AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064725


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064725.t1 MTR_3g113070 88.186 237 14 4 1 236 1 224 4.43e-151 419
MS.gene064725.t1 MTR_1g023260 46.595 279 84 10 1 232 1 261 1.84e-66 207
MS.gene064725.t1 MTR_1g023260 55.801 181 45 4 1 149 1 178 7.61e-62 192
MS.gene064725.t1 MTR_3g117320 48.529 204 55 5 1 158 1 200 9.48e-57 180
MS.gene064725.t1 MTR_4g071200 42.647 204 79 5 1 169 1 201 1.46e-49 162
MS.gene064725.t1 MTR_2g099010 34.884 215 114 4 1 203 1 201 2.34e-36 129
MS.gene064725.t1 MTR_2g073370 54.206 107 46 1 1 107 1 104 3.71e-36 129
MS.gene064725.t1 MTR_2g099010 35.814 215 111 4 1 203 1 200 8.22e-36 127
MS.gene064725.t1 MTR_4g067320 52.336 107 48 1 1 107 1 104 1.11e-32 119
MS.gene064725.t1 MTR_4g067320 52.336 107 48 1 1 107 1 104 1.20e-32 119
MS.gene064725.t1 MTR_1g023260 36.620 213 72 7 67 232 57 253 1.27e-27 106
MS.gene064725.t1 MTR_5g021580 33.511 188 104 6 1 186 1 169 6.79e-24 94.7
MS.gene064725.t1 MTR_5g021580 33.511 188 104 6 1 186 1 169 9.45e-24 94.4
MS.gene064725.t1 MTR_1g013450 33.113 151 83 2 5 149 6 144 1.08e-17 80.9
MS.gene064725.t1 MTR_7g018170 34.821 112 61 1 5 116 26 125 3.91e-16 77.0
MS.gene064725.t1 MTR_3g105710 38.947 95 46 1 5 99 6 88 1.24e-14 72.0
MS.gene064725.t1 MTR_4g128930 36.842 95 48 1 5 99 22 104 5.37e-14 70.9
MS.gene064725.t1 MTR_5g069480 31.313 99 56 1 1 99 1 87 1.79e-12 65.9
MS.gene064725.t1 MTR_5g069480 31.313 99 56 1 1 99 1 87 3.26e-12 65.5
MS.gene064725.t1 MTR_2g099010 25.767 163 95 4 56 203 24 175 2.07e-11 62.0
MS.gene064725.t1 MTR_3g082630 33.036 112 59 3 1 112 1 96 2.52e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064725.t1 AT1G75540 38.272 324 107 10 1 235 1 320 6.47e-59 189
MS.gene064725.t1 AT4G39070 60.163 123 46 2 1 122 1 121 3.17e-45 151
MS.gene064725.t1 AT1G78600 53.271 107 47 1 1 107 1 104 4.39e-36 129
MS.gene064725.t1 AT1G78600 53.271 107 47 1 1 107 1 104 7.27e-36 129
MS.gene064725.t1 AT1G06040 54.206 107 46 1 1 107 1 104 8.70e-36 125
MS.gene064725.t1 AT2G31380 54.206 107 46 1 1 107 1 104 3.09e-35 125
MS.gene064725.t1 AT1G06040 54.206 107 46 1 1 107 1 104 5.82e-35 125
MS.gene064725.t1 AT4G10240 48.182 110 50 2 1 107 1 106 1.64e-30 111
MS.gene064725.t1 AT2G21320 41.322 121 65 2 1 121 1 115 5.63e-24 94.7
MS.gene064725.t1 AT4G38960 42.982 114 59 2 1 114 1 108 6.50e-24 94.7
MS.gene064725.t1 AT4G38960 42.982 114 59 2 1 114 1 108 6.50e-24 94.7
MS.gene064725.t1 AT2G24790 33.835 133 66 3 5 129 8 126 9.87e-17 76.6
MS.gene064725.t1 AT2G24790 40.000 95 45 1 5 99 8 90 2.93e-16 76.6
MS.gene064725.t1 AT4G38960 31.210 157 59 3 1 114 1 151 4.79e-16 74.7
MS.gene064725.t1 AT5G15850 35.398 113 61 1 5 117 12 112 7.10e-16 75.9
MS.gene064725.t1 AT5G24930 37.895 95 47 1 5 99 6 88 5.72e-15 73.6
MS.gene064725.t1 AT3G02380 35.088 114 59 2 5 115 16 117 1.58e-14 72.0
MS.gene064725.t1 AT2G33500 35.922 103 54 1 3 105 10 100 2.11e-13 69.3
MS.gene064725.t1 AT2G33500 35.922 103 54 1 3 105 10 100 2.25e-13 68.9
MS.gene064725.t1 AT1G28050 37.500 104 53 1 2 105 6 97 2.35e-13 68.9
MS.gene064725.t1 AT5G15840 30.435 115 68 1 5 119 20 122 4.98e-13 67.4
MS.gene064725.t1 AT5G15840 32.632 95 52 1 5 99 20 102 7.63e-13 67.4
MS.gene064725.t1 AT2G47890 40.476 84 38 2 5 88 13 84 2.61e-11 62.0
MS.gene064725.t1 AT2G47890 40.476 84 38 2 5 88 13 84 2.68e-11 62.4
MS.gene064725.t1 AT2G47890 40.476 84 38 2 5 88 13 84 4.04e-11 62.4

Find 60 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGGTTGGGAGAATTATT+AGG 0.172908 3.4:-97561307 None:intergenic
TAAGATGTGACAATGTTAAT+TGG 0.192450 3.4:-97562027 None:intergenic
AAGAAACCTATTATGCTTCT+TGG 0.240931 3.4:-97561604 None:intergenic
ACCAATTTCAGCAGCAGTTT+GGG 0.246710 3.4:+97561972 MS.gene064725:CDS
CCCCAAACTGCTGCTGAAAT+TGG 0.248979 3.4:-97561973 None:intergenic
TATTCATGAGGAAAATATTC+TGG 0.282344 3.4:+97561848 MS.gene064725:CDS
CCAACTCTTCATGAAGAAAA+TGG 0.301498 3.4:+97561663 MS.gene064725:CDS
CACCAATTTCAGCAGCAGTT+TGG 0.303898 3.4:+97561971 MS.gene064725:CDS
GAGAGAGAAGCGACGGTGTT+TGG 0.360255 3.4:-97561278 None:intergenic
AACACACCAAGAAGCATAAT+AGG 0.390006 3.4:+97561598 MS.gene064725:CDS
AAGATGTGACAATGTTAATT+GGG 0.396257 3.4:-97562026 None:intergenic
CATAATAGGTTTCTTCTAAG+TGG 0.399657 3.4:+97561612 MS.gene064725:CDS
GAGTGTGATTTGAAGATTCA+TGG 0.427331 3.4:+97561567 MS.gene064725:CDS
TGAAGAGTTGGAGGAGGAGC+AGG 0.431012 3.4:-97561651 None:intergenic
CTACAACAGCAGAATCATAT+TGG 0.434122 3.4:+97561780 MS.gene064725:CDS
AATGGTTGGGAGAATTATTA+GGG 0.446787 3.4:-97561306 None:intergenic
TCATGAGAGGGAACAGAAGA+AGG 0.454520 3.4:-97561756 None:intergenic
GTGGTCGCAGGTGGAGCATA+AGG 0.456556 3.4:-97561227 None:intergenic
GAACAGAAGAAGGGGATTCA+AGG 0.477624 3.4:-97561746 None:intergenic
ATAATAGGTTTCTTCTAAGT+GGG 0.482873 3.4:+97561613 MS.gene064725:CDS
TTCTCTAGCGAGAGCATGTG+TGG 0.496184 3.4:+97561876 MS.gene064725:CDS
TCACAGAGAGGGAAATGGTT+GGG 0.504950 3.4:-97561319 None:intergenic
AAATATCACAGAGAGGGAAA+TGG 0.517933 3.4:-97561324 None:intergenic
AGAGAAGCGACGGTGTTTGG+AGG 0.521730 3.4:-97561275 None:intergenic
ATGGTGGACACGGTGGTCGC+AGG 0.523492 3.4:-97561239 None:intergenic
CAAACTGCTGCTGAAATTGG+TGG 0.525028 3.4:-97561970 None:intergenic
GTGGACACGGTGGTCGCAGG+TGG 0.531543 3.4:-97561236 None:intergenic
AAATCTTCAAACTTCCAACC+TGG 0.533797 3.4:-97561723 None:intergenic
GGTTGGGAGAATTATTAGGG+TGG 0.538692 3.4:-97561303 None:intergenic
TGGAGGCGAGTTTGTTAGCA+TGG 0.539793 3.4:-97561258 None:intergenic
TGTGACAATGTTAATTGGGA+GGG 0.542911 3.4:-97562022 None:intergenic
CATGAGAGGGAACAGAAGAA+GGG 0.544618 3.4:-97561755 None:intergenic
TGCTGTTGTAGTTCATGAGA+GGG 0.552947 3.4:-97561768 None:intergenic
GTTTGTTAGCATGGTGGACA+CGG 0.562165 3.4:-97561249 None:intergenic
TCTTCATGAAGAGTTGGAGG+AGG 0.568381 3.4:-97561657 None:intergenic
CTTTGTTTGTAGGAAAGAAG+AGG 0.568458 3.4:+97561504 MS.gene064725:intron
CTGCTGTTGTAGTTCATGAG+AGG 0.569968 3.4:-97561769 None:intergenic
GCATAAGGCGGCTTCATCCG+CGG 0.570214 3.4:-97561212 None:intergenic
AGTGCGACATCTGTAACAAA+CGG 0.576581 3.4:+97561170 MS.gene064725:CDS
TACTTGATAAACACTATCCC+AGG 0.579028 3.4:+97561705 MS.gene064725:CDS
CCAATTTCAGCAGCAGTTTG+GGG 0.580053 3.4:+97561973 MS.gene064725:CDS
AATCTTCAAACTTCCAACCT+GGG 0.582408 3.4:-97561722 None:intergenic
GAATAATACAGTGGTGCTTG+AGG 0.582637 3.4:-97561909 None:intergenic
TTGGGAGGGATTGTTATCTG+TGG 0.583370 3.4:-97562008 None:intergenic
CGACATCTGTAACAAACGGC+AGG 0.587499 3.4:+97561174 MS.gene064725:CDS
ATCACAGAGAGGGAAATGGT+TGG 0.589033 3.4:-97561320 None:intergenic
GTTTGTTACAGATGTCGCAC+TGG 0.600679 3.4:-97561168 None:intergenic
TCTCTAGCGAGAGCATGTGT+GGG 0.602430 3.4:+97561877 MS.gene064725:CDS
ATGTGACAATGTTAATTGGG+AGG 0.602797 3.4:-97562023 None:intergenic
GTCGCAGGTGGAGCATAAGG+CGG 0.608572 3.4:-97561224 None:intergenic
CGAGGCAAATATCACAGAGA+GGG 0.613147 3.4:-97561330 None:intergenic
ATGAGAGGGAACAGAAGAAG+GGG 0.616850 3.4:-97561754 None:intergenic
CAATTTCAGCAGCAGTTTGG+GGG 0.628510 3.4:+97561974 MS.gene064725:CDS
TGATAAACACTATCCCAGGT+TGG 0.630031 3.4:+97561709 MS.gene064725:CDS
GGTGGTTGAGAGAGAAGCGA+CGG 0.648653 3.4:-97561285 None:intergenic
TGTTAATTATCATATTCATG+AGG 0.653612 3.4:+97561836 MS.gene064725:CDS
AGGCGAGTTTGTTAGCATGG+TGG 0.655308 3.4:-97561255 None:intergenic
TGTTAGCATGGTGGACACGG+TGG 0.691209 3.4:-97561246 None:intergenic
ACGAGGCAAATATCACAGAG+AGG 0.701262 3.4:-97561331 None:intergenic
GGCTTCTTTATTCTGCACCG+CGG 0.708284 3.4:+97561195 MS.gene064725:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACATAAAAGAATGAATAT+TGG - chr3.4:97561365-97561384 None:intergenic 15.0%
!! TGTTAATTATCATATTCATG+AGG + chr3.4:97561836-97561855 MS.gene064725:CDS 20.0%
!!! ACTGTATTATTCTGAAAAAA+TGG + chr3.4:97561920-97561939 MS.gene064725:CDS 20.0%
!!! ATTTTTTCAGAATAATACAG+TGG - chr3.4:97561921-97561940 None:intergenic 20.0%
! ATAATAGGTTTCTTCTAAGT+GGG + chr3.4:97561613-97561632 MS.gene064725:CDS 25.0%
! TATTCATGAGGAAAATATTC+TGG + chr3.4:97561848-97561867 MS.gene064725:CDS 25.0%
AAATGGTTGGGAGAATTATT+AGG - chr3.4:97561310-97561329 None:intergenic 30.0%
AAGAAACCTATTATGCTTCT+TGG - chr3.4:97561607-97561626 None:intergenic 30.0%
AATGGTTGGGAGAATTATTA+GGG - chr3.4:97561309-97561328 None:intergenic 30.0%
CATAATAGGTTTCTTCTAAG+TGG + chr3.4:97561612-97561631 MS.gene064725:CDS 30.0%
! GAGTTTGTTTCTTTGTTTGT+AGG + chr3.4:97561494-97561513 MS.gene064725:intron 30.0%
!!! AAGAGGTTTTGTTTTTTGTC+AGG + chr3.4:97561521-97561540 MS.gene064725:CDS 30.0%
AAATATCACAGAGAGGGAAA+TGG - chr3.4:97561327-97561346 None:intergenic 35.0%
AAATCTTCAAACTTCCAACC+TGG - chr3.4:97561726-97561745 None:intergenic 35.0%
AATCTTCAAACTTCCAACCT+GGG - chr3.4:97561725-97561744 None:intergenic 35.0%
CCAACTCTTCATGAAGAAAA+TGG + chr3.4:97561663-97561682 MS.gene064725:CDS 35.0%
CTACAACAGCAGAATCATAT+TGG + chr3.4:97561780-97561799 MS.gene064725:CDS 35.0%
GAATATTGGAGAGAATTACG+AGG - chr3.4:97561351-97561370 None:intergenic 35.0%
GAGTGTGATTTGAAGATTCA+TGG + chr3.4:97561567-97561586 MS.gene064725:CDS 35.0%
TACTTGATAAACACTATCCC+AGG + chr3.4:97561705-97561724 MS.gene064725:CDS 35.0%
! AACACACCAAGAAGCATAAT+AGG + chr3.4:97561598-97561617 MS.gene064725:CDS 35.0%
! CCATTTTCTTCATGAAGAGT+TGG - chr3.4:97561666-97561685 None:intergenic 35.0%
! CTTTGTTTGTAGGAAAGAAG+AGG + chr3.4:97561504-97561523 MS.gene064725:intron 35.0%
! TTTTCTTCATGAAGAGTTGG+AGG - chr3.4:97561663-97561682 None:intergenic 35.0%
ACCAATTTCAGCAGCAGTTT+GGG + chr3.4:97561972-97561991 MS.gene064725:CDS 40.0%
AGTGCGACATCTGTAACAAA+CGG + chr3.4:97561170-97561189 MS.gene064725:CDS 40.0%
GAATAATACAGTGGTGCTTG+AGG - chr3.4:97561912-97561931 None:intergenic 40.0%
TGATAAACACTATCCCAGGT+TGG + chr3.4:97561709-97561728 MS.gene064725:CDS 40.0%
TGCTGTTGTAGTTCATGAGA+GGG - chr3.4:97561771-97561790 None:intergenic 40.0%
ACGAGGCAAATATCACAGAG+AGG - chr3.4:97561334-97561353 None:intergenic 45.0%
ATCACAGAGAGGGAAATGGT+TGG - chr3.4:97561323-97561342 None:intergenic 45.0%
ATGAGAGGGAACAGAAGAAG+GGG - chr3.4:97561757-97561776 None:intergenic 45.0%
CAAACTGCTGCTGAAATTGG+TGG - chr3.4:97561973-97561992 None:intergenic 45.0%
CAATTTCAGCAGCAGTTTGG+GGG + chr3.4:97561974-97561993 MS.gene064725:CDS 45.0%
CACCAATTTCAGCAGCAGTT+TGG + chr3.4:97561971-97561990 MS.gene064725:CDS 45.0%
CATGAGAGGGAACAGAAGAA+GGG - chr3.4:97561758-97561777 None:intergenic 45.0%
CCAATTTCAGCAGCAGTTTG+GGG + chr3.4:97561973-97561992 MS.gene064725:CDS 45.0%
CGAGGCAAATATCACAGAGA+GGG - chr3.4:97561333-97561352 None:intergenic 45.0%
CTGCTGTTGTAGTTCATGAG+AGG - chr3.4:97561772-97561791 None:intergenic 45.0%
GAACAGAAGAAGGGGATTCA+AGG - chr3.4:97561749-97561768 None:intergenic 45.0%
GGTTGGGAGAATTATTAGGG+TGG - chr3.4:97561306-97561325 None:intergenic 45.0%
TCACAGAGAGGGAAATGGTT+GGG - chr3.4:97561322-97561341 None:intergenic 45.0%
TCATGAGAGGGAACAGAAGA+AGG - chr3.4:97561759-97561778 None:intergenic 45.0%
TCTTCATGAAGAGTTGGAGG+AGG - chr3.4:97561660-97561679 None:intergenic 45.0%
TTGGGAGGGATTGTTATCTG+TGG - chr3.4:97562011-97562030 None:intergenic 45.0%
! GTTTGTTACAGATGTCGCAC+TGG - chr3.4:97561171-97561190 None:intergenic 45.0%
!! GTTTGTTAGCATGGTGGACA+CGG - chr3.4:97561252-97561271 None:intergenic 45.0%
CCCCAAACTGCTGCTGAAAT+TGG - chr3.4:97561976-97561995 None:intergenic 50.0%
CGACATCTGTAACAAACGGC+AGG + chr3.4:97561174-97561193 MS.gene064725:CDS 50.0%
GGCTTCTTTATTCTGCACCG+CGG + chr3.4:97561195-97561214 MS.gene064725:CDS 50.0%
! TCTCTAGCGAGAGCATGTGT+GGG + chr3.4:97561877-97561896 MS.gene064725:CDS 50.0%
! TTCTCTAGCGAGAGCATGTG+TGG + chr3.4:97561876-97561895 MS.gene064725:CDS 50.0%
!! AGGCGAGTTTGTTAGCATGG+TGG - chr3.4:97561258-97561277 None:intergenic 50.0%
!! TGGAGGCGAGTTTGTTAGCA+TGG - chr3.4:97561261-97561280 None:intergenic 50.0%
AGAGAAGCGACGGTGTTTGG+AGG - chr3.4:97561278-97561297 None:intergenic 55.0%
GAGAGAGAAGCGACGGTGTT+TGG - chr3.4:97561281-97561300 None:intergenic 55.0%
GGTGGTTGAGAGAGAAGCGA+CGG - chr3.4:97561288-97561307 None:intergenic 55.0%
TGAAGAGTTGGAGGAGGAGC+AGG - chr3.4:97561654-97561673 None:intergenic 55.0%
!! TGTTAGCATGGTGGACACGG+TGG - chr3.4:97561249-97561268 None:intergenic 55.0%
GCATAAGGCGGCTTCATCCG+CGG - chr3.4:97561215-97561234 None:intergenic 60.0%
GTCGCAGGTGGAGCATAAGG+CGG - chr3.4:97561227-97561246 None:intergenic 60.0%
GTGGTCGCAGGTGGAGCATA+AGG - chr3.4:97561230-97561249 None:intergenic 60.0%
!! ATGGTGGACACGGTGGTCGC+AGG - chr3.4:97561242-97561261 None:intergenic 65.0%
GTGGACACGGTGGTCGCAGG+TGG - chr3.4:97561239-97561258 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 97561160 97562034 97561160 ID=MS.gene064725
chr3.4 mRNA 97561160 97562034 97561160 ID=MS.gene064725.t1;Parent=MS.gene064725
chr3.4 exon 97561160 97561351 97561160 ID=MS.gene064725.t1.exon1;Parent=MS.gene064725.t1
chr3.4 CDS 97561160 97561351 97561160 ID=cds.MS.gene064725.t1;Parent=MS.gene064725.t1
chr3.4 exon 97561516 97562034 97561516 ID=MS.gene064725.t1.exon2;Parent=MS.gene064725.t1
chr3.4 CDS 97561516 97562034 97561516 ID=cds.MS.gene064725.t1;Parent=MS.gene064725.t1
Gene Sequence

>MS.gene064725

ATGAAGATCCAGTGCGACATCTGTAACAAACGGCAGGCTTCTTTATTCTGCACCGCGGATGAAGCCGCCTTATGCTCCACCTGCGACCACCGTGTCCACCATGCTAACAAACTCGCCTCCAAACACCGTCGCTTCTCTCTCAACCACCCTAATAATTCTCCCAACCATTTCCCTCTCTGTGATATTTGCCTCGTAATTCTCTCCAATATTCATTCTTTTATGTATACTATACTTGTTATATATAGAAAGAGACTAGCTAGTAATTATATACTATTAATTTCATTTGATTATATCAACTTATGATTCATGAATTAACATATAATATTGATACATTGAGTTTGTTTCTTTGTTTGTAGGAAAGAAGAGGTTTTGTTTTTTGTCAGGAAGATAGAGCAATTGTGTGCAAAGAGTGTGATTTGAAGATTCATGGTGTCAACGAACACACCAAGAAGCATAATAGGTTTCTTCTAAGTGGGATCAAGTTGTACTCTCCTGCTCCTCCTCCAACTCTTCATGAAGAAAATGGTAGTTTTACTATATCTGAGTACTTGATAAACACTATCCCAGGTTGGAAGTTTGAAGATTTCCTTGAATCCCCTTCTTCTGTTCCCTCTCATGAACTACAACAGCAGAATCATATTGGTAAAGATACTACATTGTCAACAGTGCATGATGATGTTAATTATCATATTCATGAGGAAAATATTCTGGTTTCTTTCTCTAGCGAGAGCATGTGTGGGATCTGTGTTCCTCAAGCACCACTGTATTATTCTGAAAAAATGGATAACAAATCAGACACAATTAATGAAGCCACCAATTTCAGCAGCAGTTTGGGGGATTTCACAGTTCCACAGATAACAATCCCTCCCAATTAA

Protein sequence

>MS.gene064725.t1

MKIQCDICNKRQASLFCTADEAALCSTCDHRVHHANKLASKHRRFSLNHPNNSPNHFPLCDICLERRGFVFCQEDRAIVCKECDLKIHGVNEHTKKHNRFLLSGIKLYSPAPPPTLHEENGSFTISEYLINTIPGWKFEDFLESPSSVPSHELQQQNHIGKDTTLSTVHDDVNYHIHEENILVSFSSESMCGICVPQAPLYYSEKMDNKSDTINEATNFSSSLGDFTVPQITIPPN