Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064953.t1 | KEH41183.1 | 91 | 89 | 8 | 0 | 1 | 89 | 25 | 113 | 9.80E-40 | 172.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064953.t1 | Q84R27 | 36.0 | 86 | 53 | 1 | 2 | 87 | 46 | 129 | 2.7e-06 | 53.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064953.t1 | A0A072VIA5 | 91.0 | 89 | 8 | 0 | 1 | 89 | 25 | 113 | 7.0e-40 | 172.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene064953.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064953.t1 | MTR_1g046320 | 91.954 | 87 | 7 | 0 | 1 | 87 | 25 | 111 | 8.82e-54 | 172 |
| MS.gene064953.t1 | MTR_1g046200 | 84.524 | 84 | 13 | 0 | 2 | 85 | 23 | 106 | 1.13e-45 | 151 |
| MS.gene064953.t1 | MTR_1g046340 | 73.958 | 96 | 25 | 0 | 1 | 96 | 25 | 120 | 4.09e-45 | 150 |
| MS.gene064953.t1 | MTR_3g088515 | 75.309 | 81 | 20 | 0 | 1 | 81 | 36 | 116 | 5.30e-38 | 129 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCAAGTTCACAAAAGCATT+TGG | 0.331753 | 1.4:-84873897 | None:intergenic |
| CGAGGAGGAAAATAATAATA+TGG | 0.341479 | 1.4:+84874160 | MS.gene064953:CDS |
| TAGAGAAGAAAATTGTTTAG+TGG | 0.355645 | 1.4:+84874040 | MS.gene064953:CDS |
| TGAACTTGGTGCGTTCAAAA+AGG | 0.361173 | 1.4:+84873910 | MS.gene064953:CDS |
| GATGGTGATGATGTTGATTA+TGG | 0.366118 | 1.4:+84874203 | MS.gene064953:CDS |
| CTCTTCCTCTACGATCTCTT+AGG | 0.380260 | 1.4:-84873953 | None:intergenic |
| GGAAGATGAGGAAGAGGAAG+AGG | 0.385448 | 1.4:+84874181 | MS.gene064953:CDS |
| CCAAGTTCACAAAAGCATTT+GGG | 0.414978 | 1.4:-84873896 | None:intergenic |
| CATGTCAAAGCGCGTCGTAT+TGG | 0.452247 | 1.4:+84873981 | MS.gene064953:CDS |
| TAATATGGAAGATGAGGAAG+AGG | 0.461266 | 1.4:+84874175 | MS.gene064953:CDS |
| GAGATCGTAGAGGAAGAGAT+TGG | 0.486399 | 1.4:+84873958 | MS.gene064953:CDS |
| GATGAGGAAGAGGAAGAGGA+TGG | 0.519418 | 1.4:+84874185 | MS.gene064953:CDS |
| ATCAATGTGTGAAAAGATGC+CGG | 0.534658 | 1.4:+84874130 | MS.gene064953:CDS |
| GTGTGAAAAGATGCCGGAGA+TGG | 0.558932 | 1.4:+84874136 | MS.gene064953:CDS |
| AAATAATAATATGGAAGATG+AGG | 0.565051 | 1.4:+84874169 | MS.gene064953:CDS |
| GACGATGAGGGTGATGATGA+GGG | 0.579768 | 1.4:+84874272 | MS.gene064953:CDS |
| AAAGATGCCGGAGATGGACG+AGG | 0.591612 | 1.4:+84874142 | MS.gene064953:CDS |
| TGACGATGAGGGTGATGATG+AGG | 0.595776 | 1.4:+84874271 | MS.gene064953:CDS |
| TGATGATGATGATGCTGAAG+AGG | 0.610168 | 1.4:+84874238 | MS.gene064953:CDS |
| GATGATGATGATGCTGAAGA+GGG | 0.617100 | 1.4:+84874239 | MS.gene064953:CDS |
| GGTGATGATAGTGACGATGA+GGG | 0.623737 | 1.4:+84874260 | MS.gene064953:CDS |
| GGGTGATGATAGTGACGATG+AGG | 0.647049 | 1.4:+84874259 | MS.gene064953:CDS |
| GATGCCGGAGATGGACGAGG+AGG | 0.688968 | 1.4:+84874145 | MS.gene064953:CDS |
| TTTATCCTAAGAGATCGTAG+AGG | 0.735718 | 1.4:+84873948 | MS.gene064953:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAATAATATGGAAGATG+AGG | + | chr1.4:84874169-84874188 | MS.gene064953:CDS | 20.0% |
| !! | TTTCAATATGAGTAAAAACA+AGG | - | chr1.4:84874076-84874095 | None:intergenic | 20.0% |
| ! | TAGAGAAGAAAATTGTTTAG+TGG | + | chr1.4:84874040-84874059 | MS.gene064953:CDS | 25.0% |
| !!! | AGTTTTATTTTGATGATGGT+TGG | + | chr1.4:84874009-84874028 | MS.gene064953:CDS | 25.0% |
| CGAGGAGGAAAATAATAATA+TGG | + | chr1.4:84874160-84874179 | MS.gene064953:CDS | 30.0% | |
| TTCAAAAAGGAGGGTAATAA+AGG | + | chr1.4:84873923-84873942 | MS.gene064953:CDS | 30.0% | |
| !!! | GACGAGTTTTATTTTGATGA+TGG | + | chr1.4:84874005-84874024 | MS.gene064953:CDS | 30.0% |
| ATCAATGTGTGAAAAGATGC+CGG | + | chr1.4:84874130-84874149 | MS.gene064953:CDS | 35.0% | |
| TAATATGGAAGATGAGGAAG+AGG | + | chr1.4:84874175-84874194 | MS.gene064953:CDS | 35.0% | |
| TTTATCCTAAGAGATCGTAG+AGG | + | chr1.4:84873948-84873967 | MS.gene064953:CDS | 35.0% | |
| ! | GATGGTGATGATGTTGATTA+TGG | + | chr1.4:84874203-84874222 | MS.gene064953:CDS | 35.0% |
| !! | ACCAAGTTCACAAAAGCATT+TGG | - | chr1.4:84873900-84873919 | None:intergenic | 35.0% |
| !! | CCAAGTTCACAAAAGCATTT+GGG | - | chr1.4:84873899-84873918 | None:intergenic | 35.0% |
| ! | CCCAAATGCTTTTGTGAACT+TGG | + | chr1.4:84873896-84873915 | MS.gene064953:CDS | 40.0% |
| ! | GATGATGATGATGCTGAAGA+GGG | + | chr1.4:84874239-84874258 | MS.gene064953:CDS | 40.0% |
| ! | TGATGATGATGATGCTGAAG+AGG | + | chr1.4:84874238-84874257 | MS.gene064953:CDS | 40.0% |
| !! | TGAACTTGGTGCGTTCAAAA+AGG | + | chr1.4:84873910-84873929 | MS.gene064953:CDS | 40.0% |
| CTCTTCCTCTACGATCTCTT+AGG | - | chr1.4:84873956-84873975 | None:intergenic | 45.0% | |
| GAGATCGTAGAGGAAGAGAT+TGG | + | chr1.4:84873958-84873977 | MS.gene064953:CDS | 45.0% | |
| ! | CTTGGTGCGTTCAAAAAGGA+GGG | + | chr1.4:84873914-84873933 | MS.gene064953:CDS | 45.0% |
| ! | GGTGATGATAGTGACGATGA+GGG | + | chr1.4:84874260-84874279 | MS.gene064953:CDS | 45.0% |
| !! | ACTTGGTGCGTTCAAAAAGG+AGG | + | chr1.4:84873913-84873932 | MS.gene064953:CDS | 45.0% |
| CATGTCAAAGCGCGTCGTAT+TGG | + | chr1.4:84873981-84874000 | MS.gene064953:CDS | 50.0% | |
| GATGAGGAAGAGGAAGAGGA+TGG | + | chr1.4:84874185-84874204 | MS.gene064953:CDS | 50.0% | |
| GGAAGATGAGGAAGAGGAAG+AGG | + | chr1.4:84874181-84874200 | MS.gene064953:CDS | 50.0% | |
| GTGTGAAAAGATGCCGGAGA+TGG | + | chr1.4:84874136-84874155 | MS.gene064953:CDS | 50.0% | |
| ! | GGGTGATGATAGTGACGATG+AGG | + | chr1.4:84874259-84874278 | MS.gene064953:CDS | 50.0% |
| ! | TGACGATGAGGGTGATGATG+AGG | + | chr1.4:84874271-84874290 | MS.gene064953:CDS | 50.0% |
| ! | TTTTCCTCCTCGTCCATCTC+CGG | - | chr1.4:84874152-84874171 | None:intergenic | 50.0% |
| AAAGATGCCGGAGATGGACG+AGG | + | chr1.4:84874142-84874161 | MS.gene064953:CDS | 55.0% | |
| GATGCCGGAGATGGACGAGG+AGG | + | chr1.4:84874145-84874164 | MS.gene064953:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 84873891 | 84874293 | 84873891 | ID=MS.gene064953 |
| chr1.4 | mRNA | 84873891 | 84874293 | 84873891 | ID=MS.gene064953.t1;Parent=MS.gene064953 |
| chr1.4 | exon | 84873891 | 84874293 | 84873891 | ID=MS.gene064953.t1.exon1;Parent=MS.gene064953.t1 |
| chr1.4 | CDS | 84873891 | 84874293 | 84873891 | ID=cds.MS.gene064953.t1;Parent=MS.gene064953.t1 |
| Gene Sequence |
| Protein sequence |
