Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062441.t1 | XP_024630495.1 | 83.2 | 137 | 23 | 0 | 8 | 144 | 995 | 1131 | 1.30E-61 | 245.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062441.t1 | G7JX45 | 59.5 | 84 | 32 | 1 | 62 | 143 | 25 | 108 | 5.1e-20 | 107.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene062441.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062441.t1 | MTR_5g053510 | 58.824 | 85 | 33 | 1 | 62 | 144 | 25 | 109 | 3.82e-29 | 102 |
| MS.gene062441.t1 | MTR_1g054495 | 29.197 | 137 | 93 | 3 | 4 | 140 | 87 | 219 | 6.29e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCGATTTCAATGATTTCTTA+TGG | 0.230421 | 7.3:-75531463 | MS.gene062441:CDS |
| CCAACCCTTCGTTAGCTTGC+TGG | 0.293858 | 7.3:+75531352 | None:intergenic |
| CCATAAGAAATCATTGAAAT+CGG | 0.346978 | 7.3:+75531463 | None:intergenic |
| GCACATCTCCAGCCTGATAT+TGG | 0.349705 | 7.3:+75531309 | None:intergenic |
| CACATTGACCCCTTTGCAAA+TGG | 0.355395 | 7.3:-75531622 | MS.gene062441:CDS |
| CTGCACAACCAGCCAATATC+AGG | 0.358399 | 7.3:-75531321 | MS.gene062441:CDS |
| CTGCGTTTCAAGTTCTTTGA+TGG | 0.370866 | 7.3:-75531287 | MS.gene062441:CDS |
| GACTATTTGACCATTGTCAT+TGG | 0.378913 | 7.3:+75531412 | None:intergenic |
| CTGATTCTGTTTAGCTATGT+TGG | 0.402336 | 7.3:-75531759 | MS.gene062441:CDS |
| TTCAATGATTTCTTATGGAA+TGG | 0.456862 | 7.3:-75531458 | MS.gene062441:CDS |
| AATGGATGCGAATTCTGAAT+TGG | 0.461274 | 7.3:-75531604 | MS.gene062441:intron |
| GAAGTCTTGTGCTTCTGCTA+TGG | 0.476473 | 7.3:+75531673 | None:intergenic |
| AGTAGATTTGTTATCACACT+TGG | 0.481808 | 7.3:-75531732 | MS.gene062441:CDS |
| ATCTCCAGCCTGATATTGGC+TGG | 0.482605 | 7.3:+75531313 | None:intergenic |
| AATTCGCATCCATTTGCAAA+GGG | 0.485601 | 7.3:+75531613 | None:intergenic |
| CATAAGAAATCATTGAAATC+GGG | 0.494072 | 7.3:+75531464 | None:intergenic |
| CAGCAAGCTAACGAAGGGTT+GGG | 0.497952 | 7.3:-75531351 | MS.gene062441:CDS |
| CACACTTGGGAATACAATTG+AGG | 0.504352 | 7.3:-75531718 | MS.gene062441:CDS |
| CCAGCAAGCTAACGAAGGGT+TGG | 0.517788 | 7.3:-75531352 | MS.gene062441:CDS |
| TGCGTTTCAAGTTCTTTGAT+GGG | 0.523138 | 7.3:-75531286 | MS.gene062441:CDS |
| ACTGCTATTCATTACCAAGT+TGG | 0.542667 | 7.3:-75531386 | MS.gene062441:CDS |
| AATCATTGAAATCGGGTGGA+AGG | 0.560043 | 7.3:+75531471 | None:intergenic |
| GAATTCGCATCCATTTGCAA+AGG | 0.571229 | 7.3:+75531612 | None:intergenic |
| GTAGATTTGTTATCACACTT+GGG | 0.584740 | 7.3:-75531731 | MS.gene062441:CDS |
| TTGTTCCTCTCCAATGACAA+TGG | 0.585814 | 7.3:-75531422 | MS.gene062441:CDS |
| AAGAAATCATTGAAATCGGG+TGG | 0.589665 | 7.3:+75531467 | None:intergenic |
| TTTGACCATTGTCATTGGAG+AGG | 0.609768 | 7.3:+75531417 | None:intergenic |
| ACTCACCAGCAAGCTAACGA+AGG | 0.624585 | 7.3:-75531357 | MS.gene062441:CDS |
| GTGCTTCTGCTATGGTACGT+AGG | 0.636405 | 7.3:+75531681 | None:intergenic |
| CAAGCTAACGAAGGGTTGGG+AGG | 0.643100 | 7.3:-75531348 | MS.gene062441:CDS |
| ACAACCAGCCAATATCAGGC+TGG | 0.644275 | 7.3:-75531317 | MS.gene062441:CDS |
| CTCACCAGCAAGCTAACGAA+GGG | 0.650798 | 7.3:-75531356 | MS.gene062441:CDS |
| ATTCGCATCCATTTGCAAAG+GGG | 0.653436 | 7.3:+75531614 | None:intergenic |
| TGGTGAGTGAGTCACCAACT+TGG | 0.680717 | 7.3:+75531372 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAACAATGAAAATTAAGATT+AGG | + | chr7.3:75531505-75531524 | None:intergenic | 20.0% |
| !! | TGTTTATTAAAATCTGCAAA+TGG | - | chr7.3:75531455-75531474 | MS.gene062441:CDS | 20.0% |
| ! | CATAAGAAATCATTGAAATC+GGG | + | chr7.3:75531548-75531567 | None:intergenic | 25.0% |
| ! | CCATAAGAAATCATTGAAAT+CGG | + | chr7.3:75531549-75531568 | None:intergenic | 25.0% |
| ! | TTCAATGATTTCTTATGGAA+TGG | - | chr7.3:75531551-75531570 | MS.gene062441:intron | 25.0% |
| !!! | TGCAGATTTTAATAAACACA+TGG | + | chr7.3:75531453-75531472 | None:intergenic | 25.0% |
| AGATTAGGTGTAAAAAGAGT+TGG | + | chr7.3:75531490-75531509 | None:intergenic | 30.0% | |
| CCGATTTCAATGATTTCTTA+TGG | - | chr7.3:75531546-75531565 | MS.gene062441:intron | 30.0% | |
| TTTCATTGTTGTCTAAATGC+AGG | - | chr7.3:75531514-75531533 | MS.gene062441:intron | 30.0% | |
| !! | AGTAGATTTGTTATCACACT+TGG | - | chr7.3:75531277-75531296 | MS.gene062441:CDS | 30.0% |
| !! | GTAGATTTGTTATCACACTT+GGG | - | chr7.3:75531278-75531297 | MS.gene062441:CDS | 30.0% |
| AAGAAATCATTGAAATCGGG+TGG | + | chr7.3:75531545-75531564 | None:intergenic | 35.0% | |
| AATGGATGCGAATTCTGAAT+TGG | - | chr7.3:75531405-75531424 | MS.gene062441:CDS | 35.0% | |
| AATTCGCATCCATTTGCAAA+GGG | + | chr7.3:75531399-75531418 | None:intergenic | 35.0% | |
| ACTGCTATTCATTACCAAGT+TGG | - | chr7.3:75531623-75531642 | MS.gene062441:CDS | 35.0% | |
| CTGATTCTGTTTAGCTATGT+TGG | - | chr7.3:75531250-75531269 | MS.gene062441:CDS | 35.0% | |
| GACTATTTGACCATTGTCAT+TGG | + | chr7.3:75531600-75531619 | None:intergenic | 35.0% | |
| !! | TGCGTTTCAAGTTCTTTGAT+GGG | - | chr7.3:75531723-75531742 | MS.gene062441:CDS | 35.0% |
| AATCATTGAAATCGGGTGGA+AGG | + | chr7.3:75531541-75531560 | None:intergenic | 40.0% | |
| ATTCGCATCCATTTGCAAAG+GGG | + | chr7.3:75531398-75531417 | None:intergenic | 40.0% | |
| CACACTTGGGAATACAATTG+AGG | - | chr7.3:75531291-75531310 | MS.gene062441:CDS | 40.0% | |
| GAATTCGCATCCATTTGCAA+AGG | + | chr7.3:75531400-75531419 | None:intergenic | 40.0% | |
| TTGTTCCTCTCCAATGACAA+TGG | - | chr7.3:75531587-75531606 | MS.gene062441:intron | 40.0% | |
| TTTGACCATTGTCATTGGAG+AGG | + | chr7.3:75531595-75531614 | None:intergenic | 40.0% | |
| !! | CTGCGTTTCAAGTTCTTTGA+TGG | - | chr7.3:75531722-75531741 | MS.gene062441:CDS | 40.0% |
| CACATTGACCCCTTTGCAAA+TGG | - | chr7.3:75531387-75531406 | MS.gene062441:CDS | 45.0% | |
| ! | GAAGTCTTGTGCTTCTGCTA+TGG | + | chr7.3:75531339-75531358 | None:intergenic | 45.0% |
| ACAACCAGCCAATATCAGGC+TGG | - | chr7.3:75531692-75531711 | MS.gene062441:CDS | 50.0% | |
| ACTCACCAGCAAGCTAACGA+AGG | - | chr7.3:75531652-75531671 | MS.gene062441:CDS | 50.0% | |
| ATCTCCAGCCTGATATTGGC+TGG | + | chr7.3:75531699-75531718 | None:intergenic | 50.0% | |
| CAGCAAGCTAACGAAGGGTT+GGG | - | chr7.3:75531658-75531677 | MS.gene062441:CDS | 50.0% | |
| CTCACCAGCAAGCTAACGAA+GGG | - | chr7.3:75531653-75531672 | MS.gene062441:CDS | 50.0% | |
| CTGCACAACCAGCCAATATC+AGG | - | chr7.3:75531688-75531707 | MS.gene062441:CDS | 50.0% | |
| GCACATCTCCAGCCTGATAT+TGG | + | chr7.3:75531703-75531722 | None:intergenic | 50.0% | |
| GTGCTTCTGCTATGGTACGT+AGG | + | chr7.3:75531331-75531350 | None:intergenic | 50.0% | |
| TGGTGAGTGAGTCACCAACT+TGG | + | chr7.3:75531640-75531659 | None:intergenic | 50.0% | |
| CAAGCTAACGAAGGGTTGGG+AGG | - | chr7.3:75531661-75531680 | MS.gene062441:CDS | 55.0% | |
| CCAGCAAGCTAACGAAGGGT+TGG | - | chr7.3:75531657-75531676 | MS.gene062441:CDS | 55.0% | |
| !! | CCAACCCTTCGTTAGCTTGC+TGG | + | chr7.3:75531660-75531679 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 75531244 | 75531787 | 75531244 | ID=MS.gene062441 |
| chr7.3 | mRNA | 75531244 | 75531787 | 75531244 | ID=MS.gene062441.t1;Parent=MS.gene062441 |
| chr7.3 | exon | 75531605 | 75531787 | 75531605 | ID=MS.gene062441.t1.exon1;Parent=MS.gene062441.t1 |
| chr7.3 | CDS | 75531605 | 75531787 | 75531605 | ID=cds.MS.gene062441.t1;Parent=MS.gene062441.t1 |
| chr7.3 | exon | 75531244 | 75531495 | 75531244 | ID=MS.gene062441.t1.exon2;Parent=MS.gene062441.t1 |
| chr7.3 | CDS | 75531244 | 75531495 | 75531244 | ID=cds.MS.gene062441.t1;Parent=MS.gene062441.t1 |
| Gene Sequence |
| Protein sequence |
