AlfalfaGEDB | MS.gene062177

M. sativa cultivar XinJiangDaYe / MS.gene062177

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF13912.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062177.t1	XP_013468858.1	44.6	195	36	3	1	124	1	194	2.00E-23	118.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062177.t1	A0A072VXX0	44.6	195	36	3	1	124	1	194	1.5e-23	118.6

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene062177.t1	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062177.t1	MTR_1g077700	54.839	186	22	3	1	124	1	186	2.62e-46	148
MS.gene062177.t1	MTR_1g077730	89.474	76	6	2	1	75	1	75	4.19e-40	132
MS.gene062177.t1	MTR_1g077730	64.634	82	12	1	43	124	130	194	2.96e-30	107
MS.gene062177.t1	MTR_1g077120	85.185	27	4	0	37	63	40	66	3.39e-11	55.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene062177.t1	AT2G28200	60.526	38	15	0	37	74	108	145	5.73e-11	58.5

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Find 28 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAAATGAATGTCTCTT+TGG	0.298164	1.1:+58237195	MS.gene062177:CDS
AATAAGCAATGATGGAGAAA+GGG	0.497448	1.1:-58236727	None:intergenic
AATGAATGTCTCTTTGGATT+TGG	0.240224	1.1:+58237201	MS.gene062177:CDS
ACATCTGGCCAGGCACTTGG+TGG	0.529943	1.1:+58237082	MS.gene062177:intron
ACTTCATTTCAAGCACTTGG+AGG	0.520190	1.1:+58236827	MS.gene062177:CDS
AGGTTGCCTCCACCGGTGCA+TGG	0.568155	1.1:-58237120	None:intergenic
ATAAGCAATGATGGAGAAAG+GGG	0.560403	1.1:-58236726	None:intergenic
CAAAGCCATTGGTCTCGATG+AGG	0.605389	1.1:-58237140	None:intergenic
CATGAACAACAACTCTCTTT+AGG	0.379285	1.1:+58236680	MS.gene062177:CDS
CTCCACCGGTGCATGGTGGA+AGG	0.537215	1.1:-58237113	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAACAAAACCAAGGTTTA+TGG	+	chr1.1:58237034-58237053	MS.gene062177:intron	30.0%
AATGAATGTCTCTTTGGATT+TGG	+	chr1.1:58237201-58237220	MS.gene062177:CDS	30.0%
ACAACAAAACCAAGGTTTAT+GGG	+	chr1.1:58237035-58237054	MS.gene062177:intron	30.0%
TATTCTTCTACAAGAGAAGA+AGG	+	chr1.1:58236746-58236765	MS.gene062177:CDS	30.0%
TGTAGAAGAATAAGCAATGA+TGG	-	chr1.1:58236738-58236757	None:intergenic	30.0%
CAACAACAACAACAAAACCA+AGG	+	chr1.1:58237027-58237046	MS.gene062177:intron	35.0%
CATGAACAACAACTCTCTTT+AGG	+	chr1.1:58236680-58236699	MS.gene062177:CDS	35.0%
TTCACTTCATTTCAAGCACT+TGG	+	chr1.1:58236824-58236843	MS.gene062177:CDS	35.0%
ACTTCATTTCAAGCACTTGG+AGG	+	chr1.1:58236827-58236846	MS.gene062177:CDS	40.0%
GAAACCTAGAGATGATAGTC+AGG	+	chr1.1:58236868-58236887	MS.gene062177:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	58236668	58237246	58236668	ID=MS.gene062177
chr1.1	mRNA	58236668	58237246	58236668	ID=MS.gene062177.t1;Parent=MS.gene062177
chr1.1	exon	58236668	58236889	58236668	ID=MS.gene062177.t1.exon1;Parent=MS.gene062177.t1
chr1.1	CDS	58236668	58236889	58236668	ID=cds.MS.gene062177.t1;Parent=MS.gene062177.t1
chr1.1	exon	58237094	58237246	58237094	ID=MS.gene062177.t1.exon2;Parent=MS.gene062177.t1
chr1.1	CDS	58237094	58237246	58237094	ID=cds.MS.gene062177.t1;Parent=MS.gene062177.t1

Gene Sequence

>MS.gene062177

ATGGAAGCATCTCATGAACAACAACTCTCTTTAGGCTCTATAGAACAACAATCTCCTACCCCTTTCTCCATCATTGCTTATTCTTCTACAAGAGAAGAAGGAACAAGAGAAAGTGCACGTTACAATGTGTATGCATGCAAAACATGCAAGAAAACTTTCACTTCATTTCAAGCACTTGGAGGCCATAGAAAAGTTCACAAGAAACCTAGAGATGATAGTCAGGTTCAAGAGTTTCAATTCAAAATCAATAATAAATCAATTTCCCTTAAATTGAATAGTATCAATGGTATGGGAAATATATATGCTAGTAGCAACAACAACAACACCACCACCACTACCAACAACAACAATAACACCACCAACAACAACAACAAAACCAAGGTTTATGGGTGCACCATTTGTGGTTCTAAATTCACATCTGGCCAGGCACTTGGTGGCCATATGACCTTCCACCATGCACCGGTGGAGGCAACCTCATCGAGACCAATGGCTTTGAAACCAGACGAAGAAGATGAAGAACCACCCACAAAGAAAATGAATGTCTCTTTGGATTTGGATCTCAACGTTCCTGCAGCTTAA

Protein sequence

>MS.gene062177.t1

MEASHEQQLSLGSIEQQSPTPFSIIAYSSTREEGTRESARYNVYACKTCKKTFTSFQALGGHRKVHKKPRDDSQALGGHMTFHHAPVEATSSRPMALKPDEEDEEPPTKKMNVSLDLDLNVPAA