AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063628

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063628.t1 MTR_0402s0040 57.000 200 58 2 30 202 2 200 2.80e-64 220
MS.gene063628.t1 MTR_0402s0040 46.850 254 63 4 387 639 200 382 1.55e-55 196
MS.gene063628.t1 MTR_5g015480 54.598 174 46 3 10 156 18 185 1.42e-45 162
MS.gene063628.t1 MTR_1g086270 81.707 82 15 0 322 403 1 82 2.78e-43 151
MS.gene063628.t1 MTR_7g113010 58.219 146 48 1 20 152 423 568 2.25e-41 159
MS.gene063628.t1 MTR_6g051425 31.317 281 168 6 57 321 49 320 7.92e-38 144
MS.gene063628.t1 MTR_0558s0020 38.068 176 108 1 94 268 121 296 2.34e-36 138
MS.gene063628.t1 MTR_8g446020 35.795 176 112 1 94 268 81 256 6.24e-33 127
MS.gene063628.t1 MTR_6g090505 37.288 177 110 1 94 269 56 232 1.02e-31 127
MS.gene063628.t1 MTR_6g017200 47.015 134 40 2 20 122 20 153 3.73e-28 110
MS.gene063628.t1 MTR_1g057920 50.413 121 33 1 23 116 3 123 5.22e-28 109
MS.gene063628.t1 MTR_2g070590 52.212 113 27 1 45 130 8 120 7.94e-28 108
MS.gene063628.t1 MTR_0137s0040 46.429 140 48 3 19 158 1 113 1.10e-27 108
MS.gene063628.t1 MTR_1g050985 80.000 60 12 0 97 156 39 98 1.40e-25 101
MS.gene063628.t1 MTR_1g070355 29.461 241 137 4 53 262 71 309 8.60e-24 106
MS.gene063628.t1 MTR_1g083640 31.973 147 99 1 94 239 136 282 1.78e-21 95.5
MS.gene063628.t1 MTR_4g035820 29.617 287 159 9 25 272 42 324 3.39e-19 92.4
MS.gene063628.t1 MTR_0337s0040 30.405 148 102 1 87 233 75 222 1.23e-18 85.9
MS.gene063628.t1 MTR_7g446520 30.837 227 142 6 57 270 91 315 9.67e-18 85.5
MS.gene063628.t1 MTR_7g446480 33.951 162 104 2 103 262 140 300 2.00e-16 81.3
MS.gene063628.t1 MTR_7g446510 33.333 162 105 2 103 262 155 315 7.26e-16 79.7
MS.gene063628.t1 MTR_6g022070 28.492 179 123 1 322 500 1 174 7.37e-16 79.7
MS.gene063628.t1 MTR_1g008290 33.333 138 86 3 85 221 9 141 9.31e-15 72.4
MS.gene063628.t1 MTR_7g074790 32.759 174 70 3 51 197 7 160 2.43e-14 73.2
MS.gene063628.t1 MTR_0291s0060 57.143 49 21 0 32 80 13 61 1.47e-11 61.6
MS.gene063628.t1 MTR_5g039760 27.805 205 93 5 48 223 4 182 1.73e-11 63.9
MS.gene063628.t1 MTR_5g076070 23.684 228 139 5 60 256 59 282 2.33e-11 65.5
MS.gene063628.t1 MTR_5g045210 40.000 75 45 0 559 633 20 94 2.72e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063628.t1 AT4G38180 34.918 610 349 10 42 609 58 661 7.86e-116 364
MS.gene063628.t1 AT4G15090 31.759 614 372 11 50 627 47 649 4.64e-95 311
MS.gene063628.t1 AT4G15090 31.759 614 372 11 50 627 47 649 4.64e-95 311
MS.gene063628.t1 AT4G15090 31.759 614 372 11 50 627 47 649 4.64e-95 311
MS.gene063628.t1 AT4G15090 31.759 614 372 11 50 627 47 649 4.64e-95 311
MS.gene063628.t1 AT4G15090 31.759 614 372 11 50 627 47 649 4.64e-95 311
MS.gene063628.t1 AT3G22170 30.317 630 357 12 49 627 59 657 2.10e-88 293
MS.gene063628.t1 AT3G22170 30.317 630 357 12 49 627 59 657 2.10e-88 293
MS.gene063628.t1 AT1G76320 32.459 610 346 15 58 627 1 584 2.23e-86 284
MS.gene063628.t1 AT1G76320 32.459 610 346 17 58 627 1 584 6.83e-86 284
MS.gene063628.t1 AT1G76320 32.459 610 346 17 58 627 1 584 6.83e-86 284
MS.gene063628.t1 AT2G27110 32.378 593 362 13 54 627 49 621 8.25e-86 286
MS.gene063628.t1 AT2G27110 32.378 593 362 13 54 627 49 621 8.25e-86 286
MS.gene063628.t1 AT1G76320 32.459 610 346 17 58 627 1 584 9.62e-86 283
MS.gene063628.t1 AT2G27110 34.965 429 265 8 206 627 55 476 6.59e-77 259
MS.gene063628.t1 AT1G10240 26.625 646 426 12 34 638 26 664 1.14e-69 239
MS.gene063628.t1 AT1G10240 26.625 646 426 12 34 638 26 664 1.14e-69 239
MS.gene063628.t1 AT3G06250 28.772 570 346 12 54 600 190 722 2.14e-69 240
MS.gene063628.t1 AT3G06250 28.772 570 346 12 54 600 190 722 2.14e-69 240
MS.gene063628.t1 AT3G06250 28.772 570 346 12 54 600 190 722 2.14e-69 240
MS.gene063628.t1 AT2G32250 26.721 610 373 10 58 639 42 605 2.06e-68 238
MS.gene063628.t1 AT2G32250 26.721 610 373 10 58 639 1 564 2.38e-68 237
MS.gene063628.t1 AT2G32250 26.721 610 373 10 58 639 1 564 2.45e-68 237
MS.gene063628.t1 AT2G32250 26.721 610 373 10 58 639 42 605 3.94e-68 238
MS.gene063628.t1 AT2G32250 26.721 610 373 10 58 639 42 605 3.94e-68 238
MS.gene063628.t1 AT2G32250 26.721 610 373 10 58 639 42 605 3.98e-68 238
MS.gene063628.t1 AT2G32250 26.721 610 373 10 58 639 42 605 3.98e-68 238
MS.gene063628.t1 AT2G32250 26.721 610 373 10 58 639 42 605 3.98e-68 238
MS.gene063628.t1 AT5G18960 29.021 572 345 14 54 600 211 746 1.44e-65 230
MS.gene063628.t1 AT5G18960 29.021 572 345 14 54 600 211 746 1.44e-65 230
MS.gene063628.t1 AT4G38170 32.031 384 249 6 206 582 21 399 1.49e-63 219
MS.gene063628.t1 AT4G19990 27.438 605 349 11 59 615 26 588 5.20e-59 211
MS.gene063628.t1 AT4G19990 27.438 605 349 11 59 615 26 588 5.20e-59 211
MS.gene063628.t1 AT4G19990 27.438 605 349 11 59 615 26 588 5.24e-59 211
MS.gene063628.t1 AT4G19990 27.438 605 349 11 59 615 40 602 5.24e-59 211
MS.gene063628.t1 AT4G19990 27.438 605 349 11 59 615 26 588 6.25e-59 211
MS.gene063628.t1 AT4G19990 27.438 605 349 11 59 615 26 588 6.25e-59 211
MS.gene063628.t1 AT4G19990 27.872 531 333 8 93 615 43 531 7.26e-57 204
MS.gene063628.t1 AT1G52520 27.778 576 345 15 53 580 84 636 8.03e-56 202
MS.gene063628.t1 AT5G28530 25.974 616 397 15 35 602 37 641 1.87e-55 200
MS.gene063628.t1 AT1G80010 27.145 571 370 12 54 582 95 661 3.78e-55 200
MS.gene063628.t1 AT5G28530 26.045 622 385 16 35 602 37 637 5.22e-55 199
MS.gene063628.t1 AT5G28530 26.045 622 385 16 35 602 37 637 5.22e-55 199
MS.gene063628.t1 AT5G28530 23.432 606 362 15 35 602 37 578 5.37e-36 144
MS.gene063628.t1 AT5G28530 23.529 612 350 16 35 602 37 574 1.14e-35 142

Find 104 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAAAGTTTCTTGGCTATT+TGG 0.127787 5.2:-65802145 None:intergenic
GAAATTCTTCATATTCTTCT+TGG 0.203404 5.2:-65803586 None:intergenic
ATAAACGATTATGGATATTT+TGG 0.203454 5.2:+65802819 MS.gene063628:CDS
AGCAAATAACAAATATCTTT+TGG 0.204370 5.2:+65802781 MS.gene063628:CDS
TTGAAATTGATGGCTCATTA+AGG 0.253482 5.2:-65802072 None:intergenic
AGAATTTCCTTCGAACTAAA+AGG 0.257081 5.2:+65802655 MS.gene063628:CDS
AGCAGAAATGGGAGCTCTTT+TGG 0.284940 5.2:-65803157 None:intergenic
GTTATGCCTCAAACATTTCA+TGG 0.308775 5.2:+65803080 MS.gene063628:CDS
GAAGAATATGAAGAATTTCT+AGG 0.312241 5.2:+65803590 MS.gene063628:CDS
TTTGAAGTCAGATATAAATT+TGG 0.313176 5.2:+65803400 MS.gene063628:CDS
GAAAGGACATCATATTGATT+TGG 0.317994 5.2:+65803862 MS.gene063628:CDS
TAGTTCGAAGGAAATTCTTA+TGG 0.319083 5.2:-65802650 None:intergenic
ATAAGGAGAGGAAAGTAATA+TGG 0.322313 5.2:+65803669 MS.gene063628:CDS
TATAATCTACAAAAGTTTCT+TGG 0.325632 5.2:-65802153 None:intergenic
CCTTCAACAATTGAAATTGA+TGG 0.337251 5.2:-65802082 None:intergenic
GCACAGATGATAAACGATTA+TGG 0.341323 5.2:+65802810 MS.gene063628:CDS
TTGTTGAAGGTGTAGAGAAA+TGG 0.358317 5.2:+65802095 MS.gene063628:CDS
AATGAGTATAGCAGGAGAAT+TGG 0.360693 5.2:+65802229 MS.gene063628:CDS
TATAAATGATGATGACAATA+AGG 0.364668 5.2:+65803652 MS.gene063628:CDS
TTTATGTCTTGACTCATAAT+CGG 0.369202 5.2:-65803483 None:intergenic
TGGCAAAATACTTTCATGAA+TGG 0.373730 5.2:-65802125 None:intergenic
CTTCAAAATCCGAGCAGAAA+TGG 0.375608 5.2:-65803169 None:intergenic
ACGTGCTTTACACCCAGTTC+TGG 0.377947 5.2:-65802385 None:intergenic
CATATCCTCAACTATCTTGT+TGG 0.380860 5.2:-65804004 None:intergenic
AAACAATTCCTTCCTTTCAA+TGG 0.386086 5.2:+65802964 MS.gene063628:CDS
TGTTGAAGGTGTAGAGAAAT+GGG 0.392498 5.2:+65802096 MS.gene063628:CDS
GTCTATTCCACCAACTTGCT+TGG 0.399516 5.2:-65802604 None:intergenic
CGACCATTGGGAGAATTTGT+TGG 0.399862 5.2:+65802888 MS.gene063628:CDS
ATAGGTGGTTCACATGCCTT+AGG 0.401174 5.2:-65803133 None:intergenic
TACAACATACAAGACAAATA+AGG 0.410215 5.2:+65802860 MS.gene063628:CDS
AAAATCCTTTGGCTTTAATC+CGG 0.414261 5.2:-65802568 None:intergenic
TGTTGGACTCTCATAGACTT+TGG 0.423482 5.2:-65803987 None:intergenic
TGCATAAAGAATTTGAATGA+TGG 0.425164 5.2:+65803617 MS.gene063628:CDS
ATACTCATTATAAAATTCAT+AGG 0.433466 5.2:-65802215 None:intergenic
TTCAAAATCCGAGCAGAAAT+GGG 0.435563 5.2:-65803168 None:intergenic
ACATACTCATGAAAATCCTT+TGG 0.439521 5.2:-65802579 None:intergenic
TCTAGAGCAGAAACCAGAAC+TGG 0.439558 5.2:+65802372 MS.gene063628:CDS
TTATACACCACACTTGGAAA+TGG 0.441214 5.2:+65802171 MS.gene063628:CDS
TATAGCAGGAGAATTGGATT+TGG 0.445248 5.2:+65802235 MS.gene063628:CDS
ACCACCTCTTTGTCATTCCA+CGG 0.452949 5.2:+65801876 MS.gene063628:CDS
CCATCAATTTCAATTGTTGA+AGG 0.458479 5.2:+65802082 MS.gene063628:CDS
AGGTGGTGGACGGCCAGAAT+TGG 0.466416 5.2:-65801860 None:intergenic
ATGGAGTTTCGAGGATCAAA+AGG 0.467663 5.2:+65803688 MS.gene063628:CDS
AAGATGGAGAAAACCAATTC+TGG 0.469862 5.2:+65801847 None:intergenic
TCAAATAACCATTGAAAGGA+AGG 0.471148 5.2:-65802972 None:intergenic
GGTCAGGAAGTAATCGAGAT+TGG 0.476854 5.2:+65803840 MS.gene063628:CDS
TGAAAGTCATAAAAGAAAGA+AGG 0.477032 5.2:-65802744 None:intergenic
TCCTAAGTTGAAAATTAAGA+AGG 0.489936 5.2:+65803508 MS.gene063628:CDS
CATTTCATGGACTATGCACT+TGG 0.490965 5.2:+65803093 MS.gene063628:CDS
GTTGGTGGAATAGACATAGT+TGG 0.498391 5.2:+65802612 MS.gene063628:CDS
AATAAGGATTATCGACCATT+GGG 0.502322 5.2:+65802876 MS.gene063628:CDS
AAATAAGGATTATCGACCAT+TGG 0.506629 5.2:+65802875 MS.gene063628:CDS
GCATAAAGAATTTGAATGAT+GGG 0.507372 5.2:+65803618 MS.gene063628:CDS
ATGGTCAAATTAACAAATAG+AGG 0.510113 5.2:+65803932 MS.gene063628:CDS
ATAATGTTTCTGATGATTCG+TGG 0.513389 5.2:+65803258 MS.gene063628:CDS
CATAGTCCATGAAATGTTTG+AGG 0.517904 5.2:-65803086 None:intergenic
TGGACAAGAGAAGCAAGGTC+AGG 0.530771 5.2:+65803824 MS.gene063628:CDS
TGTAGATTATACACCACACT+TGG 0.533801 5.2:+65802165 MS.gene063628:CDS
TCACTGAGGTATGGTGAAGT+AGG 0.535157 5.2:+65802684 MS.gene063628:CDS
AAGATGAATTTCTGAACGCA+TGG 0.536411 5.2:+65803216 MS.gene063628:CDS
AACTGGGTGTAAAGCACGTA+TGG 0.543457 5.2:+65802389 MS.gene063628:CDS
ATTGTGTTGGCTGATGAATC+CGG 0.546006 5.2:+65802549 MS.gene063628:CDS
AGGAATCATTCACTGAGGTA+TGG 0.556258 5.2:+65802675 MS.gene063628:CDS
TAGTCATGCTTTGAAAGTGT+TGG 0.559284 5.2:+65803754 MS.gene063628:CDS
TAGGTGGTTCACATGCCTTA+GGG 0.559416 5.2:-65803132 None:intergenic
TCAAAATGCAGCAATGGCCA+AGG 0.565175 5.2:+65803046 MS.gene063628:CDS
GGAAATGGAGTTTGAATCTG+AGG 0.566587 5.2:+65802186 MS.gene063628:CDS
CCACCTCTTTGTCATTCCAC+GGG 0.567560 5.2:+65801877 MS.gene063628:CDS
AACAGATCAAAATGCAGCAA+TGG 0.567887 5.2:+65803040 MS.gene063628:CDS
CACTTCGAAAGAGTTGTGAA+TGG 0.569131 5.2:+65803437 MS.gene063628:CDS
GCATTATTGATGTATTTCAA+AGG 0.569965 5.2:+65802708 MS.gene063628:CDS
TTTGTTAATTTGACCATCCG+AGG 0.571395 5.2:-65803926 None:intergenic
AAAGAGCTCCCATTTCTGCT+CGG 0.571791 5.2:+65803160 MS.gene063628:CDS
GCACGTATGCTTGTTCAAGC+CGG 0.574035 5.2:+65803530 MS.gene063628:CDS
TCAAACTCCATTTCCAAGTG+TGG 0.576119 5.2:-65802178 None:intergenic
GAAAGTAATATGGAGTTTCG+AGG 0.580912 5.2:+65803679 MS.gene063628:CDS
CAATGATTTATGTCCTCGGA+TGG 0.584590 5.2:+65803913 MS.gene063628:CDS
AGTTTCAAATAACCATTGAA+AGG 0.585078 5.2:-65802976 None:intergenic
CGTACCCTGGTGGTTGAAGA+AGG 0.585881 5.2:-65802476 None:intergenic
CGCTTGAGTATATAGTGCGA+AGG 0.588820 5.2:-65803800 None:intergenic
CATAACTAGAGAAATAGCCT+TGG 0.598382 5.2:-65803063 None:intergenic
GTGGAATGACAAAGAGGTGG+TGG 0.601159 5.2:-65801874 None:intergenic
CGCACTATATACTCAAGCGA+TGG 0.601753 5.2:+65803804 MS.gene063628:CDS
AATCACCTTCTTCAACCACC+AGG 0.604960 5.2:+65802471 MS.gene063628:CDS
ACAAGCATCACAAATTGTGT+TGG 0.607468 5.2:+65802536 MS.gene063628:CDS
TAGACATAGTTGGTTATACA+AGG 0.611692 5.2:+65802622 MS.gene063628:CDS
TGTCGTAAATTTGAGACACA+TGG 0.618799 5.2:+65803722 MS.gene063628:CDS
GATACAATGATTTATGTCCT+CGG 0.618907 5.2:+65803909 MS.gene063628:CDS
TATGTGTCCAAGCAAGTTGG+TGG 0.620761 5.2:+65802597 MS.gene063628:CDS
AGTCCAACAAATTCTCCCAA+TGG 0.627219 5.2:-65802891 None:intergenic
GGAAGTAATCGAGATTGGAA+AGG 0.629938 5.2:+65803845 MS.gene063628:CDS
CTAGAGCAGAAACCAGAACT+GGG 0.631153 5.2:+65802373 MS.gene063628:CDS
ATATAAGAGAAAATGCCCTA+AGG 0.636935 5.2:+65803117 MS.gene063628:CDS
CGAATCATATGAACGTACCC+TGG 0.641374 5.2:-65802489 None:intergenic
TGAATCCGGATTAAAGCCAA+AGG 0.646687 5.2:+65802563 MS.gene063628:CDS
AATGACAAAGAGGTGGTGGA+CGG 0.652235 5.2:-65801870 None:intergenic
AGCGATGGACAAGAGAAGCA+AGG 0.654037 5.2:+65803819 MS.gene063628:CDS
ATGATGATGACAATAAGGAG+AGG 0.664429 5.2:+65803657 MS.gene063628:CDS
TTACCCGTGGAATGACAAAG+AGG 0.668554 5.2:-65801880 None:intergenic
CCCGTGGAATGACAAAGAGG+TGG 0.685949 5.2:-65801877 None:intergenic
ATCACCTTCTTCAACCACCA+GGG 0.688985 5.2:+65802472 MS.gene063628:CDS
ATCATATGAACGTACCCTGG+TGG 0.694249 5.2:-65802486 None:intergenic
GAGTATGTGTCCAAGCAAGT+TGG 0.694904 5.2:+65802594 MS.gene063628:CDS
CTAAAAGGAATCATTCACTG+AGG 0.744388 5.2:+65802670 MS.gene063628:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACTCATTATAAAATTCAT+AGG - chr5.2:65802218-65802237 None:intergenic 15.0%
!! TTTGTAGTATTAATTAAAAG+AGG - chr5.2:65801988-65802007 None:intergenic 15.0%
!! TATAATCTACAAAAGTTTCT+TGG - chr5.2:65802156-65802175 None:intergenic 20.0%
!!! AATAAGTATTTTGTTAAACC+AGG - chr5.2:65801930-65801949 None:intergenic 20.0%
!!! AATTTTATAATGAGTATAGC+AGG + chr5.2:65802221-65802240 MS.gene063628:CDS 20.0%
!!! AGCAAATAACAAATATCTTT+TGG + chr5.2:65802781-65802800 MS.gene063628:CDS 20.0%
!!! ATAAACGATTATGGATATTT+TGG + chr5.2:65802819-65802838 MS.gene063628:CDS 20.0%
!!! GCAAATAACAAATATCTTTT+GGG + chr5.2:65802782-65802801 MS.gene063628:CDS 20.0%
!!! TATAAATGATGATGACAATA+AGG + chr5.2:65803652-65803671 MS.gene063628:CDS 20.0%
!!! TTTCTTTTATGACTTTCAAA+TGG + chr5.2:65802749-65802768 MS.gene063628:CDS 20.0%
!!! TTTGAAGTCAGATATAAATT+TGG + chr5.2:65803400-65803419 MS.gene063628:CDS 20.0%
! AGTTTCAAATAACCATTGAA+AGG - chr5.2:65802979-65802998 None:intergenic 25.0%
! ATGGTCAAATTAACAAATAG+AGG + chr5.2:65803932-65803951 MS.gene063628:CDS 25.0%
! ATTTCACTTGATCGAAAAAT+TGG + chr5.2:65802414-65802433 MS.gene063628:CDS 25.0%
! GAAATTCTTCATATTCTTCT+TGG - chr5.2:65803589-65803608 None:intergenic 25.0%
! GAAGAATATGAAGAATTTCT+AGG + chr5.2:65803590-65803609 MS.gene063628:CDS 25.0%
! GCATAAAGAATTTGAATGAT+GGG + chr5.2:65803618-65803637 MS.gene063628:CDS 25.0%
! GCATTATTGATGTATTTCAA+AGG + chr5.2:65802708-65802727 MS.gene063628:CDS 25.0%
! GTAGTATTAATTAAAAGAGG+AGG - chr5.2:65801985-65802004 None:intergenic 25.0%
! TACAACATACAAGACAAATA+AGG + chr5.2:65802860-65802879 MS.gene063628:CDS 25.0%
! TCGAAAAATTGGAAAATACA+AGG + chr5.2:65802425-65802444 MS.gene063628:CDS 25.0%
! TGAAAGTCATAAAAGAAAGA+AGG - chr5.2:65802747-65802766 None:intergenic 25.0%
! TGCATAAAGAATTTGAATGA+TGG + chr5.2:65803617-65803636 MS.gene063628:CDS 25.0%
! TTACACTCCAAAAATATTTG+AGG + chr5.2:65803559-65803578 MS.gene063628:CDS 25.0%
! TTTATGTCTTGACTCATAAT+CGG - chr5.2:65803486-65803505 None:intergenic 25.0%
!! AATAACAAATATCTTTTGGG+CGG + chr5.2:65802785-65802804 MS.gene063628:CDS 25.0%
!! TCCTAAGTTGAAAATTAAGA+AGG + chr5.2:65803508-65803527 MS.gene063628:CDS 25.0%
AAACAATTCCTTCCTTTCAA+TGG + chr5.2:65802964-65802983 MS.gene063628:CDS 30.0%
AAATAAGGATTATCGACCAT+TGG + chr5.2:65802875-65802894 MS.gene063628:CDS 30.0%
AATAAGGATTATCGACCATT+GGG + chr5.2:65802876-65802895 MS.gene063628:CDS 30.0%
ACAAAAGTTTCTTGGCTATT+TGG - chr5.2:65802148-65802167 None:intergenic 30.0%
ACATACTCATGAAAATCCTT+TGG - chr5.2:65802582-65802601 None:intergenic 30.0%
AGAATTTCCTTCGAACTAAA+AGG + chr5.2:65802655-65802674 MS.gene063628:CDS 30.0%
ATAAGGAGAGGAAAGTAATA+TGG + chr5.2:65803669-65803688 MS.gene063628:CDS 30.0%
ATATAAGAGAAAATGCCCTA+AGG + chr5.2:65803117-65803136 MS.gene063628:CDS 30.0%
CACTCATTATTATTGAAACC+TGG + chr5.2:65801909-65801928 MS.gene063628:intron 30.0%
CCATCAATTTCAATTGTTGA+AGG + chr5.2:65802082-65802101 MS.gene063628:CDS 30.0%
CCTTCAACAATTGAAATTGA+TGG - chr5.2:65802085-65802104 None:intergenic 30.0%
GATACAATGATTTATGTCCT+CGG + chr5.2:65803909-65803928 MS.gene063628:CDS 30.0%
GGCAATAAAAGCAAAAAAGA+CGG + chr5.2:65802274-65802293 MS.gene063628:intron 30.0%
TAGACATAGTTGGTTATACA+AGG + chr5.2:65802622-65802641 MS.gene063628:CDS 30.0%
TAGTTCGAAGGAAATTCTTA+TGG - chr5.2:65802653-65802672 None:intergenic 30.0%
TCAAATAACCATTGAAAGGA+AGG - chr5.2:65802975-65802994 None:intergenic 30.0%
TGGCAAAATACTTTCATGAA+TGG - chr5.2:65802128-65802147 None:intergenic 30.0%
TGGGAAAAATATTGAGTCAA+TGG - chr5.2:65802047-65802066 None:intergenic 30.0%
TTTCGAAGTGTGTGAAAAAT+TGG - chr5.2:65803427-65803446 None:intergenic 30.0%
! ATAATGTTTCTGATGATTCG+TGG + chr5.2:65803258-65803277 MS.gene063628:CDS 30.0%
! GAAACTTTTCTCAAAGCAAT+GGG + chr5.2:65802993-65803012 MS.gene063628:CDS 30.0%
! GAAAGGACATCATATTGATT+TGG + chr5.2:65803862-65803881 MS.gene063628:CDS 30.0%
! GCCTTCTTAATTTTCAACTT+AGG - chr5.2:65803512-65803531 None:intergenic 30.0%
! TGAAACTTTTCTCAAAGCAA+TGG + chr5.2:65802992-65803011 MS.gene063628:CDS 30.0%
! TGGAACTCCTCAAATATTTT+TGG - chr5.2:65803569-65803588 None:intergenic 30.0%
! TTGAAATTGATGGCTCATTA+AGG - chr5.2:65802075-65802094 None:intergenic 30.0%
!! AAAATCCTTTGGCTTTAATC+CGG - chr5.2:65802571-65802590 None:intergenic 30.0%
!! ATTGACTCAATATTTTTCCC+AGG + chr5.2:65802046-65802065 MS.gene063628:intron 30.0%
!!! AATGATTCCTTTTAGTTCGA+AGG - chr5.2:65802665-65802684 None:intergenic 30.0%
!!! TCAGACAAACTGTTGTTTTT+GGG + chr5.2:65802922-65802941 MS.gene063628:CDS 30.0%
!!! TTCAGACAAACTGTTGTTTT+TGG + chr5.2:65802921-65802940 MS.gene063628:CDS 30.0%
!!! TTTTCGAGTGAAGGAAAAAT+GGG + chr5.2:65803292-65803311 MS.gene063628:CDS 30.0%
!!! TTTTGGAGTGTAAATGTTTC+CGG - chr5.2:65803552-65803571 None:intergenic 30.0%
!!! TTTTTCGAGTGAAGGAAAAA+TGG + chr5.2:65803291-65803310 MS.gene063628:CDS 30.0%
AACAGATCAAAATGCAGCAA+TGG + chr5.2:65803040-65803059 MS.gene063628:CDS 35.0%
AAGATGAATTTCTGAACGCA+TGG + chr5.2:65803216-65803235 MS.gene063628:CDS 35.0%
AATGAGTATAGCAGGAGAAT+TGG + chr5.2:65802229-65802248 MS.gene063628:CDS 35.0%
ACAAGCATCACAAATTGTGT+TGG + chr5.2:65802536-65802555 MS.gene063628:CDS 35.0%
CATAACTAGAGAAATAGCCT+TGG - chr5.2:65803066-65803085 None:intergenic 35.0%
CATAGTCCATGAAATGTTTG+AGG - chr5.2:65803089-65803108 None:intergenic 35.0%
CTAAAAGGAATCATTCACTG+AGG + chr5.2:65802670-65802689 MS.gene063628:CDS 35.0%
GAAAGTAATATGGAGTTTCG+AGG + chr5.2:65803679-65803698 MS.gene063628:CDS 35.0%
GCACAGATGATAAACGATTA+TGG + chr5.2:65802810-65802829 MS.gene063628:CDS 35.0%
GTTATGCCTCAAACATTTCA+TGG + chr5.2:65803080-65803099 MS.gene063628:CDS 35.0%
TATAGCAGGAGAATTGGATT+TGG + chr5.2:65802235-65802254 MS.gene063628:CDS 35.0%
TGTAGATTATACACCACACT+TGG + chr5.2:65802165-65802184 MS.gene063628:CDS 35.0%
TGTTCGAAAAACTTTCACTG+CGG + chr5.2:65803325-65803344 MS.gene063628:CDS 35.0%
TTATACACCACACTTGGAAA+TGG + chr5.2:65802171-65802190 MS.gene063628:CDS 35.0%
TTCAAAATCCGAGCAGAAAT+GGG - chr5.2:65803171-65803190 None:intergenic 35.0%
TTTGGTATTCGTCGAGAATA+TGG + chr5.2:65802253-65802272 MS.gene063628:intron 35.0%
! ATGATGATGACAATAAGGAG+AGG + chr5.2:65803657-65803676 MS.gene063628:CDS 35.0%
! TGATCTGTTAGGATGGTTTT+TGG - chr5.2:65803029-65803048 None:intergenic 35.0%
! TGTCGTAAATTTGAGACACA+TGG + chr5.2:65803722-65803741 MS.gene063628:CDS 35.0%
! TGTTGAAGGTGTAGAGAAAT+GGG + chr5.2:65802096-65802115 MS.gene063628:CDS 35.0%
! TTGTTGAAGGTGTAGAGAAA+TGG + chr5.2:65802095-65802114 MS.gene063628:CDS 35.0%
! TTTGTTAATTTGACCATCCG+AGG - chr5.2:65803929-65803948 None:intergenic 35.0%
!! AAGATTCTCGTGCTTTAAAG+AGG + chr5.2:65802312-65802331 MS.gene063628:intron 35.0%
!! AGATTCTCGTGCTTTAAAGA+GGG + chr5.2:65802313-65802332 MS.gene063628:intron 35.0%
!! TAGTCATGCTTTGAAAGTGT+TGG + chr5.2:65803754-65803773 MS.gene063628:CDS 35.0%
!! TGCATTTTGATCTGTTAGGA+TGG - chr5.2:65803036-65803055 None:intergenic 35.0%
!! TTGCTGCATTTTGATCTGTT+AGG - chr5.2:65803040-65803059 None:intergenic 35.0%
!!! CAGACAAACTGTTGTTTTTG+GGG + chr5.2:65802923-65802942 MS.gene063628:CDS 35.0%
!!! GCATTCAATTTTTCGAGTGA+AGG + chr5.2:65803283-65803302 MS.gene063628:CDS 35.0%
AGGAATCATTCACTGAGGTA+TGG + chr5.2:65802675-65802694 MS.gene063628:CDS 40.0%
AGTCCAACAAATTCTCCCAA+TGG - chr5.2:65802894-65802913 None:intergenic 40.0%
AGTGAAGGAAAAATGGGCAT+GGG + chr5.2:65803298-65803317 MS.gene063628:CDS 40.0%
ATGAGTGAATTTCTTACCCG+TGG - chr5.2:65801896-65801915 None:intergenic 40.0%
ATGGAGTTTCGAGGATCAAA+AGG + chr5.2:65803688-65803707 MS.gene063628:CDS 40.0%
ATGGCTCATTAAGGTTTCCT+GGG - chr5.2:65802066-65802085 None:intergenic 40.0%
CAATGATTTATGTCCTCGGA+TGG + chr5.2:65803913-65803932 MS.gene063628:CDS 40.0%
CACTTCGAAAGAGTTGTGAA+TGG + chr5.2:65803437-65803456 MS.gene063628:CDS 40.0%
CTTCAAAATCCGAGCAGAAA+TGG - chr5.2:65803172-65803191 None:intergenic 40.0%
GGAAATGGAGTTTGAATCTG+AGG + chr5.2:65802186-65802205 MS.gene063628:CDS 40.0%
GGAAGTAATCGAGATTGGAA+AGG + chr5.2:65803845-65803864 MS.gene063628:CDS 40.0%
GTTCGAAAAACTTTCACTGC+GGG + chr5.2:65803326-65803345 MS.gene063628:CDS 40.0%
TCAAACTCCATTTCCAAGTG+TGG - chr5.2:65802181-65802200 None:intergenic 40.0%
TGAATCCGGATTAAAGCCAA+AGG + chr5.2:65802563-65802582 MS.gene063628:CDS 40.0%
TGTTGGACTCTCATAGACTT+TGG - chr5.2:65803990-65804009 None:intergenic 40.0%
! AATGGGAGCTCTTTTGGTAT+AGG - chr5.2:65803154-65803173 None:intergenic 40.0%
! ACTTTTCTCAAAGCAATGGG+TGG + chr5.2:65802996-65803015 MS.gene063628:CDS 40.0%
! CATTTCATGGACTATGCACT+TGG + chr5.2:65803093-65803112 MS.gene063628:CDS 40.0%
! CTCTAGACTCTTTTGTCAAG+TGG - chr5.2:65802359-65802378 None:intergenic 40.0%
! GTTGGTGGAATAGACATAGT+TGG + chr5.2:65802612-65802631 MS.gene063628:CDS 40.0%
!! ATTGTGTTGGCTGATGAATC+CGG + chr5.2:65802549-65802568 MS.gene063628:CDS 40.0%
AAAGAGCTCCCATTTCTGCT+CGG + chr5.2:65803160-65803179 MS.gene063628:CDS 45.0%
AACTGGGTGTAAAGCACGTA+TGG + chr5.2:65802389-65802408 MS.gene063628:CDS 45.0%
AATCACCTTCTTCAACCACC+AGG + chr5.2:65802471-65802490 MS.gene063628:CDS 45.0%
ACCACCTCTTTGTCATTCCA+CGG + chr5.2:65801876-65801895 MS.gene063628:CDS 45.0%
ATAGGTGGTTCACATGCCTT+AGG - chr5.2:65803136-65803155 None:intergenic 45.0%
ATCACCTTCTTCAACCACCA+GGG + chr5.2:65802472-65802491 MS.gene063628:CDS 45.0%
ATCATATGAACGTACCCTGG+TGG - chr5.2:65802489-65802508 None:intergenic 45.0%
CGAATCATATGAACGTACCC+TGG - chr5.2:65802492-65802511 None:intergenic 45.0%
CGACCATTGGGAGAATTTGT+TGG + chr5.2:65802888-65802907 MS.gene063628:CDS 45.0%
CGCACTATATACTCAAGCGA+TGG + chr5.2:65803804-65803823 MS.gene063628:CDS 45.0%
CGCTTGAGTATATAGTGCGA+AGG - chr5.2:65803803-65803822 None:intergenic 45.0%
CTAGAGCAGAAACCAGAACT+GGG + chr5.2:65802373-65802392 MS.gene063628:CDS 45.0%
GAGTATGTGTCCAAGCAAGT+TGG + chr5.2:65802594-65802613 MS.gene063628:CDS 45.0%
GAGTGAAGGAAAAATGGGCA+TGG + chr5.2:65803297-65803316 MS.gene063628:CDS 45.0%
GATGGCTCATTAAGGTTTCC+TGG - chr5.2:65802067-65802086 None:intergenic 45.0%
GGTCAGGAAGTAATCGAGAT+TGG + chr5.2:65803840-65803859 MS.gene063628:CDS 45.0%
TAGGTGGTTCACATGCCTTA+GGG - chr5.2:65803135-65803154 None:intergenic 45.0%
TCAAAATGCAGCAATGGCCA+AGG + chr5.2:65803046-65803065 MS.gene063628:CDS 45.0%
TCACTGAGGTATGGTGAAGT+AGG + chr5.2:65802684-65802703 MS.gene063628:CDS 45.0%
TTACCCGTGGAATGACAAAG+AGG - chr5.2:65801883-65801902 None:intergenic 45.0%
! AGCAGAAATGGGAGCTCTTT+TGG - chr5.2:65803160-65803179 None:intergenic 45.0%
! GTCTATTCCACCAACTTGCT+TGG - chr5.2:65802607-65802626 None:intergenic 45.0%
! TATGTGTCCAAGCAAGTTGG+TGG + chr5.2:65802597-65802616 MS.gene063628:CDS 45.0%
! TCTAGAGCAGAAACCAGAAC+TGG + chr5.2:65802372-65802391 MS.gene063628:CDS 45.0%
!! AATGACAAAGAGGTGGTGGA+CGG - chr5.2:65801873-65801892 None:intergenic 45.0%
!! CTCGTGCTTTAAAGAGGGAA+AGG + chr5.2:65802318-65802337 MS.gene063628:intron 45.0%
ACGTGCTTTACACCCAGTTC+TGG - chr5.2:65802388-65802407 None:intergenic 50.0%
AGCGATGGACAAGAGAAGCA+AGG + chr5.2:65803819-65803838 MS.gene063628:CDS 50.0%
CCACCTCTTTGTCATTCCAC+GGG + chr5.2:65801877-65801896 MS.gene063628:CDS 50.0%
GCACGTATGCTTGTTCAAGC+CGG + chr5.2:65803530-65803549 MS.gene063628:CDS 50.0%
GTGGAATGACAAAGAGGTGG+TGG - chr5.2:65801877-65801896 None:intergenic 50.0%
TGGACAAGAGAAGCAAGGTC+AGG + chr5.2:65803824-65803843 MS.gene063628:CDS 50.0%
! GGGAGCTCTTTTGGTATAGG+TGG - chr5.2:65803151-65803170 None:intergenic 50.0%
CCCGTGGAATGACAAAGAGG+TGG - chr5.2:65801880-65801899 None:intergenic 55.0%
CGTACCCTGGTGGTTGAAGA+AGG - chr5.2:65802479-65802498 None:intergenic 55.0%
!! AGGTGGTGGACGGCCAGAAT+TGG - chr5.2:65801863-65801882 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 65801850 65804015 65801850 ID=MS.gene063628
chr5.2 mRNA 65801850 65804015 65801850 ID=MS.gene063628.t1;Parent=MS.gene063628
chr5.2 exon 65801850 65801898 65801850 ID=MS.gene063628.t1.exon1;Parent=MS.gene063628.t1
chr5.2 CDS 65801850 65801898 65801850 ID=cds.MS.gene063628.t1;Parent=MS.gene063628.t1
chr5.2 exon 65802068 65802256 65802068 ID=MS.gene063628.t1.exon2;Parent=MS.gene063628.t1
chr5.2 CDS 65802068 65802256 65802068 ID=cds.MS.gene063628.t1;Parent=MS.gene063628.t1
chr5.2 exon 65802334 65804015 65802334 ID=MS.gene063628.t1.exon3;Parent=MS.gene063628.t1
chr5.2 CDS 65802334 65804015 65802334 ID=cds.MS.gene063628.t1;Parent=MS.gene063628.t1
Gene Sequence

>MS.gene063628

ATGGAGAAAACCAATTCTGGCCGTCCACCACCTCTTTGTCATTCCACGGGTAAGAAATTCACTCATTATTATTGAAACCTGGTTTAACAAAATACTTATTTCTCATTCTTTGATCACGTTTCTTGAAAAAATCCTCCTCTTTTAATTAATACTACAAAATTGCATTTGATTTTACTTGTTGTTTTTTTCGTTAACCATTGACTCAATATTTTTCCCAGGAAACCTTAATGAGCCATCAATTTCAATTGTTGAAGGTGTAGAGAAATGGGTTAGTTCCATTCATGAAAGTATTTTGCCAAATAGCCAAGAAACTTTTGTAGATTATACACCACACTTGGAAATGGAGTTTGAATCTGAGGCAGCAGCCTATGAATTTTATAATGAGTATAGCAGGAGAATTGGATTTGGTATTCGTCGAGAATATGGCAATAAAAGCAAAAAAGACGGAATTCTGACTTCAAGAAGATTCTCGTGCTTTAAAGAGGGAAAGGTGATGACAAGCGAGACCACTTGACAAAAGAGTCTAGAGCAGAAACCAGAACTGGGTGTAAAGCACGTATGGTTATTTCACTTGATCGAAAAATTGGAAAATACAAGGTTGTCGACTTTGTATTTGAACATAATCACCTTCTTCAACCACCAGGGTACGTTCATATGATTCGCTCTCATCGACGCATATCTGAGTCACAAGCATCACAAATTGTGTTGGCTGATGAATCCGGATTAAAGCCAAAGGATTTTCATGAGTATGTGTCCAAGCAAGTTGGTGGAATAGACATAGTTGGTTATACAAGGCAAGACCATAAGAATTTCCTTCGAACTAAAAGGAATCATTCACTGAGGTATGGTGAAGTAGGTGCATTATTGATGTATTTCAAAGGACAAAGTGAAAATCCTTCTTTCTTTTATGACTTTCAAATGGATGTTGAAGAGCAAATAACAAATATCTTTTGGGCGGATGCACAGATGATAAACGATTATGGATATTTTGGTGATGTCATCACTTTTGATACAACATACAAGACAAATAAGGATTATCGACCATTGGGAGAATTTGTTGGACTGAATAACTTCAGACAAACTGTTGTTTTTGGGGCAACATTGTTGTATGATGAAACAATTCCTTCCTTTCAATGGTTATTTGAAACTTTTCTCAAAGCAATGGGTGGAGAAAAGCCAAAAACCATCCTAACAGATCAAAATGCAGCAATGGCCAAGGCTATTTCTCTAGTTATGCCTCAAACATTTCATGGACTATGCACTTGGCATATAAGAGAAAATGCCCTAAGGCATGTGAACCACCTATACCAAAAGAGCTCCCATTTCTGCTCGGATTTTGAAGCATGTATTGATCTACATGAAGAAGAAGATGAATTTCTGAACGCATGGAATGCTTTACTTCTTGAGCATAATGTTTCTGATGATTCGTGGCTGCATTCAATTTTTCGAGTGAAGGAAAAATGGGCATGGGCTTATGTTCGAAAAACTTTCACTGCGGGTATGAGATCTACTCAATTAAGTGAGAGCTTCAATGCTGCTTTGAAAAATCATTTGAAGTCAGATATAAATTTGGTCCAATTTTTCACACACTTCGAAAGAGTTGTGAATGGAAAACGAAACAATGAGTCAGAAGCCGATTATGAGTCAAGACATAAACTTCCTAAGTTGAAAATTAAGAAGGCACGTATGCTTGTTCAAGCCGGAAACATTTACACTCCAAAAATATTTGAGGAGTTCCAAGAAGAATATGAAGAATTTCTAGGCACTTGCATAAAGAATTTGAATGATGGGTTGTATATTGTTATAAATGATGATGACAATAAGGAGAGGAAAGTAATATGGAGTTTCGAGGATCAAAAGGTTGCTTGTGATTGTCGTAAATTTGAGACACATGGTATTTTATGTAGTCATGCTTTGAAAGTGTTGGATACAATGAATATTAAGCTAATTCCTTCGCACTATATACTCAAGCGATGGACAAGAGAAGCAAGGTCAGGAAGTAATCGAGATTGGAAAGGACATCATATTGATTTGGACATCAAAGCTCACTTCATGAAGCGATACAATGATTTATGTCCTCGGATGGTCAAATTAACAAATAGAGGATCTGAAACTCACGAAACTTACATGTTTCTGTCCAAAGTCTATGAGAGTCCAACAAGATAG
Protein sequence

>MS.gene063628.t1

MEKTNSGRPPPLCHSTGNLNEPSISIVEGVEKWVSSIHESILPNSQETFVDYTPHLEMEFESEAAAYEFYNEYSRRIGFGKGDDKRDHLTKESRAETRTGCKARMVISLDRKIGKYKVVDFVFEHNHLLQPPGYVHMIRSHRRISESQASQIVLADESGLKPKDFHEYVSKQVGGIDIVGYTRQDHKNFLRTKRNHSLRYGEVGALLMYFKGQSENPSFFYDFQMDVEEQITNIFWADAQMINDYGYFGDVITFDTTYKTNKDYRPLGEFVGLNNFRQTVVFGATLLYDETIPSFQWLFETFLKAMGGEKPKTILTDQNAAMAKAISLVMPQTFHGLCTWHIRENALRHVNHLYQKSSHFCSDFEACIDLHEEEDEFLNAWNALLLEHNVSDDSWLHSIFRVKEKWAWAYVRKTFTAGMRSTQLSESFNAALKNHLKSDINLVQFFTHFERVVNGKRNNESEADYESRHKLPKLKIKKARMLVQAGNIYTPKIFEEFQEEYEEFLGTCIKNLNDGLYIVINDDDNKERKVIWSFEDQKVACDCRKFETHGILCSHALKVLDTMNIKLIPSHYILKRWTREARSGSNRDWKGHHIDLDIKAHFMKRYNDLCPRMVKLTNRGSETHETYMFLSKVYESPTR