Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062478.t1 | AES78040.1 | 97.8 | 183 | 4 | 0 | 1 | 183 | 1 | 183 | 1.10E-89 | 339.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062478.t1 | Q5QMM3 | 48.6 | 179 | 65 | 4 | 1 | 152 | 1 | 179 | 5.1e-32 | 139.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062478.t1 | G7KVS3 | 97.8 | 183 | 4 | 0 | 1 | 183 | 1 | 183 | 7.8e-90 | 339.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene062478.t1 | TF | HB-WOX |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062478.t1 | MTR_7g023810 | 97.814 | 183 | 4 | 0 | 1 | 183 | 1 | 183 | 1.46e-132 | 369 |
| MS.gene062478.t1 | MTR_1g115315 | 61.828 | 186 | 49 | 5 | 1 | 165 | 5 | 189 | 6.63e-68 | 208 |
| MS.gene062478.t1 | MTR_1g115315 | 61.828 | 186 | 49 | 5 | 1 | 165 | 5 | 189 | 9.74e-68 | 207 |
| MS.gene062478.t1 | MTR_3g115620 | 45.550 | 191 | 78 | 4 | 9 | 176 | 7 | 194 | 9.69e-47 | 152 |
| MS.gene062478.t1 | MTR_4g084550 | 33.071 | 127 | 73 | 3 | 36 | 150 | 25 | 151 | 2.60e-14 | 67.8 |
| MS.gene062478.t1 | MTR_8g107210 | 48.387 | 62 | 32 | 0 | 68 | 129 | 96 | 157 | 1.86e-13 | 67.8 |
| MS.gene062478.t1 | MTR_2g015000 | 42.424 | 66 | 38 | 0 | 62 | 127 | 49 | 114 | 8.33e-13 | 65.9 |
| MS.gene062478.t1 | MTR_4g088080 | 45.000 | 60 | 33 | 0 | 66 | 125 | 38 | 97 | 2.14e-12 | 64.3 |
| MS.gene062478.t1 | MTR_4g088070 | 41.270 | 63 | 37 | 0 | 66 | 128 | 27 | 89 | 2.66e-11 | 61.6 |
| MS.gene062478.t1 | MTR_4g063735 | 45.161 | 62 | 34 | 0 | 68 | 129 | 25 | 86 | 2.66e-11 | 60.8 |
| MS.gene062478.t1 | MTR_1g019130 | 31.304 | 115 | 72 | 2 | 53 | 164 | 79 | 189 | 2.70e-11 | 60.5 |
| MS.gene062478.t1 | MTR_7g025010 | 40.625 | 64 | 38 | 0 | 66 | 129 | 45 | 108 | 4.62e-11 | 61.2 |
| MS.gene062478.t1 | MTR_5g081990 | 31.193 | 109 | 60 | 2 | 68 | 175 | 33 | 127 | 6.12e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062478.t1 | AT4G35550 | 57.895 | 152 | 44 | 2 | 17 | 148 | 33 | 184 | 3.30e-48 | 158 |
| MS.gene062478.t1 | AT1G20700 | 48.000 | 150 | 55 | 4 | 15 | 141 | 24 | 173 | 4.31e-37 | 127 |
| MS.gene062478.t1 | AT1G20700 | 48.000 | 150 | 55 | 4 | 15 | 141 | 24 | 173 | 6.12e-37 | 127 |
| MS.gene062478.t1 | AT1G20710 | 47.244 | 127 | 47 | 2 | 21 | 128 | 14 | 139 | 1.03e-26 | 100 |
| MS.gene062478.t1 | AT1G20700 | 55.556 | 81 | 31 | 1 | 66 | 141 | 31 | 111 | 4.64e-24 | 92.4 |
| MS.gene062478.t1 | AT2G33880 | 42.424 | 66 | 38 | 0 | 62 | 127 | 49 | 114 | 1.32e-12 | 65.5 |
| MS.gene062478.t1 | AT2G33880 | 42.424 | 66 | 38 | 0 | 62 | 127 | 49 | 114 | 1.34e-12 | 65.5 |
| MS.gene062478.t1 | AT3G18010 | 45.161 | 62 | 34 | 0 | 68 | 129 | 76 | 137 | 1.81e-12 | 64.7 |
| MS.gene062478.t1 | AT5G45980 | 43.548 | 62 | 35 | 0 | 66 | 127 | 53 | 114 | 2.83e-12 | 64.3 |
| MS.gene062478.t1 | AT2G28610 | 43.333 | 60 | 34 | 0 | 68 | 127 | 8 | 67 | 5.92e-11 | 59.7 |
| MS.gene062478.t1 | AT2G01500 | 43.636 | 55 | 31 | 0 | 68 | 122 | 61 | 115 | 7.84e-11 | 59.7 |
Find 36 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGAGACCAGTGTGTATAAT+TGG | 0.216618 | 7.3:+75930985 | MS.gene062478:CDS |
| TTTGCAGTTGCACAGGTGTT+GGG | 0.322381 | 7.3:-75930869 | None:intergenic |
| TGTATAATTGGTTTCAGAAT+AGG | 0.361284 | 7.3:+75930997 | MS.gene062478:CDS |
| GAGATCTTGTTGAGAAGATA+AGG | 0.380614 | 7.3:-75930622 | None:intergenic |
| CTTTGCAGTTGCACAGGTGT+TGG | 0.384218 | 7.3:-75930870 | None:intergenic |
| TCCAAGCAAGGAAAAGATAA+AGG | 0.403964 | 7.3:+75930926 | MS.gene062478:CDS |
| ACCTGTGCAACTGCAAAGTC+TGG | 0.404137 | 7.3:+75930875 | MS.gene062478:CDS |
| TCAACCTCAGTCTCTACTTC+CGG | 0.438371 | 7.3:-75931065 | None:intergenic |
| CTCTGAAATCTGCCCGTGCT+TGG | 0.446639 | 7.3:-75930967 | None:intergenic |
| AAATAATGTTAATGATGAAC+CGG | 0.459463 | 7.3:+75931046 | MS.gene062478:CDS |
| GACTGAGATGCAACGATCTC+CGG | 0.461176 | 7.3:-75931110 | None:intergenic |
| CGAATATTTGAAGCTGAGAC+GGG | 0.462716 | 7.3:+75930900 | MS.gene062478:CDS |
| ATGAACAAGTCGAAACTCTT+CGG | 0.463263 | 7.3:+75930544 | MS.gene062478:CDS |
| TCGTAAACTGTCATCTGATT+CGG | 0.469903 | 7.3:-75931174 | None:intergenic |
| CACTTGCATCATTCCAACAT+TGG | 0.480071 | 7.3:-75930508 | None:intergenic |
| AATTCAAGTCCGCTAAGTAC+TGG | 0.486943 | 7.3:-75931151 | None:intergenic |
| TCCAGACTTTGCAGTTGCAC+AGG | 0.490183 | 7.3:-75930876 | None:intergenic |
| CAATGATAAGAAAACTGAAC+CGG | 0.495461 | 7.3:+75931091 | MS.gene062478:CDS |
| AAGAGTTTCGACTTGTTCAT+CGG | 0.503160 | 7.3:-75930541 | None:intergenic |
| TTGCAGTTGCACAGGTGTTG+GGG | 0.508107 | 7.3:-75930868 | None:intergenic |
| GGTTTCAGAATAGGCGTGCT+AGG | 0.518890 | 7.3:+75931006 | MS.gene062478:CDS |
| GCGAATATTTGAAGCTGAGA+CGG | 0.525730 | 7.3:+75930899 | MS.gene062478:CDS |
| TTTAATCAGAACGCCAATGT+TGG | 0.533338 | 7.3:+75930495 | MS.gene062478:CDS |
| GAATAGGCGTGCTAGGTCAA+AGG | 0.534265 | 7.3:+75931013 | MS.gene062478:CDS |
| AATAGGCGTGCTAGGTCAAA+GGG | 0.534387 | 7.3:+75931014 | MS.gene062478:CDS |
| ACCGCTGATTTGACCAAGCA+CGG | 0.535092 | 7.3:+75930954 | MS.gene062478:CDS |
| CAACAAGATCTCACTCTCAC+TGG | 0.535564 | 7.3:+75930633 | MS.gene062478:CDS |
| TTTGTGCAGCCAGTACTTAG+CGG | 0.536719 | 7.3:+75931142 | MS.gene062478:CDS |
| TTTCAGGGTCTACTAGGCTG+AGG | 0.572469 | 7.3:+75930835 | MS.gene062478:intron |
| CTACTAGGCTGAGGCAACGT+TGG | 0.578621 | 7.3:+75930844 | MS.gene062478:CDS |
| CCCGTGCTTGGTCAAATCAG+CGG | 0.631243 | 7.3:-75930955 | None:intergenic |
| TGAGACGGGGACTCCAAGCA+AGG | 0.638243 | 7.3:+75930914 | MS.gene062478:CDS |
| CCGCTGATTTGACCAAGCAC+GGG | 0.640635 | 7.3:+75930955 | MS.gene062478:CDS |
| CTGAAACCAATTATACACAC+TGG | 0.642488 | 7.3:-75930991 | None:intergenic |
| GAATATTTGAAGCTGAGACG+GGG | 0.658394 | 7.3:+75930901 | MS.gene062478:CDS |
| TGAACCGGAAGTAGAGACTG+AGG | 0.698771 | 7.3:+75931061 | MS.gene062478:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAATGTTAATGATGAAC+CGG | + | chr7.3:75931046-75931065 | MS.gene062478:CDS | 20.0% |
| !!! | AACATTTAATGATGATTTTC+AGG | + | chr7.3:75930819-75930838 | MS.gene062478:intron | 20.0% |
| !!! | ACATTTAATGATGATTTTCA+GGG | + | chr7.3:75930820-75930839 | MS.gene062478:intron | 20.0% |
| !! | TGTATAATTGGTTTCAGAAT+AGG | + | chr7.3:75930997-75931016 | MS.gene062478:CDS | 25.0% |
| CAATGATAAGAAAACTGAAC+CGG | + | chr7.3:75931091-75931110 | MS.gene062478:CDS | 30.0% | |
| ATGAACAAGTCGAAACTCTT+CGG | + | chr7.3:75930544-75930563 | MS.gene062478:CDS | 35.0% | |
| CTGAAACCAATTATACACAC+TGG | - | chr7.3:75930994-75931013 | None:intergenic | 35.0% | |
| GAGATCTTGTTGAGAAGATA+AGG | - | chr7.3:75930625-75930644 | None:intergenic | 35.0% | |
| GTTCAGTTACTCATTCTCAA+TGG | + | chr7.3:75930677-75930696 | MS.gene062478:intron | 35.0% | |
| TAATGAACCGGTCTTTAGTA+CGG | + | chr7.3:75930734-75930753 | MS.gene062478:intron | 35.0% | |
| TCAATGGTATCACAGTTAAC+CGG | + | chr7.3:75930693-75930712 | MS.gene062478:intron | 35.0% | |
| TCCAAGCAAGGAAAAGATAA+AGG | + | chr7.3:75930926-75930945 | MS.gene062478:CDS | 35.0% | |
| TCGTAAACTGTCATCTGATT+CGG | - | chr7.3:75931177-75931196 | None:intergenic | 35.0% | |
| TTTAATCAGAACGCCAATGT+TGG | + | chr7.3:75930495-75930514 | MS.gene062478:CDS | 35.0% | |
| ! | AAGAGTTTCGACTTGTTCAT+CGG | - | chr7.3:75930544-75930563 | None:intergenic | 35.0% |
| ! | ACCGGTTCATTATATTGAAC+CGG | - | chr7.3:75930726-75930745 | None:intergenic | 35.0% |
| ! | CGGTTCAGTTTTCTTATCAT+TGG | - | chr7.3:75931093-75931112 | None:intergenic | 35.0% |
| ! | TCCTTTATCTTTTCCTTGCT+TGG | - | chr7.3:75930930-75930949 | None:intergenic | 35.0% |
| AACCAAACCGTACTAAAGAC+CGG | - | chr7.3:75930744-75930763 | None:intergenic | 40.0% | |
| AATTCAAGTCCGCTAAGTAC+TGG | - | chr7.3:75931154-75931173 | None:intergenic | 40.0% | |
| CAATGGTATCACAGTTAACC+GGG | + | chr7.3:75930694-75930713 | MS.gene062478:intron | 40.0% | |
| CACTTGCATCATTCCAACAT+TGG | - | chr7.3:75930511-75930530 | None:intergenic | 40.0% | |
| CAGAGACCAGTGTGTATAAT+TGG | + | chr7.3:75930985-75931004 | MS.gene062478:CDS | 40.0% | |
| CCCGGTTCAATATAATGAAC+CGG | + | chr7.3:75930722-75930741 | MS.gene062478:intron | 40.0% | |
| CCGGTTCATTATATTGAACC+GGG | - | chr7.3:75930725-75930744 | None:intergenic | 40.0% | |
| ! | CGAATATTTGAAGCTGAGAC+GGG | + | chr7.3:75930900-75930919 | MS.gene062478:CDS | 40.0% |
| ! | GAATATTTGAAGCTGAGACG+GGG | + | chr7.3:75930901-75930920 | MS.gene062478:CDS | 40.0% |
| ! | GCGAATATTTGAAGCTGAGA+CGG | + | chr7.3:75930899-75930918 | MS.gene062478:CDS | 40.0% |
| !! | GATGATTTTCAGGGTCTACT+AGG | + | chr7.3:75930829-75930848 | MS.gene062478:intron | 40.0% |
| AACCGGTCTTTAGTACGGTT+TGG | + | chr7.3:75930739-75930758 | MS.gene062478:intron | 45.0% | |
| AATAGGCGTGCTAGGTCAAA+GGG | + | chr7.3:75931014-75931033 | MS.gene062478:CDS | 45.0% | |
| CAACAAGATCTCACTCTCAC+TGG | + | chr7.3:75930633-75930652 | MS.gene062478:CDS | 45.0% | |
| TCAACCTCAGTCTCTACTTC+CGG | - | chr7.3:75931068-75931087 | None:intergenic | 45.0% | |
| TTTGCAGTTGCACAGGTGTT+GGG | - | chr7.3:75930872-75930891 | None:intergenic | 45.0% | |
| TTTGTGCAGCCAGTACTTAG+CGG | + | chr7.3:75931142-75931161 | MS.gene062478:CDS | 45.0% | |
| ACCTGTGCAACTGCAAAGTC+TGG | + | chr7.3:75930875-75930894 | MS.gene062478:CDS | 50.0% | |
| CTTTGCAGTTGCACAGGTGT+TGG | - | chr7.3:75930873-75930892 | None:intergenic | 50.0% | |
| GAATAGGCGTGCTAGGTCAA+AGG | + | chr7.3:75931013-75931032 | MS.gene062478:CDS | 50.0% | |
| GACTGAGATGCAACGATCTC+CGG | - | chr7.3:75931113-75931132 | None:intergenic | 50.0% | |
| GGTTTCAGAATAGGCGTGCT+AGG | + | chr7.3:75931006-75931025 | MS.gene062478:CDS | 50.0% | |
| TCCAGACTTTGCAGTTGCAC+AGG | - | chr7.3:75930879-75930898 | None:intergenic | 50.0% | |
| TGAACCGGAAGTAGAGACTG+AGG | + | chr7.3:75931061-75931080 | MS.gene062478:CDS | 50.0% | |
| TGGTATCACAGTTAACCGGG+TGG | + | chr7.3:75930697-75930716 | MS.gene062478:intron | 50.0% | |
| TTTCAGGGTCTACTAGGCTG+AGG | + | chr7.3:75930835-75930854 | MS.gene062478:intron | 50.0% | |
| ! | TTGCAGTTGCACAGGTGTTG+GGG | - | chr7.3:75930871-75930890 | None:intergenic | 50.0% |
| !! | ACCGCTGATTTGACCAAGCA+CGG | + | chr7.3:75930954-75930973 | MS.gene062478:CDS | 50.0% |
| ATATTGAACCGGGCTCCACC+CGG | - | chr7.3:75930715-75930734 | None:intergenic | 55.0% | |
| CCCGTGCTTGGTCAAATCAG+CGG | - | chr7.3:75930958-75930977 | None:intergenic | 55.0% | |
| CTACTAGGCTGAGGCAACGT+TGG | + | chr7.3:75930844-75930863 | MS.gene062478:CDS | 55.0% | |
| !! | CCGCTGATTTGACCAAGCAC+GGG | + | chr7.3:75930955-75930974 | MS.gene062478:CDS | 55.0% |
| !! | CTCTGAAATCTGCCCGTGCT+TGG | - | chr7.3:75930970-75930989 | None:intergenic | 55.0% |
| ACAGTTAACCGGGTGGAGCC+CGG | + | chr7.3:75930704-75930723 | MS.gene062478:intron | 60.0% | |
| TGAGACGGGGACTCCAAGCA+AGG | + | chr7.3:75930914-75930933 | MS.gene062478:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 75930474 | 75931211 | 75930474 | ID=MS.gene062478 |
| chr7.3 | mRNA | 75930474 | 75931211 | 75930474 | ID=MS.gene062478.t1;Parent=MS.gene062478 |
| chr7.3 | exon | 75930474 | 75930654 | 75930474 | ID=MS.gene062478.t1.exon1;Parent=MS.gene062478.t1 |
| chr7.3 | CDS | 75930474 | 75930654 | 75930474 | ID=cds.MS.gene062478.t1;Parent=MS.gene062478.t1 |
| chr7.3 | exon | 75930841 | 75931211 | 75930841 | ID=MS.gene062478.t1.exon2;Parent=MS.gene062478.t1 |
| chr7.3 | CDS | 75930841 | 75931211 | 75930841 | ID=cds.MS.gene062478.t1;Parent=MS.gene062478.t1 |
| Gene Sequence |
| Protein sequence |
