Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063249.t1 | KEH41073.1 | 92.8 | 167 | 10 | 1 | 1 | 165 | 1 | 167 | 1.60E-84 | 322 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063249.t1 | A0A072VI02 | 92.8 | 167 | 10 | 1 | 1 | 165 | 1 | 167 | 1.2e-84 | 322.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene063249.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063249.t1 | MTR_1g041455 | 92.814 | 167 | 10 | 1 | 1 | 165 | 1 | 167 | 3.94e-114 | 320 |
| MS.gene063249.t1 | MTR_1g048880 | 60.606 | 165 | 53 | 3 | 1 | 165 | 1 | 153 | 8.76e-59 | 180 |
| MS.gene063249.t1 | MTR_5g056450 | 57.714 | 175 | 47 | 5 | 1 | 165 | 1 | 158 | 7.91e-52 | 162 |
| MS.gene063249.t1 | MTR_1g075780 | 36.709 | 158 | 81 | 7 | 11 | 165 | 4 | 145 | 4.45e-15 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTGGTAAACATGTGAATT+TGG | 0.166169 | 1.3:-24804747 | None:intergenic |
| GAAAATTGATTTCATTGATT+TGG | 0.219457 | 1.3:+24804842 | MS.gene063249:CDS |
| TAGAACCAACATTTAGAAAA+TGG | 0.241260 | 1.3:-24804701 | None:intergenic |
| TTTAGAAAATGGTTTCTTCT+TGG | 0.277705 | 1.3:-24804690 | None:intergenic |
| AGTGCTAAATCCAATGTTTG+AGG | 0.428346 | 1.3:-24804891 | None:intergenic |
| AAATGGTTTCTTCTTGGTTG+TGG | 0.446475 | 1.3:-24804684 | None:intergenic |
| CAACTCGGGTCCTCAAACAT+TGG | 0.448467 | 1.3:+24804881 | MS.gene063249:CDS |
| ATTGATTTCATTGATTTGGT+TGG | 0.453682 | 1.3:+24804846 | MS.gene063249:CDS |
| CCTTCTCATTGTATCCCACC+AGG | 0.456761 | 1.3:+24804609 | MS.gene063249:CDS |
| GAGAAGGAAATTGCACTTGA+GGG | 0.480683 | 1.3:-24804593 | None:intergenic |
| CTTCTTGTAGATGCTTCCAT+TGG | 0.494962 | 1.3:-24804764 | None:intergenic |
| CAAGAAGAATCTTCAAATGA+TGG | 0.496730 | 1.3:+24804780 | MS.gene063249:CDS |
| ATCTTCAAATGATGGTACTA+AGG | 0.499653 | 1.3:+24804788 | MS.gene063249:CDS |
| GGTTTCTTCTTGGTTGTGGT+TGG | 0.536103 | 1.3:-24804680 | None:intergenic |
| AGTCTCACATGGAGCACGAA+GGG | 0.538263 | 1.3:+24804532 | MS.gene063249:CDS |
| TGAGAAGGAAATTGCACTTG+AGG | 0.543034 | 1.3:-24804594 | None:intergenic |
| CCTCGTGTTACTAAAGCCAT+TGG | 0.551040 | 1.3:-24804430 | None:intergenic |
| GTTACTAAAGCCATTGGTCA+TGG | 0.561887 | 1.3:-24804424 | None:intergenic |
| CAAATTCACATGTTTACCAA+TGG | 0.564592 | 1.3:+24804748 | MS.gene063249:CDS |
| TTTACCACCGCCATGACCAA+TGG | 0.567001 | 1.3:+24804414 | None:intergenic |
| CTTGAGTGCTTATGAAAACT+CGG | 0.574814 | 1.3:-24804491 | None:intergenic |
| AACTCACATCGAGTCTCACA+TGG | 0.586787 | 1.3:+24804521 | MS.gene063249:CDS |
| CTCAAAACATTAATGATGCT+AGG | 0.612047 | 1.3:+24804652 | MS.gene063249:CDS |
| TCTATGCAACTTACACCACA+TGG | 0.620484 | 1.3:+24804720 | MS.gene063249:CDS |
| CCTGGTGGGATACAATGAGA+AGG | 0.625137 | 1.3:-24804609 | None:intergenic |
| TCAATTGGAACATGAAAACC+TGG | 0.634016 | 1.3:-24804627 | None:intergenic |
| GGTAATGATGATGACAACTC+GGG | 0.634854 | 1.3:+24804867 | MS.gene063249:CDS |
| TTGGAACATGAAAACCTGGT+GGG | 0.643254 | 1.3:-24804623 | None:intergenic |
| TGGTAATGATGATGACAACT+CGG | 0.654905 | 1.3:+24804866 | MS.gene063249:CDS |
| GTCTCACATGGAGCACGAAG+GGG | 0.660070 | 1.3:+24804533 | MS.gene063249:CDS |
| GAGTCTCACATGGAGCACGA+AGG | 0.667512 | 1.3:+24804531 | MS.gene063249:CDS |
| GAGGCATGATGTGTTAAACT+CGG | 0.676388 | 1.3:+24804449 | MS.gene063249:CDS |
| GATAAGAAGACTCTATTCGA+CGG | 0.678238 | 1.3:+24804560 | MS.gene063249:CDS |
| CCAATGGCTTTAGTAACACG+AGG | 0.678872 | 1.3:+24804430 | MS.gene063249:CDS |
| ATTGGAACATGAAAACCTGG+TGG | 0.678986 | 1.3:-24804624 | None:intergenic |
| TGTGAATTTGGCAAACCATG+TGG | 0.742651 | 1.3:-24804735 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGAAATCAATTTTCTTGAT+AGG | - | chr1.3:24804837-24804856 | None:intergenic | 20.0% |
| !!! | GAAAATTGATTTCATTGATT+TGG | + | chr1.3:24804842-24804861 | MS.gene063249:CDS | 20.0% |
| ! | TAGAACCAACATTTAGAAAA+TGG | - | chr1.3:24804704-24804723 | None:intergenic | 25.0% |
| ! | TTTAGAAAATGGTTTCTTCT+TGG | - | chr1.3:24804693-24804712 | None:intergenic | 25.0% |
| !! | AGAAACCATTTTCTAAATGT+TGG | + | chr1.3:24804696-24804715 | MS.gene063249:CDS | 25.0% |
| !!! | ATTGATTTCATTGATTTGGT+TGG | + | chr1.3:24804846-24804865 | MS.gene063249:CDS | 25.0% |
| !!! | TTAATGTTTTGAGTGTCAAT+TGG | - | chr1.3:24804645-24804664 | None:intergenic | 25.0% |
| ATCTTCAAATGATGGTACTA+AGG | + | chr1.3:24804788-24804807 | MS.gene063249:CDS | 30.0% | |
| CAAATTCACATGTTTACCAA+TGG | + | chr1.3:24804748-24804767 | MS.gene063249:CDS | 30.0% | |
| CAAGAAGAATCTTCAAATGA+TGG | + | chr1.3:24804780-24804799 | MS.gene063249:CDS | 30.0% | |
| CATTGGTAAACATGTGAATT+TGG | - | chr1.3:24804750-24804769 | None:intergenic | 30.0% | |
| CTCAAAACATTAATGATGCT+AGG | + | chr1.3:24804652-24804671 | MS.gene063249:CDS | 30.0% | |
| AAATGGTTTCTTCTTGGTTG+TGG | - | chr1.3:24804687-24804706 | None:intergenic | 35.0% | |
| AGTGCTAAATCCAATGTTTG+AGG | - | chr1.3:24804894-24804913 | None:intergenic | 35.0% | |
| CTTGAGTGCTTATGAAAACT+CGG | - | chr1.3:24804494-24804513 | None:intergenic | 35.0% | |
| GATAAGAAGACTCTATTCGA+CGG | + | chr1.3:24804560-24804579 | MS.gene063249:CDS | 35.0% | |
| TCAATTGGAACATGAAAACC+TGG | - | chr1.3:24804630-24804649 | None:intergenic | 35.0% | |
| ! | TGGTAATGATGATGACAACT+CGG | + | chr1.3:24804866-24804885 | MS.gene063249:CDS | 35.0% |
| ATTGGAACATGAAAACCTGG+TGG | - | chr1.3:24804627-24804646 | None:intergenic | 40.0% | |
| CTTCTTGTAGATGCTTCCAT+TGG | - | chr1.3:24804767-24804786 | None:intergenic | 40.0% | |
| GAGAAGGAAATTGCACTTGA+GGG | - | chr1.3:24804596-24804615 | None:intergenic | 40.0% | |
| GAGGCATGATGTGTTAAACT+CGG | + | chr1.3:24804449-24804468 | MS.gene063249:CDS | 40.0% | |
| TCTATGCAACTTACACCACA+TGG | + | chr1.3:24804720-24804739 | MS.gene063249:CDS | 40.0% | |
| TGAGAAGGAAATTGCACTTG+AGG | - | chr1.3:24804597-24804616 | None:intergenic | 40.0% | |
| TGTGAATTTGGCAAACCATG+TGG | - | chr1.3:24804738-24804757 | None:intergenic | 40.0% | |
| TTGGAACATGAAAACCTGGT+GGG | - | chr1.3:24804626-24804645 | None:intergenic | 40.0% | |
| ! | GGTAATGATGATGACAACTC+GGG | + | chr1.3:24804867-24804886 | MS.gene063249:CDS | 40.0% |
| AACTCACATCGAGTCTCACA+TGG | + | chr1.3:24804521-24804540 | MS.gene063249:CDS | 45.0% | |
| GGTTTCTTCTTGGTTGTGGT+TGG | - | chr1.3:24804683-24804702 | None:intergenic | 45.0% | |
| ! | CCTCGTGTTACTAAAGCCAT+TGG | - | chr1.3:24804433-24804452 | None:intergenic | 45.0% |
| !! | CCAATGGCTTTAGTAACACG+AGG | + | chr1.3:24804430-24804449 | MS.gene063249:CDS | 45.0% |
| AGTCTCACATGGAGCACGAA+GGG | + | chr1.3:24804532-24804551 | MS.gene063249:CDS | 50.0% | |
| CAACTCGGGTCCTCAAACAT+TGG | + | chr1.3:24804881-24804900 | MS.gene063249:CDS | 50.0% | |
| CCTGGTGGGATACAATGAGA+AGG | - | chr1.3:24804612-24804631 | None:intergenic | 50.0% | |
| CCTTCTCATTGTATCCCACC+AGG | + | chr1.3:24804609-24804628 | MS.gene063249:CDS | 50.0% | |
| GAGTCTCACATGGAGCACGA+AGG | + | chr1.3:24804531-24804550 | MS.gene063249:CDS | 55.0% | |
| GTCTCACATGGAGCACGAAG+GGG | + | chr1.3:24804533-24804552 | MS.gene063249:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 24804426 | 24804923 | 24804426 | ID=MS.gene063249 |
| chr1.3 | mRNA | 24804426 | 24804923 | 24804426 | ID=MS.gene063249.t1;Parent=MS.gene063249 |
| chr1.3 | exon | 24804426 | 24804923 | 24804426 | ID=MS.gene063249.t1.exon1;Parent=MS.gene063249.t1 |
| chr1.3 | CDS | 24804426 | 24804923 | 24804426 | ID=cds.MS.gene063249.t1;Parent=MS.gene063249.t1 |
| Gene Sequence |
| Protein sequence |
