AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063201

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063201.t1 MTR_1g075610 97.857 140 3 0 1 140 1 140 5.06e-94 278
MS.gene063201.t1 MTR_4g007750 58.209 134 47 2 16 140 2 135 1.29e-43 149
MS.gene063201.t1 MTR_4g094430 47.200 125 58 3 16 140 2 118 3.89e-30 113
MS.gene063201.t1 MTR_2g032060 38.596 171 87 6 16 176 2 164 7.86e-29 109
MS.gene063201.t1 MTR_3g040520 41.912 136 64 4 16 140 23 154 4.39e-20 88.2
MS.gene063201.t1 MTR_3g040810 40.441 136 66 4 16 140 21 152 1.22e-19 87.0
MS.gene063201.t1 MTR_8g080190 37.410 139 78 4 3 139 368 499 4.75e-19 85.5
MS.gene063201.t1 MTR_8g092290 38.636 132 74 3 9 140 132 256 1.48e-18 84.0
MS.gene063201.t1 MTR_1g098300 36.232 138 80 4 4 140 111 241 7.18e-18 82.0
MS.gene063201.t1 MTR_8g461270 38.095 126 73 3 16 140 45 166 4.16e-17 79.0
MS.gene063201.t1 MTR_8g080190 40.323 124 67 3 16 139 383 499 6.83e-17 79.3
MS.gene063201.t1 MTR_3g085650 40.323 124 67 3 16 139 414 530 7.48e-17 79.0
MS.gene063201.t1 MTR_3g085650 40.323 124 67 3 16 139 414 530 1.06e-16 78.6
MS.gene063201.t1 MTR_3g085650 40.323 124 67 3 16 139 414 530 1.10e-16 78.6
MS.gene063201.t1 MTR_3g085650 40.323 124 67 3 16 139 414 530 1.33e-16 78.6
MS.gene063201.t1 MTR_3g054080 41.085 129 61 5 16 140 12 129 2.42e-16 77.4
MS.gene063201.t1 MTR_3g096960 35.659 129 75 4 16 140 16 140 8.21e-16 75.9
MS.gene063201.t1 MTR_8g099770 40.650 123 66 3 18 140 90 205 9.91e-16 75.9
MS.gene063201.t1 MTR_4g103810 40.164 122 68 3 18 139 108 224 1.80e-15 74.7
MS.gene063201.t1 MTR_4g103810 40.164 122 68 3 18 139 218 334 2.51e-15 74.7
MS.gene063201.t1 MTR_5g008860 39.200 125 69 3 16 140 138 255 3.32e-15 74.3
MS.gene063201.t1 MTR_6g080470 40.157 127 63 4 16 140 12 127 6.05e-15 73.2
MS.gene063201.t1 MTR_4g078290 35.433 127 75 3 14 140 125 244 8.87e-15 73.2
MS.gene063201.t1 MTR_4g078290 35.433 127 75 3 14 140 125 244 8.87e-15 73.2
MS.gene063201.t1 MTR_2g085200 38.400 125 70 3 16 140 132 249 9.31e-15 73.2
MS.gene063201.t1 MTR_2g020210 38.686 137 76 4 5 140 88 217 1.16e-14 72.8
MS.gene063201.t1 MTR_2g026860 66.038 53 15 1 84 136 5 54 1.82e-14 67.4
MS.gene063201.t1 MTR_3g098760 39.683 126 65 4 17 140 96 212 2.43e-14 71.6
MS.gene063201.t1 MTR_7g011800 45.192 104 52 3 5 107 106 205 7.48e-14 70.5
MS.gene063201.t1 MTR_2g090405 36.220 127 74 3 14 140 137 256 1.12e-13 69.7
MS.gene063201.t1 MTR_4g109090 37.984 129 65 4 16 140 103 220 1.22e-13 69.7
MS.gene063201.t1 MTR_6g013030 36.000 125 73 3 16 140 108 225 2.74e-13 68.6
MS.gene063201.t1 MTR_1g081870 38.400 125 70 3 16 140 120 237 3.09e-13 68.6
MS.gene063201.t1 MTR_4g118780 35.766 137 80 4 5 140 90 219 1.29e-12 66.6
MS.gene063201.t1 MTR_7g114300 37.795 127 68 4 16 140 105 222 1.50e-12 66.2
MS.gene063201.t1 MTR_7g114300 39.062 128 65 5 16 140 105 222 1.74e-12 66.2
MS.gene063201.t1 MTR_7g114300 37.795 127 68 4 16 140 105 222 1.88e-12 65.9
MS.gene063201.t1 MTR_7g114300 37.405 131 63 6 16 140 105 222 4.38e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063201.t1 AT1G76540 83.206 131 22 0 10 140 6 136 1.02e-73 226
MS.gene063201.t1 AT1G20930 82.707 133 23 0 8 140 6 138 1.05e-73 226
MS.gene063201.t1 AT3G54180 60.606 132 45 2 16 140 2 133 2.99e-43 147
MS.gene063201.t1 AT2G38620 57.778 135 46 3 16 140 2 135 9.27e-41 140
MS.gene063201.t1 AT2G38620 57.778 135 46 3 16 140 2 135 9.27e-41 140
MS.gene063201.t1 AT2G38620 57.778 135 46 3 16 140 2 135 1.00e-39 139
MS.gene063201.t1 AT3G48750 38.012 171 88 6 16 176 2 164 2.49e-29 111
MS.gene063201.t1 AT1G67580 40.323 124 67 3 16 139 404 520 2.55e-22 95.1
MS.gene063201.t1 AT1G67580 40.323 124 67 3 16 139 404 520 2.55e-22 95.1
MS.gene063201.t1 AT5G10270 41.912 136 64 4 16 140 24 155 2.82e-20 89.0
MS.gene063201.t1 AT5G64960 41.912 136 64 4 16 140 24 155 5.10e-20 88.2
MS.gene063201.t1 AT1G66750 40.845 142 66 5 1 140 1 126 8.62e-19 83.6
MS.gene063201.t1 AT1G18040 41.322 121 62 4 20 140 14 125 1.41e-16 77.8
MS.gene063201.t1 AT1G73690 38.843 121 65 4 20 140 13 124 1.92e-16 77.4
MS.gene063201.t1 AT1G71530 41.600 125 66 3 16 140 145 262 2.21e-16 77.0
MS.gene063201.t1 AT1G71530 41.600 125 66 3 16 140 145 262 2.97e-16 77.0
MS.gene063201.t1 AT1G71530 41.600 125 66 3 16 140 145 262 4.75e-16 76.6
MS.gene063201.t1 AT1G18670 40.000 125 68 3 16 140 129 246 6.13e-16 76.6
MS.gene063201.t1 AT1G18670 40.000 125 68 3 16 140 129 246 6.59e-16 76.3
MS.gene063201.t1 AT1G18670 40.000 125 68 3 16 140 129 246 7.04e-16 76.3
MS.gene063201.t1 AT1G03740 40.310 129 58 4 18 140 213 328 1.20e-15 75.5
MS.gene063201.t1 AT1G03740 40.310 129 58 4 18 140 213 328 1.22e-15 75.5
MS.gene063201.t1 AT1G71530 41.600 125 66 3 16 140 145 262 1.53e-15 75.5
MS.gene063201.t1 AT1G57700 38.760 129 68 4 14 140 142 261 3.54e-15 74.3
MS.gene063201.t1 AT5G39420 38.760 129 64 5 16 140 103 220 7.00e-15 73.6
MS.gene063201.t1 AT5G63610 35.433 127 76 4 16 140 23 145 2.41e-14 71.6
MS.gene063201.t1 AT4G10010 40.800 125 67 3 16 140 154 271 2.72e-14 71.6
MS.gene063201.t1 AT5G63370 35.714 126 71 4 18 139 163 282 2.87e-14 71.2
MS.gene063201.t1 AT1G33770 40.000 125 68 3 16 140 139 256 3.33e-14 71.2
MS.gene063201.t1 AT5G63370 35.714 126 71 4 18 139 163 282 3.90e-14 70.9
MS.gene063201.t1 AT5G63370 35.714 126 71 4 18 139 163 282 3.90e-14 70.9
MS.gene063201.t1 AT1G09600 38.400 125 70 3 16 140 161 278 4.08e-14 71.2
MS.gene063201.t1 AT5G63370 35.714 126 71 4 18 139 297 416 5.93e-14 70.5
MS.gene063201.t1 AT5G63370 35.714 126 71 4 18 139 297 416 5.93e-14 70.5
MS.gene063201.t1 AT5G63370 35.714 126 71 4 18 139 297 416 6.89e-14 70.5
MS.gene063201.t1 AT5G63370 35.714 126 71 4 18 139 297 416 6.89e-14 70.5
MS.gene063201.t1 AT5G63370 35.714 126 71 4 18 139 297 416 6.89e-14 70.5
MS.gene063201.t1 AT5G63370 35.714 126 71 4 18 139 297 416 6.89e-14 70.5
MS.gene063201.t1 AT5G63370 35.714 126 71 4 18 139 297 416 6.89e-14 70.5
MS.gene063201.t1 AT5G44290 30.052 193 88 5 17 169 136 321 7.28e-14 70.5
MS.gene063201.t1 AT5G44290 29.114 237 114 8 18 208 137 365 7.66e-14 70.1
MS.gene063201.t1 AT3G05050 37.410 139 75 5 5 140 124 253 9.24e-14 70.1
MS.gene063201.t1 AT3G05050 37.410 139 75 5 5 140 124 253 9.24e-14 70.1
MS.gene063201.t1 AT3G05050 37.410 139 75 5 5 140 124 253 9.24e-14 70.1
MS.gene063201.t1 AT5G50860 39.516 124 66 4 18 140 114 229 9.30e-14 70.1
MS.gene063201.t1 AT1G74330 39.844 128 64 4 16 140 119 236 1.25e-13 69.7
MS.gene063201.t1 AT1G74330 39.844 128 64 4 16 140 119 236 1.29e-13 69.7
MS.gene063201.t1 AT1G54610 37.984 129 65 4 16 140 116 233 1.31e-13 69.7
MS.gene063201.t1 AT1G54610 37.984 129 65 4 16 140 116 233 1.31e-13 69.7
MS.gene063201.t1 AT1G54610 37.984 129 65 4 16 140 116 233 1.31e-13 69.7
MS.gene063201.t1 AT5G44290 30.052 193 88 5 17 169 136 321 1.32e-13 69.7
MS.gene063201.t1 AT5G44290 30.052 193 88 5 17 169 136 321 1.32e-13 69.7
MS.gene063201.t1 AT5G44290 30.052 193 88 5 17 169 136 321 1.32e-13 69.7
MS.gene063201.t1 AT5G44290 30.052 193 88 5 17 169 136 321 1.32e-13 69.7
MS.gene063201.t1 AT5G44290 30.052 193 88 5 17 169 136 321 1.32e-13 69.7
MS.gene063201.t1 AT1G74330 39.844 128 64 4 16 140 119 236 1.46e-13 69.7
MS.gene063201.t1 AT5G50860 38.211 123 69 3 18 140 114 229 1.54e-13 69.3
MS.gene063201.t1 AT1G53050 43.478 92 48 2 16 107 132 219 4.96e-13 68.2
MS.gene063201.t1 AT1G53050 43.478 92 48 2 16 107 132 219 4.96e-13 68.2
MS.gene063201.t1 AT2G38620 75.610 41 10 0 16 56 2 42 3.45e-12 64.3
MS.gene063201.t1 AT3G01085 36.434 129 67 4 16 140 113 230 6.05e-12 64.7
MS.gene063201.t1 AT3G01085 36.434 129 67 4 16 140 113 230 6.05e-12 64.7
MS.gene063201.t1 AT3G01085 36.434 129 67 4 16 140 113 230 6.71e-12 64.7
MS.gene063201.t1 AT4G22940 34.307 137 83 4 5 140 89 219 1.73e-11 63.2
MS.gene063201.t1 AT4G22940 34.307 137 83 4 5 140 89 219 4.09e-11 62.4

Find 53 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCTGGAGTTTCAATAAT+TGG 0.211597 1.2:-26903203 MS.gene063201:CDS
AGGGAAAACTGTGTTGTATT+TGG 0.261009 1.2:-26904200 MS.gene063201:CDS
AACTTTGAATTTAACATTGC+TGG 0.301048 1.2:-26903234 MS.gene063201:CDS
AAGAAATTAACATACTTTGA+TGG 0.359794 1.2:+26904099 None:intergenic
TAACCTTTCTATTAATCATC+AGG 0.389375 1.2:+26903298 None:intergenic
ATTAACATACTTTGATGGTT+GGG 0.393821 1.2:+26904104 None:intergenic
CACTACTGCGATTGTTTCAC+CGG 0.398610 1.2:+26904550 None:intergenic
CTCATGATCCACATGTTGTT+AGG 0.404736 1.2:-26904343 MS.gene063201:intron
AACAATCGCAGTAGTGTTAT+CGG 0.409220 1.2:-26904543 MS.gene063201:CDS
TCTGTGGTTGCTTTCTGCCA+TGG 0.421970 1.2:-26903960 MS.gene063201:intron
AACCTCGACTTTGATATTCT+CGG 0.440454 1.2:-26903126 MS.gene063201:CDS
GAAACCTCGCGAAGCGTAGT+AGG 0.445811 1.2:+26904383 None:intergenic
AATTAACATACTTTGATGGT+TGG 0.452118 1.2:+26904103 None:intergenic
TTAACATACTTTGATGGTTG+GGG 0.473395 1.2:+26904105 None:intergenic
AGAGCAAGAGAGAAAGCTAC+TGG 0.475204 1.2:-26904461 MS.gene063201:CDS
ATACCTGATGATTAATAGAA+AGG 0.478991 1.2:-26903301 MS.gene063201:CDS
TGCAAGGGTGTTGCTTTCTG+TGG 0.485498 1.2:-26903976 MS.gene063201:CDS
ACCTCGACTTTGATATTCTC+GGG 0.485811 1.2:-26903125 MS.gene063201:CDS
TTCGAGAAGCTCGAGAAAGT+TGG 0.501777 1.2:-26904509 MS.gene063201:CDS
TCAATGTTGGTATCGACATA+AGG 0.502458 1.2:-26903257 MS.gene063201:CDS
TTTGATGTATCAACTTTGCA+AGG 0.507138 1.2:-26903992 MS.gene063201:CDS
CTGATCTTAAGAAATTCATT+CGG 0.515537 1.2:-26904159 MS.gene063201:CDS
AAAGTTGGAGGAGGAACGTA+CGG 0.523551 1.2:-26904494 MS.gene063201:CDS
CGCAGTAGTGTTATCGGCGA+AGG 0.529473 1.2:-26904537 MS.gene063201:CDS
TACTTCCGACGATGTGAAAG+TGG 0.553582 1.2:+26903169 None:intergenic
TCCTCCTACTACGCTTCGCG+AGG 0.556697 1.2:-26904387 MS.gene063201:CDS
GAGAAGCTCGAGAAAGTTGG+AGG 0.563232 1.2:-26904506 MS.gene063201:CDS
CGTCTTCATGAAGATGATGA+AGG 0.573237 1.2:-26904413 MS.gene063201:CDS
TTGAATTTAACATTGCTGGA+AGG 0.576174 1.2:-26903230 MS.gene063201:CDS
ACCTCGCGAAGCGTAGTAGG+AGG 0.577549 1.2:+26904386 None:intergenic
TCTGGAGTTTCAATAATTGG+AGG 0.578625 1.2:-26903200 MS.gene063201:CDS
AACAAGGACAAAACAAGGAA+GGG 0.584761 1.2:-26904219 MS.gene063201:CDS
AAACAAGGACAAAACAAGGA+AGG 0.590466 1.2:-26904220 MS.gene063201:CDS
ATAGAAAGGTTAACAAGTGG+TGG 0.599117 1.2:-26903287 MS.gene063201:CDS
TGTCGATACCAACATTGAAG+TGG 0.599252 1.2:+26903262 None:intergenic
AAGCTCGAGAAAGTTGGAGG+AGG 0.601692 1.2:-26904503 MS.gene063201:CDS
TTGATGTATCAACTTTGCAA+GGG 0.602717 1.2:-26903991 MS.gene063201:CDS
AACCTCCACTTTCACATCGT+CGG 0.603890 1.2:-26903174 MS.gene063201:CDS
TTAACTTACCTAACAACATG+TGG 0.608876 1.2:+26904335 None:intergenic
TAACATACTTTGATGGTTGG+GGG 0.617575 1.2:+26904106 None:intergenic
GAAGTATTCTCAATTACATG+TGG 0.624167 1.2:-26903152 MS.gene063201:CDS
GAGCAAGAGAGAAAGCTACT+GGG 0.629277 1.2:-26904460 MS.gene063201:CDS
AAGTTGGAGGAGGAACGTAC+GGG 0.630808 1.2:-26904493 MS.gene063201:CDS
TCCCGAGAATATCAAAGTCG+AGG 0.631210 1.2:+26903124 None:intergenic
GTATTCTCAATTACATGTGG+AGG 0.636280 1.2:-26903149 MS.gene063201:CDS
ACTACTGCGATTGTTTCACC+GGG 0.640436 1.2:+26904551 None:intergenic
TGGAGGAGGAACGTACGGGA+AGG 0.646655 1.2:-26904489 MS.gene063201:CDS
TTAATAGAAAGGTTAACAAG+TGG 0.651141 1.2:-26903290 MS.gene063201:CDS
GATTCAATCATGGAGAAACC+CGG 0.652544 1.2:-26904569 None:intergenic
TGTGAAACAAGGACAAAACA+AGG 0.655699 1.2:-26904224 MS.gene063201:CDS
TGGTGGTGCCACTTCAATGT+TGG 0.668205 1.2:-26903270 MS.gene063201:CDS
AGGTTGTTGGATGTGAAACA+AGG 0.680859 1.2:-26904235 MS.gene063201:CDS
TTCCGACGATGTGAAAGTGG+AGG 0.691070 1.2:+26903172 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTAAAAAAAAAAAAAT+TGG - chr1.2:26903891-26903910 MS.gene063201:intron 0.0%
!!! TTTTTTTTTTTTTAAAAAAA+AGG + chr1.2:26903890-26903909 None:intergenic 0.0%
!! AAATATTATTTATGGTTTAA+TGG - chr1.2:26903745-26903764 MS.gene063201:intron 10.0%
!! TTTAAAAAAAAAAAAATTGG+AGG - chr1.2:26903894-26903913 MS.gene063201:intron 10.0%
!!! TTTTGTATTTAAATTAGTTA+GGG - chr1.2:26904123-26904142 MS.gene063201:CDS 10.0%
!! AATTTCAATTCAATTTCAAT+TGG - chr1.2:26903367-26903386 MS.gene063201:intron 15.0%
!! ATGGAAGAAATTAAAAAAAA+AGG + chr1.2:26903934-26903953 None:intergenic 15.0%
!! ATTGAAATTGAAAACAATTT+AGG + chr1.2:26903356-26903375 None:intergenic 15.0%
!! TTGAAATTGAAAACAATTTA+GGG + chr1.2:26903355-26903374 None:intergenic 15.0%
!! TTTGTATTTAAATTAGTTAG+GGG - chr1.2:26904124-26904143 MS.gene063201:CDS 15.0%
!!! GTTTTGTATTTAAATTAGTT+AGG - chr1.2:26904122-26904141 MS.gene063201:CDS 15.0%
!!! TGGATTTGAAATATTATTTA+TGG - chr1.2:26903737-26903756 MS.gene063201:intron 15.0%
!!! TTTCAATTGGATTTAAAATT+AGG - chr1.2:26903380-26903399 MS.gene063201:intron 15.0%
!! AAGAAATTAACATACTTTGA+TGG + chr1.2:26903568-26903587 None:intergenic 20.0%
!! ATTGGAGGAAATTAAAAAAA+TGG + chr1.2:26903953-26903972 None:intergenic 20.0%
!! TTGTATTTAAATTAGTTAGG+GGG - chr1.2:26904125-26904144 MS.gene063201:CDS 20.0%
!!! ATTTTATGGGTTTGATTTTA+GGG - chr1.2:26903649-26903668 MS.gene063201:intron 20.0%
!!! TAGTTTTTTTTTATTCTTGC+AGG + chr1.2:26903985-26904004 None:intergenic 20.0%
!!! TGATCTCATTTATTTTCTTA+TGG - chr1.2:26904321-26904340 MS.gene063201:intron 20.0%
!!! TTTTCACATGCATATTTATT+AGG + chr1.2:26904199-26904218 None:intergenic 20.0%
! AACTTTGAATTTAACATTGC+TGG - chr1.2:26904430-26904449 MS.gene063201:CDS 25.0%
! AGCAATTATCAAAACAATCT+AGG + chr1.2:26904294-26904313 None:intergenic 25.0%
! ATACCTGATGATTAATAGAA+AGG - chr1.2:26904363-26904382 MS.gene063201:CDS 25.0%
! CTAAAAAAAAAATATCCACG+TGG - chr1.2:26904002-26904021 MS.gene063201:intron 25.0%
! CTGATCTTAAGAAATTCATT+CGG - chr1.2:26903505-26903524 MS.gene063201:intron 25.0%
! GCAATTATCAAAACAATCTA+GGG + chr1.2:26904293-26904312 None:intergenic 25.0%
! TAACCTTTCTATTAATCATC+AGG + chr1.2:26904369-26904388 None:intergenic 25.0%
! TTAATAGAAAGGTTAACAAG+TGG - chr1.2:26904374-26904393 MS.gene063201:CDS 25.0%
!! AATTAACATACTTTGATGGT+TGG + chr1.2:26903564-26903583 None:intergenic 25.0%
!! ATTAACATACTTTGATGGTT+GGG + chr1.2:26903563-26903582 None:intergenic 25.0%
!!! AAATCCATTTTTTCAAACCA+TGG + chr1.2:26903724-26903743 None:intergenic 25.0%
!!! AGATTGTTTTGATAATTGCT+AGG - chr1.2:26904294-26904313 MS.gene063201:intron 25.0%
!!! GATTTTATGGGTTTGATTTT+AGG - chr1.2:26903648-26903667 MS.gene063201:intron 25.0%
!!! GTATTTGGTTTTTGAGTATA+TGG - chr1.2:26903479-26903498 MS.gene063201:intron 25.0%
!!! GTTGATTTTGTGAATTTTGT+TGG - chr1.2:26903402-26903421 MS.gene063201:intron 25.0%
!!! TTCTGTTATTTCTGGAATTT+TGG - chr1.2:26903771-26903790 MS.gene063201:intron 25.0%
ATGAAATCTTCTCAATTGAG+TGG - chr1.2:26904262-26904281 MS.gene063201:intron 30.0%
TGCCAAAGATTCAATACTTA+GGG - chr1.2:26904095-26904114 MS.gene063201:intron 30.0%
TTAACTTACCTAACAACATG+TGG + chr1.2:26903332-26903351 None:intergenic 30.0%
TTGAATTTAACATTGCTGGA+AGG - chr1.2:26904434-26904453 MS.gene063201:CDS 30.0%
TTGATGTATCAACTTTGCAA+GGG - chr1.2:26903673-26903692 MS.gene063201:intron 30.0%
TTTGATGTATCAACTTTGCA+AGG - chr1.2:26903672-26903691 MS.gene063201:intron 30.0%
! AGACTAACACTTTTCTTTTG+CGG + chr1.2:26903867-26903886 None:intergenic 30.0%
! GAAGTATTCTCAATTACATG+TGG - chr1.2:26904512-26904531 MS.gene063201:CDS 30.0%
! TATTGAATCTTTGGCAGTTT+TGG + chr1.2:26904091-26904110 None:intergenic 30.0%
! TCATCTGGAGTTTCAATAAT+TGG - chr1.2:26904461-26904480 MS.gene063201:CDS 30.0%
! TCTTGCAGGCATTTTTAAAT+TGG + chr1.2:26903971-26903990 None:intergenic 30.0%
! TTAACATACTTTGATGGTTG+GGG + chr1.2:26903562-26903581 None:intergenic 30.0%
! TTGTGTTGTTCTTGGTAAAA+TGG - chr1.2:26903815-26903834 MS.gene063201:intron 30.0%
!! AATGGTGATTCTGTTATTTC+TGG - chr1.2:26903763-26903782 MS.gene063201:intron 30.0%
!! TTGTGAATTTTGTTGGTGTT+AGG - chr1.2:26903409-26903428 MS.gene063201:intron 30.0%
AAACAAGGACAAAACAAGGA+AGG - chr1.2:26903444-26903463 MS.gene063201:intron 35.0%
AACAAGGACAAAACAAGGAA+GGG - chr1.2:26903445-26903464 MS.gene063201:intron 35.0%
AACAATCGCAGTAGTGTTAT+CGG - chr1.2:26903121-26903140 MS.gene063201:CDS 35.0%
AACCTCGACTTTGATATTCT+CGG - chr1.2:26904538-26904557 MS.gene063201:CDS 35.0%
AGGGAAAACTGTGTTGTATT+TGG - chr1.2:26903464-26903483 MS.gene063201:intron 35.0%
ATAGAAAGGTTAACAAGTGG+TGG - chr1.2:26904377-26904396 MS.gene063201:CDS 35.0%
ATCAACGACCAAGTCAAAAA+TGG - chr1.2:26904056-26904075 MS.gene063201:intron 35.0%
ATTAGGCTTAATTGCACGTT+TGG + chr1.2:26904182-26904201 None:intergenic 35.0%
CCAAAGATTCAATACTTAGG+GGG - chr1.2:26904097-26904116 MS.gene063201:intron 35.0%
CTGCCAAAGATTCAATACTT+AGG - chr1.2:26904094-26904113 MS.gene063201:intron 35.0%
GCCAAAGATTCAATACTTAG+GGG - chr1.2:26904096-26904115 MS.gene063201:intron 35.0%
GTATTCTCAATTACATGTGG+AGG - chr1.2:26904515-26904534 MS.gene063201:CDS 35.0%
TCTGCCATGGTTTGAAAAAA+TGG - chr1.2:26903717-26903736 MS.gene063201:intron 35.0%
TGTGAAACAAGGACAAAACA+AGG - chr1.2:26903440-26903459 MS.gene063201:intron 35.0%
TTGGTTCATAGTGTTCTTCA+AGG - chr1.2:26903790-26903809 MS.gene063201:intron 35.0%
! TAACATACTTTGATGGTTGG+GGG + chr1.2:26903561-26903580 None:intergenic 35.0%
! TCTGGAGTTTCAATAATTGG+AGG - chr1.2:26904464-26904483 MS.gene063201:CDS 35.0%
! TGCAGGCATTTTTAAATTGG+AGG + chr1.2:26903968-26903987 None:intergenic 35.0%
!! TCAAGGTTTTGTGTTGTTCT+TGG - chr1.2:26903807-26903826 MS.gene063201:intron 35.0%
!! TCAATGTTGGTATCGACATA+AGG - chr1.2:26904407-26904426 MS.gene063201:CDS 35.0%
!!! TTTTGTTGGTGTTAGGTTGT+TGG - chr1.2:26903416-26903435 MS.gene063201:intron 35.0%
ACCAAGTCAAAAATGGACCA+GGG - chr1.2:26904063-26904082 MS.gene063201:intron 40.0%
ACCTCGACTTTGATATTCTC+GGG - chr1.2:26904539-26904558 MS.gene063201:CDS 40.0%
AGGTTGTTGGATGTGAAACA+AGG - chr1.2:26903429-26903448 MS.gene063201:intron 40.0%
CCCTAACTTTCCAAAAGTTG+CGG + chr1.2:26904157-26904176 None:intergenic 40.0%
CGTCTTCATGAAGATGATGA+AGG - chr1.2:26903251-26903270 MS.gene063201:CDS 40.0%
CTCATGATCCACATGTTGTT+AGG - chr1.2:26903321-26903340 MS.gene063201:intron 40.0%
CTTTCCAAAAGTTGCGGTTA+TGG + chr1.2:26904151-26904170 None:intergenic 40.0%
TGTCGATACCAACATTGAAG+TGG + chr1.2:26904405-26904424 None:intergenic 40.0%
! ATTCGGAGTTTTCGTCAAAC+TGG - chr1.2:26903522-26903541 MS.gene063201:intron 40.0%
! TTCGGAGTTTTCGTCAAACT+GGG - chr1.2:26903523-26903542 MS.gene063201:intron 40.0%
! TTGCTGGAAGGATTTTCATC+TGG - chr1.2:26904446-26904465 MS.gene063201:CDS 40.0%
!! ACCGCAACTTTTGGAAAGTT+AGG - chr1.2:26904153-26904172 MS.gene063201:CDS 40.0%
!! CCCCCTAAGTATTGAATCTT+TGG + chr1.2:26904100-26904119 None:intergenic 40.0%
!! CCGCAACTTTTGGAAAGTTA+GGG - chr1.2:26904154-26904173 MS.gene063201:CDS 40.0%
!! CGCAACTTTTGGAAAGTTAG+GGG - chr1.2:26904155-26904174 MS.gene063201:CDS 40.0%
!! GCAACTTTTGGAAAGTTAGG+GGG - chr1.2:26904156-26904175 MS.gene063201:CDS 40.0%
AAAGTTGGAGGAGGAACGTA+CGG - chr1.2:26903170-26903189 MS.gene063201:CDS 45.0%
AACCTCCACTTTCACATCGT+CGG - chr1.2:26904490-26904509 MS.gene063201:CDS 45.0%
ACTACTGCGATTGTTTCACC+GGG + chr1.2:26903116-26903135 None:intergenic 45.0%
AGAGCAAGAGAGAAAGCTAC+TGG - chr1.2:26903203-26903222 MS.gene063201:CDS 45.0%
CAAGTCAAAAATGGACCAGG+GGG - chr1.2:26904065-26904084 MS.gene063201:intron 45.0%
CACTACTGCGATTGTTTCAC+CGG + chr1.2:26903117-26903136 None:intergenic 45.0%
CCAAGTCAAAAATGGACCAG+GGG - chr1.2:26904064-26904083 MS.gene063201:intron 45.0%
GACCAAGTCAAAAATGGACC+AGG - chr1.2:26904062-26904081 MS.gene063201:intron 45.0%
GAGCAAGAGAGAAAGCTACT+GGG - chr1.2:26903204-26903223 MS.gene063201:CDS 45.0%
TACTTCCGACGATGTGAAAG+TGG + chr1.2:26904498-26904517 None:intergenic 45.0%
TCCCGAGAATATCAAAGTCG+AGG + chr1.2:26904543-26904562 None:intergenic 45.0%
TTCGAGAAGCTCGAGAAAGT+TGG - chr1.2:26903155-26903174 MS.gene063201:CDS 45.0%
!!! TAATTAATTAATGATTTTAT+GGG - chr1.2:26903636-26903655 MS.gene063201:intron 5.0%
!!! TTAATTAATTAATGATTTTA+TGG - chr1.2:26903635-26903654 MS.gene063201:intron 5.0%
AAGCTCGAGAAAGTTGGAGG+AGG - chr1.2:26903161-26903180 MS.gene063201:CDS 50.0%
AAGTTGGAGGAGGAACGTAC+GGG - chr1.2:26903171-26903190 MS.gene063201:CDS 50.0%
CACGTGGCACCTAACTTAAG+GGG - chr1.2:26904018-26904037 MS.gene063201:intron 50.0%
CCACGTGGCACCTAACTTAA+GGG - chr1.2:26904017-26904036 MS.gene063201:intron 50.0%
GAGAAGCTCGAGAAAGTTGG+AGG - chr1.2:26903158-26903177 MS.gene063201:CDS 50.0%
TCCACGTGGCACCTAACTTA+AGG - chr1.2:26904016-26904035 MS.gene063201:intron 50.0%
TTCCGACGATGTGAAAGTGG+AGG + chr1.2:26904495-26904514 None:intergenic 50.0%
! CATACTTTGATGGTTGGGGG+TGG + chr1.2:26903558-26903577 None:intergenic 50.0%
! GCACCTAACTTAAGGGGTGT+GGG - chr1.2:26904024-26904043 MS.gene063201:intron 50.0%
! TCTGTGGTTGCTTTCTGCCA+TGG - chr1.2:26903704-26903723 MS.gene063201:intron 50.0%
!! CCCCTGGTCCATTTTTGACT+TGG + chr1.2:26904067-26904086 None:intergenic 50.0%
!! TGACTTGGTCGTTGATGACG+TGG + chr1.2:26904052-26904071 None:intergenic 50.0%
!! TGCAAGGGTGTTGCTTTCTG+TGG - chr1.2:26903688-26903707 MS.gene063201:intron 50.0%
!! TGGTGGTGCCACTTCAATGT+TGG - chr1.2:26904394-26904413 MS.gene063201:CDS 50.0%
CGCAGTAGTGTTATCGGCGA+AGG - chr1.2:26903127-26903146 MS.gene063201:CDS 55.0%
GAAACCTCGCGAAGCGTAGT+AGG + chr1.2:26903284-26903303 None:intergenic 55.0%
GCACCCACACCCCTTAAGTT+AGG + chr1.2:26904030-26904049 None:intergenic 55.0%
GGCACCTAACTTAAGGGGTG+TGG - chr1.2:26904023-26904042 MS.gene063201:intron 55.0%
! CCCTTAAGTTAGGTGCCACG+TGG + chr1.2:26904020-26904039 None:intergenic 55.0%
! GGGGCCATAACCGCAACTTT+TGG - chr1.2:26904144-26904163 MS.gene063201:CDS 55.0%
ACCTCGCGAAGCGTAGTAGG+AGG + chr1.2:26903281-26903300 None:intergenic 60.0%
TCCTCCTACTACGCTTCGCG+AGG - chr1.2:26903277-26903296 MS.gene063201:CDS 60.0%
TGGAGGAGGAACGTACGGGA+AGG - chr1.2:26903175-26903194 MS.gene063201:CDS 60.0%
!! CTTTGGCAGTTTTGGCCCCC+TGG + chr1.2:26904083-26904102 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 26903104 26904582 26903104 ID=MS.gene063201
chr1.2 mRNA 26903104 26904582 26903104 ID=MS.gene063201.t1;Parent=MS.gene063201
chr1.2 exon 26904344 26904582 26904344 ID=MS.gene063201.t1.exon1;Parent=MS.gene063201.t1
chr1.2 CDS 26904344 26904582 26904344 ID=cds.MS.gene063201.t1;Parent=MS.gene063201.t1
chr1.2 exon 26904114 26904255 26904114 ID=MS.gene063201.t1.exon2;Parent=MS.gene063201.t1
chr1.2 CDS 26904114 26904255 26904114 ID=cds.MS.gene063201.t1;Parent=MS.gene063201.t1
chr1.2 exon 26903961 26904016 26903961 ID=MS.gene063201.t1.exon3;Parent=MS.gene063201.t1
chr1.2 CDS 26903961 26904016 26903961 ID=cds.MS.gene063201.t1;Parent=MS.gene063201.t1
chr1.2 exon 26903104 26903329 26903104 ID=MS.gene063201.t1.exon4;Parent=MS.gene063201.t1
chr1.2 CDS 26903104 26903329 26903104 ID=cds.MS.gene063201.t1;Parent=MS.gene063201.t1
Gene Sequence

>MS.gene063201

ATGGAGAAACCCGGTGAAACAATCGCAGTAGTGTTATCGGCGAAGGAAGCATTCGAGAAGCTCGAGAAAGTTGGAGGAGGAACGTACGGGAAGGTTTACAGAGCAAGAGAGAAAGCTACTGGGAAGATTGTTGCGTTGAAGAAGACTCGTCTTCATGAAGATGATGAAGGTGTTCCTCCTACTACGCTTCGCGAGGTTTCGATTTTGTGTATGTTGTCTCATGATCCACATGTTGTTAGGTAAGTTAACCCTAAATTGTTTTCAATTTCAATTCAATTTCAATTGGATTTAAAATTAGGTTGATTTTGTGAATTTTGTTGGTGTTAGGTTGTTGGATGTGAAACAAGGACAAAACAAGGAAGGGAAAACTGTGTTGTATTTGGTTTTTGAGTATATGGATACTGATCTTAAGAAATTCATTCGGAGTTTTCGTCAAACTGGGCAAAATATTCCACCCCCAACCATCAAAGTATGTTAATTTCTTTTCAGATCTTCAAATTATTTGATTATTGTTGTTAGTTTTTTTTGCAATTAATTAATTAATGATTTTATGGGTTTGATTTTAGGGTTTGATGTATCAACTTTGCAAGGGTGTTGCTTTCTGTGGTTGCTTTCTGCCATGGTTTGAAAAAATGGATTTGAAATATTATTTATGGTTTAATGGTGATTCTGTTATTTCTGGAATTTTGGTTCATAGTGTTCTTCAAGGTTTTGTGTTGTTCTTGGTAAAATGGAGTTTTGATCTATGATTTTGTAGCTGCCGCAAAAGAAAAGTGTTAGTCTCCTTTTTTTTAAAAAAAAAAAAATTGGAGGTCTATTTTCTTTTTCCTTTTTTTTTAATTTCTTCCATTTTTTTAATTTCCTCCAATTTAAAAATGCCTGCAAGAATAAAAAAAAACTAAAAAAAAAATATCCACGTGGCACCTAACTTAAGGGGTGTGGGTGCCACGTCATCAACGACCAAGTCAAAAATGGACCAGGGGGCCAAAACTGCCAAAGATTCAATACTTAGGGGGCTGTTTTGTATTTAAATTAGTTAGGGGGCCATAACCGCAACTTTTGGAAAGTTAGGGGGCCAAACGTGCAATTAAGCCTAATAAATATGCATGTGAAAATTTTGTAATAAAAAATTAGCATCAATTTAATAGTTTGCATCTTATGAAATCTTCTCAATTGAGTGGATAGTCCCTAGATTGTTTTGATAATTGCTAGGTTGTTGATCTCATTTATTTTCTTATGGAATAATCTTGTAGTAAATCATACCTGATGATTAATAGAAAGGTTAACAAGTGGTGGTGCCACTTCAATGTTGGTATCGACATAAGGAACTTTGAATTTAACATTGCTGGAAGGATTTTCATCTGGAGTTTCAATAATTGGAGGTGTAACCTCCACTTTCACATCGTCGGAAGTATTCTCAATTACATGTGGAGGAACCTCGACTTTGATATTCTCGGGATAATTCAAAATTTGAAGTGA
Protein sequence

>MS.gene063201.t1

MEKPGETIAVVLSAKEAFEKLEKVGGGTYGKVYRAREKATGKIVALKKTRLHEDDEGVPPTTLREVSILCMLSHDPHVVRLLDVKQGQNKEGKTVLYLVFEYMDTDLKKFIRSFRQTGQNIPPPTIKGLMYQLCKGVAFCGCFLPCKSYLMINRKVNKWWCHFNVGIDIRNFEFNIAGRIFIWSFNNWRCNLHFHIVGSILNYMWRNLDFDILGIIQNLK