AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064843

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064843.t1 MTR_0645s0010 56.589 129 52 2 252 380 1 125 7.69e-40 143
MS.gene064843.t1 MTR_7g446520 38.222 225 111 4 6 218 47 255 9.19e-38 144
MS.gene064843.t1 MTR_8g035530 77.632 76 16 1 28 102 1 76 7.17e-32 119
MS.gene064843.t1 MTR_7g028855 42.763 152 82 1 33 179 112 263 5.74e-30 124
MS.gene064843.t1 MTR_7g446480 37.500 152 89 1 113 264 140 285 1.24e-26 111
MS.gene064843.t1 MTR_5g076070 30.723 166 110 2 38 198 50 215 2.31e-24 104
MS.gene064843.t1 MTR_7g446510 45.872 109 56 1 113 218 155 263 2.45e-24 105
MS.gene064843.t1 MTR_3g080590 54.651 86 39 0 5 90 69 154 8.21e-24 99.0
MS.gene064843.t1 MTR_4g035820 28.242 347 204 10 44 348 78 421 1.73e-23 106
MS.gene064843.t1 MTR_1g070355 30.636 173 112 2 41 208 74 243 1.63e-21 99.8
MS.gene064843.t1 MTR_0402s0040 28.829 222 131 5 45 262 30 228 2.56e-21 98.6
MS.gene064843.t1 MTR_6g022080 29.825 171 113 2 34 198 78 247 4.24e-21 94.0
MS.gene064843.t1 MTR_6g022070 31.298 131 90 0 208 338 35 165 7.89e-19 89.0
MS.gene064843.t1 MTR_6g051425 27.419 186 124 4 40 215 16 200 3.13e-15 78.2
MS.gene064843.t1 MTR_7g061980 34.862 109 65 2 44 148 13 119 3.51e-14 70.1
MS.gene064843.t1 MTR_1g008290 30.833 120 72 3 81 200 1 109 8.36e-13 67.0
MS.gene064843.t1 MTR_5g015480 33.077 130 80 3 45 167 60 189 1.30e-12 69.3
MS.gene064843.t1 MTR_8g468460 35.714 98 62 1 296 393 9 105 2.32e-12 64.3
MS.gene064843.t1 MTR_4g067290 35.000 100 63 2 44 141 59 158 2.91e-12 67.8
MS.gene064843.t1 MTR_4g067290 35.000 100 63 2 44 141 59 158 3.30e-12 67.4
MS.gene064843.t1 MTR_4g067290 35.000 100 63 2 44 141 77 176 5.24e-12 67.4
MS.gene064843.t1 MTR_4g067290 35.000 100 63 2 44 141 79 178 5.34e-12 67.4
MS.gene064843.t1 MTR_4g067290 35.000 100 63 2 44 141 79 178 5.54e-12 67.4
MS.gene064843.t1 MTR_4g067290 35.000 100 63 2 44 141 119 218 6.05e-12 67.8
MS.gene064843.t1 MTR_2g098920 35.514 107 67 2 39 143 78 184 6.37e-12 67.0
MS.gene064843.t1 MTR_7g074790 29.814 161 89 3 44 200 13 153 9.42e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064843.t1 AT4G38180 28.821 229 162 1 210 438 411 638 4.01e-30 127
MS.gene064843.t1 AT4G38180 34.562 217 132 6 2 209 32 247 2.62e-22 103
MS.gene064843.t1 AT2G27110 29.302 215 149 2 220 434 368 579 6.86e-27 117
MS.gene064843.t1 AT2G27110 29.302 215 149 2 220 434 368 579 6.86e-27 117
MS.gene064843.t1 AT2G27110 29.302 215 149 2 220 434 223 434 7.75e-27 117
MS.gene064843.t1 AT4G38170 25.333 225 167 1 210 434 176 399 3.90e-26 114
MS.gene064843.t1 AT4G15090 24.476 286 206 3 154 439 339 614 6.23e-26 114
MS.gene064843.t1 AT4G15090 24.476 286 206 3 154 439 339 614 6.23e-26 114
MS.gene064843.t1 AT4G15090 24.476 286 206 3 154 439 339 614 6.23e-26 114
MS.gene064843.t1 AT4G15090 24.476 286 206 3 154 439 339 614 6.23e-26 114
MS.gene064843.t1 AT4G15090 24.476 286 206 3 154 439 339 614 6.23e-26 114
MS.gene064843.t1 AT1G76320 27.602 221 156 3 215 435 329 545 3.60e-25 112
MS.gene064843.t1 AT1G76320 27.602 221 156 3 215 435 329 545 3.95e-25 112
MS.gene064843.t1 AT1G76320 27.602 221 156 3 215 435 329 545 3.95e-25 112
MS.gene064843.t1 AT1G76320 27.602 221 156 3 215 435 329 545 7.39e-25 110
MS.gene064843.t1 AT5G18960 25.547 274 197 4 210 482 508 775 2.56e-24 109
MS.gene064843.t1 AT5G18960 36.434 129 58 5 13 139 25 131 4.49e-11 67.0
MS.gene064843.t1 AT5G18960 25.547 274 197 4 210 482 508 775 2.56e-24 109
MS.gene064843.t1 AT5G18960 36.434 129 58 5 13 139 25 131 4.49e-11 67.0
MS.gene064843.t1 AT3G22170 26.201 229 167 2 211 439 397 623 2.81e-23 106
MS.gene064843.t1 AT3G22170 26.201 229 167 2 211 439 397 623 2.81e-23 106
MS.gene064843.t1 AT3G06250 24.910 277 195 6 210 482 484 751 1.96e-22 103
MS.gene064843.t1 AT3G06250 24.910 277 195 6 210 482 484 751 1.96e-22 103
MS.gene064843.t1 AT3G06250 24.910 277 195 6 210 482 484 751 1.96e-22 103
MS.gene064843.t1 AT4G19990 24.696 247 180 4 205 449 330 572 3.75e-22 102
MS.gene064843.t1 AT4G19990 24.696 247 180 4 205 449 330 572 3.75e-22 102
MS.gene064843.t1 AT4G19990 24.696 247 180 4 205 449 273 515 4.58e-22 102
MS.gene064843.t1 AT4G19990 24.696 247 180 4 205 449 330 572 5.12e-22 102
MS.gene064843.t1 AT4G19990 24.696 247 180 4 205 449 330 572 5.12e-22 102
MS.gene064843.t1 AT4G19990 24.696 247 180 4 205 449 330 572 6.30e-22 102
MS.gene064843.t1 AT4G19990 24.696 247 180 4 205 449 344 586 6.92e-22 101
MS.gene064843.t1 AT2G32250 23.345 287 203 5 195 472 275 553 5.46e-19 92.4
MS.gene064843.t1 AT2G32250 23.345 287 203 5 195 472 316 594 5.64e-19 92.4
MS.gene064843.t1 AT2G32250 23.345 287 203 5 195 472 316 594 5.64e-19 92.4
MS.gene064843.t1 AT2G32250 23.345 287 203 5 195 472 316 594 5.85e-19 92.4
MS.gene064843.t1 AT2G32250 23.345 287 203 5 195 472 316 594 5.85e-19 92.4
MS.gene064843.t1 AT2G32250 23.345 287 203 5 195 472 316 594 5.85e-19 92.4
MS.gene064843.t1 AT2G32250 23.345 287 203 5 195 472 275 553 6.23e-19 92.4
MS.gene064843.t1 AT2G32250 23.345 287 203 5 195 472 316 594 9.30e-19 91.7
MS.gene064843.t1 AT1G52520 24.242 231 168 2 211 434 408 638 2.27e-18 90.5
MS.gene064843.t1 AT1G80010 24.167 240 175 5 214 447 436 674 3.22e-17 86.7
MS.gene064843.t1 AT3G07500 36.842 152 82 6 16 157 3 150 1.01e-15 77.4
MS.gene064843.t1 AT1G10240 21.339 239 170 6 201 431 376 604 2.22e-13 74.3
MS.gene064843.t1 AT1G10240 29.545 176 115 5 44 210 51 226 4.21e-12 70.1
MS.gene064843.t1 AT1G10240 21.339 239 170 6 201 431 376 604 2.22e-13 74.3
MS.gene064843.t1 AT1G10240 29.545 176 115 5 44 210 51 226 4.21e-12 70.1
MS.gene064843.t1 AT4G12850 37.705 122 71 3 39 156 9 129 1.67e-12 67.4
MS.gene064843.t1 AT4G12850 40.196 102 59 2 39 139 9 109 2.41e-12 65.5
MS.gene064843.t1 AT3G59470 37.963 108 63 3 39 143 68 174 3.32e-12 67.8
MS.gene064843.t1 AT3G59470 37.963 108 63 3 39 143 68 174 3.32e-12 67.8
MS.gene064843.t1 AT3G59470 37.963 108 63 3 39 143 80 186 4.50e-12 67.8
MS.gene064843.t1 AT4G12850 40.196 102 59 2 39 139 9 109 5.55e-12 64.7

Find 144 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTAATAAATGGAGAGTTTC+TGG 0.125465 3.3:-94264790 MS.gene064843:CDS
GTCTCATGGCACAAATAATA+TGG 0.181170 3.3:+94263462 None:intergenic
GTAATCATCCTTTGCTGATT+TGG 0.196309 3.3:+94263391 None:intergenic
CATCTTTCCATGTTACATTT+TGG 0.217563 3.3:+94262486 None:intergenic
GCAGACACTGTCCTGCAATT+TGG 0.218987 3.3:+94261444 None:intergenic
AGTTGTCTGCTCGTGGAAAT+TGG 0.230194 3.3:+94262324 None:intergenic
AAAGTAGATGCAATTTGATT+TGG 0.270824 3.3:+94262386 None:intergenic
CAGACACTGTCCTGCAATTT+GGG 0.276633 3.3:+94261445 None:intergenic
AAAATTGATGCACCTTGATT+TGG 0.276898 3.3:+94262350 None:intergenic
TCACTCAATTGACGGTTCTT+AGG 0.285255 3.3:+94264712 None:intergenic
AAGACCAATCCATCTGTTAT+TGG 0.288862 3.3:-94263176 MS.gene064843:CDS
TAAATGTTGATACTAATAAA+TGG 0.290315 3.3:-94264801 MS.gene064843:CDS
AACTCGTTATCATGTCCTTT+TGG 0.298312 3.3:-94261527 MS.gene064843:CDS
CATTCAAGTTCTGTTGGATA+TGG 0.304475 3.3:-94262293 MS.gene064843:CDS
CCTTTGGCCTTGGCTTTGAT+AGG 0.324333 3.3:+94263149 None:intergenic
TTGGCGCCGGTAAAATAATT+AGG 0.326324 3.3:+94262449 None:intergenic
ATACAGGCACAAAGAGTTAA+TGG 0.341245 3.3:-94263774 MS.gene064843:CDS
GTTGTCTGCTCGTGGAAATT+GGG 0.345810 3.3:+94262325 None:intergenic
ACGAGGGGCACCTTTGGCCT+TGG 0.345904 3.3:+94263139 None:intergenic
TGAAGAAGGAAAAGAGGATA+TGG 0.353695 3.3:-94265094 MS.gene064843:CDS
TGGATACTTACAAGAAGATT+AGG 0.354642 3.3:-94265074 MS.gene064843:CDS
AACATCACGTCCAGCTAGTT+CGG 0.359681 3.3:+94263040 None:intergenic
GAAAGGAGGTCATGAGAGTC+TGG 0.365097 3.3:-94264611 MS.gene064843:CDS
AGACATAAATGGTGAGGATT+TGG 0.368884 3.3:-94265139 MS.gene064843:CDS
ATAGCCTCTTTATAATCATC+TGG 0.369789 3.3:+94263260 None:intergenic
GCAATTTGATTTGGAAATTG+AGG 0.377041 3.3:+94262395 None:intergenic
CTTCTTACGAGGGGCACCTT+TGG 0.378868 3.3:+94263133 None:intergenic
AAGATCGGAACACTTTGAAT+TGG 0.379246 3.3:+94262419 None:intergenic
CCCACAGTTGCCCAAATTGC+AGG 0.386706 3.3:-94261455 MS.gene064843:CDS
GCAAACGATGGAGACATAAA+TGG 0.387588 3.3:-94265150 MS.gene064843:CDS
ACAATTTATCACACAATACA+TGG 0.415400 3.3:-94263976 MS.gene064843:CDS
AAGTTTAAGATGAATAGATT+TGG 0.423772 3.3:-94263581 MS.gene064843:CDS
TCTGATAAAGCCCTTGTTGA+TGG 0.424223 3.3:-94264688 MS.gene064843:CDS
CTGATAGTCTAATTTGCAAA+AGG 0.431391 3.3:-94263418 MS.gene064843:CDS
CGAGTTTATGCCTTCACACC+TGG 0.432567 3.3:+94263871 None:intergenic
GTATCTAGTTTATCTTGATA+AGG 0.436094 3.3:-94263543 MS.gene064843:CDS
TATCAGATTCACTCAATTGA+CGG 0.439464 3.3:+94264704 None:intergenic
CCATCACATTCAAGTTCTGT+TGG 0.440650 3.3:-94262299 MS.gene064843:CDS
TGTAGACAGTTTGAACGGAT+TGG 0.441359 3.3:-94263497 MS.gene064843:CDS
GAAGGAAAGGCAAGAATAGA+AGG 0.447532 3.3:-94264550 MS.gene064843:intron
AGAAGAAAACATCCAGGAAA+AGG 0.448830 3.3:-94263112 MS.gene064843:CDS
ACATCACGTCCAGCTAGTTC+GGG 0.451712 3.3:+94263041 None:intergenic
CAATTTGGGCAACTGTGGGA+TGG 0.454366 3.3:+94261459 None:intergenic
TATTATTTGTGCCATGAGAC+AGG 0.458720 3.3:-94263459 MS.gene064843:CDS
CAATAAAAGAATATCAAAAC+AGG 0.462051 3.3:-94262794 MS.gene064843:intron
GGATACTTACAAGAAGATTA+GGG 0.465987 3.3:-94265073 MS.gene064843:CDS
CAAGAAGATTAGGGAATTGA+CGG 0.467663 3.3:-94265064 MS.gene064843:CDS
AGGAACGAAATGAGCATAAC+GGG 0.473396 3.3:+94264732 None:intergenic
ATTTATGTCTCCATCGTTTG+CGG 0.473976 3.3:+94265152 None:intergenic
AATGGTGTGTTTAATGAAGA+AGG 0.477078 3.3:-94265108 MS.gene064843:CDS
GAAATCTATGCGGATTGTCA+TGG 0.487261 3.3:-94264985 MS.gene064843:CDS
TCTCATGGCACAAATAATAT+GGG 0.492357 3.3:+94263463 None:intergenic
ATAGAGCTGTAAAAGAATAC+AGG 0.492679 3.3:-94263790 MS.gene064843:CDS
GGATGATGATATAAGAGGAT+TGG 0.496802 3.3:-94265043 MS.gene064843:CDS
TTGACGGATGATGATATAAG+AGG 0.497315 3.3:-94265048 MS.gene064843:CDS
CTCAATTTCAAATTTGTCCA+CGG 0.497600 3.3:+94264021 None:intergenic
TATAACTACTTATACAATGA+AGG 0.501991 3.3:-94264568 MS.gene064843:CDS
TTTGGAAATTGAGGAAAGAT+CGG 0.506335 3.3:+94262404 None:intergenic
AGTCATGTGCTCCTGTCTCA+TGG 0.506642 3.3:+94263448 None:intergenic
TCCTGCAATTTGGGCAACTG+TGG 0.507825 3.3:+94261454 None:intergenic
ATCACCAATAACAGATGGAT+TGG 0.508027 3.3:+94263172 None:intergenic
AGGATTGGAATTTGATTCAG+AGG 0.508865 3.3:-94265028 MS.gene064843:CDS
GATGTAAACAAAGATGATGA+TGG 0.508994 3.3:-94264943 MS.gene064843:CDS
TAGATTGAAAATGAAGGGAT+TGG 0.509056 3.3:-94262703 MS.gene064843:CDS
TTGTACTAGATTGAAAATGA+AGG 0.514645 3.3:-94262709 MS.gene064843:intron
GAGGATTTGGCCAACGGTAA+TGG 0.518248 3.3:-94265126 MS.gene064843:CDS
AGTCTGGTTGATTTCATGCA+TGG 0.520881 3.3:-94263818 MS.gene064843:CDS
AATTTGAAATTGAGTGGAAG+AGG 0.523012 3.3:-94264012 MS.gene064843:CDS
AGATGATTATAAAGAGGCTA+TGG 0.524840 3.3:-94263258 MS.gene064843:CDS
AAAAGCCAATCTCATCCAAA+AGG 0.525339 3.3:+94261512 None:intergenic
AATTGGAACACCGTTGGCGC+CGG 0.526797 3.3:+94262436 None:intergenic
TGCGGATTGTCATGGGTTTG+TGG 0.527631 3.3:-94264977 MS.gene064843:CDS
TTGTCTGCTCGTGGAAATTG+GGG 0.529245 3.3:+94262326 None:intergenic
CAAAACTGTCCCGAACTAGC+TGG 0.529670 3.3:-94263050 MS.gene064843:CDS
AGAAACAAAGTACATATTCG+AGG 0.529955 3.3:-94264867 MS.gene064843:CDS
ACTTTGAATTGGAACACCGT+TGG 0.530199 3.3:+94262430 None:intergenic
AAATCTATGCGGATTGTCAT+GGG 0.530849 3.3:-94264984 MS.gene064843:CDS
GGGCAACTGTGGGATGGGTG+AGG 0.532080 3.3:+94261465 None:intergenic
ATAGTCTAATTTGCAAAAGG+TGG 0.533689 3.3:-94263415 MS.gene064843:CDS
CAACAGAACTTGAATGTGAT+GGG 0.534436 3.3:+94262300 None:intergenic
CAAAATGTAACATGGAAAGA+TGG 0.535009 3.3:-94262485 MS.gene064843:CDS
CCAACAGAACTTGAATGTGA+TGG 0.535015 3.3:+94262299 None:intergenic
ATTTAAATGGCCGCAAACGA+TGG 0.539363 3.3:-94265162 None:intergenic
ATGATAACGAGTTGGCCTAC+TGG 0.539528 3.3:+94261537 None:intergenic
TGCAGAACACTTAGTTCGAA+TGG 0.539821 3.3:+94264840 None:intergenic
TGTACTAGATTGAAAATGAA+GGG 0.540164 3.3:-94262708 MS.gene064843:intron
AATTTGGGCAACTGTGGGAT+GGG 0.541935 3.3:+94261460 None:intergenic
GTGGCATAAGAACTACATCC+AGG 0.542673 3.3:-94263889 MS.gene064843:CDS
TGGACAAATTTGAAATTGAG+TGG 0.544484 3.3:-94264018 MS.gene064843:CDS
ATTAAACACACCATTACCGT+TGG 0.550414 3.3:+94265116 None:intergenic
GTGCGTGAAGGCCATCAACA+AGG 0.552340 3.3:+94264677 None:intergenic
ACACCCAGATGATTATAAAG+AGG 0.558171 3.3:-94263264 MS.gene064843:CDS
AATGTCCAGAGTCAAGATCA+AGG 0.560616 3.3:-94262545 MS.gene064843:intron
TAGGAACGAAATGAGCATAA+CGG 0.561873 3.3:+94264731 None:intergenic
CCTGCAATTTGGGCAACTGT+GGG 0.564136 3.3:+94261455 None:intergenic
TGATTATAAAGAGGCTATGG+AGG 0.565059 3.3:-94263255 MS.gene064843:CDS
TGTAACTTTGTTGTCAACAC+AGG 0.566833 3.3:+94263725 None:intergenic
GTTTAATGAAGAAGGAAAAG+AGG 0.567512 3.3:-94265100 MS.gene064843:CDS
ATAGGATCACCAATAACAGA+TGG 0.568307 3.3:+94263167 None:intergenic
TCTAAATGAAGTCAATGAAG+AGG 0.568616 3.3:-94263018 MS.gene064843:CDS
TAGCCTCTTTATAATCATCT+GGG 0.570856 3.3:+94263261 None:intergenic
GTTGATGGCCTTCACGCACA+CGG 0.573850 3.3:-94264673 MS.gene064843:CDS
ATTTGAAATTGAGTGGAAGA+GGG 0.577779 3.3:-94264011 MS.gene064843:CDS
AACGGGTTGGAGTTAACTCA+TGG 0.579913 3.3:+94264749 None:intergenic
CTACTTATACAATGAAGGAA+AGG 0.581665 3.3:-94264563 MS.gene064843:CDS
ATGTAAACAAAGATGATGAT+GGG 0.584402 3.3:-94264942 MS.gene064843:CDS
GTGATTGTAGACAGTTTGAA+CGG 0.585179 3.3:-94263502 MS.gene064843:CDS
TTCTAACACCGTGTGCGTGA+AGG 0.586649 3.3:+94264665 None:intergenic
CAATTTATCACACAATACAT+GGG 0.590150 3.3:-94263975 MS.gene064843:CDS
AGTTAACTCATGGTTGTGTG+AGG 0.591294 3.3:+94264759 None:intergenic
GATTATAAAGAGGCTATGGA+GGG 0.593735 3.3:-94263254 MS.gene064843:CDS
TACTCACAGTTGTATGATGA+GGG 0.598663 3.3:+94262255 None:intergenic
AGAACTACATCCAGGTGTGA+AGG 0.599918 3.3:-94263881 MS.gene064843:CDS
TCGATTAGTATGTAATAGAG+AGG 0.600970 3.3:-94264905 MS.gene064843:CDS
ATAAAGAGGCGTCGTGAGAG+TGG 0.603066 3.3:-94263209 MS.gene064843:CDS
ACGAAATGAGCATAACGGGT+TGG 0.604271 3.3:+94264736 None:intergenic
ATTTGTGTGAGAAAATAAAG+AGG 0.607012 3.3:-94263223 MS.gene064843:CDS
TTTATGCTGGCTCAGAAAGG+AGG 0.607302 3.3:-94264625 MS.gene064843:CDS
TGTTGACAACAAAGTTACAC+AGG 0.607980 3.3:-94263721 MS.gene064843:CDS
AATGGTGAGGATTTGGCCAA+CGG 0.609576 3.3:-94265132 MS.gene064843:CDS
CCTATCAAAGCCAAGGCCAA+AGG 0.609665 3.3:-94263149 MS.gene064843:CDS
AAAGGAGGTCATGAGAGTCT+GGG 0.612373 3.3:-94264610 MS.gene064843:CDS
TGGAAGACCAAAATGTAACA+TGG 0.612794 3.3:-94262493 MS.gene064843:CDS
GTGTTGATAAGAGTTGTGAC+AGG 0.616075 3.3:-94262864 MS.gene064843:CDS
TGGTGATCCTATCAAAGCCA+AGG 0.618217 3.3:-94263156 MS.gene064843:CDS
AAAAGGACATGATAACGAGT+TGG 0.619240 3.3:+94261529 None:intergenic
GTAAGAAGAAGAAAACATCC+AGG 0.620639 3.3:-94263118 MS.gene064843:CDS
GATGGGAAGTTGTCTGCTCG+TGG 0.621816 3.3:+94262317 None:intergenic
GTTAACTCATGGTTGTGTGA+GGG 0.630795 3.3:+94264760 None:intergenic
CGATGGAGACATAAATGGTG+AGG 0.630801 3.3:-94265145 MS.gene064843:CDS
ATACTCACAGTTGTATGATG+AGG 0.634928 3.3:+94262254 None:intergenic
CATATGAGATGAAGACAATG+TGG 0.635222 3.3:-94263943 MS.gene064843:CDS
CGATTAGTATGTAATAGAGA+GGG 0.642038 3.3:-94264904 MS.gene064843:CDS
GCACAAATAATATGGGAACA+TGG 0.643217 3.3:+94263470 None:intergenic
TGCGTGAAGGCCATCAACAA+GGG 0.643321 3.3:+94264678 None:intergenic
AAGGTGGACCAAATCAGCAA+AGG 0.645642 3.3:-94263399 MS.gene064843:CDS
GATTTATGCTAATTATACCG+TGG 0.646515 3.3:-94264038 MS.gene064843:CDS
ATTATAAAGAGGCTATGGAG+GGG 0.655805 3.3:-94263253 MS.gene064843:CDS
TTCGAACTAAGTGTTCTGCA+AGG 0.659792 3.3:-94264837 MS.gene064843:CDS
AAAATGTTCATGTTTCGTCG+AGG 0.666687 3.3:-94263332 MS.gene064843:CDS
TATGTTCTCAGGGTACCAGT+AGG 0.668100 3.3:-94261552 MS.gene064843:intron
ATATGAGATGAAGACAATGT+GGG 0.670288 3.3:-94263942 MS.gene064843:CDS
ATAATTAGCATAAATCAATG+AGG 0.681924 3.3:+94264045 None:intergenic
TGTCTGCTCGTGGAAATTGG+GGG 0.731356 3.3:+94262327 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATGTTGATACTAATAAA+TGG - chr3.3:94261778-94261797 MS.gene064843:intron 15.0%
!!! TAGTAATTTTATTGAATAAC+AGG - chr3.3:94264908-94264927 MS.gene064843:CDS 15.0%
!!! TTGTTCATTTTTTTTGTTTT+AGG - chr3.3:94264614-94264633 MS.gene064843:CDS 15.0%
!!! TTTAGAAAATTTATTTTGGT+CGG - chr3.3:94262145-94262164 MS.gene064843:intron 15.0%
!! AAAATGGATAAGAATATATG+TGG - chr3.3:94264466-94264485 MS.gene064843:intron 20.0%
!! ATAATTAGCATAAATCAATG+AGG + chr3.3:94262537-94262556 None:intergenic 20.0%
!! CAATAAAAGAATATCAAAAC+AGG - chr3.3:94263785-94263804 MS.gene064843:CDS 20.0%
!! TATAACTACTTATACAATGA+AGG - chr3.3:94262011-94262030 MS.gene064843:intron 20.0%
!!! AAGTTTAAGATGAATAGATT+TGG - chr3.3:94262998-94263017 MS.gene064843:CDS 20.0%
!!! AATTATAATTTGTTTGCACA+GGG - chr3.3:94263693-94263712 MS.gene064843:CDS 20.0%
!!! ACAATTTTATGAAATCTATG+CGG - chr3.3:94261584-94261603 MS.gene064843:intron 20.0%
!!! GACGTTTAGAAAATTTATTT+TGG - chr3.3:94262141-94262160 MS.gene064843:intron 20.0%
!!! TAATTATAATTTGTTTGCAC+AGG - chr3.3:94263692-94263711 MS.gene064843:CDS 20.0%
!!! TATGTTCCTAATTATTTTAC+CGG - chr3.3:94264124-94264143 MS.gene064843:intron 20.0%
!!! TTAGAGTATTTAAAGTTTGA+AGG - chr3.3:94264758-94264777 MS.gene064843:CDS 20.0%
! AAAAATGTCAAAACAGACAT+TGG + chr3.3:94264585-94264604 None:intergenic 25.0%
! AAAGTAGATGCAATTTGATT+TGG + chr3.3:94264196-94264215 None:intergenic 25.0%
! ACAATTTATCACACAATACA+TGG - chr3.3:94262603-94262622 MS.gene064843:intron 25.0%
! AGTAGTTTCTCATAGTTAAT+TGG - chr3.3:94263954-94263973 MS.gene064843:CDS 25.0%
! ATGTAAACAAAGATGATGAT+GGG - chr3.3:94261637-94261656 MS.gene064843:intron 25.0%
! ATTAAATCAGTTTAAGCCAT+TGG - chr3.3:94264836-94264855 MS.gene064843:CDS 25.0%
! ATTTGTGTGAGAAAATAAAG+AGG - chr3.3:94263356-94263375 MS.gene064843:CDS 25.0%
! CAATTTATCACACAATACAT+GGG - chr3.3:94262604-94262623 MS.gene064843:intron 25.0%
! CACAACTAAATTAAGTATCA+TGG + chr3.3:94265003-94265022 None:intergenic 25.0%
! CATAAAACCAAAAATATGAC+AGG + chr3.3:94261940-94261959 None:intergenic 25.0%
! TGTACTAGATTGAAAATGAA+GGG - chr3.3:94263871-94263890 MS.gene064843:CDS 25.0%
! TTGTACTAGATTGAAAATGA+AGG - chr3.3:94263870-94263889 MS.gene064843:CDS 25.0%
!! GTAAACTTTGAAAAGAACAT+TGG + chr3.3:94262107-94262126 None:intergenic 25.0%
!! GTATCTAGTTTATCTTGATA+AGG - chr3.3:94263036-94263055 MS.gene064843:CDS 25.0%
!! TCTCTATTTATGTTAGTTTG+TGG - chr3.3:94264417-94264436 MS.gene064843:intron 25.0%
!! TTATCTGTCATGTTTTCATT+AGG + chr3.3:94263906-94263925 None:intergenic 25.0%
!!! AAGATACTTGATACTTTTTG+TGG - chr3.3:94263814-94263833 MS.gene064843:CDS 25.0%
!!! CTCTATTTATGTTAGTTTGT+GGG - chr3.3:94264418-94264437 MS.gene064843:intron 25.0%
!!! TCATATTTTTGGTTTTATGC+TGG - chr3.3:94261941-94261960 MS.gene064843:intron 25.0%
AAAACAGACATTGGAACAAA+TGG + chr3.3:94264576-94264595 None:intergenic 30.0%
AAAATTGATGCACCTTGATT+TGG + chr3.3:94264232-94264251 None:intergenic 30.0%
AAGCTTCAAAAATCTTTACG+AGG - chr3.3:94262891-94262910 MS.gene064843:intron 30.0%
AATCGATTGAAAGTAACTTC+AGG + chr3.3:94264784-94264803 None:intergenic 30.0%
AATGGTGTGTTTAATGAAGA+AGG - chr3.3:94261471-94261490 MS.gene064843:CDS 30.0%
ACTAATAAATGGAGAGTTTC+TGG - chr3.3:94261789-94261808 MS.gene064843:intron 30.0%
ACTGCAATTATCTATTCCAA+TGG + chr3.3:94264855-94264874 None:intergenic 30.0%
AGAAACAAAGTACATATTCG+AGG - chr3.3:94261712-94261731 MS.gene064843:intron 30.0%
AGATGATTATAAAGAGGCTA+TGG - chr3.3:94263321-94263340 MS.gene064843:CDS 30.0%
AGTTGTGAAAATATGTTCTC+AGG - chr3.3:94265016-94265035 MS.gene064843:CDS 30.0%
ATAGAGCTGTAAAAGAATAC+AGG - chr3.3:94262789-94262808 MS.gene064843:intron 30.0%
ATAGCCTCTTTATAATCATC+TGG + chr3.3:94263322-94263341 None:intergenic 30.0%
ATATGAGATGAAGACAATGT+GGG - chr3.3:94262637-94262656 MS.gene064843:intron 30.0%
ATGTATCATAAACAGCCTAA+AGG - chr3.3:94262371-94262390 MS.gene064843:CDS 30.0%
CAAAAACACATTTAACACGA+TGG + chr3.3:94263642-94263661 None:intergenic 30.0%
CAAAATGTAACATGGAAAGA+TGG - chr3.3:94264094-94264113 MS.gene064843:intron 30.0%
CGATTAGTATGTAATAGAGA+GGG - chr3.3:94261675-94261694 MS.gene064843:intron 30.0%
CTACTTATACAATGAAGGAA+AGG - chr3.3:94262016-94262035 MS.gene064843:intron 30.0%
CTCAATTTCAAATTTGTCCA+CGG + chr3.3:94262561-94262580 None:intergenic 30.0%
GATGTAAACAAAGATGATGA+TGG - chr3.3:94261636-94261655 MS.gene064843:intron 30.0%
GATTGCAAATGAAAACCATA+TGG + chr3.3:94264942-94264961 None:intergenic 30.0%
GATTTATGCTAATTATACCG+TGG - chr3.3:94262541-94262560 MS.gene064843:intron 30.0%
GGATACTTACAAGAAGATTA+GGG - chr3.3:94261506-94261525 MS.gene064843:CDS 30.0%
GTTGTGAAAATATGTTCTCA+GGG - chr3.3:94265017-94265036 MS.gene064843:CDS 30.0%
GTTTAATGAAGAAGGAAAAG+AGG - chr3.3:94261479-94261498 MS.gene064843:CDS 30.0%
TAGATTGAAAATGAAGGGAT+TGG - chr3.3:94263876-94263895 MS.gene064843:CDS 30.0%
TAGCCTCTTTATAATCATCT+GGG + chr3.3:94263321-94263340 None:intergenic 30.0%
TATCAGATTCACTCAATTGA+CGG + chr3.3:94261878-94261897 None:intergenic 30.0%
TCGATTAGTATGTAATAGAG+AGG - chr3.3:94261674-94261693 MS.gene064843:intron 30.0%
TCTAAATGAAGTCAATGAAG+AGG - chr3.3:94263561-94263580 MS.gene064843:CDS 30.0%
TCTCATGGCACAAATAATAT+GGG + chr3.3:94263119-94263138 None:intergenic 30.0%
TGGATACTTACAAGAAGATT+AGG - chr3.3:94261505-94261524 MS.gene064843:CDS 30.0%
TGTATCATAAACAGCCTAAA+GGG - chr3.3:94262372-94262391 MS.gene064843:CDS 30.0%
TGTGAAGAATCCAAAATTCT+TGG - chr3.3:94262502-94262521 MS.gene064843:CDS 30.0%
TTTGGAAATTGAGGAAAGAT+CGG + chr3.3:94264178-94264197 None:intergenic 30.0%
! AATTTGAAATTGAGTGGAAG+AGG - chr3.3:94262567-94262586 MS.gene064843:intron 30.0%
! ATAGTCTAATTTGCAAAAGG+TGG - chr3.3:94263164-94263183 MS.gene064843:CDS 30.0%
! ATGTTTTCTTCTTCTTACGA+GGG + chr3.3:94263459-94263478 None:intergenic 30.0%
! ATTTGAAATTGAGTGGAAGA+GGG - chr3.3:94262568-94262587 MS.gene064843:intron 30.0%
! CATCTTTCCATGTTACATTT+TGG + chr3.3:94264096-94264115 None:intergenic 30.0%
! CTGATAGTCTAATTTGCAAA+AGG - chr3.3:94263161-94263180 MS.gene064843:CDS 30.0%
! CTTTTGCAAATTAGACTATC+AGG + chr3.3:94263163-94263182 None:intergenic 30.0%
! GAAAAGTCTTCCAAGAATTT+TGG + chr3.3:94262515-94262534 None:intergenic 30.0%
! GTTAGAACCTGTCATATTTT+TGG - chr3.3:94261930-94261949 MS.gene064843:intron 30.0%
! TGGACAAATTTGAAATTGAG+TGG - chr3.3:94262561-94262580 MS.gene064843:intron 30.0%
!! ATAATTTTGAGTCGTGCAAA+AGG - chr3.3:94264546-94264565 MS.gene064843:intron 30.0%
!! CCTTGATTTGGAAAAAATTG+TGG + chr3.3:94264220-94264239 None:intergenic 30.0%
!! GCAATTTGATTTGGAAATTG+AGG + chr3.3:94264187-94264206 None:intergenic 30.0%
!! TAACAGGTTTGTTAACCATA+TGG - chr3.3:94264924-94264943 MS.gene064843:CDS 30.0%
!! TTTGGTCTTCCATTTTAACA+GGG + chr3.3:94264078-94264097 None:intergenic 30.0%
!! TTTTGGTCTTCCATTTTAAC+AGG + chr3.3:94264079-94264098 None:intergenic 30.0%
!!! AAGCCTCTTGTTATTTTTTC+TGG - chr3.3:94262248-94262267 MS.gene064843:intron 30.0%
!!! AGCCTCTTGTTATTTTTTCT+GGG - chr3.3:94262249-94262268 MS.gene064843:intron 30.0%
!!! ATGCGAGAAAACTTTTTTTG+TGG - chr3.3:94262671-94262690 MS.gene064843:CDS 30.0%
!!! CCACAATTTTTTCCAAATCA+AGG - chr3.3:94264217-94264236 MS.gene064843:intron 30.0%
AAAAGCCAATCTCATCCAAA+AGG + chr3.3:94265070-94265089 None:intergenic 35.0%
AAAATGTTCATGTTTCGTCG+AGG - chr3.3:94263247-94263266 MS.gene064843:CDS 35.0%
AAATTGTAGTTCCTCCATGA+TGG + chr3.3:94262280-94262299 None:intergenic 35.0%
AAGACCAATCCATCTGTTAT+TGG - chr3.3:94263403-94263422 MS.gene064843:CDS 35.0%
AAGATCGGAACACTTTGAAT+TGG + chr3.3:94264163-94264182 None:intergenic 35.0%
AAGTTTACCACAACAGAAGA+AGG - chr3.3:94262119-94262138 MS.gene064843:intron 35.0%
AATGAGAGAAGCTATCAAAG+AGG - chr3.3:94262418-94262437 MS.gene064843:CDS 35.0%
ACACCCAGATGATTATAAAG+AGG - chr3.3:94263315-94263334 MS.gene064843:CDS 35.0%
ACAGATAAAAACATGCACAC+AGG - chr3.3:94263918-94263937 MS.gene064843:CDS 35.0%
AGAAGAAAACATCCAGGAAA+AGG - chr3.3:94263467-94263486 MS.gene064843:CDS 35.0%
AGACATAAATGGTGAGGATT+TGG - chr3.3:94261440-94261459 MS.gene064843:CDS 35.0%
AGGATTGGAATTTGATTCAG+AGG - chr3.3:94261551-94261570 MS.gene064843:intron 35.0%
ATACAGGCACAAAGAGTTAA+TGG - chr3.3:94262805-94262824 MS.gene064843:CDS 35.0%
ATACTCACAGTTGTATGATG+AGG + chr3.3:94264328-94264347 None:intergenic 35.0%
ATAGGATCACCAATAACAGA+TGG + chr3.3:94263415-94263434 None:intergenic 35.0%
ATCACCAATAACAGATGGAT+TGG + chr3.3:94263410-94263429 None:intergenic 35.0%
ATTAAACACACCATTACCGT+TGG + chr3.3:94261466-94261485 None:intergenic 35.0%
ATTATAAAGAGGCTATGGAG+GGG - chr3.3:94263326-94263345 MS.gene064843:CDS 35.0%
ATTTATGTCTCCATCGTTTG+CGG + chr3.3:94261430-94261449 None:intergenic 35.0%
CAAAACACTCATAGTCAATC+CGG + chr3.3:94262181-94262200 None:intergenic 35.0%
CAACAGAACTTGAATGTGAT+GGG + chr3.3:94264282-94264301 None:intergenic 35.0%
CAAGAAGATTAGGGAATTGA+CGG - chr3.3:94261515-94261534 MS.gene064843:CDS 35.0%
CACCCAGAAAAAATAACAAG+AGG + chr3.3:94262254-94262273 None:intergenic 35.0%
CAGCAGTTAAAGCAACAAAT+AGG + chr3.3:94264655-94264674 None:intergenic 35.0%
CATATGAGATGAAGACAATG+TGG - chr3.3:94262636-94262655 MS.gene064843:intron 35.0%
GATGGAACGATTAGCAAAAA+TGG - chr3.3:94264450-94264469 MS.gene064843:intron 35.0%
GATTATAAAGAGGCTATGGA+GGG - chr3.3:94263325-94263344 MS.gene064843:CDS 35.0%
GCACAAATAATATGGGAACA+TGG + chr3.3:94263112-94263131 None:intergenic 35.0%
GGATGATGATATAAGAGGAT+TGG - chr3.3:94261536-94261555 MS.gene064843:CDS 35.0%
GTAAGAAGAAGAAAACATCC+AGG - chr3.3:94263461-94263480 MS.gene064843:CDS 35.0%
GTATCATAAACAGCCTAAAG+GGG - chr3.3:94262373-94262392 MS.gene064843:CDS 35.0%
GTCTCATGGCACAAATAATA+TGG + chr3.3:94263120-94263139 None:intergenic 35.0%
GTGATTGTAGACAGTTTGAA+CGG - chr3.3:94263077-94263096 MS.gene064843:CDS 35.0%
TAACTGCTGAAAATCATGAG+TGG - chr3.3:94264666-94264685 MS.gene064843:CDS 35.0%
TACTCACAGTTGTATGATGA+GGG + chr3.3:94264327-94264346 None:intergenic 35.0%
TAGGAACGAAATGAGCATAA+CGG + chr3.3:94261851-94261870 None:intergenic 35.0%
TATTATTTGTGCCATGAGAC+AGG - chr3.3:94263120-94263139 MS.gene064843:CDS 35.0%
TGAAGAAGGAAAAGAGGATA+TGG - chr3.3:94261485-94261504 MS.gene064843:CDS 35.0%
TGATTATAAAGAGGCTATGG+AGG - chr3.3:94263324-94263343 MS.gene064843:CDS 35.0%
TGGAAGACCAAAATGTAACA+TGG - chr3.3:94264086-94264105 MS.gene064843:intron 35.0%
TGTTGAAAGCAAAAACAGTC+TGG - chr3.3:94262745-94262764 MS.gene064843:intron 35.0%
TGTTGACAACAAAGTTACAC+AGG - chr3.3:94262858-94262877 MS.gene064843:CDS 35.0%
! AAAAGGACATGATAACGAGT+TGG + chr3.3:94265053-94265072 None:intergenic 35.0%
! AAATCTATGCGGATTGTCAT+GGG - chr3.3:94261595-94261614 MS.gene064843:intron 35.0%
! AACTCGTTATCATGTCCTTT+TGG - chr3.3:94265052-94265071 MS.gene064843:CDS 35.0%
! CATGGAGGAACTACAATTTT+TGG - chr3.3:94262281-94262300 MS.gene064843:CDS 35.0%
! CATTCAAGTTCTGTTGGATA+TGG - chr3.3:94264286-94264305 MS.gene064843:intron 35.0%
! CTTTTTGCGTGCAATTATCA+CGG - chr3.3:94264962-94264981 MS.gene064843:CDS 35.0%
! GATGTTTTCTTCTTCTTACG+AGG + chr3.3:94263460-94263479 None:intergenic 35.0%
! GATTGACTATGAGTGTTTTG+GGG - chr3.3:94262181-94262200 MS.gene064843:intron 35.0%
! GGATTGACTATGAGTGTTTT+GGG - chr3.3:94262180-94262199 MS.gene064843:intron 35.0%
! GTAATCATCCTTTGCTGATT+TGG + chr3.3:94263191-94263210 None:intergenic 35.0%
! TGTAACTTTGTTGTCAACAC+AGG + chr3.3:94262857-94262876 None:intergenic 35.0%
! TGTTTTCTTCTTCTTACGAG+GGG + chr3.3:94263458-94263477 None:intergenic 35.0%
! TTGACGGATGATGATATAAG+AGG - chr3.3:94261531-94261550 MS.gene064843:CDS 35.0%
!! TTGGTCTTCCATTTTAACAG+GGG + chr3.3:94264077-94264096 None:intergenic 35.0%
AAACACGTGAGACATACAAG+TGG - chr3.3:94262324-94262343 MS.gene064843:CDS 40.0%
AAAGGCAAGAATAGAAGGTG+GGG - chr3.3:94262034-94262053 MS.gene064843:intron 40.0%
AACTCTCGAATCACAAGCAA+TGG - chr3.3:94264503-94264522 MS.gene064843:intron 40.0%
AATGTCCAGAGTCAAGATCA+AGG - chr3.3:94264034-94264053 MS.gene064843:CDS 40.0%
ACTTTGAATTGGAACACCGT+TGG + chr3.3:94264152-94264171 None:intergenic 40.0%
AGGAACGAAATGAGCATAAC+GGG + chr3.3:94261850-94261869 None:intergenic 40.0%
AGTTAACTCATGGTTGTGTG+AGG + chr3.3:94261823-94261842 None:intergenic 40.0%
CAAAGACGATGCAAAGAGTT+TGG + chr3.3:94262449-94262468 None:intergenic 40.0%
CCAACAGAACTTGAATGTGA+TGG + chr3.3:94264283-94264302 None:intergenic 40.0%
CGTGATAATTGCACGCAAAA+AGG + chr3.3:94264964-94264983 None:intergenic 40.0%
CTTTGCATCGTCTTTGTTCA+TGG - chr3.3:94262453-94262472 MS.gene064843:CDS 40.0%
GAAAGGCAAGAATAGAAGGT+GGG - chr3.3:94262033-94262052 MS.gene064843:intron 40.0%
GAAGGAAAGGCAAGAATAGA+AGG - chr3.3:94262029-94262048 MS.gene064843:intron 40.0%
GCAAACGATGGAGACATAAA+TGG - chr3.3:94261429-94261448 MS.gene064843:CDS 40.0%
GTTAACTCATGGTTGTGTGA+GGG + chr3.3:94261822-94261841 None:intergenic 40.0%
TAAACGTCCTTCTTCTGTTG+TGG + chr3.3:94262129-94262148 None:intergenic 40.0%
TCACTCAATTGACGGTTCTT+AGG + chr3.3:94261870-94261889 None:intergenic 40.0%
TCTGACCATGACAAAAACGT+TGG - chr3.3:94263598-94263617 MS.gene064843:CDS 40.0%
TCTGATAAAGCCCTTGTTGA+TGG - chr3.3:94261891-94261910 MS.gene064843:intron 40.0%
TGACAAAAACGTTGGATGTG+AGG - chr3.3:94263606-94263625 MS.gene064843:CDS 40.0%
TGATAACCCCCCTGTTAAAA+TGG - chr3.3:94264066-94264085 MS.gene064843:intron 40.0%
TGCAGAACACTTAGTTCGAA+TGG + chr3.3:94261742-94261761 None:intergenic 40.0%
TGTAGACAGTTTGAACGGAT+TGG - chr3.3:94263082-94263101 MS.gene064843:CDS 40.0%
TTCGAACTAAGTGTTCTGCA+AGG - chr3.3:94261742-94261761 MS.gene064843:intron 40.0%
TTGCATTTGACGCAACATAC+AGG - chr3.3:94262210-94262229 MS.gene064843:intron 40.0%
TTGCTTGTGATTCGAGAGTT+TGG + chr3.3:94264503-94264522 None:intergenic 40.0%
! AGAACAATGTCTCCTTTTCC+TGG + chr3.3:94263482-94263501 None:intergenic 40.0%
! AGTCTGGTTGATTTCATGCA+TGG - chr3.3:94262761-94262780 MS.gene064843:intron 40.0%
! CACATCCAACGTTTTTGTCA+TGG + chr3.3:94263606-94263625 None:intergenic 40.0%
! CATGTCCTTTTGGATGAGAT+TGG - chr3.3:94265062-94265081 MS.gene064843:CDS 40.0%
! CCATCACATTCAAGTTCTGT+TGG - chr3.3:94264280-94264299 MS.gene064843:intron 40.0%
! CGGATTGACTATGAGTGTTT+TGG - chr3.3:94262179-94262198 MS.gene064843:intron 40.0%
! GAAATCTATGCGGATTGTCA+TGG - chr3.3:94261594-94261613 MS.gene064843:intron 40.0%
! GTGTTGATAAGAGTTGTGAC+AGG - chr3.3:94263715-94263734 MS.gene064843:CDS 40.0%
! TTTTCTGGGTGTAACCATCA+TGG - chr3.3:94262263-94262282 MS.gene064843:CDS 40.0%
!! AATTATTTTACCGGCGCCAA+CGG - chr3.3:94264133-94264152 MS.gene064843:intron 40.0%
!! GTTTTTGAGCAGTCTAGTCT+CGG - chr3.3:94262965-94262984 MS.gene064843:intron 40.0%
!! TGGTCTTCCATTTTAACAGG+GGG + chr3.3:94264076-94264095 None:intergenic 40.0%
!! TTGGCGCCGGTAAAATAATT+AGG + chr3.3:94264133-94264152 None:intergenic 40.0%
!!! TTGGATGAGATTGGCTTTTC+TGG - chr3.3:94265071-94265090 MS.gene064843:CDS 40.0%
AAAGGAGGTCATGAGAGTCT+GGG - chr3.3:94261969-94261988 MS.gene064843:intron 45.0%
AACATCACGTCCAGCTAGTT+CGG + chr3.3:94263542-94263561 None:intergenic 45.0%
AAGGTGGACCAAATCAGCAA+AGG - chr3.3:94263180-94263199 MS.gene064843:CDS 45.0%
AATGGTGAGGATTTGGCCAA+CGG - chr3.3:94261447-94261466 MS.gene064843:CDS 45.0%
AATTTGGGCAACTGTGGGAT+GGG + chr3.3:94265122-94265141 None:intergenic 45.0%
AGAACTACATCCAGGTGTGA+AGG - chr3.3:94262698-94262717 MS.gene064843:CDS 45.0%
AGTTGTCTGCTCGTGGAAAT+TGG + chr3.3:94264258-94264277 None:intergenic 45.0%
CAACACCTTGATCTTGACTC+TGG + chr3.3:94264042-94264061 None:intergenic 45.0%
CAGACACTGTCCTGCAATTT+GGG + chr3.3:94265137-94265156 None:intergenic 45.0%
CCATCAGTAACTACCCCTTT+AGG + chr3.3:94262389-94262408 None:intergenic 45.0%
CCTAAAGGGGTAGTTACTGA+TGG - chr3.3:94262386-94262405 MS.gene064843:CDS 45.0%
CGATGGAGACATAAATGGTG+AGG - chr3.3:94261434-94261453 MS.gene064843:CDS 45.0%
GGAAAGGCAAGAATAGAAGG+TGG - chr3.3:94262032-94262051 MS.gene064843:intron 45.0%
GTGGCATAAGAACTACATCC+AGG - chr3.3:94262690-94262709 MS.gene064843:CDS 45.0%
GTTGTCTGCTCGTGGAAATT+GGG + chr3.3:94264257-94264276 None:intergenic 45.0%
TGCTAATCGTTCCATCTCCA+AGG + chr3.3:94264446-94264465 None:intergenic 45.0%
TGCTGCGTTGAGTTATTTGC+AGG - chr3.3:94262064-94262083 MS.gene064843:intron 45.0%
TGGTGATCCTATCAAAGCCA+AGG - chr3.3:94263423-94263442 MS.gene064843:CDS 45.0%
TTGTCTGCTCGTGGAAATTG+GGG + chr3.3:94264256-94264275 None:intergenic 45.0%
! ATGATAACGAGTTGGCCTAC+TGG + chr3.3:94265045-94265064 None:intergenic 45.0%
! TATGTTCTCAGGGTACCAGT+AGG - chr3.3:94265027-94265046 MS.gene064843:CDS 45.0%
!! AACGGGTTGGAGTTAACTCA+TGG + chr3.3:94261833-94261852 None:intergenic 45.0%
!! ACGAAATGAGCATAACGGGT+TGG + chr3.3:94261846-94261865 None:intergenic 45.0%
!! ATGAGAGTCTGGGATTTTGC+AGG - chr3.3:94261979-94261998 MS.gene064843:intron 45.0%
!! ATGTTAGTTTGTGGGTGCCT+TGG - chr3.3:94264426-94264445 MS.gene064843:intron 45.0%
!! GGTCTTCCATTTTAACAGGG+GGG + chr3.3:94264075-94264094 None:intergenic 45.0%
!! TTTATGCTGGCTCAGAAAGG+AGG - chr3.3:94261954-94261973 MS.gene064843:intron 45.0%
!!! CTATGAGTGTTTTGGGGATG+TGG - chr3.3:94262187-94262206 MS.gene064843:intron 45.0%
!!! GGTTTTATGCTGGCTCAGAA+AGG - chr3.3:94261951-94261970 MS.gene064843:intron 45.0%
ACACTCATAGTCAATCCGGC+TGG + chr3.3:94262177-94262196 None:intergenic 50.0%
ACATCACGTCCAGCTAGTTC+GGG + chr3.3:94263541-94263560 None:intergenic 50.0%
AGTCATGTGCTCCTGTCTCA+TGG + chr3.3:94263134-94263153 None:intergenic 50.0%
CAAAACTGTCCCGAACTAGC+TGG - chr3.3:94263529-94263548 MS.gene064843:CDS 50.0%
CAATTTGGGCAACTGTGGGA+TGG + chr3.3:94265123-94265142 None:intergenic 50.0%
CCTATCAAAGCCAAGGCCAA+AGG - chr3.3:94263430-94263449 MS.gene064843:CDS 50.0%
CCTGCAATTTGGGCAACTGT+GGG + chr3.3:94265127-94265146 None:intergenic 50.0%
CGAGTTTATGCCTTCACACC+TGG + chr3.3:94262711-94262730 None:intergenic 50.0%
GAAAGGAGGTCATGAGAGTC+TGG - chr3.3:94261968-94261987 MS.gene064843:intron 50.0%
GAGGATTTGGCCAACGGTAA+TGG - chr3.3:94261453-94261472 MS.gene064843:CDS 50.0%
GCAGACACTGTCCTGCAATT+TGG + chr3.3:94265138-94265157 None:intergenic 50.0%
TCCTGCAATTTGGGCAACTG+TGG + chr3.3:94265128-94265147 None:intergenic 50.0%
TCTGGGTGTAACCATCATGG+AGG - chr3.3:94262266-94262285 MS.gene064843:CDS 50.0%
TGCGTGAAGGCCATCAACAA+GGG + chr3.3:94261904-94261923 None:intergenic 50.0%
TGTCTGCTCGTGGAAATTGG+GGG + chr3.3:94264255-94264274 None:intergenic 50.0%
TTCTAACACCGTGTGCGTGA+AGG + chr3.3:94261917-94261936 None:intergenic 50.0%
TTTGGTCGGATTCAACCAGC+CGG - chr3.3:94262159-94262178 MS.gene064843:intron 50.0%
! TGCGGATTGTCATGGGTTTG+TGG - chr3.3:94261602-94261621 MS.gene064843:intron 50.0%
!! ATAAAGAGGCGTCGTGAGAG+TGG - chr3.3:94263370-94263389 MS.gene064843:CDS 50.0%
!! CCTTTGGCCTTGGCTTTGAT+AGG + chr3.3:94263433-94263452 None:intergenic 50.0%
!! GAGTCTGGGATTTTGCAGGA+AGG - chr3.3:94261983-94262002 MS.gene064843:intron 50.0%
AATTGGAACACCGTTGGCGC+CGG + chr3.3:94264146-94264165 None:intergenic 55.0%
CCCACAGTTGCCCAAATTGC+AGG - chr3.3:94265124-94265143 MS.gene064843:CDS 55.0%
GATGGGAAGTTGTCTGCTCG+TGG + chr3.3:94264265-94264284 None:intergenic 55.0%
GTGCGTGAAGGCCATCAACA+AGG + chr3.3:94261905-94261924 None:intergenic 55.0%
GTTTGTGGGTGCCTTGGAGA+TGG - chr3.3:94264432-94264451 MS.gene064843:intron 55.0%
!! CTTCTTACGAGGGGCACCTT+TGG + chr3.3:94263449-94263468 None:intergenic 55.0%
!! GTTGATGGCCTTCACGCACA+CGG - chr3.3:94261906-94261925 MS.gene064843:intron 55.0%
GGGCAACTGTGGGATGGGTG+AGG + chr3.3:94265117-94265136 None:intergenic 65.0%
!! ACGAGGGGCACCTTTGGCCT+TGG + chr3.3:94263443-94263462 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 94261423 94265178 94261423 ID=MS.gene064843
chr3.3 mRNA 94261423 94265178 94261423 ID=MS.gene064843.t1;Parent=MS.gene064843
chr3.3 exon 94264551 94265178 94264551 ID=MS.gene064843.t1.exon1;Parent=MS.gene064843.t1
chr3.3 CDS 94264551 94265178 94264551 ID=cds.MS.gene064843.t1;Parent=MS.gene064843.t1
chr3.3 exon 94262974 94264073 94262974 ID=MS.gene064843.t1.exon2;Parent=MS.gene064843.t1
chr3.3 CDS 94262974 94264073 94262974 ID=cds.MS.gene064843.t1;Parent=MS.gene064843.t1
chr3.3 exon 94262795 94262887 94262795 ID=MS.gene064843.t1.exon3;Parent=MS.gene064843.t1
chr3.3 CDS 94262795 94262887 94262795 ID=cds.MS.gene064843.t1;Parent=MS.gene064843.t1
chr3.3 exon 94262651 94262722 94262651 ID=MS.gene064843.t1.exon4;Parent=MS.gene064843.t1
chr3.3 CDS 94262651 94262722 94262651 ID=cds.MS.gene064843.t1;Parent=MS.gene064843.t1
chr3.3 exon 94262262 94262558 94262262 ID=MS.gene064843.t1.exon5;Parent=MS.gene064843.t1
chr3.3 CDS 94262262 94262558 94262262 ID=cds.MS.gene064843.t1;Parent=MS.gene064843.t1
chr3.3 exon 94261423 94261563 94261423 ID=MS.gene064843.t1.exon6;Parent=MS.gene064843.t1
chr3.3 CDS 94261423 94261563 94261423 ID=cds.MS.gene064843.t1;Parent=MS.gene064843.t1
Gene Sequence

>MS.gene064843

ATGGCCGCAAACGATGGAGACATAAATGGTGAGGATTTGGCCAACGGTAATGGTGTGTTTAATGAAGAAGGAAAAGAGGATATGGATACTTACAAGAAGATTAGGGAATTGACGGATGATGATATAAGAGGATTGGAATTTGATTCAGAGGAAAAAGCGACACAATTTTATGAAATCTATGCGGATTGTCATGGGTTTGTGGTAAGAAAAGATGATGTAAACAAAGATGATGATGGGAATATAATTGCACGTCGATTAGTATGTAATAGAGAGGGTGAGAATAAAAATAAGAAACAAAGTACATATTCGAGGCAGACCATTCGAACTAAGTGTTCTGCAAGGATTCGAGTAGTTTTAAATGTTGATACTAATAAATGGAGAGTTTCTGGATTCGAGCCCTCACACAACCATGAGTTAACTCCAACCCGTTATGCTCATTTCGTTCCTAAGAACCGTCAATTGAGTGAATCTGATAAAGCCCTTGTTGATGGCCTTCACGCACACGGTGTTAGAACCTGTCATATTTTTGGTTTTATGCTGGCTCAGAAAGGAGGTCATGAGAGTCTGGGATTTTGCAGGAAGGACTTATATAACTACTTATACAATGAAGGAAAGGCAAGAATAGAAGGTGGGGATTCTTTTGCTGCGTTGAGTTATTTGCAGGCTAAAGCTGATCATGATCCAATGTTCTTTTCAAAGTTTACCACAACAGAAGAAGGACGTTTAGAAAATTTATTTTGGTCGGATTCAACCAGCCGGATTGACTATGAGTGTTTTGGGGATGTGGTTGCATTTGACGCAACATACAGGAAGAATAGATATGACAAGCCTCTTGTTATTTTTTCTGGGTGTAACCATCATGGAGGAACTACAATTTTTGGTTGTGCTTTAGTTTCTAATGAAACACGTGAGACATACAAGTGGCTTTTAAATACATTTTTAGAAGCAATGTATCATAAACAGCCTAAAGGGGTAGTTACTGATGGAGATCTTTCAATGAGAGAAGCTATCAAAGAGGTATTTCCAAACTCTTTGCATCGTCTTTGTTCATGGCATTTTCATCGAAACGCTGAAGAGAATGTGAAGAATCCAAAATTCTTGGAAGACTTTTCCTCATTGATTTATGCTAATTATACCGTGGACAAATTTGAAATTGAGTGGAAGAGGGTCATTGATGATTACAATTTATCACACAATACATGGGTTAAAAAAGCATATGAGATGAAGACAATGTGGGCGAGTGCATACATGCGAGAAAACTTTTTTTGTGGCATAAGAACTACATCCAGGTGTGAAGGCATAAACTCGTTTATAAATATGTATGTTGAAAGCAAAAACAGTCTGGTTGATTTCATGCATGGTTTTAATAGAGCTGTAAAAGAATACAGGCACAAAGAGTTAATGGCAGATTTTAATACGTTCTATTACAAACCTGTGTTGACAACAAAGTTACACAGGTTTGAGCTTGAAGCTTCAAAAATCTTTACGAGGATAAAATTTTATGAAGTTCAAAATGAAATAGAAGATGCAATCACATTAAATGTTTTTGAGCAGTCTAGTCTCGGAAAGACTGTGAAGTTTAAGATGAATAGATTTGGCAACAAAGATTCTACGTATCTAGTTTATCTTGATAAGGAACAAAGTAAATTTTTATGTGATTGTAGACAGTTTGAACGGATTGGAATTCCATGTTCCCATATTATTTGTGCCATGAGACAGGAGCACATGACTGAGTTTCCTGATAGTCTAATTTGCAAAAGGTGGACCAAATCAGCAAAGGATGATTACATATCGTCTATGTCTTCTAAAGAATGCGATGATGAAAAAATGTTCATGTTTCGTCGAGGAACTATGTCTACTGCATTGAATAATCTATATGACATTTCTTGTAAACACCCAGATGATTATAAAGAGGCTATGGAGGGGATTTACAATTTGTGTGAGAAAATAAAGAGGCGTCGTGAGAGTGGTGAAAAATATAAGACCAATCCATCTGTTATTGGTGATCCTATCAAAGCCAAGGCCAAAGGTGCCCCTCGTAAGAAGAAGAAAACATCCAGGAAAAGGAGACATTGTTCTCTCTGCAAAAATATTGCTCACACGAAGCAAAACTGTCCCGAACTAGCTGGACGTGATGTTCTAAATGAAGTCAATGAAGAGGACTATTCTCGTCGATCTGACCATGACAAAAACGTTGGATGTGAGGTATTTTTACACCATCGTGTTAAATGTGTTTTTGTTTGTAAATGTTTGTGTTATTTATTCAATATAATTATAATTTGTTTGCACAGGGTGTTGATAAGAGTTGTGACAGGAAGCGTAAGAGAAAAGTTGTCAATGTTACTTCAATGCAAGAAGATATCAATAAAAGAATATCAAAACAGGTAATTGAAGATACTTGATACTTTTTGTGGAAATTTATTAAGTGTTTGACAACTTTTATAATGTTGTACTAGATTGAAAATGAAGGGATTGGTCGACCTAATGAAAACATGACAGATAAAAACATGCACACAGGATTTGACAACGTAAGTAGTTTCTCATAGTTAATTGGAGCAGATTTCAGTACTTTGATATTTTATTGCTGATGTTTTGATTTGTTTCAAAACAAAATGTCCAGAGTCAAGATCAAGGTGTTGTACATGATAACCCCCCTGTTAAAATGGAAGACCAAAATGTAACATGGAAAGATGGTTCAATGTATGTTCCTAATTATTTTACCGGCGCCAACGGTGTTCCAATTCAAAGTGTTCCGATCTTTCCTCAATTTCCAAATCAAATTGCATCTACTTTTCCACAATTTTTTCCAAATCAAGGTGCATCAATTTTCCCCCAATTTCCACGAGCAGACAACTTCCCATCACATTCAAGTTCTGTTGGATATGGCAGAGCACATAATGTCCCTCATCATACAACTGTGAGTATACACAACATTCATATTACTAATTATGTGAATTTATGCTGTCAAATATGTTTTCTCTCTCTAATATGTATTCTCTATTTATGTTAGTTTGTGGGTGCCTTGGAGATGGAACGATTAGCAAAAATGGATAAGAATATATGTGGAAATGAAGATTCCAAACTCTCGAATCACAAGCAATGGAACAAGCATCGAAAGTGATGATAATTTTGAGTCGTGCAAAAGGTTTACCATTTGTTCCAATGTCTGTTTTGACATTTTTTTATACTAATTGTTCATTTTTTTTGTTTTAGGTTTAACATATATCTTCCTATTTGTTGCTTTAACTGCTGAAAATCATGAGTGGAATGCTTAGATTGATTTGCTTGTTAGTTAGTTTAAAACTTACTTGATGATTGAAATGATAGTGTAATGCTTAGAGTATTTAAAGTTTGAAGGCCTGAAGTTACTTTCAATCGATTAATGTCAATTAGTTCATTCAATTTAATAGCTAATTAAATCAGTTTAAGCCATTGGAATAGATAATTGCAGTTACAGAATAATTTTAACTTCACTAGTAATTGTGTAGTAATTTTATTGAATAACAGGTTTGTTAACCATATGGTTTTCATTTGCAATCCTTTTTGCGTGCAATTATCACGGATTTCTATAGTAAAACCATGATACTTAATTTAGTTGTGAAAATATGTTCTCAGGGTACCAGTAGGCCAACTCGTTATCATGTCCTTTTGGATGAGATTGGCTTTTCTGGTAGCAGTCATGATATCAATGCCTCACCCATCCCACAGTTGCCCAAATTGCAGGACAGTGTCTGCAACTCTATGTTCTTTGTTTGA
Protein sequence

>MS.gene064843.t1

MAANDGDINGEDLANGNGVFNEEGKEDMDTYKKIRELTDDDIRGLEFDSEEKATQFYEIYADCHGFVVRKDDVNKDDDGNIIARRLVCNREGENKNKKQSTYSRQTIRTKCSARIRVVLNVDTNKWRVSGFEPSHNHELTPTRYAHFVPKNRQLSESDKALVDGLHAHGVRTCHIFGFMLAQKGGHESLGFCRKDLYNYLYNEGKARIEDFSSLIYANYTVDKFEIEWKRVIDDYNLSHNTWVKKAYEMKTMWASAYMRENFFCGIRTTSRCEGINSFINMYVESKNSLVDFMHGFNRAVKEYRHKELMADFNTFYYKPVLTTKLHRFELEASKIFTRIKFYEVQNEIEDAITLNVFEQSSLGKTVKFKMNRFGNKDSTYLVYLDKEQSKFLCDCRQFERIGIPCSHIICAMRQEHMTEFPDSLICKRWTKSAKDDYISSMSSKECDDEKMFMFRRGTMSTALNNLYDISCKHPDDYKEAMEGIYNLCEKIKRRRESGEKYKTNPSVIGDPIKAKAKGAPRKKKKTSRKRRHCSLCKNIAHTKQNCPELAGRDVLNEVNEEDYSRRSDHDKNVGCEGVDKSCDRKRKRKVVNVTSMQEDINKRISKQIENEGIGRPNENMTDKNMHTGFDNSQDQGVVHDNPPVKMEDQNVTWKDGSMYVPNYFTGANGVPIQSVPIFPQFPNQIASTFPQFFPNQGASIFPQFPRADNFPSHSSSVGYGRAHNVPHHTTGTSRPTRYHVLLDEIGFSGSSHDINASPIPQLPKLQDSVCNSMFFV