AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062918

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062918.t1 MTR_4g071590 96.622 296 9 1 1 296 1 295 0.0 594
MS.gene062918.t1 MTR_4g471590 96.622 296 9 1 1 296 1 295 0.0 594
MS.gene062918.t1 MTR_3g117380 53.292 319 107 8 1 296 15 314 3.27e-97 289
MS.gene062918.t1 MTR_8g076340 39.159 309 138 10 1 281 1 287 1.23e-51 171
MS.gene062918.t1 MTR_5g021340 40.282 355 137 11 1 296 51 389 4.63e-50 170
MS.gene062918.t1 MTR_3g109760 38.406 276 120 9 36 281 21 276 4.96e-45 155
MS.gene062918.t1 MTR_8g013950 39.024 287 107 10 39 281 33 295 3.29e-43 151
MS.gene062918.t1 MTR_1g006650 34.921 315 141 12 15 281 5 303 2.23e-42 149
MS.gene062918.t1 MTR_1g094930 75.714 70 17 0 207 276 142 211 5.39e-35 127
MS.gene062918.t1 MTR_1g094930 75.714 70 17 0 207 276 237 306 1.67e-34 128
MS.gene062918.t1 MTR_6g032830 81.538 65 12 0 212 276 192 256 2.70e-34 125
MS.gene062918.t1 MTR_7g085410 80.000 65 13 0 212 276 188 252 2.91e-33 123
MS.gene062918.t1 MTR_1g116010 67.532 77 25 0 212 288 164 240 4.80e-33 122
MS.gene062918.t1 MTR_1g046680 76.471 68 16 0 212 279 172 239 1.17e-32 120
MS.gene062918.t1 MTR_2g094600 62.791 86 30 1 191 276 197 280 3.51e-32 121
MS.gene062918.t1 MTR_4g058910 73.134 67 18 0 212 278 314 380 1.59e-31 121
MS.gene062918.t1 MTR_5g007600 68.571 70 22 0 212 281 259 328 1.22e-29 115
MS.gene062918.t1 MTR_5g007600 59.036 83 28 1 199 275 138 220 2.74e-26 105
MS.gene062918.t1 MTR_7g446110 57.143 63 27 0 211 273 186 248 2.12e-20 88.6
MS.gene062918.t1 MTR_7g445950 30.128 156 105 1 118 273 30 181 2.55e-20 86.7
MS.gene062918.t1 MTR_7g445980 51.316 76 36 1 199 273 20 95 3.06e-19 81.3
MS.gene062918.t1 MTR_7g446070 31.868 182 107 5 105 273 16 193 7.10e-18 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062918.t1 AT4G36240 47.082 257 90 10 40 282 10 234 1.42e-56 182
MS.gene062918.t1 AT5G66320 35.838 346 139 10 8 291 5 329 2.87e-51 172
MS.gene062918.t1 AT5G66320 35.838 346 139 10 8 291 5 329 2.87e-51 172
MS.gene062918.t1 AT4G34680 41.034 290 114 11 6 283 7 251 1.97e-49 165
MS.gene062918.t1 AT4G34680 41.034 290 114 11 6 283 7 251 1.97e-49 165
MS.gene062918.t1 AT5G25830 38.047 297 108 12 40 281 13 288 1.36e-44 154
MS.gene062918.t1 AT3G60530 47.778 180 73 5 112 283 63 229 2.29e-43 149
MS.gene062918.t1 AT4G32890 36.331 278 115 11 39 281 16 266 4.14e-43 150
MS.gene062918.t1 AT2G45050 44.845 194 73 4 112 281 65 248 5.03e-43 148
MS.gene062918.t1 AT3G51080 69.231 91 22 2 212 296 221 311 2.37e-38 137
MS.gene062918.t1 AT3G24050 64.706 85 29 1 197 280 178 262 9.79e-34 124
MS.gene062918.t1 AT3G54810 75.000 68 17 0 212 279 229 296 3.72e-33 124
MS.gene062918.t1 AT3G54810 75.000 68 17 0 212 279 229 296 3.72e-33 124
MS.gene062918.t1 AT1G08010 72.464 69 19 0 212 280 220 288 3.61e-32 121
MS.gene062918.t1 AT1G08010 72.464 69 19 0 212 280 220 288 3.61e-32 121
MS.gene062918.t1 AT1G08010 72.464 69 19 0 212 280 220 288 3.61e-32 121
MS.gene062918.t1 AT1G08000 71.014 69 20 0 212 280 218 286 2.82e-30 116
MS.gene062918.t1 AT1G08000 71.014 69 20 0 212 280 218 286 2.82e-30 116
MS.gene062918.t1 AT3G45170 60.274 73 29 0 214 286 117 189 9.59e-27 104
MS.gene062918.t1 AT2G28340 63.077 65 24 0 214 278 193 257 1.70e-24 100

Find 89 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTGTCTTGGTTGTTCT+AGG 0.195513 4.2:+35975616 None:intergenic
AGCCCCACCCGTTTGGGCTT+CGG 0.226880 4.2:+35975528 None:intergenic
GCCCCACCCGTTTGGGCTTC+GGG 0.232140 4.2:+35975529 None:intergenic
GGCCGATGATGTTGCGGATT+TGG 0.241470 4.2:-35975773 MS.gene062918:CDS
GAACAGCCACCGTAAGGTTT+TGG 0.270098 4.2:-35975332 MS.gene062918:CDS
GAGGTGGATGAACCGGTTTC+CGG 0.310815 4.2:-35975292 MS.gene062918:CDS
GGTTCGGCCCGGGCCTGAAC+TGG 0.312951 4.2:+35975697 None:intergenic
TGCCGGTGGTTTAGCAGTTC+CGG 0.331211 4.2:-35976055 MS.gene062918:intron
GAAACTGAGCCCCACCCGTT+TGG 0.331959 4.2:+35975521 None:intergenic
TCTTTGCTGAAAAGAGTATA+GGG 0.337151 4.2:+35975566 None:intergenic
TCTCTTCACAAATTGTTTGT+TGG 0.346081 4.2:+35976334 None:intergenic
AAACTGAGCCCCACCCGTTT+GGG 0.350163 4.2:+35975522 None:intergenic
CTTCTTCTTCTTCCTCCTCC+TGG 0.352825 4.2:+35976193 None:intergenic
CTCTCTATTGTATCCAGTTC+AGG 0.364580 4.2:-35975710 MS.gene062918:CDS
AACCGGTTTCCGGTCTGAAC+CGG 0.370991 4.2:-35975282 MS.gene062918:CDS
GCATTGGTTGGGCCCGGTCT+AGG 0.372639 4.2:+35975670 None:intergenic
ACAGTTGGGCCTTCTTGTCT+TGG 0.379924 4.2:+35975606 None:intergenic
GTTGGGCCCGGTCTAGGTTC+CGG 0.399228 4.2:+35975676 None:intergenic
AGTTCAGGCCCGGGCCGAAC+CGG 0.407384 4.2:-35975695 MS.gene062918:CDS
GTCTAGGTTCCGGTTCGGCC+CGG 0.414385 4.2:+35975686 None:intergenic
GGCCGAACCGGAACCTAGAC+CGG 0.414860 4.2:-35975683 MS.gene062918:CDS
TTCTTTGCTGAAAAGAGTAT+AGG 0.416988 4.2:+35975565 None:intergenic
AACGCAAACAACGTTGTTGT+CGG 0.417578 4.2:-35976300 MS.gene062918:CDS
TTTGGAAATGAGGCGGAGGA+AGG 0.432787 4.2:-35975314 MS.gene062918:CDS
TATGTAACGCTTGTGGTGTT+CGG 0.433641 4.2:-35975426 MS.gene062918:CDS
GAATTTCACCGGAAAACGTC+GGG 0.435549 4.2:+35975359 None:intergenic
GAATTTCAACATGGTTCTGT+TGG 0.444517 4.2:-35976234 MS.gene062918:CDS
TATTGTATCCAGTTCAGGCC+CGG 0.446736 4.2:-35975705 MS.gene062918:CDS
GGAATGGGTGTCTCACTTTG+TGG 0.449579 4.2:-35975752 MS.gene062918:CDS
AATGAGGCGGAGGAAGGAGG+TGG 0.451531 4.2:-35975308 MS.gene062918:CDS
ATCCGGTTCAGACCGGAAAC+CGG 0.452815 4.2:+35975280 None:intergenic
GAGGAAGGAGGTGGATGAAC+CGG 0.452883 4.2:-35975299 MS.gene062918:CDS
TCTAGGTTCCGGTTCGGCCC+GGG 0.468440 4.2:+35975687 None:intergenic
ATAGATTCTGTCCTCAAGCT+TGG 0.468461 4.2:+35976360 None:intergenic
GCCTCATTTCCAAAACCTTA+CGG 0.469337 4.2:+35975323 None:intergenic
TTCTGCGGATTATAGAGAGC+TGG 0.472534 4.2:+35975641 None:intergenic
CGGTCTGAACCGGATTCAAA+TGG 0.475660 4.2:-35975272 MS.gene062918:CDS
ATGATGTTGCGGATTTGGAA+TGG 0.479723 4.2:-35975768 MS.gene062918:CDS
AACTCGTGACCATTTGAATC+CGG 0.481371 4.2:+35975263 None:intergenic
CAGTGGAGAGCTGGTCCGCT+TGG 0.483307 4.2:-35975460 MS.gene062918:CDS
TGTGGTGTTCGGTTTAAGTC+CGG 0.492368 4.2:-35975415 MS.gene062918:CDS
AGACTCGCCGTCCCCGGTAC+CGG 0.492865 4.2:+35976089 None:intergenic
GGATACAATAGAGAGAGTTC+TGG 0.493646 4.2:+35975718 None:intergenic
GCCCGAAGCCCAAACGGGTG+GGG 0.493923 4.2:-35975530 MS.gene062918:CDS
AAAATCCACACGTTACAGTT+GGG 0.498357 4.2:+35975592 None:intergenic
TGATGTTGCGGATTTGGAAT+GGG 0.501793 4.2:-35975767 MS.gene062918:CDS
GGAAATGAGGCGGAGGAAGG+AGG 0.505698 4.2:-35975311 MS.gene062918:CDS
GCCCGGTCTAGGTTCCGGTT+CGG 0.510484 4.2:+35975681 None:intergenic
AGCTGGGAATAAGCATTGGT+TGG 0.510903 4.2:+35975658 None:intergenic
TTCTCAGGCCGATGATGTTG+CGG 0.519029 4.2:-35975779 MS.gene062918:intron
AAAAGAAATTGATGTTTACC+AGG 0.530934 4.2:-35976211 MS.gene062918:CDS
GCTGGGAATAAGCATTGGTT+GGG 0.531676 4.2:+35975659 None:intergenic
GAAAACGTCGGGCTGCATGC+AGG 0.532133 4.2:+35975370 None:intergenic
AAAACGCCACAGTGGAGAGC+TGG 0.532352 4.2:-35975469 MS.gene062918:CDS
AATTGATGTTTACCAGGAGG+AGG 0.533216 4.2:-35976205 MS.gene062918:CDS
TTCCAAATCCGCAACATCAT+CGG 0.540001 4.2:+35975771 None:intergenic
AGCCCGAAGCCCAAACGGGT+GGG 0.541657 4.2:-35975531 MS.gene062918:CDS
GAATAAGCATTGGTTGGGCC+CGG 0.542060 4.2:+35975664 None:intergenic
ATTGTATCCAGTTCAGGCCC+GGG 0.543140 4.2:-35975704 MS.gene062918:CDS
CTGCATGCAGGTCTATACTC+AGG 0.544535 4.2:+35975382 None:intergenic
GGAGTCAGACTCGCCGTCCC+CGG 0.547526 4.2:+35976083 None:intergenic
AGAGAGCTGGGAATAAGCAT+TGG 0.549150 4.2:+35975654 None:intergenic
GCCGAACCGGAACCTAGACC+GGG 0.552879 4.2:-35975682 MS.gene062918:CDS
TCTGCGGATTATAGAGAGCT+GGG 0.553907 4.2:+35975642 None:intergenic
CATTTGAATCCGGTTCAGAC+CGG 0.555998 4.2:+35975273 None:intergenic
GCTGTTCGAATGAATTTCAC+CGG 0.556294 4.2:+35975348 None:intergenic
CCCGGTACCGGAAAATGTTG+TGG 0.557351 4.2:+35976101 None:intergenic
AAGCCCGAAGCCCAAACGGG+TGG 0.557944 4.2:-35975532 MS.gene062918:CDS
TCATTCGAACAGCCACCGTA+AGG 0.576237 4.2:-35975338 MS.gene062918:CDS
ATACTCAGGGAAAAGCCGAC+CGG 0.581357 4.2:+35975396 None:intergenic
CTAGAACAACCAAGACAAGA+AGG 0.581886 4.2:-35975615 MS.gene062918:CDS
ACCATGTTGAAATTCACCGT+TGG 0.582797 4.2:+35976242 None:intergenic
CACCGGAACTGCTAAACCAC+CGG 0.593758 4.2:+35976053 None:intergenic
TGGTTGTTCTAGGCTTTCTG+CGG 0.603627 4.2:+35975626 None:intergenic
TGAATTTCACCGGAAAACGT+CGG 0.605768 4.2:+35975358 None:intergenic
AAACATGGAAGTTGTTGTAG+CGG 0.614309 4.2:-35976394 None:intergenic
TGCATGCAGGTCTATACTCA+GGG 0.615040 4.2:+35975383 None:intergenic
TCGTTCATGTTCAGAACCTG+AGG 0.628083 4.2:+35976149 None:intergenic
TTCATGTTCAGAACCTGAGG+TGG 0.629594 4.2:+35976152 None:intergenic
GTGTTCGGTTTAAGTCCGGT+CGG 0.631814 4.2:-35975411 MS.gene062918:CDS
TCCAACGGTGAATTTCAACA+TGG 0.652718 4.2:-35976243 MS.gene062918:CDS
GAAGGCCCAACTGTAACGTG+TGG 0.654146 4.2:-35975597 MS.gene062918:CDS
AAAACACTATGTAACGCTTG+TGG 0.656575 4.2:-35975433 MS.gene062918:CDS
AGAAATTGATGTTTACCAGG+AGG 0.667546 4.2:-35976208 MS.gene062918:CDS
AAGCGTTGAAACCAAGCTTG+AGG 0.681951 4.2:-35976371 MS.gene062918:CDS
TCATTTCCAAAACCTTACGG+TGG 0.687073 4.2:+35975326 None:intergenic
AGCGGACCAGCTCTCCACTG+TGG 0.697012 4.2:+35975463 None:intergenic
ATGTTCAGAAAACGCCACAG+TGG 0.715931 4.2:-35975477 MS.gene062918:CDS
GTGGGGCTCAGTTTCAACGA+CGG 0.724625 4.2:-35975513 MS.gene062918:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAAATAATTTATTGCA+TGG + chr4.2:35975633-35975652 None:intergenic 15.0%
!!! ATATAACTAATAACTCGTTT+TGG - chr4.2:35975840-35975859 MS.gene062918:intron 20.0%
! AAAAGAAATTGATGTTTACC+AGG - chr4.2:35975440-35975459 MS.gene062918:CDS 25.0%
!!! TTTTGGTTTTCTTAATTCTC+AGG - chr4.2:35975857-35975876 MS.gene062918:intron 25.0%
AATTCATTACCTTTGTAGCA+TGG - chr4.2:35975672-35975691 MS.gene062918:CDS 30.0%
TCTCTTCACAAATTGTTTGT+TGG + chr4.2:35975320-35975339 None:intergenic 30.0%
! TCAAATTCCACAACATTTTC+CGG - chr4.2:35975543-35975562 MS.gene062918:CDS 30.0%
! TCTTTGCTGAAAAGAGTATA+GGG + chr4.2:35976088-35976107 None:intergenic 30.0%
! TTCTTTGCTGAAAAGAGTAT+AGG + chr4.2:35976089-35976108 None:intergenic 30.0%
AAAAATCCACACGTTACAGT+TGG + chr4.2:35976063-35976082 None:intergenic 35.0%
AAAACACTATGTAACGCTTG+TGG - chr4.2:35976218-35976237 MS.gene062918:CDS 35.0%
AAAATCCACACGTTACAGTT+GGG + chr4.2:35976062-35976081 None:intergenic 35.0%
AGAAATTGATGTTTACCAGG+AGG - chr4.2:35975443-35975462 MS.gene062918:CDS 35.0%
TATCACTAGTATCATGTGTC+CGG - chr4.2:35975784-35975803 MS.gene062918:intron 35.0%
! CAAGCGTTACATAGTGTTTT+CGG + chr4.2:35976218-35976237 None:intergenic 35.0%
! GAATTTCAACATGGTTCTGT+TGG - chr4.2:35975417-35975436 MS.gene062918:CDS 35.0%
!! GACTTGCTTGATTTTTCCAA+CGG - chr4.2:35975393-35975412 MS.gene062918:CDS 35.0%
AACGCAAACAACGTTGTTGT+CGG - chr4.2:35975351-35975370 MS.gene062918:CDS 40.0%
AACTCGTGACCATTTGAATC+CGG + chr4.2:35976391-35976410 None:intergenic 40.0%
AATTGATGTTTACCAGGAGG+AGG - chr4.2:35975446-35975465 MS.gene062918:CDS 40.0%
ACCATGTTGAAATTCACCGT+TGG + chr4.2:35975412-35975431 None:intergenic 40.0%
ATAGATTCTGTCCTCAAGCT+TGG + chr4.2:35975294-35975313 None:intergenic 40.0%
ATGATGTTGCGGATTTGGAA+TGG - chr4.2:35975883-35975902 MS.gene062918:intron 40.0%
CAGAAATGTCCATGCTACAA+AGG + chr4.2:35975684-35975703 None:intergenic 40.0%
CTAGAACAACCAAGACAAGA+AGG - chr4.2:35976036-35976055 MS.gene062918:intron 40.0%
CTCTCTATTGTATCCAGTTC+AGG - chr4.2:35975941-35975960 MS.gene062918:intron 40.0%
CTTCTTGTCTTGGTTGTTCT+AGG + chr4.2:35976038-35976057 None:intergenic 40.0%
GCCTCATTTCCAAAACCTTA+CGG + chr4.2:35976331-35976350 None:intergenic 40.0%
GCTGTTCGAATGAATTTCAC+CGG + chr4.2:35976306-35976325 None:intergenic 40.0%
GGATACAATAGAGAGAGTTC+TGG + chr4.2:35975936-35975955 None:intergenic 40.0%
TATGTAACGCTTGTGGTGTT+CGG - chr4.2:35976225-35976244 MS.gene062918:CDS 40.0%
TCATTTCCAAAACCTTACGG+TGG + chr4.2:35976328-35976347 None:intergenic 40.0%
TCCAACGGTGAATTTCAACA+TGG - chr4.2:35975408-35975427 MS.gene062918:CDS 40.0%
TGAATTTCACCGGAAAACGT+CGG + chr4.2:35976296-35976315 None:intergenic 40.0%
TTCCAAATCCGCAACATCAT+CGG + chr4.2:35975883-35975902 None:intergenic 40.0%
TTGCATGGTTAAAGACTCAC+CGG + chr4.2:35975618-35975637 None:intergenic 40.0%
! TCTTTTCAGCAAAGAAGCAG+AGG - chr4.2:35976093-35976112 MS.gene062918:CDS 40.0%
! TGATGTTGCGGATTTGGAAT+GGG - chr4.2:35975884-35975903 MS.gene062918:intron 40.0%
!!! ACCGTAAGGTTTTGGAAATG+AGG - chr4.2:35976327-35976346 MS.gene062918:CDS 40.0%
!!! GTAAGGTTTTGGAAATGAGG+CGG - chr4.2:35976330-35976349 MS.gene062918:CDS 40.0%
ACTAGTATCATGTGTCCGGT+CGG - chr4.2:35975788-35975807 MS.gene062918:intron 45.0%
AGAGAGCTGGGAATAAGCAT+TGG + chr4.2:35976000-35976019 None:intergenic 45.0%
ATGTTCAGAAAACGCCACAG+TGG - chr4.2:35976174-35976193 MS.gene062918:CDS 45.0%
CATTTGAATCCGGTTCAGAC+CGG + chr4.2:35976381-35976400 None:intergenic 45.0%
GAATTTCACCGGAAAACGTC+GGG + chr4.2:35976295-35976314 None:intergenic 45.0%
GTCGTGTTAGTGACATGTGT+GGG + chr4.2:35975726-35975745 None:intergenic 45.0%
TATTGTATCCAGTTCAGGCC+CGG - chr4.2:35975946-35975965 MS.gene062918:intron 45.0%
TCGTTCATGTTCAGAACCTG+AGG + chr4.2:35975505-35975524 None:intergenic 45.0%
TGCATGCAGGTCTATACTCA+GGG + chr4.2:35976271-35976290 None:intergenic 45.0%
TGCTAAACCACCGGCAAAAA+TGG + chr4.2:35975592-35975611 None:intergenic 45.0%
TGTCGTGTTAGTGACATGTG+TGG + chr4.2:35975727-35975746 None:intergenic 45.0%
TGTGGTGTTCGGTTTAAGTC+CGG - chr4.2:35976236-35976255 MS.gene062918:CDS 45.0%
TTCATGTTCAGAACCTGAGG+TGG + chr4.2:35975502-35975521 None:intergenic 45.0%
! ACTGTGGCGTTTTCTGAACA+TGG + chr4.2:35976175-35976194 None:intergenic 45.0%
! CATAGTGTTTTCGGTCCAAG+CGG + chr4.2:35976209-35976228 None:intergenic 45.0%
! TCCACAACATTTTCCGGTAC+CGG - chr4.2:35975549-35975568 MS.gene062918:CDS 45.0%
! TCTGCGGATTATAGAGAGCT+GGG + chr4.2:35976012-35976031 None:intergenic 45.0%
! TGGTTGTTCTAGGCTTTCTG+CGG + chr4.2:35976028-35976047 None:intergenic 45.0%
! TTCTGCGGATTATAGAGAGC+TGG + chr4.2:35976013-35976032 None:intergenic 45.0%
!! AAGCGTTGAAACCAAGCTTG+AGG - chr4.2:35975280-35975299 MS.gene062918:CDS 45.0%
!! AGCTGGGAATAAGCATTGGT+TGG + chr4.2:35975996-35976015 None:intergenic 45.0%
!! GAGTCTGACTCCATTTTTGC+CGG - chr4.2:35975579-35975598 MS.gene062918:CDS 45.0%
!! GCTGGGAATAAGCATTGGTT+GGG + chr4.2:35975995-35976014 None:intergenic 45.0%
AAAAAGCCCGAAGCCCAAAC+GGG - chr4.2:35976116-35976135 MS.gene062918:CDS 50.0%
ACAGTTGGGCCTTCTTGTCT+TGG + chr4.2:35976048-35976067 None:intergenic 50.0%
ATACTCAGGGAAAAGCCGAC+CGG + chr4.2:35976258-35976277 None:intergenic 50.0%
ATTGTATCCAGTTCAGGCCC+GGG - chr4.2:35975947-35975966 MS.gene062918:intron 50.0%
CGGTCTGAACCGGATTCAAA+TGG - chr4.2:35976379-35976398 MS.gene062918:CDS 50.0%
CTGCATGCAGGTCTATACTC+AGG + chr4.2:35976272-35976291 None:intergenic 50.0%
CTTCTTCTTCTTCCTCCTCC+TGG + chr4.2:35975461-35975480 None:intergenic 50.0%
GAAAAAGCCCGAAGCCCAAA+CGG - chr4.2:35976115-35976134 MS.gene062918:CDS 50.0%
GAGAAAAACAGCACCACCTC+AGG - chr4.2:35975486-35975505 MS.gene062918:CDS 50.0%
GGAATGGGTGTCTCACTTTG+TGG - chr4.2:35975899-35975918 MS.gene062918:intron 50.0%
GTGTTCGGTTTAAGTCCGGT+CGG - chr4.2:35976240-35976259 MS.gene062918:CDS 50.0%
TCATTCGAACAGCCACCGTA+AGG - chr4.2:35976313-35976332 MS.gene062918:CDS 50.0%
TTCTCAGGCCGATGATGTTG+CGG - chr4.2:35975872-35975891 MS.gene062918:intron 50.0%
TTTGGAAATGAGGCGGAGGA+AGG - chr4.2:35976337-35976356 MS.gene062918:CDS 50.0%
! CACAACATTTTCCGGTACCG+GGG - chr4.2:35975551-35975570 MS.gene062918:CDS 50.0%
! CCACAACATTTTCCGGTACC+GGG - chr4.2:35975550-35975569 MS.gene062918:CDS 50.0%
! GAACAGCCACCGTAAGGTTT+TGG - chr4.2:35976319-35976338 MS.gene062918:CDS 50.0%
! GCGTTTTCTGAACATGGCAG+TGG + chr4.2:35976169-35976188 None:intergenic 50.0%
!! GAATAAGCATTGGTTGGGCC+CGG + chr4.2:35975990-35976009 None:intergenic 50.0%
!! TCTGACTCCATTTTTGCCGG+TGG - chr4.2:35975582-35975601 MS.gene062918:CDS 50.0%
!!! AGGTTTTGGAAATGAGGCGG+AGG - chr4.2:35976333-35976352 MS.gene062918:CDS 50.0%
AAAACGCCACAGTGGAGAGC+TGG - chr4.2:35976182-35976201 MS.gene062918:CDS 55.0%
AAACTGAGCCCCACCCGTTT+GGG + chr4.2:35976132-35976151 None:intergenic 55.0%
ATCCGGTTCAGACCGGAAAC+CGG + chr4.2:35976374-35976393 None:intergenic 55.0%
CACCGGAACTGCTAAACCAC+CGG + chr4.2:35975601-35975620 None:intergenic 55.0%
GAAGGCCCAACTGTAACGTG+TGG - chr4.2:35976054-35976073 MS.gene062918:intron 55.0%
GACACGACACAAACACCGAC+CGG + chr4.2:35975806-35975825 None:intergenic 55.0%
GAGGAAGGAGGTGGATGAAC+CGG - chr4.2:35976352-35976371 MS.gene062918:CDS 55.0%
GGCCGATGATGTTGCGGATT+TGG - chr4.2:35975878-35975897 MS.gene062918:intron 55.0%
GTGGGGCTCAGTTTCAACGA+CGG - chr4.2:35976138-35976157 MS.gene062918:CDS 55.0%
TGCCGGTGGTTTAGCAGTTC+CGG - chr4.2:35975596-35975615 MS.gene062918:CDS 55.0%
! AACCGGTTTCCGGTCTGAAC+CGG - chr4.2:35976369-35976388 MS.gene062918:CDS 55.0%
! CCCGGTACCGGAAAATGTTG+TGG + chr4.2:35975553-35975572 None:intergenic 55.0%
! GAGGTGGATGAACCGGTTTC+CGG - chr4.2:35976359-35976378 MS.gene062918:CDS 55.0%
!! ACATTTTCCGGTACCGGGGA+CGG - chr4.2:35975555-35975574 MS.gene062918:CDS 55.0%
AATGAGGCGGAGGAAGGAGG+TGG - chr4.2:35976343-35976362 MS.gene062918:CDS 60.0%
GAAAACGTCGGGCTGCATGC+AGG + chr4.2:35976284-35976303 None:intergenic 60.0%
GAAACTGAGCCCCACCCGTT+TGG + chr4.2:35976133-35976152 None:intergenic 60.0%
GGAAATGAGGCGGAGGAAGG+AGG - chr4.2:35976340-35976359 MS.gene062918:CDS 60.0%
! GCATGCAGCCCGACGTTTTC+CGG - chr4.2:35976284-35976303 MS.gene062918:CDS 60.0%
AAGCCCGAAGCCCAAACGGG+TGG - chr4.2:35976119-35976138 MS.gene062918:CDS 65.0%
AGCCCCACCCGTTTGGGCTT+CGG + chr4.2:35976126-35976145 None:intergenic 65.0%
AGCCCGAAGCCCAAACGGGT+GGG - chr4.2:35976120-35976139 MS.gene062918:CDS 65.0%
AGCGGACCAGCTCTCCACTG+TGG + chr4.2:35976191-35976210 None:intergenic 65.0%
CAGTGGAGAGCTGGTCCGCT+TGG - chr4.2:35976191-35976210 MS.gene062918:CDS 65.0%
GCCCGGTCTAGGTTCCGGTT+CGG + chr4.2:35975973-35975992 None:intergenic 65.0%
GCCGAACCGGAACCTAGACC+GGG - chr4.2:35975969-35975988 MS.gene062918:intron 65.0%
GGCCGAACCGGAACCTAGAC+CGG - chr4.2:35975968-35975987 MS.gene062918:intron 65.0%
GTTGGGCCCGGTCTAGGTTC+CGG + chr4.2:35975978-35975997 None:intergenic 65.0%
!! GCATTGGTTGGGCCCGGTCT+AGG + chr4.2:35975984-35976003 None:intergenic 65.0%
!! GTCTAGGTTCCGGTTCGGCC+CGG + chr4.2:35975968-35975987 None:intergenic 65.0%
!! TCTAGGTTCCGGTTCGGCCC+GGG + chr4.2:35975967-35975986 None:intergenic 65.0%
AGACTCGCCGTCCCCGGTAC+CGG + chr4.2:35975565-35975584 None:intergenic 70.0%
AGTTCAGGCCCGGGCCGAAC+CGG - chr4.2:35975956-35975975 MS.gene062918:intron 70.0%
GCCCCACCCGTTTGGGCTTC+GGG + chr4.2:35976125-35976144 None:intergenic 70.0%
GCCCGAAGCCCAAACGGGTG+GGG - chr4.2:35976121-35976140 MS.gene062918:CDS 70.0%
GGAGTCAGACTCGCCGTCCC+CGG + chr4.2:35975571-35975590 None:intergenic 70.0%
GGTTCGGCCCGGGCCTGAAC+TGG + chr4.2:35975957-35975976 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 35975261 35976412 35975261 ID=MS.gene062918
chr4.2 mRNA 35975261 35976412 35975261 ID=MS.gene062918.t1;Parent=MS.gene062918
chr4.2 exon 35976056 35976412 35976056 ID=MS.gene062918.t1.exon1;Parent=MS.gene062918.t1
chr4.2 CDS 35976056 35976412 35976056 ID=cds.MS.gene062918.t1;Parent=MS.gene062918.t1
chr4.2 exon 35975261 35975794 35975261 ID=MS.gene062918.t1.exon2;Parent=MS.gene062918.t1
chr4.2 CDS 35975261 35975794 35975261 ID=cds.MS.gene062918.t1;Parent=MS.gene062918.t1
Gene Sequence

>MS.gene062918

ATGGAAGTTGTTGTAGCGGAAGCGTTGAAACCAAGCTTGAGGACAGAATCTATTTTCCAACAAACAATTTGTGAAGAGATTTTGTGTTTGAACGCAAACAACGTTGTTGTCGGAGAAGATTTTTCTGTCGACGACTTGCTTGATTTTTCCAACGGTGAATTTCAACATGGTTCTGTTGGAAAAGAAATTGATGTTTACCAGGAGGAGGAAGAAGAAGAAGAACATGAGAAAAACAGCACCACCTCAGGTTCTGAACATGAACGAACAGAAGATGACAACAATTCAAATTCCACAACATTTTCCGGTACCGGGGACGGCGAGTCTGACTCCATTTTTGCCGGTGGTTTAGCAGTTCCGGTGAGTCTTTAACCATGCAATAAATTATTTTTGTTTTTTATTTAATAAAGAATGAATTCATTACCTTTGTAGCATGGACATTTCTGATAGAAGATGTGCTAGTATCCCACACATGTCACTAACACGACACATTTGATTACATTGAATTATGTTTTTTTTTTTTTATTATCACTAGTATCATGTGTCCGGTCGGTGTTTGTGTCGTGTCTGTATGTGCTTCATATATAACTAATAACTCGTTTTGGTTTTCTTAATTCTCAGGCCGATGATGTTGCGGATTTGGAATGGGTGTCTCACTTTGTGGACGATTCTGTACCAGAACTCTCTCTATTGTATCCAGTTCAGGCCCGGGCCGAACCGGAACCTAGACCGGGCCCAACCAATGCTTATTCCCAGCTCTCTATAATCCGCAGAAAGCCTAGAACAACCAAGACAAGAAGGCCCAACTGTAACGTGTGGATTTTTAACCCTATACTCTTTTCAGCAAAGAAGCAGAGGAAAAAGCCCGAAGCCCAAACGGGTGGGGCTCAGTTTCAACGACGGTGCAGCCACTGCCATGTTCAGAAAACGCCACAGTGGAGAGCTGGTCCGCTTGGACCGAAAACACTATGTAACGCTTGTGGTGTTCGGTTTAAGTCCGGTCGGCTTTTCCCTGAGTATAGACCTGCATGCAGCCCGACGTTTTCCGGTGAAATTCATTCGAACAGCCACCGTAAGGTTTTGGAAATGAGGCGGAGGAAGGAGGTGGATGAACCGGTTTCCGGTCTGAACCGGATTCAAATGGTCACGAGTTGA
Protein sequence

>MS.gene062918.t1

MEVVVAEALKPSLRTESIFQQTICEEILCLNANNVVVGEDFSVDDLLDFSNGEFQHGSVGKEIDVYQEEEEEEEHEKNSTTSGSEHERTEDDNNSNSTTFSGTGDGESDSIFAGGLAVPADDVADLEWVSHFVDDSVPELSLLYPVQARAEPEPRPGPTNAYSQLSIIRRKPRTTKTRRPNCNVWIFNPILFSAKKQRKKPEAQTGGAQFQRRCSHCHVQKTPQWRAGPLGPKTLCNACGVRFKSGRLFPEYRPACSPTFSGEIHSNSHRKVLEMRRRKEVDEPVSGLNRIQMVTS