Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064955.t1 | KEH41184.1 | 93 | 86 | 6 | 0 | 1 | 86 | 25 | 110 | 3.60E-38 | 167.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064955.t1 | A0A072VGK8 | 93.0 | 86 | 6 | 0 | 1 | 86 | 25 | 110 | 2.6e-38 | 167.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene064955.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene064955.t1 | MTR_1g046340 | 93.878 | 98 | 6 | 0 | 1 | 98 | 25 | 122 | 8.67e-62 | 193 |
| MS.gene064955.t1 | MTR_3g088515 | 85.185 | 81 | 12 | 0 | 1 | 81 | 36 | 116 | 3.21e-45 | 148 |
| MS.gene064955.t1 | MTR_1g046200 | 74.713 | 87 | 22 | 0 | 2 | 88 | 23 | 109 | 2.15e-40 | 138 |
| MS.gene064955.t1 | MTR_1g046320 | 71.591 | 88 | 25 | 0 | 1 | 88 | 25 | 112 | 3.75e-39 | 135 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACTTCCTCTATGATCTCTT+AGG | 0.351749 | 1.4:-31022917 | None:intergenic |
| AAGGAAGTCATTGTTCTCTA+AGG | 0.370651 | 1.4:-31023018 | None:intergenic |
| AGAGGATGAGGAAGAGGAAG+AGG | 0.437743 | 1.4:+31023148 | MS.gene064955:CDS |
| TTCAAAACAAAGGCTGTTGA+AGG | 0.442905 | 1.4:+31022887 | MS.gene064955:CDS |
| AATAAAGTTTGTTGCCGATA+GGG | 0.473847 | 1.4:-31022959 | None:intergenic |
| GAGGAAGAGGAAGAGGAAGA+TGG | 0.482567 | 1.4:+31023155 | MS.gene064955:CDS |
| CATGTCAAAGTGCGCCCTAT+CGG | 0.483624 | 1.4:+31022945 | MS.gene064955:CDS |
| TACGATAGAGGATGAGGAAG+AGG | 0.486893 | 1.4:+31023142 | MS.gene064955:CDS |
| CGTTGATGAGGAGGAGGATG+AGG | 0.496617 | 1.4:+31023247 | MS.gene064955:CDS |
| GGAAAATACTAATACGATAG+AGG | 0.497125 | 1.4:+31023130 | MS.gene064955:CDS |
| CTCAATGTGTCAAAAGATGA+AGG | 0.502234 | 1.4:+31023094 | MS.gene064955:CDS |
| GAGATCATAGAGGAAGTGAT+TGG | 0.510228 | 1.4:+31022922 | MS.gene064955:CDS |
| GACACATTGAGGATAGTTCA+AGG | 0.516055 | 1.4:-31023082 | None:intergenic |
| GTGTCAAAAGATGAAGGTAA+TGG | 0.520383 | 1.4:+31023100 | MS.gene064955:CDS |
| TACTAATACGATAGAGGATG+AGG | 0.521923 | 1.4:+31023136 | MS.gene064955:CDS |
| ATCAGCTTCACAAAAGCCGT+TGG | 0.529706 | 1.4:-31022861 | None:intergenic |
| CGATGACGTTGATGAGGAGG+AGG | 0.543560 | 1.4:+31023241 | MS.gene064955:CDS |
| TCAGCTTCACAAAAGCCGTT+GGG | 0.551363 | 1.4:-31022860 | None:intergenic |
| TTTCAATATGTGTGAAAACA+AGG | 0.554136 | 1.4:-31023037 | None:intergenic |
| AAATAAAGTTTGTTGCCGAT+AGG | 0.559250 | 1.4:-31022960 | None:intergenic |
| TGATGATTACACAATGATAG+AGG | 0.564788 | 1.4:+31023196 | MS.gene064955:CDS |
| ATGATAGAGGAAGAAGAAGA+TGG | 0.574971 | 1.4:+31023209 | MS.gene064955:CDS |
| ATGGTAGATGAAGAAGAAGA+TGG | 0.592976 | 1.4:+31023299 | MS.gene064955:CDS |
| AGCTGATGCTTTCAAAACAA+AGG | 0.597947 | 1.4:+31022877 | MS.gene064955:CDS |
| TCTGCTGCAGCTTATCCCAA+CGG | 0.606121 | 1.4:+31022845 | None:intergenic |
| GATGAAGGTAATGGACGAGG+AGG | 0.612327 | 1.4:+31023109 | MS.gene064955:CDS |
| TAACGATGACGTTGATGAGG+AGG | 0.620129 | 1.4:+31023238 | MS.gene064955:CDS |
| TTTATCCTAAGAGATCATAG+AGG | 0.632452 | 1.4:+31022912 | MS.gene064955:CDS |
| AAAGATGAAGGTAATGGACG+AGG | 0.655125 | 1.4:+31023106 | MS.gene064955:CDS |
| TGATAACGATGACGTTGATG+AGG | 0.693089 | 1.4:+31023235 | MS.gene064955:CDS |
| TGATGATGATGATTACACAA+TGG | 0.769323 | 1.4:+31023280 | MS.gene064955:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACAAACTTTATTTTGATGA+TGG | + | chr1.4:31022969-31022988 | MS.gene064955:CDS | 20.0% |
| ! | TTTCAATATGTGTGAAAACA+AGG | - | chr1.4:31023040-31023059 | None:intergenic | 25.0% |
| !!! | AACTTTATTTTGATGATGGT+TGG | + | chr1.4:31022973-31022992 | MS.gene064955:CDS | 25.0% |
| GGAAAATACTAATACGATAG+AGG | + | chr1.4:31023130-31023149 | MS.gene064955:CDS | 30.0% | |
| TGATGATTACACAATGATAG+AGG | + | chr1.4:31023196-31023215 | MS.gene064955:CDS | 30.0% | |
| TTTATCCTAAGAGATCATAG+AGG | + | chr1.4:31022912-31022931 | MS.gene064955:CDS | 30.0% | |
| ! | TGATGATGATGATTACACAA+TGG | + | chr1.4:31023190-31023209 | MS.gene064955:CDS | 30.0% |
| !! | AAATAAAGTTTGTTGCCGAT+AGG | - | chr1.4:31022963-31022982 | None:intergenic | 30.0% |
| !! | AATAAAGTTTGTTGCCGATA+GGG | - | chr1.4:31022962-31022981 | None:intergenic | 30.0% |
| AAGGAAGTCATTGTTCTCTA+AGG | - | chr1.4:31023021-31023040 | None:intergenic | 35.0% | |
| AGCTGATGCTTTCAAAACAA+AGG | + | chr1.4:31022877-31022896 | MS.gene064955:CDS | 35.0% | |
| ATGATAGAGGAAGAAGAAGA+TGG | + | chr1.4:31023209-31023228 | MS.gene064955:CDS | 35.0% | |
| CTCAATGTGTCAAAAGATGA+AGG | + | chr1.4:31023094-31023113 | MS.gene064955:CDS | 35.0% | |
| GTGTCAAAAGATGAAGGTAA+TGG | + | chr1.4:31023100-31023119 | MS.gene064955:CDS | 35.0% | |
| TACTAATACGATAGAGGATG+AGG | + | chr1.4:31023136-31023155 | MS.gene064955:CDS | 35.0% | |
| TTCAAAACAAAGGCTGTTGA+AGG | + | chr1.4:31022887-31022906 | MS.gene064955:CDS | 35.0% | |
| ! | CTTCATCTTTTGACACATTG+AGG | - | chr1.4:31023096-31023115 | None:intergenic | 35.0% |
| CACTTCCTCTATGATCTCTT+AGG | - | chr1.4:31022920-31022939 | None:intergenic | 40.0% | |
| GACACATTGAGGATAGTTCA+AGG | - | chr1.4:31023085-31023104 | None:intergenic | 40.0% | |
| GAGATCATAGAGGAAGTGAT+TGG | + | chr1.4:31022922-31022941 | MS.gene064955:CDS | 40.0% | |
| ! | TGATAACGATGACGTTGATG+AGG | + | chr1.4:31023235-31023254 | MS.gene064955:CDS | 40.0% |
| !! | AAAGATGAAGGTAATGGACG+AGG | + | chr1.4:31023106-31023125 | MS.gene064955:CDS | 40.0% |
| ATCAGCTTCACAAAAGCCGT+TGG | - | chr1.4:31022864-31022883 | None:intergenic | 45.0% | |
| TACGATAGAGGATGAGGAAG+AGG | + | chr1.4:31023142-31023161 | MS.gene064955:CDS | 45.0% | |
| TCAGCTTCACAAAAGCCGTT+GGG | - | chr1.4:31022863-31022882 | None:intergenic | 45.0% | |
| ! | TAACGATGACGTTGATGAGG+AGG | + | chr1.4:31023238-31023257 | MS.gene064955:CDS | 45.0% |
| AGAGGATGAGGAAGAGGAAG+AGG | + | chr1.4:31023148-31023167 | MS.gene064955:CDS | 50.0% | |
| CATGTCAAAGTGCGCCCTAT+CGG | + | chr1.4:31022945-31022964 | MS.gene064955:CDS | 50.0% | |
| GAGGAAGAGGAAGAGGAAGA+TGG | + | chr1.4:31023155-31023174 | MS.gene064955:CDS | 50.0% | |
| !! | GATGAAGGTAATGGACGAGG+AGG | + | chr1.4:31023109-31023128 | MS.gene064955:CDS | 50.0% |
| ! | CGATGACGTTGATGAGGAGG+AGG | + | chr1.4:31023241-31023260 | MS.gene064955:CDS | 55.0% |
| ! | CGTTGATGAGGAGGAGGATG+AGG | + | chr1.4:31023247-31023266 | MS.gene064955:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 31022855 | 31023320 | 31022855 | ID=MS.gene064955 |
| chr1.4 | mRNA | 31022855 | 31023320 | 31022855 | ID=MS.gene064955.t1;Parent=MS.gene064955 |
| chr1.4 | exon | 31022855 | 31023320 | 31022855 | ID=MS.gene064955.t1.exon1;Parent=MS.gene064955.t1 |
| chr1.4 | CDS | 31022855 | 31023320 | 31022855 | ID=cds.MS.gene064955.t1;Parent=MS.gene064955.t1 |
| Gene Sequence |
| Protein sequence |
