AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064181

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064181.t1 MTR_0072s0040 97.131 244 2 2 1 244 1 239 5.56e-175 481
MS.gene064181.t1 MTR_2g090080 75.519 241 54 2 4 244 1 236 7.16e-135 379
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064181.t1 AT4G28190 73.276 232 57 2 13 244 11 237 4.88e-125 354
MS.gene064181.t1 AT4G28190 64.639 263 57 3 13 244 11 268 2.38e-118 338
MS.gene064181.t1 AT2G20825 68.122 229 62 3 16 244 10 227 9.99e-114 325

Find 55 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGACCAACCCGGCCATTT+TGG 0.182520 4.2:-56231826 None:intergenic
AATATCTGGGTTATAATTAA+TGG 0.255756 4.2:+56231722 MS.gene064181:CDS
GCACTTCTTGTGTGTGCTTT+GGG 0.273100 4.2:+56233595 MS.gene064181:CDS
TGCACTTCTTGTGTGTGCTT+TGG 0.279825 4.2:+56233594 MS.gene064181:CDS
GGAAATGGAAGAATAATATC+TGG 0.324881 4.2:+56231708 MS.gene064181:CDS
AATGGGTTGGAGAAGGGAAA+TGG 0.345803 4.2:+56229742 MS.gene064181:CDS
CTGCTGTGTCAGATGCAAAT+TGG 0.357390 4.2:+56231936 MS.gene064181:CDS
ATGAGTTTGTTCGCTGTACT+AGG 0.392965 4.2:+56231852 MS.gene064181:CDS
GGCTCTCAGAGATCCCAAAA+TGG 0.420579 4.2:+56231812 MS.gene064181:CDS
ATGGGTTGGAGAAGGGAAAT+GGG 0.422445 4.2:+56229743 MS.gene064181:CDS
GGTTATAATTAATGGTGATA+AGG 0.431324 4.2:+56231730 MS.gene064181:CDS
GAAGAGAGACAGCAAGGAAA+TGG 0.432232 4.2:+56231693 MS.gene064181:CDS
GAATAAACGTACTTCTCATA+TGG 0.434948 4.2:-56231974 None:intergenic
CAAGTGGCGAAAGCTGCTAA+TGG 0.441867 4.2:+56231791 MS.gene064181:CDS
TTTATGGAGAAAATGGAGAA+TGG 0.445958 4.2:+56229724 None:intergenic
TGGTAAAGTCAGTACAAGTT+TGG 0.447509 4.2:-56233650 None:intergenic
TTATGGAGAAAATGGAGAAT+GGG 0.453953 4.2:+56229725 None:intergenic
AAGAGCTGAGAGAAATGAGT+GGG 0.458242 4.2:+56229785 MS.gene064181:CDS
TAATGGTGATAAGGTTCCAT+TGG 0.460779 4.2:+56231739 MS.gene064181:CDS
TGCACTCCTGGTTGTGATGA+AGG 0.473540 4.2:+56229925 MS.gene064181:CDS
ATGGAGAATGGGTTGGAGAA+GGG 0.474047 4.2:+56229736 MS.gene064181:CDS
TGATGCTGTTGGTAGACTTA+GGG 0.474100 4.2:+56229867 MS.gene064181:CDS
AACCTTTGCACGTTGGTGAT+CGG 0.474249 4.2:-56233572 None:intergenic
TGCATTCACAAATAATTTGA+AGG 0.477500 4.2:-56229906 None:intergenic
CTCAGAGATCCCAAAATGGC+CGG 0.484048 4.2:+56231816 MS.gene064181:CDS
AATGGTGATAAGGTTCCATT+GGG 0.489767 4.2:+56231740 MS.gene064181:CDS
AATGGAGAATGGGTTGGAGA+AGG 0.494806 4.2:+56229735 MS.gene064181:CDS
GTGATGCTGTTGGTAGACTT+AGG 0.502004 4.2:+56229866 MS.gene064181:CDS
ATTATTTGTGAATGCACTCC+TGG 0.510243 4.2:+56229913 MS.gene064181:CDS
GGAGAAAATGGAGAATGGGT+TGG 0.523981 4.2:+56229729 MS.gene064181:CDS
ATTTCGTCTGAGATCTAAAG+AGG 0.537276 4.2:+56231892 MS.gene064181:CDS
GGCCGATCACCAACGTGCAA+AGG 0.545531 4.2:+56233570 MS.gene064181:CDS
AGATCCCAAAATGGCCGGGT+TGG 0.546651 4.2:+56231821 MS.gene064181:CDS
GAGTGTTTCTCAAATGCAGC+AGG 0.552189 4.2:-56231668 None:intergenic
TCAGAGATCCCAAAATGGCC+GGG 0.567774 4.2:+56231817 MS.gene064181:CDS
CATCTGACACAGCAGCATCA+TGG 0.577346 4.2:-56231928 None:intergenic
GAAATGGAAGAATAATATCT+GGG 0.580008 4.2:+56231709 MS.gene064181:CDS
CGAAGAGTTTACAGAGGATG+CGG 0.588649 4.2:+56233549 MS.gene064181:CDS
GCTGCATTTGAGAAACACTC+TGG 0.595645 4.2:+56231671 MS.gene064181:CDS
GGGTGTACTAGTCATAGATA+TGG 0.599115 4.2:+56229844 MS.gene064181:CDS
ACTCATCGCGGTGACCAACC+CGG 0.606791 4.2:-56231835 None:intergenic
CAGCAGCATCATGGTGAATG+CGG 0.613158 4.2:-56231919 None:intergenic
AGAGCTGAGAGAAATGAGTG+GGG 0.614369 4.2:+56229786 MS.gene064181:CDS
CATAGATATGGTGATGCTGT+TGG 0.619627 4.2:+56229856 MS.gene064181:CDS
GCTCAAATACTACAATCAAG+TGG 0.629351 4.2:+56231775 MS.gene064181:CDS
TGGGTTGTCAATGTTTAGCG+AGG 0.646931 4.2:+56229762 MS.gene064181:CDS
GAAGAGCTGAGAGAAATGAG+TGG 0.646981 4.2:+56229784 MS.gene064181:CDS
GGAAGTCGAAGAGTTTACAG+AGG 0.661639 4.2:+56233543 MS.gene064181:CDS
ACTCTGGAAGAGAGACAGCA+AGG 0.669740 4.2:+56231687 MS.gene064181:CDS
GAAGTGCAACCTTTGCACGT+TGG 0.672088 4.2:-56233579 None:intergenic
GAATATGTTGAAGTCACGTG+TGG 0.677819 4.2:+56229823 MS.gene064181:CDS
GAGGGACCTTCATCACAACC+AGG 0.689911 4.2:-56229931 None:intergenic
TGTGATGATGAAGAAGAGCG+AGG 0.697305 4.2:+56233522 MS.gene064181:CDS
AATATGTTGAAGTCACGTGT+GGG 0.722833 4.2:+56229824 MS.gene064181:CDS
TACAGCGAACAAACTCATCG+CGG 0.760016 4.2:-56231847 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACAAAATCATAAATTAT+TGG - chr4.2:56231434-56231453 None:intergenic 10.0%
!! ATATATATAATCTTTGAATT+TGG + chr4.2:56232351-56232370 MS.gene064181:intron 10.0%
!! ATTGTATGAAAATTTATAAT+TGG + chr4.2:56230346-56230365 MS.gene064181:intron 10.0%
!! TATATATAATCTTTGAATTT+GGG + chr4.2:56232352-56232371 MS.gene064181:intron 10.0%
!!! AATAATTTATGATTTTGTTT+TGG + chr4.2:56231433-56231452 MS.gene064181:intron 10.0%
!!! CTTCTTTTATTTATATTTTT+AGG - chr4.2:56231249-56231268 None:intergenic 10.0%
!!! TTTTTCAAATTAAATTATGA+AGG - chr4.2:56231167-56231186 None:intergenic 10.0%
!! TTATATATTAACAATACCTT+AGG - chr4.2:56230557-56230576 None:intergenic 15.0%
!!! CATTTTTATGGTAATAATTA+AGG - chr4.2:56233455-56233474 None:intergenic 15.0%
!!! CTAAATATATGTCATTTTTA+TGG - chr4.2:56233467-56233486 None:intergenic 15.0%
!!! TAATTTTTTGACCAAATTTA+AGG + chr4.2:56233209-56233228 MS.gene064181:intron 15.0%
!!! TTTTATAATAAACCATAAGT+TGG - chr4.2:56233313-56233332 None:intergenic 15.0%
!!! TTTTATTGGTATTTTGTTAT+TGG + chr4.2:56232747-56232766 MS.gene064181:intron 15.0%
!!! TTTTTTGTTTTTATCTAAGT+CGG + chr4.2:56230105-56230124 MS.gene064181:intron 15.0%
!! AAAAGACCAAATTTATGAAT+TGG - chr4.2:56232506-56232525 None:intergenic 20.0%
!! AACATCATATGCAAAAATTA+AGG - chr4.2:56231626-56231645 None:intergenic 20.0%
!! AATATCTGGGTTATAATTAA+TGG + chr4.2:56231722-56231741 MS.gene064181:CDS 20.0%
!! AATTTGAAAAATCAACAACT+TGG + chr4.2:56231176-56231195 MS.gene064181:intron 20.0%
!! ACATGGAAAAAAATATACAA+TGG - chr4.2:56231503-56231522 None:intergenic 20.0%
!! ATATAAACATGACATTACAA+CGG - chr4.2:56230839-56230858 None:intergenic 20.0%
!! ATATAAGTATTTATGCATGT+CGG - chr4.2:56230046-56230065 None:intergenic 20.0%
!! ATATGACTAATTATTGAATG+TGG + chr4.2:56231337-56231356 MS.gene064181:intron 20.0%
!! ATTATAAAAGACTCAGAATT+TGG + chr4.2:56233324-56233343 MS.gene064181:intron 20.0%
!! ATTTGAAAAATCAACAACTT+GGG + chr4.2:56231177-56231196 MS.gene064181:intron 20.0%
!! CAAATTAAATTATGAAGGTA+TGG - chr4.2:56231162-56231181 None:intergenic 20.0%
!! GTATAGTAATTAATATCCTA+AGG + chr4.2:56230538-56230557 MS.gene064181:intron 20.0%
!! TCAATAATTAGTCATATAGA+CGG - chr4.2:56231334-56231353 None:intergenic 20.0%
!! TTACATATAAGTAGTGTTAA+CGG + chr4.2:56231579-56231598 MS.gene064181:intron 20.0%
!! TTATAAAAGACTCAGAATTT+GGG + chr4.2:56233325-56233344 MS.gene064181:intron 20.0%
!! TTATAGATATGGTTATCATT+TGG + chr4.2:56233241-56233260 MS.gene064181:intron 20.0%
!! TTGTGAGATTAAATTCAATA+TGG + chr4.2:56230381-56230400 MS.gene064181:intron 20.0%
!!! AAAACTAATAGCCTTAAATT+TGG - chr4.2:56233223-56233242 None:intergenic 20.0%
!!! AATCTTTTGCATAAAATTTC+AGG + chr4.2:56232714-56232733 MS.gene064181:intron 20.0%
!!! ATAATTGATAGTCTTAATCT+AGG + chr4.2:56232600-56232619 MS.gene064181:intron 20.0%
!!! CAGGATGAAATATATTTTAT+TGG + chr4.2:56232733-56232752 MS.gene064181:intron 20.0%
! AATTAAGTGGTATGAATATG+AGG - chr4.2:56230664-56230683 None:intergenic 25.0%
! AGCAGTTATTTATCATAGTT+CGG - chr4.2:56232693-56232712 None:intergenic 25.0%
! AGTAGTTGAACAGAAAATAA+AGG - chr4.2:56233278-56233297 None:intergenic 25.0%
! ATAGATCTTAGAATTTGTGT+TGG + chr4.2:56232141-56232160 MS.gene064181:intron 25.0%
! CTGAAAATGAGTTTATAAGT+GGG - chr4.2:56232416-56232435 None:intergenic 25.0%
! GAAATGGAAGAATAATATCT+GGG + chr4.2:56231709-56231728 MS.gene064181:CDS 25.0%
! GGTTATAATTAATGGTGATA+AGG + chr4.2:56231730-56231749 MS.gene064181:CDS 25.0%
! GTGAAAATGAGTTTATAAGT+GGG - chr4.2:56232212-56232231 None:intergenic 25.0%
! GTTAAGCCAATTCATAAATT+TGG + chr4.2:56232497-56232516 MS.gene064181:intron 25.0%
! GTTGTAATGTCATGTTTATA+TGG + chr4.2:56230838-56230857 MS.gene064181:intron 25.0%
! TAATAAAACAACACACATAG+TGG - chr4.2:56230906-56230925 None:intergenic 25.0%
! TAGATCTTAGAATTTGTGTT+GGG + chr4.2:56232142-56232161 MS.gene064181:intron 25.0%
! TATAATCTTTGAATTTGGGT+TGG + chr4.2:56232356-56232375 MS.gene064181:intron 25.0%
! TCATTCATATATCCAATGAT+AGG + chr4.2:56231462-56231481 MS.gene064181:intron 25.0%
! TCTGAAAATGAGTTTATAAG+TGG - chr4.2:56232417-56232436 None:intergenic 25.0%
! TGAGAATTTGTGATTTAAAC+TGG + chr4.2:56231003-56231022 MS.gene064181:intron 25.0%
! TGATATATACAAGTAGAACT+TGG + chr4.2:56230935-56230954 MS.gene064181:intron 25.0%
! TGCACTTGTACTATTTATTT+AGG + chr4.2:56233396-56233415 MS.gene064181:intron 25.0%
! TGCATTCACAAATAATTTGA+AGG - chr4.2:56229909-56229928 None:intergenic 25.0%
! TGTATGTGTTAGTATTTCAT+AGG + chr4.2:56230148-56230167 MS.gene064181:intron 25.0%
! TTCTTAAAGTTACTAAGTCT+TGG + chr4.2:56232784-56232803 MS.gene064181:intron 25.0%
!! AAAGAACATACATAACTTGT+AGG - chr4.2:56230580-56230599 None:intergenic 25.0%
!! ATAATCTTTGAATTTGGGTT+GGG + chr4.2:56232357-56232376 MS.gene064181:intron 25.0%
!! ATACTTGATAGTATATGGTT+TGG + chr4.2:56230174-56230193 MS.gene064181:intron 25.0%
!! ATGTATGTTCTTTTCTTTGA+TGG + chr4.2:56230587-56230606 MS.gene064181:intron 25.0%
!! ATTTATGAATTGGCTTAACT+TGG - chr4.2:56232496-56232515 None:intergenic 25.0%
!! CGATTGAAAAATGATAATGT+TGG - chr4.2:56233117-56233136 None:intergenic 25.0%
!!! GGCTATTAGTTTTATAGATA+TGG + chr4.2:56233230-56233249 MS.gene064181:intron 25.0%
!!! TTGGTAATAGTAAGTTTTTC+AGG + chr4.2:56233493-56233512 MS.gene064181:intron 25.0%
!!! TTTTTTATCAGCTTTGTGTT+TGG + chr4.2:56230227-56230246 MS.gene064181:intron 25.0%
AAAACTATCTTGTAAGGTGA+GGG + chr4.2:56232384-56232403 MS.gene064181:intron 30.0%
AAAATGAGTTTATAAGTGGG+AGG - chr4.2:56232209-56232228 None:intergenic 30.0%
AAAATGTTCTTGAGCAAGAT+TGG - chr4.2:56232890-56232909 None:intergenic 30.0%
AAGTACATAAAGATGCTCTT+TGG - chr4.2:56232110-56232129 None:intergenic 30.0%
ACTTATAAAGCTCCTATCAT+TGG - chr4.2:56231477-56231496 None:intergenic 30.0%
ATGAAAGTGAAACTAACAAG+TGG - chr4.2:56231384-56231403 None:intergenic 30.0%
ATTTGTCTCAATGAACTCTT+TGG + chr4.2:56230288-56230307 MS.gene064181:intron 30.0%
CAAAGGATGTAAGCATAAAT+TGG - chr4.2:56232992-56233011 None:intergenic 30.0%
CAATTTATGCTTACATCCTT+TGG + chr4.2:56232990-56233009 MS.gene064181:intron 30.0%
CATAGTGACTGAAAATTAAG+TGG - chr4.2:56230677-56230696 None:intergenic 30.0%
CGTGAAAATGAGTTTATAAG+TGG - chr4.2:56232213-56232232 None:intergenic 30.0%
CTATATCTTTAGATCCTACA+CGG - chr4.2:56232544-56232563 None:intergenic 30.0%
CTGGTTAAACTATTCCAATA+TGG - chr4.2:56232829-56232848 None:intergenic 30.0%
GAATAAACGTACTTCTCATA+TGG - chr4.2:56231977-56231996 None:intergenic 30.0%
GACATATATTTAGAAGCTCT+TGG + chr4.2:56233474-56233493 MS.gene064181:intron 30.0%
GGAAATGGAAGAATAATATC+TGG + chr4.2:56231708-56231727 MS.gene064181:CDS 30.0%
GTAATGTCATGTTTATATGG+CGG + chr4.2:56230841-56230860 MS.gene064181:intron 30.0%
TAAGCATAAATTGGTGCATT+AGG - chr4.2:56232983-56233002 None:intergenic 30.0%
TAGTACAAGTGCAATTATGT+AGG - chr4.2:56233389-56233408 None:intergenic 30.0%
TCATTAGCATGATTAGAAAG+AGG - chr4.2:56230320-56230339 None:intergenic 30.0%
TGACTAATTATTGAATGTGG+TGG + chr4.2:56231340-56231359 MS.gene064181:intron 30.0%
TTGTGTACACTTCTGATTAA+AGG + chr4.2:56230008-56230027 MS.gene064181:intron 30.0%
TTTCTGTTCAACTACTGAAT+GGG + chr4.2:56233282-56233301 MS.gene064181:intron 30.0%
! AAATCCATTCATGTGCTTTT+AGG - chr4.2:56232959-56232978 None:intergenic 30.0%
! AACGAGAGTACAATATGAAA+TGG - chr4.2:56233031-56233050 None:intergenic 30.0%
! GCAAGTTATGCTTTTAATGT+TGG + chr4.2:56232031-56232050 MS.gene064181:intron 30.0%
! GGTTGATACTTGATAGTATA+TGG + chr4.2:56230169-56230188 MS.gene064181:intron 30.0%
! TGACTTTGAAGAAATATCCT+AGG - chr4.2:56230490-56230509 None:intergenic 30.0%
! TTAAATTATGAAGGTATGGC+AGG - chr4.2:56231158-56231177 None:intergenic 30.0%
! TTGAGTTTTTCTTTTCACCA+TGG - chr4.2:56231537-56231556 None:intergenic 30.0%
! TTTTCATTTTCAAACCGTGT+AGG + chr4.2:56232527-56232546 MS.gene064181:intron 30.0%
! TTTTCTGTTCAACTACTGAA+TGG + chr4.2:56233281-56233300 MS.gene064181:intron 30.0%
!! TTGAATGTGGTGGATAATTT+AGG + chr4.2:56231350-56231369 MS.gene064181:intron 30.0%
!!! ATGTTTATATGGCGGATTTT+TGG + chr4.2:56230849-56230868 MS.gene064181:intron 30.0%
AAACATGACATTACAACGGA+TGG - chr4.2:56230835-56230854 None:intergenic 35.0%
AAATTCTCATTTGTCAGCAG+AGG - chr4.2:56230992-56231011 None:intergenic 35.0%
AACAAAGTTCATTCGAAGCA+CGG - chr4.2:56232083-56232102 None:intergenic 35.0%
AATATCCTAGGAGCTTACTA+CGG - chr4.2:56230478-56230497 None:intergenic 35.0%
AATATGTTGAAGTCACGTGT+GGG + chr4.2:56229824-56229843 MS.gene064181:CDS 35.0%
AATGGTGATAAGGTTCCATT+GGG + chr4.2:56231740-56231759 MS.gene064181:CDS 35.0%
ACAGTATCTGTTGATGCAAA+AGG + chr4.2:56230959-56230978 MS.gene064181:intron 35.0%
ACTAAGTCTTGGTTAGAGTA+CGG + chr4.2:56232795-56232814 MS.gene064181:intron 35.0%
ATTATTTGTGAATGCACTCC+TGG + chr4.2:56229913-56229932 MS.gene064181:CDS 35.0%
CAAAACTATCTTGTAAGGTG+AGG + chr4.2:56232383-56232402 MS.gene064181:intron 35.0%
GCAATTATGTAGGTTATGTC+AGG - chr4.2:56233379-56233398 None:intergenic 35.0%
GCTCAAATACTACAATCAAG+TGG + chr4.2:56231775-56231794 MS.gene064181:CDS 35.0%
GGAAGATATCTAATTCCTTG+AGG - chr4.2:56233358-56233377 None:intergenic 35.0%
GGACGATATAATAGATGTTG+CGG - chr4.2:56230802-56230821 None:intergenic 35.0%
GGGAAGAGATCATATGATTA+AGG + chr4.2:56231197-56231216 MS.gene064181:intron 35.0%
GGTTCCAAAACTATCTTGTA+AGG + chr4.2:56232378-56232397 MS.gene064181:intron 35.0%
GTACGGTTTATGTTCCATAT+TGG + chr4.2:56232812-56232831 MS.gene064181:intron 35.0%
TAATGGTGATAAGGTTCCAT+TGG + chr4.2:56231739-56231758 MS.gene064181:CDS 35.0%
TCTCACGTTGGATAATGTAT+TGG - chr4.2:56232440-56232459 None:intergenic 35.0%
TGGTAAAGTCAGTACAAGTT+TGG - chr4.2:56233653-56233672 None:intergenic 35.0%
TTCTCTGTGTGTACTTTGTT+AGG + chr4.2:56232923-56232942 MS.gene064181:intron 35.0%
TTTAAACTGGCAGTGTACTT+TGG + chr4.2:56231016-56231035 MS.gene064181:intron 35.0%
! AGACTTAGGGTTTTTCTTAG+TGG + chr4.2:56229880-56229899 MS.gene064181:CDS 35.0%
! ATAGCCTATCCATGATTTGT+TGG - chr4.2:56232330-56232349 None:intergenic 35.0%
! ATTTCGTCTGAGATCTAAAG+AGG + chr4.2:56231892-56231911 MS.gene064181:CDS 35.0%
! CTATCCTAAAAGCACATGAA+TGG + chr4.2:56232952-56232971 MS.gene064181:intron 35.0%
! CTCACCTTACAAGATAGTTT+TGG - chr4.2:56232385-56232404 None:intergenic 35.0%
!! AATTTTGTTTGCCACATTGC+AGG + chr4.2:56231040-56231059 MS.gene064181:intron 35.0%
!! ATTTCTAGAACTTCAGCTGT+TGG + chr4.2:56233065-56233084 MS.gene064181:intron 35.0%
!! CTGAAGTTCTAGAAATAAGC+TGG - chr4.2:56233061-56233080 None:intergenic 35.0%
!! GATAGTATATGGTTTGGTGA+AGG + chr4.2:56230180-56230199 MS.gene064181:intron 35.0%
!! TAGCCTATCCATGATTTGTT+GGG - chr4.2:56232329-56232348 None:intergenic 35.0%
!!! TCATGTGCTTTTAGGATAGT+TGG - chr4.2:56232951-56232970 None:intergenic 35.0%
AAATGATTTCTTGCTCCCTC+TGG + chr4.2:56231276-56231295 MS.gene064181:intron 40.0%
AAGAAAGTGATGAGAGAGAG+AGG - chr4.2:56229953-56229972 None:intergenic 40.0%
AAGAGCTGAGAGAAATGAGT+GGG + chr4.2:56229785-56229804 MS.gene064181:CDS 40.0%
AATGATTTCTTGCTCCCTCT+GGG + chr4.2:56231277-56231296 MS.gene064181:intron 40.0%
AATTTGGGCACATTTCCTCA+AGG + chr4.2:56233340-56233359 MS.gene064181:intron 40.0%
ACAACACTGCAGTAGATACA+TGG - chr4.2:56230727-56230746 None:intergenic 40.0%
ACAACGGATGGCAGAAAAAT+TGG - chr4.2:56230823-56230842 None:intergenic 40.0%
ACACACAGAGAATCACTAGA+GGG - chr4.2:56232915-56232934 None:intergenic 40.0%
AGAAAGTGATGAGAGAGAGA+GGG - chr4.2:56229952-56229971 None:intergenic 40.0%
ATCTGTTGATGCAAAAGGAC+CGG + chr4.2:56230964-56230983 MS.gene064181:intron 40.0%
CAAACCCAGAATTCAGTAAC+AGG - chr4.2:56232853-56232872 None:intergenic 40.0%
CAACGTGAGACTCTTAACAA+TGG + chr4.2:56232450-56232469 MS.gene064181:intron 40.0%
CAGACCTGTTACTGAATTCT+GGG + chr4.2:56232846-56232865 MS.gene064181:intron 40.0%
CATTGTTAAGAGTCTCACGT+TGG - chr4.2:56232452-56232471 None:intergenic 40.0%
CCATGGATACATAAAGCACA+TGG - chr4.2:56231520-56231539 None:intergenic 40.0%
CCATGTGCTTTATGTATCCA+TGG + chr4.2:56231517-56231536 MS.gene064181:intron 40.0%
CTCTGCGAATTAGTAAGGAT+TGG - chr4.2:56232570-56232589 None:intergenic 40.0%
GAATATGTTGAAGTCACGTG+TGG + chr4.2:56229823-56229842 MS.gene064181:CDS 40.0%
GATATGTGCTTCAAGTCCAA+AGG - chr4.2:56233009-56233028 None:intergenic 40.0%
GGCAGTTGCAATCTGAAAAA+CGG - chr4.2:56233632-56233651 None:intergenic 40.0%
GGGTGTACTAGTCATAGATA+TGG + chr4.2:56229844-56229863 MS.gene064181:CDS 40.0%
GTCTCACATTGGATGAGATA+TGG - chr4.2:56232236-56232255 None:intergenic 40.0%
GTGTGTTAAGAGTCTCACAT+TGG - chr4.2:56232247-56232266 None:intergenic 40.0%
GTGTTGTCAAATTTCTGCCA+TGG + chr4.2:56230740-56230759 MS.gene064181:intron 40.0%
TACACACAGAGAATCACTAG+AGG - chr4.2:56232916-56232935 None:intergenic 40.0%
TGGATCAGAGTTATCAACGA+TGG - chr4.2:56230886-56230905 None:intergenic 40.0%
TGTTCAACTACTGAATGGGA+AGG + chr4.2:56233286-56233305 MS.gene064181:intron 40.0%
! ATGAGTTTGTTCGCTGTACT+AGG + chr4.2:56231852-56231871 MS.gene064181:CDS 40.0%
! CATAGATATGGTGATGCTGT+TGG + chr4.2:56229856-56229875 MS.gene064181:CDS 40.0%
! TGATGCTGTTGGTAGACTTA+GGG + chr4.2:56229867-56229886 MS.gene064181:CDS 40.0%
!!! CCTTACAAGCTGGTTTTGTA+CGG - chr4.2:56232177-56232196 None:intergenic 40.0%
ACATCACTCACCTTACAAGC+TGG - chr4.2:56232187-56232206 None:intergenic 45.0%
AGAGCTGAGAGAAATGAGTG+GGG + chr4.2:56229786-56229805 MS.gene064181:CDS 45.0%
CAGCACTCTGCGAATTAGTA+AGG - chr4.2:56232575-56232594 None:intergenic 45.0%
CCAGAATTCAGTAACAGGTC+TGG - chr4.2:56232848-56232867 None:intergenic 45.0%
CCAGACCTGTTACTGAATTC+TGG + chr4.2:56232845-56232864 MS.gene064181:intron 45.0%
CCGTACAAAACCAGCTTGTA+AGG + chr4.2:56232174-56232193 MS.gene064181:intron 45.0%
CGAAGAGTTTACAGAGGATG+CGG + chr4.2:56233549-56233568 MS.gene064181:CDS 45.0%
CTGCTGTGTCAGATGCAAAT+TGG + chr4.2:56231936-56231955 MS.gene064181:CDS 45.0%
GAAGAGAGACAGCAAGGAAA+TGG + chr4.2:56231693-56231712 MS.gene064181:CDS 45.0%
GAAGAGCTGAGAGAAATGAG+TGG + chr4.2:56229784-56229803 MS.gene064181:CDS 45.0%
GAATGGGAAGGACCAACTTA+TGG + chr4.2:56233298-56233317 MS.gene064181:intron 45.0%
GAGTGTTTCTCAAATGCAGC+AGG - chr4.2:56231671-56231690 None:intergenic 45.0%
GCACTTCTTGTGTGTGCTTT+GGG + chr4.2:56233595-56233614 MS.gene064181:CDS 45.0%
GCTGCATTTGAGAAACACTC+TGG + chr4.2:56231671-56231690 MS.gene064181:CDS 45.0%
GGAAGTCGAAGAGTTTACAG+AGG + chr4.2:56233543-56233562 MS.gene064181:CDS 45.0%
GGAGAAAATGGAGAATGGGT+TGG + chr4.2:56229729-56229748 MS.gene064181:CDS 45.0%
TACAGCGAACAAACTCATCG+CGG - chr4.2:56231850-56231869 None:intergenic 45.0%
TCAGAGTTATCAACGATGGC+TGG - chr4.2:56230882-56230901 None:intergenic 45.0%
TGCACTTCTTGTGTGTGCTT+TGG + chr4.2:56233594-56233613 MS.gene064181:CDS 45.0%
TGGCCCAACAAATCATGGAT+AGG + chr4.2:56232323-56232342 MS.gene064181:intron 45.0%
TGGGTTGTCAATGTTTAGCG+AGG + chr4.2:56229762-56229781 MS.gene064181:CDS 45.0%
TGTAACCGTAGTAAGCTCCT+AGG + chr4.2:56230470-56230489 MS.gene064181:intron 45.0%
TTGTTTGCCACATTGCAGGA+TGG + chr4.2:56231044-56231063 MS.gene064181:intron 45.0%
! AGCAGCACTGTTTTACCCAA+TGG - chr4.2:56231758-56231777 None:intergenic 45.0%
! GTGATGCTGTTGGTAGACTT+AGG + chr4.2:56229866-56229885 MS.gene064181:CDS 45.0%
! TCATTTGTCAGCAGAGGTAC+CGG - chr4.2:56230986-56231005 None:intergenic 45.0%
! TGTGATGATGAAGAAGAGCG+AGG + chr4.2:56233522-56233541 MS.gene064181:CDS 45.0%
!! AACCTTTGCACGTTGGTGAT+CGG - chr4.2:56233575-56233594 None:intergenic 45.0%
!! AATGGAGAATGGGTTGGAGA+AGG + chr4.2:56229735-56229754 MS.gene064181:CDS 45.0%
!! AATGGGTTGGAGAAGGGAAA+TGG + chr4.2:56229742-56229761 MS.gene064181:CDS 45.0%
!! ACGCTCTAGATGTCTGGTTT+TGG - chr4.2:56232280-56232299 None:intergenic 45.0%
!! ATGGAGAATGGGTTGGAGAA+GGG + chr4.2:56229736-56229755 MS.gene064181:CDS 45.0%
!! ATGGGTTGGAGAAGGGAAAT+GGG + chr4.2:56229743-56229762 MS.gene064181:CDS 45.0%
!!! CGCTCTAGATGTCTGGTTTT+GGG - chr4.2:56232279-56232298 None:intergenic 45.0%
!!! CTGGTTTTGTACGGTGAGTT+AGG - chr4.2:56232168-56232187 None:intergenic 45.0%
ATAGATGTTGCGGCCTCTGA+CGG - chr4.2:56230792-56230811 None:intergenic 50.0%
CAAGTGGCGAAAGCTGCTAA+TGG + chr4.2:56231791-56231810 MS.gene064181:CDS 50.0%
CAGCAGCATCATGGTGAATG+CGG - chr4.2:56231922-56231941 None:intergenic 50.0%
CATCTGACACAGCAGCATCA+TGG - chr4.2:56231931-56231950 None:intergenic 50.0%
CTCAGAGATCCCAAAATGGC+CGG + chr4.2:56231816-56231835 MS.gene064181:CDS 50.0%
CTTGACACCATCCTGCAATG+TGG - chr4.2:56231054-56231073 None:intergenic 50.0%
GAAGTGCAACCTTTGCACGT+TGG - chr4.2:56233582-56233601 None:intergenic 50.0%
GACTTGGATGGTTTGATCGC+AGG + chr4.2:56232300-56232319 MS.gene064181:intron 50.0%
GGCTCTCAGAGATCCCAAAA+TGG + chr4.2:56231812-56231831 MS.gene064181:CDS 50.0%
TCAGAGATCCCAAAATGGCC+GGG + chr4.2:56231817-56231836 MS.gene064181:CDS 50.0%
TGCACTCCTGGTTGTGATGA+AGG + chr4.2:56229925-56229944 MS.gene064181:CDS 50.0%
TTGGATGGTTTGATCGCAGG+TGG + chr4.2:56232303-56232322 MS.gene064181:intron 50.0%
! ACTCTGGAAGAGAGACAGCA+AGG + chr4.2:56231687-56231706 MS.gene064181:CDS 50.0%
! CAAGTCACGCTCTAGATGTC+TGG - chr4.2:56232286-56232305 None:intergenic 50.0%
! CACATTGCAGGATGGTGTCA+AGG + chr4.2:56231052-56231071 MS.gene064181:intron 50.0%
! CAGACATCTAGAGCGTGACT+TGG + chr4.2:56232284-56232303 MS.gene064181:intron 50.0%
! CATCTAGAGCGTGACTTGGA+TGG + chr4.2:56232288-56232307 MS.gene064181:intron 50.0%
! GATTTTGTGCTAGCCGTCAG+AGG + chr4.2:56230776-56230795 MS.gene064181:intron 50.0%
!!! TATGGCGGATTTTTGGCCTG+CGG + chr4.2:56230856-56230875 MS.gene064181:intron 50.0%
AATCCACCATGACGCTGCCA+TGG - chr4.2:56230760-56230779 None:intergenic 55.0%
AGATCCCAAAATGGCCGGGT+TGG + chr4.2:56231821-56231840 MS.gene064181:CDS 55.0%
CCGCTCAAAACACTCCCAGA+GGG - chr4.2:56231294-56231313 None:intergenic 55.0%
GAGGGACCTTCATCACAACC+AGG - chr4.2:56229934-56229953 None:intergenic 55.0%
GCAGGTGGCCCAACAAATCA+TGG + chr4.2:56232318-56232337 MS.gene064181:intron 55.0%
TCCGCTCAAAACACTCCCAG+AGG - chr4.2:56231295-56231314 None:intergenic 55.0%
TTTCTGCCATGGCAGCGTCA+TGG + chr4.2:56230751-56230770 MS.gene064181:intron 55.0%
!! GTGACCAACCCGGCCATTTT+GGG - chr4.2:56231828-56231847 None:intergenic 55.0%
!!! CCCTCTGGGAGTGTTTTGAG+CGG + chr4.2:56231291-56231310 MS.gene064181:intron 55.0%
!!! GTCTGGTTTTGGGTGCGAGA+GGG - chr4.2:56232269-56232288 None:intergenic 55.0%
!!! TGTCTGGTTTTGGGTGCGAG+AGG - chr4.2:56232270-56232289 None:intergenic 55.0%
ACTCATCGCGGTGACCAACC+CGG - chr4.2:56231838-56231857 None:intergenic 60.0%
GGCCGATCACCAACGTGCAA+AGG + chr4.2:56233570-56233589 MS.gene064181:CDS 60.0%
! GGTGACCAACCCGGCCATTT+TGG - chr4.2:56231829-56231848 None:intergenic 60.0%
! TATCAACGATGGCTGGCCGC+AGG - chr4.2:56230875-56230894 None:intergenic 60.0%
CTGCCATGGCAGCGTCATGG+TGG + chr4.2:56230754-56230773 MS.gene064181:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 56229727 56233689 56229727 ID=MS.gene064181
chr4.2 mRNA 56229727 56233689 56229727 ID=MS.gene064181.t1;Parent=MS.gene064181
chr4.2 exon 56229727 56229946 56229727 ID=MS.gene064181.t1.exon1;Parent=MS.gene064181.t1
chr4.2 CDS 56229727 56229946 56229727 ID=cds.MS.gene064181.t1;Parent=MS.gene064181.t1
chr4.2 exon 56231657 56231984 56231657 ID=MS.gene064181.t1.exon2;Parent=MS.gene064181.t1
chr4.2 CDS 56231657 56231984 56231657 ID=cds.MS.gene064181.t1;Parent=MS.gene064181.t1
chr4.2 exon 56233503 56233689 56233503 ID=MS.gene064181.t1.exon3;Parent=MS.gene064181.t1
chr4.2 CDS 56233503 56233689 56233503 ID=cds.MS.gene064181.t1;Parent=MS.gene064181.t1
Gene Sequence

>MS.gene064181

ATGGAGAAAATGGAGAATGGGTTGGAGAAGGGAAATGGGTTGTCAATGTTTAGCGAGGAAGAGCTGAGAGAAATGAGTGGGGTTAGTGTTTTTGATGAATATGTTGAAGTCACGTGTGGGTGTACTAGTCATAGATATGGTGATGCTGTTGGTAGACTTAGGGTTTTTCTTAGTGGATACCTTCAAATTATTTGTGAATGCACTCCTGGTTGTGATGAAGGTCCCTCTCTCTCTCATCACTTTCTTTGTTTTTCTTGTTTATGTGCATGTTGATAGTTGTGTTGTGTACACTTCTGATTAAAGGCGTGTTCTGTATCCGACATGCATAAATACTTATATACAACACTGATACATGTGATTACATTGATTTTCTTACATTTTTTTGTTTTTATCTAAGTCGGCGTGTCAGTGTCGTGTCATGTGTATGTGTTAGTATTTCATAGGTTGATACTTGATAGTATATGGTTTGGTGAAGGTTTGACTTTGATAAGTTTTATTGTTTTTTTATCAGCTTTGTGTTTGGAGTTTGTGCTGATATATTGACATCTTAAGTGTATGTTGATTTGTCTCAATGAACTCTTTGGTTTGATCCTCTTTCTAATCATGCTAATGAATGTTTATTGTATGAAAATTTATAATTGGCATTCTGTCTTTTTGTGAGATTAAATTCAATATGGCTTGTATTTTTGTTAAATATTACTTTTTCTATATAGTTACTTTGTACGTATTTAATAATAACATAATGTAACCGTAGTAAGCTCCTAGGATATTTCTTCAAAGTCATTGAACTTTGCAAAGTAGAGTGTAGATTGTATAGTAATTAATATCCTAAGGTATTGTTAATATATAACCTACAAGTTATGTATGTTCTTTTCTTTGATGGCACATTCTCTTATTTTATGTGTGAATGTTATGATAGCTATTTGTATTGCAGCCTCATATTCATACCACTTAATTTTCAGTCACTATGCACATGCAATGTCAAGTTGTACTTATTCCATGTATCTACTGCAGTGTTGTCAAATTTCTGCCATGGCAGCGTCATGGTGGATTTTGTGCTAGCCGTCAGAGGCCGCAACATCTATTATATCGTCCAATTTTTCTGCCATCCGTTGTAATGTCATGTTTATATGGCGGATTTTTGGCCTGCGGCCAGCCATCGTTGATAACTCTGATCCACTATGTGTGTTGTTTTATTACTTGAACATTGATATATACAAGTAGAACTTGGTACAGTATCTGTTGATGCAAAAGGACCGGTACCTCTGCTGACAAATGAGAATTTGTGATTTAAACTGGCAGTGTACTTTGGAAATTTTGTTTGCCACATTGCAGGATGGTGTCAAGGCACTAAATGACATGTGTTTAAGTAATTATGTCAACACTTTATACATGAAATATACTGACTTAAGAAAATATGAATTATATCCTGCCATACCTTCATAATTTAATTTGAAAAATCAACAACTTGGGAAGAGATCATATGATTAAGGTTTGATGAAAATTCTAAACTTTTACTCCTAAAAATATAAATAAAAGAAGATAAGAGAAATGATTTCTTGCTCCCTCTGGGAGTGTTTTGAGCGGATGAGCACAAGTTTATGCCGTCTATATGACTAATTATTGAATGTGGTGGATAATTTAGGTAAACGATCCACTTGTTAGTTTCACTTTCATTTTATTAGATGATTTTAATAATTTCAGCCAATAATTTATGATTTTGTTTTGGTCATAGTCATTCATATATCCAATGATAGGAGCTTTATAAGTATGCCATTGTATATTTTTTTCCATGTGCTTTATGTATCCATGGTGAAAAGAAAAACTCAATTTCTGATTGTGATTCTCTATCTTACATATAAGTAGTGTTAACGGCATTGTTTTTCTCGTTCACGACCTTAATTTTTGCATATGATGTTTTAAATGATAGACAAGTTGACACCTGCTGCATTTGAGAAACACTCTGGAAGAGAGACAGCAAGGAAATGGAAGAATAATATCTGGGTTATAATTAATGGTGATAAGGTTCCATTGGGTAAAACAGTGCTGCTCAAATACTACAATCAAGTGGCGAAAGCTGCTAATGGCTCTCAGAGATCCCAAAATGGCCGGGTTGGTCACCGCGATGAGTTTGTTCGCTGTACTAGGTGCAACAAAGAGCGTAGATTTCGTCTGAGATCTAAAGAGGAATGCCGCATTCACCATGATGCTGCTGTGTCAGATGCAAATTGGAAATGTTCTGATCTTCCATATGAGAAGTACGTTTATTCATCTACACTTTCACTAAGAATAAAAAATAAGTCAGCAAGTTATGCTTTTAATGTTGGTGACTTGTGTTTAATAATTTCTGTCACCGTGCTTCGAATGAACTTTGTTTGTACCAAAGAGCATCTTTATGTACTTTTTTTCTATTGATAGATCTTAGAATTTGTGTTGGGCCTAACTCACCGTACAAAACCAGCTTGTAAGGTGAGTGATGTCTCCCACTTATAAACTCATTTTCACGCCATATCTCATCCAATGTGAGACTCTTAACACACCCTCTCGCACCCAAAACCAGACATCTAGAGCGTGACTTGGATGGTTTGATCGCAGGTGGCCCAACAAATCATGGATAGGCTATAATATATATAATCTTTGAATTTGGGTTGGGTTCCAAAACTATCTTGTAAGGTGAGGGATGCCTCCCACTTATAAACTCATTTTCAGACCAATACATTATCCAACGTGAGACTCTTAACAATGGAAATAGTGCACAACTGCTTTCCAAGTTAAGCCAATTCATAAATTTGGTCTTTTCTTTTCATTTTCAAACCGTGTAGGATCTAAAGATATAGTCACCAATCCTTACTAATTCGCAGAGTGCTGTACAAATAATTGATAGTCTTAATCTAGGATATCTTATATTTTTTACTCTATATCTGACTACAGACTGTTAAACTATATATGTACTCTTAGAACTACCGAACTATGATAAATAACTGCTAAATCTTTTGCATAAAATTTCAGGATGAAATATATTTTATTGGTATTTTGTTATTGGTATATGCTAATTAATTCTTAAAGTTACTAAGTCTTGGTTAGAGTACGGTTTATGTTCCATATTGGAATAGTTTAACCAGACCTGTTACTGAATTCTGGGTTTGTCTCAATGAAACAGCCAATCTTGCTCAAGAACATTTTGTCCCTCTAGTGATTCTCTGTGTGTACTTTGTTAGGAACCAACTATCCTAAAAGCACATGAATGGATTTACCTAATGCACCAATTTATGCTTACATCCTTTGGACTTGAAGCACATATCCATTTCATATTGTACTCTCGTTTCTCAAACCAGCTTATTTCTAGAACTTCAGCTGTTGGATGAAAACACAAAATGTTTTTAATATCCAACATTATCATTTTTCAATCGTTTAACTGTTCTGCTTTTACATTGTCAGTACTAGCTTAATTTCAAAATGTGTATATCTCTATTTTTTTTTATTAATTTTTTGACCAAATTTAAGGCTATTAGTTTTATAGATATGGTTATCATTTGGAACTTGTGTTGCCTTTATTTTCTGTTCAACTACTGAATGGGAAGGACCAACTTATGGTTTATTATAAAAGACTCAGAATTTGGGCACATTTCCTCAAGGAATTAGATATCTTCCTGACATAACCTACATAATTGCACTTGTACTATTTATTTAGGCACATGTGTGCTCTATTATCAGTTGTGTAGTCACCTTAATTATTACCATAAAAATGACATATATTTAGAAGCTCTTGGTAATAGTAAGTTTTTCAGGATAACATGTGATGATGAAGAAGAGCGAGGAAGTCGAAGAGTTTACAGAGGATGCGGCCGATCACCAACGTGCAAAGGTTGCACTTCTTGTGTGTGCTTTGGGTGTGACATCTGCCGTTTTTCAGATTGCAACTGCCAAACTTGTACTGACTTTACCAGCAATGTAAAAGCTTGA
Protein sequence

>MS.gene064181.t1

MEKMENGLEKGNGLSMFSEEELREMSGVSVFDEYVEVTCGCTSHRYGDAVGRLRVFLSGYLQIICECTPGCDEDKLTPAAFEKHSGRETARKWKNNIWVIINGDKVPLGKTVLLKYYNQVAKAANGSQRSQNGRVGHRDEFVRCTRCNKERRFRLRSKEECRIHHDAAVSDANWKCSDLPYENKFFRITCDDEEERGSRRVYRGCGRSPTCKGCTSCVCFGCDICRFSDCNCQTCTDFTSNVKA