AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063953

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063953.t1 MTR_8g043960 92.857 322 14 1 1 322 1 313 0.0 593
MS.gene063953.t1 MTR_3g010660 64.396 323 108 4 6 322 1 322 1.70e-143 407
MS.gene063953.t1 MTR_7g088090 47.006 334 146 8 6 322 1 320 1.62e-85 260
MS.gene063953.t1 MTR_6g026800 49.398 332 142 7 6 322 1 321 4.36e-82 251
MS.gene063953.t1 MTR_4g085910 34.604 341 154 13 23 301 17 350 9.77e-37 135
MS.gene063953.t1 MTR_5g088230 54.967 151 61 3 1 148 1 147 6.62e-35 128
MS.gene063953.t1 MTR_5g088230 61.364 88 33 1 235 322 183 269 1.66e-24 100
MS.gene063953.t1 MTR_7g104480 31.624 351 147 9 30 294 45 388 2.25e-33 127
MS.gene063953.t1 MTR_5g088230 54.110 146 60 3 6 148 1 142 7.24e-32 120
MS.gene063953.t1 MTR_5g088230 61.364 88 33 1 235 322 178 264 1.51e-24 100
MS.gene063953.t1 MTR_3g101780 48.529 136 62 3 12 144 14 144 1.64e-29 117
MS.gene063953.t1 MTR_3g101780 63.768 69 22 1 229 297 333 398 3.52e-17 82.0
MS.gene063953.t1 MTR_2g086390 34.737 285 127 12 27 294 11 253 2.82e-29 114
MS.gene063953.t1 MTR_2g086390 34.737 285 127 12 27 294 11 253 3.51e-29 113
MS.gene063953.t1 MTR_2g086340 35.563 284 145 10 26 294 24 284 1.92e-28 112
MS.gene063953.t1 MTR_5g060990 31.561 301 157 11 16 273 4 298 4.10e-24 100
MS.gene063953.t1 MTR_4g094390 53.521 71 29 1 238 304 117 187 2.36e-12 65.5
MS.gene063953.t1 MTR_8g075130 44.828 87 36 3 243 322 97 178 8.42e-12 63.5
MS.gene063953.t1 MTR_7g015990 70.000 50 12 1 251 297 120 169 1.75e-11 62.8
MS.gene063953.t1 MTR_5g022780 61.017 59 11 1 251 297 166 224 3.08e-11 63.2
MS.gene063953.t1 MTR_1g098590 62.745 51 19 0 246 296 157 207 6.82e-11 62.0
MS.gene063953.t1 MTR_1g098590 62.745 51 19 0 246 296 157 207 7.51e-11 61.6
MS.gene063953.t1 MTR_1g098590 62.745 51 19 0 246 296 157 207 9.84e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063953.t1 AT3G56850 53.055 311 107 8 23 322 15 297 3.90e-89 269
MS.gene063953.t1 AT2G41070 49.338 302 93 10 23 320 15 260 8.05e-74 228
MS.gene063953.t1 AT2G41070 49.338 302 93 10 23 320 15 260 8.05e-74 228
MS.gene063953.t1 AT2G41070 49.338 302 93 10 23 320 15 260 8.05e-74 228
MS.gene063953.t1 AT2G41070 49.338 302 93 10 23 320 24 269 1.06e-73 228
MS.gene063953.t1 AT2G41070 49.640 278 80 10 23 296 15 236 4.19e-65 206
MS.gene063953.t1 AT2G36270 33.092 414 156 15 23 322 36 442 2.68e-37 138
MS.gene063953.t1 AT2G36270 33.092 414 156 15 23 322 36 442 2.68e-37 138
MS.gene063953.t1 AT2G36270 33.508 382 140 12 23 297 36 410 4.62e-36 135
MS.gene063953.t1 AT3G44460 38.947 285 128 12 26 294 37 291 4.20e-29 114
MS.gene063953.t1 AT3G19290 46.429 140 56 3 24 144 34 173 1.57e-25 105
MS.gene063953.t1 AT3G19290 46.429 140 56 3 24 144 34 173 2.00e-25 105
MS.gene063953.t1 AT3G19290 59.016 61 22 1 236 296 340 397 1.40e-13 71.2
MS.gene063953.t1 AT3G19290 46.429 140 56 3 24 144 34 173 2.37e-25 105
MS.gene063953.t1 AT3G19290 59.016 61 22 1 236 296 340 397 2.17e-13 70.9
MS.gene063953.t1 AT3G19290 46.429 140 56 3 24 144 34 173 2.37e-25 105
MS.gene063953.t1 AT3G19290 59.016 61 22 1 236 296 340 397 2.17e-13 70.9
MS.gene063953.t1 AT3G19290 46.429 140 56 3 24 144 34 173 2.39e-25 105
MS.gene063953.t1 AT3G19290 59.016 61 22 1 236 296 340 397 2.13e-13 70.9
MS.gene063953.t1 AT4G34000 39.130 161 58 3 24 144 27 187 8.75e-24 101
MS.gene063953.t1 AT4G34000 41.447 152 74 6 177 317 293 440 2.77e-16 79.7
MS.gene063953.t1 AT4G34000 39.130 161 58 3 24 144 27 187 1.17e-23 101
MS.gene063953.t1 AT4G34000 44.444 135 60 6 177 301 293 422 3.97e-16 79.3
MS.gene063953.t1 AT4G34000 39.130 161 58 3 24 144 27 187 1.17e-23 101
MS.gene063953.t1 AT4G34000 44.444 135 60 6 177 301 293 422 3.97e-16 79.3
MS.gene063953.t1 AT4G34000 39.130 161 58 3 24 144 27 187 1.17e-23 101
MS.gene063953.t1 AT4G34000 44.444 135 60 6 177 301 293 422 3.97e-16 79.3
MS.gene063953.t1 AT5G42910 28.763 372 167 16 23 319 19 367 5.91e-22 95.5
MS.gene063953.t1 AT1G45249 41.176 136 62 3 24 144 21 153 2.87e-21 93.6
MS.gene063953.t1 AT1G45249 41.176 136 62 3 24 144 21 153 2.87e-21 93.6
MS.gene063953.t1 AT1G45249 34.392 189 91 5 24 190 36 213 5.09e-21 93.2
MS.gene063953.t1 AT1G45249 52.632 95 33 2 236 322 341 431 1.18e-17 83.6
MS.gene063953.t1 AT1G45249 34.392 189 91 5 24 190 36 213 5.09e-21 93.2
MS.gene063953.t1 AT1G45249 52.632 95 33 2 236 322 341 431 1.18e-17 83.6
MS.gene063953.t1 AT1G45249 41.176 136 62 3 24 144 21 153 6.70e-21 92.8
MS.gene063953.t1 AT1G45249 41.176 136 62 3 24 144 21 153 6.70e-21 92.8
MS.gene063953.t1 AT1G45249 41.176 136 62 3 24 144 21 153 6.70e-21 92.8
MS.gene063953.t1 AT1G45249 41.176 136 62 3 24 144 21 153 6.70e-21 92.8
MS.gene063953.t1 AT1G45249 34.392 189 91 5 24 190 21 198 6.90e-21 92.8
MS.gene063953.t1 AT1G45249 52.632 95 33 2 236 322 326 416 9.11e-18 84.0
MS.gene063953.t1 AT1G45249 34.392 189 91 5 24 190 21 198 9.36e-21 92.4
MS.gene063953.t1 AT1G45249 47.170 106 33 3 236 322 326 427 6.46e-15 75.5
MS.gene063953.t1 AT5G44080 33.476 233 104 9 100 320 120 313 1.70e-18 84.7
MS.gene063953.t1 AT4G35900 51.136 88 38 3 236 322 202 285 6.06e-17 80.1
MS.gene063953.t1 AT1G49720 46.875 96 39 2 236 322 300 392 4.47e-14 72.8
MS.gene063953.t1 AT1G49720 46.875 96 39 2 236 322 300 392 4.47e-14 72.8
MS.gene063953.t1 AT2G17770 39.850 133 72 4 193 322 68 195 5.62e-14 70.1
MS.gene063953.t1 AT1G49720 62.295 61 20 1 236 296 300 357 3.50e-13 70.1
MS.gene063953.t1 AT1G03970 29.258 229 88 7 100 310 85 257 3.69e-12 66.2

Find 82 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCATTGGGTGCACCTCTTA+TGG 0.223647 8.1:-60802453 MS.gene063953:CDS
ATTCGACGTAGCTGATATTT+AGG 0.229724 8.1:+60801251 None:intergenic
GATGACATTGGAGGATTTCT+TGG 0.249290 8.1:-60802723 MS.gene063953:CDS
CAAAATCAATTAGGTGACTT+AGG 0.335660 8.1:-60802995 MS.gene063953:CDS
CGAGAGAGACAAACTACTTT+AGG 0.337274 8.1:-60802749 MS.gene063953:CDS
CGCAGATGCCTGGAAGAAAA+AGG 0.341085 8.1:-60802415 MS.gene063953:CDS
GATGAAGTTCAAAATCAATT+AGG 0.351054 8.1:-60803004 MS.gene063953:CDS
AAGGAGCACTCCAGAGGATA+TGG 0.352037 8.1:-60802396 MS.gene063953:CDS
CTGTTTCCGTGCTCTTGAAC+GGG 0.379424 8.1:+60802316 None:intergenic
GCCTTCATTGTCCGTATTCG+TGG 0.383060 8.1:+60802904 None:intergenic
CCGTTCAAGAGCACGGAAAC+AGG 0.394263 8.1:-60802315 MS.gene063953:intron
TCTACAACGTCAAGCTAGTC+TGG 0.406297 8.1:-60802858 MS.gene063953:CDS
GCATTGGGTGCACCTCTTAT+GGG 0.408513 8.1:-60802452 MS.gene063953:CDS
GCAGGCTTACACAAATGAAT+TGG 0.412384 8.1:-60802190 MS.gene063953:intron
CATTGTCCGTATTCGTGGAC+TGG 0.421785 8.1:+60802909 None:intergenic
AGAGGACTGTGGATGAGGTT+TGG 0.422682 8.1:-60802814 MS.gene063953:CDS
TCTAGGTTCATACTGCTTAG+TGG 0.432596 8.1:+60802968 None:intergenic
TGATATTTAGGTTCAGCAAT+TGG 0.433568 8.1:+60801263 None:intergenic
AAAATCAATTAGGTGACTTA+GGG 0.436185 8.1:-60802994 MS.gene063953:CDS
AGTATCACAGTTTCCTCTGC+AGG 0.436415 8.1:-60802621 MS.gene063953:CDS
TCCGACACAGTTCCCATAAG+AGG 0.453901 8.1:+60802440 None:intergenic
TCCGTTTGCAGATAGTCATA+TGG 0.455759 8.1:-60802474 MS.gene063953:CDS
AGAAAAGGATGATCAAGAAT+CGG 0.457820 8.1:-60802349 MS.gene063953:CDS
GCAGATAGTCATATGGCATT+GGG 0.461753 8.1:-60802467 MS.gene063953:CDS
CTGTTGAGAGAAGGCAGAAA+AGG 0.461801 8.1:-60802364 MS.gene063953:CDS
TGCAGATAGTCATATGGCAT+TGG 0.465956 8.1:-60802468 MS.gene063953:CDS
CCTGTTTCCGTGCTCTTGAA+CGG 0.468292 8.1:+60802315 None:intergenic
GTGTCGGATACGCAGATGCC+TGG 0.469471 8.1:-60802425 MS.gene063953:CDS
GGTTCAGCAATTGGTGTACA+TGG 0.473186 8.1:+60801272 None:intergenic
CAGCAAGAGGACTGTGGATG+AGG 0.475392 8.1:-60802819 MS.gene063953:CDS
TAAATGGGAACTCAGACAAT+GGG 0.478433 8.1:-60803112 MS.gene063953:CDS
GAGGATTTCTTGGTGAAAGC+AGG 0.483426 8.1:-60802713 MS.gene063953:CDS
GGGTCTCAAGGTGGTGGAGA+TGG 0.487387 8.1:-60803091 MS.gene063953:CDS
GTCTTCTCCACCATATCCTC+TGG 0.490528 8.1:+60802386 None:intergenic
ATAAATGGGAACTCAGACAA+TGG 0.492628 8.1:-60803113 None:intergenic
TGATACTGCCACATTTGAAT+CGG 0.495093 8.1:+60802637 None:intergenic
TTTAGGAGAGATGACATTGG+AGG 0.495208 8.1:-60802732 MS.gene063953:CDS
GGGGATTTATATGCCTAGCC+AGG 0.498570 8.1:-60802543 MS.gene063953:CDS
TAAAGTTTCACGCCTGGAAG+AGG 0.502778 8.1:-60802163 MS.gene063953:CDS
CAGCCGTTACACATGGGGTC+TGG 0.513878 8.1:-60802512 MS.gene063953:CDS
GCCATATGACTATCTGCAAA+CGG 0.520435 8.1:+60802473 None:intergenic
GAAAAGGATGATCAAGAATC+GGG 0.521274 8.1:-60802348 MS.gene063953:CDS
CATGGGGTCTGGTGTTTCGA+TGG 0.522204 8.1:-60802501 MS.gene063953:CDS
ACTCAGACAATGGGGTCTCA+AGG 0.525496 8.1:-60803103 MS.gene063953:CDS
TGTTGTAGATGCTGATATTG+TGG 0.526222 8.1:+60802575 None:intergenic
AGTGAACCAGTCCACGAATA+CGG 0.529125 8.1:-60802915 MS.gene063953:CDS
CAAGGTGGTGGAGATGGTAG+TGG 0.530920 8.1:-60803085 MS.gene063953:CDS
GCATCTACAACAAAGTTCCA+TGG 0.531471 8.1:-60802564 MS.gene063953:CDS
AGTTTCCTCTGCAGGGTCAG+TGG 0.540111 8.1:-60802613 MS.gene063953:CDS
CATCTACAACAAAGTTCCAT+GGG 0.551430 8.1:-60802563 MS.gene063953:CDS
TAAAAGCTGCATTCAGCAAG+AGG 0.554122 8.1:-60802832 MS.gene063953:CDS
GGAGATTAAAGTTTCACGCC+TGG 0.555439 8.1:-60802169 MS.gene063953:CDS
AGATGCTGATATTGTGGTTG+CGG 0.557999 8.1:+60802581 None:intergenic
ACAATGGGGTCTCAAGGTGG+TGG 0.565619 8.1:-60803097 MS.gene063953:CDS
ACCTCTTATGGGAACTGTGT+CGG 0.579301 8.1:-60802441 MS.gene063953:CDS
TCCACGAATACGGACAATGA+AGG 0.580287 8.1:-60802905 MS.gene063953:CDS
CTGCTGCCCGTTCAAGAGCA+CGG 0.591970 8.1:-60802322 MS.gene063953:CDS
TATAGCACAGCCGTTACACA+TGG 0.595784 8.1:-60802519 MS.gene063953:CDS
TGGAGAAGACTGTTGAGAGA+AGG 0.597925 8.1:-60802373 MS.gene063953:CDS
GTGGAGATGGTAGTGGAAGA+CGG 0.609087 8.1:-60803078 MS.gene063953:CDS
ACACCAGACCCCATGTGTAA+CGG 0.609930 8.1:+60802509 None:intergenic
CACAACTCCACTCTCTTGTG+AGG 0.615377 8.1:-60803054 MS.gene063953:CDS
GTATCACAGTTTCCTCTGCA+GGG 0.615727 8.1:-60802620 MS.gene063953:CDS
TGAAATGCTGAGGAAACGAA+AGG 0.623305 8.1:-60802136 MS.gene063953:intron
GGCTGTGCTATACCCTGGCT+AGG 0.624273 8.1:+60802530 None:intergenic
GAGCACTCCAGAGGATATGG+TGG 0.628901 8.1:-60802393 MS.gene063953:CDS
TAGGTACACAGATCTCAGAA+TGG 0.634421 8.1:+60801218 None:intergenic
AAGAGGAAAATGAAATGCTG+AGG 0.635284 8.1:-60802146 MS.gene063953:CDS
TACTTTAGGAGAGATGACAT+TGG 0.639124 8.1:-60802735 MS.gene063953:CDS
GGGATTTATATGCCTAGCCA+GGG 0.641870 8.1:-60802542 MS.gene063953:CDS
TGTAACCACTGACCCTGCAG+AGG 0.647637 8.1:+60802608 None:intergenic
GTCTGCGGCCGATTCAAATG+TGG 0.651473 8.1:-60802645 MS.gene063953:CDS
GCTAGGCATATAAATCCCCA+TGG 0.652039 8.1:+60802547 None:intergenic
TAGCACAGCCGTTACACATG+GGG 0.656286 8.1:-60802517 MS.gene063953:CDS
GAATACTGATACTACGTCTG+CGG 0.684349 8.1:-60802660 MS.gene063953:CDS
CAGACAATGGGGTCTCAAGG+TGG 0.688721 8.1:-60803100 MS.gene063953:CDS
GTAACGGCTGTGCTATACCC+TGG 0.689448 8.1:+60802525 None:intergenic
ATAGCACAGCCGTTACACAT+GGG 0.689708 8.1:-60802518 MS.gene063953:CDS
TGCATTCAGCAAGAGGACTG+TGG 0.691129 8.1:-60802825 MS.gene063953:CDS
AAATGGGAACTCAGACAATG+GGG 0.712282 8.1:-60803111 MS.gene063953:CDS
ATCTACAACAAAGTTCCATG+GGG 0.739377 8.1:-60802562 MS.gene063953:CDS
CCAACAAAAGAAAGACAACG+AGG 0.748926 8.1:-60802783 MS.gene063953:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAACAAATCAAATAATA+AGG + chr8.1:60802091-60802110 None:intergenic 15.0%
!! AATAAATCAAATTCCAAATC+AGG + chr8.1:60802802-60802821 None:intergenic 20.0%
!! TAGTTACTGGATTATTTATT+AGG - chr8.1:60802558-60802577 MS.gene063953:CDS 20.0%
!!! AAAAGTTATTATTCAGGTTT+AGG + chr8.1:60802127-60802146 None:intergenic 20.0%
!!! AAAGTTATTATTCAGGTTTA+GGG + chr8.1:60802126-60802145 None:intergenic 20.0%
!!! ATTTTTACCTGTTAAAATCA+CGG - chr8.1:60802935-60802954 MS.gene063953:CDS 20.0%
!!! TGATTTGGAATTTGATTTAT+TGG - chr8.1:60802801-60802820 MS.gene063953:CDS 20.0%
! AAAATCAATTAGGTGACTTA+GGG - chr8.1:60801347-60801366 MS.gene063953:intron 25.0%
! GATAGAGACTTTATATAGTA+CGG + chr8.1:60802891-60802910 None:intergenic 25.0%
! GATGAAGTTCAAAATCAATT+AGG - chr8.1:60801337-60801356 MS.gene063953:intron 25.0%
! GCTTGTAAAAGTTATTATTC+AGG + chr8.1:60802133-60802152 None:intergenic 25.0%
! GTGTTAGATTTGAATGAAAT+AGG - chr8.1:60802477-60802496 MS.gene063953:CDS 25.0%
! TAATCTGAATAGTCAATTAG+AGG + chr8.1:60803002-60803021 None:intergenic 25.0%
! TTATAATTTCAGCACAAGAT+AGG - chr8.1:60802332-60802351 MS.gene063953:CDS 25.0%
!! GAATAATAACTTTTACAAGC+AGG - chr8.1:60802133-60802152 MS.gene063953:intron 25.0%
!! GTAGTCATTAAGAATTTTTC+AGG - chr8.1:60802617-60802636 MS.gene063953:CDS 25.0%
!! TTTTTACCTGTTAAAATCAC+GGG - chr8.1:60802936-60802955 MS.gene063953:CDS 25.0%
AATGTACAAACTACTCAATC+TGG + chr8.1:60802306-60802325 None:intergenic 30.0%
AGAAAAGGATGATCAAGAAT+CGG - chr8.1:60801992-60802011 MS.gene063953:intron 30.0%
ATACTTGATATTGCCTGATT+TGG - chr8.1:60802786-60802805 MS.gene063953:CDS 30.0%
ATGTACAAACTACTCAATCT+GGG + chr8.1:60802305-60802324 None:intergenic 30.0%
CAAAATCAATTAGGTGACTT+AGG - chr8.1:60801346-60801365 MS.gene063953:intron 30.0%
CATTCAATTAGCAGTATTTG+TGG - chr8.1:60802846-60802865 MS.gene063953:CDS 30.0%
TTAACAGGTAAAAATGACCA+TGG + chr8.1:60802930-60802949 None:intergenic 30.0%
! ACTAGGTAAAGATTAGTTAC+TGG - chr8.1:60802545-60802564 MS.gene063953:CDS 30.0%
! TGATATTTAGGTTCAGCAAT+TGG + chr8.1:60803081-60803100 None:intergenic 30.0%
! TTGAGTAGTTTGTACATTTG+TGG - chr8.1:60802308-60802327 MS.gene063953:intron 30.0%
AAGAGGAAAATGAAATGCTG+AGG - chr8.1:60802195-60802214 MS.gene063953:intron 35.0%
AATATTTGTCCTCAACATGC+AGG - chr8.1:60803029-60803048 MS.gene063953:CDS 35.0%
ACTAACATGCTCATTTCTGT+TGG + chr8.1:60802681-60802700 None:intergenic 35.0%
ATCTACAACAAAGTTCCATG+GGG - chr8.1:60801779-60801798 MS.gene063953:intron 35.0%
ATTCGACGTAGCTGATATTT+AGG + chr8.1:60803093-60803112 None:intergenic 35.0%
CATCTACAACAAAGTTCCAT+GGG - chr8.1:60801778-60801797 MS.gene063953:intron 35.0%
CTACGTAAAAGATAGAGCAT+AGG + chr8.1:60802601-60802620 None:intergenic 35.0%
CTATATGCACATTGTATCCA+TGG - chr8.1:60802910-60802929 MS.gene063953:CDS 35.0%
CTGCTCTCAATATACTACAA+TGG - chr8.1:60802724-60802743 MS.gene063953:CDS 35.0%
GAAAAGGATGATCAAGAATC+GGG - chr8.1:60801993-60802012 MS.gene063953:intron 35.0%
GAATAGTCAATTAGAGGTGA+AGG + chr8.1:60802996-60803015 None:intergenic 35.0%
GGTTGATCAATGGATCTATT+TGG + chr8.1:60802510-60802529 None:intergenic 35.0%
TGATACTGCCACATTTGAAT+CGG + chr8.1:60801707-60801726 None:intergenic 35.0%
TGTACAAACTACTCAATCTG+GGG + chr8.1:60802304-60802323 None:intergenic 35.0%
TGTTGTAGATGCTGATATTG+TGG + chr8.1:60801769-60801788 None:intergenic 35.0%
! AAATATCTGCTGCTTCATGA+TGG - chr8.1:60802759-60802778 MS.gene063953:CDS 35.0%
! ACGAGCTTCTGAAAAATGTA+TGG - chr8.1:60801395-60801414 MS.gene063953:intron 35.0%
! TACTTTAGGAGAGATGACAT+TGG - chr8.1:60801606-60801625 MS.gene063953:intron 35.0%
!! AGAGATATCAGGTTGATCAA+TGG + chr8.1:60802520-60802539 None:intergenic 35.0%
!! TTTGTTCCCGTGATTTTAAC+AGG + chr8.1:60802945-60802964 None:intergenic 35.0%
!!! GTTAGTTCTGACACAACTTT+TGG - chr8.1:60802695-60802714 MS.gene063953:CDS 35.0%
AATGCAAATGATGCATGCTG+AGG + chr8.1:60802421-60802440 None:intergenic 40.0%
AGATGCTGATATTGTGGTTG+CGG + chr8.1:60801763-60801782 None:intergenic 40.0%
CCAACAAAAGAAAGACAACG+AGG - chr8.1:60801558-60801577 MS.gene063953:intron 40.0%
CCTAGTGTATCAGAGATATC+AGG + chr8.1:60802531-60802550 None:intergenic 40.0%
CCTGATATCTCTGATACACT+AGG - chr8.1:60802528-60802547 MS.gene063953:CDS 40.0%
CGAGAGAGACAAACTACTTT+AGG - chr8.1:60801592-60801611 MS.gene063953:intron 40.0%
GAATACTGATACTACGTCTG+CGG - chr8.1:60801681-60801700 MS.gene063953:intron 40.0%
GATGACATTGGAGGATTTCT+TGG - chr8.1:60801618-60801637 MS.gene063953:intron 40.0%
GCAGATAGTCATATGGCATT+GGG - chr8.1:60801874-60801893 MS.gene063953:intron 40.0%
GCAGGCTTACACAAATGAAT+TGG - chr8.1:60802151-60802170 MS.gene063953:CDS 40.0%
GCATCTACAACAAAGTTCCA+TGG - chr8.1:60801777-60801796 MS.gene063953:intron 40.0%
GCCATATGACTATCTGCAAA+CGG + chr8.1:60801871-60801890 None:intergenic 40.0%
TAAAAGCTGCATTCAGCAAG+AGG - chr8.1:60801509-60801528 MS.gene063953:intron 40.0%
TACTCAATCTGGGGAATGAA+AGG + chr8.1:60802295-60802314 None:intergenic 40.0%
TCCGTTTGCAGATAGTCATA+TGG - chr8.1:60801867-60801886 MS.gene063953:intron 40.0%
TCTAGGTTCATACTGCTTAG+TGG + chr8.1:60801376-60801395 None:intergenic 40.0%
TCTGGGGAATGAAAGGAATT+AGG + chr8.1:60802288-60802307 None:intergenic 40.0%
TGAAATGCTGAGGAAACGAA+AGG - chr8.1:60802205-60802224 MS.gene063953:intron 40.0%
TGCAGATAGTCATATGGCAT+TGG - chr8.1:60801873-60801892 MS.gene063953:intron 40.0%
TTTAGGAGAGATGACATTGG+AGG - chr8.1:60801609-60801628 MS.gene063953:intron 40.0%
! AATTTTGCCTCACAAGAGAG+TGG + chr8.1:60801297-60801316 None:intergenic 40.0%
! CAGTCTCAGTTTCTGGATTA+AGG - chr8.1:60802364-60802383 MS.gene063953:CDS 40.0%
! CCTCGTTGTCTTTCTTTTGT+TGG + chr8.1:60801561-60801580 None:intergenic 40.0%
! GCATTTCATTTTCCTCTTCC+AGG + chr8.1:60802193-60802212 None:intergenic 40.0%
!! CATTTTTCAGAAGCTCGTCT+AGG + chr8.1:60801393-60801412 None:intergenic 40.0%
ACAGTCTCAGTCTCAGTTTC+TGG - chr8.1:60802357-60802376 MS.gene063953:CDS 45.0%
ACCTCTTATGGGAACTGTGT+CGG - chr8.1:60801900-60801919 MS.gene063953:intron 45.0%
AGTATCACAGTTTCCTCTGC+AGG - chr8.1:60801720-60801739 MS.gene063953:intron 45.0%
AGTGAACCAGTCCACGAATA+CGG - chr8.1:60801426-60801445 MS.gene063953:intron 45.0%
ATAGCACAGCCGTTACACAT+GGG - chr8.1:60801823-60801842 MS.gene063953:intron 45.0%
CTGTTGAGAGAAGGCAGAAA+AGG - chr8.1:60801977-60801996 MS.gene063953:intron 45.0%
GAGGATTTCTTGGTGAAAGC+AGG - chr8.1:60801628-60801647 MS.gene063953:intron 45.0%
GCTAGGCATATAAATCCCCA+TGG + chr8.1:60801797-60801816 None:intergenic 45.0%
GGAGATTAAAGTTTCACGCC+TGG - chr8.1:60802172-60802191 MS.gene063953:CDS 45.0%
GGGATTTATATGCCTAGCCA+GGG - chr8.1:60801799-60801818 MS.gene063953:intron 45.0%
GGTTCAGCAATTGGTGTACA+TGG + chr8.1:60803072-60803091 None:intergenic 45.0%
GTATCACAGTTTCCTCTGCA+GGG - chr8.1:60801721-60801740 MS.gene063953:intron 45.0%
TAAAGTTTCACGCCTGGAAG+AGG - chr8.1:60802178-60802197 MS.gene063953:CDS 45.0%
TATAGCACAGCCGTTACACA+TGG - chr8.1:60801822-60801841 MS.gene063953:intron 45.0%
TCCACGAATACGGACAATGA+AGG - chr8.1:60801436-60801455 MS.gene063953:intron 45.0%
TCTACAACGTCAAGCTAGTC+TGG - chr8.1:60801483-60801502 MS.gene063953:intron 45.0%
TGGAGAAGACTGTTGAGAGA+AGG - chr8.1:60801968-60801987 MS.gene063953:intron 45.0%
!! AAGAAAAAGGAGCACTCCAG+AGG - chr8.1:60801939-60801958 MS.gene063953:intron 45.0%
ACACCAGACCCCATGTGTAA+CGG + chr8.1:60801835-60801854 None:intergenic 50.0%
ACTCAGACAATGGGGTCTCA+AGG - chr8.1:60801238-60801257 MS.gene063953:CDS 50.0%
AGAGGACTGTGGATGAGGTT+TGG - chr8.1:60801527-60801546 MS.gene063953:intron 50.0%
CACAACTCCACTCTCTTGTG+AGG - chr8.1:60801287-60801306 MS.gene063953:CDS 50.0%
CATTGTCCGTATTCGTGGAC+TGG + chr8.1:60801435-60801454 None:intergenic 50.0%
CCTGTTTCCGTGCTCTTGAA+CGG + chr8.1:60802029-60802048 None:intergenic 50.0%
CGCAGATGCCTGGAAGAAAA+AGG - chr8.1:60801926-60801945 MS.gene063953:intron 50.0%
CTGTTTCCGTGCTCTTGAAC+GGG + chr8.1:60802028-60802047 None:intergenic 50.0%
GCATTGGGTGCACCTCTTAT+GGG - chr8.1:60801889-60801908 MS.gene063953:intron 50.0%
GCCTTCATTGTCCGTATTCG+TGG + chr8.1:60801440-60801459 None:intergenic 50.0%
GGGGATTTATATGCCTAGCC+AGG - chr8.1:60801798-60801817 MS.gene063953:intron 50.0%
GTCTTCTCCACCATATCCTC+TGG + chr8.1:60801958-60801977 None:intergenic 50.0%
TAGCACAGCCGTTACACATG+GGG - chr8.1:60801824-60801843 MS.gene063953:intron 50.0%
TCCGACACAGTTCCCATAAG+AGG + chr8.1:60801904-60801923 None:intergenic 50.0%
TGCATTCAGCAAGAGGACTG+TGG - chr8.1:60801516-60801535 MS.gene063953:intron 50.0%
! TCTCTAGCTCCTGCATGTTG+AGG + chr8.1:60803041-60803060 None:intergenic 50.0%
!! AAGGAGCACTCCAGAGGATA+TGG - chr8.1:60801945-60801964 MS.gene063953:intron 50.0%
!! GGAGTGCTCCTTTTTCTTCC+AGG + chr8.1:60801937-60801956 None:intergenic 50.0%
!! GTGGAGATGGTAGTGGAAGA+CGG - chr8.1:60801263-60801282 MS.gene063953:CDS 50.0%
ACAATGGGGTCTCAAGGTGG+TGG - chr8.1:60801244-60801263 MS.gene063953:CDS 55.0%
AGTTTCCTCTGCAGGGTCAG+TGG - chr8.1:60801728-60801747 MS.gene063953:intron 55.0%
CAGACAATGGGGTCTCAAGG+TGG - chr8.1:60801241-60801260 MS.gene063953:CDS 55.0%
CAGCAAGAGGACTGTGGATG+AGG - chr8.1:60801522-60801541 MS.gene063953:intron 55.0%
CATGGGGTCTGGTGTTTCGA+TGG - chr8.1:60801840-60801859 MS.gene063953:intron 55.0%
GAGCACTCCAGAGGATATGG+TGG - chr8.1:60801948-60801967 MS.gene063953:intron 55.0%
GTAACGGCTGTGCTATACCC+TGG + chr8.1:60801819-60801838 None:intergenic 55.0%
TGTAACCACTGACCCTGCAG+AGG + chr8.1:60801736-60801755 None:intergenic 55.0%
! CCGTTCAAGAGCACGGAAAC+AGG - chr8.1:60802026-60802045 MS.gene063953:intron 55.0%
! GGCATTGGGTGCACCTCTTA+TGG - chr8.1:60801888-60801907 MS.gene063953:intron 55.0%
! GTCTGCGGCCGATTCAAATG+TGG - chr8.1:60801696-60801715 MS.gene063953:intron 55.0%
!! CAAGGTGGTGGAGATGGTAG+TGG - chr8.1:60801256-60801275 MS.gene063953:CDS 55.0%
CAGCCGTTACACATGGGGTC+TGG - chr8.1:60801829-60801848 MS.gene063953:intron 60.0%
CTGCTGCCCGTTCAAGAGCA+CGG - chr8.1:60802019-60802038 MS.gene063953:intron 60.0%
GGCTGTGCTATACCCTGGCT+AGG + chr8.1:60801814-60801833 None:intergenic 60.0%
GTGTCGGATACGCAGATGCC+TGG - chr8.1:60801916-60801935 MS.gene063953:intron 60.0%
!! GGGTCTCAAGGTGGTGGAGA+TGG - chr8.1:60801250-60801269 MS.gene063953:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 60801232 60803131 60801232 ID=MS.gene063953
chr8.1 mRNA 60801232 60803131 60801232 ID=MS.gene063953.t1;Parent=MS.gene063953
chr8.1 exon 60802316 60803131 60802316 ID=MS.gene063953.t1.exon1;Parent=MS.gene063953.t1
chr8.1 CDS 60802316 60803131 60802316 ID=cds.MS.gene063953.t1;Parent=MS.gene063953.t1
chr8.1 exon 60802137 60802208 60802137 ID=MS.gene063953.t1.exon2;Parent=MS.gene063953.t1
chr8.1 CDS 60802137 60802208 60802137 ID=cds.MS.gene063953.t1;Parent=MS.gene063953.t1
chr8.1 exon 60801232 60801312 60801232 ID=MS.gene063953.t1.exon3;Parent=MS.gene063953.t1
chr8.1 CDS 60801232 60801312 60801232 ID=cds.MS.gene063953.t1;Parent=MS.gene063953.t1
Gene Sequence

>MS.gene063953

ATGGGAACTCAGACAATGGGGTCTCAAGGTGGTGGAGATGGTAGTGGAAGACGGTCACAACTCCACTCTCTTGTGAGGCAAAATTCAGTGTACAGTCTCACACTAGATGAAGTTCAAAATCAATTAGGTGACTTAGGGAAGCCACTAAGCAGTATGAACCTAGACGAGCTTCTGAAAAATGTATGGACTGTTGAAGTGAACCAGTCCACGAATACGGACAATGAAGGCACAACGCAATCTAGTGAAGCTTCTCTACAACGTCAAGCTAGTCTGGCATTAAAAGCTGCATTCAGCAAGAGGACTGTGGATGAGGTTTGGAGAGATATCCAACAAAAGAAAGACAACGAGGAAAAGAAGTCACGAGAGAGACAAACTACTTTAGGAGAGATGACATTGGAGGATTTCTTGGTGAAAGCAGGTATTGTTGCAGAAGCATCTTCTAATAAAAAGAATACTGATACTACGTCTGCGGCCGATTCAAATGTGGCAGTATCACAGTTTCCTCTGCAGGGTCAGTGGTTACAGTATCCGCAACCACAATATCAGCATCTACAACAAAGTTCCATGGGGATTTATATGCCTAGCCAGGGTATAGCACAGCCGTTACACATGGGGTCTGGTGTTTCGATGGAGATTCCGTTTGCAGATAGTCATATGGCATTGGGTGCACCTCTTATGGGAACTGTGTCGGATACGCAGATGCCTGGAAGAAAAAGGAGCACTCCAGAGGATATGGTGGAGAAGACTGTTGAGAGAAGGCAGAAAAGGATGATCAAGAATCGGGAATCTGCTGCCCGTTCAAGAGCACGGAAACAGGTGATCTCTCTCTTTATTTCGAGTGTTGTAACTCGCAAGTCCTTATTATTTGATTTGTTTTTGTTGTAATGAATACCCTAAACCTGAATAATAACTTTTACAAGCAGGCTTACACAAATGAATTGGAGATTAAAGTTTCACGCCTGGAAGAGGAAAATGAAATGCTGAGGAAACGAAAGGTTTGATTCTGCTTTGAAATTATAAGTTTAATTGATTTGAGTATGTTTTTATGATGATCCTAATTCCTTTCATTCCCCAGATTGAGTAGTTTGTACATTTGTGGATTATAATTTCAGCACAAGATAGGAGACAGTCTCAGTCTCAGTTTCTGGATTAAGGAAACTCTGCATATTATGTTCTCTAGTTGAGACCTCAGCATGCATCATTTGCATTATGCATCTTTTCTCTCTTTAACTCTCATAATTGATTGTGTTAGATTTGAATGAAATAGGAATAGATCCAAATAGATCCATTGATCAACCTGATATCTCTGATACACTAGGTAAAGATTAGTTACTGGATTATTTATTAGGATTTATCTCTCTTATGACCTATGCTCTATCTTTTACGTAGTCATTAAGAATTTTTCAGGAAAATGAATCACATAGTCACTTTTAATTTTGTGATCGACCAACAGAAATGAGCATGTTAGTTCTGACACAACTTTTGGATTATTCTGCTCTCAATATACTACAATGGATTATAAATTTTAAATATCTGCTGCTTCATGATGGTTGTATACTTGATATTGCCTGATTTGGAATTTGATTTATTGGCTCAACATTGTTCACTCTTTTTCATTCAATTAGCAGTATTTGTGGAAGATCTTGCATCGCACAACCGTACTATATAAAGTCTCTATCTATATGCACATTGTATCCATGGTCATTTTTACCTGTTAAAATCACGGGAACAAAATTCTTCTTTTATACATGCTAACTTGCTCCTTCACCTCTAATTGACTATTCAGATTATATTATTAATATTTGTCCTCAACATGCAGGAGCTAGAGAGTATGTTGCCATGTACACCAATTGCTGAACCTAAATATCAGCTACGTCGAATAGCATCTTGTCCATTCTGA
Protein sequence

>MS.gene063953.t1

MGTQTMGSQGGGDGSGRRSQLHSLVRQNSVYSLTLDEVQNQLGDLGKPLSSMNLDELLKNVWTVEVNQSTNTDNEGTTQSSEASLQRQASLALKAAFSKRTVDEVWRDIQQKKDNEEKKSRERQTTLGEMTLEDFLVKAGIVAEASSNKKNTDTTSAADSNVAVSQFPLQGQWLQYPQPQYQHLQQSSMGIYMPSQGIAQPLHMGSGVSMEIPFADSHMALGAPLMGTVSDTQMPGRKRSTPEDMVEKTVERRQKRMIKNRESAARSRARKQAYTNELEIKVSRLEEENEMLRKRKELESMLPCTPIAEPKYQLRRIASCPF