AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063129

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063129.t1 MTR_3g117380 94.020 301 14 1 1 297 15 315 0.0 539
MS.gene063129.t1 MTR_4g071590 56.051 314 101 8 1 296 1 295 1.67e-101 299
MS.gene063129.t1 MTR_4g471590 56.051 314 101 8 1 296 1 295 1.67e-101 299
MS.gene063129.t1 MTR_8g076340 41.135 282 136 8 21 287 25 291 1.11e-53 177
MS.gene063129.t1 MTR_8g013950 37.868 272 130 7 39 280 33 295 6.87e-46 158
MS.gene063129.t1 MTR_3g109760 40.824 267 127 8 36 281 21 277 6.90e-46 157
MS.gene063129.t1 MTR_5g021340 39.198 324 152 9 1 282 51 371 8.95e-46 159
MS.gene063129.t1 MTR_1g006650 34.653 303 153 8 18 281 8 304 3.36e-43 151
MS.gene063129.t1 MTR_1g046680 62.637 91 34 0 190 280 151 241 3.72e-34 125
MS.gene063129.t1 MTR_1g116010 36.697 218 103 6 93 275 11 228 1.98e-33 123
MS.gene063129.t1 MTR_1g094930 76.923 65 15 0 211 275 147 211 2.99e-33 122
MS.gene063129.t1 MTR_1g094930 76.923 65 15 0 211 275 242 306 1.31e-32 123
MS.gene063129.t1 MTR_7g085410 37.288 236 105 7 80 275 20 252 1.71e-32 121
MS.gene063129.t1 MTR_6g032830 37.004 227 81 8 107 275 34 256 2.90e-32 120
MS.gene063129.t1 MTR_2g094600 68.493 73 23 0 203 275 208 280 5.12e-32 120
MS.gene063129.t1 MTR_4g058910 73.134 67 18 0 211 277 314 380 9.64e-31 119
MS.gene063129.t1 MTR_5g007600 62.025 79 30 0 202 280 250 328 2.89e-29 114
MS.gene063129.t1 MTR_5g007600 54.945 91 41 0 192 282 138 228 4.50e-27 108
MS.gene063129.t1 MTR_7g446110 58.730 63 26 0 210 272 186 248 3.19e-21 90.9
MS.gene063129.t1 MTR_7g445950 55.556 63 28 0 210 272 119 181 9.08e-20 85.1
MS.gene063129.t1 MTR_7g446070 31.844 179 107 6 100 276 30 195 1.61e-19 84.7
MS.gene063129.t1 MTR_7g445980 56.923 65 27 1 209 272 31 95 1.62e-19 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063129.t1 AT4G36240 43.426 251 101 7 40 278 10 231 1.01e-52 172
MS.gene063129.t1 AT5G66320 40.000 315 142 10 8 278 5 316 2.20e-49 167
MS.gene063129.t1 AT5G66320 40.000 315 142 10 8 278 5 316 2.20e-49 167
MS.gene063129.t1 AT4G32890 40.426 282 129 14 21 280 2 266 6.83e-47 160
MS.gene063129.t1 AT4G34680 41.958 286 112 10 6 280 7 249 3.26e-46 157
MS.gene063129.t1 AT4G34680 41.958 286 112 10 6 280 7 249 3.26e-46 157
MS.gene063129.t1 AT5G25830 38.596 285 122 13 40 280 13 288 7.96e-42 147
MS.gene063129.t1 AT3G60530 46.277 188 73 5 94 278 63 225 1.68e-41 144
MS.gene063129.t1 AT2G45050 40.000 210 93 5 87 281 58 249 1.15e-38 137
MS.gene063129.t1 AT3G51080 82.353 68 12 0 211 278 221 288 3.36e-36 132
MS.gene063129.t1 AT3G54810 73.529 68 18 0 211 278 229 296 5.15e-32 121
MS.gene063129.t1 AT3G54810 73.529 68 18 0 211 278 229 296 5.15e-32 121
MS.gene063129.t1 AT3G24050 65.476 84 28 1 194 277 178 260 5.26e-32 120
MS.gene063129.t1 AT1G08010 63.636 77 28 0 201 277 210 286 4.66e-31 118
MS.gene063129.t1 AT1G08010 63.636 77 28 0 201 277 210 286 4.66e-31 118
MS.gene063129.t1 AT1G08010 63.636 77 28 0 201 277 210 286 4.66e-31 118
MS.gene063129.t1 AT1G08000 56.383 94 34 1 191 277 191 284 1.29e-29 114
MS.gene063129.t1 AT1G08000 56.383 94 34 1 191 277 191 284 1.29e-29 114
MS.gene063129.t1 AT3G45170 55.422 83 37 0 196 278 100 182 2.33e-27 105
MS.gene063129.t1 AT2G28340 61.538 65 25 0 213 277 193 257 6.19e-25 101

Find 82 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGTAACTGACCCTCTTCT+TGG 0.222887 3.4:-99770097 None:intergenic
GAGTAATTCGGTTGAGAAAA+TGG 0.282779 3.4:-99769683 None:intergenic
TTCTCATCTCCAACACTTTA+CGG 0.294944 3.4:-99770292 None:intergenic
CAACACTTTACGGTGGCTAT+TGG 0.312622 3.4:-99770282 None:intergenic
CTCAGCAAGTGGTAACCATT+TGG 0.349550 3.4:-99770361 None:intergenic
GAAAACCCAACCCTCGCGTT+TGG 0.352223 3.4:+99769985 MS.gene063129:CDS
ACCGCCAACACGGTGGTTGC+CGG 0.362678 3.4:+99769499 MS.gene063129:CDS
TTCTTGGGCCTCAAGAGCCT+CGG 0.380431 3.4:-99770081 None:intergenic
GGATGATGACGTGGCAGGTC+TGG 0.383333 3.4:+99769791 MS.gene063129:intron
TGGACCGCAACCCGGTTTAC+CGG 0.387691 3.4:+99770331 MS.gene063129:CDS
TTGGGTCTGAGCCGGTAAAC+CGG 0.389320 3.4:-99770342 None:intergenic
TGAGCCCAAACGCGAGGGTT+GGG 0.405637 3.4:-99769990 None:intergenic
AAAACCCAACCCTCGCGTTT+GGG 0.407277 3.4:+99769986 MS.gene063129:CDS
GTGTTGGTTTGAATTTGAAC+CGG 0.408949 3.4:-99769867 None:intergenic
TGAATTTCACTGCTGAAAGT+TGG 0.418828 3.4:-99770257 None:intergenic
TTGTAACTGACCCTCTTCTT+GGG 0.419497 3.4:-99770096 None:intergenic
GGGTAGAGAAGAGATAGTTC+TGG 0.431245 3.4:-99769846 None:intergenic
TCAGCAAGTGGTAACCATTT+GGG 0.434470 3.4:-99770360 None:intergenic
GAATTTCACTGCTGAAAGTT+GGG 0.435175 3.4:-99770256 None:intergenic
ACTGCTGAAAGTTGGGCTAC+AGG 0.440191 3.4:-99770249 None:intergenic
TGCGGGGTTCGTTATAAATC+AGG 0.450021 3.4:+99770200 MS.gene063129:CDS
ACTCACGGGAACGAGTAATT+CGG 0.455413 3.4:-99769695 None:intergenic
CTGAGCCCAAACGCGAGGGT+TGG 0.457925 3.4:-99769991 None:intergenic
CAGTGGAGAACCGGTCCGTT+GGG 0.465140 3.4:+99770155 MS.gene063129:CDS
AAGACGCCGCAGTGGAGAAC+CGG 0.467079 3.4:+99770146 MS.gene063129:CDS
CTTTGAAACCGAGTTTAAGA+AGG 0.468143 3.4:+99769431 MS.gene063129:CDS
AAACCCTTTGTAACGCGTGC+GGG 0.468327 3.4:+99770183 MS.gene063129:CDS
ACCGGCAACCACCGTGTTGG+CGG 0.469757 3.4:-99769500 None:intergenic
CTTCACGGTTCTGGTCTTCG+TGG 0.473101 3.4:-99769958 None:intergenic
TCTTGGGCCTCAAGAGCCTC+GGG 0.483424 3.4:-99770080 None:intergenic
GGTAGAGAAGAGATAGTTCT+GGG 0.490371 3.4:-99769845 None:intergenic
TTCTTCTGTTTCTTCGCTGG+TGG 0.490862 3.4:-99770053 None:intergenic
ACTGCGGCGTCTTCTGCACC+TGG 0.496651 3.4:-99770136 None:intergenic
GCAGTGGAGAACCGGTCCGT+TGG 0.505231 3.4:+99770154 MS.gene063129:CDS
CTCCTTGACTTCTCTAACGG+CGG 0.512576 3.4:+99769544 MS.gene063129:CDS
ATGACGTGGCAGGTCTGGAA+TGG 0.521490 3.4:+99769796 MS.gene063129:CDS
AACCCCGCACGCGTTACAAA+GGG 0.526943 3.4:-99770186 None:intergenic
TGAGAAAATGGAAGAATCAA+AGG 0.527539 3.4:-99769671 None:intergenic
AAGTGTTGGAGATGAGAAAG+AGG 0.539631 3.4:+99770297 MS.gene063129:CDS
ACGACGGTGTAGTCATTGCC+AGG 0.543797 3.4:+99770118 MS.gene063129:CDS
CTGAAAGTTGGGCTACAGGC+CGG 0.545068 3.4:-99770245 None:intergenic
TGGGTCTGAGCCGGTAAACC+GGG 0.548677 3.4:-99770341 None:intergenic
AACCCTTTGTAACGCGTGCG+GGG 0.556096 3.4:+99770184 MS.gene063129:CDS
CAATAGCCACCGTAAAGTGT+TGG 0.557110 3.4:+99770283 MS.gene063129:CDS
AGAAAGAGGAAAGGAATGGT+TGG 0.560194 3.4:+99770311 MS.gene063129:CDS
ACAAACTCCCTTCTTAAACT+CGG 0.560314 3.4:-99769439 None:intergenic
GAACCCCGCACGCGTTACAA+AGG 0.560484 3.4:-99770187 None:intergenic
TTACAGGATGATGACGTGGC+AGG 0.560919 3.4:+99769786 MS.gene063129:intron
TGAGCCGGTAAACCGGGTTG+CGG 0.561596 3.4:-99770335 None:intergenic
GCTCTTGAGGCCCAAGAAGA+GGG 0.561687 3.4:+99770086 MS.gene063129:CDS
GTAACCATTTGGGTCTGAGC+CGG 0.562666 3.4:-99770350 None:intergenic
TTGGAGATGAGAAAGAGGAA+AGG 0.563009 3.4:+99770302 MS.gene063129:CDS
GGGCGCTGAGCCCAAACGCG+AGG 0.563588 3.4:-99769996 None:intergenic
TTTGAAACCGAGTTTAAGAA+GGG 0.567414 3.4:+99769432 MS.gene063129:CDS
GGCTCTTGAGGCCCAAGAAG+AGG 0.567428 3.4:+99770085 MS.gene063129:CDS
GAAGTCAAGGAGGTCGTCAA+CGG 0.569324 3.4:-99769533 None:intergenic
CACCGCCGTTAGAGAAGTCA+AGG 0.571693 3.4:-99769546 None:intergenic
ATCTTCTGAGAGTGATTGAG+AGG 0.572536 3.4:-99769623 None:intergenic
TTTACCGGCTCAGACCCAAA+TGG 0.575540 3.4:+99770346 MS.gene063129:CDS
TGTTGGTTTGAATTTGAACC+GGG 0.578403 3.4:-99769866 None:intergenic
GATGAGAAAGAGGAAAGGAA+TGG 0.578460 3.4:+99770307 MS.gene063129:CDS
TGACGTGGCAGGTCTGGAAT+GGG 0.589105 3.4:+99769797 MS.gene063129:CDS
GGAATGGTTGGACCGCAACC+CGG 0.596301 3.4:+99770323 MS.gene063129:CDS
TCTCTTCTTCATCTCCGTCG+TGG 0.602523 3.4:-99769579 None:intergenic
AGAATCAAAGGAAGCGTCGG+TGG 0.602647 3.4:-99769659 None:intergenic
GGCGCTGAGCCCAAACGCGA+GGG 0.608307 3.4:-99769995 None:intergenic
GGAAGAATCAAAGGAAGCGT+CGG 0.616525 3.4:-99769662 None:intergenic
TTCACCGGCAACCACCGTGT+TGG 0.618211 3.4:-99769503 None:intergenic
CAATTTACAGGATGATGACG+TGG 0.618285 3.4:+99769782 MS.gene063129:intron
CTAATGAATGACTCAGCAAG+TGG 0.621278 3.4:-99770372 None:intergenic
AGGTGCAGAAGACGCCGCAG+TGG 0.632590 3.4:+99770138 MS.gene063129:CDS
AGAACTATCTCTTCTCTACC+CGG 0.632934 3.4:+99769848 MS.gene063129:CDS
GGGTTCGTTATAAATCAGGT+CGG 0.635663 3.4:+99770204 MS.gene063129:CDS
GTCAACGGAGAAATCTTCAC+CGG 0.637583 3.4:-99769518 None:intergenic
GACCTCCTTGACTTCTCTAA+CGG 0.644071 3.4:+99769541 MS.gene063129:CDS
AAAACCCTTTGTAACGCGTG+CGG 0.650760 3.4:+99770182 MS.gene063129:CDS
TCATCTCCAACACTTTACGG+TGG 0.668782 3.4:-99770289 None:intergenic
AACGGACCGGTTCTCCACTG+CGG 0.699688 3.4:-99770152 None:intergenic
TAGCTTCACCGCCAACACGG+TGG 0.733444 3.4:+99769492 MS.gene063129:CDS
CGCCGTTAGAGAAGTCAAGG+AGG 0.737377 3.4:-99769543 None:intergenic
AAGAGGGTCAGTTACAACGA+CGG 0.739560 3.4:+99770102 MS.gene063129:CDS
GGTGATATTCTTCACCACGA+CGG 0.777931 3.4:+99769565 MS.gene063129:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGTAATTGAAATTGAAATT+GGG + chr3.4:99769755-99769774 MS.gene063129:intron 15.0%
!! TTAGTAATTGAAATTGAAAT+TGG + chr3.4:99769754-99769773 MS.gene063129:intron 15.0%
!! TTTCAATTACTAATACAAAA+CGG - chr3.4:99769747-99769766 None:intergenic 15.0%
!! AGTAATTGAAATTGAAATTG+GGG + chr3.4:99769756-99769775 MS.gene063129:intron 20.0%
! GTAATTGAAATTGAAATTGG+GGG + chr3.4:99769757-99769776 MS.gene063129:intron 25.0%
TGAGAAAATGGAAGAATCAA+AGG - chr3.4:99769674-99769693 None:intergenic 30.0%
! TTTGAAACCGAGTTTAAGAA+GGG + chr3.4:99769432-99769451 MS.gene063129:CDS 30.0%
!! TTTGTTTTTCAGCAAACTCT+CGG + chr3.4:99769457-99769476 MS.gene063129:CDS 30.0%
AAATTGGGGGTTCAATTTAC+AGG + chr3.4:99769770-99769789 MS.gene063129:intron 35.0%
AACGAAGAGAGAAAAACTCA+CGG - chr3.4:99769713-99769732 None:intergenic 35.0%
ACAAACTCCCTTCTTAAACT+CGG - chr3.4:99769442-99769461 None:intergenic 35.0%
CAAAAACATGAAACTCAGCA+TGG + chr3.4:99769897-99769916 MS.gene063129:CDS 35.0%
GAATTTCACTGCTGAAAGTT+GGG - chr3.4:99770259-99770278 None:intergenic 35.0%
GAGTAATTCGGTTGAGAAAA+TGG - chr3.4:99769686-99769705 None:intergenic 35.0%
TGAATTTCACTGCTGAAAGT+TGG - chr3.4:99770260-99770279 None:intergenic 35.0%
TTCTCATCTCCAACACTTTA+CGG - chr3.4:99770295-99770314 None:intergenic 35.0%
! GTTGGGTTTTCTATTCTTCA+CGG - chr3.4:99769976-99769995 None:intergenic 35.0%
! TTTTCTATTCTTCACGGTTC+TGG - chr3.4:99769970-99769989 None:intergenic 35.0%
!! CTTTGAAACCGAGTTTAAGA+AGG + chr3.4:99769431-99769450 MS.gene063129:CDS 35.0%
!! GTGTTGGTTTGAATTTGAAC+CGG - chr3.4:99769870-99769889 None:intergenic 35.0%
!! TGCTGAGTTTCATGTTTTTG+AGG - chr3.4:99769897-99769916 None:intergenic 35.0%
!! TGTTGGTTTGAATTTGAACC+GGG - chr3.4:99769869-99769888 None:intergenic 35.0%
!! TTTTTCTTCTGTTTCTTCGC+TGG - chr3.4:99770059-99770078 None:intergenic 35.0%
!!! TAAATCAGGTCGGCTTTTTT+CGG + chr3.4:99770214-99770233 MS.gene063129:CDS 35.0%
ACGAAGAGAGAAAAACTCAC+GGG - chr3.4:99769712-99769731 None:intergenic 40.0%
AGAAAGAGGAAAGGAATGGT+TGG + chr3.4:99770311-99770330 MS.gene063129:CDS 40.0%
AGAACTATCTCTTCTCTACC+CGG + chr3.4:99769848-99769867 MS.gene063129:CDS 40.0%
GATGAGAAAGAGGAAAGGAA+TGG + chr3.4:99770307-99770326 MS.gene063129:CDS 40.0%
GGGTTCGTTATAAATCAGGT+CGG + chr3.4:99770204-99770223 MS.gene063129:CDS 40.0%
GGTAGAGAAGAGATAGTTCT+GGG - chr3.4:99769848-99769867 None:intergenic 40.0%
TCAGCAAGTGGTAACCATTT+GGG - chr3.4:99770363-99770382 None:intergenic 40.0%
TTGGAGATGAGAAAGAGGAA+AGG + chr3.4:99770302-99770321 MS.gene063129:CDS 40.0%
TTGTAACTGACCCTCTTCTT+GGG - chr3.4:99770099-99770118 None:intergenic 40.0%
! ATCTTCTGAGAGTGATTGAG+AGG - chr3.4:99769626-99769645 None:intergenic 40.0%
! CAATTTACAGGATGATGACG+TGG + chr3.4:99769782-99769801 MS.gene063129:intron 40.0%
!! AAGTGTTGGAGATGAGAAAG+AGG + chr3.4:99770297-99770316 MS.gene063129:CDS 40.0%
!! ATGTTTTTGAGGACACGTGT+TGG - chr3.4:99769886-99769905 None:intergenic 40.0%
!!! GCTTTTTTCGGAGTATAGAC+CGG + chr3.4:99770226-99770245 MS.gene063129:CDS 40.0%
AAAACCCTTTGTAACGCGTG+CGG + chr3.4:99770182-99770201 MS.gene063129:CDS 45.0%
AAGAGGGTCAGTTACAACGA+CGG + chr3.4:99770102-99770121 MS.gene063129:CDS 45.0%
ACTCACGGGAACGAGTAATT+CGG - chr3.4:99769698-99769717 None:intergenic 45.0%
CAACACTTTACGGTGGCTAT+TGG - chr3.4:99770285-99770304 None:intergenic 45.0%
CAATAGCCACCGTAAAGTGT+TGG + chr3.4:99770283-99770302 MS.gene063129:CDS 45.0%
CCAAAAACAACAGACGGTGA+CGG - chr3.4:99770029-99770048 None:intergenic 45.0%
GAAACAGAAGAAAAAGCCCG+AGG + chr3.4:99770064-99770083 MS.gene063129:CDS 45.0%
GACCTCCTTGACTTCTCTAA+CGG + chr3.4:99769541-99769560 MS.gene063129:CDS 45.0%
GGGTAGAGAAGAGATAGTTC+TGG - chr3.4:99769849-99769868 None:intergenic 45.0%
GGTGATATTCTTCACCACGA+CGG + chr3.4:99769565-99769584 MS.gene063129:CDS 45.0%
GTCAACGGAGAAATCTTCAC+CGG - chr3.4:99769521-99769540 None:intergenic 45.0%
GTTGTAACTGACCCTCTTCT+TGG - chr3.4:99770100-99770119 None:intergenic 45.0%
TCATCTCCAACACTTTACGG+TGG - chr3.4:99770292-99770311 None:intergenic 45.0%
TGCGGGGTTCGTTATAAATC+AGG + chr3.4:99770200-99770219 MS.gene063129:CDS 45.0%
! GGAAGAATCAAAGGAAGCGT+CGG - chr3.4:99769665-99769684 None:intergenic 45.0%
! TTCTTCTGTTTCTTCGCTGG+TGG - chr3.4:99770056-99770075 None:intergenic 45.0%
!! ACAGACGGTGACGGAAAAAA+CGG - chr3.4:99770020-99770039 None:intergenic 45.0%
!! CAAAGGGTTTTAGCACCCAA+CGG - chr3.4:99770173-99770192 None:intergenic 45.0%
!!! CGTCACCGTCTGTTGTTTTT+GGG + chr3.4:99770027-99770046 MS.gene063129:CDS 45.0%
!!! GTCACCGTCTGTTGTTTTTG+GGG + chr3.4:99770028-99770047 MS.gene063129:CDS 45.0%
!!! TCACCGTCTGTTGTTTTTGG+GGG + chr3.4:99770029-99770048 MS.gene063129:CDS 45.0%
!!! TTTTAGCTTCACCGCCAACA+CGG + chr3.4:99769489-99769508 MS.gene063129:CDS 45.0%
AAAACCCAACCCTCGCGTTT+GGG + chr3.4:99769986-99770005 MS.gene063129:CDS 50.0%
AAACCCTTTGTAACGCGTGC+GGG + chr3.4:99770183-99770202 MS.gene063129:CDS 50.0%
ACTGCTGAAAGTTGGGCTAC+AGG - chr3.4:99770252-99770271 None:intergenic 50.0%
CTCCTTGACTTCTCTAACGG+CGG + chr3.4:99769544-99769563 MS.gene063129:CDS 50.0%
GAAGTCAAGGAGGTCGTCAA+CGG - chr3.4:99769536-99769555 None:intergenic 50.0%
GGTCCCCCAAAAACAACAGA+CGG - chr3.4:99770035-99770054 None:intergenic 50.0%
GTAACCATTTGGGTCTGAGC+CGG - chr3.4:99770353-99770372 None:intergenic 50.0%
TCTCTTCTTCATCTCCGTCG+TGG - chr3.4:99769582-99769601 None:intergenic 50.0%
! TTACAGGATGATGACGTGGC+AGG + chr3.4:99769786-99769805 MS.gene063129:intron 50.0%
! TTTTCAGCAAACTCTCGGCG+AGG + chr3.4:99769462-99769481 MS.gene063129:CDS 50.0%
!! AGAATCAAAGGAAGCGTCGG+TGG - chr3.4:99769662-99769681 None:intergenic 50.0%
!! CAGACGGTGACGGAAAAAAC+GGG - chr3.4:99770019-99770038 None:intergenic 50.0%
!! TTTACCGGCTCAGACCCAAA+TGG + chr3.4:99770346-99770365 MS.gene063129:CDS 50.0%
!!! CCGTCACCGTCTGTTGTTTT+TGG + chr3.4:99770026-99770045 MS.gene063129:CDS 50.0%
AACCCCGCACGCGTTACAAA+GGG - chr3.4:99770189-99770208 None:intergenic 55.0%
AACCCTTTGTAACGCGTGCG+GGG + chr3.4:99770184-99770203 MS.gene063129:CDS 55.0%
ACGACGGTGTAGTCATTGCC+AGG + chr3.4:99770118-99770137 MS.gene063129:CDS 55.0%
ATGACGTGGCAGGTCTGGAA+TGG + chr3.4:99769796-99769815 MS.gene063129:CDS 55.0%
CACCGCCGTTAGAGAAGTCA+AGG - chr3.4:99769549-99769568 None:intergenic 55.0%
CGCCGTTAGAGAAGTCAAGG+AGG - chr3.4:99769546-99769565 None:intergenic 55.0%
CTGAAAGTTGGGCTACAGGC+CGG - chr3.4:99770248-99770267 None:intergenic 55.0%
GAAAAAGCCCGAGGCTCTTG+AGG + chr3.4:99770073-99770092 MS.gene063129:CDS 55.0%
GAAAACCCAACCCTCGCGTT+TGG + chr3.4:99769985-99770004 MS.gene063129:CDS 55.0%
GCTCTTGAGGCCCAAGAAGA+GGG + chr3.4:99770086-99770105 MS.gene063129:CDS 55.0%
TTCTTGGGCCTCAAGAGCCT+CGG - chr3.4:99770084-99770103 None:intergenic 55.0%
TTGGGTCTGAGCCGGTAAAC+CGG - chr3.4:99770345-99770364 None:intergenic 55.0%
! CTTCACGGTTCTGGTCTTCG+TGG - chr3.4:99769961-99769980 None:intergenic 55.0%
! TGACGTGGCAGGTCTGGAAT+GGG + chr3.4:99769797-99769816 MS.gene063129:CDS 55.0%
!! GGTTTTAGCACCCAACGGAC+CGG - chr3.4:99770168-99770187 None:intergenic 55.0%
AAGACGCCGCAGTGGAGAAC+CGG + chr3.4:99770146-99770165 MS.gene063129:CDS 60.0%
CAGTGGAGAACCGGTCCGTT+GGG + chr3.4:99770155-99770174 MS.gene063129:CDS 60.0%
GAACCCCGCACGCGTTACAA+AGG - chr3.4:99770190-99770209 None:intergenic 60.0%
GGAATGGTTGGACCGCAACC+CGG + chr3.4:99770323-99770342 MS.gene063129:CDS 60.0%
GGCTCTTGAGGCCCAAGAAG+AGG + chr3.4:99770085-99770104 MS.gene063129:CDS 60.0%
TAGCTTCACCGCCAACACGG+TGG + chr3.4:99769492-99769511 MS.gene063129:CDS 60.0%
TCTTGGGCCTCAAGAGCCTC+GGG - chr3.4:99770083-99770102 None:intergenic 60.0%
TGAGCCCAAACGCGAGGGTT+GGG - chr3.4:99769993-99770012 None:intergenic 60.0%
TGAGCCGGTAAACCGGGTTG+CGG - chr3.4:99770338-99770357 None:intergenic 60.0%
TGGACCGCAACCCGGTTTAC+CGG + chr3.4:99770331-99770350 MS.gene063129:CDS 60.0%
TGGGTCTGAGCCGGTAAACC+GGG - chr3.4:99770344-99770363 None:intergenic 60.0%
TTCACCGGCAACCACCGTGT+TGG - chr3.4:99769506-99769525 None:intergenic 60.0%
! AACGGACCGGTTCTCCACTG+CGG - chr3.4:99770155-99770174 None:intergenic 60.0%
! GGATGATGACGTGGCAGGTC+TGG + chr3.4:99769791-99769810 MS.gene063129:intron 60.0%
ACCGCCAACACGGTGGTTGC+CGG + chr3.4:99769499-99769518 MS.gene063129:CDS 65.0%
ACCGGCAACCACCGTGTTGG+CGG - chr3.4:99769503-99769522 None:intergenic 65.0%
ACTGCGGCGTCTTCTGCACC+TGG - chr3.4:99770139-99770158 None:intergenic 65.0%
AGGTGCAGAAGACGCCGCAG+TGG + chr3.4:99770138-99770157 MS.gene063129:CDS 65.0%
CTGAGCCCAAACGCGAGGGT+TGG - chr3.4:99769994-99770013 None:intergenic 65.0%
GCAGTGGAGAACCGGTCCGT+TGG + chr3.4:99770154-99770173 MS.gene063129:CDS 65.0%
! GGCGCTGAGCCCAAACGCGA+GGG - chr3.4:99769998-99770017 None:intergenic 70.0%
!! GGGCGCTGAGCCCAAACGCG+AGG - chr3.4:99769999-99770018 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 99769409 99770382 99769409 ID=MS.gene063129
chr3.4 mRNA 99769409 99770382 99769409 ID=MS.gene063129.t1;Parent=MS.gene063129
chr3.4 exon 99769409 99769711 99769409 ID=MS.gene063129.t1.exon1;Parent=MS.gene063129.t1
chr3.4 CDS 99769409 99769711 99769409 ID=cds.MS.gene063129.t1;Parent=MS.gene063129.t1
chr3.4 exon 99769792 99770382 99769792 ID=MS.gene063129.t1.exon2;Parent=MS.gene063129.t1
chr3.4 CDS 99769792 99770382 99769792 ID=cds.MS.gene063129.t1;Parent=MS.gene063129.t1
Gene Sequence

>MS.gene063129

ATGGAGTTTGCAGTAGCAAAAGCTTTGAAACCGAGTTTAAGAAGGGAGTTTGTTTTTCAGCAAACTCTCGGCGAGGAACTTTTTAGCTTCACCGCCAACACGGTGGTTGCCGGTGAAGATTTCTCCGTTGACGACCTCCTTGACTTCTCTAACGGCGGTGATATTCTTCACCACGACGGAGATGAAGAAGAGAAAGATAGCAGTTTATCTCTTTCCTCTCAATCACTCTCAGAAGATAACAACTCAAATTCCACCGACGCTTCCTTTGATTCTTCCATTTTCTCAACCGAATTACTCGTTCCCGTGAGTTTTTCTCTCTTCGTTTCTTTTTCTCTCCGTTTTGTATTAGTAATTGAAATTGAAATTGGGGGTTCAATTTACAGGATGATGACGTGGCAGGTCTGGAATGGGTTTCACACTTTGTAGATGATTCTCTCCCAGAACTATCTCTTCTCTACCCGGTTCAAATTCAAACCAACACGTGTCCTCAAAAACATGAAACTCAGCATGGAAAAACTTTACGTTTTTCAACTGAAAAAATGAAAATTACCACGAAGACCAGAACCGTGAAGAATAGAAAACCCAACCCTCGCGTTTGGGCTCAGCGCCCGTTTTTTCCGTCACCGTCTGTTGTTTTTGGGGGACCACCAGCGAAGAAACAGAAGAAAAAGCCCGAGGCTCTTGAGGCCCAAGAAGAGGGTCAGTTACAACGACGGTGTAGTCATTGCCAGGTGCAGAAGACGCCGCAGTGGAGAACCGGTCCGTTGGGTGCTAAAACCCTTTGTAACGCGTGCGGGGTTCGTTATAAATCAGGTCGGCTTTTTTCGGAGTATAGACCGGCCTGTAGCCCAACTTTCAGCAGTGAAATTCATTCCAATAGCCACCGTAAAGTGTTGGAGATGAGAAAGAGGAAAGGAATGGTTGGACCGCAACCCGGTTTACCGGCTCAGACCCAAATGGTTACCACTTGCTGA
Protein sequence

>MS.gene063129.t1

MEFAVAKALKPSLRREFVFQQTLGEELFSFTANTVVAGEDFSVDDLLDFSNGGDILHHDGDEEEKDSSLSLSSQSLSEDNNSNSTDASFDSSIFSTELLVPDDDVAGLEWVSHFVDDSLPELSLLYPVQIQTNTCPQKHETQHGKTLRFSTEKMKITTKTRTVKNRKPNPRVWAQRPFFPSPSVVFGGPPAKKQKKKPEALEAQEEGQLQRRCSHCQVQKTPQWRTGPLGAKTLCNACGVRYKSGRLFSEYRPACSPTFSSEIHSNSHRKVLEMRKRKGMVGPQPGLPAQTQMVTTC