AlfalfaGEDB | MS.gene062172

M. sativa cultivar XinJiangDaYe / MS.gene062172

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF13912.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062172.t1	XP_013468856.1	63	189	61	4	1	183	1	186	1.70E-47	199.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062172.t1	Q681X4	37.0	154	67	4	56	181	108	259	1.3e-14	81.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062172.t1	A0A072VND0	63.0	189	61	4	1	183	1	186	1.3e-47	199.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene062172.t1	TF	C2H2

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062172.t1	MTR_1g077730	58.879	214	37	6	1	183	1	194	3.52e-71	214
MS.gene062172.t1	MTR_1g077700	69.110	191	46	5	1	183	1	186	2.22e-69	209
MS.gene062172.t1	MTR_1g090703	51.190	168	49	7	44	181	135	299	2.76e-37	132
MS.gene062172.t1	MTR_1g067640	38.710	124	60	3	63	182	38	149	1.15e-19	81.6
MS.gene062172.t1	MTR_1g067650	38.710	124	60	3	63	182	39	150	1.32e-19	81.6
MS.gene062172.t1	MTR_7g100100	40.650	123	57	4	63	182	34	143	1.41e-19	81.3
MS.gene062172.t1	MTR_2g084315	36.184	152	66	4	60	181	122	272	1.53e-18	81.3
MS.gene062172.t1	MTR_1g115345	46.914	81	37	1	62	142	44	118	6.21e-18	78.2
MS.gene062172.t1	MTR_1g062780	34.839	155	69	3	56	181	113	264	1.90e-17	78.2
MS.gene062172.t1	MTR_7g023560	49.398	83	31	2	62	144	39	110	1.10e-16	74.3
MS.gene062172.t1	MTR_2g096540	33.333	132	60	2	47	178	18	121	1.79e-16	72.4
MS.gene062172.t1	MTR_1g067660	38.710	124	59	5	63	182	35	145	6.31e-16	71.6
MS.gene062172.t1	MTR_4g027440	41.975	81	46	1	63	142	220	300	6.49e-14	68.9
MS.gene062172.t1	MTR_4g028610	34.416	154	66	6	62	181	226	378	1.37e-13	68.2
MS.gene062172.t1	MTR_1g018420	33.613	119	72	2	63	181	81	192	6.23e-12	62.4
MS.gene062172.t1	MTR_1g106730	40.000	80	39	1	63	142	83	153	8.20e-12	62.4
MS.gene062172.t1	MTR_0160s0030	33.871	124	60	4	58	181	235	336	1.77e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062172.t1	AT3G10470	42.775	173	48	8	58	181	177	347	1.75e-28	109
MS.gene062172.t1	AT2G28200	42.208	154	59	6	56	181	108	259	9.31e-27	103
MS.gene062172.t1	AT5G04390	41.081	185	60	8	41	181	136	315	9.42e-27	104
MS.gene062172.t1	AT2G37430	40.602	133	56	5	56	182	40	155	6.09e-20	82.8
MS.gene062172.t1	AT5G03510	36.620	142	70	3	61	182	116	257	1.33e-18	81.3
MS.gene062172.t1	AT3G53600	31.677	161	86	5	23	182	15	152	1.16e-17	76.6
MS.gene062172.t1	AT5G59820	30.899	178	87	6	8	180	1	147	2.59e-16	72.8
MS.gene062172.t1	AT3G46090	36.585	123	63	2	61	179	38	149	4.67e-16	72.4
MS.gene062172.t1	AT3G46080	43.902	82	37	1	61	142	35	107	6.66e-15	69.3
MS.gene062172.t1	AT2G28710	39.216	102	43	3	41	142	18	100	1.45e-14	68.2
MS.gene062172.t1	AT3G49930	42.353	85	37	2	63	142	94	171	8.16e-14	67.4
MS.gene062172.t1	AT3G60580	47.500	80	36	2	63	142	173	246	1.67e-13	67.4
MS.gene062172.t1	AT2G45120	35.294	119	64	2	63	181	194	299	2.43e-13	67.0
MS.gene062172.t1	AT3G46070	35.870	92	52	1	51	142	24	108	8.62e-13	63.5
MS.gene062172.t1	AT1G02030	38.372	86	45	1	59	144	156	233	9.20e-13	65.1
MS.gene062172.t1	AT5G04340	38.554	83	49	1	62	142	88	170	1.24e-12	64.3
MS.gene062172.t1	AT5G43170	43.750	80	31	3	63	142	75	140	2.41e-12	62.8
MS.gene062172.t1	AT5G67450	38.889	90	45	2	63	142	97	186	4.96e-12	62.8
MS.gene062172.t1	AT1G27730	43.210	81	43	2	63	142	80	158	8.95e-12	62.0
MS.gene062172.t1	AT4G35280	33.333	132	64	3	54	182	153	263	1.01e-11	62.4
MS.gene062172.t1	AT5G61470	39.785	93	44	3	50	142	216	296	2.55e-11	61.2
MS.gene062172.t1	AT3G19580	36.585	82	50	1	63	142	106	187	3.87e-11	60.5
MS.gene062172.t1	AT3G19580	36.585	82	50	1	63	142	106	187	3.87e-11	60.5
MS.gene062172.t1	AT5G56200	38.144	97	47	3	63	146	342	438	4.61e-11	61.2
MS.gene062172.t1	AT2G17180	31.429	140	69	4	50	183	135	253	7.41e-11	59.7

Find 0 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CAAAGTGAATATAACTCAAA+AGG	-	11149:66948-66967	MS.gene062172:CDS	25.0%
!!!	CTTTTGAGTTATATTCACTT+TGG	+	11149:66950-66969	MS.gene062172:intergenic	25.0%
	AACAACAAATCCAACGTTTA+TGG	-	11149:67225-67244	MS.gene062172:CDS	30.0%
	ACAACAAATCCAACGTTTAT+GGG	-	11149:67226-67245	MS.gene062172:CDS	30.0%
	AGAAAAAACGAATGTCTCTT+TGG	-	11149:67386-67405	MS.gene062172:CDS	30.0%
	AGTAGAAGAATAAGCAATGA+TGG	+	11149:67007-67026	MS.gene062172:intergenic	30.0%
!	AATAAGCAATGATGGAGAAA+GGG	+	11149:66999-67018	MS.gene062172:intergenic	30.0%
!	ATTCACTTTGGTGACATTAA+TGG	+	11149:66938-66957	MS.gene062172:intergenic	30.0%
	AACGAATGTCTCTTTGGATT+TGG	-	11149:67392-67411	MS.gene062172:CDS	35.0%
	CCTAGAAAAGCTCATAGAAA+AGG	-	11149:67147-67166	MS.gene062172:CDS	35.0%
	GCTTATTCTTCTACTAGAGA+AGG	-	11149:67012-67031	MS.gene062172:CDS	35.0%
	TATTCTTCTACTAGAGAAGG+AGG	-	11149:67015-67034	MS.gene062172:CDS	35.0%
	TTCACTTCATTTCAAGCACT+TGG	-	11149:67099-67118	MS.gene062172:CDS	35.0%
!	CCTTTTCTATGAGCTTTTCT+AGG	+	11149:67150-67169	MS.gene062172:intergenic	35.0%
!	GAATAAGCAATGATGGAGAA+AGG	+	11149:67000-67019	MS.gene062172:intergenic	35.0%
!	GCTTCTTGTGAACTTTTCTA+TGG	+	11149:67128-67147	MS.gene062172:intergenic	35.0%
!	GTTGTTGTTGTTGTTGTTGT+TGG	+	11149:67211-67230	MS.gene062172:intergenic	35.0%
!!	TGTGGTTCTAAATTCACATC+TGG	-	11149:67258-67277	MS.gene062172:CDS	35.0%
	ACTTCATTTCAAGCACTTGG+AGG	-	11149:67102-67121	MS.gene062172:CDS	40.0%
	AGAAAGGGATAGGAGATTGT+GGG	+	11149:66984-67003	MS.gene062172:intergenic	40.0%
	CTCATTAACACCAATGGCTT+TGG	-	11149:67332-67351	MS.gene062172:CDS	40.0%
	GGTTCTTCATCTTCTTTGTC+TGG	+	11149:67360-67379	MS.gene062172:intergenic	40.0%
	TTCTAAATTCACATCTGGCC+AGG	-	11149:67263-67282	MS.gene062172:CDS	40.0%
!	GTTGTTGTTGTTGTTGGTAG+TGG	+	11149:67205-67224	MS.gene062172:intergenic	40.0%
!!	CAAAGCCATTGGTGTTAATG+AGG	+	11149:67334-67353	MS.gene062172:intergenic	40.0%
!!!	ACATTCGTTTTTTCTGCAGG+TGG	+	11149:67381-67400	MS.gene062172:intergenic	40.0%
!!!	GAGACATTCGTTTTTTCTGC+AGG	+	11149:67384-67403	MS.gene062172:intergenic	40.0%
	AAAGGGATAGGAGATTGTGG+GGG	+	11149:66982-67001	MS.gene062171:intergenic	45.0%
	GAAAGGGATAGGAGATTGTG+GGG	+	11149:66983-67002	MS.gene062172:intergenic	45.0%
	GAGAAAGGGATAGGAGATTG+TGG	+	11149:66985-67004	MS.gene062172:intergenic	45.0%
	GTTTATGGGTGCTCTATCTG+TGG	-	11149:67240-67259	MS.gene062172:CDS	45.0%
!	GATAGAGCACCCATAAACGT+TGG	+	11149:67238-67257	MS.gene062172:intergenic	45.0%
!	GCAATGATGGAGAAAGGGAT+AGG	+	11149:66994-67013	MS.gene062172:intergenic	45.0%
!	TTGTCTGGTTCCAAAGCCAT+TGG	+	11149:67345-67364	MS.gene062172:intergenic	45.0%
!!	GTTGTTGTTGTTGGTAGTGG+TGG	+	11149:67202-67221	MS.gene062172:intergenic	45.0%
	AAGGGATAGGAGATTGTGGG+GGG	+	11149:66981-67000	MS.gene062172:intergenic	50.0%
	GGCGACCTCATTAACACCAA+TGG	-	11149:67326-67345	MS.gene062172:CDS	50.0%
	GTGCAGGGTGGAAGTTCATA+TGG	+	11149:67299-67318	MS.gene062172:intergenic	50.0%
	TATGAACTTCCACCCTGCAC+CGG	-	11149:67299-67318	MS.gene062172:CDS	50.0%
!!	GTTGTTGTTGGTAGTGGTGG+TGG	+	11149:67199-67218	MS.gene062172:intergenic	50.0%
	GTTAATGAGGTCGCCTCCAC+CGG	+	11149:67321-67340	MS.gene062172:intergenic	55.0%
	TCATATGGCCACCAAGTGCC+TGG	+	11149:67284-67303	MS.gene062172:intergenic	55.0%
	TTCACATCTGGCCAGGCACT+TGG	-	11149:67270-67289	MS.gene062172:CDS	55.0%
!!	GTTGTTGGTAGTGGTGGTGG+AGG	+	11149:67196-67215	MS.gene062172:intergenic	55.0%
	ACATCTGGCCAGGCACTTGG+TGG	-	11149:67273-67292	MS.gene062172:CDS	60.0%
!!	AGTGGTGGTGGAGGTGGTGT+TGG	+	11149:67187-67206	MS.gene062172:intergenic	60.0%
!!	GTTGGTAGTGGTGGTGGAGG+TGG	+	11149:67193-67212	MS.gene062172:intergenic	60.0%
	GAACTTCCACCCTGCACCGG+TGG	-	11149:67302-67321	MS.gene062172:CDS	65.0%
	AGGTCGCCTCCACCGGTGCA+GGG	+	11149:67314-67333	MS.gene062172:intergenic	70.0%
!	CTTCCACCCTGCACCGGTGG+AGG	-	11149:67305-67324	MS.gene062172:CDS	70.0%
	GAGGTCGCCTCCACCGGTGC+AGG	+	11149:67315-67334	MS.gene062172:intergenic	75.0%
	TCGCCTCCACCGGTGCAGGG+TGG	+	11149:67311-67330	MS.gene062172:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
11149	gene	66886	67437	66886	ID=MS.gene062172
11149	mRNA	66886	67437	66886	ID=MS.gene062172.t1;Parent=MS.gene062172
11149	exon	66886	67437	66886	ID=MS.gene062172.t1.exon1;Parent=MS.gene062172.t1
11149	CDS	66886	67437	66886	ID=cds.MS.gene062172.t1;Parent=MS.gene062172.t1

Gene Sequence

>MS.gene062172

ATGGAAGCATCTCACGAACAACAACTCTCACTAAACTCTACAGAACACACCATTAATGTCACCAAAGTGAATATAACTCAAAAGGTTATAAACCCCCCACAATCTCCTATCCCTTTCTCCATCATTGCTTATTCTTCTACTAGAGAAGGAGGAACAAGAACAAGAGAAAGTGCACGTTACAATGTGTATACATGCAAAACATGCAACAAAACATTCACTTCATTTCAAGCACTTGGAGGCCATAGAAAAGTTCACAAGAAGCCTAGAAAAGCTCATAGAAAAGGTCACAACAACAACACCAACACCACCTCCACCACCACTACCAACAACAACAACAACAACAACAAATCCAACGTTTATGGGTGCTCTATCTGTGGTTCTAAATTCACATCTGGCCAGGCACTTGGTGGCCATATGAACTTCCACCCTGCACCGGTGGAGGCGACCTCATTAACACCAATGGCTTTGGAACCAGACAAAGAAGATGAAGAACCACCTGCAGAAAAAACGAATGTCTCTTTGGATTTGGATCTCAACCTTCCTCCAGCTTAA

Protein sequence

>MS.gene062172.t1

MEASHEQQLSLNSTEHTINVTKVNITQKVINPPQSPIPFSIIAYSSTREGGTRTRESARYNVYTCKTCNKTFTSFQALGGHRKVHKKPRKAHRKGHNNNTNTTSTTTTNNNNNNNKSNVYGCSICGSKFTSGQALGGHMNFHPAPVEATSLTPMALEPDKEDEEPPAEKTNVSLDLDLNLPPA