Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063229.t1 | XP_013467071.1 | 86.4 | 198 | 27 | 0 | 1 | 198 | 1 | 198 | 1.90E-52 | 215.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063229.t1 | Q9FJW5 | 77.2 | 57 | 13 | 0 | 1 | 57 | 1 | 57 | 2.2e-20 | 100.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063229.t1 | A0A072VHK1 | 86.4 | 198 | 27 | 0 | 1 | 198 | 1 | 198 | 1.4e-52 | 215.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene063229.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063229.t1 | MTR_1g041765 | 88.384 | 198 | 23 | 0 | 1 | 198 | 1 | 198 | 5.41e-110 | 313 |
| MS.gene063229.t1 | MTR_5g015340 | 67.416 | 89 | 27 | 1 | 1 | 87 | 1 | 89 | 4.34e-35 | 125 |
| MS.gene063229.t1 | MTR_5g015340 | 67.416 | 89 | 27 | 1 | 1 | 87 | 1 | 89 | 4.34e-35 | 125 |
| MS.gene063229.t1 | MTR_5g015340 | 67.416 | 89 | 27 | 1 | 1 | 87 | 1 | 89 | 5.30e-34 | 124 |
| MS.gene063229.t1 | MTR_4g097130 | 66.292 | 89 | 29 | 1 | 1 | 88 | 1 | 89 | 8.49e-34 | 122 |
| MS.gene063229.t1 | MTR_4g097130 | 66.292 | 89 | 29 | 1 | 1 | 88 | 1 | 89 | 9.74e-34 | 122 |
| MS.gene063229.t1 | MTR_4g097130 | 66.292 | 89 | 29 | 1 | 1 | 88 | 1 | 89 | 1.01e-33 | 122 |
| MS.gene063229.t1 | MTR_0095s0040 | 57.447 | 94 | 36 | 2 | 1 | 90 | 1 | 94 | 8.77e-28 | 105 |
| MS.gene063229.t1 | MTR_8g494240 | 57.447 | 94 | 36 | 2 | 1 | 90 | 1 | 94 | 1.96e-27 | 105 |
| MS.gene063229.t1 | MTR_8g494240 | 57.447 | 94 | 36 | 2 | 1 | 90 | 1 | 94 | 2.24e-27 | 105 |
| MS.gene063229.t1 | MTR_8g494240 | 57.447 | 94 | 36 | 2 | 1 | 90 | 1 | 94 | 8.40e-27 | 105 |
| MS.gene063229.t1 | MTR_2g031870 | 72.131 | 61 | 17 | 0 | 1 | 61 | 1 | 61 | 9.58e-27 | 103 |
| MS.gene063229.t1 | MTR_8g092810 | 71.930 | 57 | 16 | 0 | 1 | 57 | 1 | 57 | 7.61e-24 | 98.2 |
| MS.gene063229.t1 | MTR_2g099610 | 67.857 | 56 | 18 | 0 | 1 | 56 | 1 | 56 | 1.48e-20 | 87.4 |
| MS.gene063229.t1 | MTR_8g018410 | 41.584 | 101 | 52 | 1 | 1 | 101 | 1 | 94 | 4.35e-18 | 80.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063229.t1 | AT5G67580 | 64.706 | 85 | 28 | 1 | 1 | 83 | 1 | 85 | 1.92e-30 | 113 |
| MS.gene063229.t1 | AT5G67580 | 64.706 | 85 | 28 | 1 | 1 | 83 | 1 | 85 | 1.92e-30 | 113 |
| MS.gene063229.t1 | AT3G49850 | 54.348 | 92 | 42 | 0 | 1 | 92 | 1 | 92 | 2.03e-27 | 105 |
| MS.gene063229.t1 | AT3G49850 | 54.348 | 92 | 42 | 0 | 1 | 92 | 1 | 92 | 2.03e-27 | 105 |
| MS.gene063229.t1 | AT1G49950 | 74.138 | 58 | 15 | 0 | 1 | 58 | 1 | 58 | 4.62e-26 | 102 |
| MS.gene063229.t1 | AT1G49950 | 74.138 | 58 | 15 | 0 | 1 | 58 | 1 | 58 | 4.62e-26 | 102 |
| MS.gene063229.t1 | AT1G49950 | 74.138 | 58 | 15 | 0 | 1 | 58 | 1 | 58 | 4.62e-26 | 102 |
| MS.gene063229.t1 | AT1G72740 | 60.938 | 64 | 25 | 0 | 1 | 64 | 1 | 64 | 2.48e-20 | 86.3 |
| MS.gene063229.t1 | AT1G72740 | 60.938 | 64 | 25 | 0 | 1 | 64 | 1 | 64 | 2.59e-20 | 86.3 |
| MS.gene063229.t1 | AT1G17520 | 59.091 | 66 | 27 | 0 | 1 | 66 | 1 | 66 | 4.49e-20 | 85.9 |
| MS.gene063229.t1 | AT1G17520 | 59.091 | 66 | 27 | 0 | 1 | 66 | 1 | 66 | 4.62e-20 | 85.9 |
| MS.gene063229.t1 | AT1G17520 | 59.091 | 66 | 27 | 0 | 1 | 66 | 1 | 66 | 4.75e-20 | 85.9 |
| MS.gene063229.t1 | AT1G17520 | 59.091 | 66 | 27 | 0 | 1 | 66 | 1 | 66 | 4.76e-20 | 85.9 |
Find 29 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCAAAATGATATTGAATTC+TGG | 0.176544 | 1.3:+25042372 | None:intergenic |
| TTATTTCTCTCGGTTGCTTT+TGG | 0.246977 | 1.3:+25042141 | None:intergenic |
| GCAAAATGATATTGAATTCT+GGG | 0.359677 | 1.3:+25042373 | None:intergenic |
| CAGAGCCGCCTCTTCTTCTC+CGG | 0.417460 | 1.3:+25042449 | None:intergenic |
| GAATATCAATATTCGATCAT+AGG | 0.430998 | 1.3:-25042212 | MS.gene063229:CDS |
| GAGGGGTCTAACACCTCAAC+AGG | 0.435246 | 1.3:-25042061 | MS.gene063229:CDS |
| ATGTAGACCTCAAGGACAAA+TGG | 0.452440 | 1.3:-25042338 | MS.gene063229:CDS |
| GTTGACATCATTATTTCTCT+CGG | 0.490773 | 1.3:+25042131 | None:intergenic |
| GGCAGAAGCTGCCATGACTA+AGG | 0.496803 | 1.3:-25042007 | MS.gene063229:CDS |
| GAAGAGGCGGCTCTGAAAGC+GGG | 0.499745 | 1.3:-25042441 | MS.gene063229:CDS |
| GGAGTAGTCAAACACGGAGC+TGG | 0.501232 | 1.3:-25042420 | MS.gene063229:CDS |
| AGTTCCTCCATTTGTCCTTG+AGG | 0.505844 | 1.3:+25042331 | None:intergenic |
| TCAAACACGGAGCTGGAAGA+TGG | 0.524401 | 1.3:-25042413 | MS.gene063229:CDS |
| CGACGACAACATTTAGATCA+AGG | 0.524618 | 1.3:-25042302 | MS.gene063229:CDS |
| TGCTTCCTCAGCCTTAGTCA+TGG | 0.542408 | 1.3:+25041996 | None:intergenic |
| AATAATAATCAATATTCAAG+TGG | 0.553519 | 1.3:-25042234 | MS.gene063229:CDS |
| AGCTGCAAAAGCAATGGAGA+AGG | 0.564388 | 1.3:-25042028 | MS.gene063229:CDS |
| AGCTGCCATGACTAAGGCTG+AGG | 0.573794 | 1.3:-25042001 | MS.gene063229:CDS |
| AAAATGGACCGGAGAAGAAG+AGG | 0.581842 | 1.3:-25042457 | MS.gene063229:CDS |
| AGAAGAAGCTGCAAAAGCAA+TGG | 0.585302 | 1.3:-25042034 | MS.gene063229:CDS |
| TGCAAAAGCAGAAATGAAGA+AGG | 0.586897 | 1.3:-25041923 | MS.gene063229:CDS |
| GCTTCTTCTATCGCCTGTTG+AGG | 0.590627 | 1.3:+25042048 | None:intergenic |
| GGAAGCAACTAATGAGGCAG+AGG | 0.590847 | 1.3:-25041980 | MS.gene063229:CDS |
| TCGCTCAAATGTAGACCTCA+AGG | 0.605114 | 1.3:-25042346 | MS.gene063229:CDS |
| GGCTGAGGAAGCAACTAATG+AGG | 0.610331 | 1.3:-25041986 | MS.gene063229:CDS |
| AGAAGAGGCGGCTCTGAAAG+CGG | 0.614833 | 1.3:-25042442 | MS.gene063229:CDS |
| TAGACCTCAAGGACAAATGG+AGG | 0.657376 | 1.3:-25042335 | MS.gene063229:CDS |
| ATGGACCGGAGAAGAAGAGG+CGG | 0.688711 | 1.3:-25042454 | MS.gene063229:CDS |
| AAAGCGGGAGTAGTCAAACA+CGG | 0.721145 | 1.3:-25042426 | MS.gene063229:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATAATCAATATTCAAG+TGG | - | chr1.3:25042140-25042159 | MS.gene063229:CDS | 15.0% |
| !!! | TGATTATTATTTTCAACTTT+AGG | + | chr1.3:25042131-25042150 | None:intergenic | 15.0% |
| !!! | AAGAGATTTTTAAAATGATG+AGG | - | chr1.3:25042294-25042313 | MS.gene063229:CDS | 20.0% |
| !!! | AGAGATTTTTAAAATGATGA+GGG | - | chr1.3:25042295-25042314 | MS.gene063229:CDS | 20.0% |
| !!! | TAATTTCTTCGTATTTTGAA+AGG | + | chr1.3:25042271-25042290 | None:intergenic | 20.0% |
| !!! | TTATTATTTTCAACTTTAGG+TGG | + | chr1.3:25042128-25042147 | None:intergenic | 20.0% |
| ! | GAATATCAATATTCGATCAT+AGG | - | chr1.3:25042162-25042181 | MS.gene063229:CDS | 25.0% |
| ! | GCAAAATGATATTGAATTCT+GGG | + | chr1.3:25042004-25042023 | None:intergenic | 25.0% |
| !!! | GAGATTTTTAAAATGATGAG+GGG | - | chr1.3:25042296-25042315 | MS.gene063229:CDS | 25.0% |
| CGCAAAATGATATTGAATTC+TGG | + | chr1.3:25042005-25042024 | None:intergenic | 30.0% | |
| ! | GTTGACATCATTATTTCTCT+CGG | + | chr1.3:25042246-25042265 | None:intergenic | 30.0% |
| ! | TTATTTTCAACTTTAGGTGG+AGG | + | chr1.3:25042125-25042144 | None:intergenic | 30.0% |
| AACTTCAACAAAAATACGCG+AGG | - | chr1.3:25042193-25042212 | MS.gene063229:CDS | 35.0% | |
| TGCAAAAGCAGAAATGAAGA+AGG | - | chr1.3:25042451-25042470 | MS.gene063229:CDS | 35.0% | |
| !! | GCGTATTTTTGTTGAAGTTG+AGG | + | chr1.3:25042192-25042211 | None:intergenic | 35.0% |
| !! | TTATTTCTCTCGGTTGCTTT+TGG | + | chr1.3:25042236-25042255 | None:intergenic | 35.0% |
| AGAAGAAGCTGCAAAAGCAA+TGG | - | chr1.3:25042340-25042359 | MS.gene063229:CDS | 40.0% | |
| ATGTAGACCTCAAGGACAAA+TGG | - | chr1.3:25042036-25042055 | MS.gene063229:CDS | 40.0% | |
| CGACGACAACATTTAGATCA+AGG | - | chr1.3:25042072-25042091 | MS.gene063229:CDS | 40.0% | |
| AAAATGGACCGGAGAAGAAG+AGG | - | chr1.3:25041917-25041936 | MS.gene063229:CDS | 45.0% | |
| AAAGCGGGAGTAGTCAAACA+CGG | - | chr1.3:25041948-25041967 | MS.gene063229:CDS | 45.0% | |
| AGCTGCAAAAGCAATGGAGA+AGG | - | chr1.3:25042346-25042365 | MS.gene063229:CDS | 45.0% | |
| AGTTCCTCCATTTGTCCTTG+AGG | + | chr1.3:25042046-25042065 | None:intergenic | 45.0% | |
| GCTCCTAAGCAAAAATGGAC+CGG | - | chr1.3:25041906-25041925 | MS.gene063229:CDS | 45.0% | |
| TAGACCTCAAGGACAAATGG+AGG | - | chr1.3:25042039-25042058 | MS.gene063229:CDS | 45.0% | |
| TCGCTCAAATGTAGACCTCA+AGG | - | chr1.3:25042028-25042047 | MS.gene063229:CDS | 45.0% | |
| TGGGTGCTCCTAAGCAAAAA+TGG | - | chr1.3:25041901-25041920 | MS.gene063229:CDS | 45.0% | |
| !! | TCTCCGGTCCATTTTTGCTT+AGG | + | chr1.3:25041912-25041931 | None:intergenic | 45.0% |
| GCTTCTTCTATCGCCTGTTG+AGG | + | chr1.3:25042329-25042348 | None:intergenic | 50.0% | |
| GGAAGCAACTAATGAGGCAG+AGG | - | chr1.3:25042394-25042413 | MS.gene063229:CDS | 50.0% | |
| GGCTGAGGAAGCAACTAATG+AGG | - | chr1.3:25042388-25042407 | MS.gene063229:CDS | 50.0% | |
| TCAAACACGGAGCTGGAAGA+TGG | - | chr1.3:25041961-25041980 | MS.gene063229:CDS | 50.0% | |
| TGCTTCCTCAGCCTTAGTCA+TGG | + | chr1.3:25042381-25042400 | None:intergenic | 50.0% | |
| AGCTGCCATGACTAAGGCTG+AGG | - | chr1.3:25042373-25042392 | MS.gene063229:CDS | 55.0% | |
| ATGGACCGGAGAAGAAGAGG+CGG | - | chr1.3:25041920-25041939 | MS.gene063229:CDS | 55.0% | |
| GAGGGGTCTAACACCTCAAC+AGG | - | chr1.3:25042313-25042332 | MS.gene063229:CDS | 55.0% | |
| GGAGTAGTCAAACACGGAGC+TGG | - | chr1.3:25041954-25041973 | MS.gene063229:CDS | 55.0% | |
| GGCAGAAGCTGCCATGACTA+AGG | - | chr1.3:25042367-25042386 | MS.gene063229:CDS | 55.0% | |
| !! | AGAAGAGGCGGCTCTGAAAG+CGG | - | chr1.3:25041932-25041951 | MS.gene063229:CDS | 55.0% |
| CAGAGCCGCCTCTTCTTCTC+CGG | + | chr1.3:25041928-25041947 | None:intergenic | 60.0% | |
| !! | GAAGAGGCGGCTCTGAAAGC+GGG | - | chr1.3:25041933-25041952 | MS.gene063229:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 25041900 | 25042496 | 25041900 | ID=MS.gene063229 |
| chr1.3 | mRNA | 25041900 | 25042496 | 25041900 | ID=MS.gene063229.t1;Parent=MS.gene063229 |
| chr1.3 | exon | 25041900 | 25042496 | 25041900 | ID=MS.gene063229.t1.exon1;Parent=MS.gene063229.t1 |
| chr1.3 | CDS | 25041900 | 25042496 | 25041900 | ID=cds.MS.gene063229.t1;Parent=MS.gene063229.t1 |
| Gene Sequence |
| Protein sequence |
