AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062277

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062277.t1 MTR_5g093010 97.872 141 3 0 17 157 5 145 3.22e-103 292
MS.gene062277.t1 MTR_7g028905 83.186 113 19 0 17 129 5 117 4.32e-69 204
MS.gene062277.t1 MTR_5g083230 51.376 109 53 0 18 126 7 115 3.41e-38 128
MS.gene062277.t1 MTR_3g031660 45.833 120 62 1 18 137 8 124 8.62e-37 125
MS.gene062277.t1 MTR_2g068760 48.571 105 54 0 18 122 14 118 9.38e-37 124
MS.gene062277.t1 MTR_3g071590 42.143 140 73 2 18 157 9 140 1.36e-36 124
MS.gene062277.t1 MTR_4g083680 52.525 99 47 0 18 116 16 114 3.31e-35 120
MS.gene062277.t1 MTR_5g083960 45.669 127 66 1 18 141 40 166 2.10e-33 117
MS.gene062277.t1 MTR_3g094690 50.000 106 53 0 18 123 48 153 2.24e-32 115
MS.gene062277.t1 MTR_3g452660 49.074 108 55 0 18 125 8 115 2.84e-32 116
MS.gene062277.t1 MTR_3g073690 45.631 103 56 0 18 120 10 112 6.85e-32 113
MS.gene062277.t1 MTR_7g033800 45.000 120 62 1 14 129 8 127 1.75e-31 111
MS.gene062277.t1 MTR_4g105170 47.664 107 56 0 18 124 12 118 1.82e-31 111
MS.gene062277.t1 MTR_4g060950 44.860 107 58 1 18 123 25 131 3.01e-31 111
MS.gene062277.t1 MTR_2g093310 43.396 106 60 0 18 123 34 139 7.08e-31 110
MS.gene062277.t1 MTR_6g005070 48.000 100 52 0 18 117 10 109 1.51e-30 108
MS.gene062277.t1 MTR_6g005080 40.876 137 74 2 18 154 11 140 1.94e-30 107
MS.gene062277.t1 MTR_5g017950 45.631 103 56 0 18 120 9 111 2.21e-30 112
MS.gene062277.t1 MTR_3g094690 50.000 106 52 1 18 123 48 152 2.60e-30 110
MS.gene062277.t1 MTR_3g077240 49.000 100 51 0 18 117 11 110 3.00e-30 108
MS.gene062277.t1 MTR_6g011250 37.956 137 82 2 13 146 5 141 5.34e-29 105
MS.gene062277.t1 MTR_3g071420 44.444 99 55 0 13 111 10 108 8.53e-29 104
MS.gene062277.t1 MTR_1g095850 43.564 101 57 0 18 118 73 173 1.83e-28 105
MS.gene062277.t1 MTR_5g075020 48.077 104 54 0 18 121 44 147 1.83e-28 105
MS.gene062277.t1 MTR_5g075020 48.077 104 54 0 18 121 48 151 2.30e-28 105
MS.gene062277.t1 MTR_5g083010 41.509 106 62 0 13 118 7 112 3.15e-28 103
MS.gene062277.t1 MTR_8g040900 56.250 80 35 0 18 97 8 87 4.58e-28 103
MS.gene062277.t1 MTR_6g027710 44.340 106 58 1 18 122 41 146 2.18e-27 102
MS.gene062277.t1 MTR_1g070220 42.975 121 64 2 12 131 10 126 3.73e-27 101
MS.gene062277.t1 MTR_7g096530 45.098 102 55 1 18 118 15 116 6.85e-27 100
MS.gene062277.t1 MTR_6g011230 40.594 101 60 0 14 114 8 108 7.82e-27 100
MS.gene062277.t1 MTR_7g074990 44.554 101 55 1 18 117 21 121 3.80e-25 96.7
MS.gene062277.t1 MTR_8g036085 41.509 106 61 1 18 122 24 129 8.47e-25 95.9
MS.gene062277.t1 MTR_8g079660 39.604 101 61 0 18 118 9 109 1.68e-24 95.5
MS.gene062277.t1 MTR_7g096610 41.732 127 67 3 18 137 9 135 1.77e-24 94.7
MS.gene062277.t1 MTR_1g070205 37.857 140 78 4 18 156 13 144 9.43e-24 93.2
MS.gene062277.t1 MTR_8g036105 43.137 102 57 1 18 118 19 120 1.43e-23 92.0
MS.gene062277.t1 MTR_8g079580 42.574 101 57 1 18 117 9 109 1.55e-23 92.4
MS.gene062277.t1 MTR_5g080470 41.176 102 59 1 18 118 8 109 4.18e-23 90.5
MS.gene062277.t1 MTR_6g027700 35.821 134 73 2 15 135 5 138 9.61e-23 89.7
MS.gene062277.t1 MTR_7g075230 36.879 141 83 4 18 152 13 153 9.67e-23 89.7
MS.gene062277.t1 MTR_8g079620 36.634 101 64 0 18 118 9 109 4.37e-22 89.0
MS.gene062277.t1 MTR_6g011200 35.294 102 65 1 18 118 10 111 8.36e-22 88.2
MS.gene062277.t1 MTR_5g085390 33.333 144 87 4 18 156 10 149 2.46e-21 86.7
MS.gene062277.t1 MTR_4g107450 40.000 100 60 0 18 117 11 110 2.69e-20 85.1
MS.gene062277.t1 MTR_8g017090 37.624 101 63 0 18 118 13 113 7.21e-20 84.0
MS.gene062277.t1 MTR_4g099230 33.654 104 68 1 16 118 1 104 9.78e-20 80.9
MS.gene062277.t1 MTR_2g100020 33.663 101 67 0 18 118 20 120 1.03e-18 80.5
MS.gene062277.t1 MTR_4g088035 31.395 86 59 0 18 103 7 92 4.28e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062277.t1 AT3G26660 64.706 102 36 0 17 118 5 106 1.62e-45 145
MS.gene062277.t1 AT3G26620 64.706 102 36 0 17 118 5 106 1.83e-45 145
MS.gene062277.t1 AT2G30130 50.926 108 53 0 18 125 9 116 1.49e-37 127
MS.gene062277.t1 AT1G31320 60.465 86 34 0 18 103 14 99 6.36e-35 120
MS.gene062277.t1 AT5G63090 46.087 115 62 0 18 132 12 126 3.53e-32 113
MS.gene062277.t1 AT5G63090 46.087 115 62 0 18 132 12 126 3.53e-32 113
MS.gene062277.t1 AT5G63090 46.087 115 62 0 18 132 12 126 3.53e-32 113
MS.gene062277.t1 AT5G63090 46.087 115 62 0 18 132 12 126 3.53e-32 113
MS.gene062277.t1 AT3G27650 49.000 100 51 0 18 117 40 139 5.40e-32 112
MS.gene062277.t1 AT2G28500 44.340 106 59 0 18 123 56 161 2.47e-31 112
MS.gene062277.t1 AT1G07900 44.340 106 59 0 18 123 34 139 2.49e-31 111
MS.gene062277.t1 AT1G16530 49.505 101 50 1 18 117 15 115 3.00e-31 110
MS.gene062277.t1 AT2G30340 47.170 106 56 0 18 123 53 158 4.99e-31 112
MS.gene062277.t1 AT2G23660 44.860 107 59 0 14 120 2 108 5.22e-31 113
MS.gene062277.t1 AT2G23660 44.860 107 59 0 14 120 2 108 5.22e-31 113
MS.gene062277.t1 AT2G23660 44.860 107 59 0 14 120 2 108 5.22e-31 113
MS.gene062277.t1 AT2G30340 47.170 106 56 0 18 123 54 159 5.27e-31 112
MS.gene062277.t1 AT5G66870 44.444 108 60 0 13 120 3 110 6.98e-31 113
MS.gene062277.t1 AT2G40470 45.385 130 65 2 18 147 21 144 6.79e-30 108
MS.gene062277.t1 AT2G40470 45.385 130 65 2 18 147 46 169 9.84e-30 108
MS.gene062277.t1 AT3G11090 40.179 112 67 0 13 124 7 118 3.02e-29 105
MS.gene062277.t1 AT1G65620 53.750 80 37 0 18 97 10 89 9.79e-28 102
MS.gene062277.t1 AT1G65620 53.750 80 37 0 18 97 10 89 9.79e-28 102
MS.gene062277.t1 AT1G65620 53.750 80 37 0 18 97 10 89 9.79e-28 102
MS.gene062277.t1 AT1G65620 53.750 80 37 0 18 97 10 89 9.79e-28 102
MS.gene062277.t1 AT1G65620 53.750 80 37 0 18 97 10 89 9.79e-28 102
MS.gene062277.t1 AT4G00210 39.259 135 72 3 18 144 12 144 9.20e-27 100
MS.gene062277.t1 AT3G03760 45.545 101 54 1 18 117 52 152 9.42e-27 101
MS.gene062277.t1 AT3G50510 40.708 113 67 0 6 118 1 113 9.42e-27 100
MS.gene062277.t1 AT3G50510 40.708 113 67 0 6 118 1 113 9.42e-27 100
MS.gene062277.t1 AT2G42430 43.810 105 58 1 18 121 16 120 3.47e-26 99.8
MS.gene062277.t1 AT5G06080 41.322 121 67 2 18 137 8 125 2.83e-25 95.5
MS.gene062277.t1 AT2G45410 45.000 100 54 1 20 118 19 118 1.62e-24 94.0
MS.gene062277.t1 AT4G00220 40.385 104 61 1 20 122 20 123 1.10e-23 92.8
MS.gene062277.t1 AT3G58190 43.137 102 57 1 18 118 12 113 1.36e-23 92.4
MS.gene062277.t1 AT2G42440 43.000 100 56 1 18 116 8 107 1.44e-23 92.8
MS.gene062277.t1 AT2G45420 42.308 104 59 1 20 122 40 143 7.16e-23 91.3
MS.gene062277.t1 AT3G13850 33.766 154 82 2 18 151 37 190 1.71e-22 90.5
MS.gene062277.t1 AT3G47870 37.037 108 68 0 14 121 33 140 2.23e-22 91.3
MS.gene062277.t1 AT5G35900 40.777 103 54 1 18 113 6 108 2.40e-20 83.6
MS.gene062277.t1 AT2G31310 31.206 141 89 3 18 157 8 141 5.78e-20 82.0
MS.gene062277.t1 AT1G72980 32.836 134 86 2 14 146 10 140 6.69e-18 77.4
MS.gene062277.t1 AT1G06280 37.209 86 54 0 18 103 25 110 2.11e-17 75.9
MS.gene062277.t1 AT2G19820 34.021 97 58 2 18 113 13 104 6.55e-15 67.4
MS.gene062277.t1 AT4G22700 31.858 113 75 2 14 125 2 113 4.56e-13 63.9
MS.gene062277.t1 AT5G15060 43.243 74 38 2 18 88 18 90 1.51e-12 61.6
MS.gene062277.t1 AT1G36000 27.957 93 67 0 18 110 10 102 1.19e-11 58.9

Find 35 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTCATATTGTTGATTT+TGG 0.095309 5.1:+74140507 None:intergenic
AGGATTCAAAATCCTGTTTA+TGG 0.168254 5.1:-74140623 MS.gene062277:CDS
AGCAAATCTTTGAGGATTAT+TGG 0.230583 5.1:+74140885 None:intergenic
AGCAATCTGAGTTTGGATTT+TGG 0.249357 5.1:+74140541 None:intergenic
GGATTATTGGCAGGGAAATA+TGG 0.290635 5.1:+74140898 None:intergenic
GGCCCAATCAAACACCTAAT+TGG 0.314557 5.1:-74140430 MS.gene062277:CDS
GCTTCTGTCCACCGAATCTA+TGG 0.338967 5.1:-74140865 MS.gene062277:CDS
TATGACAAGCAATCTGAGTT+TGG 0.340516 5.1:+74140534 None:intergenic
AAGTCTTAATTGAACCAATT+AGG 0.341976 5.1:+74140416 None:intergenic
GAACCAATTAGGTGTTTGAT+TGG 0.385252 5.1:+74140427 None:intergenic
CCAGCTCAGCTTCTGTATCA+TGG 0.391779 5.1:+74140564 None:intergenic
ATCCCAACACATCCATAAAC+AGG 0.441067 5.1:+74140611 None:intergenic
AATCTTTGAGGATTATTGGC+AGG 0.455276 5.1:+74140889 None:intergenic
TGCTCCATGCTGCTACTTTG+TGG 0.458851 5.1:+74140461 None:intergenic
AACCAATTAGGTGTTTGATT+GGG 0.459934 5.1:+74140428 None:intergenic
CCATGATACAGAAGCTGAGC+TGG 0.490473 5.1:-74140564 MS.gene062277:CDS
TGTATTTAGAAGCACAATGT+AGG 0.494601 5.1:-74140643 MS.gene062277:CDS
TGGACAGAAGCAAATCTTTG+AGG 0.503992 5.1:+74140877 None:intergenic
CAAAATCAACAATATGAAGC+TGG 0.506425 5.1:-74140506 MS.gene062277:CDS
ATCTATGGTGGTGCCAATGT+TGG 0.519520 5.1:-74140850 MS.gene062277:CDS
CATTGGCACCACCATAGATT+CGG 0.530883 5.1:+74140854 None:intergenic
AATCCTGTTTATGGATGTGT+TGG 0.538555 5.1:-74140614 MS.gene062277:CDS
GGAGAGAAAATGCAATCAGA+AGG 0.551169 5.1:+74140919 None:intergenic
CTTGCAAGAGTCAAAGAAGA+AGG 0.555730 5.1:-74140945 MS.gene062277:CDS
ATCTTTGAGGATTATTGGCA+GGG 0.557843 5.1:+74140890 None:intergenic
AGTGTCGGAAGTCTTCATTA+AGG 0.572771 5.1:-74141904 MS.gene062277:intron
TGCTCTTCCTCGTACAGTGT+CGG 0.575095 5.1:-74141919 MS.gene062277:CDS
GCCTCCACAAAGTAGCAGCA+TGG 0.580606 5.1:-74140465 MS.gene062277:CDS
ATCCTGTTTATGGATGTGTT+GGG 0.594258 5.1:-74140613 MS.gene062277:CDS
GCTTGTTCTCTTACATAGTG+AGG 0.601870 5.1:+74140677 None:intergenic
GAGAGAAAATGCAATCAGAA+GGG 0.619697 5.1:+74140920 None:intergenic
TGGCACCACCATAGATTCGG+TGG 0.632816 5.1:+74140857 None:intergenic
TCTGTCCACCGAATCTATGG+TGG 0.637607 5.1:-74140862 MS.gene062277:CDS
TCCATGCTGCTACTTTGTGG+AGG 0.665689 5.1:+74140464 None:intergenic
AAGACTTCCGACACTGTACG+AGG 0.715668 5.1:+74141912 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAAGTTAAATAAAAAATAA+AGG - chr5.1:74140629-74140648 MS.gene062277:CDS 10.0%
!! TGTAATATAATTATAACATA+TGG - chr5.1:74141585-74141604 MS.gene062277:intron 10.0%
!! AAAAACAATAATTCTTTCTT+TGG - chr5.1:74141304-74141323 MS.gene062277:intron 15.0%
!! AAATTAAGAAAACAATTATC+TGG - chr5.1:74140754-74140773 MS.gene062277:intron 15.0%
!! AATATGATTGTATGAAAAAT+TGG + chr5.1:74141002-74141021 None:intergenic 15.0%
!! AATTAAGAAAACAATTATCT+GGG - chr5.1:74140755-74140774 MS.gene062277:intron 15.0%
!! AATTAATTGAAAAATAGTGA+TGG - chr5.1:74140479-74140498 MS.gene062277:CDS 15.0%
!! GTTAAATAAAAAATAAAGGA+TGG - chr5.1:74140633-74140652 MS.gene062277:CDS 15.0%
!!! CAATAATAATAACTTGTAAA+TGG - chr5.1:74140957-74140976 MS.gene062277:intron 15.0%
!!! CTTTTAATTTTTTTCTTTGA+TGG - chr5.1:74140538-74140557 MS.gene062277:CDS 15.0%
!!! TTCTTATCTAGTATTTTTAT+TGG - chr5.1:74141642-74141661 MS.gene062277:intron 15.0%
!! AAAATTGTGAAATTCATAGA+GGG + chr5.1:74141142-74141161 None:intergenic 20.0%
!! TAAAATTGTGAAATTCATAG+AGG + chr5.1:74141143-74141162 None:intergenic 20.0%
!! TTAGAAATTAGAACTAGAAA+AGG + chr5.1:74141087-74141106 None:intergenic 20.0%
!! TTTGAACTAGTATAAACTAA+AGG + chr5.1:74140834-74140853 None:intergenic 20.0%
!!! TTTTTTTCCTCTTTATACAA+AGG - chr5.1:74141347-74141366 MS.gene062277:intron 20.0%
! AAACTGAACAAATTGAATCA+TGG + chr5.1:74140518-74140537 None:intergenic 25.0%
! AAATTGTGAAATTCATAGAG+GGG + chr5.1:74141141-74141160 None:intergenic 25.0%
! AAATTTATAAATGTCAACGC+AGG - chr5.1:74141048-74141067 MS.gene062277:intron 25.0%
! ATAATCCATGTGAAAAATAG+TGG + chr5.1:74140579-74140598 None:intergenic 25.0%
! ATCAAATCCTTTGTATAAAG+AGG + chr5.1:74141357-74141376 None:intergenic 25.0%
! CAATACAAATACTATTCTCT+TGG - chr5.1:74141233-74141252 MS.gene062277:intron 25.0%
! GTATTTGTATTGTAGATACT+AGG + chr5.1:74141225-74141244 None:intergenic 25.0%
! GTTTATTAAGGAAATCCTAA+CGG + chr5.1:74141203-74141222 None:intergenic 25.0%
! TGTAGATACTAGGTTTATTA+AGG + chr5.1:74141215-74141234 None:intergenic 25.0%
! TTCAATTAAACAAGTTTCTC+TGG - chr5.1:74140661-74140680 MS.gene062277:CDS 25.0%
!! AATTTAGACTTTTCTCTCTA+TGG - chr5.1:74140685-74140704 MS.gene062277:CDS 25.0%
!!! GATTTTGACATCATGATTTA+TGG - chr5.1:74141374-74141393 MS.gene062277:intron 25.0%
AACCAATTAGGTGTTTGATT+GGG + chr5.1:74141928-74141947 None:intergenic 30.0%
AGGATTCAAAATCCTGTTTA+TGG - chr5.1:74141730-74141749 MS.gene062277:intron 30.0%
CAAAATCAACAATATGAAGC+TGG - chr5.1:74141847-74141866 MS.gene062277:intron 30.0%
CTATATTTGCAATGCTAAGA+AGG + chr5.1:74141180-74141199 None:intergenic 30.0%
TCTTTATTCTCTACTGTGTT+GGG + chr5.1:74140722-74140741 None:intergenic 30.0%
TTGACATCATGATTTATGGT+AGG - chr5.1:74141378-74141397 MS.gene062277:intron 30.0%
! AAACAATTATCTGGGACAAA+AGG - chr5.1:74140763-74140782 MS.gene062277:intron 30.0%
! AGCAAATCTTTGAGGATTAT+TGG + chr5.1:74141471-74141490 None:intergenic 30.0%
! TGTATTTAGAAGCACAATGT+AGG - chr5.1:74141710-74141729 MS.gene062277:intron 30.0%
!!! CAGCTTCATATTGTTGATTT+TGG + chr5.1:74141849-74141868 None:intergenic 30.0%
AATCCTGTTTATGGATGTGT+TGG - chr5.1:74141739-74141758 MS.gene062277:intron 35.0%
ACCCTTTAGTGTGTATAAGT+GGG + chr5.1:74140902-74140921 None:intergenic 35.0%
ATAGTCATTGCGATATGCAA+TGG - chr5.1:74140854-74140873 MS.gene062277:CDS 35.0%
ATCCATGTGAAAAATAGTGG+TGG + chr5.1:74140576-74140595 None:intergenic 35.0%
ATCCTGTTTATGGATGTGTT+GGG - chr5.1:74141740-74141759 MS.gene062277:intron 35.0%
CAATGTTGGAAAAATGCTTC+AGG - chr5.1:74141517-74141536 MS.gene062277:intron 35.0%
GAACCAATTAGGTGTTTGAT+TGG + chr5.1:74141929-74141948 None:intergenic 35.0%
GAGAGAAAATGCAATCAGAA+GGG + chr5.1:74141436-74141455 None:intergenic 35.0%
GTCTTTATTCTCTACTGTGT+TGG + chr5.1:74140723-74140742 None:intergenic 35.0%
TATGACAAGCAATCTGAGTT+TGG + chr5.1:74141822-74141841 None:intergenic 35.0%
! AATCTTTGAGGATTATTGGC+AGG + chr5.1:74141467-74141486 None:intergenic 35.0%
! AGCAATCTGAGTTTGGATTT+TGG + chr5.1:74141815-74141834 None:intergenic 35.0%
! ATCTTTGAGGATTATTGGCA+GGG + chr5.1:74141466-74141485 None:intergenic 35.0%
!! CTGAAGCATTTTTCCAACAT+TGG + chr5.1:74141519-74141538 None:intergenic 35.0%
ATCCCAACACATCCATAAAC+AGG + chr5.1:74141745-74141764 None:intergenic 40.0%
CACCCTTTAGTGTGTATAAG+TGG + chr5.1:74140903-74140922 None:intergenic 40.0%
CCCCACTTATACACACTAAA+GGG - chr5.1:74140898-74140917 MS.gene062277:CDS 40.0%
CCCTTTAGTGTGTATAAGTG+GGG + chr5.1:74140901-74140920 None:intergenic 40.0%
CTTGCAAGAGTCAAAGAAGA+AGG - chr5.1:74141408-74141427 MS.gene062277:intron 40.0%
GCATTGCAAATATAGCCGTT+AGG - chr5.1:74141185-74141204 MS.gene062277:intron 40.0%
GCTTGTTCTCTTACATAGTG+AGG + chr5.1:74141679-74141698 None:intergenic 40.0%
GGAGAGAAAATGCAATCAGA+AGG + chr5.1:74141437-74141456 None:intergenic 40.0%
GGATTATTGGCAGGGAAATA+TGG + chr5.1:74141458-74141477 None:intergenic 40.0%
TCATTGCGATATGCAATGGT+TGG - chr5.1:74140858-74140877 MS.gene062277:CDS 40.0%
TCCCCACTTATACACACTAA+AGG - chr5.1:74140897-74140916 MS.gene062277:CDS 40.0%
TGGACAGAAGCAAATCTTTG+AGG + chr5.1:74141479-74141498 None:intergenic 40.0%
TTTCTCTCTATGGCAAAACG+AGG - chr5.1:74140695-74140714 MS.gene062277:intron 40.0%
! AGTGTCGGAAGTCTTCATTA+AGG - chr5.1:74140449-74140468 MS.gene062277:CDS 40.0%
!! TGCCACCACTATTTTTCACA+TGG - chr5.1:74140571-74140590 MS.gene062277:CDS 40.0%
CAATGGTTGGAGTTTCAACC+TGG - chr5.1:74140871-74140890 MS.gene062277:CDS 45.0%
CATTGGCACCACCATAGATT+CGG + chr5.1:74141502-74141521 None:intergenic 45.0%
GGCCCAATCAAACACCTAAT+TGG - chr5.1:74141923-74141942 MS.gene062277:CDS 45.0%
GTGTATAAGTGGGGAATTCC+AGG + chr5.1:74140892-74140911 None:intergenic 45.0%
! ATCTATGGTGGTGCCAATGT+TGG - chr5.1:74141503-74141522 MS.gene062277:intron 45.0%
!! GCAGCATGGAGCATTTTCAA+TGG - chr5.1:74141902-74141921 MS.gene062277:intron 45.0%
!! TCATAGAGGGGCATTGATCA+AGG + chr5.1:74141129-74141148 None:intergenic 45.0%
AAGACTTCCGACACTGTACG+AGG + chr5.1:74140444-74140463 None:intergenic 50.0%
CCAGCTCAGCTTCTGTATCA+TGG + chr5.1:74141792-74141811 None:intergenic 50.0%
CCATGATACAGAAGCTGAGC+TGG - chr5.1:74141789-74141808 MS.gene062277:intron 50.0%
GCTTCTGTCCACCGAATCTA+TGG - chr5.1:74141488-74141507 MS.gene062277:intron 50.0%
TCTGTCCACCGAATCTATGG+TGG - chr5.1:74141491-74141510 MS.gene062277:intron 50.0%
TGCTCTTCCTCGTACAGTGT+CGG - chr5.1:74140434-74140453 MS.gene062277:CDS 50.0%
!! TCCATGCTGCTACTTTGTGG+AGG + chr5.1:74141892-74141911 None:intergenic 50.0%
!! TGCTCCATGCTGCTACTTTG+TGG + chr5.1:74141895-74141914 None:intergenic 50.0%
GCCTCCACAAAGTAGCAGCA+TGG - chr5.1:74141888-74141907 MS.gene062277:intron 55.0%
TGGCACCACCATAGATTCGG+TGG + chr5.1:74141499-74141518 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 74140421 74141954 74140421 ID=MS.gene062277
chr5.1 mRNA 74140421 74141954 74140421 ID=MS.gene062277.t1;Parent=MS.gene062277
chr5.1 exon 74141905 74141954 74141905 ID=MS.gene062277.t1.exon1;Parent=MS.gene062277.t1
chr5.1 CDS 74141905 74141954 74141905 ID=cds.MS.gene062277.t1;Parent=MS.gene062277.t1
chr5.1 exon 74140837 74140975 74140837 ID=MS.gene062277.t1.exon2;Parent=MS.gene062277.t1
chr5.1 CDS 74140837 74140975 74140837 ID=cds.MS.gene062277.t1;Parent=MS.gene062277.t1
chr5.1 exon 74140421 74140705 74140421 ID=MS.gene062277.t1.exon3;Parent=MS.gene062277.t1
chr5.1 CDS 74140421 74140705 74140421 ID=cds.MS.gene062277.t1;Parent=MS.gene062277.t1
Gene Sequence

>MS.gene062277

ATGGAAATGCTGATGCTCTTCCTCGTACAGTGTCGGAAGTCTTCATTAAGGTATAATTAATTAATTGAAAAATAGTGATGGCATCAAAATATCACCATGATTCAATTTGTTCAGTTTCTTTTAATTTTTTTCTTTGATGGACTTTATTTCTGCCACCACTATTTTTCACATGGATTATGTTTATGTCATTATATAGATGCAGATTTAGTCAAGTTAAATAAAAAATAAAGGATGGTTGATTTCAATTAAACAAGTTTCTCTGGAAATTTAGACTTTTCTCTCTATGGCAAAACGAGGACCCAACACAGTAGAGAATAAAGACAACGTTTGCAAAAATTAAGAAAACAATTATCTGGGACAAAAGGAAAAAAAATAGATATTTATGTTTAAAGATAAATTTACACAACTAACCTTTAGTTTATACTAGTTCAAAATAGTCATTGCGATATGCAATGGTTGGAGTTTCAACCTGGAATTCCCCACTTATACACACTAAAGGGTGAATGTCACATTGAACTGTTTGACAAAAAAAAAATCAATAATAATAACTTGTAAATGGACACTCAATATTTTAATTTCCAATTTTTCATACAATCATATTTCTCTTTTAATTACGTCTTTATTCTTAAATTTATAAATGTCAACGCAGGTATTTATTTACTTCCTTTTCTAGTTCTAATTTCTAATACTAAATCATGCATATTGCCTTGATCAATGCCCCTCTATGAATTTCACAATTTTAATGTACAAAACTTTCCTTCTTAGCATTGCAAATATAGCCGTTAGGATTTCCTTAATAAACCTAGTATCTACAATACAAATACTATTCTCTTGGCACATTATTCATCAGATTTTGTGCAAGATTACTAAGATCACAAATTTAAAAAACAATAATTCTTTCTTTGGTGTCAGATTTTGTATTTGAATTTTTTTCCTCTTTATACAAAGGATTTGATTTTGACATCATGATTTATGGTAGGTGTGCTGCTTGCAAGAGTCAAAGAAGAAGGTGCCCTTCTGATTGCATTTTCTCTCCATATTTCCCTGCCAATAATCCTCAAAGATTTGCTTCTGTCCACCGAATCTATGGTGGTGCCAATGTTGGAAAAATGCTTCAGGTACTTTTAATAATGTATTGATATTTAGTATCTAGTATTTATATTATGTAATATAATTATAACATATGGAAAGAATATCAAAGTTATAAAAAATATATGTTTTTTCTTATCTAGTATTTTTATTGGTGCAGCAATTGCCTCACTATGTAAGAGAACAAGCTGCAAATACTTTGTATTTAGAAGCACAATGTAGGATTCAAAATCCTGTTTATGGATGTGTTGGGATCATCTCTAAATTGTATCAACAAATCCATGATACAGAAGCTGAGCTGGCCAAAATCCAAACTCAGATTGCTTGTCATAAGCTCCAAAATCAACAATATGAAGCTGGATCAAACTTCAATCTTTTGCCTCCACAAAGTAGCAGCATGGAGCATTTTCAATGGCCCAATCAAACACCTAATTGGTTCAATTAA
Protein sequence

>MS.gene062277.t1

MEMLMLFLVQCRKSSLRCAACKSQRRRCPSDCIFSPYFPANNPQRFASVHRIYGGANVGKMLQQLPHYVREQAANTLYLEAQCRIQNPVYGCVGIISKLYQQIHDTEAELAKIQTQIACHKLQNQQYEAGSNFNLLPPQSSSMEHFQWPNQTPNWFN