Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06351.t1 | XP_003602688.2 | 65.2 | 296 | 44 | 5 | 1 | 258 | 190 | 464 | 8.30E-88 | 333.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06351.t1 | Q84TI6 | 44.1 | 299 | 103 | 12 | 1 | 258 | 195 | 470 | 1.1e-43 | 178.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06351.t1 | G7J2M4 | 65.2 | 296 | 44 | 5 | 1 | 258 | 190 | 464 | 6.0e-88 | 333.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06351.t1 | MTR_3g096960 | 64.865 | 296 | 45 | 5 | 1 | 258 | 190 | 464 | 2.59e-120 | 351 |
| MS.gene06351.t1 | MTR_8g080190 | 50.000 | 62 | 13 | 1 | 1 | 62 | 544 | 587 | 3.10e-13 | 69.3 |
| MS.gene06351.t1 | MTR_8g080190 | 50.000 | 62 | 13 | 1 | 1 | 62 | 544 | 587 | 3.37e-13 | 69.3 |
| MS.gene06351.t1 | MTR_4g103810 | 44.595 | 74 | 22 | 2 | 1 | 73 | 379 | 434 | 6.40e-13 | 68.6 |
| MS.gene06351.t1 | MTR_3g085650 | 48.387 | 62 | 14 | 1 | 1 | 62 | 575 | 618 | 1.08e-12 | 67.8 |
| MS.gene06351.t1 | MTR_3g085650 | 48.387 | 62 | 14 | 1 | 1 | 62 | 575 | 618 | 1.10e-12 | 67.8 |
| MS.gene06351.t1 | MTR_4g103810 | 44.595 | 74 | 22 | 2 | 1 | 73 | 269 | 324 | 1.12e-12 | 67.4 |
| MS.gene06351.t1 | MTR_3g085650 | 48.387 | 62 | 14 | 1 | 1 | 62 | 575 | 618 | 1.22e-12 | 67.8 |
| MS.gene06351.t1 | MTR_4g094430 | 40.541 | 74 | 25 | 2 | 2 | 74 | 164 | 219 | 1.65e-11 | 63.5 |
| MS.gene06351.t1 | MTR_3g085650 | 48.387 | 62 | 14 | 1 | 1 | 62 | 575 | 618 | 2.76e-11 | 63.5 |
| MS.gene06351.t1 | MTR_2g032060 | 40.541 | 74 | 25 | 2 | 2 | 74 | 164 | 219 | 3.96e-11 | 62.4 |
| MS.gene06351.t1 | MTR_5g010030 | 47.619 | 63 | 15 | 1 | 2 | 64 | 202 | 246 | 4.06e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06351.t1 | AT5G63610 | 44.518 | 301 | 99 | 16 | 1 | 258 | 195 | 470 | 3.04e-54 | 182 |
| MS.gene06351.t1 | AT1G67580 | 50.000 | 62 | 13 | 1 | 1 | 62 | 565 | 608 | 1.15e-12 | 67.8 |
| MS.gene06351.t1 | AT1G67580 | 50.000 | 62 | 13 | 1 | 1 | 62 | 565 | 608 | 1.15e-12 | 67.8 |
| MS.gene06351.t1 | AT1G76540 | 43.243 | 74 | 23 | 2 | 2 | 74 | 182 | 237 | 2.18e-12 | 66.2 |
| MS.gene06351.t1 | AT5G63370 | 51.613 | 62 | 12 | 1 | 1 | 62 | 461 | 504 | 6.68e-12 | 65.5 |
| MS.gene06351.t1 | AT5G63370 | 51.613 | 62 | 12 | 1 | 1 | 62 | 461 | 504 | 6.68e-12 | 65.5 |
| MS.gene06351.t1 | AT5G63370 | 51.613 | 62 | 12 | 1 | 1 | 62 | 461 | 504 | 7.80e-12 | 65.5 |
| MS.gene06351.t1 | AT5G63370 | 51.613 | 62 | 12 | 1 | 1 | 62 | 461 | 504 | 7.80e-12 | 65.5 |
| MS.gene06351.t1 | AT5G63370 | 51.613 | 62 | 12 | 1 | 1 | 62 | 461 | 504 | 7.80e-12 | 65.5 |
| MS.gene06351.t1 | AT5G63370 | 51.613 | 62 | 12 | 1 | 1 | 62 | 461 | 504 | 7.80e-12 | 65.5 |
| MS.gene06351.t1 | AT5G63370 | 51.613 | 62 | 12 | 1 | 1 | 62 | 461 | 504 | 7.80e-12 | 65.5 |
| MS.gene06351.t1 | AT5G63370 | 51.613 | 62 | 12 | 1 | 1 | 62 | 327 | 370 | 1.16e-11 | 64.7 |
| MS.gene06351.t1 | AT5G63370 | 51.613 | 62 | 12 | 1 | 1 | 62 | 327 | 370 | 1.43e-11 | 64.3 |
| MS.gene06351.t1 | AT5G63370 | 51.613 | 62 | 12 | 1 | 1 | 62 | 327 | 370 | 1.43e-11 | 64.3 |
| MS.gene06351.t1 | AT4G11330 | 47.619 | 63 | 15 | 1 | 2 | 64 | 204 | 248 | 5.18e-11 | 62.4 |
Find 85 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAAGAGGTAGGGGAAGTT+TGG | 0.207943 | 3.1:+77320504 | None:intergenic |
| CTTTGCATGCCAACATTAAT+TGG | 0.237085 | 3.1:+77313159 | None:intergenic |
| CGTGCTCCGGAGTTGCTTCT+TGG | 0.239612 | 3.1:-77322042 | MS.gene06351:CDS |
| GAAAGAGGTAGGGGAAGTTT+GGG | 0.340752 | 3.1:+77320505 | None:intergenic |
| TTCCACTGCTCATCCCTGCC+TGG | 0.352880 | 3.1:+77313103 | None:intergenic |
| TAGTGGATGTAATTATTGTT+GGG | 0.358965 | 3.1:-77321656 | MS.gene06351:CDS |
| TCGTCTGGAGCTGCTATTGC+TGG | 0.378419 | 3.1:-77313225 | MS.gene06351:CDS |
| ACTTCTGCACCTTGAAATAG+TGG | 0.381000 | 3.1:+77321463 | None:intergenic |
| TACCTGAGTCAGGTTTCGTC+TGG | 0.397060 | 3.1:-77313240 | MS.gene06351:intron |
| TTCTGCTGTGGAGGATATCC+TGG | 0.408739 | 3.1:+77312898 | None:intergenic |
| AACCATTTGGTACCGTGCTC+CGG | 0.411552 | 3.1:-77322055 | MS.gene06351:CDS |
| ACCTTGAAGCCACTATTTCA+AGG | 0.413740 | 3.1:-77321472 | MS.gene06351:CDS |
| TGCAGGTTGTTGTAACCATT+TGG | 0.414513 | 3.1:-77322068 | None:intergenic |
| TTAGTGGATGTAATTATTGT+TGG | 0.417455 | 3.1:-77321657 | MS.gene06351:intron |
| TTCAAAATGGAACCACAGCC+TGG | 0.419177 | 3.1:-77316801 | MS.gene06351:CDS |
| TAAAGAGACATTTAGATATG+TGG | 0.419701 | 3.1:-77321525 | MS.gene06351:intron |
| TCAGGTACCTGAAAAGGATT+TGG | 0.419922 | 3.1:+77321430 | None:intergenic |
| TTAGCATCAGGTACCTGAAA+AGG | 0.427384 | 3.1:+77321424 | None:intergenic |
| GGATATCCTGGCATTCCCAT+TGG | 0.442776 | 3.1:+77312910 | None:intergenic |
| GCAACGAGGTGTTCCACAAC+AGG | 0.446969 | 3.1:-77313058 | MS.gene06351:CDS |
| AACAAACCTGTTGCTGCTGA+TGG | 0.451452 | 3.1:+77313034 | None:intergenic |
| ACCTTGAAATAGTGGCTTCA+AGG | 0.455130 | 3.1:+77321471 | None:intergenic |
| GATATCCTGGCATTCCCATT+GGG | 0.462213 | 3.1:+77312911 | None:intergenic |
| GTGGAACACCTCGTTGCATT+GGG | 0.467473 | 3.1:+77313064 | None:intergenic |
| GTAATTATTGTTGGGGACTT+CGG | 0.469241 | 3.1:-77321648 | MS.gene06351:CDS |
| CGTTGCATTGGGATGCCAGC+AGG | 0.469966 | 3.1:+77313075 | None:intergenic |
| ACAGGCCCATCAGCAGCAAC+AGG | 0.477025 | 3.1:-77313040 | MS.gene06351:intron |
| CCTGCTGCAAAACATTCTGC+AGG | 0.485287 | 3.1:-77320431 | MS.gene06351:CDS |
| AAAGACCCAATGGGAATGCC+AGG | 0.486170 | 3.1:-77312916 | MS.gene06351:CDS |
| TCCACTGCTCATCCCTGCCT+GGG | 0.490929 | 3.1:+77313104 | None:intergenic |
| CCTTACTTCCGTCCAGGCTG+TGG | 0.498840 | 3.1:+77316789 | None:intergenic |
| TGTGAATTATCCCACACGTC+CGG | 0.498897 | 3.1:-77316475 | MS.gene06351:CDS |
| CAGAAATCAAGACGTATGTA+AGG | 0.498939 | 3.1:-77312880 | None:intergenic |
| TGCTGCTGATGGGCCTGTTG+TGG | 0.499210 | 3.1:+77313045 | None:intergenic |
| AAATCTGTGGTTGTGTCCAC+CGG | 0.500086 | 3.1:+77316456 | None:intergenic |
| GGCTAATCGTGGTGGAGTTA+TGG | 0.504456 | 3.1:-77320456 | MS.gene06351:CDS |
| TTGGCATGGTACAAGTGCAC+TGG | 0.505126 | 3.1:+77316512 | None:intergenic |
| CTCCGGAGCACGGTACCAAA+TGG | 0.509771 | 3.1:+77322053 | None:intergenic |
| TGTGGAACACCTCGTTGCAT+TGG | 0.511061 | 3.1:+77313063 | None:intergenic |
| TATAATCTCACATCCCAGGC+AGG | 0.512489 | 3.1:-77313117 | MS.gene06351:CDS |
| ATACGTCTTGATTTCTGCTG+TGG | 0.520190 | 3.1:+77312886 | None:intergenic |
| ACATTTAGATATGTGGGCTG+TGG | 0.524919 | 3.1:-77321518 | MS.gene06351:CDS |
| GCTATTGCTGGAAACATGCC+TGG | 0.525437 | 3.1:-77313213 | MS.gene06351:CDS |
| AGCAGTGGAATTAATCCTGC+TGG | 0.530273 | 3.1:-77313090 | MS.gene06351:CDS |
| AAAGAGACATTTAGATATGT+GGG | 0.531573 | 3.1:-77321524 | MS.gene06351:intron |
| AGTGGATGTAATTATTGTTG+GGG | 0.533882 | 3.1:-77321655 | MS.gene06351:CDS |
| ATGCCAACATTAATTGGTCG+AGG | 0.536101 | 3.1:+77313165 | None:intergenic |
| CGTCTTGATTTCTGCTGTGG+AGG | 0.537193 | 3.1:+77312889 | None:intergenic |
| CGGCTGTGATTGCTGCATGT+TGG | 0.539442 | 3.1:+77316422 | None:intergenic |
| GTGACAACATAGAAAGAGGT+AGG | 0.542479 | 3.1:+77320494 | None:intergenic |
| GACAACATAGAAAGAGGTAG+GGG | 0.545487 | 3.1:+77320496 | None:intergenic |
| AAAAGGCCTTACTTCCGTCC+AGG | 0.546934 | 3.1:+77316783 | None:intergenic |
| CTATTTGACGCATGACCACC+AGG | 0.548847 | 3.1:+77313195 | None:intergenic |
| ACAAACCTGTTGCTGCTGAT+GGG | 0.553904 | 3.1:+77313035 | None:intergenic |
| TTTAAGGATACCAACAGCGC+TGG | 0.554418 | 3.1:+77322005 | None:intergenic |
| GCACTTGTACCATGCCAACC+TGG | 0.555366 | 3.1:-77316507 | MS.gene06351:CDS |
| TAATTCACAAAAGCCTCTCC+AGG | 0.561125 | 3.1:+77316489 | None:intergenic |
| AAACATTATACCAGCGCTGT+TGG | 0.563837 | 3.1:-77322015 | MS.gene06351:intron |
| GGTTGTGTCCACCGGACGTG+TGG | 0.564371 | 3.1:+77316464 | None:intergenic |
| ACCACGATTAGCCACAAAAG+TGG | 0.565639 | 3.1:+77320466 | None:intergenic |
| AACTTATAATCTCACATCCC+AGG | 0.570901 | 3.1:-77313121 | MS.gene06351:CDS |
| GGTTGTCCCTTCAAAATCTG+TGG | 0.572004 | 3.1:+77316443 | None:intergenic |
| CATTTAGATATGTGGGCTGT+GGG | 0.572377 | 3.1:-77321517 | MS.gene06351:CDS |
| TGACAACATAGAAAGAGGTA+GGG | 0.572719 | 3.1:+77320495 | None:intergenic |
| GTCCCTCGACCAATTAATGT+TGG | 0.572871 | 3.1:-77313168 | MS.gene06351:CDS |
| TTGAGGAGAAAAGACCCAAT+GGG | 0.574208 | 3.1:-77312925 | MS.gene06351:CDS |
| AAATGGAACCACAGCCTGGA+CGG | 0.583599 | 3.1:-77316797 | MS.gene06351:CDS |
| TCCCAGGCAGGGATGAGCAG+TGG | 0.595368 | 3.1:-77313105 | MS.gene06351:CDS |
| AAAGCCTCTCCAGGTTGGCA+TGG | 0.595576 | 3.1:+77316498 | None:intergenic |
| GTTGAGGAGAAAAGACCCAA+TGG | 0.601461 | 3.1:-77312926 | MS.gene06351:CDS |
| GCTAGTGACAACATAGAAAG+AGG | 0.605070 | 3.1:+77320490 | None:intergenic |
| TCACAAAAGCCTCTCCAGGT+TGG | 0.606451 | 3.1:+77316493 | None:intergenic |
| TGGCATGGTACAAGTGCACT+GGG | 0.606512 | 3.1:+77316513 | None:intergenic |
| TGCAAAACATTCTGCAGGTG+CGG | 0.608072 | 3.1:-77320426 | MS.gene06351:intron |
| ATAATCTCACATCCCAGGCA+GGG | 0.616240 | 3.1:-77313116 | MS.gene06351:CDS |
| CAACATGCAGCAATCACAGC+CGG | 0.617560 | 3.1:-77316421 | MS.gene06351:intron |
| TGTACCATGCCAACCTGGAG+AGG | 0.622877 | 3.1:-77316502 | MS.gene06351:CDS |
| TGCCAACATTAATTGGTCGA+GGG | 0.628918 | 3.1:+77313166 | None:intergenic |
| CTTCGGTACTCATAATCCAA+AGG | 0.634082 | 3.1:-77321631 | MS.gene06351:intron |
| GTTGTGTCCACCGGACGTGT+GGG | 0.635167 | 3.1:+77316465 | None:intergenic |
| TTAGCTCCAAGAAGCAACTC+CGG | 0.639529 | 3.1:+77322036 | None:intergenic |
| CAAGAAGCAACTCCGGAGCA+CGG | 0.641447 | 3.1:+77322043 | None:intergenic |
| GAATTATCCCACACGTCCGG+TGG | 0.647048 | 3.1:-77316472 | MS.gene06351:CDS |
| ATTGCTGGAAACATGCCTGG+TGG | 0.745461 | 3.1:-77313210 | MS.gene06351:CDS |
| GCTGGCATCCCAATGCAACG+AGG | 0.747074 | 3.1:-77313072 | MS.gene06351:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 77312882 | 77322085 | 77312882 | ID=MS.gene06351 |
| chr3.1 | mRNA | 77312882 | 77322085 | 77312882 | ID=MS.gene06351.t1;Parent=MS.gene06351 |
| chr3.1 | exon | 77322016 | 77322085 | 77322016 | ID=MS.gene06351.t1.exon1;Parent=MS.gene06351.t1 |
| chr3.1 | CDS | 77322016 | 77322085 | 77322016 | ID=cds.MS.gene06351.t1;Parent=MS.gene06351.t1 |
| chr3.1 | exon | 77321632 | 77321675 | 77321632 | ID=MS.gene06351.t1.exon2;Parent=MS.gene06351.t1 |
| chr3.1 | CDS | 77321632 | 77321675 | 77321632 | ID=cds.MS.gene06351.t1;Parent=MS.gene06351.t1 |
| chr3.1 | exon | 77321438 | 77321542 | 77321438 | ID=MS.gene06351.t1.exon3;Parent=MS.gene06351.t1 |
| chr3.1 | CDS | 77321438 | 77321542 | 77321438 | ID=cds.MS.gene06351.t1;Parent=MS.gene06351.t1 |
| chr3.1 | exon | 77320427 | 77320537 | 77320427 | ID=MS.gene06351.t1.exon4;Parent=MS.gene06351.t1 |
| chr3.1 | CDS | 77320427 | 77320537 | 77320427 | ID=cds.MS.gene06351.t1;Parent=MS.gene06351.t1 |
| chr3.1 | exon | 77316795 | 77316827 | 77316795 | ID=MS.gene06351.t1.exon5;Parent=MS.gene06351.t1 |
| chr3.1 | CDS | 77316795 | 77316827 | 77316795 | ID=cds.MS.gene06351.t1;Parent=MS.gene06351.t1 |
| chr3.1 | exon | 77316422 | 77316559 | 77316422 | ID=MS.gene06351.t1.exon6;Parent=MS.gene06351.t1 |
| chr3.1 | CDS | 77316422 | 77316559 | 77316422 | ID=cds.MS.gene06351.t1;Parent=MS.gene06351.t1 |
| chr3.1 | exon | 77313041 | 77313250 | 77313041 | ID=MS.gene06351.t1.exon7;Parent=MS.gene06351.t1 |
| chr3.1 | CDS | 77313041 | 77313250 | 77313041 | ID=cds.MS.gene06351.t1;Parent=MS.gene06351.t1 |
| chr3.1 | exon | 77312882 | 77312947 | 77312882 | ID=MS.gene06351.t1.exon8;Parent=MS.gene06351.t1 |
| chr3.1 | CDS | 77312882 | 77312947 | 77312882 | ID=cds.MS.gene06351.t1;Parent=MS.gene06351.t1 |
| Gene Sequence |
| Protein sequence |
