AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064258

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064258.t1 MTR_7g080620 88.642 405 22 1 37 417 122 526 0.0 729
MS.gene064258.t1 MTR_8g005155 52.985 402 162 3 40 417 108 506 5.14e-144 422
MS.gene064258.t1 MTR_7g093000 20.286 350 259 7 40 381 118 455 1.17e-13 73.2
MS.gene064258.t1 MTR_5g084810 20.563 355 262 6 41 391 142 480 1.54e-12 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064258.t1 AT3G60400 45.297 404 189 3 40 417 101 498 2.16e-108 330
MS.gene064258.t1 AT4G19650 22.817 355 245 7 40 386 151 484 3.01e-17 84.3
MS.gene064258.t1 AT4G19650 22.817 355 245 7 40 386 134 467 3.58e-17 84.0
MS.gene064258.t1 AT5G06811 24.069 403 258 11 41 412 136 521 1.45e-13 72.8

Find 87 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTGTGTGCTTTGTGATTT+TGG 0.070594 7.4:-22290092 MS.gene064258:CDS
TGCATTGACTATGAATCTTT+TGG 0.189736 7.4:-22289241 MS.gene064258:CDS
TTTGGTAATGTTGAAGTTAT+TGG 0.228613 7.4:-22289963 MS.gene064258:CDS
GAGGATGTTATTGAAAATTT+TGG 0.244503 7.4:-22289513 MS.gene064258:CDS
ATGGAGAAGAGTTCTATATT+TGG 0.292885 7.4:-22290038 MS.gene064258:CDS
TTGGTAATGTTGAAGTTATT+GGG 0.297592 7.4:-22289962 MS.gene064258:CDS
GTAGAGTATTTCTGTGGATT+TGG 0.306808 7.4:-22289663 MS.gene064258:CDS
CTTTGTTGGTTTAAAAGATT+TGG 0.309206 7.4:-22289987 MS.gene064258:CDS
TGAAGTGTTAAAGCTTGATT+TGG 0.312206 7.4:-22289598 MS.gene064258:CDS
GAAGTTAGGTGTGTTGTATA+TGG 0.320179 7.4:-22290057 MS.gene064258:CDS
TGGCATGAGCTCTAAAGATT+TGG 0.330860 7.4:-22289535 MS.gene064258:CDS
GGGTTTCCTTGGGAGAAGTT+AGG 0.333161 7.4:-22290071 MS.gene064258:CDS
CGGCACAAGCAGTGAGTTAC+AGG 0.339180 7.4:-22289208 MS.gene064258:CDS
TGGAGAAGAGTTCTATATTT+GGG 0.347223 7.4:-22290037 MS.gene064258:CDS
ATGGTTCTGTGATAAGTTAA+AGG 0.357332 7.4:-22289424 MS.gene064258:CDS
GAGCTATGGGTTTGCAAGAA+TGG 0.361647 7.4:-22289443 MS.gene064258:CDS
ATTCAATTCTTGAGCGTAGA+AGG 0.364335 7.4:+22289172 None:intergenic
AGTTAAAGGGTGGGCATTAT+CGG 0.373850 7.4:-22289410 MS.gene064258:CDS
AAGTGATAATGCAACATTGC+TGG 0.384343 7.4:+22289021 None:intergenic
GGGAACAACTTTGGACTGTC+TGG 0.390764 7.4:-22289052 MS.gene064258:CDS
TTCTTACATAGATTAGGCTT+TGG 0.390843 7.4:-22289270 MS.gene064258:CDS
AGTTGAAATTGAGGCTTTGT+TGG 0.393745 7.4:-22290001 MS.gene064258:CDS
TTAAATTTCTTACATAGATT+AGG 0.396832 7.4:-22289276 MS.gene064258:CDS
TGAAGTTATTGGGATTTGTT+TGG 0.398881 7.4:-22289952 MS.gene064258:CDS
CTGTGGATTTGGTGGAAAGA+AGG 0.405320 7.4:-22289652 MS.gene064258:CDS
ATCTGTACCTAGGTATAATT+CGG 0.410370 7.4:+22288992 None:intergenic
CCCATAGCTCTCATCACATT+TGG 0.415076 7.4:+22289456 None:intergenic
TTTGGTGTTGTGGAGGTAAT+CGG 0.422094 7.4:+22290361 None:intergenic
CCAGGAAATGGGAACAACTT+TGG 0.424489 7.4:-22289061 MS.gene064258:intron
AAATCAAGCTTTAACACTTC+AGG 0.444612 7.4:+22289600 None:intergenic
CATGAGCTCTAAAGATTTGG+AGG 0.452449 7.4:-22289532 MS.gene064258:CDS
TGGTTTAAAAGATTTGGTTT+TGG 0.453368 7.4:-22289981 MS.gene064258:CDS
AAGATTATCAAGATGGTTTG+AGG 0.456096 7.4:-22289341 MS.gene064258:CDS
CATAGGGATAGATTATACTT+TGG 0.460683 7.4:-22290177 MS.gene064258:CDS
TTGAGGGAAATGTAGATTCA+TGG 0.468819 7.4:-22289812 MS.gene064258:CDS
TGGTGGAAAGAAGGAGGAAG+TGG 0.471562 7.4:-22289643 MS.gene064258:CDS
TCTTTGGGGAGTTATGTTGA+GGG 0.476597 7.4:-22289828 MS.gene064258:CDS
GAGTATTTCTGTGGATTTGG+TGG 0.478331 7.4:-22289660 MS.gene064258:CDS
CGGGATAAAGATTATCAAGA+TGG 0.492595 7.4:-22289348 MS.gene064258:CDS
ATAGGTGAAGGGTATGGATT+TGG 0.493429 7.4:+22290343 None:intergenic
AATGGATGGAAGGGTAATGT+AGG 0.497129 7.4:-22289750 MS.gene064258:CDS
GAAAAGAACAGCTTATCATT+CGG 0.501059 7.4:+22290140 None:intergenic
GGAAGTGGGTCAAATTCAAA+AGG 0.502040 7.4:-22289907 MS.gene064258:CDS
TGGTTCTGTGATAAGTTAAA+GGG 0.503197 7.4:-22289423 MS.gene064258:CDS
CAAGTGATCGCAATGAAGAC+CGG 0.503592 7.4:-22289368 MS.gene064258:CDS
TTCTTTGGGGAGTTATGTTG+AGG 0.504050 7.4:-22289829 MS.gene064258:CDS
ACTTCCTCACTCAAAACAAA+AGG 0.505183 7.4:+22289924 None:intergenic
CATCTTGATAATCTTTATCC+CGG 0.516234 7.4:+22289349 None:intergenic
AGCAATGTTGCATTATCACT+TGG 0.516606 7.4:-22289019 MS.gene064258:CDS
CCAAATGTGATGAGAGCTAT+GGG 0.525151 7.4:-22289456 MS.gene064258:CDS
ACCAAATGTGATGAGAGCTA+TGG 0.530073 7.4:-22289457 MS.gene064258:CDS
TACGATTTGAATGGATGGAA+GGG 0.534342 7.4:-22289759 MS.gene064258:CDS
TTACGATTTGAATGGATGGA+AGG 0.534900 7.4:-22289760 MS.gene064258:CDS
TGTGTTGGGAACAATTAGAA+TGG 0.537589 7.4:-22289487 MS.gene064258:CDS
CCTCACAAATCTCATTTCAA+AGG 0.541683 7.4:-22290288 MS.gene064258:intron
AGGGTATGGATTTGGTGTTG+TGG 0.544585 7.4:+22290351 None:intergenic
GTGTTGAAGAGTTGAAATTG+AGG 0.549592 7.4:-22290010 MS.gene064258:CDS
GTTGCCAATGTGTTCTGCAT+AGG 0.550077 7.4:+22290325 None:intergenic
TTGGAGCTGTTGAAACACTT+TGG 0.555886 7.4:-22289555 MS.gene064258:CDS
ATTCTCAAAGCTCTGCATGA+TGG 0.566277 7.4:-22289151 MS.gene064258:intron
GGGTAATGTAGGAGAACTCA+TGG 0.567143 7.4:-22289739 MS.gene064258:CDS
AAAGCTTGATTTGGAGAAGA+CGG 0.572369 7.4:-22289589 MS.gene064258:CDS
CTTTGAAATGAGATTTGTGA+GGG 0.572972 7.4:+22290289 None:intergenic
CATAAAGTAGAGTATTTCTG+TGG 0.577594 7.4:-22289669 MS.gene064258:CDS
TTCTGTGATAAGTTAAAGGG+TGG 0.581828 7.4:-22289420 MS.gene064258:CDS
TGGATTTGGTGGAAAGAAGG+AGG 0.582914 7.4:-22289649 MS.gene064258:CDS
ATTTGTTGATCACAAAGAAG+AGG 0.582958 7.4:-22289700 MS.gene064258:CDS
TTTGAGCTTCGTGAATGACA+TGG 0.585863 7.4:+22290393 None:intergenic
GAAGACGGTGATTAATGTGT+TGG 0.586071 7.4:-22289574 MS.gene064258:CDS
AAGTGATCGCAATGAAGACC+GGG 0.587085 7.4:-22289367 MS.gene064258:CDS
GGTAATGTAGGAGAACTCAT+GGG 0.593941 7.4:-22289738 MS.gene064258:CDS
GTTGTGGAGGTAATCGGTGA+GGG 0.598909 7.4:+22290367 None:intergenic
TTCTGCATAGGTGAAGGGTA+TGG 0.599546 7.4:+22290337 None:intergenic
ATGTGTTCTGCATAGGTGAA+GGG 0.618433 7.4:+22290332 None:intergenic
AACACACCTAACTTCTCCCA+AGG 0.625967 7.4:+22290065 None:intergenic
GTATGGATTTGGTGTTGTGG+AGG 0.635398 7.4:+22290354 None:intergenic
GAAGTGGGTCAAATTCAAAA+GGG 0.636084 7.4:-22289906 MS.gene064258:CDS
AAATGTAGATTCATGGCATG+AGG 0.639289 7.4:-22289805 MS.gene064258:CDS
TGGGCATTATCGGCTGCTAG+CGG 0.639965 7.4:-22289400 MS.gene064258:CDS
TTCACCTATGCAGAACACAT+TGG 0.649247 7.4:-22290329 MS.gene064258:CDS
TGTTGTGGAGGTAATCGGTG+AGG 0.650161 7.4:+22290366 None:intergenic
AATGTGTTCTGCATAGGTGA+AGG 0.659720 7.4:+22290331 None:intergenic
TGGATGACCGAATTATACCT+AGG 0.659803 7.4:-22288999 None:intergenic
GTAATGTAGGAGAACTCATG+GGG 0.661705 7.4:-22289737 MS.gene064258:CDS
TCTGTGATAAGTTAAAGGGT+GGG 0.664345 7.4:-22289419 MS.gene064258:CDS
ATGTAGGAGAACTCATGGGG+AGG 0.683085 7.4:-22289734 MS.gene064258:CDS
CCTTTGAAATGAGATTTGTG+AGG 0.730568 7.4:+22290288 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATTTCTTACATAGATT+AGG - chr7.4:22290115-22290134 MS.gene064258:CDS 15.0%
!! ATAACATTAAAAGAGTCATT+AGG - chr7.4:22289153-22289172 MS.gene064258:CDS 20.0%
!!! AATTTTCTTGGATAATTCTT+TGG - chr7.4:22289547-22289566 MS.gene064258:CDS 20.0%
!!! ATTTTCTTGGATAATTCTTT+GGG - chr7.4:22289548-22289567 MS.gene064258:CDS 20.0%
!!! GCTCTATAATTTCATAATTT+TGG + chr7.4:22290291-22290310 None:intergenic 20.0%
!!! TGATCTAAAGTTAATTTTCT+TGG - chr7.4:22289535-22289554 MS.gene064258:CDS 20.0%
!!! TTAATGTTATTTGAGAAAGT+GGG + chr7.4:22289143-22289162 None:intergenic 20.0%
!!! TTTAATGTTATTTGAGAAAG+TGG + chr7.4:22289144-22289163 None:intergenic 20.0%
! AAAGACTTCAAATTCGTTAA+TGG + chr7.4:22289186-22289205 None:intergenic 25.0%
! AAGACTTCAAATTCGTTAAT+GGG + chr7.4:22289185-22289204 None:intergenic 25.0%
!! GAGGATGTTATTGAAAATTT+TGG - chr7.4:22289878-22289897 MS.gene064258:CDS 25.0%
!! TTTTCTTGGATAATTCTTTG+GGG - chr7.4:22289549-22289568 MS.gene064258:CDS 25.0%
!!! AAAAGTGAATTTTTTCAACC+AGG - chr7.4:22290312-22290331 MS.gene064258:CDS 25.0%
!!! AAGTCTTTTTTGAAAGCATA+GGG - chr7.4:22289198-22289217 MS.gene064258:CDS 25.0%
!!! AATTTTTTCAACCAGGAAAT+GGG - chr7.4:22290319-22290338 MS.gene064258:CDS 25.0%
!!! CGATTTTTTTACGATTTGAA+TGG - chr7.4:22289623-22289642 MS.gene064258:CDS 25.0%
!!! CTTTGTTGGTTTAAAAGATT+TGG - chr7.4:22289404-22289423 MS.gene064258:CDS 25.0%
!!! TGGTTTAAAAGATTTGGTTT+TGG - chr7.4:22289410-22289429 MS.gene064258:CDS 25.0%
!!! TTGGTAATGTTGAAGTTATT+GGG - chr7.4:22289429-22289448 MS.gene064258:CDS 25.0%
!!! TTTGGTAATGTTGAAGTTAT+TGG - chr7.4:22289428-22289447 MS.gene064258:CDS 25.0%
!!! TTTTTTACGATTTGAATGGA+TGG - chr7.4:22289627-22289646 MS.gene064258:CDS 25.0%
AAGATTATCAAGATGGTTTG+AGG - chr7.4:22290050-22290069 MS.gene064258:CDS 30.0%
AGACTTCAAATTCGTTAATG+GGG + chr7.4:22289184-22289203 None:intergenic 30.0%
ATGGAGAAGAGTTCTATATT+TGG - chr7.4:22289353-22289372 MS.gene064258:CDS 30.0%
CATAGGGATAGATTATACTT+TGG - chr7.4:22289214-22289233 MS.gene064258:CDS 30.0%
GAAAAGAACAGCTTATCATT+CGG + chr7.4:22289254-22289273 None:intergenic 30.0%
TGGAGAAGAGTTCTATATTT+GGG - chr7.4:22289354-22289373 MS.gene064258:CDS 30.0%
TTCTTACATAGATTAGGCTT+TGG - chr7.4:22290121-22290140 MS.gene064258:CDS 30.0%
! AAATCAAGCTTTAACACTTC+AGG + chr7.4:22289794-22289813 None:intergenic 30.0%
! ATGGTTCTGTGATAAGTTAA+AGG - chr7.4:22289967-22289986 MS.gene064258:CDS 30.0%
! ATTTGTTGATCACAAAGAAG+AGG - chr7.4:22289691-22289710 MS.gene064258:CDS 30.0%
! CATAAAGTAGAGTATTTCTG+TGG - chr7.4:22289722-22289741 MS.gene064258:CDS 30.0%
! CATCTTGATAATCTTTATCC+CGG + chr7.4:22290045-22290064 None:intergenic 30.0%
! TGAAGTGTTAAAGCTTGATT+TGG - chr7.4:22289793-22289812 MS.gene064258:CDS 30.0%
! TGAAGTTATTGGGATTTGTT+TGG - chr7.4:22289439-22289458 MS.gene064258:CDS 30.0%
! TGGTTCTGTGATAAGTTAAA+GGG - chr7.4:22289968-22289987 MS.gene064258:CDS 30.0%
!! CTTTGAAATGAGATTTGTGA+GGG + chr7.4:22289105-22289124 None:intergenic 30.0%
!! GAAAATTTTGGTCATGTGTT+GGG - chr7.4:22289890-22289909 MS.gene064258:CDS 30.0%
!! TGAAAATTTTGGTCATGTGT+TGG - chr7.4:22289889-22289908 MS.gene064258:CDS 30.0%
!!! AATTTTGGTTCAGAATCCTT+GGG + chr7.4:22290276-22290295 None:intergenic 30.0%
!!! CAAATTCAAAAGGGTGTTTT+TGG - chr7.4:22289494-22289513 MS.gene064258:CDS 30.0%
!!! GAAGTCTTTTTTGAAAGCAT+AGG - chr7.4:22289197-22289216 MS.gene064258:CDS 30.0%
!!! GAATTTTTTCAACCAGGAAA+TGG - chr7.4:22290318-22290337 MS.gene064258:CDS 30.0%
!!! TAATTTTGGTTCAGAATCCT+TGG + chr7.4:22290277-22290296 None:intergenic 30.0%
!!! TGCATTGACTATGAATCTTT+TGG - chr7.4:22290150-22290169 MS.gene064258:CDS 30.0%
!!! TTTTCCTTTTGTTTTGAGTG+AGG - chr7.4:22289463-22289482 MS.gene064258:CDS 30.0%
AAATGTAGATTCATGGCATG+AGG - chr7.4:22289586-22289605 MS.gene064258:CDS 35.0%
ACTTCCTCACTCAAAACAAA+AGG + chr7.4:22289470-22289489 None:intergenic 35.0%
AGCAATGTTGCATTATCACT+TGG - chr7.4:22290372-22290391 MS.gene064258:CDS 35.0%
ATTCAATTCTTGAGCGTAGA+AGG + chr7.4:22290222-22290241 None:intergenic 35.0%
CCTCACAAATCTCATTTCAA+AGG - chr7.4:22289103-22289122 MS.gene064258:intron 35.0%
CGGGATAAAGATTATCAAGA+TGG - chr7.4:22290043-22290062 MS.gene064258:CDS 35.0%
GAAGTGGGTCAAATTCAAAA+GGG - chr7.4:22289485-22289504 MS.gene064258:CDS 35.0%
GAAGTTAGGTGTGTTGTATA+TGG - chr7.4:22289334-22289353 MS.gene064258:CDS 35.0%
GTGTTGAAGAGTTGAAATTG+AGG - chr7.4:22289381-22289400 MS.gene064258:CDS 35.0%
TACGATTTGAATGGATGGAA+GGG - chr7.4:22289632-22289651 MS.gene064258:CDS 35.0%
TCTGTGATAAGTTAAAGGGT+GGG - chr7.4:22289972-22289991 MS.gene064258:CDS 35.0%
TGTGTTGGGAACAATTAGAA+TGG - chr7.4:22289904-22289923 MS.gene064258:CDS 35.0%
TTACGATTTGAATGGATGGA+AGG - chr7.4:22289631-22289650 MS.gene064258:CDS 35.0%
TTCTGTGATAAGTTAAAGGG+TGG - chr7.4:22289971-22289990 MS.gene064258:CDS 35.0%
TTGAGGGAAATGTAGATTCA+TGG - chr7.4:22289579-22289598 MS.gene064258:CDS 35.0%
! AAGTGATAATGCAACATTGC+TGG + chr7.4:22290373-22290392 None:intergenic 35.0%
! AATCTTTTGGATCGTTTGCA+CGG - chr7.4:22290163-22290182 MS.gene064258:CDS 35.0%
! AGTTGAAATTGAGGCTTTGT+TGG - chr7.4:22289390-22289409 MS.gene064258:CDS 35.0%
! CTTGTGTGCTTTGTGATTTT+GGG - chr7.4:22289300-22289319 MS.gene064258:CDS 35.0%
! GTAGAGTATTTCTGTGGATT+TGG - chr7.4:22289728-22289747 MS.gene064258:CDS 35.0%
! TGATAAGCTGTTCTTTTCAG+AGG - chr7.4:22289256-22289275 MS.gene064258:CDS 35.0%
!! AAAGCTTGATTTGGAGAAGA+CGG - chr7.4:22289802-22289821 MS.gene064258:CDS 35.0%
!! CCTTTGAAATGAGATTTGTG+AGG + chr7.4:22289106-22289125 None:intergenic 35.0%
!!! ATTTTGGTTCAGAATCCTTG+GGG + chr7.4:22290275-22290294 None:intergenic 35.0%
!!! TTGTGATTTTGGGTTTCCTT+GGG - chr7.4:22289310-22289329 MS.gene064258:CDS 35.0%
!!! TTTGTGATTTTGGGTTTCCT+TGG - chr7.4:22289309-22289328 MS.gene064258:CDS 35.0%
!!! TTTGTTTTGAGTGAGGAAGT+GGG - chr7.4:22289470-22289489 MS.gene064258:CDS 35.0%
!!! TTTTGTTTTGAGTGAGGAAG+TGG - chr7.4:22289469-22289488 MS.gene064258:CDS 35.0%
AATGGATGGAAGGGTAATGT+AGG - chr7.4:22289641-22289660 MS.gene064258:CDS 40.0%
AATGTGTTCTGCATAGGTGA+AGG + chr7.4:22289063-22289082 None:intergenic 40.0%
ACCAAATGTGATGAGAGCTA+TGG - chr7.4:22289934-22289953 MS.gene064258:CDS 40.0%
AGTTAAAGGGTGGGCATTAT+CGG - chr7.4:22289981-22290000 MS.gene064258:CDS 40.0%
ATGTGTTCTGCATAGGTGAA+GGG + chr7.4:22289062-22289081 None:intergenic 40.0%
ATTCTCAAAGCTCTGCATGA+TGG - chr7.4:22290240-22290259 MS.gene064258:intron 40.0%
CCAAATGTGATGAGAGCTAT+GGG - chr7.4:22289935-22289954 MS.gene064258:CDS 40.0%
GAAGACGGTGATTAATGTGT+TGG - chr7.4:22289817-22289836 MS.gene064258:CDS 40.0%
GGAAGTGGGTCAAATTCAAA+AGG - chr7.4:22289484-22289503 MS.gene064258:CDS 40.0%
GGTAATGTAGGAGAACTCAT+GGG - chr7.4:22289653-22289672 MS.gene064258:CDS 40.0%
GTAATGTAGGAGAACTCATG+GGG - chr7.4:22289654-22289673 MS.gene064258:CDS 40.0%
TGATGGTAAGAAAAACCCCA+AGG - chr7.4:22290257-22290276 MS.gene064258:intron 40.0%
TTCACCTATGCAGAACACAT+TGG - chr7.4:22289062-22289081 MS.gene064258:intron 40.0%
TTGGAGCTGTTGAAACACTT+TGG - chr7.4:22289836-22289855 MS.gene064258:CDS 40.0%
! AGCTGTTCTTTTCAGAGGAT+AGG - chr7.4:22289261-22289280 MS.gene064258:CDS 40.0%
! ATAGGTGAAGGGTATGGATT+TGG + chr7.4:22289051-22289070 None:intergenic 40.0%
! GAGTATTTCTGTGGATTTGG+TGG - chr7.4:22289731-22289750 MS.gene064258:CDS 40.0%
! GCTTGTGTGCTTTGTGATTT+TGG - chr7.4:22289299-22289318 MS.gene064258:CDS 40.0%
! TCTTTGGGGAGTTATGTTGA+GGG - chr7.4:22289563-22289582 MS.gene064258:CDS 40.0%
! TTCTTTGGGGAGTTATGTTG+AGG - chr7.4:22289562-22289581 MS.gene064258:CDS 40.0%
!! CATGAGCTCTAAAGATTTGG+AGG - chr7.4:22289859-22289878 MS.gene064258:CDS 40.0%
!! TGGCATGAGCTCTAAAGATT+TGG - chr7.4:22289856-22289875 MS.gene064258:CDS 40.0%
!! TTTGGTGTTGTGGAGGTAAT+CGG + chr7.4:22289033-22289052 None:intergenic 40.0%
AACACACCTAACTTCTCCCA+AGG + chr7.4:22289329-22289348 None:intergenic 45.0%
AAGTGATCGCAATGAAGACC+GGG - chr7.4:22290024-22290043 MS.gene064258:CDS 45.0%
CAAGTGATCGCAATGAAGAC+CGG - chr7.4:22290023-22290042 MS.gene064258:CDS 45.0%
CCAAAGTTGTTCCCATTTCC+TGG + chr7.4:22290333-22290352 None:intergenic 45.0%
CCAGGAAATGGGAACAACTT+TGG - chr7.4:22290330-22290349 MS.gene064258:CDS 45.0%
CCCATAGCTCTCATCACATT+TGG + chr7.4:22289938-22289957 None:intergenic 45.0%
GAGCTATGGGTTTGCAAGAA+TGG - chr7.4:22289948-22289967 MS.gene064258:CDS 45.0%
GGGTAATGTAGGAGAACTCA+TGG - chr7.4:22289652-22289671 MS.gene064258:CDS 45.0%
GTTGCCAATGTGTTCTGCAT+AGG + chr7.4:22289069-22289088 None:intergenic 45.0%
TTCTGCATAGGTGAAGGGTA+TGG + chr7.4:22289057-22289076 None:intergenic 45.0%
!! AGGGTATGGATTTGGTGTTG+TGG + chr7.4:22289043-22289062 None:intergenic 45.0%
!! CTGTGGATTTGGTGGAAAGA+AGG - chr7.4:22289739-22289758 MS.gene064258:CDS 45.0%
!! GTATGGATTTGGTGTTGTGG+AGG + chr7.4:22289040-22289059 None:intergenic 45.0%
!! TGGATTTGGTGGAAAGAAGG+AGG - chr7.4:22289742-22289761 MS.gene064258:CDS 45.0%
ATGTAGGAGAACTCATGGGG+AGG - chr7.4:22289657-22289676 MS.gene064258:CDS 50.0%
GGGAACAACTTTGGACTGTC+TGG - chr7.4:22290339-22290358 MS.gene064258:CDS 50.0%
GGGTTTCCTTGGGAGAAGTT+AGG - chr7.4:22289320-22289339 MS.gene064258:CDS 50.0%
GTTGTGGAGGTAATCGGTGA+GGG + chr7.4:22289027-22289046 None:intergenic 50.0%
TGGTGGAAAGAAGGAGGAAG+TGG - chr7.4:22289748-22289767 MS.gene064258:CDS 50.0%
TGTTGTGGAGGTAATCGGTG+AGG + chr7.4:22289028-22289047 None:intergenic 50.0%
CGGCACAAGCAGTGAGTTAC+AGG - chr7.4:22290183-22290202 MS.gene064258:intron 55.0%
! TGGGCATTATCGGCTGCTAG+CGG - chr7.4:22289991-22290010 MS.gene064258:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 22289000 22290413 22289000 ID=MS.gene064258
chr7.4 mRNA 22289000 22290413 22289000 ID=MS.gene064258.t1;Parent=MS.gene064258
chr7.4 exon 22290289 22290413 22290289 ID=MS.gene064258.t1.exon1;Parent=MS.gene064258.t1
chr7.4 CDS 22290289 22290413 22290289 ID=cds.MS.gene064258.t1;Parent=MS.gene064258.t1
chr7.4 exon 22289152 22290199 22289152 ID=MS.gene064258.t1.exon2;Parent=MS.gene064258.t1
chr7.4 CDS 22289152 22290199 22289152 ID=cds.MS.gene064258.t1;Parent=MS.gene064258.t1
chr7.4 exon 22289000 22289079 22289000 ID=MS.gene064258.t1.exon3;Parent=MS.gene064258.t1
chr7.4 CDS 22289000 22289079 22289000 ID=cds.MS.gene064258.t1;Parent=MS.gene064258.t1
Gene Sequence

>MS.gene064258

ATGTCATTCACGAAGCTCAAAAAGCCCTCACCGATTACCTCCACAACACCAAATCCATACCCTTCACCTATGCAGAACACATTGGCAACAACACTCTCTGTTCCCTCACAAATCTCATTTCAAAGGTACATTTTTCACTACCCACTTTCTCAAATAACATTAAAAGAGTCATTAGGTTTCACCCCATTAACGAATTTGAAGTCTTTTTTGAAAGCATAGGGATAGATTATACTTTGGTTTCTGATTTGTTACCGAATGATAAGCTGTTCTTTTCAGAGGATAGGACTCTTTTAGAAGCTGCTTGTGTGCTTTGTGATTTTGGGTTTCCTTGGGAGAAGTTAGGTGTGTTGTATATGGAGAAGAGTTCTATATTTGGGAAAAGTGTTGAAGAGTTGAAATTGAGGCTTTGTTGGTTTAAAAGATTTGGTTTTGGTAATGTTGAAGTTATTGGGATTTGTTTGGCTTTTCCTTTTGTTTTGAGTGAGGAAGTGGGTCAAATTCAAAAGGGTGTTTTTGGAATTGATGCGTTGTTTAGTGATCTAAAGTTAATTTTCTTGGATAATTCTTTGGGGAGTTATGTTGAGGGAAATGTAGATTCATGGCATGAGGTGTGTAGAAAGTTGCGATTTTTTTACGATTTGAATGGATGGAAGGGTAATGTAGGAGAACTCATGGGGAGGAATAAGAGTATATTTGTTGATCACAAAGAAGAGGAGATAGTACATAAAGTAGAGTATTTCTGTGGATTTGGTGGAAAGAAGGAGGAAGTGGCTTTATTGATTCTGCAATGTCCTGAAGTGTTAAAGCTTGATTTGGAGAAGACGGTGATTAATGTGTTGGAGCTGTTGAAACACTTTGGCATGAGCTCTAAAGATTTGGAGGATGTTATTGAAAATTTTGGTCATGTGTTGGGAACAATTAGAATGGTTAATCTACCAAATGTGATGAGAGCTATGGGTTTGCAAGAATGGTTCTGTGATAAGTTAAAGGGTGGGCATTATCGGCTGCTAGCGGATTATATCACAAGTGATCGCAATGAAGACCGGGATAAAGATTATCAAGATGGTTTGAGGAGAATTCACATTTCACGAGCTCGAGTTCATAGCATTAATAAGTTAAATTTCTTACATAGATTAGGCTTTGGAGAAAATGCATTGACTATGAATCTTTTGGATCGTTTGCACGGCACAAGCAGTGAGTTACAGGAAAGATTTGACTGCCTTCTACGCTCAAGAATTGAATTCTCAAAGCTCTGCATGATGGTAAGAAAAACCCCAAGGATTCTGAACCAAAATTATGAAATTATAGAGCAAAAAGTGAATTTTTTCAACCAGGAAATGGGAACAACTTTGGACTGTCTGGAAACTTTTCCAGCAATGTTGCATTATCACTTGGATGACCGAATTATACCTAG
Protein sequence

>MS.gene064258.t1

MSFTKLKKPSPITSTTPNPYPSPMQNTLATTLSVPSQISFQSIGIDYTLVSDLLPNDKLFFSEDRTLLEAACVLCDFGFPWEKLGVLYMEKSSIFGKSVEELKLRLCWFKRFGFGNVEVIGICLAFPFVLSEEVGQIQKGVFGIDALFSDLKLIFLDNSLGSYVEGNVDSWHEVCRKLRFFYDLNGWKGNVGELMGRNKSIFVDHKEEEIVHKVEYFCGFGGKKEEVALLILQCPEVLKLDLEKTVINVLELLKHFGMSSKDLEDVIENFGHVLGTIRMVNLPNVMRAMGLQEWFCDKLKGGHYRLLADYITSDRNEDRDKDYQDGLRRIHISRARVHSINKLNFLHRLGFGENALTMNLLDRLHGTSSELQERFDCLLRSRIEFSKLCMMEMGTTLDCLETFPAMLHYHLDDRIIP