Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063286.t1 | XP_003589158.1 | 97.6 | 125 | 3 | 0 | 1 | 125 | 1 | 125 | 6.20E-63 | 250 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063286.t1 | Q6X7J9 | 57.6 | 132 | 40 | 6 | 1 | 124 | 1 | 124 | 3.5e-30 | 132.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063286.t1 | G7I2E0 | 97.6 | 125 | 3 | 0 | 1 | 125 | 1 | 125 | 4.5e-63 | 250.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene063286.t1 | TF | HB-WOX |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063286.t1 | MTR_1g019130 | 93.846 | 130 | 8 | 0 | 1 | 130 | 1 | 130 | 1.11e-88 | 257 |
| MS.gene063286.t1 | MTR_1g019130 | 93.846 | 130 | 8 | 0 | 1 | 130 | 1 | 130 | 5.47e-88 | 256 |
| MS.gene063286.t1 | MTR_7g060630 | 65.000 | 40 | 14 | 0 | 86 | 125 | 4 | 43 | 3.47e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063286.t1 | AT1G46480 | 55.072 | 138 | 46 | 6 | 1 | 130 | 1 | 130 | 1.02e-40 | 134 |
| MS.gene063286.t1 | AT1G46480 | 55.072 | 138 | 46 | 6 | 1 | 130 | 1 | 130 | 6.70e-40 | 134 |
| MS.gene063286.t1 | AT3G18010 | 32.773 | 119 | 70 | 2 | 6 | 124 | 2 | 110 | 3.95e-11 | 59.3 |
Find 30 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAATTCACACGTGGATTT+TGG | 0.121803 | 1.4:-4019016 | MS.gene063286:CDS |
| ACTAATTCTAGGAGCACTTT+CGG | 0.300053 | 1.4:+4018860 | None:intergenic |
| CTAATTCTAGGAGCACTTTC+GGG | 0.314910 | 1.4:+4018861 | None:intergenic |
| TGTTCTATTTGTTGTGCATT+TGG | 0.328080 | 1.4:+4018273 | None:intergenic |
| ACAAGAACAAATAGGAATAT+TGG | 0.328145 | 1.4:-4018325 | MS.gene063286:CDS |
| GAGGAAGAAAGACTAATTCT+AGG | 0.342909 | 1.4:+4018849 | None:intergenic |
| GGATTCCACCTTGTCCCTCC+TGG | 0.366142 | 1.4:+4018351 | None:intergenic |
| ATTCCTATTTGTTCTTGTGT+TGG | 0.415008 | 1.4:+4018330 | None:intergenic |
| AATCCAACACAAGAACAAAT+AGG | 0.426122 | 1.4:-4018333 | MS.gene063286:CDS |
| TAGGTTGAAACGCATATTCC+AGG | 0.450427 | 1.4:-4018369 | MS.gene063286:CDS |
| TCCACCTTCACCGCAAAGCC+AGG | 0.461999 | 1.4:-4018808 | MS.gene063286:intron |
| GGTGTTAGAAGTAACATTAT+CGG | 0.465029 | 1.4:+4018920 | None:intergenic |
| CATGAACCTTCCCTCACACT+TGG | 0.520457 | 1.4:-4018990 | MS.gene063286:CDS |
| TGAACCTGGCTTTGCGGTGA+AGG | 0.521265 | 1.4:+4018804 | None:intergenic |
| TGAGATCAAAAGAAAGAGAA+GGG | 0.531578 | 1.4:+4018898 | None:intergenic |
| AGTATATGAACCTGGCTTTG+CGG | 0.544738 | 1.4:+4018798 | None:intergenic |
| ATCTTTCTTGTTATCATCAG+AGG | 0.556963 | 1.4:+4018830 | None:intergenic |
| CGCATATTCCAGGAGGGACA+AGG | 0.565252 | 1.4:-4018359 | MS.gene063286:CDS |
| TTGAGATCAAAAGAAAGAGA+AGG | 0.574637 | 1.4:+4018897 | None:intergenic |
| AACGCTTGCAACCAAGTGTG+AGG | 0.582297 | 1.4:+4018979 | None:intergenic |
| ACCTGGCTTTGCGGTGAAGG+TGG | 0.590968 | 1.4:+4018807 | None:intergenic |
| TTGGAGATGTTATACAGAGG+TGG | 0.611718 | 1.4:-4018306 | MS.gene063286:CDS |
| ACGCTTGCAACCAAGTGTGA+GGG | 0.656705 | 1.4:+4018980 | None:intergenic |
| TTGAAACGCATATTCCAGGA+GGG | 0.657126 | 1.4:-4018365 | MS.gene063286:CDS |
| GAGATCAAAAGAAAGAGAAG+GGG | 0.671823 | 1.4:+4018899 | None:intergenic |
| TTGCAACCAAGTGTGAGGGA+AGG | 0.674259 | 1.4:+4018984 | None:intergenic |
| GTTGAAACGCATATTCCAGG+AGG | 0.708848 | 1.4:-4018366 | MS.gene063286:CDS |
| ATATTGGAGATGTTATACAG+AGG | 0.712720 | 1.4:-4018309 | MS.gene063286:CDS |
| AAAGTGCATCAATTCACACG+TGG | 0.742454 | 1.4:-4019023 | MS.gene063286:CDS |
| ATATTCCAGGAGGGACAAGG+TGG | 0.756182 | 1.4:-4018356 | MS.gene063286:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAATAAATAAATAAATT+AGG | - | chr1.4:4018389-4018408 | MS.gene063286:intron | 0.0% |
| !! | AAAAATAAATAAATAAATTA+GGG | - | chr1.4:4018390-4018409 | MS.gene063286:intron | 0.0% |
| !!! | AATACATTGTTTATTTTTTT+AGG | + | chr1.4:4018348-4018367 | None:intergenic | 10.0% |
| !!! | ATAATACTAATAAAGTATTT+TGG | - | chr1.4:4017495-4017514 | MS.gene063286:intron | 10.0% |
| !!! | ATACATTGTTTATTTTTTTA+GGG | + | chr1.4:4018347-4018366 | None:intergenic | 10.0% |
| !! | ACAGACTTAATTAAAAAAAA+TGG | + | chr1.4:4017340-4017359 | None:intergenic | 15.0% |
| !! | ATAAAAAGTTATTATTGCAT+TGG | + | chr1.4:4018013-4018032 | None:intergenic | 15.0% |
| !! | GATTTAAAAATTTAGATTCA+GGG | - | chr1.4:4018508-4018527 | MS.gene063286:intron | 15.0% |
| !!! | CATTTTGAAACAAATTTTTT+TGG | + | chr1.4:4017973-4017992 | None:intergenic | 15.0% |
| !! | AAGCATGAAAAACAAAATAA+AGG | - | chr1.4:4018621-4018640 | MS.gene063286:intron | 20.0% |
| !! | AATAAAGGACAAATACATTA+AGG | - | chr1.4:4018636-4018655 | MS.gene063286:intron | 20.0% |
| !! | ATTAACTTCATGTAACATAT+AGG | - | chr1.4:4017452-4017471 | MS.gene063286:intron | 20.0% |
| !! | GGATTTAAAAATTTAGATTC+AGG | - | chr1.4:4018507-4018526 | MS.gene063286:intron | 20.0% |
| !! | TCATGTAAGTACAATTTATA+TGG | - | chr1.4:4018119-4018138 | MS.gene063286:intron | 20.0% |
| !! | TCTATTATTTGTGTATGTAT+AGG | - | chr1.4:4017703-4017722 | MS.gene063286:intron | 20.0% |
| !! | TTAACTTCATGTAACATATA+GGG | - | chr1.4:4017453-4017472 | MS.gene063286:intron | 20.0% |
| !! | TTTATTATTGAATTAAGAGC+AGG | + | chr1.4:4018978-4018997 | None:intergenic | 20.0% |
| !!! | AAATACTAACTTCTAAAAGT+AGG | + | chr1.4:4018179-4018198 | None:intergenic | 20.0% |
| !!! | ATATAGATACATCTGAAATT+CGG | + | chr1.4:4017540-4017559 | None:intergenic | 20.0% |
| !!! | ATTTTTAATGCAACTGAATT+TGG | + | chr1.4:4017910-4017929 | None:intergenic | 20.0% |
| !!! | GTGTTATTAGAAGAAATTTT+AGG | + | chr1.4:4017152-4017171 | None:intergenic | 20.0% |
| !!! | TAAATCCAAGATTTTGTAAA+TGG | + | chr1.4:4018494-4018513 | None:intergenic | 20.0% |
| !!! | TTTAGAGCTAACTTTAAAAT+TGG | - | chr1.4:4018257-4018276 | MS.gene063286:CDS | 20.0% |
| ! | ACAAGAACAAATAGGAATAT+TGG | - | chr1.4:4017766-4017785 | MS.gene063286:intron | 25.0% |
| ! | ATCTACCATTTACAAAATCT+TGG | - | chr1.4:4018486-4018505 | MS.gene063286:intron | 25.0% |
| ! | TAAATTCTCAATGAAGTTTG+TGG | + | chr1.4:4018446-4018465 | None:intergenic | 25.0% |
| !! | TATGGTAAAAGAGTGAATTT+TGG | - | chr1.4:4018137-4018156 | MS.gene063286:intron | 25.0% |
| !!! | ACTAATAAAGTATTTTGGTC+CGG | - | chr1.4:4017500-4017519 | MS.gene063286:intron | 25.0% |
| !!! | CACTCATTTTTTTTACGATA+AGG | - | chr1.4:4018531-4018550 | MS.gene063286:intron | 25.0% |
| !!! | TCGTTTAAAAGCTTTTGATT+TGG | - | chr1.4:4017653-4017672 | MS.gene063286:intron | 25.0% |
| !!! | TGCTTTTAGTTTTGTCATAT+CGG | + | chr1.4:4018606-4018625 | None:intergenic | 25.0% |
| !!! | TTTTAGGGAACTAAATACAT+TGG | + | chr1.4:4018332-4018351 | None:intergenic | 25.0% |
| AAGGGAAAAATGTGTTCTAT+TGG | - | chr1.4:4017876-4017895 | MS.gene063286:intron | 30.0% | |
| AATCCAACACAAGAACAAAT+AGG | - | chr1.4:4017758-4017777 | MS.gene063286:intron | 30.0% | |
| ACAAATACATTAAGGCTGTT+TGG | - | chr1.4:4018644-4018663 | MS.gene063286:intron | 30.0% | |
| ATATTGGAGATGTTATACAG+AGG | - | chr1.4:4017782-4017801 | MS.gene063286:intron | 30.0% | |
| ATTCCTATTTGTTCTTGTGT+TGG | + | chr1.4:4017764-4017783 | None:intergenic | 30.0% | |
| GGTGTTAGAAGTAACATTAT+CGG | + | chr1.4:4017174-4017193 | None:intergenic | 30.0% | |
| TGAGATCAAAAGAAAGAGAA+GGG | + | chr1.4:4017196-4017215 | None:intergenic | 30.0% | |
| TTAACTTCATGTTAGCATCA+TGG | - | chr1.4:4018914-4018933 | MS.gene063286:CDS | 30.0% | |
| TTGAGATCAAAAGAAAGAGA+AGG | + | chr1.4:4017197-4017216 | None:intergenic | 30.0% | |
| ! | AATGTGTTCTATTGGTTGTA+TGG | - | chr1.4:4017884-4017903 | MS.gene063286:intron | 30.0% |
| ! | ATCTTTCTTGTTATCATCAG+AGG | + | chr1.4:4017264-4017283 | None:intergenic | 30.0% |
| !! | TGTTCTATTTGTTGTGCATT+TGG | + | chr1.4:4017821-4017840 | None:intergenic | 30.0% |
| !!! | CTAATAAAGTATTTTGGTCC+GGG | - | chr1.4:4017501-4017520 | MS.gene063286:intron | 30.0% |
| ACAGTTCAACTTAGCAAGTA+CGG | - | chr1.4:4017845-4017864 | MS.gene063286:intron | 35.0% | |
| AGCAAGTACGGTAAAATTGA+AGG | - | chr1.4:4017857-4017876 | MS.gene063286:intron | 35.0% | |
| GAGATCAAAAGAAAGAGAAG+GGG | + | chr1.4:4017195-4017214 | None:intergenic | 35.0% | |
| GAGGAAGAAAGACTAATTCT+AGG | + | chr1.4:4017245-4017264 | None:intergenic | 35.0% | |
| GCAAGTACGGTAAAATTGAA+GGG | - | chr1.4:4017858-4017877 | MS.gene063286:intron | 35.0% | |
| GCTAACATGAAGTTAAACGT+GGG | + | chr1.4:4018910-4018929 | None:intergenic | 35.0% | |
| TAGAGCTTAGTATATGAACC+TGG | + | chr1.4:4017304-4017323 | None:intergenic | 35.0% | |
| TGCTAACATGAAGTTAAACG+TGG | + | chr1.4:4018911-4018930 | None:intergenic | 35.0% | |
| TGTCATATCGGATGATGTAA+GGG | + | chr1.4:4018594-4018613 | None:intergenic | 35.0% | |
| TGTGTACAAAACATACTAGC+AGG | - | chr1.4:4018072-4018091 | MS.gene063286:intron | 35.0% | |
| TTCATGTTAGCATCATGGTA+TGG | - | chr1.4:4018919-4018938 | MS.gene063286:CDS | 35.0% | |
| TTGTCATATCGGATGATGTA+AGG | + | chr1.4:4018595-4018614 | None:intergenic | 35.0% | |
| ! | ATCAATTCACACGTGGATTT+TGG | - | chr1.4:4017075-4017094 | MS.gene063286:intron | 35.0% |
| ! | GGTTTTCTCTAACCTTACAT+GGG | + | chr1.4:4018425-4018444 | None:intergenic | 35.0% |
| ! | GTTTTCTCTAACCTTACATG+GGG | + | chr1.4:4018424-4018443 | None:intergenic | 35.0% |
| ! | TAAGCTAGCTTATAGCTTGT+TGG | - | chr1.4:4018676-4018695 | MS.gene063286:intron | 35.0% |
| ! | TCAATTCACACGTGGATTTT+GGG | - | chr1.4:4017076-4017095 | MS.gene063286:intron | 35.0% |
| ! | TGGTTTTCTCTAACCTTACA+TGG | + | chr1.4:4018426-4018445 | None:intergenic | 35.0% |
| ! | TTCGGTTCAACAATTTTTCC+CGG | + | chr1.4:4017522-4017541 | None:intergenic | 35.0% |
| !! | AAGCGACACTCATTTTGAAA+CGG | + | chr1.4:4017947-4017966 | None:intergenic | 35.0% |
| !! | ACTAATTCTAGGAGCACTTT+CGG | + | chr1.4:4017234-4017253 | None:intergenic | 35.0% |
| !! | GAAGAAATTTTAGGTGCAAG+AGG | + | chr1.4:4017143-4017162 | None:intergenic | 35.0% |
| !! | GGGCGATTTTATTTCTCATT+TGG | + | chr1.4:4018573-4018592 | None:intergenic | 35.0% |
| AAAGTGCATCAATTCACACG+TGG | - | chr1.4:4017068-4017087 | MS.gene063286:intron | 40.0% | |
| AATTATACCACCCTACTGCA+TGG | + | chr1.4:4017367-4017386 | None:intergenic | 40.0% | |
| AGTAACTCTCGAGTCTCATA+TGG | + | chr1.4:4017604-4017623 | None:intergenic | 40.0% | |
| CGAGTCTCATATGGAAAAAG+AGG | + | chr1.4:4017595-4017614 | None:intergenic | 40.0% | |
| GGGCAGTTGAGTAAGAATAT+GGG | + | chr1.4:4018890-4018909 | None:intergenic | 40.0% | |
| GTCATATCGGATGATGTAAG+GGG | + | chr1.4:4018593-4018612 | None:intergenic | 40.0% | |
| TGGGCAGTTGAGTAAGAATA+TGG | + | chr1.4:4018891-4018910 | None:intergenic | 40.0% | |
| TTGAAACGCATATTCCAGGA+GGG | - | chr1.4:4017726-4017745 | MS.gene063286:intron | 40.0% | |
| TTGGAGATGTTATACAGAGG+TGG | - | chr1.4:4017785-4017804 | MS.gene063286:intron | 40.0% | |
| ! | TAGGTTGAAACGCATATTCC+AGG | - | chr1.4:4017722-4017741 | MS.gene063286:intron | 40.0% |
| !! | AGTATATGAACCTGGCTTTG+CGG | + | chr1.4:4017296-4017315 | None:intergenic | 40.0% |
| !! | CTAATTCTAGGAGCACTTTC+GGG | + | chr1.4:4017233-4017252 | None:intergenic | 40.0% |
| !! | GGATTAGCTTATAAGCTCGT+TGG | - | chr1.4:4018697-4018716 | MS.gene063286:intron | 40.0% |
| !!! | GACACTCATTTTGAAACGGA+TGG | + | chr1.4:4017943-4017962 | None:intergenic | 40.0% |
| ACCAGATATTCTGCGACAGA+GGG | - | chr1.4:4018043-4018062 | MS.gene063286:intron | 45.0% | |
| GGATGAAAACGTGACGTGTT+TGG | - | chr1.4:4018718-4018737 | MS.gene063286:intron | 45.0% | |
| GTTGAAACGCATATTCCAGG+AGG | - | chr1.4:4017725-4017744 | MS.gene063286:intron | 45.0% | |
| TACCAGATATTCTGCGACAG+AGG | - | chr1.4:4018042-4018061 | MS.gene063286:intron | 45.0% | |
| !! | AAAATAAATAAATAAATTAG+GGG | - | chr1.4:4018391-4018410 | MS.gene063286:intron | 5.0% |
| !! | AATTAAACTAAATTAATAAA+AGG | + | chr1.4:4018846-4018865 | None:intergenic | 5.0% |
| AACGCTTGCAACCAAGTGTG+AGG | + | chr1.4:4017115-4017134 | None:intergenic | 50.0% | |
| ACGCTTGCAACCAAGTGTGA+GGG | + | chr1.4:4017114-4017133 | None:intergenic | 50.0% | |
| AGTCTGTCCATCCATGCAGT+AGG | - | chr1.4:4017353-4017372 | MS.gene063286:intron | 50.0% | |
| ATACCACCCTACTGCATGGA+TGG | + | chr1.4:4017363-4017382 | None:intergenic | 50.0% | |
| ATATTCCAGGAGGGACAAGG+TGG | - | chr1.4:4017735-4017754 | MS.gene063286:intron | 50.0% | |
| CATGAACCTTCCCTCACACT+TGG | - | chr1.4:4017101-4017120 | MS.gene063286:intron | 50.0% | |
| GTCTGTCCATCCATGCAGTA+GGG | - | chr1.4:4017354-4017373 | MS.gene063286:intron | 50.0% | |
| TCCCTCTGTCGCAGAATATC+TGG | + | chr1.4:4018047-4018066 | None:intergenic | 50.0% | |
| TTGCAACCAAGTGTGAGGGA+AGG | + | chr1.4:4017110-4017129 | None:intergenic | 50.0% | |
| CGCATATTCCAGGAGGGACA+AGG | - | chr1.4:4017732-4017751 | MS.gene063286:intron | 55.0% | |
| TGTCCATCCATGCAGTAGGG+TGG | - | chr1.4:4017357-4017376 | MS.gene063286:intron | 55.0% | |
| !! | TGAACCTGGCTTTGCGGTGA+AGG | + | chr1.4:4017290-4017309 | None:intergenic | 55.0% |
| GGATTCCACCTTGTCCCTCC+TGG | + | chr1.4:4017743-4017762 | None:intergenic | 60.0% | |
| TCCACCTTCACCGCAAAGCC+AGG | - | chr1.4:4017283-4017302 | MS.gene063286:intron | 60.0% | |
| ! | GGGGAAGCACACCCCATGTA+AGG | - | chr1.4:4018410-4018429 | MS.gene063286:intron | 60.0% |
| !! | ACCTGGCTTTGCGGTGAAGG+TGG | + | chr1.4:4017287-4017306 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 4017065 | 4019048 | 4017065 | ID=MS.gene063286 |
| chr1.4 | mRNA | 4017065 | 4019048 | 4017065 | ID=MS.gene063286.t1;Parent=MS.gene063286 |
| chr1.4 | exon | 4018809 | 4019048 | 4018809 | ID=MS.gene063286.t1.exon1;Parent=MS.gene063286.t1 |
| chr1.4 | CDS | 4018809 | 4019048 | 4018809 | ID=cds.MS.gene063286.t1;Parent=MS.gene063286.t1 |
| chr1.4 | exon | 4018252 | 4018388 | 4018252 | ID=MS.gene063286.t1.exon2;Parent=MS.gene063286.t1 |
| chr1.4 | CDS | 4018252 | 4018388 | 4018252 | ID=cds.MS.gene063286.t1;Parent=MS.gene063286.t1 |
| chr1.4 | exon | 4017065 | 4017086 | 4017065 | ID=MS.gene063286.t1.exon3;Parent=MS.gene063286.t1 |
| chr1.4 | CDS | 4017065 | 4017086 | 4017065 | ID=cds.MS.gene063286.t1;Parent=MS.gene063286.t1 |
| Gene Sequence |
| Protein sequence |
