Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061951.t1 | RHN67031.1 | 80.4 | 184 | 27 | 1 | 17 | 200 | 3 | 177 | 1.00E-86 | 329.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061951.t1 | Q9LT81 | 37.0 | 246 | 96 | 7 | 8 | 205 | 68 | 302 | 2.7e-40 | 166.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061951.t1 | G7J1X0 | 80.4 | 184 | 27 | 1 | 17 | 200 | 3 | 177 | 7.3e-87 | 329.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene061951.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061951.t1 | MTR_3g050390 | 80.435 | 184 | 27 | 1 | 17 | 200 | 3 | 177 | 5.44e-108 | 310 |
| MS.gene061951.t1 | MTR_4g113970 | 54.393 | 239 | 74 | 7 | 3 | 207 | 17 | 254 | 6.80e-77 | 232 |
| MS.gene061951.t1 | MTR_4g113860 | 49.558 | 226 | 85 | 5 | 3 | 200 | 17 | 241 | 2.08e-64 | 202 |
| MS.gene061951.t1 | MTR_4g113940 | 48.357 | 213 | 90 | 7 | 1 | 199 | 1 | 207 | 2.40e-56 | 180 |
| MS.gene061951.t1 | MTR_4g113890 | 41.121 | 214 | 72 | 6 | 1 | 199 | 1 | 175 | 6.67e-42 | 142 |
| MS.gene061951.t1 | MTR_8g066730 | 32.161 | 199 | 89 | 4 | 42 | 197 | 86 | 281 | 6.48e-26 | 102 |
| MS.gene061951.t1 | MTR_8g066730 | 31.019 | 216 | 103 | 4 | 42 | 214 | 86 | 298 | 1.20e-25 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061951.t1 | AT3G19360 | 37.344 | 241 | 92 | 7 | 8 | 200 | 68 | 297 | 2.84e-45 | 155 |
| MS.gene061951.t1 | AT3G19360 | 37.344 | 241 | 92 | 7 | 8 | 200 | 68 | 297 | 2.84e-45 | 155 |
| MS.gene061951.t1 | AT1G32360 | 29.817 | 218 | 102 | 4 | 41 | 210 | 84 | 298 | 1.80e-24 | 99.8 |
Find 70 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGACGAATGGATGCAGTTTC+TGG | 0.174701 | 5.3:+51579937 | MS.gene061951:CDS |
| CGGAGATGGTTCTAATAATT+TGG | 0.195023 | 5.3:+51580114 | MS.gene061951:CDS |
| CTGCCTGTTTCTTTGTTTGA+TGG | 0.234171 | 5.3:-51579776 | None:intergenic |
| CTAATAATTTGGAAGGCAAT+AGG | 0.289690 | 5.3:+51580125 | MS.gene061951:CDS |
| AAACAGGGCGCTGTCCCTTT+GGG | 0.311148 | 5.3:+51580209 | MS.gene061951:CDS |
| CTCTTGTTCATTGGAAATTG+AGG | 0.339686 | 5.3:-51579704 | None:intergenic |
| ATAGGCTTAGGGATGATTCT+TGG | 0.341417 | 5.3:+51580053 | MS.gene061951:CDS |
| CAAACAGGGCGCTGTCCCTT+TGG | 0.343335 | 5.3:+51580208 | MS.gene061951:CDS |
| ATTATTGGCCTCTTGTTCAT+TGG | 0.344534 | 5.3:-51579713 | None:intergenic |
| TAGTTACAATATCCACCTTT+AGG | 0.349503 | 5.3:-51579848 | None:intergenic |
| TGTCCCATGTGGCAGCAAAC+AGG | 0.365446 | 5.3:+51580193 | MS.gene061951:CDS |
| CTCGTATTTGTCATAAGTTT+AGG | 0.369779 | 5.3:+51579813 | MS.gene061951:CDS |
| AGGCAGCAAGGGGAACTTAC+TGG | 0.380896 | 5.3:+51580158 | MS.gene061951:CDS |
| GGTAATGATAATACTGGTCT+TGG | 0.407298 | 5.3:+51580262 | MS.gene061951:CDS |
| TTTGGGGAGGACTGTCACTA+TGG | 0.414198 | 5.3:+51580226 | MS.gene061951:CDS |
| TAAGGCAGCTACCACCGAAT+TGG | 0.416824 | 5.3:+51579891 | MS.gene061951:CDS |
| TCTTGTTCATTGGAAATTGA+GGG | 0.426338 | 5.3:-51579703 | None:intergenic |
| GTTTCTTTGTTTGATGGAGG+AGG | 0.433415 | 5.3:-51579770 | None:intergenic |
| TAATAATTTGGAAGGCAATA+GGG | 0.433859 | 5.3:+51580126 | MS.gene061951:CDS |
| AGGTTTGGTAATTGTCCTAA+AGG | 0.435581 | 5.3:+51579833 | MS.gene061951:CDS |
| ATTTGTCATAAGTTTAGGTT+TGG | 0.439137 | 5.3:+51579818 | MS.gene061951:CDS |
| AAGATATTGTTATGGTGGTC+TGG | 0.443867 | 5.3:-51579671 | None:intergenic |
| AGGCAATAGGGCTGTGATCA+AGG | 0.451978 | 5.3:+51580138 | MS.gene061951:CDS |
| AATGGTGAGGAATGTCCTTA+TGG | 0.454214 | 5.3:+51580010 | MS.gene061951:CDS |
| GAAGCAGGTAATGATAATAC+TGG | 0.456961 | 5.3:+51580256 | MS.gene061951:CDS |
| AGGAGGGCATATGAATTCAT+TGG | 0.458224 | 5.3:-51579753 | None:intergenic |
| CCTCCATCAAACAAAGAAAC+AGG | 0.458374 | 5.3:+51579773 | MS.gene061951:CDS |
| CAGAAAATCATTCACAAGAT+GGG | 0.463529 | 5.3:+51579968 | MS.gene061951:CDS |
| CTCATGGGGTTGGTGAGATA+AGG | 0.466573 | 5.3:+51579873 | MS.gene061951:CDS |
| TCAGAAAATCATTCACAAGA+TGG | 0.468298 | 5.3:+51579967 | MS.gene061951:CDS |
| CCAGCTACCTCCTTCCAATT+CGG | 0.472104 | 5.3:-51579905 | None:intergenic |
| CCTGTTTCTTTGTTTGATGG+AGG | 0.476598 | 5.3:-51579773 | None:intergenic |
| CACTATGGTCATGCAGAAGC+AGG | 0.481354 | 5.3:+51580241 | MS.gene061951:CDS |
| TTTCTTTGTTTGATGGAGGA+GGG | 0.488896 | 5.3:-51579769 | None:intergenic |
| AAACTACACTTATCACCATA+AGG | 0.492600 | 5.3:-51580025 | None:intergenic |
| CGCCCTGTTTGCTGCCACAT+GGG | 0.493119 | 5.3:-51580196 | None:intergenic |
| GATATTGTAACTATGCTCAT+GGG | 0.511020 | 5.3:+51579858 | MS.gene061951:CDS |
| GGATATTGTAACTATGCTCA+TGG | 0.512690 | 5.3:+51579857 | MS.gene061951:CDS |
| TCGCTACAAAGATATTGTTA+TGG | 0.514013 | 5.3:-51579679 | None:intergenic |
| CTGGCCCTCGCAACGACGAA+TGG | 0.515528 | 5.3:+51579924 | MS.gene061951:CDS |
| GCGCCCTGTTTGCTGCCACA+TGG | 0.515715 | 5.3:-51580197 | None:intergenic |
| GATGGTTCTAATAATTTGGA+AGG | 0.521303 | 5.3:+51580118 | MS.gene061951:CDS |
| GTCCCATGTGGCAGCAAACA+GGG | 0.525337 | 5.3:+51580194 | MS.gene061951:CDS |
| CCGAATTGGAAGGAGGTAGC+TGG | 0.528816 | 5.3:+51579905 | MS.gene061951:CDS |
| CTCAATTTCCAATGAACAAG+AGG | 0.531343 | 5.3:+51579705 | MS.gene061951:CDS |
| GCATTAAGCATTGGAAGTAT+CGG | 0.533941 | 5.3:+51580094 | MS.gene061951:CDS |
| GGCTGTGATCAAGGCAGCAA+GGG | 0.543600 | 5.3:+51580147 | MS.gene061951:CDS |
| TAGTGACAGTCCTCCCCAAA+GGG | 0.553368 | 5.3:-51580223 | None:intergenic |
| AACAGGGCGCTGTCCCTTTG+GGG | 0.556253 | 5.3:+51580210 | MS.gene061951:CDS |
| GAGGCCTATGCATTAAGCAT+TGG | 0.556818 | 5.3:+51580085 | MS.gene061951:CDS |
| ACTTCCAATGCTTAATGCAT+AGG | 0.559099 | 5.3:-51580089 | None:intergenic |
| GGGCTGTGATCAAGGCAGCA+AGG | 0.562998 | 5.3:+51580146 | MS.gene061951:CDS |
| GCATCCATTCGTCGTTGCGA+GGG | 0.574844 | 5.3:-51579928 | None:intergenic |
| TGCATCCATTCGTCGTTGCG+AGG | 0.576815 | 5.3:-51579929 | None:intergenic |
| CTACAAAGATATTGTTATGG+TGG | 0.577110 | 5.3:-51579676 | None:intergenic |
| TTTGGTAATTGTCCTAAAGG+TGG | 0.589355 | 5.3:+51579836 | MS.gene061951:CDS |
| AGCATTGGAAGTATCGGAGA+TGG | 0.590771 | 5.3:+51580100 | MS.gene061951:CDS |
| TGTAACTATGCTCATGGGGT+TGG | 0.592945 | 5.3:+51579863 | MS.gene061951:CDS |
| AGGGCGCTGTCCCTTTGGGG+AGG | 0.602360 | 5.3:+51580213 | MS.gene061951:CDS |
| AGAAAATCATTCACAAGATG+GGG | 0.602501 | 5.3:+51579969 | MS.gene061951:CDS |
| GCAGCTACCACCGAATTGGA+AGG | 0.620245 | 5.3:+51579895 | MS.gene061951:CDS |
| GCTACCTCCTTCCAATTCGG+TGG | 0.638241 | 5.3:-51579902 | None:intergenic |
| ATAGTGACAGTCCTCCCCAA+AGG | 0.646765 | 5.3:-51580224 | None:intergenic |
| CAAGAAATACTACAATGGTG+AGG | 0.666991 | 5.3:+51579997 | MS.gene061951:CDS |
| GCTGTGATCAAGGCAGCAAG+GGG | 0.668444 | 5.3:+51580148 | MS.gene061951:CDS |
| CTATGCAAGAAATACTACAA+TGG | 0.669884 | 5.3:+51579992 | MS.gene061951:CDS |
| TGATTCTTGGAAATCAAGAG+AGG | 0.676002 | 5.3:+51580066 | MS.gene061951:CDS |
| GCTACCACCGAATTGGAAGG+AGG | 0.676137 | 5.3:+51579898 | MS.gene061951:CDS |
| ATATTGTAACTATGCTCATG+GGG | 0.682160 | 5.3:+51579859 | MS.gene061951:CDS |
| AGATTAAGCTGTGTCCCATG+TGG | 0.762737 | 5.3:+51580182 | MS.gene061951:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGATTGTTTTCAGAAATTAT+TGG | - | chr5.3:51579731-51579750 | None:intergenic | 20.0% |
| ! | TAATAATTTGGAAGGCAATA+GGG | + | chr5.3:51580126-51580145 | MS.gene061951:CDS | 25.0% |
| !! | ATAAGTGTAGTTTTCTTCAT+AGG | + | chr5.3:51580035-51580054 | MS.gene061951:CDS | 25.0% |
| !! | ATTTGTCATAAGTTTAGGTT+TGG | + | chr5.3:51579818-51579837 | MS.gene061951:CDS | 25.0% |
| AAACTACACTTATCACCATA+AGG | - | chr5.3:51580028-51580047 | None:intergenic | 30.0% | |
| AGAAAATCATTCACAAGATG+GGG | + | chr5.3:51579969-51579988 | MS.gene061951:CDS | 30.0% | |
| ATATTGTAACTATGCTCATG+GGG | + | chr5.3:51579859-51579878 | MS.gene061951:CDS | 30.0% | |
| CAGAAAATCATTCACAAGAT+GGG | + | chr5.3:51579968-51579987 | MS.gene061951:CDS | 30.0% | |
| CTAATAATTTGGAAGGCAAT+AGG | + | chr5.3:51580125-51580144 | MS.gene061951:CDS | 30.0% | |
| CTACAAAGATATTGTTATGG+TGG | - | chr5.3:51579679-51579698 | None:intergenic | 30.0% | |
| CTATGCAAGAAATACTACAA+TGG | + | chr5.3:51579992-51580011 | MS.gene061951:CDS | 30.0% | |
| CTCGTATTTGTCATAAGTTT+AGG | + | chr5.3:51579813-51579832 | MS.gene061951:CDS | 30.0% | |
| GATATTGTAACTATGCTCAT+GGG | + | chr5.3:51579858-51579877 | MS.gene061951:CDS | 30.0% | |
| TAGTTACAATATCCACCTTT+AGG | - | chr5.3:51579851-51579870 | None:intergenic | 30.0% | |
| TCAGAAAATCATTCACAAGA+TGG | + | chr5.3:51579967-51579986 | MS.gene061951:CDS | 30.0% | |
| TCGCTACAAAGATATTGTTA+TGG | - | chr5.3:51579682-51579701 | None:intergenic | 30.0% | |
| TCTTGTTCATTGGAAATTGA+GGG | - | chr5.3:51579706-51579725 | None:intergenic | 30.0% | |
| ! | TAGTTTTCTTCATAGGCTTA+GGG | + | chr5.3:51580042-51580061 | MS.gene061951:CDS | 30.0% |
| !! | GATGGTTCTAATAATTTGGA+AGG | + | chr5.3:51580118-51580137 | MS.gene061951:CDS | 30.0% |
| AAGATATTGTTATGGTGGTC+TGG | - | chr5.3:51579674-51579693 | None:intergenic | 35.0% | |
| ACTTCCAATGCTTAATGCAT+AGG | - | chr5.3:51580092-51580111 | None:intergenic | 35.0% | |
| CAAGAAATACTACAATGGTG+AGG | + | chr5.3:51579997-51580016 | MS.gene061951:CDS | 35.0% | |
| CTCAATTTCCAATGAACAAG+AGG | + | chr5.3:51579705-51579724 | MS.gene061951:CDS | 35.0% | |
| CTCTTGTTCATTGGAAATTG+AGG | - | chr5.3:51579707-51579726 | None:intergenic | 35.0% | |
| GAAGCAGGTAATGATAATAC+TGG | + | chr5.3:51580256-51580275 | MS.gene061951:CDS | 35.0% | |
| GGATATTGTAACTATGCTCA+TGG | + | chr5.3:51579857-51579876 | MS.gene061951:CDS | 35.0% | |
| TGATTCTTGGAAATCAAGAG+AGG | + | chr5.3:51580066-51580085 | MS.gene061951:CDS | 35.0% | |
| ! | AGGTTTGGTAATTGTCCTAA+AGG | + | chr5.3:51579833-51579852 | MS.gene061951:CDS | 35.0% |
| ! | ATTATTGGCCTCTTGTTCAT+TGG | - | chr5.3:51579716-51579735 | None:intergenic | 35.0% |
| ! | GGTAATGATAATACTGGTCT+TGG | + | chr5.3:51580262-51580281 | MS.gene061951:CDS | 35.0% |
| ! | GTAGTTTTCTTCATAGGCTT+AGG | + | chr5.3:51580041-51580060 | MS.gene061951:CDS | 35.0% |
| ! | TTTCTTTGTTTGATGGAGGA+GGG | - | chr5.3:51579772-51579791 | None:intergenic | 35.0% |
| ! | TTTGGTAATTGTCCTAAAGG+TGG | + | chr5.3:51579836-51579855 | MS.gene061951:CDS | 35.0% |
| !! | CGGAGATGGTTCTAATAATT+TGG | + | chr5.3:51580114-51580133 | MS.gene061951:CDS | 35.0% |
| !! | GCATTAAGCATTGGAAGTAT+CGG | + | chr5.3:51580094-51580113 | MS.gene061951:CDS | 35.0% |
| AATGGTGAGGAATGTCCTTA+TGG | + | chr5.3:51580010-51580029 | MS.gene061951:CDS | 40.0% | |
| ATAGGCTTAGGGATGATTCT+TGG | + | chr5.3:51580053-51580072 | MS.gene061951:CDS | 40.0% | |
| CCTCCATCAAACAAAGAAAC+AGG | + | chr5.3:51579773-51579792 | MS.gene061951:CDS | 40.0% | |
| ! | AGGAGGGCATATGAATTCAT+TGG | - | chr5.3:51579756-51579775 | None:intergenic | 40.0% |
| ! | CCTGTTTCTTTGTTTGATGG+AGG | - | chr5.3:51579776-51579795 | None:intergenic | 40.0% |
| ! | CTGCCTGTTTCTTTGTTTGA+TGG | - | chr5.3:51579779-51579798 | None:intergenic | 40.0% |
| ! | GTTTCTTTGTTTGATGGAGG+AGG | - | chr5.3:51579773-51579792 | None:intergenic | 40.0% |
| AGATTAAGCTGTGTCCCATG+TGG | + | chr5.3:51580182-51580201 | MS.gene061951:CDS | 45.0% | |
| GAGGCCTATGCATTAAGCAT+TGG | + | chr5.3:51580085-51580104 | MS.gene061951:CDS | 45.0% | |
| TGTAACTATGCTCATGGGGT+TGG | + | chr5.3:51579863-51579882 | MS.gene061951:CDS | 45.0% | |
| !! | AGCATTGGAAGTATCGGAGA+TGG | + | chr5.3:51580100-51580119 | MS.gene061951:CDS | 45.0% |
| AGGCAATAGGGCTGTGATCA+AGG | + | chr5.3:51580138-51580157 | MS.gene061951:CDS | 50.0% | |
| ATAGTGACAGTCCTCCCCAA+AGG | - | chr5.3:51580227-51580246 | None:intergenic | 50.0% | |
| CACTATGGTCATGCAGAAGC+AGG | + | chr5.3:51580241-51580260 | MS.gene061951:CDS | 50.0% | |
| CCAGCTACCTCCTTCCAATT+CGG | - | chr5.3:51579908-51579927 | None:intergenic | 50.0% | |
| CGACGAATGGATGCAGTTTC+TGG | + | chr5.3:51579937-51579956 | MS.gene061951:CDS | 50.0% | |
| TAAGGCAGCTACCACCGAAT+TGG | + | chr5.3:51579891-51579910 | MS.gene061951:CDS | 50.0% | |
| TAGTGACAGTCCTCCCCAAA+GGG | - | chr5.3:51580226-51580245 | None:intergenic | 50.0% | |
| TTTGGGGAGGACTGTCACTA+TGG | + | chr5.3:51580226-51580245 | MS.gene061951:CDS | 50.0% | |
| !! | CTCATGGGGTTGGTGAGATA+AGG | + | chr5.3:51579873-51579892 | MS.gene061951:CDS | 50.0% |
| AGGCAGCAAGGGGAACTTAC+TGG | + | chr5.3:51580158-51580177 | MS.gene061951:CDS | 55.0% | |
| CCGAATTGGAAGGAGGTAGC+TGG | + | chr5.3:51579905-51579924 | MS.gene061951:CDS | 55.0% | |
| GCAGCTACCACCGAATTGGA+AGG | + | chr5.3:51579895-51579914 | MS.gene061951:CDS | 55.0% | |
| GCATCCATTCGTCGTTGCGA+GGG | - | chr5.3:51579931-51579950 | None:intergenic | 55.0% | |
| GCTACCACCGAATTGGAAGG+AGG | + | chr5.3:51579898-51579917 | MS.gene061951:CDS | 55.0% | |
| GCTACCTCCTTCCAATTCGG+TGG | - | chr5.3:51579905-51579924 | None:intergenic | 55.0% | |
| GCTGTGATCAAGGCAGCAAG+GGG | + | chr5.3:51580148-51580167 | MS.gene061951:CDS | 55.0% | |
| GGCTGTGATCAAGGCAGCAA+GGG | + | chr5.3:51580147-51580166 | MS.gene061951:CDS | 55.0% | |
| GTCCCATGTGGCAGCAAACA+GGG | + | chr5.3:51580194-51580213 | MS.gene061951:CDS | 55.0% | |
| TGCATCCATTCGTCGTTGCG+AGG | - | chr5.3:51579932-51579951 | None:intergenic | 55.0% | |
| TGTCCCATGTGGCAGCAAAC+AGG | + | chr5.3:51580193-51580212 | MS.gene061951:CDS | 55.0% | |
| !! | AAACAGGGCGCTGTCCCTTT+GGG | + | chr5.3:51580209-51580228 | MS.gene061951:CDS | 55.0% |
| GGGCTGTGATCAAGGCAGCA+AGG | + | chr5.3:51580146-51580165 | MS.gene061951:CDS | 60.0% | |
| ! | CGCCCTGTTTGCTGCCACAT+GGG | - | chr5.3:51580199-51580218 | None:intergenic | 60.0% |
| !! | AACAGGGCGCTGTCCCTTTG+GGG | + | chr5.3:51580210-51580229 | MS.gene061951:CDS | 60.0% |
| !! | CAAACAGGGCGCTGTCCCTT+TGG | + | chr5.3:51580208-51580227 | MS.gene061951:CDS | 60.0% |
| CTGGCCCTCGCAACGACGAA+TGG | + | chr5.3:51579924-51579943 | MS.gene061951:CDS | 65.0% | |
| ! | GCGCCCTGTTTGCTGCCACA+TGG | - | chr5.3:51580200-51580219 | None:intergenic | 65.0% |
| !! | AGGGCGCTGTCCCTTTGGGG+AGG | + | chr5.3:51580213-51580232 | MS.gene061951:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 51579662 | 51580318 | 51579662 | ID=MS.gene061951 |
| chr5.3 | mRNA | 51579662 | 51580318 | 51579662 | ID=MS.gene061951.t1;Parent=MS.gene061951 |
| chr5.3 | exon | 51579662 | 51580318 | 51579662 | ID=MS.gene061951.t1.exon1;Parent=MS.gene061951.t1 |
| chr5.3 | CDS | 51579662 | 51580318 | 51579662 | ID=cds.MS.gene061951.t1;Parent=MS.gene061951.t1 |
| Gene Sequence |
| Protein sequence |
