.023	172	79	6	1	163	2	163	8.70e-29	105
MS.gene061908.t1	AT5G59820	40.698	172	70	8	6	163	6	159	4.47e-27	100
MS.gene061908.t1	AT3G46080	39.181	171	85	5	1	163	2	161	1.65e-25	96.3
MS.gene061908.t1	AT2G28200	33.163	196	81	7	11	158	73	266	4.70e-25	98.2
MS.gene061908.t1	AT3G46070	34.857	175	76	6	1	155	1	157	1.53e-22	89.0
MS.gene061908.t1	AT5G03510	40.667	150	47	5	11	118	69	218	1.04e-21	89.4
MS.gene061908.t1	AT5G04390	29.870	231	83	7	8	159	93	323	8.03e-21	87.8
MS.gene061908.t1	AT2G45120	36.047	172	82	6	14	164	150	314	3.14e-20	85.9
MS.gene061908.t1	AT3G10470	45.192	104	26	3	44	116	182	285	5.74e-19	83.2
MS.gene061908.t1	AT3G60580	35.329	167	73	7	14	156	126	281	6.12e-19	82.0
MS.gene061908.t1	AT1G02030	50.000	78	38	1	44	120	160	237	6.62e-19	81.6
MS.gene061908.t1	AT4G35280	38.854	157	67	7	11	150	138	282	5.34e-18	79.3
MS.gene061908.t1	AT3G49930	38.393	112	57	3	15	114	60	171	1.96e-17	76.6
MS.gene061908.t1	AT3G19580	38.462	117	55	3	15	114	71	187	1.26e-16	75.5
MS.gene061908.t1	AT3G19580	38.462	117	55	3	15	114	71	187	1.26e-16	75.5
MS.gene061908.t1	AT5G43170	37.589	141	72	5	15	155	50	174	2.91e-16	73.2
MS.gene061908.t1	AT5G04340	48.780	82	31	2	44	114	89	170	8.86e-16	72.8
MS.gene061908.t1	AT2G17180	32.258	155	83	4	11	150	121	268	1.05e-14	70.1
MS.gene061908.t1	AT1G49900	40.367	109	50	4	21	114	159	267	1.74e-14	70.9
MS.gene061908.t1	AT1G49900	42.254	71	41	0	44	114	750	820	4.09e-13	66.6
MS.gene061908.t1	AT1G27730	39.815	108	53	3	15	114	55	158	2.29e-14	68.6
MS.gene061908.t1	AT5G67450	37.398	123	54	3	15	114	64	186	1.01e-13	67.0
MS.gene061908.t1	AT1G02040	36.170	141	53	3	11	114	107	247	1.54e-13	67.4
MS.gene061908.t1	AT5G56200	41.000	100	38	2	46	124	344	443	2.12e-13	67.4
MS.gene061908.t1	AT5G61470	43.056	72	39	1	43	114	227	296	2.52e-11	61.2

Find 29 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATATCTCTTTGTCTCTTCA+TGG	0.186634	7.2:-11953389	None:intergenic
TATTGTTATGTTGTTGATGT+TGG	0.324104	7.2:-11953465	None:intergenic
AATGATTTGAAGTTGTTGTT+TGG	0.347319	7.2:+11953853	MS.gene061908:CDS
ATTGTTATGTTGTTGATGTT+GGG	0.351718	7.2:-11953464	None:intergenic
CTATTCAATCTCTTTAAAAC+TGG	0.356656	7.2:-11953787	None:intergenic
AGGATTAGAAAGCATAGATT+TGG	0.368671	7.2:+11953414	MS.gene061908:CDS
CATAAAAGATCAAAGCTAGA+AGG	0.385523	7.2:+11953586	MS.gene061908:CDS
TTCATAACAACTTGCTCCAT+TGG	0.405809	7.2:-11953760	None:intergenic
AATTCTACATCTTTGAGCTT+AGG	0.417627	7.2:+11953625	MS.gene061908:CDS
GAAGTTGTTGTTTGGAAAGA+TGG	0.423340	7.2:+11953861	MS.gene061908:CDS
CAAACTAAAACATCAAGATT+AGG	0.441068	7.2:-11953886	None:intergenic
GGACTTGAACTTGACACCAT+TGG	0.443698	7.2:+11953828	MS.gene061908:CDS
AAGAGACAAAGAGATATAGA+AGG	0.447668	7.2:+11953394	MS.gene061908:CDS
TGCATGAATGTTCCATTTGT+GGG	0.475350	7.2:+11953662	MS.gene061908:CDS
TTGTTATGTTGTTGATGTTG+GGG	0.502259	7.2:-11953463	None:intergenic
ATGCATGAATGTTCCATTTG+TGG	0.513081	7.2:+11953661	MS.gene061908:CDS
TAAAGAGATTGAATAGTGCA+AGG	0.521881	7.2:+11953794	MS.gene061908:CDS
CAACGAAGAGGTTTCTCCAA+TGG	0.523977	7.2:+11953744	MS.gene061908:CDS
ATAAAAGATCAAAGCTAGAA+GGG	0.549441	7.2:+11953587	MS.gene061908:CDS
AAAGAGATTGAATAGTGCAA+GGG	0.553931	7.2:+11953795	MS.gene061908:CDS
GTGGCCACCTAGTGCTTGAA+AGG	0.555432	7.2:-11953554	None:intergenic
TAGTGCAAGGGTAATGTGCT+TGG	0.582174	7.2:+11953807	MS.gene061908:CDS
TTCTCATCCTTTCAAGCACT+AGG	0.596938	7.2:+11953547	MS.gene061908:CDS
TAAAGCTATTATCAACGAAG+AGG	0.599520	7.2:+11953732	MS.gene061908:CDS
AACTTCAAATCATTCTCCAA+TGG	0.606116	7.2:-11953844	None:intergenic
CATTGGGTGGACACATGAGA+AGG	0.615753	7.2:+11953707	MS.gene061908:CDS
TCATTGGGACAAGCATTGGG+TGG	0.622140	7.2:+11953694	MS.gene061908:CDS
TAAAAGATCAAAGCTAGAAG+GGG	0.632211	7.2:+11953588	MS.gene061908:CDS
TCATCCTTTCAAGCACTAGG+TGG	0.660073	7.2:+11953550	MS.gene061908:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATAAAAGATCAAAGCTAGAA+GGG	+	chr7.2:11953587-11953606	MS.gene061908:CDS	25.0%
!	ATTGTTATGTTGTTGATGTT+GGG	-	chr7.2:11953467-11953486	None:intergenic	25.0%
!	CAAACTAAAACATCAAGATT+AGG	-	chr7.2:11953889-11953908	None:intergenic	25.0%
!	TATTGTTATGTTGTTGATGT+TGG	-	chr7.2:11953468-11953487	None:intergenic	25.0%
!!	AATGATTTGAAGTTGTTGTT+TGG	+	chr7.2:11953853-11953872	MS.gene061908:CDS	25.0%
!!!	CTATTCAATCTCTTTAAAAC+TGG	-	chr7.2:11953790-11953809	None:intergenic	25.0%
	AAAGAGATTGAATAGTGCAA+GGG	+	chr7.2:11953795-11953814	MS.gene061908:CDS	30.0%
	AACTTCAAATCATTCTCCAA+TGG	-	chr7.2:11953847-11953866	None:intergenic	30.0%
	AAGAGACAAAGAGATATAGA+AGG	+	chr7.2:11953394-11953413	MS.gene061908:CDS	30.0%
	AATTCTACATCTTTGAGCTT+AGG	+	chr7.2:11953625-11953644	MS.gene061908:CDS	30.0%
	CATAAAAGATCAAAGCTAGA+AGG	+	chr7.2:11953586-11953605	MS.gene061908:CDS	30.0%
	TAAAAGATCAAAGCTAGAAG+GGG	+	chr7.2:11953588-11953607	MS.gene061908:CDS	30.0%
	TAAAGAGATTGAATAGTGCA+AGG	+	chr7.2:11953794-11953813	MS.gene061908:CDS	30.0%
	TAAAGCTATTATCAACGAAG+AGG	+	chr7.2:11953732-11953751	MS.gene061908:CDS	30.0%
	TTGTTATGTTGTTGATGTTG+GGG	-	chr7.2:11953466-11953485	None:intergenic	30.0%
!	AGGATTAGAAAGCATAGATT+TGG	+	chr7.2:11953414-11953433	MS.gene061908:CDS	30.0%
!	ATGGAACATTCATGCATTTT+AGG	-	chr7.2:11953658-11953677	None:intergenic	30.0%
!	TGAAAATTTTTGCCCACAAA+TGG	-	chr7.2:11953677-11953696	None:intergenic	30.0%
!	TGTGGGCAAAAATTTTCATT+GGG	+	chr7.2:11953679-11953698	MS.gene061908:CDS	30.0%
!	TTGTGGGCAAAAATTTTCAT+TGG	+	chr7.2:11953678-11953697	MS.gene061908:CDS	30.0%
!!!	CTTCTAGCTTTGATCTTTTA+TGG	-	chr7.2:11953588-11953607	None:intergenic	30.0%
	ATGCATGAATGTTCCATTTG+TGG	+	chr7.2:11953661-11953680	MS.gene061908:CDS	35.0%
	TGCATGAATGTTCCATTTGT+GGG	+	chr7.2:11953662-11953681	MS.gene061908:CDS	35.0%
!	ATTTTCATTGGGACAAGCAT+TGG	+	chr7.2:11953690-11953709	MS.gene061908:CDS	35.0%
!	TTCATAACAACTTGCTCCAT+TGG	-	chr7.2:11953763-11953782	None:intergenic	35.0%
!	TTTTCATTGGGACAAGCATT+GGG	+	chr7.2:11953691-11953710	MS.gene061908:CDS	35.0%
!!	GAAGTTGTTGTTTGGAAAGA+TGG	+	chr7.2:11953861-11953880	MS.gene061908:CDS	35.0%
!!!	GGCGCAAAACTTTTTTGATT+TGG	-	chr7.2:11953493-11953512	None:intergenic	35.0%
	TTCTCATCCTTTCAAGCACT+AGG	+	chr7.2:11953547-11953566	MS.gene061908:CDS	40.0%
!	GTTTTGCACTCATATTCCAC+TGG	-	chr7.2:11953514-11953533	None:intergenic	40.0%
!	TCAAAAAAGTTTTGCGCCAG+TGG	+	chr7.2:11953495-11953514	MS.gene061908:CDS	40.0%
!!!	ATCTTTTATGGCTAGCTCTG+TGG	-	chr7.2:11953576-11953595	None:intergenic	40.0%
	CAACGAAGAGGTTTCTCCAA+TGG	+	chr7.2:11953744-11953763	MS.gene061908:CDS	45.0%
	GGACTTGAACTTGACACCAT+TGG	+	chr7.2:11953828-11953847	MS.gene061908:CDS	45.0%
	TAGTGCAAGGGTAATGTGCT+TGG	+	chr7.2:11953807-11953826	MS.gene061908:CDS	45.0%
	TCATCCTTTCAAGCACTAGG+TGG	+	chr7.2:11953550-11953569	MS.gene061908:CDS	45.0%
	CATTGGGTGGACACATGAGA+AGG	+	chr7.2:11953707-11953726	MS.gene061908:CDS	50.0%
!!	TCATTGGGACAAGCATTGGG+TGG	+	chr7.2:11953694-11953713	MS.gene061908:CDS	50.0%
	GTGGCCACCTAGTGCTTGAA+AGG	-	chr7.2:11953557-11953576	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	11953391	11953909	11953391	ID=MS.gene061908
chr7.2	mRNA	11953391	11953909	11953391	ID=MS.gene061908.t1;Parent=MS.gene061908
chr7.2	exon	11953391	11953909	11953391	ID=MS.gene061908.t1.exon1;Parent=MS.gene061908.t1
chr7.2	CDS	11953391	11953909	11953391	ID=cds.MS.gene061908.t1;Parent=MS.gene061908.t1

Gene Sequence

>MS.gene061908

ATGAAGAGACAAAGAGATATAGAAGGATTAGAAAGCATAGATTTGGCAAATTGTCTAATGATGCTCTCTTATCCCCAACATCAACAACATAACAATAAACCAAATCAAAAAAGTTTTGCGCCAGTGGAATATGAGTGCAAAACATGCAACAAAAAATTCTCATCCTTTCAAGCACTAGGTGGCCACAGAGCTAGCCATAAAAGATCAAAGCTAGAAGGGGATGAATTACTAACAAATTCTACATCTTTGAGCTTAGGAAATACGCCTAAAATGCATGAATGTTCCATTTGTGGGCAAAAATTTTCATTGGGACAAGCATTGGGTGGACACATGAGAAGGCATAAAGCTATTATCAACGAAGAGGTTTCTCCAATGGAGCAAGTTGTTATGAAATTACCAGTTTTAAAGAGATTGAATAGTGCAAGGGTAATGTGCTTGGACTTGAACTTGACACCATTGGAGAATGATTTGAAGTTGTTGTTTGGAAAGATGGCTCCTAATCTTGATGTTTTAGTTTGA

Protein sequence

>MS.gene061908.t1

MKRQRDIEGLESIDLANCLMMLSYPQHQQHNNKPNQKSFAPVEYECKTCNKKFSSFQALGGHRASHKRSKLEGDELLTNSTSLSLGNTPKMHECSICGQKFSLGQALGGHMRRHKAIINEEVSPMEQVVMKLPVLKRLNSARVMCLDLNLTPLENDLKLLFGKMAPNLDVLV