AlfalfaGEDB | MS.gene06496

M. sativa cultivar XinJiangDaYe / MS.gene06496

KEGG: -
GO: -
PFAM: PF03195.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06496.t1	XP_003602464.1	100	103	0	0	1	103	30	132	6.10E-53	216.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06496.t1	Q8L5T5	94.1	101	6	0	3	103	30	130	1.2e-49	196.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06496.t1	G7J9S3	100.0	103	0	0	1	103	30	132	4.4e-53	216.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene06496.t1	TF	LOB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06496.t1	MTR_3g094690	100.000	103	0	0	1	103	30	132	2.43e-74	220
MS.gene06496.t1	MTR_3g094690	99.029	103	0	1	1	103	30	131	1.11e-71	213
MS.gene06496.t1	MTR_5g083960	91.837	98	8	0	6	103	27	124	4.91e-64	192
MS.gene06496.t1	MTR_5g075020	82.828	99	15	1	1	97	24	122	1.92e-58	179
MS.gene06496.t1	MTR_5g075020	82.828	99	15	1	1	97	28	126	3.62e-58	179
MS.gene06496.t1	MTR_5g083230	74.157	89	23	0	17	105	5	93	2.05e-44	142
MS.gene06496.t1	MTR_4g083680	72.414	87	24	0	17	103	14	100	1.15e-43	140
MS.gene06496.t1	MTR_3g071590	70.652	92	27	0	14	105	4	95	1.36e-43	139
MS.gene06496.t1	MTR_2g068760	70.787	89	26	0	15	103	10	98	1.75e-43	139
MS.gene06496.t1	MTR_7g033800	67.816	87	28	0	17	103	10	96	4.44e-40	131
MS.gene06496.t1	MTR_3g031660	67.416	89	29	0	17	105	6	94	5.78e-40	130
MS.gene06496.t1	MTR_6g005070	66.667	87	29	0	17	103	8	94	6.06e-40	130
MS.gene06496.t1	MTR_6g005080	64.368	87	31	0	17	103	9	95	1.70e-38	126
MS.gene06496.t1	MTR_4g105170	66.667	87	29	0	17	103	10	96	2.05e-38	127
MS.gene06496.t1	MTR_3g077240	66.667	87	29	0	17	103	9	95	3.78e-38	127
MS.gene06496.t1	MTR_2g093310	61.364	88	34	0	16	103	31	118	3.67e-37	124
MS.gene06496.t1	MTR_3g073690	64.368	87	31	0	17	103	8	94	9.15e-37	123
MS.gene06496.t1	MTR_3g452660	63.218	87	32	0	17	103	6	92	1.27e-36	125
MS.gene06496.t1	MTR_5g017950	65.517	87	30	0	17	103	7	93	1.32e-36	126
MS.gene06496.t1	MTR_3g071420	64.368	87	31	0	17	103	13	99	3.32e-36	121
MS.gene06496.t1	MTR_8g040900	64.634	82	29	0	17	98	6	87	3.93e-36	122
MS.gene06496.t1	MTR_5g083010	64.706	85	30	0	19	103	12	96	6.05e-35	118
MS.gene06496.t1	MTR_1g095850	62.069	87	33	0	19	105	73	159	8.43e-35	119
MS.gene06496.t1	MTR_4g060950	60.674	89	34	1	16	103	22	110	2.52e-34	117
MS.gene06496.t1	MTR_7g074990	64.368	87	30	1	18	103	20	106	3.07e-33	115
MS.gene06496.t1	MTR_7g096530	55.914	93	40	1	15	106	11	103	1.03e-31	111
MS.gene06496.t1	MTR_7g028905	57.647	85	36	0	19	103	6	90	1.07e-31	108
MS.gene06496.t1	MTR_5g093010	56.471	85	37	0	19	103	6	90	9.12e-30	103
MS.gene06496.t1	MTR_1g070220	57.471	87	36	1	18	103	15	101	1.70e-29	105
MS.gene06496.t1	MTR_6g027710	57.303	89	37	1	16	103	38	126	3.02e-29	105
MS.gene06496.t1	MTR_8g036105	58.621	87	35	1	18	103	18	104	5.17e-28	101
MS.gene06496.t1	MTR_1g070205	55.682	88	36	2	18	103	12	98	1.09e-27	101
MS.gene06496.t1	MTR_7g096610	55.056	89	37	2	17	103	7	94	3.24e-27	99.8
MS.gene06496.t1	MTR_8g036085	55.172	87	38	1	18	103	23	109	3.94e-27	99.8
MS.gene06496.t1	MTR_5g080470	51.136	88	42	1	17	103	6	93	7.63e-27	98.2
MS.gene06496.t1	MTR_8g079660	52.273	88	42	0	16	103	6	93	7.71e-27	99.8
MS.gene06496.t1	MTR_6g011250	45.000	80	43	1	19	97	10	89	5.20e-24	90.5
MS.gene06496.t1	MTR_7g075230	52.809	89	39	2	18	103	12	100	4.74e-23	88.6
MS.gene06496.t1	MTR_8g079620	46.512	86	46	0	16	101	6	91	1.15e-22	88.2
MS.gene06496.t1	MTR_8g079580	51.136	88	42	1	16	102	6	93	1.74e-22	87.8
MS.gene06496.t1	MTR_6g027700	45.977	87	46	1	18	103	7	93	3.82e-22	86.3
MS.gene06496.t1	MTR_6g011230	42.857	84	48	0	14	97	7	90	4.97e-22	85.9
MS.gene06496.t1	MTR_6g011200	41.573	89	48	2	13	97	1	89	2.40e-21	85.1
MS.gene06496.t1	MTR_4g107450	42.697	89	51	0	15	103	7	95	9.83e-20	81.6
MS.gene06496.t1	MTR_5g085390	40.449	89	49	2	13	97	1	89	3.66e-19	79.0
MS.gene06496.t1	MTR_4g099230	41.250	80	46	1	19	97	3	82	3.95e-18	74.7
MS.gene06496.t1	MTR_8g017090	40.000	90	52	2	15	103	9	97	1.45e-17	75.9
MS.gene06496.t1	MTR_2g100020	40.000	90	52	2	15	103	16	104	9.72e-17	73.2
MS.gene06496.t1	MTR_4g088035	34.483	87	57	0	17	103	5	91	5.55e-15	68.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06496.t1	AT2G40470	93.750	96	6	0	3	98	5	100	1.72e-62	189
MS.gene06496.t1	AT2G40470	93.750	96	6	0	3	98	30	125	2.55e-62	189
MS.gene06496.t1	AT2G30340	82.857	105	16	2	1	103	33	137	5.96e-60	184
MS.gene06496.t1	AT2G30340	82.857	105	16	2	1	103	34	138	7.39e-60	184
MS.gene06496.t1	AT1G31320	73.563	87	23	0	17	103	12	98	6.11e-43	138
MS.gene06496.t1	AT2G30130	67.677	99	28	1	7	105	1	95	6.45e-43	139
MS.gene06496.t1	AT3G27650	62.626	99	37	0	5	103	26	124	5.25e-41	133
MS.gene06496.t1	AT5G66870	68.966	87	27	0	17	103	6	92	6.00e-39	132
MS.gene06496.t1	AT1G16530	62.222	90	33	1	15	103	11	100	1.97e-38	126
MS.gene06496.t1	AT1G65620	67.059	85	28	0	14	98	5	89	6.58e-38	126
MS.gene06496.t1	AT1G65620	67.059	85	28	0	14	98	5	89	6.58e-38	126
MS.gene06496.t1	AT1G65620	67.059	85	28	0	14	98	5	89	6.58e-38	126
MS.gene06496.t1	AT1G65620	67.059	85	28	0	14	98	5	89	6.58e-38	126
MS.gene06496.t1	AT1G65620	67.059	85	28	0	14	98	5	89	6.58e-38	126
MS.gene06496.t1	AT5G63090	65.517	87	30	0	17	103	10	96	1.11e-37	125
MS.gene06496.t1	AT5G63090	65.517	87	30	0	17	103	10	96	1.11e-37	125
MS.gene06496.t1	AT5G63090	65.517	87	30	0	17	103	10	96	1.11e-37	125
MS.gene06496.t1	AT5G63090	65.517	87	30	0	17	103	10	96	1.11e-37	125
MS.gene06496.t1	AT1G07900	60.440	91	36	0	15	105	30	120	3.89e-37	124
MS.gene06496.t1	AT2G23660	65.116	86	30	0	17	102	4	89	9.18e-37	126
MS.gene06496.t1	AT2G23660	65.116	86	30	0	17	102	4	89	9.18e-37	126
MS.gene06496.t1	AT2G23660	65.116	86	30	0	17	102	4	89	9.18e-37	126
MS.gene06496.t1	AT2G28500	61.111	90	35	0	16	105	53	142	1.09e-36	124
MS.gene06496.t1	AT3G11090	63.529	85	31	0	19	103	12	96	5.04e-34	115
MS.gene06496.t1	AT3G26620	58.824	85	35	0	19	103	6	90	2.04e-33	112
MS.gene06496.t1	AT3G26660	58.824	85	35	0	19	103	6	90	2.06e-33	112
MS.gene06496.t1	AT2G42430	54.639	97	43	1	8	103	5	101	6.45e-31	109
MS.gene06496.t1	AT4G00210	58.621	87	35	1	18	103	11	97	9.18e-30	106
MS.gene06496.t1	AT5G06080	55.682	88	38	1	17	103	6	93	5.94e-28	100
MS.gene06496.t1	AT3G03760	55.682	88	38	1	17	103	50	137	2.01e-27	101
MS.gene06496.t1	AT3G58190	55.682	88	36	2	18	103	11	97	2.15e-27	100
MS.gene06496.t1	AT2G31310	50.000	88	43	1	17	103	6	93	6.11e-27	98.2
MS.gene06496.t1	AT4G00220	57.143	84	35	1	21	103	20	103	7.94e-27	99.0
MS.gene06496.t1	AT2G45420	54.762	84	37	1	21	103	40	123	3.56e-26	98.2
MS.gene06496.t1	AT2G42440	51.685	89	40	2	17	103	6	93	1.59e-25	95.9
MS.gene06496.t1	AT2G45410	53.571	84	38	1	21	103	19	102	7.42e-25	92.8
MS.gene06496.t1	AT3G50510	47.126	87	46	0	17	103	11	97	6.52e-24	90.9
MS.gene06496.t1	AT3G50510	47.126	87	46	0	17	103	11	97	6.52e-24	90.9
MS.gene06496.t1	AT3G47870	43.373	83	47	0	19	101	37	119	2.16e-22	89.4
MS.gene06496.t1	AT1G06280	48.052	77	40	0	19	95	25	101	3.39e-20	81.3
MS.gene06496.t1	AT1G72980	41.379	87	51	0	15	101	10	96	2.68e-18	76.6
MS.gene06496.t1	AT3G13850	38.824	85	52	0	19	103	37	121	3.89e-18	77.0
MS.gene06496.t1	AT5G35900	39.080	87	53	0	17	103	4	90	7.16e-18	75.1
MS.gene06496.t1	AT2G19820	34.483	87	56	1	18	103	12	98	7.31e-15	65.5
MS.gene06496.t1	AT5G15060	34.211	76	48	1	16	89	15	90	6.44e-11	55.8

Find 23 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACAAGGGGTTATAGTATTT+AGG	0.181231	3.2:+79166028	None:intergenic
GGGTTCATGAGGAGAGAAAT+AGG	0.290029	3.2:+79165954	None:intergenic
AGGCTAAGAGATCCAGTTTA+TGG	0.306813	3.2:-79165725	MS.gene06496:CDS
ACAAGGGGTTATAGTATTTA+GGG	0.313905	3.2:+79166029	None:intergenic
TGTGAACAGAAGCAAACTTC+TGG	0.320592	3.2:+79165932	None:intergenic
GTTATAGTATTTAGGGTTCC+TGG	0.351578	3.2:+79166036	None:intergenic
GCACCCATGCATCCATAAAC+TGG	0.381798	3.2:+79165713	None:intergenic
GGAGAGAAATAGGGAGAAAA+GGG	0.384566	3.2:+79165964	None:intergenic
AGGAGAGAAATAGGGAGAAA+AGG	0.419838	3.2:+79165963	None:intergenic
GTGAACAGAAGCAAACTTCT+GGG	0.421575	3.2:+79165933	None:intergenic
AATTTCTGCATTGCAACAAC+AGG	0.457176	3.2:-79165690	MS.gene06496:intron
ATGGGAAGAAGACATATGTT+AGG	0.472989	3.2:-79166063	MS.gene06496:CDS
GGTTCATGAGGAGAGAAATA+GGG	0.495022	3.2:+79165955	None:intergenic
AGATCCAGTTTATGGATGCA+TGG	0.526865	3.2:-79165717	MS.gene06496:CDS
AATAGCTTACATGCTGCACA+AGG	0.566069	3.2:+79166012	None:intergenic
GATCCAGTTTATGGATGCAT+GGG	0.578062	3.2:-79165716	MS.gene06496:CDS
GCAAACTTCTGGGGTTCATG+AGG	0.589878	3.2:+79165943	None:intergenic
TAGCTTACATGCTGCACAAG+GGG	0.599040	3.2:+79166014	None:intergenic
ATAGTATTTAGGGTTCCTGG+TGG	0.604329	3.2:+79166039	None:intergenic
TGAACAGAAGCAAACTTCTG+GGG	0.623533	3.2:+79165934	None:intergenic
AGACATATGTTAGGTCCACC+AGG	0.627473	3.2:-79166054	MS.gene06496:CDS
TTGTTTATGAAGCAAATGTG+AGG	0.672469	3.2:-79165745	MS.gene06496:CDS
ATAGCTTACATGCTGCACAA+GGG	0.680325	3.2:+79166013	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATACTTAAATACAAATTA+AGG	-	chr3.2:79166052-79166071	MS.gene06496:CDS	10.0%
!!	AATACTTAAATACAAATTAA+GGG	-	chr3.2:79166053-79166072	MS.gene06496:CDS	10.0%
!!!	AATATATATAATGTTTTAGG+AGG	-	chr3.2:79165876-79165895	MS.gene06496:intron	15.0%
	ACAAGGGGTTATAGTATTTA+GGG	+	chr3.2:79165654-79165673	None:intergenic	30.0%
	TTGTTTATGAAGCAAATGTG+AGG	-	chr3.2:79165935-79165954	MS.gene06496:CDS	30.0%
	AATTTCTGCATTGCAACAAC+AGG	-	chr3.2:79165990-79166009	MS.gene06496:CDS	35.0%
	ATGGGAAGAAGACATATGTT+AGG	-	chr3.2:79165617-79165636	MS.gene06496:intron	35.0%
	CAACGTCTCAAAAATGCTAA+TGG	-	chr3.2:79165787-79165806	MS.gene06496:CDS	35.0%
	CACAAGGGGTTATAGTATTT+AGG	+	chr3.2:79165655-79165674	None:intergenic	35.0%
	GCAGAACTCAATCTATTAAG+AGG	-	chr3.2:79166027-79166046	MS.gene06496:CDS	35.0%
!	GCTTCTGTTCACAAAGTTTT+TGG	-	chr3.2:79165758-79165777	MS.gene06496:CDS	35.0%
!	GTTATAGTATTTAGGGTTCC+TGG	+	chr3.2:79165647-79165666	None:intergenic	35.0%
	AATAGCTTACATGCTGCACA+AGG	+	chr3.2:79165671-79165690	None:intergenic	40.0%
	AGATCCAGTTTATGGATGCA+TGG	-	chr3.2:79165963-79165982	MS.gene06496:CDS	40.0%
	AGGAGAGAAATAGGGAGAAA+AGG	+	chr3.2:79165720-79165739	None:intergenic	40.0%
	AGGCTAAGAGATCCAGTTTA+TGG	-	chr3.2:79165955-79165974	MS.gene06496:CDS	40.0%
	ATAGCTTACATGCTGCACAA+GGG	+	chr3.2:79165670-79165689	None:intergenic	40.0%
	GATCCAGTTTATGGATGCAT+GGG	-	chr3.2:79165964-79165983	MS.gene06496:CDS	40.0%
	GGAGAGAAATAGGGAGAAAA+GGG	+	chr3.2:79165719-79165738	None:intergenic	40.0%
	GGTTCATGAGGAGAGAAATA+GGG	+	chr3.2:79165728-79165747	None:intergenic	40.0%
	GTGAACAGAAGCAAACTTCT+GGG	+	chr3.2:79165750-79165769	None:intergenic	40.0%
	TGAACAGAAGCAAACTTCTG+GGG	+	chr3.2:79165749-79165768	None:intergenic	40.0%
	TGTGAACAGAAGCAAACTTC+TGG	+	chr3.2:79165751-79165770	None:intergenic	40.0%
!	ATAGTATTTAGGGTTCCTGG+TGG	+	chr3.2:79165644-79165663	None:intergenic	40.0%
!!!	TAGCATTTTTGAGACGTTGC+TGG	+	chr3.2:79165786-79165805	None:intergenic	40.0%
	AGACATATGTTAGGTCCACC+AGG	-	chr3.2:79165626-79165645	MS.gene06496:intron	45.0%
	GGGTTCATGAGGAGAGAAAT+AGG	+	chr3.2:79165729-79165748	None:intergenic	45.0%
	TAGCTTACATGCTGCACAAG+GGG	+	chr3.2:79165669-79165688	None:intergenic	45.0%
!!!	AATAATATATATAATGTTTT+AGG	-	chr3.2:79165873-79165892	MS.gene06496:intron	5.0%
	GCACCCATGCATCCATAAAC+TGG	+	chr3.2:79165970-79165989	None:intergenic	50.0%
!!	GCAAACTTCTGGGGTTCATG+AGG	+	chr3.2:79165740-79165759	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	79165617	79166085	79165617	ID=MS.gene06496
chr3.2	mRNA	79165617	79166085	79165617	ID=MS.gene06496.t1;Parent=MS.gene06496
chr3.2	exon	79165894	79166085	79165894	ID=MS.gene06496.t1.exon1;Parent=MS.gene06496.t1
chr3.2	CDS	79165894	79166085	79165894	ID=cds.MS.gene06496.t1;Parent=MS.gene06496.t1
chr3.2	exon	79165691	79165807	79165691	ID=MS.gene06496.t1.exon2;Parent=MS.gene06496.t1
chr3.2	CDS	79165691	79165807	79165691	ID=cds.MS.gene06496.t1;Parent=MS.gene06496.t1
chr3.2	exon	79165617	79165628	79165617	ID=MS.gene06496.t1.exon3;Parent=MS.gene06496.t1
chr3.2	CDS	79165617	79165628	79165617	ID=cds.MS.gene06496.t1;Parent=MS.gene06496.t1

Gene Sequence

>MS.gene06496

ATGGGAAGAAGACATATGTTAGGTCCACCAGGAACCCTAAATACTATAACCCCTTGTGCAGCATGTAAGCTATTAAGAAGAAGATGTGCTCAAGAGTGTCCCTTTTCTCCCTATTTCTCTCCTCATGAACCCCAGAAGTTTGCTTCTGTTCACAAAGTTTTTGGTGCCAGCAACGTCTCAAAAATGCTAATGGTAAACATTAATTAAATATATCTAATTATATGTCATATATTTCATATATAAAAAGCAATTAAGTAATAATATATATAATGTTTTAGGAGGTAGCAGAATGTTCAAGAGCAGATGCAGCAAATAGTCTTGTTTATGAAGCAAATGTGAGGCTAAGAGATCCAGTTTATGGATGCATGGGTGCAATTTCTGCATTGCAACAACAGGTTCAATCTTTACAAGCAGAACTCAATCTATTAAGAGGTGAAATACTTAAATACAAATTAAGGGAAGCTGCTAA

Protein sequence

>MS.gene06496.t1

MGRRHMLGPPGTLNTITPCAACKLLRRRCAQECPFSPYFSPHEPQKFASVHKVFGASNVSKMLMEVAECSRADAANSLVYEANVRLRDPVYGCMGAISALQQQGSC