AlfalfaGEDB | MS.gene063592

M. sativa cultivar XinJiangDaYe / MS.gene063592

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063592.t1	NP_173829.3	56.2	73	31	1	7	79	13	84	9.00E-12	79.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063592.t1	O48686	56.2	73	31	1	7	79	13	84	2.2e-14	79.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063592.t1	F4I962	56.2	73	31	1	7	79	13	84	6.5e-12	79.3

TFs/TRs:

Gene ID	Type	Classification
MS.gene063592.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063592.t1	MTR_1g105960	55.385	65	29	0	12	76	4	68	2.78e-18	72.8
MS.gene063592.t1	MTR_1g105955	53.846	65	30	0	12	76	98	162	3.25e-17	72.0
MS.gene063592.t1	MTR_1g105955	49.231	65	33	0	8	72	15	79	2.44e-14	64.3
MS.gene063592.t1	MTR_5g036670	51.351	74	35	1	7	80	42	114	6.14e-17	73.9
MS.gene063592.t1	MTR_6g008970	50.685	73	35	1	7	79	62	133	4.89e-16	71.2
MS.gene063592.t1	MTR_8g062700	51.351	74	35	1	7	80	41	113	8.12e-16	70.9
MS.gene063592.t1	MTR_8g062700	51.351	74	35	1	7	80	41	113	8.12e-16	70.9
MS.gene063592.t1	MTR_8g062350	47.887	71	36	1	6	76	12	81	2.76e-15	67.4
MS.gene063592.t1	MTR_1g109550	46.269	67	35	1	10	76	17	82	2.18e-14	63.9
MS.gene063592.t1	MTR_3g006065	52.239	67	32	0	8	74	90	156	4.44e-14	63.9
MS.gene063592.t1	MTR_3g006185	51.515	66	31	1	12	76	101	166	8.55e-14	62.8
MS.gene063592.t1	MTR_3g006150	55.882	68	28	2	8	74	90	156	8.81e-14	62.8
MS.gene063592.t1	MTR_4g133300	52.239	67	32	0	12	78	175	241	9.69e-14	63.9
MS.gene063592.t1	MTR_4g133300	41.333	75	40	2	7	81	11	81	2.73e-11	57.4
MS.gene063592.t1	MTR_4g133470	52.239	67	32	0	12	78	175	241	1.14e-13	63.9
MS.gene063592.t1	MTR_4g133470	41.333	75	40	2	7	81	11	81	2.90e-11	57.4
MS.gene063592.t1	MTR_1g104940	47.761	67	35	0	12	78	17	83	2.76e-13	60.5
MS.gene063592.t1	MTR_2g097830	41.333	75	40	2	7	81	69	139	5.93e-13	60.5
MS.gene063592.t1	MTR_1g093310	45.455	77	35	2	2	78	3	72	1.35e-12	57.8
MS.gene063592.t1	MTR_1g091047	51.515	66	30	2	7	71	8	72	2.03e-12	57.0
MS.gene063592.t1	MTR_6g008995	45.833	72	38	1	8	79	37	107	5.03e-12	59.7
MS.gene063592.t1	MTR_3g006125	46.970	66	34	1	12	76	183	248	6.57e-12	58.9
MS.gene063592.t1	MTR_1g104960	39.474	76	46	0	7	82	97	172	9.76e-12	57.8
MS.gene063592.t1	MTR_1g104960	48.438	64	33	0	15	78	19	82	6.11e-11	55.5
MS.gene063592.t1	MTR_1g100260	47.945	73	34	2	13	81	28	100	2.31e-11	55.5
MS.gene063592.t1	MTR_1g105010	43.077	65	37	0	12	76	19	83	6.49e-11	54.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene063592.t1	AT1G24190	56.164	73	31	1	7	79	13	84	1.52e-18	78.6
MS.gene063592.t1	AT1G24190	56.164	73	31	1	7	79	13	84	1.57e-18	78.6
MS.gene063592.t1	AT1G24190	56.164	73	31	1	7	79	13	84	1.65e-18	78.6
MS.gene063592.t1	AT1G70060	54.167	72	32	1	7	78	13	83	7.78e-16	70.9
MS.gene063592.t1	AT1G70060	54.167	72	32	1	7	78	13	83	7.78e-16	70.9
MS.gene063592.t1	AT3G01320	51.389	72	34	1	7	78	56	126	1.29e-15	70.1
MS.gene063592.t1	AT3G01320	51.389	72	34	1	7	78	56	126	1.31e-15	70.1
MS.gene063592.t1	AT5G15020	50.685	73	35	1	7	79	51	122	2.08e-15	69.7
MS.gene063592.t1	AT5G15020	50.685	73	35	1	7	79	51	122	2.08e-15	69.7
MS.gene063592.t1	AT1G59890	50.000	72	35	1	8	79	45	115	3.51e-14	66.2
MS.gene063592.t1	AT5G15025	49.315	73	36	1	7	79	46	117	3.54e-14	62.8
MS.gene063592.t1	AT1G59890	50.000	72	35	1	8	79	45	115	3.83e-14	65.9
MS.gene063592.t1	AT1G59890	50.000	72	35	1	8	79	45	115	3.83e-14	65.9
MS.gene063592.t1	AT1G59890	50.000	72	35	1	8	79	45	115	3.83e-14	65.9
MS.gene063592.t1	AT1G70030	45.070	71	38	1	8	78	22	91	1.00e-13	61.6
MS.gene063592.t1	AT1G70030	45.070	71	38	1	8	78	22	91	2.36e-13	61.2
MS.gene063592.t1	AT1G70030	45.070	71	38	1	8	78	11	80	3.55e-13	61.2
MS.gene063592.t1	AT1G10450	46.479	71	37	1	8	78	84	153	1.43e-11	58.5
MS.gene063592.t1	AT1G24200	45.714	70	37	1	7	76	13	81	1.59e-11	57.4
MS.gene063592.t1	AT4G12020	45.070	71	37	2	8	78	308	376	1.76e-11	58.5
MS.gene063592.t1	AT4G12020	45.070	71	37	2	8	78	308	376	1.78e-11	58.5
MS.gene063592.t1	AT4G12020	45.070	71	37	2	8	78	308	376	1.78e-11	58.5
MS.gene063592.t1	AT4G12020	45.070	71	37	2	8	78	308	376	1.78e-11	58.5
MS.gene063592.t1	AT4G12020	45.070	71	37	2	8	78	241	309	1.78e-11	58.5
MS.gene063592.t1	AT4G12020	45.070	71	37	2	8	78	308	376	1.78e-11	58.5
MS.gene063592.t1	AT4G12020	45.070	71	37	2	8	78	308	376	1.78e-11	58.5
MS.gene063592.t1	AT4G12020	45.070	71	37	2	8	78	308	376	1.78e-11	58.5
MS.gene063592.t1	AT4G12020	45.070	71	37	2	8	78	308	376	1.78e-11	58.5
MS.gene063592.t1	AT1G10450	46.479	71	37	1	8	78	33	102	1.90e-11	58.5
MS.gene063592.t1	AT1G10450	46.479	71	37	1	8	78	33	102	1.90e-11	58.5
MS.gene063592.t1	AT4G12020	45.070	71	37	2	8	78	308	376	2.00e-11	58.2
MS.gene063592.t1	AT4G12020	45.070	71	37	2	8	78	308	376	2.00e-11	58.2

Find 19 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGGCATACTCACTTAATTT+TGG	0.125153	5.2:-65328591	MS.gene063592:CDS
CCAATGCATCGTCGCGGTTT+TGG	0.138304	5.2:+65328753	None:intergenic
AGAGTCAAGCAGCTGCTTAA+AGG	0.323406	5.2:-65328611	MS.gene063592:CDS
AGTATAATTTGATGTTCCTT+TGG	0.390609	5.2:+65328551	None:intergenic
GAGTCAAGCAGCTGCTTAAA+GGG	0.418662	5.2:-65328610	MS.gene063592:CDS
TAATTTGATGTTCCTTTGGC+AGG	0.455833	5.2:+65328555	None:intergenic
TGCATTGGCAGCGTTTCATA+AGG	0.456199	5.2:-65328738	MS.gene063592:CDS
CCAAAACCGCGACGATGCAT+TGG	0.481999	5.2:-65328753	MS.gene063592:CDS
TTGATGTTCCTTTGGCAGGA+AGG	0.538933	5.2:+65328559	None:intergenic
AAAACTCTCAAAAGATGTCA+AGG	0.541090	5.2:-65328666	MS.gene063592:CDS
CAAATTATACTTGAGACCAC+TGG	0.569052	5.2:-65328539	MS.gene063592:CDS
AGCTGCGTTTCAAGATCAGA+GGG	0.585156	5.2:-65328708	MS.gene063592:CDS
AAGCTGCGTTTCAAGATCAG+AGG	0.599823	5.2:-65328709	MS.gene063592:CDS
CAGCTGCTTGACTCTTGCCG+CGG	0.614341	5.2:+65328619	None:intergenic
ATTCAACACCTTCCTGCCAA+AGG	0.618562	5.2:-65328567	MS.gene063592:CDS
AATCATAAATTACTAACCAG+TGG	0.644366	5.2:+65328523	None:intergenic
AATTTACACAAGAGATGCCG+CGG	0.649048	5.2:-65328636	MS.gene063592:CDS
ACGCTGCCAATGCATCGTCG+CGG	0.755650	5.2:+65328747	None:intergenic
GCTGCGTTTCAAGATCAGAG+GGG	0.802684	5.2:-65328707	MS.gene063592:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAATAATTGATTTTAGTGA+AGG	+	chr5.2:65328554-65328573	None:intergenic	15.0%
!!!	ACTAAAATCAATTATTTTTC+TGG	-	chr5.2:65328556-65328575	MS.gene063592:CDS	15.0%
!!!	GTTTTATTATTCTGAATTAT+TGG	-	chr5.2:65328578-65328597	MS.gene063592:CDS	15.0%
!!!	TATACTGAATCATTAATTTT+CGG	-	chr5.2:65328608-65328627	MS.gene063592:CDS	15.0%
!	AGTATAATTTGATGTTCCTT+TGG	+	chr5.2:65328854-65328873	None:intergenic	25.0%
	AAAACTCTCAAAAGATGTCA+AGG	-	chr5.2:65328736-65328755	MS.gene063592:CDS	30.0%
	CAAATTATACTTGAGACCAC+TGG	-	chr5.2:65328863-65328882	MS.gene063592:intron	35.0%
	TAATTTGATGTTCCTTTGGC+AGG	+	chr5.2:65328850-65328869	None:intergenic	35.0%
!	AGGGCATACTCACTTAATTT+TGG	-	chr5.2:65328811-65328830	MS.gene063592:intron	35.0%
!	GGGCATACTCACTTAATTTT+GGG	-	chr5.2:65328812-65328831	MS.gene063592:intron	35.0%
!!	ATTGATTTTAGTGAAGGACG+AGG	+	chr5.2:65328548-65328567	None:intergenic	35.0%
	AATTTACACAAGAGATGCCG+CGG	-	chr5.2:65328766-65328785	MS.gene063592:intron	40.0%
	AAGCTGCGTTTCAAGATCAG+AGG	-	chr5.2:65328693-65328712	MS.gene063592:CDS	45.0%
	AGAGTCAAGCAGCTGCTTAA+AGG	-	chr5.2:65328791-65328810	MS.gene063592:intron	45.0%
	AGCTGCGTTTCAAGATCAGA+GGG	-	chr5.2:65328694-65328713	MS.gene063592:CDS	45.0%
	ATTCAACACCTTCCTGCCAA+AGG	-	chr5.2:65328835-65328854	MS.gene063592:intron	45.0%
	GAGTCAAGCAGCTGCTTAAA+GGG	-	chr5.2:65328792-65328811	MS.gene063592:intron	45.0%
	TTGATGTTCCTTTGGCAGGA+AGG	+	chr5.2:65328846-65328865	None:intergenic	45.0%
!	TGCATTGGCAGCGTTTCATA+AGG	-	chr5.2:65328664-65328683	MS.gene063592:CDS	45.0%
	GCTGCGTTTCAAGATCAGAG+GGG	-	chr5.2:65328695-65328714	MS.gene063592:CDS	50.0%
!	AATGCATCGTCGCGGTTTTG+GGG	+	chr5.2:65328650-65328669	None:intergenic	50.0%
!	CAATGCATCGTCGCGGTTTT+GGG	+	chr5.2:65328651-65328670	None:intergenic	50.0%
	CCAAAACCGCGACGATGCAT+TGG	-	chr5.2:65328649-65328668	MS.gene063592:CDS	55.0%
!	CCAATGCATCGTCGCGGTTT+TGG	+	chr5.2:65328652-65328671	None:intergenic	55.0%
!!	ATGCATCGTCGCGGTTTTGG+GGG	+	chr5.2:65328649-65328668	None:intergenic	55.0%
	ACGCTGCCAATGCATCGTCG+CGG	+	chr5.2:65328658-65328677	None:intergenic	60.0%
	CAGCTGCTTGACTCTTGCCG+CGG	+	chr5.2:65328786-65328805	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	65328535	65328889	65328535	ID=MS.gene063592
chr5.2	mRNA	65328535	65328889	65328535	ID=MS.gene063592.t1;Parent=MS.gene063592
chr5.2	exon	65328887	65328889	65328887	ID=MS.gene063592.t1.exon1;Parent=MS.gene063592.t1
chr5.2	CDS	65328887	65328889	65328887	ID=cds.MS.gene063592.t1;Parent=MS.gene063592.t1
chr5.2	exon	65328535	65328780	65328535	ID=MS.gene063592.t1.exon2;Parent=MS.gene063592.t1
chr5.2	CDS	65328535	65328780	65328535	ID=cds.MS.gene063592.t1;Parent=MS.gene063592.t1

Gene Sequence	>MS.gene063592 ATGGTATGCTCCTCGTCCTTCACTAAAATCAATTATTTTTCTGGTTTTATTATTCTGAATTATTGGAATTAGTTATACTGAATCATTAATTTTCGGAATAATGATGAAGATCCCCCAAAACCGCGACGATGCATTGGCAGCGTTTCATAAGGCTGTACAAGCTGCGTTTCAAGATCAGAGGGGAGATTACAATGAGCTTTTAAAACTCTCAAAAGATGTCAAGGATCAAAGAATTTACACAAGAGATGCCGCGGCAAGAGTCAAGCAGCTGCTTAAAGGGCATACTCACTTAATTTTGGGATTCAACACCTTCCTGCCAAAGGAACATCAAATTATACTTGAGACCACTGGTTAG
Protein sequence	>MS.gene063592.t1 MIPQNRDDALAAFHKAVQAAFQDQRGDYNELLKLSKDVKDQRIYTRDAAARVKQLLKGHTHLILGFNTFLPKEHQIILETTG