Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064056.t1 | XP_003620925.1 | 90.5 | 168 | 16 | 0 | 9 | 176 | 136 | 303 | 1.80E-81 | 312 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064056.t1 | F4K933 | 41.0 | 161 | 91 | 2 | 5 | 161 | 147 | 307 | 4.0e-26 | 119.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064056.t1 | G7KJT8 | 90.5 | 168 | 16 | 0 | 9 | 176 | 136 | 303 | 1.3e-81 | 312.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene064056.t1 | TF | HRT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064056.t1 | MTR_6g092520 | 88.889 | 171 | 19 | 0 | 6 | 176 | 133 | 303 | 7.44e-110 | 318 |
| MS.gene064056.t1 | MTR_7g090060 | 70.482 | 166 | 48 | 1 | 6 | 170 | 137 | 302 | 3.33e-78 | 238 |
| MS.gene064056.t1 | MTR_6g092480 | 38.889 | 108 | 51 | 2 | 6 | 99 | 21 | 127 | 1.90e-17 | 79.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064056.t1 | AT5G56780 | 40.741 | 162 | 92 | 2 | 4 | 161 | 146 | 307 | 7.33e-32 | 119 |
Find 30 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCAACATACTTGATTTAAT+TGG | 0.224850 | 6.1:+79547157 | None:intergenic |
| TAAAATTTGTGGAGTGAATT+TGG | 0.231337 | 6.1:-79547005 | MS.gene064056:CDS |
| GTTGTATCTTGAACTATCTT+AGG | 0.344160 | 6.1:+79547052 | None:intergenic |
| AGTGAAGGGTAGAAGTGATT+TGG | 0.349328 | 6.1:+79547198 | None:intergenic |
| TCGGATTTGTTATCTTTCAT+AGG | 0.354876 | 6.1:+79547136 | None:intergenic |
| ATGATTCTAGCTTCTTTCAT+TGG | 0.358373 | 6.1:-79548012 | MS.gene064056:CDS |
| GGAATTTGAACACGCGAGAT+AGG | 0.386624 | 6.1:+79547086 | None:intergenic |
| TGTTTGCAAGGCATCTCCCT+TGG | 0.436980 | 6.1:+79547348 | None:intergenic |
| ACTGCCATTGTCTGCTTCAT+CGG | 0.450271 | 6.1:+79547117 | None:intergenic |
| GAGAAGAGTGGTAAAATTTG+TGG | 0.450994 | 6.1:-79547016 | MS.gene064056:CDS |
| ATCTGAAAATGTTCTTGTGC+TGG | 0.457395 | 6.1:+79547225 | None:intergenic |
| GTTAGGTGTCATGAACATAA+AGG | 0.469142 | 6.1:-79546941 | MS.gene064056:CDS |
| TGAGTACATTTGATTATGCT+TGG | 0.471865 | 6.1:-79547312 | MS.gene064056:CDS |
| CAGTTGAGATTCTGTTTGCA+AGG | 0.488213 | 6.1:+79547336 | None:intergenic |
| GCTTGGAACACAATTAATAA+CGG | 0.491690 | 6.1:-79547295 | MS.gene064056:CDS |
| TTCTAGCTTCTTTCATTGGA+AGG | 0.495725 | 6.1:-79548008 | MS.gene064056:intron |
| CCTTCACTCAAAAGAAAGTA+GGG | 0.503569 | 6.1:-79547183 | MS.gene064056:CDS |
| CCCTTCACTCAAAAGAAAGT+AGG | 0.510670 | 6.1:-79547184 | MS.gene064056:CDS |
| GAGCATTTCAAGAATATCAC+CGG | 0.525607 | 6.1:+79547261 | None:intergenic |
| CTATCTTAGGACGCGACCGG+TGG | 0.570672 | 6.1:+79547065 | None:intergenic |
| AATAACGGTCCACGGCGTCC+CGG | 0.572061 | 6.1:-79547280 | MS.gene064056:CDS |
| CTCGCGTGTTCAAATTCCAC+CGG | 0.598427 | 6.1:-79547081 | MS.gene064056:CDS |
| GAACTATCTTAGGACGCGAC+CGG | 0.598468 | 6.1:+79547062 | None:intergenic |
| TTCTATTTGTACAAAGACGC+CGG | 0.623541 | 6.1:-79546975 | MS.gene064056:CDS |
| AGAATATCACCGGGACGCCG+TGG | 0.625598 | 6.1:+79547271 | None:intergenic |
| GTTGAACAGATGCAAACCAA+GGG | 0.638402 | 6.1:-79547364 | MS.gene064056:intron |
| ACACAATTAATAACGGTCCA+CGG | 0.642408 | 6.1:-79547288 | MS.gene064056:CDS |
| TGTTGAACAGATGCAAACCA+AGG | 0.647561 | 6.1:-79547365 | MS.gene064056:intron |
| AGCATTTCAAGAATATCACC+GGG | 0.651967 | 6.1:+79547262 | None:intergenic |
| AAATCCGATGAAGCAGACAA+TGG | 0.675612 | 6.1:-79547121 | MS.gene064056:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCTAATAGAATAAACAAT+TGG | - | chr6.1:79546932-79546951 | MS.gene064056:CDS | 15.0% |
| !!! | GCATTTATATTTAATCTTTT+GGG | + | chr6.1:79547448-79547467 | None:intergenic | 15.0% |
| !!! | TGCATTTATATTTAATCTTT+TGG | + | chr6.1:79547449-79547468 | None:intergenic | 15.0% |
| !! | ATAATATGTATCTCAAATCA+TGG | + | chr6.1:79547190-79547209 | None:intergenic | 20.0% |
| !! | CAAAATGAACATATACAATA+TGG | + | chr6.1:79547161-79547180 | None:intergenic | 20.0% |
| !! | TGTTTATTCTATTAGATTAG+AGG | + | chr6.1:79546930-79546949 | None:intergenic | 20.0% |
| !! | TTAATTAAGAGCTAAGTTAT+AGG | - | chr6.1:79547270-79547289 | MS.gene064056:CDS | 20.0% |
| !!! | TTTTCCAATTGTTTATAGAT+GGG | - | chr6.1:79547019-79547038 | MS.gene064056:CDS | 20.0% |
| ! | AAATTAAGAGACATGATTCA+AGG | - | chr6.1:79546996-79547015 | MS.gene064056:CDS | 25.0% |
| ! | TAAAATTTGTGGAGTGAATT+TGG | - | chr6.1:79547890-79547909 | MS.gene064056:intron | 25.0% |
| ! | TCAACTTATTAAGATCAAGA+TGG | - | chr6.1:79547081-79547100 | MS.gene064056:CDS | 25.0% |
| !! | GTTTTCCAATTGTTTATAGA+TGG | - | chr6.1:79547018-79547037 | MS.gene064056:CDS | 25.0% |
| !! | TATGAAGTAAGTATTTTCTC+TGG | - | chr6.1:79547325-79547344 | MS.gene064056:CDS | 25.0% |
| AATGCATAGAATGTACTTAC+AGG | + | chr6.1:79547047-79547066 | None:intergenic | 30.0% | |
| CACATTTGCATCTTACATAA+AGG | - | chr6.1:79547467-79547486 | MS.gene064056:intron | 30.0% | |
| GAACATATACAATATGGTCA+AGG | + | chr6.1:79547155-79547174 | None:intergenic | 30.0% | |
| GCTTGGAACACAATTAATAA+CGG | - | chr6.1:79547600-79547619 | MS.gene064056:intron | 30.0% | |
| GGCAACATACTTGATTTAAT+TGG | + | chr6.1:79547741-79547760 | None:intergenic | 30.0% | |
| GTATTAAGAGACATGATTCA+AGG | + | chr6.1:79546962-79546981 | None:intergenic | 30.0% | |
| GTTGTATCTTGAACTATCTT+AGG | + | chr6.1:79547846-79547865 | None:intergenic | 30.0% | |
| TAAGAGCTAAGTTATAGGTT+TGG | - | chr6.1:79547275-79547294 | MS.gene064056:CDS | 30.0% | |
| TGAGTACATTTGATTATGCT+TGG | - | chr6.1:79547583-79547602 | MS.gene064056:intron | 30.0% | |
| ! | AATTTTACCACTCTTCTCAA+CGG | + | chr6.1:79547877-79547896 | None:intergenic | 30.0% |
| !! | ATGATTCTAGCTTCTTTCAT+TGG | - | chr6.1:79546883-79546902 | MS.gene064056:CDS | 30.0% |
| !! | TCGGATTTGTTATCTTTCAT+AGG | + | chr6.1:79547762-79547781 | None:intergenic | 30.0% |
| ACACAATTAATAACGGTCCA+CGG | - | chr6.1:79547607-79547626 | MS.gene064056:intron | 35.0% | |
| AGCATTTCAAGAATATCACC+GGG | + | chr6.1:79547636-79547655 | None:intergenic | 35.0% | |
| AGTTATAGGTTTGGAAGTCT+AGG | - | chr6.1:79547284-79547303 | MS.gene064056:CDS | 35.0% | |
| ATACAATATGGTCAAGGCAA+TGG | + | chr6.1:79547149-79547168 | None:intergenic | 35.0% | |
| ATCTGAAAATGTTCTTGTGC+TGG | + | chr6.1:79547673-79547692 | None:intergenic | 35.0% | |
| CAAAAAGAGGAAATGCTTGT+AGG | - | chr6.1:79548006-79548025 | MS.gene064056:intron | 35.0% | |
| CCTTCACTCAAAAGAAAGTA+GGG | - | chr6.1:79547712-79547731 | MS.gene064056:intron | 35.0% | |
| GAGAAGAGTGGTAAAATTTG+TGG | - | chr6.1:79547879-79547898 | MS.gene064056:intron | 35.0% | |
| GAGCATTTCAAGAATATCAC+CGG | + | chr6.1:79547637-79547656 | None:intergenic | 35.0% | |
| TCCAAGTCAGAATCAAAAAG+AGG | - | chr6.1:79547993-79548012 | MS.gene064056:intron | 35.0% | |
| TTCTATTTGTACAAAGACGC+CGG | - | chr6.1:79547920-79547939 | MS.gene064056:intron | 35.0% | |
| ! | GTTAGGTGTCATGAACATAA+AGG | - | chr6.1:79547954-79547973 | MS.gene064056:intron | 35.0% |
| !! | CCTACTTTCTTTTGAGTGAA+GGG | + | chr6.1:79547714-79547733 | None:intergenic | 35.0% |
| !! | TCCTCTTTTTGATTCTGACT+TGG | + | chr6.1:79547997-79548016 | None:intergenic | 35.0% |
| !! | TTCTAGCTTCTTTCATTGGA+AGG | - | chr6.1:79546887-79546906 | MS.gene064056:CDS | 35.0% |
| AAATCCGATGAAGCAGACAA+TGG | - | chr6.1:79547774-79547793 | MS.gene064056:intron | 40.0% | |
| AGTGAAGGGTAGAAGTGATT+TGG | + | chr6.1:79547700-79547719 | None:intergenic | 40.0% | |
| CAGTTGAGATTCTGTTTGCA+AGG | + | chr6.1:79547562-79547581 | None:intergenic | 40.0% | |
| CCCTTCACTCAAAAGAAAGT+AGG | - | chr6.1:79547711-79547730 | MS.gene064056:intron | 40.0% | |
| GGAGCCCATCTATAAACAAT+TGG | + | chr6.1:79547026-79547045 | None:intergenic | 40.0% | |
| GTTGAACAGATGCAAACCAA+GGG | - | chr6.1:79547531-79547550 | MS.gene064056:intron | 40.0% | |
| TGTTGAACAGATGCAAACCA+AGG | - | chr6.1:79547530-79547549 | MS.gene064056:intron | 40.0% | |
| ! | GATTTTTCCGTTGAGAAGAG+TGG | - | chr6.1:79547867-79547886 | MS.gene064056:intron | 40.0% |
| !! | CCCTACTTTCTTTTGAGTGA+AGG | + | chr6.1:79547715-79547734 | None:intergenic | 40.0% |
| !!! | CACCTAACTCTTTTTTCGAC+CGG | + | chr6.1:79547942-79547961 | None:intergenic | 40.0% |
| ACTGCCATTGTCTGCTTCAT+CGG | + | chr6.1:79547781-79547800 | None:intergenic | 45.0% | |
| GGAATTTGAACACGCGAGAT+AGG | + | chr6.1:79547812-79547831 | None:intergenic | 45.0% | |
| CGCCGGTCGAAAAAAGAGTT+AGG | - | chr6.1:79547937-79547956 | MS.gene064056:intron | 50.0% | |
| CTCGCGTGTTCAAATTCCAC+CGG | - | chr6.1:79547814-79547833 | MS.gene064056:intron | 50.0% | |
| GAACTATCTTAGGACGCGAC+CGG | + | chr6.1:79547836-79547855 | None:intergenic | 50.0% | |
| ! | TGTTTGCAAGGCATCTCCCT+TGG | + | chr6.1:79547550-79547569 | None:intergenic | 50.0% |
| AATAACGGTCCACGGCGTCC+CGG | - | chr6.1:79547615-79547634 | MS.gene064056:intron | 60.0% | |
| AGAATATCACCGGGACGCCG+TGG | + | chr6.1:79547627-79547646 | None:intergenic | 60.0% | |
| CTATCTTAGGACGCGACCGG+TGG | + | chr6.1:79547833-79547852 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 79546883 | 79548034 | 79546883 | ID=MS.gene064056 |
| chr6.1 | mRNA | 79546883 | 79548034 | 79546883 | ID=MS.gene064056.t1;Parent=MS.gene064056 |
| chr6.1 | exon | 79548009 | 79548034 | 79548009 | ID=MS.gene064056.t1.exon1;Parent=MS.gene064056.t1 |
| chr6.1 | CDS | 79548009 | 79548034 | 79548009 | ID=cds.MS.gene064056.t1;Parent=MS.gene064056.t1 |
| chr6.1 | exon | 79547871 | 79547880 | 79547871 | ID=MS.gene064056.t1.exon2;Parent=MS.gene064056.t1 |
| chr6.1 | CDS | 79547871 | 79547880 | 79547871 | ID=cds.MS.gene064056.t1;Parent=MS.gene064056.t1 |
| chr6.1 | exon | 79546883 | 79547377 | 79546883 | ID=MS.gene064056.t1.exon3;Parent=MS.gene064056.t1 |
| chr6.1 | CDS | 79546883 | 79547377 | 79546883 | ID=cds.MS.gene064056.t1;Parent=MS.gene064056.t1 |
| Gene Sequence |
| Protein sequence |
